tri1 (SPBC29A10.09c)

Gene Standard Nametri1 Characterisation Statuspublished
Systematic IDSPBC29A10.09c Feature Typeprotein coding
Synonyms Name Description
Producttriman, ribonuclease involved in priRNA formation Tri1 Product Size427aa, 49.01 kDa
Genomic Location Chromosome II, 2557200-2554177 (3024nt); CDS:2556947-2555664 (1284nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
heterochromatin assembly involved in chromatin silencing by small RNA2
Annotation ExtensionEvidenceWith/FromReference
ncRNA 3'-end processing30
Annotation ExtensionEvidenceWith/FromReference
RNA phosphodiester bond hydrolysis, exonucleolytic22
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopytri1ΔNullPMID:204732893759
Microscopytri1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003098abnormal heterochromatin assembly at centromere outer repeatCell growth assaytri1ΔNullPMID:240952771
FYPO:0002064abolished 3'-5'-exoribonuclease activityEnzyme assay dataD28A (D28A)Not specifiedPMID:240952773
FYPO:0003094decreased centromeric outer repeat transcript levelTranscript expression level evidencetri1ΔNullPMID:240952778
FYPO:0003101decreased heterochromatin assembly at protein coding gene2
affecting SPAC8C9.04Chromatin immunoprecipitation experimenttri1ΔNullPMID:24095277
affecting mcp5Chromatin immunoprecipitation experimenttri1ΔNullPMID:24095277
FYPO:0003096decreased histone H3-K9 methylation at centromere outer repeatChromatin immunoprecipitation experimenttri1ΔNullPMID:240952777
FYPO:0003103decreased mRNA-derived small RNA level1
affecting SPAC8C9.04Transcript expression level evidencetri1ΔNullPMID:24095277
affecting mcp5Transcript expression level evidencetri1ΔNullPMID:24095277
FYPO:0002134decreased RNA binding10
affecting ago1Gel electrophoresis evidencetri1ΔNullPMID:24095277
FYPO:0003093increased Argonaute-associated RNA lengthGel electrophoresis evidencetri1ΔNullPMID:240952771
FYPO:0003100normal heterochromatin assembly at protein coding gene1
affecting mei4Chromatin immunoprecipitation experimenttri1ΔNullPMID:24095277
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopytri1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04857 Pfam IPR006941 Ribonuclease CAF1 1 396 2
PTHR15092 HMMPANTHER 1 416 1
PTHR15092:SF9 HMMPANTHER 1 416 1
3.30.420.10 Gene3D 257 407 32
3.30.420.10 Gene3D 2 128 32
SSF53098 SuperFamily IPR012337 Ribonuclease H-like domain 259 350 34
SSF53098 SuperFamily IPR012337 Ribonuclease H-like domain 378 410 34
SSF53098 SuperFamily IPR012337 Ribonuclease H-like domain 1 130 34

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.77 Da
Charge 8.50
Isoelectric point 8.14
Molecular weight 49.01 kDa
Number of residues 427
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.19during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.18during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
no apparent S. cerevisiae ortholog602
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
ago1argonaute Affinity Capture-MSPMID:24095277
Reconstituted Complex
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rrp6exosome 3'-5' exoribonuclease subunit Rrp6 (predicted) Phenotypic SuppressionPMID:24095277
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Positive GeneticPMID:22681890
swd3WD repeat protein Swd3 Negative GeneticPMID:22681890
dcr1dicer Phenotypic EnhancementPMID:24095277
Phenotypic Suppression
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC29A10.09c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC29A10.09c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC29A10.09c BioGRID Interaction Datasets
Expression Viewer SPBC29A10.09c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC29A10.09c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC29A10.09c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC29A10.09c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC29A10.09c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC29A10.09c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC29A10.09c Cell Cycle Data
GEO SPBC29A10.09c GEO profiles
PInt SPBC29A10.09c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC29A10.09c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC29A10.09c Fission yeast phenotypic data & analysis
SPD / RIKEN51/51D05Orfeome Localization Data
UniProtKB/SwissProtO94386Uncharacterized protein C29A10.09c
ModBaseO94386Database of comparative protein structure models
STRINGO94386Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596054CAF1 family ribonuclease (predicted)
RefSeq mRNANM_001021965972h- CAF1 family ribonuclease (predicted) (SPBC29A10.09c), mRNA
European Nucleotide ArchiveCAA22437.1ENA Protein Mapping
UniParcUPI000006A184UniProt Archive

Literature for tri1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014