dis3 (SPBC26H8.10)


Gene Standard Namedis3 Characterisation Statuspublished
Systematic IDSPBC26H8.10 Feature Typeprotein coding
Synonymsrrp44 Name Descriptionchromosome DISjunction,
defective in sister chromatid disjoining
Product3'-5' exoribonuclease subunit Dis3 Product Size970aa, 110.14 kDa
Genomic Location Chromosome II, 3970898-3974218 (3321nt); CDS:3970969-3974045 (3077nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
3'-5'-exoribonuclease activity16
Annotation ExtensionEvidenceWith/FromReference
endoribonuclease activity36
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
Ran GTPase binding15
Annotation ExtensionEvidenceWith/FromReference
ribonuclease activity57
Annotation ExtensionEvidenceWith/FromReference
RNA binding646
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)15
Annotation ExtensionEvidenceWith/FromReference
exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)1
Annotation ExtensionEvidenceWith/FromReference
mitotic sister chromatid segregation192
Annotation ExtensionEvidenceWith/FromReference
ncRNA catabolic process19
Annotation ExtensionEvidenceWith/FromReference
nonfunctional rRNA decay13
Annotation ExtensionEvidenceWith/FromReference
nuclear polyadenylation-dependent mRNA catabolic process12
Annotation ExtensionEvidenceWith/FromReference
nuclear polyadenylation-dependent rRNA catabolic process12
Annotation ExtensionEvidenceWith/FromReference
nuclear polyadenylation-dependent tRNA catabolic process12
Annotation ExtensionEvidenceWith/FromReference
nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay17
Annotation ExtensionEvidenceWith/FromReference
nuclear-transcribed mRNA catabolic process, non-stop decay15
Annotation ExtensionEvidenceWith/FromReference
positive regulation of Ran GTPase activity2
Annotation ExtensionEvidenceWith/FromReference
RNA phosphodiester bond hydrolysis, endonucleolytic24
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasmic exosome (RNase complex)10
Annotation ExtensionEvidenceWith/FromReference
exosome (RNase complex)14
Annotation ExtensionEvidenceWith/FromReference
nuclear exosome (RNase complex)13
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaydis3-54 (P509L)Not specifiedPECO:0000006, PECO:0000201, PECO:0000102PMID:3409871147
FYPO:0001355decreased vegetative cell population growthCell growth assaydis3-54 (P509L)Not specifiedPECO:0000005PMID:20129053381
FYPO:0002061inviable vegetative cell populationMicroscopydis3ΔNullPECO:0000005, PECO:0000137PMID:236978061338
Cell growth assaydis3-54 (P509L)Not specifiedPECO:0000006PMID:20129053
Microscopydis3ΔNullPMID:20473289
FYPO:0001164normal growth on glucose carbon sourceCell growth assaydis3-54 (P509L)Not specifiedPECO:0000102, PECO:0000004PMID:12376568176
FYPO:0000097sensitive to caffeineCell growth assaydis3-54 (P509L)Not specifiedPECO:0000201, PECO:0000227, PECO:0000004PMID:3409871105
FYPO:0000080sensitive to coldCell growth assaydis3-54 (P509L)Not specifiedPECO:0000102PMID:1237656843
FYPO:0000091sensitive to thiabendazoleCell growth assaydis3-54 (P509L)Not specifiedPMID:3409871176
Cell growth assaydis3-54 (P509L)Not specifiedPECO:0000006PMID:17380189
Cell growth assaydis3-54 (P509L)Not specifiedPECO:0000005PMID:17380189

