orc1 (SPBC29A10.15)

Gene Standard Nameorc1 ChromosomeII
Systematic IDSPBC29A10.15 Gene Start2571077
Synonymscdc30, orp1 Gene End2573477
Productorigin recognition complex subunit Orc1 Gene Length2401
Feature Typeprotein coding CDS Start2571162
Name Description CDS End2573337
Characterisation Statuspublished CDS Length2176

Ensembl Gene Location
  1. vps902
  2. SPBC29A10.12
  3. atp7
  4. prl25
  5. rec8
  6. orc1
  7. SPNCRNA.1529
  8. SPBC29A10.17
  9. SPBC29A10.16c
  10. SPBTRNAPHE.03
  11. SPBC2G5.01
  12. ckb2
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000062abnormal nuclear morphologyMicroscopyorc1::ura4+ (deletion)deletionPMID:8618924
has_penetrance FYPO_EXT:0000003Microscopyorc1delta (deletion)deletionPMID:8895665
FYPO:0000173abnormal mitotic cell cycle DNA replication checkpointMicroscopycdc30-4 (unknown)unknownPECO:0000004PMID:8895665
FYPO:0000217abnormal DNA replicationGel electrophoresis evidencecdc30-4 (unknown)unknownNot specifiedPECO:0000004PMID:8895665
FYPO:0000229cutFlow cytometry dataorp1-4 (unknown)unknownNot specifiedPECO:0000004PMID:9392077
Microscopyorc1::ura4+ (deletion)deletionPMID:8618924
Microscopycdc30-4 (unknown)unknownPECO:0000004PMID:8895665
FYPO:0000344enlarged nucleusMicroscopynot recorded (wild type)wild_typeOverexpressionPMID:8895665
FYPO:0000395mitotic cell cycle arrest at G1/S phase transitionFlow cytometry datacdc30-4 (unknown)unknownPECO:0000004PMID:8895665
FYPO:0000760normal conjugationCell growth assaycdc30-4 (unknown)unknownPECO:0000004PMID:8895665
FYPO:0000838normal protein localization to nucleusProtein localization experimentorp1-4 (unknown)unknownNot specifiedPECO:0000004PMID:10588642
assayed_using mcm2
assayed_using mcm4Protein localization experimentorp1-4 (unknown)unknownNot specifiedPECO:0000004PMID:10747035
FYPO:0000839elongated mononucleate aseptate cellMicroscopynot recorded (wild type)wild_typeOverexpressionPMID:8895665
Microscopycdc30-4 (unknown)unknownPECO:0000004PMID:8895665
Microscopyorc1::ura4+ (deletion)deletionPMID:8618924
FYPO:0001387loss of viability at high temperatureCell growth assayorp1-4 (unknown)unknownNot specifiedPECO:0000004PMID:9914167
FYPO:0001430cell cycle arrest with unreplicated DNAFlow cytometry dataorp1-4 (unknown)unknownNot specifiedPECO:0000004PMID:9392077
FYPO:0001508abolished protein localization to chromatin during vegetative growthProtein localization experimentorp1-4 (unknown)unknownNot specifiedPECO:0000004PMID:10747035
assayed_using mcm4
FYPO:0002061inviable vegetative cell populationMicroscopyorc1delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0003688DNA replication origin bindingIDAPMID:11850415
contributes_toIDAPMID:11850415
GO:0005524ATP bindingISMPFAM:PF00004GO_REF:0000001
ISSUniProtKB:P54784GO_REF:0000024
GO:0016887ATPase activityISSUniProtKB:P54784GO_REF:0000024
ISMPFAM:PF00004GO_REF:0000001
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0006261DNA-dependent DNA replicationIMPPMID:8618924
GO:0006270DNA replication initiationIMPPMID:8895665
GO:0033314mitotic DNA replication checkpoint
requiredIMPPMID:11988741
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0000790nuclear chromatin
constitutiveIDAPMID:16199868
has_part origin_of_replicationIDAPMID:10490657
GO:0005634nucleusIDAPMID:8895665
IDAPMID:16823372
GO:0005656pre-replicative complexICGO:0005664PMID:10535928
GO:0005664nuclear origin of replication recognition complexIDAPMID:11850415
IDAPMID:10535928
GO:0031261DNA replication preinitiation complexICGO:0005664PMID:10535928
GO:0043596nuclear replication forkICGO:0005664PMID:10535928
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
125710772572048
225721012573477
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF01426 IPR001025 Pfam Bromo adjacent homology (BAH) domain 63 179 3
PF00004 IPR003959 Pfam ATPase, AAA-type, core 365 507 32
SM00384 IPR017956 SMART AT hook, DNA-binding motif 225 237 5
SM00382 IPR003593 SMART AAA+ ATPase domain 360 511 73
SM00439 IPR001025 SMART Bromo adjacent homology (BAH) domain 62 190 3
PS51038 IPR001025 Prosite Profiles Bromo adjacent homology (BAH) domain 62 193 3
G3DSA:1.10.8.60 Gene3D 503 589 34
G3DSA:3.40.50.300 Gene3D 340 502 279
SSF52540 SuperFamily 326 586 294
Coil ncoils Rabaptin coiled-coil domain 202 223 974
PTHR10763 hmmpanther 7 707 2
PTHR10763:SF6 IPR020793 hmmpanther 7 707 1

