sua1 (SPBC27.08c)


Gene Standard Namesua1 Characterisation Statuspublished
Systematic IDSPBC27.08c Feature Typeprotein coding
Synonymsasp1, met3, SPBC28F2.01c Name Description
Productsulfate adenylyltransferase Product Size490aa, 54.75 kDa
Genomic Location Chromosome II, 1573100-1571423 (1678nt); CDS:1572957-1571485 (1473nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0004781sulfate adenylyltransferase (ATP) activityIMPPMID:183889741
IMPPMID:14723223
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0019344cysteine biosynthetic processIEAUniProtKB-KW:KW-0198GO_REF:000003711
GO:0070814hydrogen sulfide biosynthetic processIEA UniPathway:UPA00140GO_REF:00000419
GO:0009086methionine biosynthetic processIEAUniProtKB-KW:KW-0486GO_REF:000003730
GO:0000103sulfate assimilationIMPPMID:183889748
GO:0010134sulfate assimilation via adenylyl sulfate reductionIMPPMID:147232231
GO:0000096sulfur amino acid metabolic processIMPPMID:1762253340
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISSUniProtKB:P08536GO_REF:00000244205
GO:0005829cytosolIDAPMID:168233722315
GO:0005739mitochondrionISSUniProtKB:P08536GO_REF:0000024758
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001934abolished cell population growth on glycerol carbon sourceCell growth assaysua1ΔNullPECO:0000072PMID:1866526861
FYPO:0002752abolished cell population growth on methionine sulfur sourceCell growth assaySeR-2Not specifiedPMID:147232231
FYPO:0002753abolished cell population growth on sulfate sulfur sourceCell growth assaySeR-2Not specifiedPMID:147232231
FYPO:0001355decreased vegetative cell population growthCell growth assaysua1ΔNullPECO:0000126PMID:18665268381
FYPO:0000037growth auxotrophic for cysteineCell growth assaysua1 unnamed mutantNot specifiedPECO:0000126, PECO:0000005, PECO:0000102PMID:176225339
FYPO:0000040growth auxotrophic for methionineCell growth assaysua1 unnamed mutantNot specifiedPECO:0000126, PECO:0000005, PECO:0000102PMID:1762253310
FYPO:0000752resistance to selenateCell growth assaySeR-2Not specifiedPMID:147232231
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000002Cell growth assaysua1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000087sensitive to hydrogen peroxide131
expressivity FYPO_EXT:0000003Cell growth assaysua1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
Cell growth assaysua1ΔNullPECO:0000137PMID:18665268
FYPO:0002060viable vegetative cell populationMicroscopysua1ΔNullPMID:204732893759
Microscopysua1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002751decreased sulfate importSubstance quantification evidenceSeR-2Not specifiedPMID:147232231
FYPO:0002476viable vacuolated vegetative cell, abnormal cell shape, normal cell sizeMicroscopysua1ΔNullPECO:0000005, PECO:0000137PMID:2369780614
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
115731001571423

UTRs

Region Coordinates Reference
five_prime_UTR1573100..1572958PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14306 Pfam IPR025980 ATP-sulfurylase PUA-like domain 6 159 1
PF01747 Pfam IPR024951 Sulphate adenylyltransferase catalytic domain 167 385 1
PTHR11055 HMMPANTHER 5 298 2
PTHR11055:SF2 HMMPANTHER 5 298 1
3.40.50.620 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 181 389 39
3.40.50.300 Gene3D 390 467 288
3.10.400.10 Gene3D 7 180 1
SSF52374 SuperFamily 167 386 24
SSF88697 SuperFamily IPR015947 PUA-like domain 6 166 8
TIGR00339 tigrfam IPR002650 Sulphate adenylyltransferase 6 382 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.74 Da
Charge 3.50
Isoelectric point 7.14
Molecular weight 54.75 kDa
Number of residues 490
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00047O-phospho-L-threonineIDAT356PMID:18257517692
present during cellular response to thiabendazole
MOD:00696phosphorylated residueNASPMID:182575171922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
603058during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
567856during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
585821during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
582513during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
574335during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
140361.25during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
28115.01during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
78during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
4.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae MET3full PMID:18388974
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
sck2serine/threonine protein kinase Sck2 Phenotypic EnhancementPMID:24463365
Positive Genetic
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
iwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
mug81ATP-dependent RNA helicase Slh1 (predicted) Positive GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
jmj2histone demethylase Jmj2 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
sua1sulfate adenylyltransferase Two-hybridPMID:23695164
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPBC27.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC27.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC27.08c BioGRID Interaction Datasets
Expression Viewer SPBC27.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC27.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC27.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC27.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC27.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC27.08c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC27.08c Cell Cycle Data
GEO SPBC27.08c GEO profiles
PInt SPBC27.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC27.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC27.08c Fission yeast phenotypic data & analysis
SPD / RIKEN23/23G03Orfeome Localization Data
IntEnz2.7.7.4Integrated relational Enzyme database
Rhea2.7.7.4Annotated reactions database
UniProtKB/SwissProtP78937Sulfate adenylyltransferase
ModBaseP78937Database of comparative protein structure models
STRINGP78937Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595662sulfate adenylyltransferase
RefSeq mRNANM_001021556972h- sulfate adenylyltransferase (sua1), mRNA
European Nucleotide ArchiveAAN32720.1ENA Protein Mapping
European Nucleotide ArchiveBAA12186.1ENA Protein Mapping
European Nucleotide ArchiveCAB89007.1ENA Protein Mapping
UniParcUPI000012EF49UniProt Archive

Literature for sua1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014