pph3 (SPBC26H8.05c)


Gene Standard Namepph3 Characterisation Statusbiological role inferred
Systematic IDSPBC26H8.05c Feature Typeprotein coding
Synonyms Name Description
Productserine/threonine protein phosphatase, PP4 complex subunit Pph3 (predicted) Product Size348aa, 39.22 kDa
Genomic Location Chromosome II, 3956123-3954552 (1572nt); CDS:3955929-3954715 (1215nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding749
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine phosphatase activity16
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

signaling


Term NameCount
protein dephosphorylation42
Annotation ExtensionEvidenceWith/FromReference
signal transduction361
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2691
Annotation ExtensionEvidenceWith/FromReference
protein phosphatase 4 complex2
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencypph3Δ272
increased cell population growth ratepph3Δ256
viable vegetative cell populationpph3Δ3844

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologypph3Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3956123..3955860, 3955744..3955290, 3955236..3954552
Intron3955859..3955745, 3955289..3955237
mRNA3956123..3954552
5' UTR3956123..3955930PMID:21511999
CDS3955929..3955860, 3955744..3955290, 3955236..3954715
3' UTR3954714..3954552PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00149 Pfam IPR004843 Calcineurin-like phosphoesterase domain, apaH type 47 238 20
SM00156 SMART IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 19 331 10
PS00125 Prosite Patterns IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 110 115 10
PTHR11668 HMMPANTHER 294 348 11
PTHR11668 HMMPANTHER 1 252 11
PTHR11668:SF24 HMMPANTHER 294 348 2
PTHR11668:SF24 HMMPANTHER 1 252 2
3.60.21.10 Gene3D IPR029052 Metallo-dependent phosphatase-like 292 341 23
3.60.21.10 Gene3D IPR029052 Metallo-dependent phosphatase-like 3 255 23
SSF56300 SuperFamily IPR029052 Metallo-dependent phosphatase-like 293 331 22
SSF56300 SuperFamily IPR029052 Metallo-dependent phosphatase-like 3 256 22
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 76 103 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 229 249 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 173 200 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 109 133 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 47 74 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 293 309 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 144 170 10

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.69 Da
Charge -17.50
Codon Adaptation Index 0.40
Isoelectric point 4.44
Molecular weight 39.22 kDa
Number of residues 348
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pph3 (SPBC26H8.05c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2020.63during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.8during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.72during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC26H8.05c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byimp1importin alpha Affinity Capture-MSPMID:25963819
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC26H8.05c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withpds5mitotic and meiotic cohesin-associated protein Pds5 Negative GeneticPMID:18818364
negative genetic interaction withmsy1MS calcium ion channel protein Msy1 Negative GeneticPMID:22681890
negative genetic interaction withers1RNA-silencing factor Ers1 Negative GeneticPMID:18818364
negative genetic interaction withrpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:18818364
negative genetic interaction withraf2Rik1-associated factor Raf2 Negative GeneticPMID:18818364
negative genetic interaction withmrc1mediator of replication checkpoint 1 Negative GeneticPMID:18818364
negative genetic interaction withrsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
negative genetic interaction witharb1argonaute inhibitor protein 1 Negative GeneticPMID:22681890
negative genetic interaction withswi6HP1 family chromodomain protein Swi6 Negative GeneticPMID:22681890
negative genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
negative genetic interaction withreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:18818364
negative genetic interaction withapq12nuclear membrane organization protein Apq12 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:18818364
negative genetic interaction withSPCC4F11.03cSchizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withfin1serine/threonine protein kinase, NIMA related Fin1 Negative GeneticPMID:18818364
negative genetic interaction withdcr1dicer Negative GeneticPMID:18818364
negative genetic interaction withrik1silencing protein Rik1 Negative GeneticPMID:18818364
negative genetic interaction withrpl160360S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withchp1chromodomain protein Chp1 Negative GeneticPMID:18818364
negative genetic interaction withrdp1RNA-directed RNA polymerase Rdp1 Negative GeneticPMID:18818364
negative genetic interaction withcwf21complexed with Cdc5 protein Cwf21 Negative GeneticPMID:18818364
negative genetic interaction withalp14TOG/XMAP14 family protein Alp14 Negative GeneticPMID:18818364
negative genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:18818364
negative genetic interaction withepe1Jmjc domain chromatin associated protein Epe1 Negative GeneticPMID:18818364
negative genetic interaction withfep1iron-sensing transcription factor Fep1 Negative GeneticPMID:22681890
negative genetic interaction withcho1phosphatidyl-N-dimethylethanolamine N-methyltransferase Negative GeneticPMID:18818364
negative genetic interaction withSPBC29A10.16ccytochrome b5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC1393.08transcription factor, zf-GATA type (predicted) Negative GeneticPMID:22681890
negative genetic interaction withamo1nuclear rim protein Amo1 Negative GeneticPMID:18818364
negative genetic interaction withdop1Dopey family protein Dop1 Negative GeneticPMID:22681890
negative genetic interaction withmed6mediator complex subunit Med6 Negative GeneticPMID:18818364
negative genetic interaction withscs7sphingosine hydroxylase Scs7 Negative GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withcbs2AMP-activated protein kinase gamma subunit cbs2 Negative GeneticPMID:18818364
negative genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:18818364
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:18818364
positive genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Positive GeneticPMID:21504829
positive genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Positive GeneticPMID:18818364
positive genetic interaction withaca1L-azetidine-2-carboxylic acid acetyltransferase Aca1 Positive GeneticPMID:22681890
positive genetic interaction withcaa1cytoplasmic aspartate aminotransferase Caa1 (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC26H8.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC26H8.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC26H8.05c BioGRID Interaction Datasets
Expression Viewer SPBC26H8.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC26H8.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC26H8.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC26H8.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC26H8.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC26H8.05c Transcriptome Viewer (Bähler Lab)
GEO SPBC26H8.05c GEO profiles
PInt SPBC26H8.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC26H8.05c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC26H8.05c Fission yeast phenotypic data & analysis
Cyclebase SPBC26H8.05c.1 Cell Cycle Data
SPD / RIKEN22/22B06Orfeome Localization Data
UniProtKB/SwissProtO74789Putative serine/threonine-protein phosphatase C26H8.05c
ModBaseO74789Database of comparative protein structure models
STRINGO74789Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596646serine/threonine protein phosphatase (predicted)
RefSeq mRNANM_001022568972h- serine/threonine protein phosphatase (predicted) (SPBC26H8.05c), mRNA
European Nucleotide ArchiveCAA21097.1ENA Protein Mapping
UniParcUPI000006BE2AUniProt Archive

Literature for pph3

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016