dis3 (SPBC26H8.10)


Gene Standard Namedis3 Characterisation Statuspublished
Systematic IDSPBC26H8.10 Feature Typeprotein coding
Synonymsrrp44 Name Descriptionchromosome DISjunction,
defective in sister chromatid disjoining
Product3'-5' exoribonuclease subunit Dis3 Product Size970aa, 110.14 kDa
Genomic Location Chromosome II, 3970898-3974218 (3321nt); CDS:3970969-3974045 (3077nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
3'-5'-exoribonuclease activity16
Annotation ExtensionEvidenceWith/FromReference
endoribonuclease activity36
Annotation ExtensionEvidenceWith/FromReference
protein binding859
Annotation ExtensionEvidenceWith/FromReference
Ran GTPase binding15
Annotation ExtensionEvidenceWith/FromReference
ribonuclease activity57
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)15
Annotation ExtensionEvidenceWith/FromReference
exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)1
Annotation ExtensionEvidenceWith/FromReference
ncRNA catabolic process22
Annotation ExtensionEvidenceWith/FromReference
nonfunctional rRNA decay13
Annotation ExtensionEvidenceWith/FromReference
nuclear polyadenylation-dependent mRNA catabolic process12
Annotation ExtensionEvidenceWith/FromReference
nuclear polyadenylation-dependent rRNA catabolic process13
Annotation ExtensionEvidenceWith/FromReference
nuclear polyadenylation-dependent tRNA catabolic process12
Annotation ExtensionEvidenceWith/FromReference
nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay17
Annotation ExtensionEvidenceWith/FromReference
nuclear-transcribed mRNA catabolic process, meiosis-specific transcripts9
Annotation ExtensionEvidenceWith/FromReference
nuclear-transcribed mRNA catabolic process, non-stop decay15
Annotation ExtensionEvidenceWith/FromReference
positive regulation of GTPase activity69
Annotation ExtensionEvidenceWith/FromReference
RNA phosphodiester bond hydrolysis53
Annotation ExtensionEvidenceWith/FromReference
RNA phosphodiester bond hydrolysis, endonucleolytic26
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasmic exosome (RNase complex)10
Annotation ExtensionEvidenceWith/FromReference
exosome (RNase complex)14
Annotation ExtensionEvidenceWith/FromReference
Mmi1 nuclear focus complex7
Annotation ExtensionEvidenceWith/FromReference
nuclear exosome (RNase complex)13
Annotation ExtensionEvidenceWith/FromReference
nucleolus363
Annotation ExtensionEvidenceWith/FromReference
nucleoplasm248
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at low temperaturedis3-54 (P509L)Not specified61
decreased cell population growth on glucose carbon sourcedis3-54 (P509L)Not specified258
decreased vegetative cell population growthdis3-54 (P509L)Not specified571
inviable vegetative cell populationdis3-54 (P509L)Not specified1438
dis3::LEU2Null
dis3ΔNull
normal growth on glucose carbon sourcedis3-54 (P509L)Not specified207
sensitive to caffeine during vegetative growthdis3-54 (P509L)Not specified106
sensitive to thiabendazoledis3-54 (P509L)Not specified219

