ral2 (SPBC21.05c)


Gene Standard Nameral2 Characterisation Statuspublished
Systematic IDSPBC21.05c Feature Typeprotein coding
Synonyms Name DescriptionRAs Like
ProductRas1-Scd pathway protein Ral2 Product Size611aa, 69.85 kDa
Genomic Location Chromosome II, 3222399-3219942 (2458nt); CDS:3222181-3220346 (1836nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
Cdc42 protein signal transduction7
Annotation ExtensionEvidenceWith/FromReference
conjugation with cellular fusion114
Annotation ExtensionEvidenceWith/FromReference
positive regulation of cell-substrate adhesion6
Annotation ExtensionEvidenceWith/FromReference
regulation of filamentous growth12
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in conjugation with cellular fusion29
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4211
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum595
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyral2252
decreased sporulation frequencyhomozygous diploid (ral2mutant/ral2mutant)95
viable vegetative cell populationral2Δ3819

Cell Phenotype

Term NameGenotypesCount
pseudohyphal growth abolishedral2Δ12
spheroid cell during nitrogen starvationral2Δ3
sterile23A57
ral2mutant
ral2Δ
stubby cellral2mutant104
viable spheroid vegetative cellral2Δ50
viable stubby vegetative cellral2Δ73
viable vegetative cell with abnormal cell shaperal2Δ687

Multi-allele phenotypes


Population Phenotype

Term NameGenotypes
decreased mating efficiencyral2Δ, ras1-val17 (G17V)

Cell Phenotype

Term NameGenotypes
normal sporulationral2Δ, ras1-val17 (G17V)
viable vegetative cell with normal cell morphologyral2Δ, ras1-val17 (G17V)
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3222399..3219942
mRNA3222399..3219942
5' UTR3222399..3222182PMID:21511999
CDS3222181..3220346
3' UTR3220345..3219942PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13415 Pfam 41 90 2
PTHR23244 HMMPANTHER 308 380 3
PTHR23244 HMMPANTHER 154 210 3
PTHR23244 HMMPANTHER 25 132 3
1zgkA00 Gene3D IPR015915 Kelch-type beta propeller 305 385 5
1zgkA00 Gene3D IPR015915 Kelch-type beta propeller 26 204 5
0052715 SuperFamily 302 382 3
0052715 SuperFamily 161 202 3
0052715 SuperFamily 24 134 3

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001445BTB/POZ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001445
PBO:0001938kelch repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001938

Protein Properties

Ave. residue weight 114.32 Da
Charge 0.00
Isoelectric point 6.51
Molecular weight 69.85 kDa
Number of residues 611
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
468.59during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.43during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
Ras1-Scd10
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPBC21.05c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withskp1SCF ubiquitin ligase complex subunit Skp1 Two-hybridPMID:17016471
Genetic Interactions

Source: BioGRID

Load gene that interact geneitically with SPBC21.05c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withfan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
rescuesscd1RhoGEF Scd1 Phenotypic SuppressionPMID:3071741
rescued byras1GTPase Ras1 Phenotypic SuppressionPMID:2586528
External References
Database Identifier Description
NBRP SPBC21.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC21.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC21.05c BioGRID Interaction Datasets
Expression Viewer SPBC21.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC21.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC21.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC21.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC21.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC21.05c Transcriptome Viewer (Bähler Lab)
GEO SPBC21.05c GEO profiles
PInt SPBC21.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC21.05c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC21.05c Fission yeast phenotypic data & analysis
Cyclebase SPBC21.05c.1 Cell Cycle Data
SPD / RIKEN33/33E05Orfeome Localization Data
UniProtKB/SwissProtP15258Protein ral2
ModBaseP15258Database of comparative protein structure models
STRINGP15258Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596339Ras1-Scd pathway protein Ral2
RefSeq mRNANM_001022260972h- Ras1-Scd pathway protein Ral2 (ral2), mRNA
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveM30827ENA EMBL mapping
European Nucleotide ArchiveAAA35331ENA Protein Mapping
European Nucleotide ArchiveAAA35331.1ENA Protein Mapping
European Nucleotide ArchiveCAB36885ENA Protein Mapping
European Nucleotide ArchiveCAB36885.1ENA Protein Mapping
UniParcUPI00001330F4UniProt Archive

Literature for ral2

Search: Europe PMC or PubMed

Release Version: PomBase:29_56 - 12 Nov 2015