sua1 (SPBC27.08c)


Gene Standard Namesua1 Characterisation Statuspublished
Systematic IDSPBC27.08c Feature Typeprotein coding
Synonymsasp1, met3, SPBC28F2.01c Name Description
Productsulfate adenylyltransferase Product Size490aa, 54.75 kDa
Genomic Location Chromosome II, 1573100-1571423 (1678nt); CDS:1572957-1571485 (1473nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
sulfate adenylyltransferase (ATP) activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular amino acid biosynthetic process117
Annotation ExtensionEvidenceWith/FromReference
hydrogen sulfide biosynthetic process5
Annotation ExtensionEvidenceWith/FromReference
sulfate assimilation9
Annotation ExtensionEvidenceWith/FromReference
sulfate assimilation via adenylyl sulfate reduction1
Annotation ExtensionEvidenceWith/FromReference
sulfur amino acid metabolic process34
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4211
Annotation ExtensionEvidenceWith/FromReference
cytosol2307
Annotation ExtensionEvidenceWith/FromReference
mitochondrion752
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
abolished cell population growth on glycerol carbon sourcesua1Δ68
abolished cell population growth on methionine sulfur sourceSeR-21
abolished cell population growth on sulfate sulfur sourceSeR-22
decreased vegetative cell population growthsua1Δ614
growth auxotrophic for cysteinesua1 unnamed mutant9
growth auxotrophic for methioninesua1 unnamed mutant10
resistance to selenateSeR-21
sensitive to cadmiumsua1Δ249
sensitive to hydrogen peroxidesua1Δ141
viable vegetative cell populationsua1Δ3819

Cell Phenotype

Term NameGenotypesCount
decreased sulfate importSeR-21
viable vacuolated vegetative cell, abnormal cell shape, normal cell sizesua1Δ14
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1573100..1571423
mRNA1573100..1571423
5' UTR1573100..1572958PMID:21511999
CDS1572957..1571485
3' UTR1571484..1571423AU011039
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01747 Pfam IPR024951 Sulphate adenylyltransferase catalytic domain 169 383 1
PF14306 Pfam IPR025980 ATP-sulfurylase PUA-like domain 6 159 1
PTHR11055 HMMPANTHER 6 392 2
3.10.400.10 Gene3D PUA-like domain 7 180 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 390 467 288
3.40.50.620 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 181 389 39
SSF52374 SuperFamily 167 386 24
SSF88697 SuperFamily IPR015947 PUA-like domain 6 166 8
MF_03106 hamap IPR027535 Sulfate adenylyltransferase 1 490 1
TIGR00339 tigrfam IPR002650 Sulphate adenylyltransferase 6 382 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.74 Da
Charge 3.50
Isoelectric point 7.14
Molecular weight 54.75 kDa
Number of residues 490
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-threonine 699
present during cellular response to thiabendazoleT356
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA T356 PMID:18257517
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
603058during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
567856during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
585821during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
582513during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
140361.25during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
574335during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
28115.01during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
78during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
4.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae MET3full PMID:18388974
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3421
conserved in vertebrates3396
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPBC27.08c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withsua1sulfate adenylyltransferase Two-hybridPMID:23695164
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

Load gene that interact geneitically with SPBC27.08c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
negative genetic interaction withpuf1pumilio family RNA-binding protein Puf1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
positive genetic interaction withjmj2histone demethylase Jmj2 Positive GeneticPMID:22681890
positive genetic interaction withmug81ATP-dependent RNA helicase Slh1, human ASCC3 ortholog (predicted) Positive GeneticPMID:22681890
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withiwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
enhances phenotype ofsck2serine/threonine protein kinase Sck2 Phenotypic EnhancementPMID:24463365
External References
Database Identifier Description
NBRP SPBC27.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC27.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC27.08c BioGRID Interaction Datasets
Expression Viewer SPBC27.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC27.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC27.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC27.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC27.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC27.08c Transcriptome Viewer (Bähler Lab)
GEO SPBC27.08c GEO profiles
PInt SPBC27.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC27.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC27.08c Fission yeast phenotypic data & analysis
Cyclebase SPBC27.08c.1 Cell Cycle Data
IntEnz2.7.7.4Integrated relational Enzyme database
Rhea2.7.7.4Annotated reactions database
SPD / RIKEN23/23G03Orfeome Localization Data
UniProtKB/SwissProtP78937Sulfate adenylyltransferase
ModBaseP78937Database of comparative protein structure models
STRINGP78937Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595662sulfate adenylyltransferase
RefSeq mRNANM_001021556972h- sulfate adenylyltransferase (sua1), mRNA
European Nucleotide ArchiveAF421374ENA EMBL mapping
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveD83992ENA EMBL mapping
European Nucleotide ArchiveAAN32720ENA Protein Mapping
European Nucleotide ArchiveAAN32720.1ENA Protein Mapping
European Nucleotide ArchiveBAA12186ENA Protein Mapping
European Nucleotide ArchiveBAA12186.1ENA Protein Mapping
European Nucleotide ArchiveCAB89007ENA Protein Mapping
European Nucleotide ArchiveCAB89007.1ENA Protein Mapping
MetaCycPWY-5278Sulfite oxidation III
MetaCycPWY-5340Sulfate activation for sulfonation
MetaCycPWY-6683Sulfate reduction III (assimilatory)
MetaCycPWY-6932Selenate reduction
KEGG00230+2.7.7.4Purine metabolism
KEGG00261+2.7.7.4Monobactam biosynthesis
KEGG00450+2.7.7.4Selenocompound metabolism
KEGG00920+2.7.7.4Sulfur metabolism
UniParcUPI000012EF49UniProt Archive
UniPathwayUPA00140with

Literature for sua1

Search: Europe PMC or PubMed

Release Version: PomBase:29_56 - 12 Nov 2015