Cell Phenotype

Term NameAlleleExpressionCount
abnormal attachment of spindle microtubules to kinetochore involved in homologous chromosome segregationdis3-54 (P509L)Not specified4
abnormal cell cycle arrest at mitotic metaphase/anaphase transitiondis3-54 (P509L)Not specified6
abnormal mitotic cell cycledis3ΔNull834
abnormal rDNA separationdis3-54 (P509L)Not specified4
abolished mitotic sister chromatid separationdis3-54 (P509L)Not specified26
decreased chromatin silencing at centromere central coredis3-54 (P509L)Endogenous9
decreased chromatin silencing at centromere inner repeatdis3-54 (P509L)Endogenous8
decreased chromatin silencing at centromere outer repeat regiondis3-54 (P509L)Not specified35
decreased chromatin silencing at silent mating-type cassettedis3-54 (P509L)Endogenous19
decreased ribonuclease activitydis3-54 (P509L)Endogenous1
dis3-RNB (T808A)Endogenous
increased activation of mitotic cell cycle spindle assembly checkpointdis3-54 (P509L)Not specified14
increased centromeric outer repeat transcript leveldis3-54 (P509L)Not specified19
increased level of DSR-containing meiosis gene RNA during vegetative growthdis3-ts4Not specified14
increased minichromosome loss during vegetative growthdis3-54 (P509L)Not specified46
increased protein localization to kinetochore during vegetative growth4
affecting mad2dis3-54 (P509L)Not specified
affecting bub1dis3-54 (P509L)Not specified
inviable after spore germination with elongated germ tubedis3ΔNull164
inviable after spore germination, single or double cell divisiondis3ΔNull103
long mitotic spindledis3-54 (P509L)Not specified14
mitotic spindle collapsedis3-54 (P509L)Not specified7
normal centromeric outer repeat transcript-derived siRNA leveldis3-54 (P509L)Endogenous8
normal mitotic G1/S phase transitiondis3-54 (P509L)Not specified3
normal protein phosphatase activitydis3-54 (P509L)Not specified4
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in mtl1 TRAMP complex ATP-dependent RNA helicase (predicted)
FYPO affected by mutation in ned1 lipin, phosphatidate phosphatase Ned1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3970898..3971161, 3971326..3974218
mRNA3970898..3974218
5' UTR3970898..3970968PMID:21511999
CDS3970969..3971161, 3971326..3974045
3' UTR3974046..3974218PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00773 Pfam 496 828 5
PF13638 Pfam IPR002716 PIN domain 86 209 2
SM00955 SMART 496 829 5
SM00670 SMART IPR002716 PIN domain 83 198 5
PS50126 Prosite Profiles IPR003029 Ribosomal protein S1, RNA-binding domain 869 969 6
PS01175 Prosite Patterns IPR022966 Ribonuclease II/R, conserved site 796 820 2
PTHR23355:SF35 HMMPANTHER 19 969 1
PTHR23355 HMMPANTHER 19 969 5
3.40.50.1010 Gene3D IPR029060 PIN domain-like 83 216 9
SSF88723 SuperFamily IPR029060 PIN domain-like 83 217 10
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 878 962 57
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 450 873 57
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 245 336 57
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 393 441 57

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001933PINc domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001933

