dnt1 (SPBC25D12.02c)


Gene Standard Namednt1 Characterisation Statuspublished
Systematic IDSPBC25D12.02c Feature Typeprotein coding
Synonyms Name Description
Productnucleolar protein Dnt1 Product Size599aa, 65.98 kDa
Genomic Location Chromosome II, 3717257-3714945 (2313nt); CDS:3717051-3715200 (1852nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding887
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
chromatin silencing at rDNA9
Annotation ExtensionEvidenceWith/FromReference
negative regulation of protein localization to mitotic spindle pole body1
Annotation ExtensionEvidenceWith/FromReference
negative regulation of septation initiation signaling17
Annotation ExtensionEvidenceWith/FromReference
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of G2/M transition of mitotic cell cycle19
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle271
Annotation ExtensionEvidenceWith/FromReference
nucleolus365
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationdnt1Δ3850

Cell Phenotype

Term NameGenotypesCount
decreased protein localization to mitotic spindle pole body during telophase1
affects localization of dma1dnt1Δ
increased protein level during vegetative growth114
affecting wee1dnt1Δ
increased protein localization to mitotic spindle pole body during metaphase3
affects localization of dma1dnt1Δ
increased protein phosphorylation during vegetative growth65
affecting cdc2dnt1Δ
increased protein ubiquitination5
affecting sid4dnt1Δ
increased ubiquitin ligase activity during mitotic metaphase1
affecting dma1dnt1Δ
mitotic G2/M transition delaydnt1Δ40
normal protein level during vegetative growth91
affecting dma1dnt1Δ
normal protein localization to mitotic spindle pole body during G21
affecting dma1dnt1Δ
viable elongated vegetative celldnt1Δ325
viable vegetative cell with normal cell morphologydnt1Δ3102

FYPO Multi-allele Phenotypes

Cell Phenotype

Term NameGenotypes
viable small vegetative cellcdc2-1w (G146D), dnt1Δ
wee1-50, dnt1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in dma1 mitotic spindle checkpoint ubiquitin ligase Dma1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3717257..3716909, 3716856..3714945
Intron3716908..3716857
mRNA3717257..3714945
5' UTR3717257..3717052PMID:21511999
CDS3717051..3716909, 3716856..3715200
3' UTR3715199..3714945PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10407 Pfam IPR018844 Nucleolar protein Dnt1-like, N-terminal 17 81 1
PTHR28196 HMMPANTHER 1 599 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.15 Da
Charge -26.00
Codon Adaptation Index 0.37
Isoelectric point 4.65
Molecular weight 65.98 kDa
Number of residues 599
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS515
present during mitotic M phaseS276
present during mitotic M phaseS590
present during mitotic M phaseS522
present during mitotic M phaseS395
present during mitotic M phaseS575
present during mitotic M phaseS194
S156, S172, S174, S175, S244, S245, S247, S248, S258, S276, S395, S470, S503, S515, S590
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S515 PMID:21712547
present during mitotic M phase experimental evidence S276 PMID:21712547
present during mitotic M phase experimental evidence S590 PMID:21712547
present during mitotic M phase experimental evidence S522 PMID:21712547
present during mitotic M phase experimental evidence S395 PMID:21712547
present during mitotic M phase experimental evidence S575 PMID:21712547
present during mitotic M phase experimental evidence S194 PMID:21712547
IDA S174 PMID:25720772
IDA S247 PMID:25720772
IDA S470 PMID:25720772
IDA S175 PMID:25720772
experimental evidence S590 PMID:24763107
IDA S590 PMID:25720772
IDA S515 PMID:25720772
IDA S245 PMID:25720772
experimental evidence S395 PMID:24763107
IDA S244 PMID:25720772
IDA S248 PMID:25720772
experimental evidence S515 PMID:24763107
IDA S503 PMID:25720772
IDA S156 PMID:25720772
IDA S258 PMID:25720772
experimental evidence S276 PMID:24763107
IDA S276 PMID:25720772
IDA S172 PMID:25720772
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:22809626
IDA PMID:19547744
O-phosphorylated residueS172,S174, S172,S175, S172,S181, S244,S245, S244,S248, S245,S247, S245,S248, S245,S249, S247,S248, S248,S249, S257,S258, S294,S297, S306,S316,S317, S311,S316,S317, S503,S508, T255,S260, T501,Y504, Y171,S174, Y171,S175 2457
Annotation ExtensionEvidenceResidueReference
IDA S311,S316,S317 PMID:25720772
IDA S172,S175 PMID:25720772
IDA Y171,S175 PMID:25720772
IDA S257,S258 PMID:25720772
IDA S245,S247 PMID:25720772
IDA T501,Y504 PMID:25720772
IDA S172,S174 PMID:25720772
IDA S244,S248 PMID:25720772
IDA T255,S260 PMID:25720772
IDA S245,S249 PMID:25720772
IDA S247,S248 PMID:25720772
IDA S245,S248 PMID:25720772
IDA Y171,S174 PMID:25720772
IDA S294,S297 PMID:25720772
IDA S244,S245 PMID:25720772
IDA S172,S181 PMID:25720772
IDA S503,S508 PMID:25720772
IDA S248,S249 PMID:25720772
IDA S306,S316,S317 PMID:25720772
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelconstant during GO:0000278Western blot evidencePMID:22809626

