ral2 (SPBC21.05c)


Gene Standard Nameral2 Characterisation Statuspublished
Systematic IDSPBC21.05c Feature Typeprotein coding
Synonyms Name DescriptionRAs Like
ProductRas1-Scd pathway protein Ral2 Product Size611aa, 69.85 kDa
Genomic Location Chromosome II, 3222399-3219942 (2458nt); CDS:3222181-3220346 (1836nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTPase regulator activity68
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
Cdc42 protein signal transduction9
Annotation ExtensionEvidenceWith/FromReference
conjugation with cellular fusion110
Annotation ExtensionEvidenceWith/FromReference
positive regulation of cell-substrate adhesion7
Annotation ExtensionEvidenceWith/FromReference
regulation of filamentous growth12
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in conjugation with cellular fusion26
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4205
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum594
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyral2ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopyral2ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidenceral2NullPECO:0000015PMID:23950735240
FYPO:0000584decreased sporulationCell growth assayhomozygous diploid (ral2mutant/ral2mutant)Not specifiedPMID:307174161
FYPO:0000551pseudohyphal growth abolishedMicroscopyral2ΔNullPMID:1954231212
FYPO:0001957spheroid cell during nitrogen starvationMicroscopyral2ΔNullPMID:20383193
FYPO:0000280sterileCell growth assayral2ΔNullPMID:258652850
Cell growth assayral2mutantNot specifiedPMID:3071741
Cell growth assay23ANot specifiedPMID:8575018
Cell growth assayral2ΔNullPMID:9133664
FYPO:0000024stubby cellMicroscopyral2mutantNot specifiedPMID:307174199
FYPO:0002380viable spheroid vegetative cellMicroscopyral2ΔNullPMID:258652842
penetrance FYPO_EXT:0000001Microscopyral2ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0002106viable stubby vegetative cell69
expressivity FYPO_EXT:0000001
penetrance FYPO_EXT:0000001
experimental phenotypic evidenceral2ΔNullPECO:0000137, PECO:0000005, PECO:0000103DOI:10.1016/j.devcel.2014.09.005
Microscopyral2ΔNullPMID:2586528
FYPO:0002197viable vegetative cell with abnormal cell shape467
penetrance FYPO_EXT:0000001experimental phenotypic evidenceral2ΔNullPECO:0000005, PECO:0000103, PECO:0000137DOI:10.1016/j.devcel.2014.09.005
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
132223993219942

UTRs

Region Coordinates Reference
five_prime_UTR3222399..3222182PMID:21511999
three_prime_UTR3220345..3219942PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13415 Pfam 41 89 2
PTHR23244:SF224 HMMPANTHER 27 217 1
PTHR23244 HMMPANTHER 27 217 3
1zgkA00 Gene3D IPR015915 Kelch-type beta propeller 305 385 5
1zgkA00 Gene3D IPR015915 Kelch-type beta propeller 26 204 5
0052715 SuperFamily 24 134 3
0052715 SuperFamily 302 382 3
0052715 SuperFamily 161 202 3

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001445BTB/POZ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001445
PBO:0001938kelch repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001938

Protein Properties

Ave. residue weight 114.32 Da
Charge 0.00
Isoelectric point 6.51
Molecular weight 69.85 kDa
Number of residues 611
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
468.59during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.43during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
Ras1-Scd10
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only623
conserved in fungi4600
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
skp1SCF ubiquitin ligase complex subunit Skp1 Two-hybridPMID:17016471
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
fan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
scd1RhoGEF Scd1 Phenotypic SuppressionPMID:3071741
ras1GTPase Ras1 Phenotypic SuppressionPMID:3071741
Dosage RescuePMID:2586528
Phenotypic Suppression
External References
Database Identifier Description
NBRP SPBC21.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC21.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC21.05c BioGRID Interaction Datasets
Expression Viewer SPBC21.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC21.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC21.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC21.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC21.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC21.05c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC21.05c Cell Cycle Data
GEO SPBC21.05c GEO profiles
PInt SPBC21.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC21.05c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC21.05c Fission yeast phenotypic data & analysis
SPD / RIKEN33/33E05Orfeome Localization Data
UniProtKB/SwissProtP15258Protein ral2
ModBaseP15258Database of comparative protein structure models
STRINGP15258Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596339Ras1-Scd pathway protein Ral2
RefSeq mRNANM_001022260972h- Ras1-Scd pathway protein Ral2 (ral2), mRNA
European Nucleotide ArchiveAAA35331.1ENA Protein Mapping
European Nucleotide ArchiveCAB36885.1ENA Protein Mapping
UniParcUPI00001330F4UniProt Archive

Literature for ral2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014