mcm6 (SPBC211.04c)


Gene Standard Namemcm6 Characterisation Statuspublished
Systematic IDSPBC211.04c Feature Typeprotein coding
Synonymsmis5 Name Description
ProductMCM complex subunit Mcm6 Product Size892aa, 99.55 kDa
Genomic Location Chromosome II, 3883065-3880098 (2968nt); CDS:3882996-3880318 (2679nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
3'-5' DNA/RNA helicase activity3
Annotation ExtensionEvidenceWith/FromReference
ATP binding557
Annotation ExtensionEvidenceWith/FromReference
ATPase activity238
Annotation ExtensionEvidenceWith/FromReference
protein binding887
Annotation ExtensionEvidenceWith/FromReference
single-stranded DNA binding37
Annotation ExtensionEvidenceWith/FromReference
activated by ATP
  • activated by ATP
single-stranded DNA-dependent ATP-dependent 3'-5' DNA helicase activity5
Annotation ExtensionEvidenceWith/FromReference
single-stranded RNA binding16
Annotation ExtensionEvidenceWith/FromReference
NOT RNA helicase activity
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
double-strand break repair via homologous recombination38
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication77
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication initiation32
Annotation ExtensionEvidenceWith/FromReference
premeiotic DNA replication15
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
DNA replication preinitiation complex18
Annotation ExtensionEvidenceWith/FromReference
MCM complex6
Annotation ExtensionEvidenceWith/FromReference
MCM core complex3
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin365
nuclear pre-replicative complex14
Annotation ExtensionEvidenceWith/FromReference
nuclear replication fork62
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationmcm6-KS-AA (KS482AA)1455
mcm6+ (wild type)
mcm6truncated-KS-AA-HA (KS482AA, del aa 864-892, N-terminal 3 x HA)
mcm6-HA (mcm6+, C-terminal 3 x HA)
mcm6Δ
mcm6-KS-AA-HA (KS482AA, N-terminal 3 x HA)
sensitive to camptothecinmcm6-S1 (E824G, D825N)257
sensitive to hydroxyureamis5-268594
sensitive to methyl methanesulfonatemcm6-S1 (E824G, D825N)331
sensitive to UV during vegetative growthmis5-268161
viable vegetative cell populationmcm6truncated-KR-HA (K482R, del aa 864-892, N-terminal 3 x HA)3850
mcm6truncated-KA-HA (K482A, del aa 864-892, N-terminal 3 x HA)
mcm6-KA (K482A)
mcm6-DE-AA-HA (DE540AA, N-terminal 3 x HA)
mcm6truncated-DA-HA (D540A, del aa 864-892, N-terminal 3 x HA)
mcm6truncated-DE-AA-HA (DE540AA, del aa 864-892, N-terminal 3 x HA)
mcm6-DE-AA (DE540AA)
mcm6-KA-HA (K482A, N-terminal 3 x HA)

Cell Phenotype

Term NameGenotypesCount
abnormal cell cycle arrest in mitotic S phasemis5-26817
abnormal mitotic cell cyclemcm6Δ894
abnormal mitotic DNA replicationmis5-26828
abolished protein binding147
affecting mcm6 and cdt1mcm6-S1 (E824G, D825N)
abolished protein localization to nucleus during vegetative growth66
affects localization of mcm4mis5-268
decreased pre-replicative complex assemblymcm6-S1 (E824G, D825N)1
decreased protein level in germinating spore4
affecting mcm6mcm6Δ::his3+
delayed onset of double-strand break repairmcm6-S1 (E824G, D825N)2
elongated aseptate vegetative cellmcm6Δ::his3+ 72
increased duration of mitotic S phasemcm6Δ::his3+ 23
increased number of double-strand break sites during vegetative growthmis5-26819
increased number of Rad52 foci during vegetative growthmis5-26845
increased plasmid lossmis5-26813
inviable after spore germination with elongated germ tubemcm6Δ164
inviable after spore germination, single or double cell divisionmcm6Δ107
inviable after spore germination, without cell division, cell cycle arrest with replicated DNAmcm6Δ::his3+ 6
inviable elongated mononucleate aseptate cellmis5-26844
inviable elongated mononucleate aseptate vegetative cell with cell cycle arrest in mitotic G2 phasemis5-2267
mis5-628
normal protein phosphorylation during vegetative growth96
affecting chk1mcm6-S1 (E824G, D825N)
protein mislocalized to cytoplasm during vegetative growth66
affects localization of mcm7mis5-268
affects localization of mcm2mis5-268
affects localization of mcm4mis5-268

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationmis5-268, mcm6-HA (mcm6+, C-terminal 3 x HA)
mis5-268, cdc23-M36 (D232G, V265I)
cdc23-1E2 (C239Y), mis5-268
normal growth on camptothecincig2Δ, mcm6-S1 (E824G, D825N)
cdt1+, mcm6-S1 (E824G, D825N)
cdc18+ (wild type), mcm6-S1 (E824G, D825N)
normal growth on methyl methanesulfonatecdt1+, mcm6-S1 (E824G, D825N)
sensitive to methyl methanesulfonatecdc18+ (wild type), mcm6-S1 (E824G, D825N)
cig2Δ, mcm6-S1 (E824G, D825N)
viable vegetative cell populationmis5-268, mcm6-HA (mcm6+, C-terminal 3 x HA)

