mlo3 (SPBC1D7.04)


Gene Standard Namemlo3 Characterisation Statuspublished
Systematic IDSPBC1D7.04 Feature Typeprotein coding
Synonyms Name DescriptionMissegregation and Lethal when Overexpressed
ProductRNA binding protein Mlo3 Product Size199aa, 21.79 kDa
Genomic Location Chromosome II, 1744798-1743968 (831nt); CDS:1744712-1744113 (600nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding863
Annotation ExtensionEvidenceWith/FromReference
RNA binding581
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
poly(A)+ mRNA export from nucleus17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
transcription export complex5
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth on glucose carbon sourcemlo3Δ316
decreased mating efficiencymlo3Δ272
decreased vegetative cell population growthmlo3Δ652
normal growth on amphotericin Bmlo3Δ17
sensitive to bleomycinmlo3Δ78
sensitive to bortezomibmlo3Δ255
sensitive to camptothecinmlo3Δ228
sensitive to hydroxyureamlo3Δ548
sensitive to methyl methanesulfonatemlo3Δ255
sensitive to micafunginmlo3Δ119
sensitive to UV during vegetative growthmlo3Δ150
viable vegetative cell populationmlo3Δ3837

Cell Phenotype

Term NameGenotypesCount
increased spontaneous diploidizationmlo3Δ19
normal RNA level during vegetative growth122
affecting rec8mlo3Δ
affecting mei4mlo3Δ
shortened telomeresmlo3Δ39
viable curved vegetative cellmlo3Δ70
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1744798..1743968
5' UTR1744798..1744713PMID:21511999
3' UTR1744112..1743968AU009389
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00076 Pfam IPR000504 RNA recognition motif domain 57 124 65
SM00360 SMART IPR000504 RNA recognition motif domain 56 130 68
PS50102 Prosite Profiles IPR000504 RNA recognition motif domain 55 134 72
PTHR19965 HMMPANTHER 1 194 2
3.30.70.330 Gene3D IPR012677 Nucleotide-binding alpha-beta plait domain 30 133 83
SSF54928 SuperFamily IPR012677 Nucleotide-binding alpha-beta plait domain 16 131 78

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000302rrm RNA recognition motifTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000302

