dis3 (SPBC26H8.10)


Gene Standard Namedis3 Characterisation Statuspublished
Systematic IDSPBC26H8.10 Feature Typeprotein coding
Synonymsrrp44 Name Descriptionchromosome DISjunction,
defective in sister chromatid disjoining
Product3'-5' exoribonuclease subunit Dis3 (predicted) Product Size970aa, 110.14 kDa
Genomic Location Chromosome II, 3970898-3974218 (3321nt); CDS:3970969-3974045 (3077nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:00001753'-5'-exoribonuclease activityISOSGD:S000005381GO_REF:000002416
GO:0003723RNA bindingIEAUniProtKB-KW:KW-0694GO_REF:0000037628
GO:0008536Ran GTPase bindingIPIpim1PMID:889645315
GO:0004521endoribonuclease activityISOSGD:S000005381GO_REF:000002435
GO:0005515protein bindingIPIned1PMID:12376568861
IPIspi1PMID:8896453
IPIpim1PMID:8896453
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0090502RNA phosphodiester bond hydrolysis, endonucleolyticISOSGD:S000005381GO_REF:000002423
GO:0000467exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)ISOSGD:S000005381GO_REF:000002415
GO:0000465exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)ISOSGD:S000005381GO_REF:00000241
GO:0000070mitotic sister chromatid segregationIMPPMID:3409871132
GO:0034661ncRNA catabolic processISOSGD:S000005381GO_REF:000002419
GO:0070651nonfunctional rRNA decayISOSGD:S000005381GO_REF:000002413
GO:0071042nuclear polyadenylation-dependent mRNA catabolic processISOSGD:S000005381GO_REF:000002412
GO:0071035nuclear polyadenylation-dependent rRNA catabolic processISOSGD:S000005381GO_REF:000002412
GO:0071038nuclear polyadenylation-dependent tRNA catabolic processISOSGD:S000005381GO_REF:000002412
GO:0070478nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decayISOSGD:S000005381GO_REF:000002413
GO:0070481nuclear-transcribed mRNA catabolic process, non-stop decayISOSGD:S000005381GO_REF:000002413
GO:0032853positive regulation of Ran GTPase activityIMPPMID:88964533
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000177cytoplasmic exosome (RNase complex)ISOSGD:S000005381GO_REF:000002410
GO:0000178exosome (RNase complex)IDAPMID:2350358814
GO:0000176nuclear exosome (RNase complex)ISOSGD:S000005381GO_REF:000002413
GO:0005634nucleusIDAPMID:168233722740
IDAPMID:1944266
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaydis3-54Not specifiedPECO:0000006, PECO:0000201, PECO:0000102PMID:3409871106
FYPO:0002061inviable vegetative cell populationMicroscopydis3ΔNullPECO:0000005, PECO:0000137PMID:236978061331
Microscopydis3ΔNullPMID:20473289
FYPO:0001164normal growth on glucose carbon sourceCell growth assaydis3-54Not specifiedPECO:0000102, PECO:0000004PMID:12376568156
FYPO:0000097sensitive to caffeineCell growth assaydis3-54Not specifiedPECO:0000201, PECO:0000227, PECO:0000004PMID:340987198
FYPO:0000080sensitive to coldCell growth assaydis3-54Not specifiedPECO:0000102PMID:1237656842
FYPO:0000091sensitive to thiabendazoleCell growth assaydis3-54Not specifiedPMID:3409871168

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopydis3ΔNullPECO:0000005, PECO:0000137PMID:23697806638
FYPO:0000671abnormal rDNA separationMicroscopydis3-54Not specifiedPECO:0000006PMID:34098713
FYPO:0001840increased minichromosome loss during vegetative growthMicroscopydis3-54Not specifiedPECO:0000004, PECO:0000227PMID:340987130
FYPO:0000314inviable after spore germination with elongated germ tubeMicroscopydis3ΔNullPECO:0000005, PECO:0000137PMID:23697806163
FYPO:0001042inviable after spore germination, single or double cell divisionMicroscopydis3ΔNullPECO:0000005, PECO:0000137PMID:2369780695
FYPO:0000049inviable cellEnzyme assay datadis3::LEU2NullPMID:19442661385
FYPO:0001761normal mitotic G1/S phase transitionFlow cytometry datadis3-54Not specifiedPECO:0000006PMID:19442663
FYPO:0001759normal protein phosphatase activityEnzyme assay datadis3-54Not specifiedPECO:0000006PMID:19442664
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in ned1 lipin Ned1 PMID:12376568
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
139708983971161
239713263974218

