SPBC215.01


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPBC215.01 Feature Typeprotein coding
SynonymsSPBC3B9.20 Name Description
ProductGTPase activating protein (predicted) Product Size834aa, 95.01 kDa
Genomic Location Chromosome II, 4024162-4027252 (3091nt); CDS:4024572-4027076 (2505nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium ion binding48
Annotation ExtensionEvidenceWith/FromReference
Rab GTPase activator activity13
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
regulation of Rab GTPase activity20
Annotation ExtensionEvidenceWith/FromReference
signal transduction394
Annotation ExtensionEvidenceWith/FromReference
vesicle-mediated transport320
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site301
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000003Cell growth assaySPBC215.01ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000088sensitive to hydroxyureaCell growth assaySPBC215.01ΔNullPECO:0000137, PECO:0000005PMID:23173672514
FYPO:0002060viable vegetative cell populationMicroscopySPBC215.01ΔNullPMID:204732893759
MicroscopySPBC215.01ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001MicroscopySPBC215.01ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
140241624027252

UTRs

Region Coordinates Reference
five_prime_UTR4024162..4024571PMID:21511999
three_prime_UTR4027077..4027252PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00566 Pfam IPR000195 Rab-GTPase-TBC domain 224 428 13
SM00164 SMART IPR000195 Rab-GTPase-TBC domain 217 431 13
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 625 637 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 589 601 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 576 611 20
PS50086 Prosite Profiles IPR000195 Rab-GTPase-TBC domain 220 408 13
PS50222 Prosite Profiles IPR002048 EF-hand domain 612 647 20
PTHR22957 HMMPANTHER 59 549 12
PTHR22957:SF94 HMMPANTHER 59 549 2
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 507 673 20
2qq8A02 Gene3D 214 332 12
SSF47923 SuperFamily IPR000195 Rab-GTPase-TBC domain 344 445 13
SSF47923 SuperFamily IPR000195 Rab-GTPase-TBC domain 192 368 13
SSF47473 SuperFamily 497 671 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Protein Properties

Ave. residue weight 113.92 Da
Charge -9.00
Isoelectric point 5.67
Molecular weight 95.01 kDa
Number of residues 834
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS807PMID:217125471670
present during mitotic M phase
present during mitotic M phaseexperimental evidenceS774PMID:21712547
MOD:00047O-phospho-L-threonineexperimental evidenceT809PMID:21712547692
present during mitotic M phase
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2686during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3089during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2937during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3445during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
372.56during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3009during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
ppk8serine/threonine protein kinase Ppk8 (predicted) Negative GeneticPMID:22681890
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
vps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
swd3WD repeat protein Swd3 Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
ssm4dynactin microtubule-binding subunit, p150-Glued Ssm4 Negative GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
ste20Rictor homolog, Ste20 Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
mkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
mug80cyclin Clg1 (predicted) Positive GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
ppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
cbh1CENP-B homolog Cbh1 Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
atg13autophagy associated protein kinase regulatory subunit Atg13 Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
SPBP8B7.18cphosphomethylpyrimidine kinase (predicted) Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
tps1alpha,alpha-trehalose-phosphate synthase Negative GeneticPMID:22681890
vps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
pho24-nitrophenylphosphatase Negative GeneticPMID:22681890
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
tsc2tuberin Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC215.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC215.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC215.01 BioGRID Interaction Datasets
Expression Viewer SPBC215.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC215.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC215.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC215.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC215.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC215.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC215.01 Cell Cycle Data
GEO SPBC215.01 GEO profiles
PInt SPBC215.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC215.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC215.01 Fission yeast phenotypic data & analysis
SPD / RIKEN29/29B02Orfeome Localization Data
UniProtKB/SwissProtO43048TBC domain-containing protein C215.01
ModBaseO43048Database of comparative protein structure models
STRINGO43048Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596678GTPase activating protein (predicted)
RefSeq mRNANM_001022600972h- GTPase activating protein (predicted) (SPBC215.01), mRNA
European Nucleotide ArchiveCAA17800.2ENA Protein Mapping
UniParcUPI000006C89FUniProt Archive

Literature for SPBC215.01

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014