Cell Phenotype

Term NameGenotypes
inviable after spore germination, without cell divisioncdc21-M68, mis5-268
normal double-strand break repaircig2Δ, mcm6-S1 (E824G, D825N)
cdc18+ (wild type), mcm6-S1 (E824G, D825N)
cdt1+, mcm6-S1 (E824G, D825N)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in cdc45 DNA replication pre-initiation complex subunit Cdc45
FYPO affected by mutation in mcm2 MCM complex subunit Mcm2
FYPO affected by mutation in mcm6 MCM complex subunit Mcm6
FYPO affected by mutation in rpn12 19S proteasome regulatory subunit Rpn12
FYPO affected by mutation in swi1 replication fork protection complex subunit Swi1
FYPO localization affected by mutation in cdc10 MBF transcription factor complex subunit Cdc10
FYPO localization affected by mutation in cdc18 MCM loader
FYPO localization affected by mutation in mcm2 MCM complex subunit Mcm2
FYPO localization affected by mutation in mcm3 MCM complex subunit Mcm3
FYPO localization affected by mutation in mcm4 MCM complex subunit Mcm4/Cdc21
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3883065..3880098
mRNA3883065..3880098
5' UTR3883065..3882997PMID:21511999
CDS3882996..3880318
3' UTR3880317..3880098PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00493 Pfam IPR001208 MCM domain 414 736 6
PF17207 Pfam IPR033762 MCM OB domain 198 326 6
PF14551 Pfam IPR027925 MCM N-terminal domain 73 191 6
SM00350 SMART IPR031327 Mini-chromosome maintenance protein 194 737 6
PS00847 Prosite Patterns IPR018525 Mini-chromosome maintenance, conserved site 535 543 6
PS50051 Prosite Profiles IPR001208 MCM domain 426 632 6
PTHR11630 HMMPANTHER 364 383 6
PTHR11630 HMMPANTHER 401 762 6
PTHR11630 HMMPANTHER 784 856 6
PTHR11630 HMMPANTHER 35 340 6
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 364 383 1
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 401 762 1
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 784 856 1
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 35 340 1
1ltlA01 Gene3D 70 160 6
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 436 616 288
2.40.50.140 Gene3D RecQ-mediated genome instability protein 2 277 327 48
2.40.50.140 Gene3D RecQ-mediated genome instability protein 2 197 224 48
2.20.28.10 Gene3D IPR004039 Rubredoxin-type fold 226 275 3
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 76 328 57
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 436 749 294
Coil ncoils Predicted coiled-coil protein (DUF2205) 739 764 1055
PR01657 PRINTS IPR001208 MCM domain 467 482 6
PR01657 PRINTS IPR001208 MCM domain 527 541 6
PR01657 PRINTS IPR001208 MCM domain 555 568 6
PR01657 PRINTS IPR001208 MCM domain 606 614 6
PR01657 PRINTS IPR001208 MCM domain 579 591 6
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 195 207 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 245 262 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 355 376 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 298 309 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 140 152 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.60 Da
Charge -27.00
Codon Adaptation Index 0.40
Isoelectric point 4.77
Molecular weight 99.55 kDa
Number of residues 892
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during cellular response to thiabendazoleS98
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S98 PMID:18257517
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelconstant during GO:0000278Western blot evidencePMID:11344166
present during GO:0051321Western blot evidencePMID:10888871
constant during GO:0000278Western blot evidencePMID:9658174
increased during GO:0006279Western blot evidencePMID:10888871
decreased during GO:0045023Western blot evidencePMID:16899242

