hbs1 (SPBC25B2.01)


Gene Standard Namehbs1 Characterisation Statusbiological role inferred
Systematic IDSPBC25B2.01 Feature Typeprotein coding
SynonymsSPBC2G5.08 Name Description
ProductDom34-Hbs1 translation release factor complex subunit Hbs1 (predicted) Product Size592aa, 66.02 kDa
Genomic Location Chromosome II, 2594358-2596452 (2095nt); CDS:2594464-2596339 (1876nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding88
Annotation ExtensionEvidenceWith/FromReference
translation elongation factor activity16
Annotation ExtensionEvidenceWith/FromReference
translation release factor activity7
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translational elongation194
Annotation ExtensionEvidenceWith/FromReference
cytoplasmic translational termination6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4212
Annotation ExtensionEvidenceWith/FromReference
cytosol2298
Annotation ExtensionEvidenceWith/FromReference
Dom34-Hbs1 complex2
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationhbs1Δ3862

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyhbs1Δ3103
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2594358..2594503, 2594558..2594611, 2594655..2596452
Intron2594504..2594557, 2594612..2594654
mRNA2594358..2596452
5' UTR2594358..2594463AU013219
CDS2594464..2594503, 2594558..2594611, 2594655..2596339
3' UTR2596340..2596452PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00009 Pfam Transcription factor, GTP-binding domain 176 396 16
PF03143 Pfam IPR004160 Translation elongation factor EFTu/EF1A, C-terminal 492 591 6
PF08938 Pfam IPR015033 HBS1-like protein, N-terminal 13 80 1
PS51722 Prosite Profiles IPR000795 Transcription factor, GTP-binding domain 175 401 17
PTHR23115:SF188 HMMPANTHER 162 591 2
PTHR23115 HMMPANTHER 162 591 17
2.40.30.10 Gene3D Translation initiation factor 2, gamma subunit 402 505 29
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 170 395 288
2.40.30.10 Gene3D Translation initiation factor 2, gamma subunit 506 591 29
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 173 416 294
SSF50465 SuperFamily IPR009001 Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal 496 591 7
SSF50447 SuperFamily IPR009000 Translation protein, beta-barrel domain 396 491 24
PR00315 PRINTS IPR000795 Transcription factor, GTP-binding domain 238 246 15
PR00315 PRINTS IPR000795 Transcription factor, GTP-binding domain 179 192 15
PR00315 PRINTS IPR000795 Transcription factor, GTP-binding domain 258 268 15
PR00315 PRINTS IPR000795 Transcription factor, GTP-binding domain 274 285 15
PR00315 PRINTS IPR000795 Transcription factor, GTP-binding domain 318 327 15

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.51 Da
Charge -8.00
Codon Adaptation Index 0.38
Isoelectric point 5.37
Molecular weight 66.02 kDa
Number of residues 592
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS100 2293
Annotation ExtensionEvidenceResidueReference
IDA S100 PMID:25720772
phosphorylated residue 2515
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for hbs1 (SPBC25B2.01)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15778during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
15854during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
15413during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
17447during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14631during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3867.2during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
680.28during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.4during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.46during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in bacteria1005
conserved in metazoa3498
conserved in vertebrates3473
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC25B2.01 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withdom34Dom34-Hbs1 translation release factor complex subunit, peloto ortholog Dom34 (predicted) Reconstituted ComplexPMID:20890290
co-locates (via crystalisation or NMR) withdom34Dom34-Hbs1 translation release factor complex subunit, peloto ortholog Dom34 (predicted) Co-crystal or NMR structurePMID:20890290
binds DNA-binding domain construct withhal3thymidylate synthase/ flavoprotein fusion protein Hal3 Two-hybridPMID:26771498
binds DNA-binding domain construct withmcp5cortical anchoring factor for dynein Mcp5/Num1 Two-hybridPMID:26771498
binds activation domain construct withdom34Dom34-Hbs1 translation release factor complex subunit, peloto ortholog Dom34 (predicted) Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC25B2.01 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction witherg28Erg28 protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC25H2.15SSU-rRNA maturation protein Tsr4 homolog 1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsum2translation initiation inhibitor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcoq11ubiquinone biosynthesis protein Coq11 Negative GeneticPMID:22681890
negative genetic interaction witharg3ornithine carbamoyltransferase Arg3 Negative GeneticPMID:22681890
negative genetic interaction withski3Ski complex TPR repeat subunit Ski3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
negative genetic interaction withatp14F1-ATPase subunit H (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhop1linear element associated protein Hop1 Negative GeneticPMID:22681890
negative genetic interaction withatp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
negative genetic interaction withppr5mitochondrial PPR repeat protein Ppr5 Negative GeneticPMID:22681890
negative genetic interaction withSPAPB1E7.11cSchizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withvps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
negative genetic interaction withatp10mitochondrial F1-F0 ATPase assembly protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withliz1pantothenate transmembrane transporter Liz1 Negative GeneticPMID:22681890
positive genetic interaction withcgi121EKC/KEOPS complex subunit Cgi121 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withelp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC25B2.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC25B2.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC25B2.01 BioGRID Interaction Datasets
Expression Viewer SPBC25B2.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC25B2.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC25B2.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC25B2.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC25B2.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC25B2.01 Transcriptome Viewer (Bähler Lab)
GEO SPBC25B2.01 GEO profiles
PInt SPBC25B2.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC25B2.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC25B2.01 Fission yeast phenotypic data & analysis
Cyclebase SPBC25B2.01.1 Cell Cycle Data
SPD / RIKEN26/26D02Orfeome Localization Data
UniProtKB/SwissProtO74774Elongation factor 1 alpha-like protein
ModBaseO74774Database of comparative protein structure models
STRINGO74774Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713085972h- elongation factor 1 alpha related protein (predicted) (SPBC25B2.01), mRNA
RefSeq PeptideXP_001713137elongation factor 1 alpha related protein (predicted)
ePDB3MCAThe European PDB
PDB3MCAPDB
PDBsum3MCAPDBsum
European Nucleotide ArchiveCAA21259.2ENA Protein Mapping
UniParcUPI000006C873UniProt Archive

Literature for hbs1

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017