mlo3 (SPBC1D7.04)

Gene Standard Namemlo3 ChromosomeII
Systematic IDSPBC1D7.04 Gene Start1744798
Synonyms Gene End1743968
ProductRNA binding protein Mlo3 Gene Length831
Feature Typeprotein coding CDS Start1744712
Name DescriptionMissegregation and Lethal when Overexpressed CDS End1744113
Characterisation Statuspublished CDS Length600

Ensembl Gene Location
  1. ebs1
  2. SPNCRNA.1458
  3. mrpl7
  4. byr2
  5. SPNCRNA.1459
  6. mlo3
  7. mug80
  8. SPNCRNA.116
  9. SPNCRNA.1460
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000266sensitive to DNA damaging agentscompetitive growth assay evidencemlo3delta (deletion)deletionPMID:20537132
FYPO:0000268sensitive to UVcompetitive growth assay evidencemlo3delta (deletion)deletionPMID:20537132
FYPO:0002060viable vegetative cell populationno_name (unrecorded)unrecordedPMID:15990877
Microscopymlo3delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0003723RNA bindingISOSGD:S000002789GO_REF:0000024
GO:0005515protein bindingIPIuap56PMID:15990877
IPImex67PMID:15990877
IPIdss1PMID:15990877
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0016973poly(A)+ mRNA export from nucleusIGIrae1PMID:15990877
IMPrae1PMID:15990877
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0000346transcription export complexISOSGD:S000002789GO_REF:0000024
GO:0005634nucleusIDAPMID:16823372
IDAPMID:15990877
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
117447981743968

References

Region Start End Reference
five_prime_UTR17447981744713PMID:21511999
five_prime_UTR17447981744713PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00076 IPR000504 Pfam RNA recognition motif domain 57 124 61
SM00360 IPR000504 SMART RNA recognition motif domain 56 130 69
PS50102 IPR000504 Prosite Profiles RNA recognition motif domain 55 134 72
G3DSA:3.30.70.330 IPR012677 Gene3D 30 133 82
SSF54928 SuperFamily 16 131 78
PTHR19965 hmmpanther 19 197 2

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:18257517

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0000302rrm RNA recognition motifTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000302