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000209abnormal attachment of spindle microtubules to kinetochore involved in homologous chromosome segregationMicroscopydis3-54 (P509L)Not specifiedPECO:0000006PMID:173801891
FYPO:0000392abnormal cell cycle arrest at mitotic metaphase/anaphase transitionMicroscopydis3-54 (P509L)Not specifiedPECO:0000006PMID:173801891
FYPO:0000059abnormal mitotic cell cycleMicroscopydis3ΔNullPECO:0000005, PECO:0000137PMID:23697806686
FYPO:0000671abnormal rDNA separationMicroscopydis3-54 (P509L)Not specifiedPECO:0000006PMID:34098713
FYPO:0001946abolished mitotic chromosome separationMicroscopydis3-54 (P509L)Not specifiedPECO:0000006PMID:1738018912
FYPO:0003217decreased chromatin silencing at centromere central coreReporter gene assaydis3-54 (P509L)EndogenousPECO:0000006PMID:173801894
FYPO:0003411decreased chromatin silencing at centromere inner repeatReporter gene assaydis3-54 (P509L)EndogenousPECO:0000006PMID:173801892
FYPO:0003412decreased chromatin silencing at centromere outer repeatReporter gene assaydis3-54 (P509L)Not specifiedPECO:0000006PMID:173801893
FYPO:0002827decreased chromatin silencing at silent mating-type cassetteReporter gene assaydis3-54 (P509L)EndogenousPECO:0000006PMID:1738018910
FYPO:0003822decreased ribonuclease activity1
expressivity FYPO_EXT:0000001Enzyme assay datadis3-RNB (T808A)EndogenousPECO:0000006PMID:17380189
Enzyme assay datadis3-54 (P509L)EndogenousPECO:0000006PMID:17380189
Enzyme assay datadis3-54 (P509L)EndogenousPECO:0000005PMID:17380189
Enzyme assay datadis3-RNB (T808A)EndogenousPECO:0000005PMID:17380189
FYPO:0002638increased activation of mitotic cell cycle spindle assembly checkpointMicroscopydis3-54 (P509L)Not specifiedPECO:0000006PMID:173801894
FYPO:0000220increased centromeric outer repeat transcript levelTranscript expression level evidencedis3-54 (P509L)Not specifiedPECO:0000006PMID:1738018916
FYPO:0001840increased minichromosome loss during vegetative growthMicroscopydis3-54 (P509L)Not specifiedPECO:0000004, PECO:0000227PMID:340987134
FYPO:0003003increased protein localization to kinetochore3
affecting bub1Microscopydis3-54 (P509L)Not specifiedPECO:0000006PMID:17380189
affecting mad2Microscopydis3-54 (P509L)Not specifiedPECO:0000006PMID:17380189
FYPO:0000314inviable after spore germination with elongated germ tubeMicroscopydis3ΔNullPECO:0000005, PECO:0000137PMID:23697806163
FYPO:0001042inviable after spore germination, single or double cell divisionMicroscopydis3ΔNullPECO:0000005, PECO:0000137PMID:2369780697
FYPO:0000049inviable cellEnzyme assay datadis3::LEU2NullPMID:19442661449
FYPO:0000733long mitotic spindleMicroscopydis3-54 (P509L)Not specifiedPECO:0000006PMID:173801896
FYPO:0002688mitotic spindle collapseMicroscopydis3-54 (P509L)Not specifiedPECO:0000006PMID:173801894
FYPO:0002837normal centromeric outer repeat transcript-derived siRNA levelTranscript expression level evidencedis3-54 (P509L)EndogenousPECO:0000006PMID:173801892
FYPO:0001761normal mitotic G1/S phase transitionFlow cytometry datadis3-54 (P509L)Not specifiedPECO:0000006PMID:19442663
FYPO:0001759normal protein phosphatase activityEnzyme assay datadis3-54 (P509L)Not specifiedPECO:0000006PMID:19442664
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in mtl1 TRAMP complex ATP-dependent RNA helicase (predicted) PMID:24713849
FYPO affected by mutation in ned1 lipin Ned1 PMID:12376568
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
139708983971161
239713263974218

UTRs

Region Coordinates Reference
five_prime_UTR3970898..3970968PMID:21511999
three_prime_UTR3974046..3974218PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13638 Pfam IPR002716 PIN domain 86 209 2
PF00773 Pfam 496 828 5
SM00955 SMART 496 829 5
SM00670 SMART IPR002716 PIN domain 83 198 5
PS01175 Prosite Patterns IPR022966 Ribonuclease II/R, conserved site 796 820 2
PS50126 Prosite Profiles IPR003029 Ribosomal protein S1, RNA-binding domain 869 969 6
PTHR23355 HMMPANTHER 18 969 5
PTHR23355:SF13 HMMPANTHER 18 969 1
3.40.50.1010 Gene3D 83 216 9
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 878 962 57
SSF88723 SuperFamily PIN domain-like 83 217 10
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 245 336 57
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 393 441 57
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 450 873 57

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001933PINc domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001933

