mcm6 (SPBC211.04c)


Gene Standard Namemcm6 Characterisation Statuspublished
Systematic IDSPBC211.04c Feature Typeprotein coding
Synonymsmis5 Name Description
ProductMCM complex subunit Mcm6 Product Size892aa, 99.55 kDa
Genomic Location Chromosome II, 3883065-3880098 (2968nt); CDS:3882996-3880318 (2679nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATP-dependent 3'-5' DNA helicase activity11
Annotation ExtensionEvidenceWith/FromReference
ATPase activity235
Annotation ExtensionEvidenceWith/FromReference
single-stranded DNA binding28
Annotation ExtensionEvidenceWith/FromReference
activated by ATP
  • activated by ATP
single-stranded DNA-dependent ATP-dependent DNA helicase activity6
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
double-strand break repair via homologous recombination32
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication70
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication initiation33
Annotation ExtensionEvidenceWith/FromReference
premeiotic DNA replication12
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationmcm6-HA (mcm6+, C-terminal 3 x HA)Not specified1427
mcm6-KS-AA (KS482AA)Not specified
mcm6-KS-AA-HA (KS482AA, N-terminal 3 x HA)Not specified
mcm6ΔNull
mcm6truncated-KS-AA-HA (KS482AA, del aa 864-892, N-terminal 3 x HA)Not specified
not recorded (wild type)
sensitive to camptothecinmcm6-S1 (E824G, D825N)214
sensitive to hydroxyureamis5-268518
sensitive to methyl methanesulfonatemcm6-S1 (E824G, D825N)227
sensitive to UV during vegetative growthmis5-268133
viable vegetative cell populationmcm6-DE-AA (DE540AA)Not specified3781
mcm6-DE-AA-HA (DE540AA, N-terminal 3 x HA)Not specified
mcm6-KA (K482A)Not specified
mcm6-KA-HA (K482A, N-terminal 3 x HA)Not specified
mcm6truncated-DA-HA (D540A, del aa 864-892, N-terminal 3 x HA)Not specified
mcm6truncated-DE-AA-HA (DE540AA, del aa 864-892, N-terminal 3 x HA)Not specified
mcm6truncated-KA-HA (K482A, del aa 864-892, N-terminal 3 x HA)Not specified
mcm6truncated-KR-HA (K482R, del aa 864-892, N-terminal 3 x HA)Not specified

Cell Phenotype

Term NameAlleleExpressionCount
abnormal DNA replicationmis5-26841
abnormal mitotic cell cyclemcm6ΔNull794
abolished protein binding68
affecting mcm6 and cdt1mcm6-S1 (E824G, D825N)
abolished protein localization to nucleus during vegetative growth33
affecting mcm4mis5-268Not specified
cell cycle arrest in mitotic S phasemis5-26810
decreased pre-replicative complex assemblymcm6-S1 (E824G, D825N)1
decreased protein level in germinating spore4
affecting mcm6mcm6Δ::his3+ Null
delayed double-strand break repairmcm6-S1 (E824G, D825N)Endogenous1
elongated aseptate cellmcm6Δ::his3+ Null52
increased duration of mitotic S phasemcm6Δ::his3+ Null13
increased plasmid lossmis5-268Not specified11
inviable after spore germination with elongated germ tubemcm6ΔNull163
inviable after spore germination, single or double cell divisionmcm6ΔNull101
inviable after spore germination, without cell division, cell cycle arrest with replicated DNAmcm6Δ::his3+ Null5
inviable cellmcm6ΔNull1458
inviable elongated mononucleate aseptate cellmis5-26837
normal protein phosphorylation34
affecting chk1mcm6-S1 (E824G, D825N)
protein mislocalized to cytoplasm34
affecting mcm7mis5-268Not specified
affecting mcm2mis5-268Not specified
affecting mcm4mis5-268Not specified
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cdc10 MBF transcription factor complex subunit Cdc10 PMID:10490657
FYPO affected by mutation in mcm2 MCM complex subunit Mcm2 PMID:9658174
FYPO affected by mutation in mcm2 MCM complex subunit Mcm2 PMID:10588642
FYPO affected by mutation in mcm3 MCM complex subunit Mcm3 PMID:10588642
FYPO affected by mutation in mcm4 MCM complex subunit Mcm4/Cdc21 PMID:10588642
FYPO affected by mutation in mcm6 MCM complex subunit Mcm6 PMID:21971174
FYPO affected by mutation in mcm6 MCM complex subunit Mcm6 PMID:9914167
FYPO affected by mutation in rpn12 19S proteasome regulatory subunit Rpn12 PMID:23349636
FYPO affected by mutation in swi1 replication fork protection complex subunit Swi1 PMID:23349636
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
138830653880098

UTRs

Region Coordinates Reference
five_prime_UTR3883065..3882997PMID:21511999
three_prime_UTR3880317..3880098PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00493 Pfam IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 414 736 6
PF14551 Pfam IPR027925 MCM N-terminal domain 73 214 6
SM00350 SMART IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 194 737 6
PS00847 Prosite Patterns IPR018525 Mini-chromosome maintenance, conserved site 535 543 6
PS50051 Prosite Profiles IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 426 632 6
PTHR11630 HMMPANTHER 26 882 6
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 26 882 1
1ltlA01 Gene3D 70 160 6
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 436 616 288
2.20.28.10 Gene3D IPR004039 Rubredoxin-type fold 226 275 3
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 197 224 48
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 277 327 48
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 436 749 294
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 76 328 57
Coil ncoils Rabaptin coiled-coil domain 739 765 971
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 140 152 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 298 309 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 245 262 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 355 376 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 195 207 1
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 606 614 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 467 482 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 527 541 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 579 591 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 555 568 6

