ral2 (SPBC21.05c)


Gene Standard Nameral2 Characterisation Statuspublished
Systematic IDSPBC21.05c Feature Typeprotein coding
Synonyms Name DescriptionRAs Like
ProductRas1-Scd pathway protein Ral2 Product Size611aa, 69.85 kDa
Genomic Location Chromosome II, 3222399-3219942 (2458nt); CDS:3222181-3220346 (1836nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030695GTPase regulator activityNASGO_REF:000005167
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032488Cdc42 protein signal transductionNASGO_REF:00000019
GO:0000747conjugation with cellular fusionIMPPMID:857501862
IMPPMID:3071741
GO:0010811positive regulation of cell-substrate adhesionIMPPMID:195423127
GO:0010570regulation of filamentous growthIMPPMID:1954231213
GO:0032005signal transduction involved in conjugation with cellular fusionIGIras1PMID:258652818
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISSUniProtKB:P53094GO_REF:00000014201
GO:0005783endoplasmic reticulumIDAPMID:16823372593
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyral2ΔNullPMID:204732893730
Microscopyral2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidenceral2NullPECO:0000015PMID:23950735235
FYPO:0000584decreased sporulationCell growth assayhomozygous diploid (ral2mutant/ral2mutant)Not specifiedPMID:307174148
FYPO:0000551pseudohyphal growth abolishedMicroscopyral2ΔNullPMID:1954231212
FYPO:0001957spheroid cell during nitrogen starvationMicroscopyral2ΔNullPMID:20383193
FYPO:0000280sterileCell growth assayral2mutantNot specifiedPMID:307174147
Cell growth assayral2ΔNullPMID:2586528
FYPO:0000024stubby cellMicroscopyral2mutantNot specifiedPMID:307174184
FYPO:0002380viable spheroid vegetative cell37
penetrance FYPO_EXT:0000001Microscopyral2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopyral2ΔNullPMID:2586528
FYPO:0002106viable stubby vegetative cellMicroscopyral2ΔNullPMID:258652852
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
132223993219942

UTRs

Region Coordinates Reference
five_prime_UTR3222399..3222182PMID:21511999
three_prime_UTR3220345..3219942PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13415 Pfam 41 89 2
PTHR23244 HMMPANTHER 24 417 3
PTHR23244:SF277 HMMPANTHER 24 417 1
1zgkA00 Gene3D IPR015915 Kelch-type beta propeller 305 385 5
1zgkA00 Gene3D IPR015915 Kelch-type beta propeller 26 204 5
0052715 SuperFamily 24 134 3
0052715 SuperFamily 302 382 3
0052715 SuperFamily 161 202 3

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001445BTB/POZ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001445
PBO:0001938kelch repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001938

Protein Properties

Ave. residue weight 114.32 Da
Charge 0.00
Isoelectric point 6.51
Molecular weight 69.85 kDa
Number of residues 611
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
468.59during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.43during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
Ras1-Scd10
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi only603
conserved in fungi4569
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
fan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
scd1RhoGEF Scd1 Phenotypic SuppressionPMID:3071741
ras1GTPase Ras1 Phenotypic SuppressionPMID:3071741
Phenotypic SuppressionPMID:2586528
Dosage Rescue
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
skp1SCF ubiquitin ligase complex subunit Skp1 Two-hybridPMID:17016471
External References
Database Identifier Description
NBRP SPBC21.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC21.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC21.05c BioGRID Interaction Datasets
Expression Viewer SPBC21.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC21.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC21.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC21.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC21.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC21.05c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC21.05c Cell Cycle Data
GEO SPBC21.05c GEO profiles
PInt SPBC21.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC21.05c Peptides identified in tandem mass spectrometry proteomics experiments
EntrezGene2540760Ras1-Scd pathway protein Ral2
WikiGene2540760Ras1-Scd pathway protein Ral2
SPD / RIKEN33/33E05Orfeome Localization Data
UniProtKB/SwissProtP15258Protein ral2
ModBaseP15258Database of comparative protein structure models
STRINGP15258Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596339Ras1-Scd pathway protein Ral2
RefSeq mRNANM_001022260972h- Ras1-Scd pathway protein Ral2 (ral2), mRNA
European Nucleotide ArchiveAAA35331ENA Protein Mapping
European Nucleotide ArchiveAAA35331.1ENA Protein Mapping
European Nucleotide ArchiveCAB36885ENA Protein Mapping
European Nucleotide ArchiveCAB36885.1ENA Protein Mapping
UniParcUPI00001330F4UniProt Archive

Literature for ral2

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014