sua1 (SPBC27.08c)

Gene Standard Namesua1 Characterisation Statuspublished
Systematic IDSPBC27.08c Feature Typeprotein coding
Synonymsasp1, met3, SPBC28F2.01c Name Description
Productsulfate adenylyltransferase Product Size490aa, 54.75 kDa
Genomic Location Chromosome II, 1573100-1571423 (1678nt); CDS:1572957-1571485 (1473nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
sulfate adenylyltransferase (ATP) activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cysteine biosynthetic process11
Annotation ExtensionEvidenceWith/FromReference
hydrogen sulfide biosynthetic process9
Annotation ExtensionEvidenceWith/FromReference
methionine biosynthetic process30
Annotation ExtensionEvidenceWith/FromReference
sulfate assimilation8
Annotation ExtensionEvidenceWith/FromReference
sulfate assimilation via adenylyl sulfate reduction1
Annotation ExtensionEvidenceWith/FromReference
sulfur amino acid metabolic process40
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
abolished cell population growth on glycerol carbon sourcesua1ΔNull65
abolished cell population growth on methionine sulfur sourceSeR-2Not specified1
abolished cell population growth on sulfate sulfur sourceSeR-2Not specified1
decreased vegetative cell population growthsua1ΔNull400
growth auxotrophic for cysteinesua1 unnamed mutantNot specified10
growth auxotrophic for methioninesua1 unnamed mutantNot specified10
resistance to selenateSeR-2Not specified1
sensitive to cadmium246
expressivity FYPO_EXT:0000002sua1ΔNull
sensitive to hydrogen peroxidesua1ΔNull137
expressivity FYPO_EXT:0000003sua1ΔNull
viable vegetative cell populationsua1ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
decreased sulfate importSeR-2Not specified1
viable vacuolated vegetative cell, abnormal cell shape, normal cell sizesua1ΔNull14
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01747 Pfam IPR024951 Sulphate adenylyltransferase catalytic domain 167 385 1
PF14306 Pfam IPR025980 ATP-sulfurylase PUA-like domain 6 159 1
PTHR11055 HMMPANTHER 1 395 2
3.10.400.10 Gene3D 7 180 1 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 390 467 288 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 181 389 39
SSF52374 SuperFamily 167 386 24
SSF88697 SuperFamily IPR015947 PUA-like domain 6 166 8
MF_03106 hamap IPR027535 Sulfate adenylyltransferase 1 490 1
TIGR00339 tigrfam IPR002650 Sulphate adenylyltransferase 6 382 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.74 Da
Charge 3.50
Isoelectric point 7.14
Molecular weight 54.75 kDa
Number of residues 490

Protein Modifications

Term NameResidueCount
O-phospho-L-threonine 693
present during cellular response to thiabendazoleT356
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA T356 PMID:18257517
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
603058during GO:0000080PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
567856during GO:0000084PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
585821during GO:0000085PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
582513during GO:0000087PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
574335during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
140361.25during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
28115.01during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
78during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
4.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
functionally complements S. cerevisiae MET3full PMID:18388974
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3432
conserved in vertebrates3407

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
sua1sulfate adenylyltransferase Two-hybridPMID:23695164
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
sck2serine/threonine protein kinase Sck2 Phenotypic EnhancementPMID:24463365
Positive Genetic
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
iwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
mug81ATP-dependent RNA helicase Slh1, human ASCC3 ortholog (predicted) Positive GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
jmj2histone demethylase Jmj2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC27.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC27.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC27.08c BioGRID Interaction Datasets
Expression Viewer SPBC27.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC27.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC27.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC27.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC27.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC27.08c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC27.08c Cell Cycle Data
GEO SPBC27.08c GEO profiles
PInt SPBC27.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC27.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC27.08c Fission yeast phenotypic data & analysis
SPD / RIKEN23/23G03Orfeome Localization Data
IntEnz2.7.7.4Integrated relational Enzyme database
Rhea2.7.7.4Annotated reactions database
UniProtKB/SwissProtP78937Sulfate adenylyltransferase
ModBaseP78937Database of comparative protein structure models
STRINGP78937Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595662sulfate adenylyltransferase
RefSeq mRNANM_001021556972h- sulfate adenylyltransferase (sua1), mRNA
European Nucleotide ArchiveAAN32720.1ENA Protein Mapping
European Nucleotide ArchiveBAA12186.1ENA Protein Mapping
European Nucleotide ArchiveCAB89007.1ENA Protein Mapping
MetaCycPWY-5278Sulfite oxidation III
MetaCycPWY-5340Sulfate activation for sulfonation
MetaCycPWY-6683Sulfate reduction III (assimilatory)
MetaCycPWY-6932Selenate reduction
KEGG_Enzyme00230+ metabolism
KEGG_Enzyme00450+ metabolism
KEGG_Enzyme00920+ metabolism
UniParcUPI000012EF49UniProt Archive
UniPathwayUPA00140Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1

Literature for sua1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015