mcm6 (SPBC211.04c)


Gene Standard Namemcm6 Characterisation Statuspublished
Systematic IDSPBC211.04c Feature Typeprotein coding
Synonymsmis5 Name Description
ProductMCM complex subunit Mcm6 Product Size892aa, 99.55 kDa
Genomic Location Chromosome II, 3883065-3880098 (2968nt); CDS:3882996-3880318 (2679nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATP-dependent 3'-5' DNA helicase activity11
Annotation ExtensionEvidenceWith/FromReference
ATPase activity236
Annotation ExtensionEvidenceWith/FromReference
single-stranded DNA binding28
Annotation ExtensionEvidenceWith/FromReference
activated by ATP
  • activated by ATP
single-stranded DNA-dependent ATP-dependent DNA helicase activity6
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
double-strand break repair via homologous recombination32
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication72
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication initiation33
Annotation ExtensionEvidenceWith/FromReference
premeiotic DNA replication12
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationmcm6-HA (mcm6+, C-terminal 3 x HA)Not specified1428
mcm6-KS-AA (KS482AA)Not specified
mcm6-KS-AA-HA (KS482AA, N-terminal 3 x HA)Not specified
mcm6ΔNull
mcm6truncated-KS-AA-HA (KS482AA, del aa 864-892, N-terminal 3 x HA)Not specified
unnamed (wild type)
sensitive to camptothecinmcm6-S1 (E824G, D825N)219
sensitive to hydroxyureamis5-268525
sensitive to methyl methanesulfonatemcm6-S1 (E824G, D825N)227
sensitive to UV during vegetative growthmis5-268136
viable vegetative cell populationmcm6-DE-AA (DE540AA)Not specified3783
mcm6-DE-AA-HA (DE540AA, N-terminal 3 x HA)Not specified
mcm6-KA (K482A)Not specified
mcm6-KA-HA (K482A, N-terminal 3 x HA)Not specified
mcm6truncated-DA-HA (D540A, del aa 864-892, N-terminal 3 x HA)Not specified
mcm6truncated-DE-AA-HA (DE540AA, del aa 864-892, N-terminal 3 x HA)Not specified
mcm6truncated-KA-HA (K482A, del aa 864-892, N-terminal 3 x HA)Not specified
mcm6truncated-KR-HA (K482R, del aa 864-892, N-terminal 3 x HA)Not specified

Cell Phenotype

Term NameAlleleExpressionCount
abnormal DNA replicationmis5-26841
abnormal mitotic cell cyclemcm6ΔNull808
abolished protein binding78
affecting mcm6 and cdt1mcm6-S1 (E824G, D825N)
abolished protein localization to nucleus during vegetative growth36
affecting mcm4mis5-268Not specified
cell cycle arrest in mitotic S phasemis5-26810
decreased pre-replicative complex assemblymcm6-S1 (E824G, D825N)1
decreased protein level in germinating spore4
affecting mcm6mcm6Δ::his3+ Null
delayed double-strand break repairmcm6-S1 (E824G, D825N)Endogenous1
elongated aseptate cellmcm6Δ::his3+ Null53
increased duration of mitotic S phasemcm6Δ::his3+ Null13
increased plasmid lossmis5-268Not specified11
inviable after spore germination with elongated germ tubemcm6ΔNull163
inviable after spore germination, single or double cell divisionmcm6ΔNull101
inviable after spore germination, without cell division, cell cycle arrest with replicated DNAmcm6Δ::his3+ Null5
inviable cellmcm6ΔNull1462
inviable elongated mononucleate aseptate cellmis5-26839
normal protein phosphorylation37
affecting chk1mcm6-S1 (E824G, D825N)
protein mislocalized to cytoplasm35
affecting mcm7mis5-268Not specified
affecting mcm2mis5-268Not specified
affecting mcm4mis5-268Not specified
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cdc10 MBF transcription factor complex subunit Cdc10 PMID:10490657
FYPO affected by mutation in mcm2 MCM complex subunit Mcm2 PMID:9658174
FYPO affected by mutation in mcm2 MCM complex subunit Mcm2 PMID:10588642
FYPO affected by mutation in mcm3 MCM complex subunit Mcm3 PMID:10588642
FYPO affected by mutation in mcm4 MCM complex subunit Mcm4/Cdc21 PMID:10588642
FYPO affected by mutation in mcm6 MCM complex subunit Mcm6 PMID:21971174
FYPO affected by mutation in mcm6 MCM complex subunit Mcm6 PMID:9914167
FYPO affected by mutation in rpn12 19S proteasome regulatory subunit Rpn12 PMID:23349636
FYPO affected by mutation in swi1 replication fork protection complex subunit Swi1 PMID:23349636
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
138830653880098