Protein Properties

Ave. residue weight 109.50 Da
Charge 19.00
Codon Adaptation Index 0.51
Isoelectric point 10.92
Molecular weight 21.79 kDa
Number of residues 199
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS195
present during cellular response to thiabendazole, mitotic M phaseS86
present during mitotic M phaseS2
present during mitotic M phaseS8
S195
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S195 PMID:21712547
present during cellular response to thiabendazole IDA S86 PMID:18257517
present during mitotic M phase experimental evidence S2 PMID:21712547
present during mitotic M phase experimental evidence S86 PMID:21712547
present during mitotic M phase experimental evidence S8 PMID:21712547
experimental evidence S195 PMID:24763107
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for mlo3 (SPBC1D7.04)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
110182during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
109524during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
105166during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
103038during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
27161.11during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
99958during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
28228.21during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
30during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC1D7.04 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byrmn1RNA-binding protein Affinity Capture-MSPMID:24713849
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
affinity captured byred5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
affinity captured bycid14poly(A) polymerase Cid14 Affinity Capture-WesternPMID:21436456
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured bynts1Clr6 histone deacetylase complex subunit Nts1 Affinity Capture-MSPMID:25002536
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured byair1zinc knuckle TRAMP complex subunit Air1 Affinity Capture-MSPMID:20403971
modified byclr4histone H3 lysine methyltransferase Clr4 Biochemical ActivityPMID:21436456
affinity capturesppp1pescadillo-family BRCT domain protein Ppp1 (predicted) Affinity Capture-MSPMID:21436456
affinity capturessum3translation initiation RNA helicase Sum3 Affinity Capture-MSPMID:21436456
affinity capturesSPAC1142.04Noc complex subunit Noc2 family (predicted) Affinity Capture-MSPMID:21436456
affinity capturesbrx1ribosome biogenesis protein Brx1 (predicted) Affinity Capture-MSPMID:21436456
affinity capturesgar1snoRNP pseudouridylase box H/ACA snoRNP complex protein Gar1 Affinity Capture-MSPMID:21436456
affinity capturesSPCC1322.10cell wall protein Pwp1 Affinity Capture-MSPMID:21436456
affinity capturesexo2exonuclease II Exo2 Affinity Capture-MSPMID:21436456
affinity capturesgtp1GTP binding protein Gtp1, implicated in cytoplasmic translation (predicted) Affinity Capture-MSPMID:21436456
affinity capturesnog1GTP binding protein Nog1 (predicted) Affinity Capture-MSPMID:21436456
affinity captureshas1ATP-dependent RNA helicase Has1 (predicted) Affinity Capture-MSPMID:21436456
affinity capturesrpf2Brix domain ribosome biogenesis protein Rpf2 Affinity Capture-MSPMID:21436456
affinity capturesrrs1ribosome biogenesis protein Rrs1 Affinity Capture-MSPMID:21436456
affinity capturesutp21U3 snoRNP-associated protein Utp21 (predicted) Affinity Capture-MSPMID:21436456
affinity capturespuf6pumilio family RNA-binding protein Puf6 (predicted) Affinity Capture-MSPMID:21436456
affinity capturesSPCC126.11cRNA-binding protein, rrm type Affinity Capture-MSPMID:21436456
affinity capturesSPCC306.07cU3 snoRNP-associated protein Cic1/Utp30 family (predicted) Affinity Capture-MSPMID:21436456
affinity capturesnop4RNA-binding protein Nop4 (predicted) Affinity Capture-MSPMID:21436456
affinity capturesclr4histone H3 lysine methyltransferase Clr4 Affinity Capture-WesternPMID:21436456
affinity capturesmak16nuclear HMG-like acidic protein Mak16 (predicted) Affinity Capture-MSPMID:21436456
affinity capturesutp23rRNA processing protein Utp23 (predicted) Affinity Capture-MSPMID:21436456
affinity capturesnop52nucleolar protein Nop52 family Rrp1 (predicted) Affinity Capture-MSPMID:21436456
affinity capturesrnp24RNA-binding protein Rnp24 Affinity Capture-MSPMID:21436456
affinity captureserb1WD repeat/BOP1NT protein Erb1 (predicted) Affinity Capture-MSPMID:21436456
affinity capturescid14poly(A) polymerase Cid14 Affinity Capture-MSPMID:21436456
affinity capturesSPCC16C4.16cconserved eukaryotic protein Affinity Capture-MSPMID:21436456
affinity capturesrik1silencing protein Rik1 Affinity Capture-WesternPMID:21436456
affinity capturesebp2rRNA processing protein Ebp2 (predicted) Affinity Capture-MSPMID:21436456
affinity capturessla1La protein homolog Affinity Capture-MSPMID:21436456
affinity captureskri1ribosome biogenesis protein Kri1 (predicted) Affinity Capture-MSPMID:21436456
affinity capturesnoc1ribosome biogenesis protein Noc1 (predicted) Affinity Capture-MSPMID:21436456
affinity capturesupf1ATP-dependent RNA helicase Upf1 Affinity Capture-MSPMID:21436456
affinity capturessrp2mRNA export factor Srp2 Affinity Capture-MSPMID:21436456
affinity capturessal3karyopherin Sal3 Affinity Capture-MSPMID:21436456
