mcm6 (SPBC211.04c)


Gene Standard Namemcm6 Characterisation Statuspublished
Systematic IDSPBC211.04c Feature Typeprotein coding
Synonyms Name Description
ProductMCM complex subunit Mcm6 Product Size892aa, 99.55 kDa
Genomic Location Chromosome II, 3883065-3880098 (2968nt); CDS:3882996-3880318 (2679nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
3'-5' DNA/RNA helicase activity3
Annotation ExtensionEvidenceWith/FromReference
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
ATPase activity239
Annotation ExtensionEvidenceWith/FromReference
protein binding862
Annotation ExtensionEvidenceWith/FromReference
single-stranded DNA binding27
Annotation ExtensionEvidenceWith/FromReference
activated by ATP
  • activated by ATP
single-stranded DNA-dependent ATP-dependent 3'-5' DNA helicase activity4
Annotation ExtensionEvidenceWith/FromReference
single-stranded RNA binding15
Annotation ExtensionEvidenceWith/FromReference
NOT RNA helicase activity
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
double-strand break repair via homologous recombination36
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication76
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication initiation32
Annotation ExtensionEvidenceWith/FromReference
premeiotic DNA replication12
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
DNA replication preinitiation complex18
Annotation ExtensionEvidenceWith/FromReference
MCM complex6
Annotation ExtensionEvidenceWith/FromReference
MCM core complex3
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin344
nuclear pre-replicative complex14
Annotation ExtensionEvidenceWith/FromReference
nuclear replication fork61
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationmcm6truncated-KS-AA-HA (KS482AA, del aa 864-892, N-terminal 3 x HA)1450
mcm6-HA (mcm6+, C-terminal 3 x HA)
mcm6-KS-AA-HA (KS482AA, N-terminal 3 x HA)
mcm6-KS-AA (KS482AA)
mcm6Δ
mcm6+ (wild type)
sensitive to camptothecinmcm6-S1 (E824G, D825N)227
sensitive to hydroxyureamis5-268547
sensitive to methyl methanesulfonatemcm6-S1 (E824G, D825N)250
sensitive to UV during vegetative growthmis5-268148
viable vegetative cell populationmcm6-DE-AA (DE540AA)3831
mcm6-KA (K482A)
mcm6truncated-KA-HA (K482A, del aa 864-892, N-terminal 3 x HA)
mcm6-DE-AA-HA (DE540AA, N-terminal 3 x HA)
mcm6truncated-DE-AA-HA (DE540AA, del aa 864-892, N-terminal 3 x HA)
mcm6truncated-KR-HA (K482R, del aa 864-892, N-terminal 3 x HA)
mcm6-KA-HA (K482A, N-terminal 3 x HA)
mcm6truncated-DA-HA (D540A, del aa 864-892, N-terminal 3 x HA)

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclemcm6Δ849
abnormal mitotic DNA replicationmis5-26827
abolished protein binding121
affecting mcm6 and cdt1mcm6-S1 (E824G, D825N)
abolished protein localization to nucleus during vegetative growth54
affecting mcm4mis5-268
cell cycle arrest in mitotic S phasemis5-26815
decreased pre-replicative complex assemblymcm6-S1 (E824G, D825N)1
decreased protein level in germinating spore4
affecting mcm6mcm6Δ::his3+
delayed onset of double-strand break repairmcm6-S1 (E824G, D825N)2
elongated aseptate vegetative cellmcm6Δ::his3+ 63
increased duration of mitotic S phasemcm6Δ::his3+ 22
increased number of double-strand break sites during vegetative growthmis5-26819
increased number of Rad52 foci during vegetative growthmis5-26831
increased plasmid lossmis5-26813
inviable after spore germination with elongated germ tubemcm6Δ164
inviable after spore germination, single or double cell divisionmcm6Δ106
inviable after spore germination, without cell division, cell cycle arrest with replicated DNAmcm6Δ::his3+ 6
inviable elongated mononucleate aseptate cellmis5-26840
normal protein phosphorylation during vegetative growth64
affecting chk1mcm6-S1 (E824G, D825N)
protein mislocalized to cytoplasm during vegetative growth49
affecting mcm7mis5-268
affecting mcm2mis5-268
affecting mcm4mis5-268

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationcdc23-1E2 (C239Y), mis5-268
mis5-268, cdc23-M36 (D232G, V265I)
mcm6-HA (mcm6+, C-terminal 3 x HA), mis5-268
normal growth on camptothecincig2Δ, mcm6-S1 (E824G, D825N)
mcm6-S1 (E824G, D825N), cdc18+ (wild type)
cdt1+, mcm6-S1 (E824G, D825N)
normal growth on methyl methanesulfonatecdt1+, mcm6-S1 (E824G, D825N)
sensitive to methyl methanesulfonatemcm6-S1 (E824G, D825N), cdc18+ (wild type)
cig2Δ, mcm6-S1 (E824G, D825N)
viable vegetative cell populationmcm6-HA (mcm6+, C-terminal 3 x HA), mis5-268