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.60 Da
Charge -27.00
Isoelectric point 4.77
Molecular weight 99.55 kDa
Number of residues 892
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during cellular response to thiabendazoleS98
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S98 PMID:18257517
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0006279Western blot evidencePMID:10888871
present during GO:0051321Western blot evidencePMID:10888871
decreased during GO:0045023Western blot evidencePMID:16899242

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2258during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3865during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2493during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2059.12during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1292.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
helicase activity of Mcm4/6/7 complex requires 5' overhang PMID:111362473
processive helicase activity of Mcm4/6/7 complex requires 3' and 5' overhangs PMID:111362473
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
cdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:12006645
Affinity Capture-WesternPMID:15338237
Affinity Capture-WesternPMID:11344166
cdt1replication licensing factor Cdt1 Two-hybridPMID:21971174
Affinity Capture-Western
mcm6MCM complex subunit Mcm6 Co-purificationPMID:23444842
cdc23MCM-associated protein Mcm10 Two-hybridPMID:12185500
Two-hybridPMID:22433840
Two-hybridPMID:23695164
bis1stress response protein Bis1 Two-hybridPMID:11751918
mcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:9658174
Affinity Capture-WesternPMID:10770926
Affinity Capture-WesternPMID:21813639
Affinity Capture-WesternPMID:9366552
Co-purification
mcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:11973289
Affinity Capture-WesternPMID:21813639
Affinity Capture-WesternPMID:10770926
Affinity Capture-WesternPMID:9658174
Co-purificationPMID:23444842
Reconstituted ComplexPMID:11136247
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
psf2GINS complex subunit Psf2 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:22433840
mcm5MCM complex subunit Mcm5 Affinity Capture-WesternPMID:9658174
mcb1MCM binding protein homolog Mcb1 Affinity Capture-WesternPMID:22036784
Affinity Capture-MS
Reconstituted ComplexPMID:23322785
Affinity Capture-Western
Two-hybrid
Affinity Capture-WesternPMID:21813639
sld3DNA replication pre-initiation complex subunit Sld3 Affinity Capture-WesternPMID:12006645
mcm7MCM complex subunit Mcm7 Co-purificationPMID:9705352
Affinity Capture-WesternPMID:21813639
Co-purificationPMID:10770926
Co-purificationPMID:23444842
Affinity Capture-WesternPMID:11606526
Reconstituted ComplexPMID:11136247
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
hhf3histone H4 h4.3 Dosage RescuePMID:15507118
bir1survivin, Bir1 Dosage RescuePMID:15507118
cut1separase/separin Dosage RescuePMID:15507118
cig2G1/S-specific B-type cyclin Cig2 Synthetic RescuePMID:21971174
Phenotypic Suppression
Synthetic RescuePMID:15194812
cdt1replication licensing factor Cdt1 Synthetic RescuePMID:21971174
Phenotypic Suppression
dis1microtubule-associated protein Dis1 Dosage RescuePMID:15507118
rum1CDK inhibitor Rum1 Dosage RescuePMID:15194812
cdc23MCM-associated protein Mcm10 Synthetic Growth DefectPMID:12185500
cdc18MCM loader Synthetic RescuePMID:21971174
mcm2MCM complex subunit Mcm2 Dosage Growth DefectPMID:9383050
Dosage RescuePMID:15507118
mcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:11606526
Synthetic LethalityPMID:7865880
alp14TOG ortholog Alp14 Dosage RescuePMID:15507118
hht3histone H3 h3.3 Dosage RescuePMID:15507118
mcm5MCM complex subunit Mcm5 Dosage RescuePMID:15507118
mcb1MCM binding protein homolog Mcb1 Synthetic Growth DefectPMID:23322785
Dosage Rescue
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic Growth DefectPMID:11027263
mcm7MCM complex subunit Mcm7 Synthetic LethalityPMID:11606526
External References
Database Identifier Description
NBRP SPBC211.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC211.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC211.04c BioGRID Interaction Datasets
Expression Viewer SPBC211.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC211.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC211.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC211.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC211.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC211.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC211.04c Cell Cycle Data
GEO SPBC211.04c GEO profiles
PInt SPBC211.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC211.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC211.04c Fission yeast phenotypic data & analysis
SPD / RIKEN40/40H11Orfeome Localization Data
UniProtKB/SwissProtP49731DNA replication licensing factor mcm6
ModBaseP49731Database of comparative protein structure models
STRINGP49731Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596614MCM complex subunit Mcm6
RefSeq mRNANM_001022535972h- MCM complex subunit Mcm6 (mcm6), mRNA
European Nucleotide ArchiveBAA06729.1ENA Protein Mapping
European Nucleotide ArchiveCAB75412.1ENA Protein Mapping
UniParcUPI000012F162UniProt Archive

Literature for mcm6

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015