med8 (SPBC21.04)

Gene Standard Namemed8 Characterisation Statuspublished
Systematic IDSPBC21.04 Feature Typeprotein coding
Synonymssep15 Name Description
Productmediator complex subunit Med8 Product Size200aa, 23.33 kDa
Genomic Location Chromosome II, 3219316-3222152 (2837nt); CDS:3219604-3220343 (740nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding866
Annotation ExtensionEvidenceWith/FromReference
RNA polymerase II transcription cofactor activity18
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular protein localization519
Annotation ExtensionEvidenceWith/FromReference
regulation of transcription from RNA polymerase II promoter296
Annotation ExtensionEvidenceWith/FromReference
transcription from RNA polymerase II promoter340
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
mediator complex22
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationmed8Δ1443
sensitive to caffeine during vegetative growthmed8-ts106
sensitive to zincmed8-ts5

Cell Phenotype

Term NameGenotypesCount
abnormal septation160
elongated multiseptate vegetative cellsep15-59856
inviable curved septated vegetative cellmed8Δ10
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3219316..3219731, 3219869..3222152
5' UTR3219316..3219603PMID:21511999
CDS3219604..3219731, 3219869..3220343
3' UTR3220344..3222152PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10232 Pfam IPR019364 Mediator complex, subunit Med8, fungi/metazoa 6 149 1
PTHR13074 HMMPANTHER 4 173 1
PTHR13074:SF9 HMMPANTHER 4 173 1
PD114219 blastprodom IPR020178 Mediator complex, subunit Med8, fungi 11 199 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.67 Da
Charge -7.50
Codon Adpatation Index 0.36
Isoelectric point 4.88
Molecular weight 23.33 kDa
Number of residues 200
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
303.7during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
7859.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.2during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
deletion mutant expression profiling PMID:15829570 PMID:1792223677
Taxonomic Conservation
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3421
conserved in vertebrates3396

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byace2transcription factor Ace2 Affinity Capture-WesternPMID:19720063
affinity captured bymed13mediator complex subunit Med13 Affinity Capture-MSPMID:12738880
co-purifies withmed18mediator complex subunit Med18 Co-purificationPMID:18310102
forms complex withmed18mediator complex subunit Med18 Reconstituted ComplexPMID:18381891
Co-crystal or NMR structuremed18mediator complex subunit Med18 Co-crystal or NMR structurePMID:18381891
co-purifies withmed20mediator complex subunit Med20 Co-purificationPMID:18310102
affinity captured bymed7mediator complex subunit Med7 Affinity Capture-MSPMID:15182371
co-purifies withpmc2mediator complex subunit Med1 Co-purificationPMID:18310102
co-purifies withpmc3mediator complex subunit Med27 Co-purificationPMID:18310102
binds DNA-binding domain construct withrpb4DNA-directed RNA polymerase II complex subunit Rpb4 Two-hybridPMID:19720063
forms complex withrpb4DNA-directed RNA polymerase II complex subunit Rpb4 Reconstituted ComplexPMID:19720063
co-purifies withsrb4mediator complex subunit Med17 Co-purificationPMID:18310102
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withcdc16two-component GAP Cdc16 Synthetic Growth DefectPMID:10392445
synthetic lethal withmed31mediator complex subunit Med31 Synthetic LethalityPMID:17922236
synthetic lethal withsep1forkhead transcription factor Sep1 Synthetic LethalityPMID:10392445
phenotype enhanced bywee1M phase inhibitor protein kinase Wee1 Phenotypic EnhancementPMID:10392445
External References
Database Identifier Description
NBRP SPBC21.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC21.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC21.04 BioGRID Interaction Datasets
Expression Viewer SPBC21.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC21.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC21.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC21.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC21.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC21.04 Transcriptome Viewer (Bähler Lab)
GEO SPBC21.04 GEO profiles
PInt SPBC21.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC21.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC21.04 Fission yeast phenotypic data & analysis
Cyclebase SPBC21.04.1 Cell Cycle Data
SPD / RIKEN43/43A10Orfeome Localization Data
UniProtKB/SwissProtO94646Mediator of RNA polymerase II transcription subunit 8
ModBaseO94646Database of comparative protein structure models
STRINGO94646Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596338mediator complex subunit Med8
RefSeq mRNANM_001022259972h- mediator complex subunit Med8 (med8), mRNA
European Nucleotide ArchiveAF093755ENA EMBL mapping
European Nucleotide ArchiveCU329671ENA EMBL mapping
ePDB3C0TThe European PDB
ePDB4H63The European PDB
European Nucleotide Archive-ENA Protein Mapping
European Nucleotide ArchiveCAB36884ENA Protein Mapping
European Nucleotide ArchiveCAB36884.1ENA Protein Mapping
UniParcUPI0000069B4AUniProt Archive

Literature for med8

Search: Europe PMC or PubMed

Release Version: PomBase:28_55 - 09 Sep 2015