dis3 (SPBC26H8.10)

Gene Standard Namedis3 Characterisation Statuspublished
Systematic IDSPBC26H8.10 Feature Typeprotein coding
Synonymsrrp44 Name Descriptionchromosome DISjunction,
defective in sister chromatid disjoining
Product3'-5' exoribonuclease subunit Dis3 Product Size970aa, 110.14 kDa
Genomic Location Chromosome II, 3970898-3974218 (3321nt); CDS:3970969-3974045 (3077nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
3'-5'-exoribonuclease activity18
Annotation ExtensionEvidenceWith/FromReference
endoribonuclease activity36
Annotation ExtensionEvidenceWith/FromReference
protein binding900
Annotation ExtensionEvidenceWith/FromReference
Ran GTPase binding14
Annotation ExtensionEvidenceWith/FromReference
ribonuclease activity59
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)15
Annotation ExtensionEvidenceWith/FromReference
exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)1
Annotation ExtensionEvidenceWith/FromReference
ncRNA catabolic process21
Annotation ExtensionEvidenceWith/FromReference
nonfunctional rRNA decay13
Annotation ExtensionEvidenceWith/FromReference
nuclear polyadenylation-dependent mRNA catabolic process12
Annotation ExtensionEvidenceWith/FromReference
nuclear polyadenylation-dependent rRNA catabolic process14
Annotation ExtensionEvidenceWith/FromReference
nuclear polyadenylation-dependent tRNA catabolic process12
Annotation ExtensionEvidenceWith/FromReference
nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay17
Annotation ExtensionEvidenceWith/FromReference
nuclear-transcribed mRNA catabolic process, meiosis-specific transcripts10
Annotation ExtensionEvidenceWith/FromReference
nuclear-transcribed mRNA catabolic process, non-stop decay15
Annotation ExtensionEvidenceWith/FromReference
positive regulation of GTPase activity65
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasmic exosome (RNase complex)10
Annotation ExtensionEvidenceWith/FromReference
exosome (RNase complex)14
Annotation ExtensionEvidenceWith/FromReference
Mmi1 nuclear focus complex8
Annotation ExtensionEvidenceWith/FromReference
nuclear exosome (RNase complex)13
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at low temperaturedis3-54 (P509L)96
decreased cell population growth on glucose carbon sourcedis3-54 (P509L)393
decreased vegetative cell population growthdis3-54 (P509L)745
inviable cell populationdis3::LEU21482
inviable vegetative cell populationdis3Δ1468
dis3-54 (P509L)
normal growth on glucose carbon sourcedis3-54 (P509L)318
sensitive to caffeine during vegetative growthdis3-54 (P509L)110
sensitive to thiabendazoledis3-54 (P509L)334
viable vegetative cell populationdis3-54 (P509L)3862

Cell Phenotype

Term NameGenotypesCount
abnormal attachment of spindle microtubules to kinetochore involved in homologous chromosome segregationdis3-54 (P509L)6
abnormal cell cycle arrest in mitotic metaphasedis3-54 (P509L)20
abnormal mitotic cell cycledis3Δ903
abnormal rDNA separationdis3-54 (P509L)6
abolished mitotic sister chromatid separationdis3-54 (P509L)39
decreased chromatin silencing at centromere inner repeatdis3-54 (P509L)12
decreased chromatin silencing at centromere outer repeatdis3-54 (P509L)51
decreased chromatin silencing at silent mating-type cassettedis3-54 (P509L)30
decreased ribonuclease activitydis3-54 (P509L)1
dis3-RNB (T808A)
decreased transcriptional repression at centromere central coredis3-54 (P509L)9
increased activation of mitotic cell cycle spindle assembly checkpointdis3-54 (P509L)16
increased centromeric outer repeat transcript leveldis3-54 (P509L)33
increased level of DSR-containing meiosis gene mRNA during vegetative growthdis3-ts414
increased minichromosome loss during vegetative growthdis3-54 (P509L)81
increased polyadenylated 25S rRNA leveldis3-54 (P509L)1
increased pre-mRNA level89
affecting rps2202dis3-54 (P509L)
increased protein localization to kinetochore during vegetative growth7
affects localization of mad2dis3-54 (P509L)
affects localization of bub1dis3-54 (P509L)
increased RNA level during vegetative growth356
affecting mei2dis3-54 (P509L)
affecting ste11dis3-54 (P509L)
inviable after spore germination with elongated germ tubedis3Δ164
inviable after spore germination, single or double cell divisiondis3Δ107
inviable sporedis3::LEU2476
long mitotic spindledis3-54 (P509L)24
mitotic spindle collapse without elongationdis3-54 (P509L)9
normal centromeric outer repeat transcript-derived siRNA leveldis3-54 (P509L)9
normal mitotic G1/S phase transitiondis3-54 (P509L)3
normal polyadenylated 5.8S rRNA leveldis3-54 (P509L)1
normal protein phosphatase activitydis3-54 (P509L)4

