mlo3 (SPBC1D7.04)

Gene Standard Namemlo3 Characterisation Statuspublished
Systematic IDSPBC1D7.04 Feature Typeprotein coding
Synonyms Name DescriptionMissegregation and Lethal when Overexpressed
ProductRNA binding protein Mlo3 Product Size199aa, 21.79 kDa
Genomic Location Chromosome II, 1744798-1743968 (831nt); CDS:1744712-1744113 (600nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding848
Annotation ExtensionEvidenceWith/FromReference
RNA binding644
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
poly(A)+ mRNA export from nucleus17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
transcription export complex6
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth on glucose carbon sourcemlo3ΔNull154
decreased mating efficiencymlo3ΔNull247
normal growth on amphotericin Bmlo3ΔNull17
sensitive to bleomycinmlo3ΔNull50
sensitive to bortezomib256
expressivity FYPO_EXT:0000002mlo3ΔNull
sensitive to camptothecinmlo3ΔNull214
sensitive to hydroxyureamlo3ΔNull518
sensitive to methyl methanesulfonatemlo3ΔNull227
sensitive to micafungin116
expressivity FYPO_EXT:0000002mlo3ΔNull
sensitive to UV during vegetative growthmlo3ΔNull133
viable vegetative cell populationmlo3ΔNull3781
not recorded (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
increased spontaneous diploidization24
penetrance FYPO_EXT:0000003mlo3ΔNull
normal RNA level during vegetative growth91
affecting rec8mlo3ΔNull
affecting mei4mlo3ΔNull
shortened telomeres33
expressivity FYPO_EXT:0000002mlo3ΔNull
viable curved vegetative cell61
penetrance FYPO_EXT:0000001, expressivity FYPO_EXT:0000003mlo3ΔNull
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00076 Pfam IPR000504 RNA recognition motif domain 57 124 61
SM00360 SMART IPR000504 RNA recognition motif domain 56 130 68
PS50102 Prosite Profiles IPR000504 RNA recognition motif domain 55 134 72
PTHR19965 HMMPANTHER 1 199 2
PTHR19965:SF8 HMMPANTHER 1 199 1 Gene3D IPR012677 Nucleotide-binding alpha-beta plait domain 30 133 83
SSF54928 SuperFamily 16 131 78

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000302rrm RNA recognition motifTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000302

Protein Properties

Ave. residue weight 109.50 Da
Charge 19.00
Isoelectric point 10.92
Molecular weight 21.79 kDa
Number of residues 199