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:18257517

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539
PBO:0002147AT hook proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002147

Protein Properties

Ave. residue weight 113.88 Da
Charge 7.50
Isoelectric point 7.40
Molecular weight 80.51 kDa
Number of residues 707
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
constitutive PMID:8618924
protein levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide554.73PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.49PMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide1.6PMID:23101633
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
mrc1mediator of replication checkpoint 1 Phenotypic EnhancementPMID:18667534
Synthetic Growth DefectPMID:16849602
mcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:11606526
dpb4DNA polymerase epsilon subunit Dpb4 Synthetic LethalityPMID:15388803
rad1checkpoint clamp complex protein Rad1 Phenotypic EnhancementPMID:18667534
Phenotypic EnhancementPMID:12186947
Phenotypic Suppression
rad9checkpoint clamp complex protein Rad9 Phenotypic EnhancementPMID:18667534
brc1BRCT domain protein Brc1 Phenotypic SuppressionPMID:22366461
Synthetic Growth Defect
rad17RFC related checkpoint protein Rad17 Phenotypic EnhancementPMID:18667534
Phenotypic SuppressionPMID:12186947
Phenotypic Enhancement
crb2DNA repair protein Rad9 homolog, Rhp9 Phenotypic EnhancementPMID:18667534
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:12186947
cds1replication checkpoint kinase Cds1 Synthetic RescuePMID:16849602
Phenotypic EnhancementPMID:18667534
rad3ATR checkpoint kinase Rad3 Phenotypic EnhancementPMID:18667534
Phenotypic SuppressionPMID:12186947
Phenotypic Enhancement
mst1KAT5 family histone acetyltransferase Mst1 Synthetic Growth DefectPMID:18505873
mus81Holliday junction resolvase subunit Mus81 Phenotypic EnhancementPMID:18667534
spb70DNA polymerase alpha B-subunit Synthetic Growth DefectPMID:15314153
swi1replication fork protection complex subunit Swi1 Phenotypic EnhancementPMID:18667534
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Phenotypic EnhancementPMID:12186947
Phenotypic Suppression
Phenotypic EnhancementPMID:18667534
cbp1CENP-B homolog Synthetic Growth DefectPMID:17112379
cig2G1/S-specific B-type cyclin Cig2 Synthetic RescuePMID:15194812
mcb1MCM binding protein homolog Mcb1 (predicted) Synthetic Growth DefectPMID:23322785
rum1CDK inhibitor Rum1 Dosage RescuePMID:15194812
Phenotypic SuppressionPMID:10388806
chk1Chk1 protein kinase Phenotypic EnhancementPMID:12186947
Synthetic Growth DefectPMID:16849602
Phenotypic EnhancementPMID:18667534
Synthetic Growth DefectPMID:21712547
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic Growth DefectPMID:11027263
rqh1RecQ type DNA helicase Rqh1 Phenotypic EnhancementPMID:18667534
wee1M phase inhibitor protein kinase Wee1 Phenotypic EnhancementPMID:12186947
rad2414-3-3 protein Rad24 Phenotypic EnhancementPMID:18667534
cdc18MCM loader Phenotypic EnhancementPMID:12186947
Phenotypic SuppressionPMID:8895665
Phenotypic Enhancement
Phenotypic EnhancementPMID:11988741