Cell Phenotype

Term NameGenotypes
inviable after spore germination, without cell divisioncdc21-M68, mis5-268
normal double-strand break repaircdt1+, mcm6-S1 (E824G, D825N)
cig2Δ, mcm6-S1 (E824G, D825N)
mcm6-S1 (E824G, D825N), cdc18+ (wild type)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in cdc10 MBF transcription factor complex subunit Cdc10
FYPO affected by mutation in cdc18 MCM loader
FYPO affected by mutation in cdc45 DNA replication pre-initiation complex subunit Cdc45
FYPO affected by mutation in mcm2 MCM complex subunit Mcm2
FYPO affected by mutation in mcm3 MCM complex subunit Mcm3
FYPO affected by mutation in mcm4 MCM complex subunit Mcm4/Cdc21
FYPO affected by mutation in mcm6 MCM complex subunit Mcm6
FYPO affected by mutation in rpn12 19S proteasome regulatory subunit Rpn12
FYPO affected by mutation in swi1 replication fork protection complex subunit Swi1
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3883065..3880098
5' UTR3883065..3882997PMID:21511999
3' UTR3880317..3880098PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00493 Pfam IPR001208 MCM domain 414 736 6
PF14551 Pfam IPR027925 MCM N-terminal domain 73 214 6
SM00350 SMART IPR031327 Mini-chromosome maintenance protein 194 737 6
PS00847 Prosite Patterns IPR018525 Mini-chromosome maintenance, conserved site 535 543 6
PS50051 Prosite Profiles IPR001208 MCM domain 426 632 6
PTHR11630 HMMPANTHER 35 340 6
PTHR11630 HMMPANTHER 364 383 6
PTHR11630 HMMPANTHER 401 762 6
PTHR11630 HMMPANTHER 784 856 6
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 35 340 1
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 364 383 1
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 401 762 1
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 784 856 1
1ltlA01 Gene3D 70 160 6
2.20.28.10 Gene3D IPR004039 Rubredoxin-type fold 226 275 3
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 197 224 48
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 277 327 48
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 436 616 288
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 76 328 57
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 436 749 294
Coil ncoils Predicted coiled-coil protein (DUF2205) 739 764 1050
PR01657 PRINTS IPR001208 MCM domain 467 482 6
PR01657 PRINTS IPR001208 MCM domain 527 541 6
PR01657 PRINTS IPR001208 MCM domain 555 568 6
PR01657 PRINTS IPR001208 MCM domain 579 591 6
PR01657 PRINTS IPR001208 MCM domain 606 614 6
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 140 152 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 195 207 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 245 262 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 298 309 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 355 376 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.60 Da
Charge -27.00
Codon Adaptation Index 0.40
Isoelectric point 4.77
Molecular weight 99.55 kDa
Number of residues 892
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1675
present during cellular response to thiabendazoleS98
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S98 PMID:18257517
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelconstant during GO:0000278Western blot evidencePMID:11344166
increased during GO:0006279Western blot evidencePMID:10888871
present during GO:0051321Western blot evidencePMID:10888871
decreased during GO:0045023Western blot evidencePMID:16899242
constant during GO:0000278Western blot evidencePMID:9658174