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable cell populationdis3-54 (P509L), dis2-11
inviable vegetative cell populationdis3+, cdc25-22 (C532Y), wee1-50
dis3-54 (P509L), dis3-172E (1-547)
dis3-54 (P509L), dis3-172C (1-398)
dis3-54 (P509L), dis3-172D (1-445)
dis3-54 (P509L), sds21Δ
dis3-172G (670-970), dis3-54 (P509L)
dis3-172H (442-970), dis3-54 (P509L)
dis3-54 (P509L), dis2Δ
dis1-288, dis3-54 (P509L)
dis3-54 (P509L), pab2Δ
dis3-54 (P509L), dis3-172B (1-225)
dis3-54 (P509L), dis3-172F (824-970)
normal growth on okadaic aciddis2-11, dis3-54 (P509L), sds21Δ, ppe1Δ, dis1-288, dis2Δ, ppa1Δ
slow vegetative cell population growthdis3-172A (174), dis3-54 (P509L)
viable vegetative cell populationdis3-54 (P509L), dis3-172C (1-398)
dis3-172H (442-970), dis3-54 (P509L)
dis3-172A (174), dis3-54 (P509L)
dis3-54 (P509L), dis3-172B (1-225)
dis3-54 (P509L), dis3-172F (824-970)
dis3-172G (670-970), dis3-54 (P509L)
dis3+, cdc25-22 (C532Y), wee1-50
dis3-54 (P509L), dis3-172D (1-445)
dis3-54 (P509L), dis3-172E (1-547)
dis3-54 (P509L), sds21Δ

Cell Phenotype

Term NameGenotypes
increased snoRNA level
affecting sno12dis3-54 (P509L), pab2Δ
inviable after spore germination, multiple cell divisionsdis1-288, dis3-54 (P509L)
inviable after spore germination, without cell divisiondis3-54 (P509L), dis2Δ
inviable sporedis3-54 (P509L), dis2-11
Target Of
FYPO affected by mutation in mtl1 TRAMP/MTREC complex subunit, ATP-dependent RNA helicase (predicted)
FYPO affected by mutation in ned1 lipin, phosphatidate phosphatase Ned1
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3970898..3971161, 3971326..3974218
5' UTR3970898..3970968PMID:21511999
CDS3970969..3971161, 3971326..3974045
3' UTR3974046..3974218PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF17215 Pfam IPR033770 Exosome complex exonuclease RRP44, S1 domain 878 965 1
PF13638 Pfam IPR002716 PIN domain 86 209 2
PF00773 Pfam 496 827 5
PF17216 Pfam IPR033771 Rrp44-like cold shock domain 251 334 1
SM00670 SMART IPR002716 PIN domain 83 198 5
SM00955 SMART 496 829 5
PS01175 Prosite Patterns IPR022966 Ribonuclease II/R, conserved site 796 820 2
PS50126 Prosite Profiles IPR003029 S1 domain 869 969 5
PTHR23355 HMMPANTHER 11 969 5
PTHR23355:SF35 HMMPANTHER 11 969 1 Gene3D IPR029060 PIN domain-like 83 216 9
SSF88723 SuperFamily IPR029060 PIN domain-like 83 217 10
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 878 962 57
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 450 873 57
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 245 336 57
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 393 441 57

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001933PINc domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001933

Protein Properties

Ave. residue weight 113.54 Da
Charge 5.50
Codon Adaptation Index 0.41
Isoelectric point 6.88
Molecular weight 110.14 kDa
Number of residues 970
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for dis3 (SPBC26H8.10)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7301during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
6084during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
6637during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
7097during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
6514.75during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
6679during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
1625.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.2during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Subunit Composition
oligomeric PMID:172082574
deletion mutant expression profiling PMID:1802510579
Taxonomic Conservation
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in bacteria1005
conserved in metazoa3498
conserved in vertebrates3473
conserved in archaea243

