SPBC25B2.01


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPBC25B2.01 Feature Typeprotein coding
SynonymsSPBC2G5.08 Name Description
Productelongation factor 1 alpha related protein (predicted) Product Size592aa, 66.02 kDa
Genomic Location Chromosome II, 2594358-2596452 (2095nt); CDS:2594464-2596339 (1876nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005525GTP bindingIEAUniProtKB-KW:KW-0342GO_REF:0000037113
GO:0003924GTPase activityIEAIPR000795GO_REF:000000261
GO:0003746translation elongation factor activityIEAUniProtKB-KW:KW-0251GO_REF:000003715
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002182cytoplasmic translational elongationNASGO_REF:0000051193
GO:0006417regulation of translationIEAUniProtKB-KW:KW-0810GO_REF:000003766
GO:0006412translationISOSGD:S000001792GO_REF:0000024599
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000001792GO_REF:00000244204
GO:0005829cytosolIDAPMID:168233722319
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopySPBC25B2.01ΔNullPMID:204732893751
MicroscopySPBC25B2.01ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001MicroscopySPBC25B2.01ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
125943582594503
225945582594611
325946552596452
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03144 Pfam IPR004161 Translation elongation factor EFTu/EF1A, domain 2 419 486 16
PF03143 Pfam IPR004160 Translation elongation factor EFTu/EF1A, C-terminal 492 591 6
PF00009 Pfam IPR000795 Elongation factor, GTP-binding domain 176 396 18
PF08938 Pfam IPR015033 HBS1-like protein, N-terminal 11 84 1
PTHR23115:SF35 HMMPANTHER 156 591 1
PTHR23115 HMMPANTHER 156 591 17
3.40.50.300 Gene3D 170 395 288
2.40.30.10 Gene3D 402 505 29
2.40.30.10 Gene3D 506 591 29
SSF50465 SuperFamily IPR009001 Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal 496 591 7
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 173 416 294
SSF50447 SuperFamily IPR009000 Translation protein, beta-barrel domain 396 491 24
PR00315 PRINTS IPR000795 Elongation factor, GTP-binding domain 318 327 15
PR00315 PRINTS IPR000795 Elongation factor, GTP-binding domain 179 192 15
PR00315 PRINTS IPR000795 Elongation factor, GTP-binding domain 238 246 15
PR00315 PRINTS IPR000795 Elongation factor, GTP-binding domain 258 268 15
PR00315 PRINTS IPR000795 Elongation factor, GTP-binding domain 274 285 15

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.51 Da
Charge -8.00
Isoelectric point 5.37
Molecular weight 66.02 kDa
Number of residues 592
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15778during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
15854during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
15413during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
17447during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3867.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
14631during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
680.28during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.4during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.46during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
elp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
SPCC1840.09NAD dependent epimerase/dehydratase family protein Negative GeneticPMID:22681890
arg3ornithine carbamoyltransferase Arg3 Negative GeneticPMID:22681890
atp14F1-ATPase subunit H (predicted) Negative GeneticPMID:22681890
vps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
SPCC1919.05Ski complex TPR repeat subunit Ski3 (predicted) Negative GeneticPMID:22681890
liz1pantothenate transporter Liz1 Negative GeneticPMID:22681890
cgi121EKC/KEOPS complex subunit Cgi121 (predicted) Positive GeneticPMID:22681890
SPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
SPAPB1E7.11cSchizosaccharomyces specific protein Negative GeneticPMID:22681890
atp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
erg28Erg28 protein (predicted) Negative GeneticPMID:22681890
atp10F1-F0 ATPase assembly protein (predicted) Negative GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
SPBC25H2.15SSU-rRNA maturation protein Tsr4 homolog 1 (predicted) Negative GeneticPMID:22681890
hop1linear element associated protein Hop1 Negative GeneticPMID:22681890
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
ppr5mitochondrial PPR repeat protein Ppr5 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
dom34peloto ortholog (predicted) Reconstituted ComplexPMID:20890290
Co-crystal or NMR structure
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBC25B2.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC25B2.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC25B2.01 BioGRID Interaction Datasets
Expression Viewer SPBC25B2.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC25B2.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC25B2.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC25B2.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC25B2.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC25B2.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC25B2.01 Cell Cycle Data
GEO SPBC25B2.01 GEO profiles
PInt SPBC25B2.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC25B2.01 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN26/26D02Orfeome Localization Data
UniProtKB/SwissProtO74774Elongation factor 1 alpha-like protein
ModBaseO74774Database of comparative protein structure models
STRINGO74774Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713085972h- elongation factor 1 alpha related protein (predicted) (SPBC25B2.01), mRNA
RefSeq PeptideXP_001713137elongation factor 1 alpha related protein (predicted)
ePDB3MCAThe European PDB
PDB3MCAPDB
PDBsum3MCAPDBsum
European Nucleotide ArchiveCAA21259.2ENA Protein Mapping
UniParcUPI000006C873UniProt Archive

Literature for SPBC25B2.01

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014