UTRs

Region Coordinates Reference
five_prime_UTR3883065..3882997PMID:21511999
three_prime_UTR3880317..3880098PMID:21511999
exon3882996..3880318
mRNA3883065..3880098
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00493 Pfam IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 414 736 6
PF14551 Pfam IPR027925 MCM N-terminal domain 73 214 6
SM00350 SMART IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 194 737 6
PS50051 Prosite Profiles IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 426 632 6
PS00847 Prosite Patterns IPR018525 Mini-chromosome maintenance, conserved site 535 543 6
PTHR11630 HMMPANTHER 26 882 6
PTHR11630:SF43 HMMPANTHER IPR008049 DNA replication licensing factor Mcm6 26 882 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 436 616 288
1ltlA01 Gene3D 70 160 6
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 277 327 48
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 197 224 48
2.20.28.10 Gene3D IPR004039 Rubredoxin-type fold 226 275 3
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 436 749 294
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 76 328 57
Coil ncoils Rabaptin coiled-coil domain 739 765 968
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 195 207 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 355 376 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 245 262 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 298 309 1
PR01662 PRINTS IPR008049 DNA replication licensing factor Mcm6 140 152 1
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 555 568 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 579 591 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 527 541 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 467 482 6
PR01657 PRINTS IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase 606 614 6

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.60 Da
Charge -27.00
Isoelectric point 4.77
Molecular weight 99.55 kDa
Number of residues 892
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during cellular response to thiabendazoleS98
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S98 PMID:18257517
phosphorylated residue 1923
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelpresent during GO:0051321Western blot evidencePMID:10888871
increased during GO:0006279Western blot evidencePMID:10888871
decreased during GO:0045023Western blot evidencePMID:16899242