rid1GTPase binding protein Rid1 (predicted) Dosage RescuePMID:20799962
swi3replication fork protection complex subunit Swi3 Phenotypic EnhancementPMID:18667534
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
spb70DNA polymerase alpha B-subunit Affinity Capture-WesternPMID:15314153
orc6origin recognition complex subunit Orc6 Reconstituted ComplexPMID:10535928
Reconstituted ComplexPMID:11850415
orc3origin recognition complex subunit Orp3 Reconstituted ComplexPMID:11850415
mcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:8895665
orc2origin recognition complex subunit Orc2 Affinity Capture-WesternPMID:10535928
Reconstituted Complex
Affinity Capture-WesternPMID:10523506
Affinity Capture-WesternPMID:11683912
Reconstituted ComplexPMID:11717425
Co-purification
Reconstituted ComplexPMID:11850415
ark1aurora-B kinase Ark1 Biochemical ActivityPMID:21712547
orc4origin recognition complex subunit Orc4 Reconstituted ComplexPMID:10535928
Reconstituted ComplexPMID:11850415
Affinity Capture-WesternPMID:11683912
Reconstituted ComplexPMID:11717425
Reconstituted ComplexPMID:11689699
Reconstituted ComplexPMID:12840006
cdc23MCM-associated protein Mcm10 Two-hybridPMID:12185500
cdc45DNA replication pre-initiation complex subunit Cdc45 Co-localizationPMID:15338237
cdc18MCM loader Reconstituted ComplexPMID:11850415
Affinity Capture-WesternPMID:8895665
orc5origin recognition complex subunit Orc5 Affinity Capture-WesternPMID:10535928
Reconstituted Complex
Affinity Capture-WesternPMID:10523506
Reconstituted ComplexPMID:11850415
Co-purificationPMID:11689699
Affinity Capture-WesternPMID:11683912
Reconstituted ComplexPMID:18723846
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External References
Database Identifier Description
NBRP SPBC29A10.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC29A10.15 Retrieval of eukaryotic orthologs
BioGrid SPBC29A10.15 BioGRID Interaction Datasets
Bähler Lab SPBC29A10.15 Cell Cycle Expression Profile
Bähler Lab SPBC29A10.15 Meiosis/Sporulation Expression Profies
Bähler Lab SPBC29A10.15 Pheromone response/mating expression profiles
Bähler Lab SPBC29A10.15 Environmental stress expression profiles
Bähler Lab SPBC29A10.15 Bähler Lab Transcriptome Viewer
Cyclebase SPBC29A10.15 Cell Cycle Data
PInt SPBC29A10.15 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPBC29A10.15 Entrez Gene
WikiGene2540470origin recognition complex subunit Orc1
Uniprot_genenameORC1
EntrezGene2540470origin recognition complex subunit Orc1
SPD / RIKEN51/51E07Orfeome Localization Data
UniProtKB/SwissProtP54789Origin recognition complex subunit 1
ModBaseP54789Database of comparative protein structure models
Pfam Protein DomainsP54789Pfam Domain Arrangement
RefSeq PeptideNP_596060origin recognition complex subunit Orc1
RefSeq mRNANM_001021971972h- origin recognition complex subunit Orc1 (orc1), mRNA
European Nucleotide ArchiveAAB38247ENA Protein Mapping
European Nucleotide ArchiveAAC49129ENA Protein Mapping
European Nucleotide ArchiveAAC49141ENA Protein Mapping
European Nucleotide ArchiveCAA22443ENA Protein Mapping
UniParcUPI0000130E58UniProt Archive

Literature for orc1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013