pof2 (SPBC25B2.11)


Gene Standard Namepof2 Characterisation Statuspublished
Systematic IDSPBC25B2.11 Feature Typeprotein coding
Synonyms Name Description
ProductF-box protein Pof2 Product Size463aa, 52.36 kDa
Genomic Location Chromosome II, 2617862-2619476 (1615nt); CDS:2617862-2619336 (1475nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding863
Annotation ExtensionEvidenceWith/FromReference
protein binding, bridging27
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
protein modification by small protein conjugation or removal188
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrion752
Annotation ExtensionEvidenceWith/FromReference
ubiquitin ligase complex67
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationpof2Δ3837

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologypof2Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2617862..2619052, 2619089..2619228, 2619276..2619476
Intron2619053..2619088, 2619229..2619275
3' UTR2619337..2619476PMID:18641648
3' UTR2619337..2619476PMID:20118936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12937 Pfam IPR001810 F-box domain 3 44 7
SM00367 SMART IPR006553 Leucine-rich repeat, cysteine-containing subtype 93 118 2
SM00367 SMART IPR006553 Leucine-rich repeat, cysteine-containing subtype 276 301 2
SM00367 SMART IPR006553 Leucine-rich repeat, cysteine-containing subtype 302 327 2
SM00367 SMART IPR006553 Leucine-rich repeat, cysteine-containing subtype 171 196 2
SM00367 SMART IPR006553 Leucine-rich repeat, cysteine-containing subtype 380 405 2
SM00367 SMART IPR006553 Leucine-rich repeat, cysteine-containing subtype 328 353 2
SM00367 SMART IPR006553 Leucine-rich repeat, cysteine-containing subtype 354 378 2
SM00367 SMART IPR006553 Leucine-rich repeat, cysteine-containing subtype 145 170 2
PS50181 Prosite Profiles IPR001810 F-box domain 1 42 10
PTHR24006 HMMPANTHER 1 34 18
PTHR24006 HMMPANTHER 74 420 18
3.80.10.10 Gene3D IPR032675 Leucine-rich repeat domain, L domain-like 2 246 16
3.80.10.10 Gene3D IPR032675 Leucine-rich repeat domain, L domain-like 250 421 16
SSF52047 SuperFamily 114 371 6
SSF81383 SuperFamily IPR001810 F-box domain 3 91 12

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000933F-box proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000933
PBO:0001527leucine-rich repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001527

Protein Properties

Ave. residue weight 113.09 Da
Charge 10.50
Codon Adaptation Index 0.36
Isoelectric point 8.09
Molecular weight 52.36 kDa
Number of residues 463
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pof2 (SPBC25B2.11)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.74during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.34during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC25B2.11 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withSPAC15A10.06CPA1 sodium ion/proton antiporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmaa1mitochondrial aspartate aminotransferase Maa1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withnem1Nem1-Spo7 phosphatase complex catalytic subunit Nem1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpex6peroxin-6 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withset1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC56E4.03aromatic aminotransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharv1Arv1-like family protein Arv1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withzwf2glucose-6-phosphate 1-dehydrogenase Zwf2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpfl4cell surface glycoprotein, adhesion molecule (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC23C4.09cDNA-binding TFAR19-related protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtma20RNA-binding protein Tma20 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhop1linear element associated protein Hop1 Negative GeneticPMID:22681890
negative genetic interaction withpvg2galactose residue biosynthesis protein Pvg2 Negative GeneticPMID:22681890
negative genetic interaction withdph2diphthamide biosynthesis protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC25H2.03vacuolar protein involved in phosphoinositide metabolism (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC1795.10cSed5 Vesicle Protein Svp26 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmug113T5orf172 family protein Negative GeneticPMID:22681890
negative genetic interaction withpam17TIM23 translocase complex-associated motor subunit Pam17 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
negative genetic interaction withmac1membrane anchored protein Mac1 Negative GeneticPMID:22681890
negative genetic interaction withget4ER membrane insertion protein Get4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withlaf1Clr6 L associated factor 1 Laf1 Negative GeneticPMID:22681890
negative genetic interaction withvps25ESCRT II complex subunit Vps25 Negative GeneticPMID:22681890
positive genetic interaction withmed13mediator complex subunit Med13 Positive GeneticPMID:22681890
positive genetic interaction withmug178mitochondrial ribosomal protein subunit L51-b (predicted) Positive GeneticPMID:22681890
positive genetic interaction withppr2mitochondrial PPR repeat protein Ppr2 Positive GeneticPMID:22681890
phenotype enhanced byppk38Ark1/Prk1 family protein kinase Ppk38 Phenotypic EnhancementPMID:19547744
phenotype enhanced byubp3ubiquitin C-terminal hydrolase Ubp3 Phenotypic EnhancementPMID:19547744
External References
Database Identifier Description
NBRP SPBC25B2.11 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC25B2.11 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC25B2.11 BioGRID Interaction Datasets
Expression Viewer SPBC25B2.11 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC25B2.11 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC25B2.11 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC25B2.11 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC25B2.11 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC25B2.11 Transcriptome Viewer (Bähler Lab)
GEO SPBC25B2.11 GEO profiles
PInt SPBC25B2.11 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC25B2.11 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC25B2.11 Fission yeast phenotypic data & analysis
Cyclebase SPBC25B2.11.1 Cell Cycle Data
SPD / RIKEN44/44G08Orfeome Localization Data
UniProtKB/SwissProtO74783SCF E3 ubiquitin ligase complex F-box protein pof2
ModBaseO74783Database of comparative protein structure models
STRINGO74783Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596079F-box protein Pof2
RefSeq mRNANM_001021991972h- F-box protein Pof2 (pof2), mRNA

Literature for pof2

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016