rfc1 (SPBC23E6.07c)

Gene Standard Namerfc1 Characterisation Statuspublished
Systematic IDSPBC23E6.07c Feature Typeprotein coding
Synonyms Name Description
ProductDNA replication factor C complex subunit Rfc1 Product Size934aa, 103.49 kDa
Genomic Location Chromosome II, 3856111-3852657 (3455nt); CDS:3855942-3852962 (2981nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
DNA clamp loader activity11
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

DNA replication

Term NameCount
leading strand elongation3
Annotation ExtensionEvidenceWith/FromReference
mitotic cell cycle DNA replication69
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Ctf18 RFC-like complex8
Annotation ExtensionEvidenceWith/FromReference
Elg1 RFC-like complex6
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin283
Annotation ExtensionEvidenceWith/FromReference
nuclear DNA replication factor C complex6
Annotation ExtensionEvidenceWith/FromReference
nuclear replication fork64
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Rad17 RFC-like complex6
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyrfc1ΔNullPECO:0000005, PECO:0000137PMID:236978061338
Microscopynot recorded (unrecorded)PMID:16040599

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopyrfc1ΔNullPECO:0000005, PECO:0000137PMID:23697806686
FYPO:0001122elongated vegetative cellMicroscopyrfc1ΔNullPMID:11313445685
FYPO:0003529inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells87
penetrance FYPO_EXT:0000001Microscopyrfc1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00004 Pfam IPR003959 ATPase, AAA-type, core 412 520 32
PF00533 Pfam IPR001357 BRCT domain 237 313 8
PF08519 Pfam IPR013725 DNA replication factor RFC1, C-terminal 688 843 1
SM00382 SMART IPR003593 AAA+ ATPase domain 408 542 70
SM00292 SMART IPR001357 BRCT domain 238 316 10
PS50172 Prosite Profiles IPR001357 BRCT domain 236 310 13
PTHR23389:SF0 HMMPANTHER 1 934 2
PTHR23389 HMMPANTHER 1 934 3 Gene3D 541 611 35 Gene3D 359 532 288 Gene3D 613 732 6 Gene3D IPR001357 BRCT domain 236 314 13
SSF48019 SuperFamily IPR008921 DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal 612 760 6
SSF52113 SuperFamily IPR001357 BRCT domain 235 314 12
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 359 603 294
PIRSF036578 PIRSF IPR012178 DNA replication factor C, large subunit 1 932 1
Coil ncoils Rabaptin coiled-coil domain 326 347 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000443BRCT domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000443
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539

Protein Properties

Ave. residue weight 110.80 Da
Charge 30.00
Isoelectric point 9.62
Molecular weight 103.49 kDa
Number of residues 934

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS42PMID:217125471670
present during mitotic M phase
present during mitotic M phaseexperimental evidenceS887PMID:21712547
experimental evidenceS42PMID:24763107
present during mitotic M phaseexperimental evidenceS885PMID:21712547
present during mitotic M phaseexperimental evidenceS22PMID:21712547
present during mitotic M phaseexperimental evidenceS27PMID:21712547
MOD:00047O-phospho-L-threonineexperimental evidenceT24PMID:21712547692
present during mitotic M phase
present during mitotic M phaseexperimental evidenceT26PMID:21712547
MOD:00696phosphorylated residueNASPMID:195477441922
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4814during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
4491during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
4530during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
4145during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
3262.17during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
4893during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
2403.74during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.91during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
cdc24DNA replication protein Cdc24 Affinity Capture-WesternPMID:9891039
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rfc5DNA replication factor C complex subunit Rfc5 (predicted) Synthetic RescuePMID:16040599
dcc1Ctf18 RFC-like complex subunit Dcc1 Synthetic LethalityPMID:16040599
cdc24DNA replication protein Cdc24 Dosage RescuePMID:9891039
elg1DNA replication factor C complex subunit Elg1 Dosage LethalityPMID:16040599
ctf18RFC-like complex subunit Ctf18 Synthetic LethalityPMID:16040599
External References
Database Identifier Description
NBRP SPBC23E6.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC23E6.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC23E6.07c BioGRID Interaction Datasets
Expression Viewer SPBC23E6.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC23E6.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC23E6.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC23E6.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC23E6.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC23E6.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC23E6.07c Cell Cycle Data
GEO SPBC23E6.07c GEO profiles
PInt SPBC23E6.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC23E6.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC23E6.07c Fission yeast phenotypic data & analysis
SPD / RIKEN29/29G10Orfeome Localization Data
UniProtKB/SwissProtO60182Replication factor C subunit 1
ModBaseO60182Database of comparative protein structure models
STRINGO60182Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596607DNA replication factor C complex subunit Rfc1
RefSeq mRNANM_001022528972h- DNA replication factor C complex subunit Rfc1 (rfc1), mRNA
European Nucleotide ArchiveBAA87235.1ENA Protein Mapping
European Nucleotide ArchiveCAA18875.1ENA Protein Mapping
UniParcUPI000006C6D3UniProt Archive

Literature for rfc1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014