Quantitative Gene Expression

View graphical display of gene expression data for mcm6 (SPBC211.04c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2258during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3865during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2059.12during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2493during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1292.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
helicase activity of Mcm4/6/7 complex requires 5' overhang PMID:111362473
processive helicase activity of Mcm4/6/7 complex requires 3' and 5' overhangs PMID:111362473
MCM4/6/7 helicase complex binds a 3' overhang and translocates along single-stranded DNA in the 3' to 5' direction PMID:129753643
MCM4/6/7 helicase complex translocates along double-stranded DNA PMID:129753643
MCM4/6/7 helicase complex translocates along DNA/RNA duplex PMID:168295183
MCM4/6/7 helicase complex binds a 3' single-stranded DNA overhang and translocates along an DNA/RNA duplex in the 3' to 5' direction PMID:168295183
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in archaea242
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC211.04c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bymcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:9366552
affinity captured bymcb1MCM binding protein homolog Mcb1 Affinity Capture-WesternPMID:23322785
affinity captured bypsf2GINS complex subunit Psf2 Affinity Capture-WesternPMID:22718908
affinity captured bymcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:11973289
affinity captured bysld3DNA replication pre-initiation complex subunit Sld3 Affinity Capture-WesternPMID:12006645
affinity captured bycdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:15338237
affinity captured bymcm7MCM complex subunit Mcm7 Affinity Capture-WesternPMID:21813639
co-purifies withmcm2MCM complex subunit Mcm2 Co-purificationPMID:9366552
co-purifies withmcm7MCM complex subunit Mcm7 Co-purificationPMID:9705352
co-purifies withmcm6MCM complex subunit Mcm6 Co-purificationPMID:23444842
co-purifies withmcm4MCM complex subunit Mcm4/Cdc21 Co-purificationPMID:23444842
binds DNA-binding domain construct withcdc23MCM-associated protein Mcm10 Two-hybridPMID:26771498
binds DNA-binding domain construct withcdt1replication licensing factor Cdt1 Two-hybridPMID:21971174
binds DNA-binding domain construct withbis1splicing factor Bis1 Two-hybridPMID:11751918
binds DNA-binding domain construct withcaf4CCR4-Not complex subunit Caf4/Mdv1 (predicted) Two-hybridPMID:26771498
binds DNA-binding domain construct withmcb1MCM binding protein homolog Mcb1 Two-hybridPMID:23322785
interacts withcdc23MCM-associated protein Mcm10 PCAPMID:26771498
binds activation domain construct withmcm3MCM complex subunit Mcm3 Two-hybridPMID:26771498
binds activation domain construct withphp2CCAAT-binding factor complex subunit Php2 Two-hybridPMID:26771498
forms complex withmcm7MCM complex subunit Mcm7 Reconstituted ComplexPMID:11136247
forms complex withmcm4MCM complex subunit Mcm4/Cdc21 Reconstituted ComplexPMID:11136247
forms complex withmcb1MCM binding protein homolog Mcb1 Reconstituted ComplexPMID:23322785
affinity capturescdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:15338237
affinity capturesmcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:9658174
affinity capturesmcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:9658174
affinity capturescdt1replication licensing factor Cdt1 Affinity Capture-WesternPMID:21971174
affinity capturesmcm5MCM complex subunit Mcm5 Affinity Capture-WesternPMID:9658174
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC211.04c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withmcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:7865880
synthetic lethal withmcm7MCM complex subunit Mcm7 Synthetic LethalityPMID:11606526
overexpression causes growth defect tomcm2MCM complex subunit Mcm2 Dosage Growth DefectPMID:9383050
rescued by overexpression ofalp14TOG/XMAP14 family protein Alp14 Dosage RescuePMID:15507118
rescued by overexpression ofrum1CDK inhibitor Rum1 Dosage RescuePMID:15194812
rescued by overexpression ofhht3histone H3 h3.3 Dosage RescuePMID:15507118
rescued by overexpression ofmcm5MCM complex subunit Mcm5 Dosage RescuePMID:15507118
rescued by overexpression ofbir1survivin, Bir1 Dosage RescuePMID:15507118
rescued by overexpression ofcut1separase/separin Dosage RescuePMID:15507118
rescued by overexpression ofhhf3histone H4 h4.3 Dosage RescuePMID:15507118
rescued by overexpression ofmcm2MCM complex subunit Mcm2 Dosage RescuePMID:15507118
rescued by overexpression ofdis1TOG/XMAP14 microtubule-associated protein Dis1 Dosage RescuePMID:15507118
rescued bycig2G1/S-specific B-type cyclin Cig2 Phenotypic SuppressionPMID:21971174
rescued bycdt1replication licensing factor Cdt1 Phenotypic SuppressionPMID:21971174
synthetically rescued bycdt1replication licensing factor Cdt1 Synthetic RescuePMID:21971174
synthetically rescued bycig2G1/S-specific B-type cyclin Cig2 Synthetic RescuePMID:21971174
synthetically rescued bycdc18MCM loader Synthetic RescuePMID:21971174
synthetic growth defect withmcb1MCM binding protein homolog Mcb1 Synthetic Growth DefectPMID:23322785
synthetic growth defect withhsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic Growth DefectPMID:11027263
synthetic growth defect withcdc23MCM-associated protein Mcm10 Synthetic Growth DefectPMID:12185500
overexpression rescuesmcb1MCM binding protein homolog Mcb1 Dosage RescuePMID:23322785
External References
Database Identifier Description
NBRP SPBC211.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC211.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC211.04c BioGRID Interaction Datasets
Expression Viewer SPBC211.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC211.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC211.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC211.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC211.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC211.04c Transcriptome Viewer (Bähler Lab)
GEO SPBC211.04c GEO profiles
PInt SPBC211.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC211.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC211.04c Fission yeast phenotypic data & analysis
Cyclebase SPBC211.04c.1 Cell Cycle Data
SPD / RIKEN40/40H11Orfeome Localization Data
UniProtKB/SwissProtP49731DNA replication licensing factor mcm6
ModBaseP49731Database of comparative protein structure models
STRINGP49731Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596614MCM complex subunit Mcm6
RefSeq mRNANM_001022535972h- MCM complex subunit Mcm6 (mcm6), mRNA
European Nucleotide ArchiveBAA06729.1ENA Protein Mapping
European Nucleotide ArchiveCAB75412.1ENA Protein Mapping
UniParcUPI000012F162UniProt Archive

Literature for mcm6

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016