Protein Properties

Ave. residue weight 109.50 Da
Charge 19.00
Isoelectric point 10.92
Molecular weight 21.79 kDa
Number of residues 199
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide28228.21PMID:23101633
experimental evidencePECO:0000014,
PECO:0000005		population_wide27161.11PMID:23101633
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide5PMID:23101633
experimental evidencePECO:0000014,
PECO:0000005		population_wide30PMID:23101633
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
shf1small histone ubiquitination factor Shf1 Positive GeneticPMID:22681890
rae1RNA export factor, nucleoporin Rae1 Synthetic RescuePMID:15990877
nup85nucleoporin Nup85 Phenotypic SuppressionPMID:22240020
Dosage Rescue
arg11N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Negative GeneticPMID:22681890
mex67mRNA export receptor, Tap, nucleoporin Mex67 Synthetic LethalityPMID:15990877
ppr8mitochondrial PPR repeat protein Ppr8 Negative GeneticPMID:22681890
sre2membrane-tethered transcription factor (predicted) Negative GeneticPMID:21504829
lys4homocitrate synthase Negative GeneticPMID:22681890
SPBC1271.14glutamate N-acetyltransferase (predicted) Negative GeneticPMID:22681890
tsf1mitochondrial translation elongation factor EF-Ts Tsf1 Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
lys2homoaconitate hydratase Lys2 Negative GeneticPMID:22681890
arg3ornithine carbamoyltransferase Arg3 Negative GeneticPMID:22681890
atp10F1-F0 ATPase assembly protein (predicted) Positive GeneticPMID:22681890
clr3histone deacetylase (class II) Clr3 Phenotypic EnhancementPMID:21892171
Phenotypic Suppression
ago1argonaute Synthetic RescuePMID:21892171
Phenotypic Suppression
SPAC1610.02cmitochondrial ribosomal protein subunit L1 (predicted) Negative GeneticPMID:22681890
dcr1dicer Synthetic RescuePMID:21892171
Phenotypic Suppression
SPCC1393.08transcription factor, zf-GATA type (predicted) Negative GeneticPMID:22681890
SPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:22681890
nab3poly(A) binding protein Nab3 (predicted) Positive GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
arg6acetylglutamate synthase Arg6 (predicted) Negative GeneticPMID:22681890
SPBC365.16conserved protein Negative GeneticPMID:22681890
arg12argininosuccinate synthase (predicted) Negative GeneticPMID:22681890
alm1medial ring protein Alm1 Negative GeneticPMID:22681890
tco89TORC1 subunit Tco89 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
air1zinc knuckle TRAMP complex subunit Air1 Affinity Capture-MSPMID:20403971
Affinity Capture-MSPMID:21436456
SPAC1142.04Noc complex subunit Noc2 family (predicted) Affinity Capture-MSPMID:21436456
clr4histone H3 lysine methyltransferase Clr4 Biochemical ActivityPMID:21436456
Reconstituted Complex
Affinity Capture-Western
Two-hybrid
ppp1pescadillo-family BRCT domain protein (predicted) Affinity Capture-MSPMID:21436456
mex67mRNA export receptor, Tap, nucleoporin Mex67 Reconstituted ComplexPMID:15990877
mtr4ATP-dependent RNA helicase, TRAMP complex subunit Mtr4 Affinity Capture-MSPMID:21436456
nop52nucleolar protein Nop52 family (predicted) Affinity Capture-MSPMID:21436456
rrs1ribosome biogenesis protein Rrs1 (predicted) Affinity Capture-MSPMID:21436456
SPBC4F6.14RNA-binding protein (predicted) Affinity Capture-MSPMID:21436456
SPAC4F10.09cribosome biogenesis protein Noc1 (predicted) Affinity Capture-MSPMID:21436456
sum3translation initiation RNA helicase Sum3 Affinity Capture-MSPMID:21436456
rnp24RNA-binding protein Rnp24 Affinity Capture-MSPMID:21436456
SPCC1322.10cell wall protein Pwp1 Affinity Capture-MSPMID:21436456
gtp1GTP binding protein Gtp1 (predicted) Affinity Capture-MSPMID:21436456
has1ATP-dependent RNA helicase Has1 (predicted) Affinity Capture-MSPMID:21436456
brx1ribosome biogenesis protein Brx1 (predicted) Affinity Capture-MSPMID:21436456
SPCC126.11cRNA-binding protein, rrm type Affinity Capture-MSPMID:21436456
mak16nuclear HMG-like acidic protein Mak16 (predicted) Affinity Capture-MSPMID:21436456
sla1La protein homolog Affinity Capture-MSPMID:21436456
exo2exonuclease II Exo2 Affinity Capture-MSPMID:21436456
uap56ATP-dependent RNA helicase Uap56 Reconstituted ComplexPMID:15990877
puf6Puf family RNA-binding protein Puf6 (predicted) Affinity Capture-MSPMID:21436456
SPCC306.07cU3 snoRNP-associated protein Cic1/Utp30 family (predicted) Affinity Capture-MSPMID:21436456
nog1GTP binding protein Nog1 (predicted) Affinity Capture-MSPMID:21436456
srp2mRNA export factor Srp2 Affinity Capture-MSPMID:21436456
rae1RNA export factor, nucleoporin Rae1 Reconstituted ComplexPMID:15990877
ebp2rRNA processing protein Ebp2 (predicted) Affinity Capture-MSPMID:21436456
nop12RNA-binding protein Nop12 (predicted) Affinity Capture-MSPMID:21436456
SPAC23G3.06U3 snoRNP protein Nop58 (predicted) Affinity Capture-MSPMID:21436456
cid14poly(A) polymerase Cid14 Affinity Capture-MSPMID:21436456
Affinity Capture-Western
SPBC4F6.13cWD repeat/BOP1NT protein (predicted) Affinity Capture-MSPMID:21436456
SPCC16C4.16cconserved eukaryotic protein Affinity Capture-MSPMID:21436456
utp23rRNA processing protein Utp23 (predicted) Affinity Capture-MSPMID:21436456
SPAC926.08cBrix domain protein Rpf2 (predicted) Affinity Capture-MSPMID:21436456
upf1ATP-dependent RNA helicase Upf1 Affinity Capture-MSPMID:21436456
chp1chromodomain protein Chp1 Affinity Capture-WesternPMID:21436456
sal3karyopherin Sal3 Affinity Capture-MSPMID:21436456
rik1silencing protein Rik1 Affinity Capture-WesternPMID:21436456
SPCC1672.07U3 snoRNP-associated protein Utp21 (predicted) Affinity Capture-MSPMID:21436456
SPAC22G7.05krr family protein (predicted) Affinity Capture-MSPMID:21436456
gar1snoRNP pseudouridylase box H/ACA snoRNP complex protein Gar1 Affinity Capture-MSPMID:21436456
dss1mRNA export protein Dss1 Reconstituted ComplexPMID:15990877
SPAC8F11.04U3 snoRNP-associated protein Cic1/Utp30 family (predicted) Affinity Capture-MSPMID:21436456
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External References
Database Identifier Description
NBRP SPBC1D7.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1D7.04 Retrieval of eukaryotic orthologs
BioGrid SPBC1D7.04 BioGRID Interaction Datasets
Bähler Lab SPBC1D7.04 Cell Cycle Expression Profile
Bähler Lab SPBC1D7.04 Meiosis/Sporulation Expression Profies
Bähler Lab SPBC1D7.04 Pheromone response/mating expression profiles
Bähler Lab SPBC1D7.04 Environmental stress expression profiles
Bähler Lab SPBC1D7.04 Bähler Lab Transcriptome Viewer
Cyclebase SPBC1D7.04 Cell Cycle Data
PInt SPBC1D7.04 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPBC1D7.04 Entrez Gene
EntrezGene2540565RNA annealing factor Mlo3
Uniprot_genenameMLO3
WikiGene2540565RNA annealing factor Mlo3
UniProtKB/SwissProtQ09330mRNA export protein mlo3
ModBaseQ09330Database of comparative protein structure models
Pfam Protein DomainsQ09330Pfam Domain Arrangement
RefSeq PeptideNP_595715RNA annealing factor Mlo3
RefSeq mRNANM_001021613972h- RNA annealing factor Mlo3 (mlo3), mRNA
European Nucleotide ArchiveAAB41270ENA Protein Mapping
European Nucleotide ArchiveCAB10980ENA Protein Mapping
SPD / RIKEN31/31F04Orfeome Localization Data
UniParcUPI000012F203UniProt Archive

Literature for mlo3
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013