Quantitative Gene Expression

View graphical display of gene expression data for mcm6 (SPBC211.04c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2258during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3865during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2493during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2059.12during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1292.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
helicase activity of Mcm4/6/7 complex requires 5' overhang PMID:111362473
processive helicase activity of Mcm4/6/7 complex requires 3' and 5' overhangs PMID:111362473
MCM4/6/7 helicase complex binds a 3' overhang and translocates along single-stranded DNA in the 3' to 5' direction PMID:129753643
MCM4/6/7 helicase complex translocates along double-stranded DNA PMID:129753643
MCM4/6/7 helicase complex translocates along DNA/RNA duplex PMID:168295183
MCM4/6/7 helicase complex binds a 3' single-stranded DNA overhang and translocates along an DNA/RNA duplex in the 3' to 5' direction PMID:168295183
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
conserved in archaea238
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPBC211.04c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
co-purifies withmcm2MCM complex subunit Mcm2 Co-purificationPMID:9366552
co-purifies withmcm6MCM complex subunit Mcm6 Co-purificationPMID:23444842
co-purifies withmcm7MCM complex subunit Mcm7 Co-purificationPMID:23444842
co-purifies withmcm4MCM complex subunit Mcm4/Cdc21 Co-purificationPMID:23444842
affinity captured bymcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:9366552
affinity captured bymcb1MCM binding protein homolog Mcb1 Affinity Capture-WesternPMID:22036784
affinity captured bypsf2GINS complex subunit Psf2 Affinity Capture-WesternPMID:22718908
affinity captured bycdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:15338237
affinity captured bymcm7MCM complex subunit Mcm7 Affinity Capture-WesternPMID:21813639
affinity captured bymcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:9658174
affinity captured bysld3DNA replication pre-initiation complex subunit Sld3 Affinity Capture-WesternPMID:12006645
affinity capturescdt1replication licensing factor Cdt1 Affinity Capture-WesternPMID:21971174
affinity capturescdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:12006645
affinity capturesmcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:9658174
affinity capturesmcm5MCM complex subunit Mcm5 Affinity Capture-WesternPMID:9658174
affinity capturesmcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:9658174
forms complex withmcb1MCM binding protein homolog Mcb1 Reconstituted ComplexPMID:23322785
forms complex withmcm4MCM complex subunit Mcm4/Cdc21 Reconstituted ComplexPMID:11136247
forms complex withmcm7MCM complex subunit Mcm7 Reconstituted ComplexPMID:11136247
binds DNA-binding domain construct withcdc23MCM-associated protein Mcm10 Two-hybridPMID:12185500
binds DNA-binding domain construct withcdt1replication licensing factor Cdt1 Two-hybridPMID:21971174
binds DNA-binding domain construct withmcb1MCM binding protein homolog Mcb1 Two-hybridPMID:23322785
binds DNA-binding domain construct withbis1splicing factor Bis1 Two-hybridPMID:11751918
Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPBC211.04c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
overexpression causes growth defect tomcm2MCM complex subunit Mcm2 Dosage Growth DefectPMID:9383050
synthetic lethal withmcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:7865880
synthetic lethal withmcm7MCM complex subunit Mcm7 Synthetic LethalityPMID:11606526
rescued by overexpression ofmcm5MCM complex subunit Mcm5 Dosage RescuePMID:15507118
rescued by overexpression ofalp14TOG/XMAP14 family protein Alp14 Dosage RescuePMID:15507118
rescued by overexpression ofcut1separase/separin Dosage RescuePMID:15507118
rescued by overexpression ofhht3histone H3 h3.3 Dosage RescuePMID:15507118
rescued by overexpression ofrum1CDK inhibitor Rum1 Dosage RescuePMID:15194812
rescued by overexpression ofdis1TOG/XMAP14 microtubule-associated protein Dis1 Dosage RescuePMID:15507118
rescued by overexpression ofmcm2MCM complex subunit Mcm2 Dosage RescuePMID:15507118
rescued by overexpression ofhhf3histone H4 h4.3 Dosage RescuePMID:15507118
rescued by overexpression ofbir1survivin, Bir1 Dosage RescuePMID:15507118
synthetically rescued bycig2G1/S-specific B-type cyclin Cig2 Synthetic RescuePMID:21971174
synthetically rescued bycdt1replication licensing factor Cdt1 Synthetic RescuePMID:21971174
synthetically rescued bycdc18MCM loader Synthetic RescuePMID:21971174
rescued bycig2G1/S-specific B-type cyclin Cig2 Phenotypic SuppressionPMID:21971174
rescued bycdt1replication licensing factor Cdt1 Phenotypic SuppressionPMID:21971174
synthetic growth defect withcdc23MCM-associated protein Mcm10 Synthetic Growth DefectPMID:12185500
synthetic growth defect withmcb1MCM binding protein homolog Mcb1 Synthetic Growth DefectPMID:23322785
synthetic growth defect withhsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic Growth DefectPMID:11027263
overexpression rescuesmcb1MCM binding protein homolog Mcb1 Dosage RescuePMID:23322785
External References
Database Identifier Description
NBRP SPBC211.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC211.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC211.04c BioGRID Interaction Datasets
Expression Viewer SPBC211.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC211.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC211.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC211.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC211.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC211.04c Transcriptome Viewer (Bähler Lab)
GEO SPBC211.04c GEO profiles
PInt SPBC211.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC211.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC211.04c Fission yeast phenotypic data & analysis
Cyclebase SPBC211.04c.1 Cell Cycle Data
SPD / RIKEN40/40H11Orfeome Localization Data
UniProtKB/SwissProtP49731DNA replication licensing factor mcm6
ModBaseP49731Database of comparative protein structure models
STRINGP49731Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596614MCM complex subunit Mcm6
RefSeq mRNANM_001022535972h- MCM complex subunit Mcm6 (mcm6), mRNA
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveD31960ENA EMBL mapping
European Nucleotide ArchiveBAA06729ENA Protein Mapping
European Nucleotide ArchiveCAB75412ENA Protein Mapping
UniParcUPI000012F162UniProt Archive

Literature for mcm6

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Release Version: PomBase:30_57 - 27 Jan 2016