Protein Properties

Ave. residue weight 113.54 Da
Charge 5.50
Isoelectric point 6.88
Molecular weight 110.14 kDa
Number of residues 970
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7301during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6084during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6637during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7097during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6679during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6514.75during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1625.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
oligomeric PMID:172082574
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1802510577
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
conserved in archaea238
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesact1actin Act1 Affinity Capture-MSPMID:23755256
affinity capturescsl4exosome subunit Csl4 Affinity Capture-MSPMID:23755256
affinity capturescsl4exosome subunit Csl4 Affinity Capture-MSPMID:23503588
affinity capturescti1Cut3 interacting protein Cti1, predicted exosome subunit Affinity Capture-MSPMID:23503588
affinity capturesdis33'-5' exoribonuclease subunit Dis3 Affinity Capture-MSPMID:23503588
affinity captured byiss10Mmi1-driven selective elimination factor Iss10 Affinity Capture-MSPMID:24713849
affinity captured byiss10Mmi1-driven selective elimination factor Iss10 Affinity Capture-MSPMID:24210919
affinity capturesmtr3exosomal 3'-5' exoribonuclease, PH-like Mtr3 Affinity Capture-MSPMID:23503588
binds DNA-binding domain construct withned1lipin, phosphatidate phosphatase Ned1 Two-hybridPMID:12376568
affinity capturespab2poly(A) binding protein Pab2 Affinity Capture-WesternPMID:20129053
affinity captured bypim1RCC1 family Ran GDP/GTP exchange factor Affinity Capture-WesternPMID:8896453
co-fractionates withpim1RCC1 family Ran GDP/GTP exchange factor Co-fractionationPMID:8896453
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24210919
affinity capturesrrp4exosome subunit Rrp4 Affinity Capture-MSPMID:23755256
affinity capturesrrp4exosome subunit Rrp4 Affinity Capture-MSPMID:23503588
affinity capturesrrp40exosome subunit Rrp40 Affinity Capture-MSPMID:23755256
affinity capturesrrp40exosome subunit Rrp40 Affinity Capture-MSPMID:23503588
affinity capturesrrp41exosomal 3'-to-5' phosphorolytic exoribonuclease Rrp41 Affinity Capture-MSPMID:23503588
affinity capturesrrp42exosome subunit Rrp42 Affinity Capture-MSPMID:23755256
affinity capturesrrp42exosome subunit Rrp42 Affinity Capture-MSPMID:23503588
affinity capturesrrp43exosome subunit Rrp43 Affinity Capture-MSPMID:23755256
affinity capturesrrp43exosome subunit Rrp43 Affinity Capture-MSPMID:23503588
affinity captured byrrp43exosome subunit Rrp43 Affinity Capture-MSPMID:23503588
affinity capturesrrp45exosome subunit Rrp45 Affinity Capture-MSPMID:23755256
affinity capturesrrp45exosome subunit Rrp45 Affinity Capture-MSPMID:23503588
affinity capturesrrp46exosome subunit Rrp46 Affinity Capture-MSPMID:23755256
affinity capturesrrp46exosome subunit Rrp46 Affinity Capture-MSPMID:23503588
affinity capturesrrp6exosome 3'-5' exoribonuclease subunit Rrp6 (predicted) Affinity Capture-MSPMID:23503588
affinity capturesski7ski complex interacting GTPase Ski7 Affinity Capture-MSPMID:23503588
affinity captured byspi1Ran GTPase Spi1 Affinity Capture-WesternPMID:8896453
co-fractionates withspi1Ran GTPase Spi1 Co-fractionationPMID:8896453
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuescdc25M phase inducer tyrosine phosphatase Cdc25 Dosage RescuePMID:1944266
synthetic lethal withdcr1dicer Synthetic LethalityPMID:17380189
enhances phenotype ofdcr1dicer Phenotypic EnhancementPMID:20178743
synthetic lethal withdis1TOG/XMAP14 microtubule-associated protein Dis1 Synthetic LethalityPMID:1944266
synthetic lethal withdis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic LethalityPMID:1944266
phenotype enhanced bymad2mitotic spindle checkpoint protein Mad2 Phenotypic EnhancementPMID:17380189
synthetic growth defect withmad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:17380189
synthetic growth defect withned1lipin, phosphatidate phosphatase Ned1 Synthetic Growth DefectPMID:12376568
enhances phenotype ofpab2poly(A) binding protein Pab2 Phenotypic EnhancementPMID:21981922
synthetic growth defect withpab2poly(A) binding protein Pab2 Synthetic Growth DefectPMID:20129053
enhances phenotype ofpab2poly(A) binding protein Pab2 Phenotypic EnhancementPMID:20622014
rescuespat1serine/threonine protein kinase Ran1/Pat1 Phenotypic SuppressionPMID:16478992
overexpression rescuesppe1serine/threonine protein phosphatase Ppe1 Dosage RescuePMID:8387356
rescuesspf30splicing factor Spf30 (predicted) Phenotypic SuppressionPMID:20028739
synthetic lethal withswi6HP1 family chromodomain protein Swi6 Synthetic LethalityPMID:17380189
overexpression rescueswee1M phase inhibitor protein kinase Wee1 Dosage RescuePMID:1944266
External References
Database Identifier Description
NBRP SPBC26H8.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC26H8.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC26H8.10 BioGRID Interaction Datasets
Expression Viewer SPBC26H8.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC26H8.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC26H8.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC26H8.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC26H8.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC26H8.10 Transcriptome Viewer (Bähler Lab)
GEO SPBC26H8.10 GEO profiles
PInt SPBC26H8.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC26H8.10 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC26H8.10 Fission yeast phenotypic data & analysis
Cyclebase SPBC26H8.10.1 Cell Cycle Data
SPD / RIKEN34/34A05Orfeome Localization Data
UniProtKB/SwissProtP37202Exosome complex exonuclease dis3
ModBaseP37202Database of comparative protein structure models
STRINGP37202Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_5966533'-5' exoribonuclease subunit Dis3 (predicted)
RefSeq mRNANM_001022575972h- 3'-5' exoribonuclease subunit Dis3 (predicted) (dis3), mRNA
European Nucleotide ArchiveAAA35302.1ENA Protein Mapping
European Nucleotide ArchiveCAA21102.1ENA Protein Mapping
UniParcUPI00001293F8UniProt Archive

Literature for dis3

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015