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS8
present during mitotic M phase, cellular response to thiabendazoleS86
present during mitotic M phaseS2
present during mitotic M phaseS195
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S2 PMID:21712547
present during mitotic M phase experimental evidence S8 PMID:21712547
present during cellular response to thiabendazole IDA S86 PMID:18257517
present during mitotic M phase experimental evidence S86 PMID:21712547
experimental evidence S195 PMID:24763107
present during mitotic M phase experimental evidence S195 PMID:21712547
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
110182during GO:0000080PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
109524during GO:0000084PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
105166during GO:0000085PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
103038during GO:0000087PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
27161.11during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
99958during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
28228.21during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
30during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
sum3translation initiation RNA helicase Sum3 Affinity Capture-MSPMID:21436456
srp2mRNA export factor Srp2 Affinity Capture-MSPMID:21436456
has1ATP-dependent RNA helicase Has1 (predicted) Affinity Capture-MSPMID:21436456
gtp1GTP binding protein Gtp1 (predicted) Affinity Capture-MSPMID:21436456
clr4histone H3 lysine methyltransferase Clr4 Biochemical ActivityPMID:21436456
Reconstituted Complex
Affinity Capture-Western
mtr4ATP-dependent RNA helicase, TRAMP complex subunit Mtr4 Affinity Capture-MSPMID:21436456
nop52nucleolar protein Nop52 family (predicted) Affinity Capture-MSPMID:21436456
mak16nuclear HMG-like acidic protein Mak16 (predicted) Affinity Capture-MSPMID:21436456
gar1snoRNP pseudouridylase box H/ACA snoRNP complex protein Gar1 Affinity Capture-MSPMID:21436456
exo2exonuclease II Exo2 Affinity Capture-MSPMID:21436456
red5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
nts1Schizosaccharomyces specific protein Affinity Capture-MSPMID:25002536
SPAC926.08cBrix domain protein Rpf2 (predicted) Affinity Capture-MSPMID:21436456
cid14poly(A) polymerase Cid14 Affinity Capture-MSPMID:21436456
Affinity Capture-Western
rpn15proteasome regulatory particle, lid subcomplex subunit Rpn15/Dss1 Reconstituted ComplexPMID:15990877
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
kri1ribosome biogenesis protein Kri1 (predicted) Affinity Capture-MSPMID:21436456
red1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
SPAC8F11.04U3 snoRNP-associated protein Cic1/Utp30 family (predicted) Affinity Capture-MSPMID:21436456
SPCC16C4.16cconserved eukaryotic protein Affinity Capture-MSPMID:21436456
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
rrs1ribosome biogenesis protein Rrs1 (predicted) Affinity Capture-MSPMID:21436456
uap56ATP-dependent RNA helicase Uap56 Reconstituted ComplexPMID:15990877
rik1silencing protein Rik1 Affinity Capture-WesternPMID:21436456
SPCC126.11cRNA-binding protein, rrm type Affinity Capture-MSPMID:21436456
SPBC4F6.13cWD repeat/BOP1NT protein (predicted) Affinity Capture-MSPMID:21436456
rae1RNA export factor, nucleoporin Rae1 Reconstituted ComplexPMID:15990877
rmn1RNA-binding protein Affinity Capture-MSPMID:24713849
noc1ribosome biogenesis protein Noc1 (predicted) Affinity Capture-MSPMID:21436456
SPCC1672.07U3 snoRNP-associated protein Utp21 (predicted) Affinity Capture-MSPMID:21436456
nop12RNA-binding protein Nop12 (predicted) Affinity Capture-MSPMID:21436456
chp1chromodomain protein Chp1 Affinity Capture-WesternPMID:21436456
nog1GTP binding protein Nog1 (predicted) Affinity Capture-MSPMID:21436456
ppp1pescadillo-family BRCT domain protein Ppp1 (predicted) Affinity Capture-MSPMID:21436456
SPCC1322.10cell wall protein Pwp1 Affinity Capture-MSPMID:21436456
rnp24RNA-binding protein Rnp24 Affinity Capture-MSPMID:21436456
mtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
ebp2rRNA processing protein Ebp2 (predicted) Affinity Capture-MSPMID:21436456
air1zinc knuckle TRAMP complex subunit Air1 Affinity Capture-MSPMID:21436456
Affinity Capture-MSPMID:20403971
utp23rRNA processing protein Utp23 (predicted) Affinity Capture-MSPMID:21436456
sal3karyopherin Sal3 Affinity Capture-MSPMID:21436456
puf6Puf family RNA-binding protein Puf6 (predicted) Affinity Capture-MSPMID:21436456
sla1La protein homolog Affinity Capture-MSPMID:21436456
SPAC23G3.06U3 snoRNP protein Nop58 (predicted) Affinity Capture-MSPMID:21436456
brx1ribosome biogenesis protein Brx1 (predicted) Affinity Capture-MSPMID:21436456
upf1ATP-dependent RNA helicase Upf1 Affinity Capture-MSPMID:21436456
SPAC1142.04Noc complex subunit Noc2 family (predicted) Affinity Capture-MSPMID:21436456
SPCC306.07cU3 snoRNP-associated protein Cic1/Utp30 family (predicted) Affinity Capture-MSPMID:21436456
mex67mRNA export receptor, Tap, nucleoporin Mex67 Reconstituted ComplexPMID:15990877
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
SPBC4F6.14RNA-binding protein (predicted) Affinity Capture-MSPMID:21436456
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
tco89TORC1 subunit Tco89 Negative GeneticPMID:22681890
mrpl1mitochondrial ribosomal protein subunit L1 (predicted) Negative GeneticPMID:22681890
shf1small histone ubiquitination factor Shf1 Positive GeneticPMID:22681890
nup85nucleoporin Nup85 Phenotypic SuppressionPMID:22240020
Dosage Rescue
red5human ZC3H3 homolog Synthetic Growth DefectPMID:23658229
Dosage Growth Defect
sre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
lys2homoaconitate hydratase Lys2 Negative GeneticPMID:22681890
arg11N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Negative GeneticPMID:22681890
lys4homocitrate synthase Negative GeneticPMID:22681890
SPBC1271.14glutamate N-acetyltransferase (predicted) Negative GeneticPMID:22681890
SPCC1393.08transcription factor, zf-GATA type (predicted) Negative GeneticPMID:22681890
rae1RNA export factor, nucleoporin Rae1 Synthetic RescuePMID:15990877
tsf1mitochondrial translation elongation factor EF-Ts Tsf1 Negative GeneticPMID:22681890
arg12argininosuccinate synthase Arg12 Negative GeneticPMID:22681890
ago1argonaute Synthetic RescuePMID:21892171
Phenotypic Suppression
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
arg3ornithine carbamoyltransferase Arg3 Negative GeneticPMID:22681890
ppr8mitochondrial PPR repeat protein Ppr8 Negative GeneticPMID:22681890
nab3poly(A) binding protein Nab3 (predicted) Positive GeneticPMID:22681890
SPBC365.16conserved protein Negative GeneticPMID:22681890
SPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:22681890
arg6acetylglutamate synthase Arg6 (predicted) Negative GeneticPMID:22681890
alm1medial ring protein Alm1 Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
dcr1dicer Phenotypic SuppressionPMID:21892171
Synthetic Rescue
atp10mitochondrial F1-F0 ATPase assembly protein (predicted) Positive GeneticPMID:22681890
clr3histone deacetylase (class II) Clr3 Phenotypic SuppressionPMID:21892171
Phenotypic Enhancement
mex67mRNA export receptor, Tap, nucleoporin Mex67 Synthetic LethalityPMID:15990877
External References
Database Identifier Description
NBRP SPBC1D7.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1D7.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1D7.04 BioGRID Interaction Datasets
Expression Viewer SPBC1D7.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1D7.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1D7.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1D7.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1D7.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1D7.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1D7.04 Cell Cycle Data
GEO SPBC1D7.04 GEO profiles
PInt SPBC1D7.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC1D7.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC1D7.04 Fission yeast phenotypic data & analysis
SPD / RIKEN31/31F04Orfeome Localization Data
UniProtKB/SwissProtQ09330mRNA export protein mlo3
ModBaseQ09330Database of comparative protein structure models
STRINGQ09330Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595715RNA binding protein Mlo3
RefSeq mRNANM_001021613972h- RNA binding protein Mlo3 (mlo3), mRNA
European Nucleotide ArchiveL42551ENA EMBL mapping
European Nucleotide ArchiveCAB10980.1ENA Protein Mapping
UniParcUPI000012F203UniProt Archive

Literature for mlo3

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015