mtr3 (SPBC211.08c)

Gene Standard Namemtr3 Characterisation Statuspublished
Systematic IDSPBC211.08c Feature Typeprotein coding
Synonyms Name Description
Productexosomal 3'-5' exoribonuclease, PH-like Mtr3 Product Size257aa, 28.44 kDa
Genomic Location Chromosome II, 3888596-3886905 (1692nt); CDS:3888278-3887467 (812nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
3'-5'-exoribonuclease activity17
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasmic exosome (RNase complex)10
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
exosome (RNase complex)14
Annotation ExtensionEvidenceWith/FromReference
nuclear exosome (RNase complex)13
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationmtr3Δ1452

Cell Phenotype

Term NameGenotypesCount
inviable vegetative cell, abnormal cell shape, normal cell sizemtr3Δ199
Target Of
FYPO affected by mutation in mtl1 TRAMP complex ATP-dependent RNA helicase (predicted)
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3888596..3888164, 3888125..3886905
5' UTR3888596..3888279PMID:21511999
CDS3888278..3888164, 3888125..3887467
3' UTR3887466..3886905PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01138 Pfam IPR001247 Exoribonuclease, phosphorolytic domain 1 35 162 6
PTHR11953:SF2 HMMPANTHER 10 248 1
PTHR11953 HMMPANTHER 10 248 3
1e3hA01 Gene3D IPR027408 PNPase/RNase PH domain 26 193 4
SSF54211 SuperFamily IPR020568 Ribosomal protein S5 domain 2-type fold 30 162 29
SSF55666 SuperFamily IPR015847 Exoribonuclease, phosphorolytic domain 2 166 247 5

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.64 Da
Charge 3.00
Codon Adaptation Index 0.33
Isoelectric point 7.74
Molecular weight 28.44 kDa
Number of residues 257
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for mtr3 (SPBC211.08c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5070during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
5931during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
5672during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
4836during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
5218during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
3620.43during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
2119.54during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.5during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.94during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC211.08c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withrrp41exosomal 3'-to-5' phosphorolytic exoribonuclease Rrp41 Two-hybridPMID:26771498
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured byrrp43exosome subunit Rrp43 Affinity Capture-MSPMID:23503588
affinity captured bydis33'-5' exoribonuclease subunit Dis3 Affinity Capture-MSPMID:23503588
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
External References
Database Identifier Description
NBRP SPBC211.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC211.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC211.08c BioGRID Interaction Datasets
Expression Viewer SPBC211.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC211.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC211.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC211.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC211.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC211.08c Transcriptome Viewer (Bähler Lab)
GEO SPBC211.08c GEO profiles
PInt SPBC211.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC211.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC211.08c Fission yeast phenotypic data & analysis
Cyclebase SPBC211.08c.1 Cell Cycle Data
SPD / RIKEN10/10D03Orfeome Localization Data
IntEnz3.1.13.-Integrated relational Enzyme database
Rhea3.1.13.-Annotated reactions database
UniProtKB/SwissProtQ9P7R3Exosome complex component mtr3
ModBaseQ9P7R3Database of comparative protein structure models
STRINGQ9P7R3Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596618exosome complex component mtr3
RefSeq mRNANM_001022539972h- exosome complex component mtr3 (SPBC211.08c), mRNA
European Nucleotide ArchiveCAB75416.1ENA Protein Mapping
UniParcUPI000006AFF3UniProt Archive

Literature for mtr3

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016