dnt1 (SPBC25D12.02c)


Gene Standard Namednt1 Characterisation Statuspublished
Systematic IDSPBC25D12.02c Feature Typeprotein coding
Synonyms Name Description
Productnucleolar protein Dnt1 Product Size599aa, 65.98 kDa
Genomic Location Chromosome II, 3717257-3714945 (2313nt); CDS:3717051-3715200 (1852nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
chromatin silencing at rDNA10
Annotation ExtensionEvidenceWith/FromReference
negative regulation of protein localization to mitotic spindle pole body1
Annotation ExtensionEvidenceWith/FromReference
negative regulation of septation initiation signaling15
Annotation ExtensionEvidenceWith/FromReference
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle3
Annotation ExtensionEvidenceWith/FromReference
positive regulation of G2/M transition of mitotic cell cycle12
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4206
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle258
Annotation ExtensionEvidenceWith/FromReference
nucleolus359
Annotation ExtensionEvidenceWith/FromReference
spindle278
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationdnt1ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
decreased protein localization to mitotic spindle pole body during telophase1
affecting dma1dnt1ΔNull
increased protein level during vegetative growth72
affecting wee1dnt1ΔNull
increased protein localization to mitotic spindle pole body during metaphase3
affecting dma1dnt1ΔNull
increased protein phosphorylation during vegetative growth31
affecting cdc2dnt1ΔNull
increased protein ubiquitination5
affecting sid4dnt1ΔNull
increased ubiquitin ligase activity during mitotic metaphase1
affecting dma1dnt1ΔNull
mitotic G2/M transition delaydnt1ΔNull29
normal protein level during vegetative growth47
affecting dma1dnt1ΔNull
normal protein localization to mitotic spindle pole body during G21
affecting dma1dnt1ΔNull
viable elongated vegetative celldnt1ΔNull231
viable vegetative cell with normal cell morphology3092
penetrance FYPO_EXT:0000001dnt1ΔNull
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in dma1 mitotic spindle checkpoint ubiquitin ligase Dma1 PMID:22809626
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
137172573716909
237168563714945

UTRs

Region Coordinates Reference
five_prime_UTR3717257..3717052PMID:21511999
three_prime_UTR3715199..3714945PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10407 Pfam IPR018844 Nucleolar protein Dnt1-like, N-terminal 15 83 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.15 Da
Charge -26.00
Isoelectric point 4.65
Molecular weight 65.98 kDa
Number of residues 599
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS276
present during mitotic M phaseS575
present during mitotic M phaseS590
S276, S395, S515, S590
present during mitotic M phaseS522
present during mitotic M phaseS515
present during mitotic M phaseS194
present during mitotic M phaseS395
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S194 PMID:21712547
experimental evidence S276 PMID:24763107
present during mitotic M phase experimental evidence S276 PMID:21712547
experimental evidence S395 PMID:24763107
present during mitotic M phase experimental evidence S395 PMID:21712547
experimental evidence S515 PMID:24763107
present during mitotic M phase experimental evidence S515 PMID:21712547
present during mitotic M phase experimental evidence S522 PMID:21712547
present during mitotic M phase experimental evidence S575 PMID:21712547
experimental evidence S590 PMID:24763107
present during mitotic M phase experimental evidence S590 PMID:21712547
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
IDA PMID:22809626
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelconstant during GO:0000278Western blot evidencePMID:22809626

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5302during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4400during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6110during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3992during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2696.74during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3844during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3844.61during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only619
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Two-hybridPMID:17538026
dma1mitotic spindle checkpoint ubiquitin ligase Dma1 Affinity Capture-MSPMID:22809626
Two-hybrid
Reconstituted Complex
Affinity Capture-Western
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic RescuePMID:17538026
myo2myosin II heavy chain Synthetic RescuePMID:17538026
rrn5RNA polymerase I upstream activation factor complex subunit Rrn5 Synthetic Growth DefectPMID:17538026
sid2NDR kinase Sid2 Synthetic RescuePMID:17538026
Synthetic RescuePMID:24006256
nuc1DNA-directed RNA polymerase I complex large subunit Nuc1 Synthetic LethalityPMID:17538026
plo1Polo kinase Plo1 Synthetic Growth DefectPMID:22809626
cdc14SIN component Cdc14 Synthetic RescuePMID:17538026
Synthetic RescuePMID:24006256
sid1PAK-related GC kinase Sid1 Synthetic RescuePMID:17538026
dma1mitotic spindle checkpoint ubiquitin ligase Dma1 Phenotypic EnhancementPMID:22809626
Phenotypic Suppression
Synthetic Rescue
cdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic LethalityPMID:24006256
External References
Database Identifier Description
NBRP SPBC25D12.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC25D12.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC25D12.02c BioGRID Interaction Datasets
Expression Viewer SPBC25D12.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC25D12.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC25D12.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC25D12.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC25D12.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC25D12.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC25D12.02c Cell Cycle Data
GEO SPBC25D12.02c GEO profiles
PInt SPBC25D12.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC25D12.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC25D12.02c Fission yeast phenotypic data & analysis
SPD / RIKEN46/46A05Orfeome Localization Data
UniProtKB/SwissProtO74354Nucleolar protein dnt1
ModBaseO74354Database of comparative protein structure models
STRINGO74354Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596544nucleolar protein Dnt1
RefSeq mRNANM_001022465972h- nucleolar protein Dnt1 (dnt1), mRNA
European Nucleotide ArchiveCAA20098.1ENA Protein Mapping
UniParcUPI0000069C20UniProt Archive

Literature for dnt1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015