mcm6 (SPBC211.04c)


Gene Standard Namemcm6 Characterisation Statuspublished
Systematic IDSPBC211.04c Feature Typeprotein coding
Synonymsmis5 Name Description
ProductMCM complex subunit Mcm6 Product Size892aa, 99.55 kDa
Genomic Location Chromosome II, 3883065-3880098 (2968nt); CDS:3882996-3880318 (2679nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0043140ATP-dependent 3'-5' DNA helicase activity10
contributes_toIDAPMID:10770926
GO:0016887ATPase activity232
contributes_toIDAPMID:10770926
GO:0003697single-stranded DNA binding28
contributes_toIDAPMID:10770926
activated by ATP
GO:0017116single-stranded DNA-dependent ATP-dependent DNA helicase activity5
contributes_toIDAPMID:10770926
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1902975DNA replication initiation involved in mitotic DNA replicationIEAIPR008049GO_REF:000000229
GO:0000724double-strand break repair via homologous recombinationIMPPMID:2197117431
IPIcdt1PMID:21971174
GO:1902969mitotic cell cycle DNA replicationIMPPMID:786588016
GO:0006279premeiotic DNA replicationICGO:0000790PMID:118544028
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031261DNA replication preinitiation complexICGO:0042555GO_REF:000000121
GO:0042555MCM complexIDAPMID:93665526
GO:0097373MCM core complexIDAPMID:111362473
IDAPMID:9658174
IDAPMID:10770926
IDAPMID:23444842
GO:0005829cytosolIDAPMID:168233722319
GO:0000790nuclear chromatin278
during mitotic S phase, during mitotic G1 phaseIDAPMID:10490657
during meiotic S phaseIDAPMID:11854402
part of origin_of_replicationIDAPMID:10490657
during premeiotic DNA replicationIDAPMID:11854402
GO:0005656nuclear pre-replicative complexICGO:0042555GO_REF:000000114
GO:0043596nuclear replication forkICGO:0042555PMID:936655264
GO:0005634nucleusIDAPMID:105886422737
IDAPMID:10490657
IMPPMID:7865880
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopymcm6ΔNullPECO:0000005, PECO:0000137PMID:236978061337
Microscopymcm6ΔNullPMID:20473289
FYPO:0000268sensitive to UV during vegetative growthCell growth assaymis5-268PECO:0000005PMID:7865880120
FYPO:0000085sensitive to camptothecinCell growth assaymcm6-S1 (E824G, D825N)PECO:0000004PMID:21971174211
Cell growth assaymcm6-S1 (E824G, D825N)PECO:0000005PMID:21971174
FYPO:0000088sensitive to hydroxyureaCell growth assaymis5-268PECO:0000005PMID:7865880513
FYPO:0000089sensitive to methyl methanesulfonateCell growth assaymcm6-S1 (E824G, D825N)PECO:0000004PMID:21971174214
Cell growth assaymcm6-S1 (E824G, D825N)PECO:0000005PMID:21971174

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000217abnormal DNA replicationFlow cytometry datamis5-268PECO:0000004PMID:786588025
FYPO:0000059abnormal mitotic cell cycleMicroscopymcm6ΔNullPECO:0000005, PECO:0000137PMID:23697806640
FYPO:0000705abolished protein binding56
affecting mcm6 and cdt1Co-immunoprecipitation experimentmcm6-S1 (E824G, D825N)PMID:21971174
FYPO:0001424abolished protein localization to nucleus during vegetative growth24
affecting mcm4Microscopymis5-268Not specifiedPECO:0000004PMID:10588642
FYPO:0000611cell cycle arrest in mitotic S phaseFlow cytometry datamis5-268PECO:0000004PMID:78658805
FYPO:0000774decreased pre-replicative complex assemblyChromatin immunoprecipitation experimentmcm6-S1 (E824G, D825N)PMID:219711741
FYPO:0001388decreased protein level in germinating spore4
affecting mcm6Western blot assaymcm6Δ::his3+ NullPECO:0000005PMID:9914167
FYPO:0000778delayed double-strand break repairMicroscopymcm6-S1 (E824G, D825N)EndogenousPMID:219711741
FYPO:0000950elongated aseptate cellMicroscopymcm6Δ::his3+ NullPECO:0000005PMID:991416745
FYPO:0000614increased duration of mitotic S phaseFlow cytometry datamcm6Δ::his3+ NullPECO:0000005PMID:991416711
FYPO:0000786increased plasmid lossPlasmid maintenance assay evidencemis5-268Not specifiedPECO:0000005PMID:786588011
FYPO:0000314inviable after spore germination with elongated germ tubeMicroscopymcm6ΔNullPECO:0000005, PECO:0000137PMID:23697806163
FYPO:0001042inviable after spore germination, single or double cell divisionMicroscopymcm6ΔNullPECO:0000005, PECO:0000137PMID:2369780696
FYPO:0001385inviable after spore germination, without cell division, cell cycle arrest with replicated DNAFlow cytometry datamcm6Δ::his3+ NullPECO:0000005PMID:99141675
FYPO:0000049inviable cellCell growth assaymcm6-KS-AA-HA (KS482AA, N-terminal 3 x HA)Not specifiedPECO:0000005PMID:119732891389
Cell growth assaymcm6truncated-KS-AA-HA (KS482AA, del aa 864-892, N-terminal 3 x HA)Not specifiedPECO:0000005PMID:11973289