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC26H8.10 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesrrp46exosome subunit Rrp46 Affinity Capture-MSPMID:23503588
affinity capturesrrp6exosome 3'-5' exoribonuclease subunit Rrp6 (predicted) Affinity Capture-MSPMID:23503588
affinity capturesrrp41exosomal 3'-to-5' phosphorolytic exoribonuclease Rrp41 Affinity Capture-MSPMID:23503588
affinity capturespab2poly(A) binding protein Pab2 Affinity Capture-WesternPMID:20129053
affinity capturesrrp42exosome subunit Rrp42 Affinity Capture-MSPMID:23503588
affinity capturesrrp45exosome subunit Rrp45 Affinity Capture-MSPMID:23755256
affinity capturesdis33'-5' exoribonuclease subunit Dis3 Affinity Capture-MSPMID:23503588
affinity capturescti1C1D family exosome subunit Cti1 Affinity Capture-MSPMID:23503588
affinity capturescsl4exosome subunit Csl4 Affinity Capture-MSPMID:23503588
affinity capturesrrp4exosome subunit Rrp4 Affinity Capture-MSPMID:23503588
affinity capturesrrp40exosome subunit Rrp40 Affinity Capture-MSPMID:23755256
affinity capturesact1actin Act1 Affinity Capture-MSPMID:23755256
affinity capturesrrp43exosome subunit Rrp43 Affinity Capture-MSPMID:23755256
affinity capturesski7ski complex interacting GTPase Ski7 Affinity Capture-MSPMID:23503588
affinity capturesmtr3exosomal 3'-5' exoribonuclease, PH-like Mtr3 Affinity Capture-MSPMID:23503588
affinity captured bypim1RCC1 family Ran GDP/GTP exchange factor Affinity Capture-WesternPMID:8896453
affinity captured byiss10Mmi1-driven selective elimination factor Iss10 Affinity Capture-MSPMID:24210919
affinity captured byspi1Ran GTPase Spi1 Affinity Capture-WesternPMID:8896453
affinity captured byrrp43exosome subunit Rrp43 Affinity Capture-MSPMID:23503588
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24210919
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
binds DNA-binding domain construct withned1lipin, phosphatidate phosphatase Ned1 Two-hybridPMID:12376568
co-fractionates withpim1RCC1 family Ran GDP/GTP exchange factor Co-fractionationPMID:8896453
co-fractionates withspi1Ran GTPase Spi1 Co-fractionationPMID:8896453
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC26H8.10 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withdis2serine/threonine protein phosphatase PP1, Dis2 Synthetic LethalityPMID:1944266
synthetic lethal withswi6HP1 family chromodomain protein Swi6 Synthetic LethalityPMID:17380189
synthetic lethal withdcr1dicer Synthetic LethalityPMID:17380189
synthetic lethal withdis1TOG/XMAP14 microtubule-associated protein Dis1 Synthetic LethalityPMID:1944266
synthetic growth defect withned1lipin, phosphatidate phosphatase Ned1 Synthetic Growth DefectPMID:12376568
synthetic growth defect withmad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:17380189
synthetic growth defect withpab2poly(A) binding protein Pab2 Synthetic Growth DefectPMID:20129053
enhances phenotype ofpab2poly(A) binding protein Pab2 Phenotypic EnhancementPMID:21981922
enhances phenotype ofdcr1dicer Phenotypic EnhancementPMID:20178743
rescuesspf30splicing factor Spf30 (predicted) Phenotypic SuppressionPMID:20028739
rescuespat1serine/threonine protein kinase Ran1/Pat1 Phenotypic SuppressionPMID:16478992
overexpression rescuescdc25M phase inducer tyrosine phosphatase Cdc25 Dosage RescuePMID:1944266
overexpression rescuesppe1serine/threonine protein phosphatase Ppe1 Dosage RescuePMID:8387356
overexpression rescueswee1M phase inhibitor protein kinase Wee1 Dosage RescuePMID:1944266
phenotype enhanced bymad2mitotic spindle checkpoint protein Mad2 Phenotypic EnhancementPMID:17380189
External References
Database Identifier Description
NBRP SPBC26H8.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC26H8.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC26H8.10 BioGRID Interaction Datasets
Expression Viewer SPBC26H8.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC26H8.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC26H8.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC26H8.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC26H8.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC26H8.10 Transcriptome Viewer (Bähler Lab)
GEO SPBC26H8.10 GEO profiles
PInt SPBC26H8.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC26H8.10 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC26H8.10 Fission yeast phenotypic data & analysis
Cyclebase SPBC26H8.10.1 Cell Cycle Data
SPD / RIKEN34/34A05Orfeome Localization Data
UniProtKB/SwissProtP37202Exosome complex exonuclease dis3
ModBaseP37202Database of comparative protein structure models
STRINGP37202Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_5966533'-5' exoribonuclease subunit Dis3 (predicted)
RefSeq mRNANM_001022575972h- 3'-5' exoribonuclease subunit Dis3 (predicted) (dis3), mRNA
European Nucleotide ArchiveAAA35302.1ENA Protein Mapping
European Nucleotide ArchiveCAA21102.1ENA Protein Mapping
UniParcUPI00001293F8UniProt Archive

Literature for dis3

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017