mlo3 (SPBC1D7.04)


Gene Standard Namemlo3 Characterisation Statuspublished
Systematic IDSPBC1D7.04 Feature Typeprotein coding
Synonyms Name DescriptionMissegregation and Lethal when Overexpressed
ProductRNA binding protein Mlo3 Product Size199aa, 21.79 kDa
Genomic Location Chromosome II, 1744798-1743968 (831nt); CDS:1744712-1744113 (600nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003723RNA bindingISOSGD:S000002789GO_REF:0000024628
GO:0005515protein bindingIPIrpn15PMID:15990877861
IPImex67PMID:15990877
IPIuap56PMID:15990877
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016973poly(A)+ mRNA export from nucleusIMPrae1PMID:1599087717
IGIrae1PMID:15990877
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005634nucleusIDAPMID:159908772740
IDAPMID:16823372
GO:0000346transcription export complexISOSGD:S000002789GO_REF:00000246
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaymlo3ΔNullPECO:0000126, PECO:0000102, PECO:0000005PMID:2365822999
FYPO:0000980normal growth on amphotericin BCell growth assaymlo3ΔNullPECO:0000137, PECO:0000005PMID:2373802117
FYPO:0000268sensitive to UV during vegetative growthcompetitive growth assay evidencemlo3ΔNullPMID:20537132117
Cell growth assaymlo3ΔNullPECO:0000137, PECO:0000005PMID:23173672
FYPO:0000095sensitive to bleomycinCell growth assaymlo3ΔNullPECO:0000137, PECO:0000005PMID:2317367247
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000002Cell growth assaymlo3ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0000085sensitive to camptothecinCell growth assaymlo3ΔNullPECO:0000137, PECO:0000005PMID:23173672211
FYPO:0000088sensitive to hydroxyureaCell growth assaymlo3ΔNullPECO:0000137, PECO:0000005PMID:23173672506
FYPO:0000089sensitive to methyl methanesulfonateCell growth assaymlo3ΔNullPECO:0000137, PECO:0000005PMID:23173672210
FYPO:0002641sensitive to micafungin108
expressivity FYPO_EXT:0000002Cell growth assaymlo3ΔNullPECO:0000137, PECO:0000005PMID:23738021
FYPO:0002060viable vegetative cell populationMicroscopymlo3ΔNullPMID:204732893760
Microscopymlo3ΔNullPECO:0000004, PECO:0000137PMID:23697806
not recorded (unrecorded)PMID:15990877

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencemlo3ΔNullPECO:0000015PMID:23950735230
FYPO:0000252increased spontaneous diploidization21
penetrance FYPO_EXT:0000003Flow cytometry datamlo3ΔNullPECO:0000137, PECO:0000005PMID:23173672
FYPO:0001317normal RNA level during vegetative growth56
affecting mei4Transcript expression level evidencemlo3ΔNullPMID:23658229
affecting rec8Transcript expression level evidencemlo3ΔNullPMID:23658229
FYPO:0002112viable curved vegetative cell57
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopymlo3ΔNullPECO:0000137, PECO:0000004PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
117447981743968

UTRs

Region Coordinates Reference
five_prime_UTR1744798..1744713PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00076 Pfam IPR000504 RNA recognition motif domain 57 124 61
SM00360 SMART IPR000504 RNA recognition motif domain 56 130 69
PS50102 Prosite Profiles IPR000504 RNA recognition motif domain 55 134 72
PTHR19965 HMMPANTHER 1 199 2
PTHR19965:SF8 HMMPANTHER 1 199 1
3.30.70.330 Gene3D IPR012677 Nucleotide-binding, alpha-beta plait 30 133 82
SSF54928 SuperFamily 16 131 78

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000302rrm RNA recognition motifTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000302