Cell growth assaymcm6ΔNullPMID:7865880
Cell growth assaymcm6-KS-AA (KS482AA)Not specifiedPECO:0000005PMID:11973289
Cell growth assaymcm6-HA (mcm6+, C-terminal 3 x HA)Not specifiedPECO:0000005PMID:11973289
FYPO:0000839inviable elongated mononucleate aseptate cellMicroscopymis5-268PECO:0000004PMID:786588030
FYPO:0000776normal protein phosphorylation23
has_direct_input chk1Western blot assaymcm6-S1 (E824G, D825N)PMID:21971174
FYPO:0000783protein mislocalized to cytoplasm18
affecting mcm7Microscopymis5-268Not specifiedPECO:0000004PMID:11606526
affecting mcm4Microscopymis5-268Not specifiedPECO:0000004PMID:10588642
affecting mcm2Microscopymis5-268Not specifiedPECO:0000004PMID:10588642
FYPO:0000124viable cellCell growth assaymcm6truncated-KR-HA (K482R, del aa 864-892, N-terminal 3 x HA)Not specifiedPECO:0000005PMID:119732893606
Cell growth assaymcm6-KA (K482A)Not specifiedPECO:0000005PMID:11973289
Cell growth assaymcm6truncated-DE-AA-HA (DE540AA, del aa 864-892, N-terminal 3 x HA)Not specifiedPECO:0000005PMID:11973289
Cell growth assaymcm6truncated-KA-HA (K482A, del aa 864-892, N-terminal 3 x HA)Not specifiedPECO:0000005PMID:11973289
Cell growth assaymcm6-DE-AA-HA (DE540AA, N-terminal 3 x HA)Not specifiedPECO:0000005PMID:11973289
Cell growth assaymcm6truncated-DA-HA (D540A, del aa 864-892, N-terminal 3 x HA)Not specifiedPECO:0000005PMID:11973289
Cell growth assaymcm6-DE-AA (DE540AA)Not specifiedPECO:0000005PMID:11973289
Cell growth assaymcm6-KA-HA (K482A, N-terminal 3 x HA)Not specifiedPECO:0000005PMID:11973289
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cdc10 MBF transcription factor complex subunit Cdc10 PMID:10490657
FYPO affected by mutation in mcm2 MCM complex subunit Mcm2 PMID:10588642
FYPO affected by mutation in mcm2 MCM complex subunit Mcm2 PMID:9658174
FYPO affected by mutation in mcm3 MCM complex subunit Mcm3 PMID:10588642
FYPO affected by mutation in mcm4 MCM complex subunit Mcm4/Cdc21 PMID:10588642
FYPO affected by mutation in mcm6 MCM complex subunit Mcm6 PMID:21971174
FYPO affected by mutation in mcm6 MCM complex subunit Mcm6 PMID:9914167
FYPO affected by mutation in rpn12 19S proteasome regulatory subunit Rpn12 PMID:23349636
FYPO affected by mutation in rum1 CDK inhibitor Rum1 PMID:10388806
FYPO affected by mutation in swi1 replication fork protection complex subunit Swi1 PMID:23349636
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
138830653880098
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00493 Pfam IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 414 736 6
PF14551 Pfam IPR027925 MCM N-terminal domain 73 214 6
SM00350 SMART IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 194 737 6
PS50051 Prosite Profiles IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 426 632 6
PS00847 Prosite Patterns IPR018525 Mini-chromosome maintenance, conserved site 535 543 6
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 19 857 1
PTHR11630 HMMPANTHER 19 857 6
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 277 327 48
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 197 224 48
1ltlA01 Gene3D 70 160 6
2.20.28.10 Gene3D IPR004039 Rubredoxin-type fold 226 275 3
3.40.50.300 Gene3D 436 616 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 436 749 294
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 76 328 57
Coil ncoils Rabaptin coiled-coil domain 739 765 968
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 606 614 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 579 591 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 527 541 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 467 482 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 555 568 6
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 195 207 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 355 376 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 140 152 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 245 262 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 298 309 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.60 Da
Charge -27.00
Isoelectric point 4.77
Molecular weight 99.55 kDa
Number of residues 892
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS98PMID:182575171670
present during cellular response to thiabendazole
MOD:00696phosphorylated residueNASPMID:182575171922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2258during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3865during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2493during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2059.12during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1292.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
