SPBC215.01


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPBC215.01 Feature Typeprotein coding
SynonymsSPBC3B9.20 Name Description
ProductGTPase activating protein (predicted) Product Size834aa, 95.01 kDa
Genomic Location Chromosome II, 4024162-4027252 (3091nt); CDS:4024572-4027076 (2505nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium ion binding39
Annotation ExtensionEvidenceWith/FromReference
GTPase activator activity42
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
regulation of GTPase activity76
Annotation ExtensionEvidenceWith/FromReference
signal transduction402
Annotation ExtensionEvidenceWith/FromReference
vesicle-mediated transport323
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to cadmiumSPBC215.01ΔNull246
sensitive to hydroxyureaSPBC215.01ΔNull525
viable vegetative cell populationSPBC215.01ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologySPBC215.01ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4024162..4027252
mRNA4024162..4027252
5' UTR4024162..4024571PMID:21511999
CDS4024572..4027076
3' UTR4027077..4027252PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00566 Pfam IPR000195 Rab-GTPase-TBC domain 224 428 13
SM00164 SMART IPR000195 Rab-GTPase-TBC domain 217 431 13
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 625 637 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 589 601 12
PS50086 Prosite Profiles IPR000195 Rab-GTPase-TBC domain 220 408 13
PS50222 Prosite Profiles IPR002048 EF-hand domain 576 611 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 612 647 20
PTHR22957:SF148 HMMPANTHER 60 812 2
PTHR22957 HMMPANTHER 60 812 11
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 507 673 20
2qq8A02 Gene3D 214 332 12
SSF47923 SuperFamily IPR000195 Rab-GTPase-TBC domain 192 368 13
SSF47923 SuperFamily IPR000195 Rab-GTPase-TBC domain 344 445 13
SSF47473 SuperFamily 497 671 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 113.92 Da
Charge -9.00
Isoelectric point 5.67
Molecular weight 95.01 kDa
Number of residues 834
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS807
present during mitotic M phaseS774
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S774 PMID:21712547
present during mitotic M phase experimental evidence S807 PMID:21712547
O-phospho-L-threonine 698
present during mitotic M phaseT809
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T809 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2686during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3089during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2937during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3445during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3009during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
372.56during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withatg13autophagy associated protein kinase regulatory subunit Atg13 Negative GeneticPMID:22681890
negative genetic interaction withcbh1CENP-B homolog Cbh1 Negative GeneticPMID:22681890
negative genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
positive genetic interaction withhus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
positive genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
negative genetic interaction withmkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
positive genetic interaction withmug80cyclin Clg1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withpho24-nitrophenylphosphatase Negative GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction withppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withppk8serine/threonine protein kinase Ppk8 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
negative genetic interaction withSPBP8B7.18cphosphomethylpyrimidine kinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
negative genetic interaction withssm4dynactin microtubule-binding subunit, p150-Glued Ssm4 Negative GeneticPMID:22681890
negative genetic interaction withste20Rictor homolog, Ste20 Negative GeneticPMID:22681890
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
negative genetic interaction withsum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
negative genetic interaction withswd3WD repeat protein Swd3 Negative GeneticPMID:22681890
negative genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtps1alpha,alpha-trehalose-phosphate synthase Negative GeneticPMID:22681890
negative genetic interaction withtsc2tuberin Negative GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
negative genetic interaction withvps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC215.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC215.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC215.01 BioGRID Interaction Datasets
Expression Viewer SPBC215.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC215.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC215.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC215.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC215.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC215.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC215.01 Cell Cycle Data
GEO SPBC215.01 GEO profiles
PInt SPBC215.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC215.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC215.01 Fission yeast phenotypic data & analysis
SPD / RIKEN29/29B02Orfeome Localization Data
UniProtKB/SwissProtO43048TBC domain-containing protein C215.01
ModBaseO43048Database of comparative protein structure models
STRINGO43048Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596678GTPase activating protein (predicted)
RefSeq mRNANM_001022600972h- GTPase activating protein (predicted) (SPBC215.01), mRNA
European Nucleotide ArchiveCAA17800.2ENA Protein Mapping
UniParcUPI000006C89FUniProt Archive

Literature for SPBC215.01

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015