dnt1 (SPBC25D12.02c)


Gene Standard Namednt1 Characterisation Statuspublished
Systematic IDSPBC25D12.02c Feature Typeprotein coding
Synonyms Name Description
Productnucleolar protein Dnt1 Product Size599aa, 65.98 kDa
Genomic Location Chromosome II, 3717257-3714945 (2313nt); CDS:3717051-3715200 (1852nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPIclp1PMID:17538026861
during mitotic anaphaseIPIdma1PMID:22809626
during mitotic metaphaseIPIdma1PMID:22809626
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000183chromatin silencing at rDNAIMPPMID:1753802610
GO:1902543negative regulation of protein localization to mitotic spindle pole body1
during mitotic metaphase, regulates dma1IMPPMID:22809626
GO:0031030negative regulation of septation initiation signaling15
different_pathwayIGIclp1PMID:17538026
GO:0051436negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle2
regulates dma1IMPPMID:22809626
GO:0010971positive regulation of G2/M transition of mitotic cell cycle9
independent of pom1IMPPMID:24006256
independent of cut12IMPPMID:24006256
independent of cds1IMPPMID:24006256
independent of clp1IMPPMID:24006256
independent of rad3IMPPMID:24006256
independent of chk1IMPPMID:24006256
independent of cdc25IMPPMID:24006256
dependent on wee1IMPPMID:24006256
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:168233724201
GO:0072686mitotic spindleIDAPMID:22809626217
GO:0005730nucleolusIDAPMID:22809626355
constitutiveIDAPMID:17538026
GO:0005819spindle275
during mitotic cell cycleIDAPMID:17538026
during mitotic anaphase BIDAPMID:17538026
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopydnt1ΔNullPECO:0000005, PECO:0000137PMID:236978063760
Microscopydnt1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002971decreased protein localization to mitotic spindle pole body during telophase1
affecting dma1Microscopydnt1ΔNullPMID:22809626
FYPO:0001327increased protein level during vegetative growth35
affecting wee1Western blot assaydnt1ΔNullPMID:24006256
FYPO:0002970increased protein localization to mitotic spindle pole body during metaphase1
affecting dma1Microscopydnt1ΔNullPMID:22809626
FYPO:0001038increased protein phosphorylation during vegetative growth19
affecting cdc2Western blot assaydnt1ΔNullPMID:24006256
FYPO:0002082increased protein ubiquitination5
affecting sid4Western blot assaydnt1ΔNullPMID:22809626
FYPO:0002973increased ubiquitin ligase activity during mitotic metaphase1
affecting dma1Western blot assaydnt1ΔNullPMID:22809626
FYPO:0000012mitotic G2/M transition delayMicroscopydnt1ΔNullPMID:2400625615
FYPO:0000833normal protein level32
affecting dma1Western blot assaydnt1ΔNullPMID:22809626
FYPO:0002968normal protein localization to mitotic spindle pole body during G21
affecting dma1Microscopydnt1ΔNullPMID:22809626
FYPO:0001492viable elongated vegetative cellMicroscopydnt1ΔNullPMID:24006256182
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopydnt1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in dma1 mitotic spindle checkpoint ubiquitin ligase Dma1 PMID:22809626
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
137172573716909
237168563714945

UTRs

Region Coordinates Reference
five_prime_UTR3717257..3717052PMID:21511999
three_prime_UTR3715199..3714945PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10407 Pfam IPR018844 Nucleolar protein Dnt1-like, N-terminal 15 83 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.15 Da
Charge -26.00
Isoelectric point 4.65
Molecular weight 65.98 kDa
Number of residues 599
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS395PMID:217125471663
present during mitotic M phase
present during mitotic M phaseS276PMID:21712547
S395PMID:24763107
S276PMID:24763107
S590PMID:24763107
S515PMID:24763107
present during mitotic M phaseS194PMID:21712547
present during mitotic M phaseS575PMID:21712547
present during mitotic M phaseS590PMID:21712547
present during mitotic M phaseS522PMID:21712547
present during mitotic M phaseS515PMID:21712547
MOD:00696phosphorylated residueIDAPMID:228096261915
NASPMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelconstant during GO:0000278Western blot evidencePMID:22809626

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
5302during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4400during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
2696.74during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
3844.61during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
3844during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
6110during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3992during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
1.9during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi only603
conserved in fungi4569
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic RescuePMID:17538026
myo2myosin II heavy chain Synthetic RescuePMID:17538026
rrn5RNA polymerase I upstream activation factor complex subunit Rrn5 Synthetic Growth DefectPMID:17538026
sid2NDR kinase Sid2 Synthetic RescuePMID:17538026
Synthetic RescuePMID:24006256
nuc1DNA-directed RNA polymerase I complex large subunit Nuc1 Synthetic LethalityPMID:17538026
plo1Polo kinase Plo1 Synthetic Growth DefectPMID:22809626
cdc14SIN component Cdc14 Synthetic RescuePMID:17538026
Synthetic RescuePMID:24006256
cdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic LethalityPMID:24006256
dma1mitotic spindle checkpoint ubiquitin ligase Dma1 Synthetic RescuePMID:22809626
Phenotypic Suppression
Phenotypic Enhancement
sid1PAK-related GC kinase Sid1 Synthetic RescuePMID:17538026
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Two-hybridPMID:17538026
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
dma1mitotic spindle checkpoint ubiquitin ligase Dma1 Two-hybridPMID:22809626
Affinity Capture-MS
Affinity Capture-Western
Reconstituted Complex
External References
Database Identifier Description
NBRP SPBC25D12.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC25D12.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC25D12.02c BioGRID Interaction Datasets
Expression Viewer SPBC25D12.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC25D12.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC25D12.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC25D12.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC25D12.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC25D12.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC25D12.02c Cell Cycle Data
GEO SPBC25D12.02c GEO profiles
PInt SPBC25D12.02c Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2540499nucleolar protein Dnt1
WikiGene2540499nucleolar protein Dnt1
SPD / RIKEN46/46A05Orfeome Localization Data
UniProtKB/SwissProtO74354Nucleolar protein dnt1
ModBaseO74354Database of comparative protein structure models
STRINGO74354Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596544nucleolar protein Dnt1
RefSeq mRNANM_001022465972h- nucleolar protein Dnt1 (dnt1), mRNA
European Nucleotide ArchiveCAA20098ENA Protein Mapping
European Nucleotide ArchiveCAA20098.1ENA Protein Mapping
UniParcUPI0000069C20UniProt Archive

Literature for dnt1

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014