helicase activity of Mcm4/6/7 complex requires 5' overhang PMID:111362473
processive helicase activity of Mcm4/6/7 complex requires 3' and 5' overhangs PMID:111362473
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
hhf3histone H4 h4.3 Dosage RescuePMID:15507118
bir1survivin, Bir1 Dosage RescuePMID:15507118
cut1separase/separin Dosage RescuePMID:15507118
cig2G1/S-specific B-type cyclin Cig2 Phenotypic SuppressionPMID:21971174
Synthetic Rescue
Synthetic RescuePMID:15194812
cdc23MCM-associated protein Mcm10 Synthetic Growth DefectPMID:12185500
dis1microtubule-associated protein Dis1 Dosage RescuePMID:15507118
rum1CDK inhibitor Rum1 Dosage RescuePMID:15194812
cdc18MCM loader Synthetic RescuePMID:21971174
mcm2MCM complex subunit Mcm2 Dosage Growth DefectPMID:9383050
Dosage RescuePMID:15507118
mcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:11606526
Synthetic LethalityPMID:7865880
alp14TOG ortholog Alp14 Dosage RescuePMID:15507118
hht3histone H3 h3.3 Dosage RescuePMID:15507118
mcm5MCM complex subunit Mcm5 Dosage RescuePMID:15507118
mcb1MCM binding protein homolog Mcb1 (predicted) Dosage RescuePMID:23322785
Synthetic Growth Defect
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic Growth DefectPMID:11027263
mcm7MCM complex subunit Mcm7 Synthetic LethalityPMID:11606526
cdt1replication licensing factor Cdt1 Phenotypic SuppressionPMID:21971174
Synthetic Rescue
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:12006645
Affinity Capture-WesternPMID:15338237
Affinity Capture-WesternPMID:11344166
cdt1replication licensing factor Cdt1 Affinity Capture-WesternPMID:21971174
Two-hybrid
mcm6MCM complex subunit Mcm6 Co-purificationPMID:23444842
cdc23MCM-associated protein Mcm10 Two-hybridPMID:12185500
Two-hybridPMID:22433840
Two-hybridPMID:23695164
bis1stress response protein Bis1 Two-hybridPMID:11751918
mcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:9658174
Affinity Capture-WesternPMID:10770926
Affinity Capture-WesternPMID:21813639
Affinity Capture-WesternPMID:9366552
Co-purification
mcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:11973289
Affinity Capture-WesternPMID:21813639
Affinity Capture-WesternPMID:10770926
Affinity Capture-WesternPMID:9658174
Co-purificationPMID:23444842
Reconstituted ComplexPMID:11136247
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
psf2GINS complex subunit Psf2 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:22433840
mcm5MCM complex subunit Mcm5 Affinity Capture-WesternPMID:9658174
mcb1MCM binding protein homolog Mcb1 (predicted) Affinity Capture-WesternPMID:23322785
Reconstituted Complex
Two-hybrid
Affinity Capture-MSPMID:22036784
Affinity Capture-Western
Affinity Capture-WesternPMID:21813639
sld3DNA replication pre-initiation complex subunit Sld3 Affinity Capture-WesternPMID:12006645
mcm7MCM complex subunit Mcm7 Co-purificationPMID:9705352
Affinity Capture-WesternPMID:21813639
Co-purificationPMID:10770926
Co-purificationPMID:23444842
Affinity Capture-WesternPMID:11606526
Reconstituted ComplexPMID:11136247
External References
Database Identifier Description
NBRP SPBC211.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC211.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC211.04c BioGRID Interaction Datasets
Expression Viewer SPBC211.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC211.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC211.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC211.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC211.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC211.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC211.04c Cell Cycle Data
GEO SPBC211.04c GEO profiles
PInt SPBC211.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC211.04c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN40/40H11Orfeome Localization Data
UniProtKB/SwissProtP49731DNA replication licensing factor mcm6
ModBaseP49731Database of comparative protein structure models
STRINGP49731Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596614MCM complex subunit Mcm6
RefSeq mRNANM_001022535972h- MCM complex subunit Mcm6 (mcm6), mRNA
European Nucleotide ArchiveBAA06729.1ENA Protein Mapping
European Nucleotide ArchiveCAB75412.1ENA Protein Mapping
UniParcUPI000012F162UniProt Archive

Literature for mcm6

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014