med8 (SPBC21.04)


Gene Standard Namemed8 Characterisation Statuspublished
Systematic IDSPBC21.04 Feature Typeprotein coding
Synonymssep15 Name Description
Productmediator complex subunit Med8 Product Size200aa, 23.33 kDa
Genomic Location Chromosome II, 3219316-3222152 (2837nt); CDS:3219604-3220343 (740nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0001104RNA polymerase II transcription cofactor activityIEAIPR019364GO_REF:000000218
GO:0005515protein bindingIPImed20PMID:18310102861
residue 107-200IPIrpb4PMID:19720063
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034613cellular protein localization554
localizes med20IMPPMID:18310102
localizes pmc3IMPPMID:18310102
localizes sep11IMPPMID:18310102
GO:1900405regulation of cytokinetic cell separation by regulation of transcription from RNA polymerase II promoterIMPPMID:179222363
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0016592mediator complexIDAPMID:1157293922
IDAPMID:10625684
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationmed8ΔNullPMID:111912051315
Microscopymed8ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopymed8ΔNullPMID:20473289
FYPO:0000097sensitive to caffeinemed8-tsPMID:1972006398
FYPO:0000116sensitive to zincmed8-tsPMID:197200633

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000117abnormal septationnot recorded (unrecorded)PMID:1831010240
med8-tsPMID:19720063
sep15-598PMID:11191205
FYPO:0000223elongated multiseptate cellsep15-598PMID:1119120540
FYPO:0002282inviable curved septated vegetative cell10
penetrance FYPO_EXT:0000001Microscopymed8ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
132193163219731
232198693222152

UTRs

Region Coordinates Reference
five_prime_UTR3219316..3219603PMID:21511999
three_prime_UTR3220344..3222152PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10232 Pfam IPR019364 Mediator complex, subunit Med8, fungi/metazoa 1 189 1
PTHR13074 HMMPANTHER 4 173 1
PTHR13074:SF9 HMMPANTHER 4 173 1
PD114219 blastprodom IPR020178 Mediator complex, subunit Med8, fungi 11 199 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.67 Da
Charge -7.50
Isoelectric point 4.88
Molecular weight 23.33 kDa
Number of residues 200
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
303.7during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
7859.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
3.2during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:15829570 PMID:1792223679
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
med31mediator complex subunit Med31 Synthetic LethalityPMID:17922236
cdc16two-component GAP Cdc16 Synthetic Growth DefectPMID:10392445
sep1forkhead transcription factor Sep1 Synthetic LethalityPMID:10392445
wee1M phase inhibitor protein kinase Wee1 Phenotypic EnhancementPMID:10392445
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
med20mediator complex subunit Med20 Co-purificationPMID:18310102
med7mediator complex subunit Med7 Affinity Capture-MSPMID:15182371
pmc2mediator complex subunit Pmc2/Med1 Co-purificationPMID:18310102
srb4mediator complex subunit Srb4 Co-purificationPMID:18310102
ace2transcription factor Ace2 Affinity Capture-WesternPMID:19720063
med13mediator complex subunit Srb9 Affinity Capture-MSPMID:12738880
sep11mediator complex subunit Pmc6 Co-purificationPMID:18310102
Reconstituted ComplexPMID:18381891
Co-crystal or NMR structure
pmc3mediator complex subunit Pmc3/Med27 Co-purificationPMID:18310102
rpb4DNA-directed RNA polymerase II complex subunit Rpb4 Two-hybridPMID:19720063
Reconstituted Complex
External References
Database Identifier Description
NBRP SPBC21.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC21.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC21.04 BioGRID Interaction Datasets
Expression Viewer SPBC21.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC21.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC21.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC21.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC21.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC21.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC21.04 Cell Cycle Data
GEO SPBC21.04 GEO profiles
PInt SPBC21.04 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2540761mediator complex subunit Med8
WikiGene2540761mediator complex subunit Med8
SPD / RIKEN43/43A10Orfeome Localization Data
UniProtKB/SwissProtO94646Mediator of RNA polymerase II transcription subunit 8
ModBaseO94646Database of comparative protein structure models
STRINGO94646Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596338mediator complex subunit Med8
RefSeq mRNANM_001022259972h- mediator complex subunit Med8 (med8), mRNA
ePDB3C0TThe European PDB
PDB3C0TPDB
PDBsum3C0TPDBsum
ePDB4H63The European PDB
PDB4H63PDB
PDBsum4H63PDBsum
European Nucleotide Archive-ENA Protein Mapping
European Nucleotide ArchiveCAB36884ENA Protein Mapping
European Nucleotide ArchiveCAB36884.1ENA Protein Mapping
UniParcUPI0000069B4AUniProt Archive

Literature for med8

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014