Quantitative Gene Expression

View graphical display of gene expression data for dnt1 (SPBC25D12.02c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5302during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4400during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6110during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3992during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3844during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2696.74during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3844.61during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi only616
conserved in fungi4607
conserved in eukaryotes4517
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC25D12.02c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypmt3SUMO Affinity Capture-MSPMID:22730331
affinity captured bydma1mitotic spindle checkpoint ubiquitin ligase Dma1 Affinity Capture-MSPMID:22809626
binds DNA-binding domain construct withclp1Cdc14-related protein phosphatase Clp1/Flp1 Two-hybridPMID:17538026
binds DNA-binding domain construct withdma1mitotic spindle checkpoint ubiquitin ligase Dma1 Two-hybridPMID:22809626
affinity capturesdma1mitotic spindle checkpoint ubiquitin ligase Dma1 Affinity Capture-MSPMID:22809626
forms complex withdma1mitotic spindle checkpoint ubiquitin ligase Dma1 Reconstituted ComplexPMID:22809626
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC25D12.02c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withplo1Polo kinase Plo1 Synthetic Growth DefectPMID:22809626
synthetic growth defect withrrn5RNA polymerase I upstream activation factor complex subunit Rrn5 Synthetic Growth DefectPMID:17538026
synthetically rescuescdc14SIN component Cdc14 Synthetic RescuePMID:17538026
synthetically rescuesclp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic RescuePMID:17538026
synthetically rescuessid2NDR kinase Sid2 Synthetic RescuePMID:17538026
synthetically rescuesmyo2myosin II heavy chain Synthetic RescuePMID:17538026
synthetically rescuessid1PAK-related GC kinase Sid1 Synthetic RescuePMID:17538026
rescued bydma1mitotic spindle checkpoint ubiquitin ligase Dma1 Phenotypic SuppressionPMID:22809626
synthetic lethal withcdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic LethalityPMID:24006256
synthetic lethal withnuc1DNA-directed RNA polymerase I complex large subunit Nuc1 Synthetic LethalityPMID:17538026
synthetically rescued bydma1mitotic spindle checkpoint ubiquitin ligase Dma1 Synthetic RescuePMID:22809626
phenotype enhanced bydma1mitotic spindle checkpoint ubiquitin ligase Dma1 Phenotypic EnhancementPMID:22809626
External References
Database Identifier Description
NBRP SPBC25D12.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC25D12.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC25D12.02c BioGRID Interaction Datasets
Expression Viewer SPBC25D12.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC25D12.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC25D12.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC25D12.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC25D12.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC25D12.02c Transcriptome Viewer (Bähler Lab)
GEO SPBC25D12.02c GEO profiles
PInt SPBC25D12.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC25D12.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC25D12.02c Fission yeast phenotypic data & analysis
Cyclebase SPBC25D12.02c.1 Cell Cycle Data
SPD / RIKEN46/46A05Orfeome Localization Data
UniProtKB/SwissProtO74354Nucleolar protein dnt1
ModBaseO74354Database of comparative protein structure models
STRINGO74354Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596544nucleolar protein Dnt1
RefSeq mRNANM_001022465972h- nucleolar protein Dnt1 (dnt1), mRNA
European Nucleotide ArchiveCAA20098.1ENA Protein Mapping
UniParcUPI0000069C20UniProt Archive

Literature for dnt1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016