UTRs

Region Coordinates Reference
five_prime_UTR3970898..3970968PMID:21511999
three_prime_UTR3974046..3974218PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13638 Pfam IPR002716 PIN domain 86 209 2
PF00773 Pfam 496 828 5
SM00955 SMART 496 829 5
SM00670 SMART IPR002716 PIN domain 83 198 5
PS01175 Prosite Patterns IPR022966 Ribonuclease II/R, conserved site 796 820 2
PS50126 Prosite Profiles IPR003029 Ribosomal protein S1, RNA-binding domain 869 969 6
PTHR23355 HMMPANTHER 19 969 5
PTHR23355:SF35 HMMPANTHER 19 969 1
3.40.50.1010 Gene3D IPR029060 PIN domain-like 83 216 9
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 878 962 57
SSF88723 SuperFamily IPR029060 PIN domain-like 83 217 10
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 245 336 57
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 393 441 57
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 450 873 57

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001933PINc domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001933

Protein Properties

Ave. residue weight 113.54 Da
Charge 5.50
Isoelectric point 6.88
Molecular weight 110.14 kDa
Number of residues 970
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
7301during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
6084during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
6637during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
7097during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
6679during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
6514.75during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1625.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
5.2during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
oligomeric PMID:172082574
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1802510579
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
wee1M phase inhibitor protein kinase Wee1 Dosage RescuePMID:1944266
spf30splicing factor Spf30 (predicted) Phenotypic SuppressionPMID:20028739
pab2poly(A) binding protein Pab2 Phenotypic EnhancementPMID:21981922
Synthetic Growth DefectPMID:20129053
Phenotypic EnhancementPMID:20622014
dis1microtubule-associated protein Dis1 Synthetic LethalityPMID:1944266
swi6HP1 family chromodomain protein Swi6 Synthetic LethalityPMID:17380189
ppe1serine/threonine protein phosphatase Ppe1 Dosage RescuePMID:8387356
dcr1dicer Synthetic LethalityPMID:17380189
Phenotypic EnhancementPMID:20178743
ran1serine/threonine protein kinase Ran1 Phenotypic SuppressionPMID:16478992
mad2spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:17380189
Phenotypic Enhancement
dis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic LethalityPMID:1944266
ned1lipin Ned1 Synthetic Growth DefectPMID:12376568
cdc25M phase inducer tyrosine phosphatase Cdc25 Dosage RescuePMID:1944266
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rrp6exosome 3'-5' exoribonuclease subunit Rrp6 (predicted) Affinity Capture-MSPMID:23503588
dis33'-5' exoribonuclease subunit Dis3 (predicted) Affinity Capture-MSPMID:23503588
rrp43exosome subunit Rrp43 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
rrp4exosome subunit Rrp4 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
spi1Ran GTPase Spi1 Co-fractionationPMID:8896453
Affinity Capture-Western
rrp40exosome subunit Rrp40 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
pim1Ran GDP/GTP exchange factor Affinity Capture-WesternPMID:8896453
Co-fractionation
cti1Cut3 interacting protein Cti1, predicted exosome subunit Affinity Capture-MSPMID:23503588
ski7ski complex interacting GTPase Ski7 Affinity Capture-MSPMID:23503588
mtr3exosomal 3'-5' exoribonuclease, PH-like Mtr3 Affinity Capture-MSPMID:23503588
csl4exosome subunit Csl4 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
rrp46exosome subunit Rrp46 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
pab2poly(A) binding protein Pab2 Affinity Capture-WesternPMID:20129053
rrp41exosomal 3'-to-5' phosphorolytic exoribonuclease Rrp41 Affinity Capture-MSPMID:23503588
rrp42exosome subunit Rrp42 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
rrp45exosome subunit Rrp45 Affinity Capture-MSPMID:23755256
Affinity Capture-MSPMID:23503588
act1actin Act1 Affinity Capture-MSPMID:23755256
ned1lipin Ned1 Two-hybridPMID:12376568
External References
Database Identifier Description
NBRP SPBC26H8.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC26H8.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC26H8.10 BioGRID Interaction Datasets
Expression Viewer SPBC26H8.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC26H8.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC26H8.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC26H8.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC26H8.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC26H8.10 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC26H8.10 Cell Cycle Data
GEO SPBC26H8.10 GEO profiles
PInt SPBC26H8.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC26H8.10 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN34/34A05Orfeome Localization Data
WikiGene25403983'-5' exoribonuclease subunit Dis3 (predicted)
EntrezGene25403983'-5' exoribonuclease subunit Dis3 (predicted)
UniProtKB/SwissProtP37202Exosome complex exonuclease dis3
ModBaseP37202Database of comparative protein structure models
STRINGP37202Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_5966533'-5' exoribonuclease subunit Dis3 (predicted)
RefSeq mRNANM_001022575972h- 3'-5' exoribonuclease subunit Dis3 (predicted) (dis3), mRNA
European Nucleotide ArchiveAAA35302ENA Protein Mapping
European Nucleotide ArchiveAAA35302.1ENA Protein Mapping
European Nucleotide ArchiveCAA21102ENA Protein Mapping
European Nucleotide ArchiveCAA21102.1ENA Protein Mapping
UniParcUPI00001293F8UniProt Archive

Literature for dis3

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014