affinity capturesair1zinc knuckle TRAMP complex subunit Air1 Affinity Capture-MSPMID:21436456
affinity capturesmtr4ATP-dependent RNA helicase, TRAMP complex subunit Mtr4 Affinity Capture-MSPMID:21436456
affinity captureschp1chromodomain protein Chp1 Affinity Capture-WesternPMID:21436456
affinity capturesSPAC8F11.04U3 snoRNP-associated protein Cic1/Utp30 family (predicted) Affinity Capture-MSPMID:21436456
affinity capturesnop12RNA-binding protein Nop12 (predicted) Affinity Capture-MSPMID:21436456
affinity capturesnop58U3 snoRNP protein Nop58 (predicted) Affinity Capture-MSPMID:21436456
forms complex withrpn15proteasome regulatory particle, lid subcomplex subunit Rpn15/Dss1 Reconstituted ComplexPMID:15990877
forms complex withclr4histone H3 lysine methyltransferase Clr4 Reconstituted ComplexPMID:21436456
forms complex withuap56TREX complex subunit, ATP-dependent RNA helicase Uap56 Reconstituted ComplexPMID:15990877
forms complex withmex67mRNA export receptor, Tap, nucleoporin Mex67 Reconstituted ComplexPMID:15990877
forms complex withrae1RNA export factor, nucleoporin Rae1 Reconstituted ComplexPMID:15990877
binds DNA-binding domain construct withclr4histone H3 lysine methyltransferase Clr4 Two-hybridPMID:21436456
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC1D7.04 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withtco89TORC1 subunit Tco89 Negative GeneticPMID:22681890
negative genetic interaction withalm1medial ring protein Alm1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC1271.14glutamate N-acetyltransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
negative genetic interaction withtsf1mitochondrial translation elongation factor EF-Ts Tsf1 Negative GeneticPMID:22681890
negative genetic interaction withmrpl1mitochondrial ribosomal protein subunit L1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC365.16conserved protein Negative GeneticPMID:22681890
negative genetic interaction withlys4homocitrate synthase Negative GeneticPMID:22681890
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:21504829
negative genetic interaction witharg3ornithine carbamoyltransferase Arg3 Negative GeneticPMID:22681890
negative genetic interaction witharg6acetylglutamate synthase Arg6 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction witharg12argininosuccinate synthase Arg12 Negative GeneticPMID:22681890
negative genetic interaction withppr8mitochondrial PPR repeat protein Ppr8 Negative GeneticPMID:22681890
negative genetic interaction withlys2homoaconitate hydratase Lys2 Negative GeneticPMID:22681890
negative genetic interaction withSPCC1393.08transcription factor, zf-GATA type (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharg11N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Negative GeneticPMID:22681890
positive genetic interaction withnab3poly(A) binding protein Nab3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withshf1small histone ubiquitination factor Shf1 Positive GeneticPMID:22681890
positive genetic interaction withatp10mitochondrial F1-F0 ATPase assembly protein (predicted) Positive GeneticPMID:22681890
enhances phenotype ofclr3histone deacetylase (class II) Clr3 Phenotypic EnhancementPMID:21892171
rescuesclr3histone deacetylase (class II) Clr3 Phenotypic SuppressionPMID:21892171
rescuesdcr1dicer Phenotypic SuppressionPMID:21892171
rescuesago1argonaute Phenotypic SuppressionPMID:21892171
rescuesnup85nucleoporin Nup85 Phenotypic SuppressionPMID:22240020
overexpression rescuesnup85nucleoporin Nup85 Dosage RescuePMID:22240020
growth defect in presence of overexpressedred5human ZC3H3 homolog Dosage Growth DefectPMID:23658229
synthetically rescuesago1argonaute Synthetic RescuePMID:21892171
synthetically rescuesdcr1dicer Synthetic RescuePMID:21892171
synthetically rescued byrae1RNA export factor, nucleoporin Rae1 Synthetic RescuePMID:15990877
synthetic lethal withmex67mRNA export receptor, Tap, nucleoporin Mex67 Synthetic LethalityPMID:15990877
synthetic growth defect withred5human ZC3H3 homolog Synthetic Growth DefectPMID:23658229
External References
Database Identifier Description
NBRP SPBC1D7.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1D7.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1D7.04 BioGRID Interaction Datasets
Expression Viewer SPBC1D7.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1D7.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1D7.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1D7.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1D7.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1D7.04 Transcriptome Viewer (Bähler Lab)
GEO SPBC1D7.04 GEO profiles
PInt SPBC1D7.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1D7.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1D7.04 Fission yeast phenotypic data & analysis
Cyclebase SPBC1D7.04.1 Cell Cycle Data
SPD / RIKEN31/31F04Orfeome Localization Data
UniProtKB/SwissProtQ09330mRNA export protein mlo3
ModBaseQ09330Database of comparative protein structure models
STRINGQ09330Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595715RNA binding protein Mlo3
RefSeq mRNANM_001021613972h- RNA binding protein Mlo3 (mlo3), mRNA

Literature for mlo3

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016