Protein Properties

Ave. residue weight 113.54 Da
Charge 5.50
Isoelectric point 6.88
Molecular weight 110.14 kDa
Number of residues 970
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7301during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6084during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6637during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7097during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6679during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6514.75during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1625.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
oligomeric PMID:172082574
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1802510578
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
rmn1RNA-binding protein Affinity Capture-MSPMID:24713849
iss10Mmi1-driven selective elimination factor Iss10 Affinity Capture-MSPMID:24713849
Affinity Capture-MSPMID:24210919
pab2poly(A) binding protein Pab2 Affinity Capture-WesternPMID:20129053
ski7ski complex interacting GTPase Ski7 Affinity Capture-MSPMID:23503588
rrp43exosome subunit Rrp43 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
dis33'-5' exoribonuclease subunit Dis3 Affinity Capture-MSPMID:23503588
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
rrp40exosome subunit Rrp40 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
mtr3exosomal 3'-5' exoribonuclease, PH-like Mtr3 Affinity Capture-MSPMID:23503588
csl4exosome subunit Csl4 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
rrp4exosome subunit Rrp4 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
act1actin Act1 Affinity Capture-MSPMID:23755256
rrp6exosome 3'-5' exoribonuclease subunit Rrp6 (predicted) Affinity Capture-MSPMID:23503588
cti1Cut3 interacting protein Cti1, predicted exosome subunit Affinity Capture-MSPMID:23503588
rrp41exosomal 3'-to-5' phosphorolytic exoribonuclease Rrp41 Affinity Capture-MSPMID:23503588
rrp46exosome subunit Rrp46 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
red1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
Affinity Capture-MSPMID:24210919
rrp42exosome subunit Rrp42 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
mtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
spi1Ran GTPase Spi1 Co-fractionationPMID:8896453
Affinity Capture-Western
pim1Ran GDP/GTP exchange factor Affinity Capture-WesternPMID:8896453
Co-fractionation
rrp45exosome subunit Rrp45 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
ned1lipin Ned1 Two-hybridPMID:12376568
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
wee1M phase inhibitor protein kinase Wee1 Dosage RescuePMID:1944266
spf30splicing factor Spf30 (predicted) Phenotypic SuppressionPMID:20028739
pab2poly(A) binding protein Pab2 Phenotypic EnhancementPMID:21981922
Synthetic Growth DefectPMID:20129053
Phenotypic EnhancementPMID:20622014
dis1microtubule-associated protein Dis1 Synthetic LethalityPMID:1944266
swi6HP1 family chromodomain protein Swi6 Synthetic LethalityPMID:17380189
ppe1serine/threonine protein phosphatase Ppe1 Dosage RescuePMID:8387356
dcr1dicer Synthetic LethalityPMID:17380189
Phenotypic EnhancementPMID:20178743
ran1serine/threonine protein kinase Ran1 Phenotypic SuppressionPMID:16478992
mad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:17380189
Phenotypic Enhancement
dis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic LethalityPMID:1944266
ned1lipin Ned1 Synthetic Growth DefectPMID:12376568
cdc25M phase inducer tyrosine phosphatase Cdc25 Dosage RescuePMID:1944266
External References
Database Identifier Description
NBRP SPBC26H8.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC26H8.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC26H8.10 BioGRID Interaction Datasets
Expression Viewer SPBC26H8.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC26H8.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC26H8.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC26H8.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC26H8.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC26H8.10 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC26H8.10 Cell Cycle Data
GEO SPBC26H8.10 GEO profiles
PInt SPBC26H8.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC26H8.10 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC26H8.10 Fission yeast phenotypic data & analysis
SPD / RIKEN34/34A05Orfeome Localization Data
UniProtKB/SwissProtP37202Exosome complex exonuclease dis3
ModBaseP37202Database of comparative protein structure models
STRINGP37202Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_5966533'-5' exoribonuclease subunit Dis3 (predicted)
RefSeq mRNANM_001022575972h- 3'-5' exoribonuclease subunit Dis3 (predicted) (dis3), mRNA
European Nucleotide ArchiveAAA35302.1ENA Protein Mapping
European Nucleotide ArchiveCAA21102.1ENA Protein Mapping
UniParcUPI00001293F8UniProt Archive

Literature for dis3

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014