Protein Properties

Ave. residue weight 109.50 Da
Charge 19.00
Isoelectric point 10.92
Molecular weight 21.79 kDa
Number of residues 199
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS195PMID:217125471663
present during mitotic M phase
present during mitotic M phaseS8PMID:21712547
present during cellular response to thiabendazoleIDAS86PMID:18257517
present during mitotic M phaseS2PMID:21712547
present during mitotic M phaseS86PMID:21712547
S195PMID:24763107
MOD:00696phosphorylated residueNASPMID:182575171915
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
28228.21during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
99958during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
27161.11during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
110182during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
109524during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
105166during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
103038during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
30during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
tco89TORC1 subunit Tco89 Negative GeneticPMID:22681890
SPAC1610.02cmitochondrial ribosomal protein subunit L1 (predicted) Negative GeneticPMID:22681890
shf1small histone ubiquitination factor Shf1 Positive GeneticPMID:22681890
nup85nucleoporin Nup85 Phenotypic SuppressionPMID:22240020
Dosage Rescue
red5human ZC3H3 homolog Synthetic Growth DefectPMID:23658229
Dosage Growth Defect
sre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
lys2homoaconitate hydratase Lys2 Negative GeneticPMID:22681890
arg11N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Negative GeneticPMID:22681890
lys4homocitrate synthase Negative GeneticPMID:22681890
SPBC1271.14glutamate N-acetyltransferase (predicted) Negative GeneticPMID:22681890
SPCC1393.08transcription factor, zf-GATA type (predicted) Negative GeneticPMID:22681890
rae1RNA export factor, nucleoporin Rae1 Synthetic RescuePMID:15990877
tsf1mitochondrial translation elongation factor EF-Ts Tsf1 Negative GeneticPMID:22681890
arg12argininosuccinate synthase Arg12 Negative GeneticPMID:22681890
ago1argonaute Synthetic RescuePMID:21892171
Phenotypic Suppression
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
arg3ornithine carbamoyltransferase Arg3 Negative GeneticPMID:22681890
ppr8mitochondrial PPR repeat protein Ppr8 Negative GeneticPMID:22681890
nab3poly(A) binding protein Nab3 (predicted) Positive GeneticPMID:22681890
SPBC365.16conserved protein Negative GeneticPMID:22681890
SPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:22681890
arg6acetylglutamate synthase Arg6 (predicted) Negative GeneticPMID:22681890
alm1medial ring protein Alm1 Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
dcr1dicer Phenotypic SuppressionPMID:21892171
Synthetic Rescue
atp10F1-F0 ATPase assembly protein (predicted) Positive GeneticPMID:22681890
clr3histone deacetylase (class II) Clr3 Phenotypic EnhancementPMID:21892171
Phenotypic Suppression
mex67mRNA export receptor, Tap, nucleoporin Mex67 Synthetic LethalityPMID:15990877
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
sum3translation initiation RNA helicase Sum3 Affinity Capture-MSPMID:21436456
srp2mRNA export factor Srp2 Affinity Capture-MSPMID:21436456
has1ATP-dependent RNA helicase Has1 (predicted) Affinity Capture-MSPMID:21436456
gtp1GTP binding protein Gtp1 (predicted) Affinity Capture-MSPMID:21436456
clr4histone H3 lysine methyltransferase Clr4 Reconstituted ComplexPMID:21436456
Two-hybrid
Affinity Capture-Western
Biochemical Activity
mtr4ATP-dependent RNA helicase, TRAMP complex subunit Mtr4 Affinity Capture-MSPMID:21436456
nop52nucleolar protein Nop52 family (predicted) Affinity Capture-MSPMID:21436456
mak16nuclear HMG-like acidic protein Mak16 (predicted) Affinity Capture-MSPMID:21436456
gar1snoRNP pseudouridylase box H/ACA snoRNP complex protein Gar1 Affinity Capture-MSPMID:21436456
exo2exonuclease II Exo2 Affinity Capture-MSPMID:21436456
SPAC926.08cBrix domain protein Rpf2 (predicted) Affinity Capture-MSPMID:21436456
cid14poly(A) polymerase Cid14 Affinity Capture-MSPMID:21436456