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2258during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3865during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2493during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2059.12during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1292.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
helicase activity of Mcm4/6/7 complex requires 5' overhang PMID:111362473
processive helicase activity of Mcm4/6/7 complex requires 3' and 5' overhangs PMID:111362473
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withbis1stress response protein Bis1 Two-hybridPMID:11751918
binds DNA-binding domain construct withcdc23MCM-associated protein Mcm10 Two-hybridPMID:12185500
binds DNA-binding domain construct withcdc23MCM-associated protein Mcm10 Two-hybridPMID:22433840
binds DNA-binding domain construct withcdc23MCM-associated protein Mcm10 Two-hybridPMID:23695164
affinity capturescdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:12006645
affinity captured bycdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:15338237
affinity capturescdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:15338237
affinity captured bycdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:11344166
binds DNA-binding domain construct withcdt1replication licensing factor Cdt1 Two-hybridPMID:21971174
affinity capturescdt1replication licensing factor Cdt1 Affinity Capture-WesternPMID:21971174
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
binds DNA-binding domain construct withmcb1MCM binding protein homolog Mcb1 Two-hybridPMID:23322785
affinity captured bymcb1MCM binding protein homolog Mcb1 Affinity Capture-WesternPMID:23322785
forms complex withmcb1MCM binding protein homolog Mcb1 Reconstituted ComplexPMID:23322785
affinity captured bymcb1MCM binding protein homolog Mcb1 Affinity Capture-MSPMID:22036784
affinity captured bymcb1MCM binding protein homolog Mcb1 Affinity Capture-WesternPMID:22036784
affinity captured bymcb1MCM binding protein homolog Mcb1 Affinity Capture-WesternPMID:21813639
affinity capturesmcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:9658174
affinity captured bymcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:9658174
affinity captured bymcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:10770926
affinity captured bymcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:21813639
affinity captured bymcm2MCM complex subunit Mcm2 Affinity Capture-WesternPMID:9366552
co-purifies withmcm2MCM complex subunit Mcm2 Co-purificationPMID:9366552
affinity captured bymcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:11973289
affinity captured bymcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:21813639
affinity captured bymcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:10770926
affinity captured bymcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:9658174
affinity capturesmcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:9658174
co-purifies withmcm4MCM complex subunit Mcm4/Cdc21 Co-purificationPMID:23444842
forms complex withmcm4MCM complex subunit Mcm4/Cdc21 Reconstituted ComplexPMID:11136247
affinity capturesmcm5MCM complex subunit Mcm5 Affinity Capture-WesternPMID:9658174
co-purifies withmcm6MCM complex subunit Mcm6 Co-purificationPMID:23444842
co-purifies withmcm7MCM complex subunit Mcm7 Co-purificationPMID:9705352
affinity captured bymcm7MCM complex subunit Mcm7 Affinity Capture-WesternPMID:21813639
co-purifies withmcm7MCM complex subunit Mcm7 Co-purificationPMID:10770926
co-purifies withmcm7MCM complex subunit Mcm7 Co-purificationPMID:23444842
affinity captured bymcm7MCM complex subunit Mcm7 Affinity Capture-WesternPMID:11606526
forms complex withmcm7MCM complex subunit Mcm7 Reconstituted ComplexPMID:11136247
affinity captured bypsf2GINS complex subunit Psf2 Affinity Capture-WesternPMID:22718908
affinity captured bypsf2GINS complex subunit Psf2 Affinity Capture-WesternPMID:22433840
affinity captured bysld3DNA replication pre-initiation complex subunit Sld3 Affinity Capture-WesternPMID:12006645
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofalp14TOG/XMAP14 family protein Alp14 Dosage RescuePMID:15507118
rescued by overexpression ofbir1survivin, Bir1 Dosage RescuePMID:15507118
synthetically rescued bycdc18MCM loader Synthetic RescuePMID:21971174
synthetic growth defect withcdc23MCM-associated protein Mcm10 Synthetic Growth DefectPMID:12185500
rescued bycdt1replication licensing factor Cdt1 Phenotypic SuppressionPMID:21971174
synthetically rescued bycdt1replication licensing factor Cdt1 Synthetic RescuePMID:21971174
rescued bycig2G1/S-specific B-type cyclin Cig2 Phenotypic SuppressionPMID:21971174
synthetically rescued bycig2G1/S-specific B-type cyclin Cig2 Synthetic RescuePMID:21971174
synthetically rescued bycig2G1/S-specific B-type cyclin Cig2 Synthetic RescuePMID:15194812
rescued by overexpression ofcut1separase/separin Dosage RescuePMID:15507118
rescued by overexpression ofdis1TOG/XMAP14 microtubule-associated protein Dis1 Dosage RescuePMID:15507118
rescued by overexpression ofhhf3histone H4 h4.3 Dosage RescuePMID:15507118
rescued by overexpression ofhht3histone H3 h3.3 Dosage RescuePMID:15507118
synthetic growth defect withhsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic Growth DefectPMID:11027263
synthetic growth defect withmcb1MCM binding protein homolog Mcb1 Synthetic Growth DefectPMID:23322785
overexpression rescuesmcb1MCM binding protein homolog Mcb1 Dosage RescuePMID:23322785
overexpression causes growth defect tomcm2MCM complex subunit Mcm2 Dosage Growth DefectPMID:9383050
rescued by overexpression ofmcm2MCM complex subunit Mcm2 Dosage RescuePMID:15507118
synthetic lethal withmcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:11606526
synthetic lethal withmcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:7865880
rescued by overexpression ofmcm5MCM complex subunit Mcm5 Dosage RescuePMID:15507118
synthetic lethal withmcm7MCM complex subunit Mcm7 Synthetic LethalityPMID:11606526
rescued by overexpression ofrum1CDK inhibitor Rum1 Dosage RescuePMID:15194812
External References
Database Identifier Description
NBRP SPBC211.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC211.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC211.04c BioGRID Interaction Datasets
Expression Viewer SPBC211.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC211.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC211.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC211.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC211.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC211.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC211.04c Cell Cycle Data
GEO SPBC211.04c GEO profiles
PInt SPBC211.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC211.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC211.04c Fission yeast phenotypic data & analysis
SPD / RIKEN40/40H11Orfeome Localization Data
UniProtKB/SwissProtP49731DNA replication licensing factor mcm6
ModBaseP49731Database of comparative protein structure models
STRINGP49731Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596614MCM complex subunit Mcm6
RefSeq mRNANM_001022535972h- MCM complex subunit Mcm6 (mcm6), mRNA
European Nucleotide ArchiveBAA06729.1ENA Protein Mapping
European Nucleotide ArchiveCAB75412.1ENA Protein Mapping
UniParcUPI000012F162UniProt Archive

Literature for mcm6

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015