ral2 (SPBC21.05c)


Gene Standard Nameral2 Characterisation Statuspublished
Systematic IDSPBC21.05c Feature Typeprotein coding
Synonyms Name DescriptionRAs Like
ProductRas1-Scd pathway protein Ral2 Product Size611aa, 69.85 kDa
Genomic Location Chromosome II, 3222399-3219942 (2458nt); CDS:3222181-3220346 (1836nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
Cdc42 protein signal transduction7
Annotation ExtensionEvidenceWith/FromReference
conjugation with cellular fusion114
Annotation ExtensionEvidenceWith/FromReference
positive regulation of cell-substrate adhesion6
Annotation ExtensionEvidenceWith/FromReference
regulation of filamentous growth10
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in conjugation with cellular fusion29
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4211
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyral2272
decreased sporulation frequencyhomozygous diploid (ral2mutant/ral2mutant)103
viable vegetative cell populationral2Δ3840

Cell Phenotype

Term NameGenotypesCount
pseudohyphal growth abolishedral2Δ12
spheroid cell during nitrogen starvationral2Δ3
sterileral2mutant61
23A
ral2Δ
stubby cellral2mutant107
viable spheroid vegetative cellral2Δ52
viable stubby vegetative cellral2Δ75
viable vegetative cell with abnormal cell shaperal2Δ700

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased mating efficiencyras1-val17 (G17V), ral2Δ

Cell Phenotype

Term NameGenotypes
normal sporulationras1-val17 (G17V), ral2Δ
viable vegetative cell with normal cell morphologyras1-val17 (G17V), ral2Δ
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3222399..3219942
mRNA3222399..3219942
5' UTR3222399..3222182PMID:21511999
CDS3222181..3220346
3' UTR3220345..3219942PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13415 Pfam 41 90 2
PTHR23244 HMMPANTHER 308 380 3
PTHR23244 HMMPANTHER 154 210 3
PTHR23244 HMMPANTHER 25 132 3
1zgkA00 Gene3D IPR015915 Kelch-type beta propeller 26 204 5
1zgkA00 Gene3D IPR015915 Kelch-type beta propeller 305 385 5
0052715 SuperFamily 24 134 3
0052715 SuperFamily 161 202 3
0052715 SuperFamily 302 382 3

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001445BTB/POZ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001445
PBO:0001938kelch repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001938

Protein Properties

Ave. residue weight 114.32 Da
Charge 0.00
Codon Adaptation Index 0.37
Isoelectric point 6.51
Molecular weight 69.85 kDa
Number of residues 611
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ral2 (SPBC21.05c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
468.59during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.43during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
Ras1-Scd10
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only618
conserved in fungi4606
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC21.05c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withskp1SCF ubiquitin ligase complex subunit Skp1 Two-hybridPMID:17016471
binds DNA-binding domain construct withgef1Cdc42 RhoGEF Gef1 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC21.05c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withfan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
rescued byras1GTPase Ras1 Phenotypic SuppressionPMID:3071741
rescuesscd1RhoGEF Scd1 Phenotypic SuppressionPMID:3071741
External References
Database Identifier Description
NBRP SPBC21.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC21.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC21.05c BioGRID Interaction Datasets
Expression Viewer SPBC21.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC21.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC21.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC21.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC21.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC21.05c Transcriptome Viewer (Bähler Lab)
GEO SPBC21.05c GEO profiles
PInt SPBC21.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC21.05c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC21.05c Fission yeast phenotypic data & analysis
Cyclebase SPBC21.05c.1 Cell Cycle Data
SPD / RIKEN33/33E05Orfeome Localization Data
UniProtKB/SwissProtP15258Protein ral2
ModBaseP15258Database of comparative protein structure models
STRINGP15258Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596339Ras1-Scd pathway protein Ral2
RefSeq mRNANM_001022260972h- Ras1-Scd pathway protein Ral2 (ral2), mRNA

Literature for ral2

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016