Affinity Capture-Western
rpn15proteasome regulatory particle, lid subcomplex subunit Rpn15/Dss11 Reconstituted ComplexPMID:15990877
SPAC22G7.05krr family protein (predicted) Affinity Capture-MSPMID:21436456
SPAC8F11.04U3 snoRNP-associated protein Cic1/Utp30 family (predicted) Affinity Capture-MSPMID:21436456
SPCC16C4.16cconserved eukaryotic protein Affinity Capture-MSPMID:21436456
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
rrs1ribosome biogenesis protein Rrs1 (predicted) Affinity Capture-MSPMID:21436456
uap56ATP-dependent RNA helicase Uap56 Reconstituted ComplexPMID:15990877
rik1silencing protein Rik1 Affinity Capture-WesternPMID:21436456
SPCC126.11cRNA-binding protein, rrm type Affinity Capture-MSPMID:21436456
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
SPBC4F6.13cWD repeat/BOP1NT protein (predicted) Affinity Capture-MSPMID:21436456
rae1RNA export factor, nucleoporin Rae1 Reconstituted ComplexPMID:15990877
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
SPAC4F10.09cribosome biogenesis protein Noc1 (predicted) Affinity Capture-MSPMID:21436456
SPCC1672.07U3 snoRNP-associated protein Utp21 (predicted) Affinity Capture-MSPMID:21436456
nop12RNA-binding protein Nop12 (predicted) Affinity Capture-MSPMID:21436456
chp1chromodomain protein Chp1 Affinity Capture-WesternPMID:21436456
nog1GTP binding protein Nog1 (predicted) Affinity Capture-MSPMID:21436456
ppp1pescadillo-family BRCT domain protein Ppp1 (predicted) Affinity Capture-MSPMID:21436456
SPCC1322.10cell wall protein Pwp1 Affinity Capture-MSPMID:21436456
rnp24RNA-binding protein Rnp24 Affinity Capture-MSPMID:21436456
ebp2rRNA processing protein Ebp2 (predicted) Affinity Capture-MSPMID:21436456
air1zinc knuckle TRAMP complex subunit Air1 Affinity Capture-MSPMID:21436456
Affinity Capture-MSPMID:20403971
utp23rRNA processing protein Utp23 (predicted) Affinity Capture-MSPMID:21436456
sal3karyopherin Sal3 Affinity Capture-MSPMID:21436456
puf6Puf family RNA-binding protein Puf6 (predicted) Affinity Capture-MSPMID:21436456
sla1La protein homolog Affinity Capture-MSPMID:21436456
SPAC23G3.06U3 snoRNP protein Nop58 (predicted) Affinity Capture-MSPMID:21436456
brx1ribosome biogenesis protein Brx1 (predicted) Affinity Capture-MSPMID:21436456
upf1ATP-dependent RNA helicase Upf1 Affinity Capture-MSPMID:21436456
SPAC1142.04Noc complex subunit Noc2 family (predicted) Affinity Capture-MSPMID:21436456
SPCC306.07cU3 snoRNP-associated protein Cic1/Utp30 family (predicted) Affinity Capture-MSPMID:21436456
mex67mRNA export receptor, Tap, nucleoporin Mex67 Reconstituted ComplexPMID:15990877
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
SPBC4F6.14RNA-binding protein (predicted) Affinity Capture-MSPMID:21436456
External References
Database Identifier Description
NBRP SPBC1D7.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC1D7.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC1D7.04 BioGRID Interaction Datasets
Expression Viewer SPBC1D7.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC1D7.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC1D7.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC1D7.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC1D7.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC1D7.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC1D7.04 Cell Cycle Data
GEO SPBC1D7.04 GEO profiles
PInt SPBC1D7.04 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2540565RNA annealing factor Mlo3
WikiGene2540565RNA annealing factor Mlo3
SPD / RIKEN31/31F04Orfeome Localization Data
UniProtKB/SwissProtQ09330mRNA export protein mlo3
ModBaseQ09330Database of comparative protein structure models
STRINGQ09330Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595715RNA annealing factor Mlo3
RefSeq mRNANM_001021613972h- RNA annealing factor Mlo3 (mlo3), mRNA
European Nucleotide ArchiveL42551ENA EMBL mapping
European Nucleotide ArchiveAAB41270ENA Protein Mapping
European Nucleotide ArchiveCAB10980ENA Protein Mapping
European Nucleotide ArchiveCAB10980.1ENA Protein Mapping
UniParcUPI000012F203UniProt Archive

Literature for mlo3

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014