isp4 (SPBC29B5.02c)


Gene Standard Nameisp4 Characterisation Statuspublished
Systematic IDSPBC29B5.02c Feature Typeprotein coding
Synonyms Name Description
ProductOPT oligopeptide transmembrane transporter family Isp4 Product Size785aa, 89.19 kDa
Genomic Location Chromosome II, 1553467-1550293 (3175nt); CDS:1552791-1550434 (2358nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
tetrapeptide transmembrane transporter activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
tetrapeptide transmembrane transport1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
abolished cell population growth on tetrapeptide nitrogen sourceisp4ΔNull2
viable vegetative cell populationisp4ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologyisp4ΔNull3094
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in bhd1 folliculin/Birt-Hogg-Dube syndrome ortholog Bhd1
FYPO affected by mutation in tsc1 hamartin
FYPO affected by mutation in tsc2 tuberin
FYPO affected by mutation in ubr11 UBR ubiquitin-protein ligase E3 Ubr11
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1553467..1550293
mRNA1553467..1550293
5' UTR1553467..1552792PMID:18488015
CDS1552791..1550434
3' UTR1550433..1550293AU008626
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03169 Pfam IPR004813 Oligopeptide transporter, OPT superfamily 89 740 3
TMhelix TMHMM 92 114 959
TMhelix TMHMM 462 484 959
TMhelix TMHMM 222 244 959
TMhelix TMHMM 685 707 959
TMhelix TMHMM 643 662 959
TMhelix TMHMM 338 360 959
TMhelix TMHMM 264 286 959
TMhelix TMHMM 411 433 959
TMhelix TMHMM 536 558 959
TMhelix TMHMM 720 742 959
TMhelix TMHMM 196 218 959
TMhelix TMHMM 579 596 959
TMhelix TMHMM 611 630 959
TMhelix TMHMM 491 513 959
PTHR22601 HMMPANTHER 7 785 3
PTHR22601:SF7 HMMPANTHER 7 785 1
TIGR00727 tigrfam IPR004648 Tetrapeptide transporter, OPT1/isp4 67 748 3
TIGR00728 tigrfam IPR004813 Oligopeptide transporter, OPT superfamily 73 743 3

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 113.62 Da
Charge -3.50
Isoelectric point 5.99
Molecular weight 89.19 kDa
Number of residues 785
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS26
present during mitotic M phaseS37
present during mitotic M phaseS30
present during mitotic M phaseS40
present during mitotic M phaseS19
present during mitotic M phaseS4
S8
present during mitotic M phaseS35
present during mitotic M phaseS8
present during mitotic M phaseS17
present during mitotic M phaseS42
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S4 PMID:21712547
experimental evidence S8 PMID:24763107
present during mitotic M phase experimental evidence S8 PMID:21712547
present during mitotic M phase experimental evidence S17 PMID:21712547
present during mitotic M phase experimental evidence S19 PMID:21712547
present during mitotic M phase experimental evidence S26 PMID:21712547
present during mitotic M phase experimental evidence S30 PMID:21712547
present during mitotic M phase experimental evidence S35 PMID:21712547
present during mitotic M phase experimental evidence S37 PMID:21712547
present during mitotic M phase experimental evidence S40 PMID:21712547
present during mitotic M phase experimental evidence S42 PMID:21712547
O4'-phospho-L-tyrosine 87
present during mitotic M phaseY32
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence Y32 PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA leveldecreased in presence of cadmium sulfate expression microarray evidencePMID:12529438
decreased during GO:0034605expression microarray evidencePMID:12529438
decreased during GO:0071470expression microarray evidencePMID:12529438
decreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased in presence of purvalanol A expression microarray evidencePMID:22840777
absent during GO:0072690Northern assay evidencePMID:7954893
increased during GO:0006279Northern assay evidencePMID:7954893
decreased during GO:0034599expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3433during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3422during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3473during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3032during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3104during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
26065.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
15during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
positive genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:22681890
positive genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
negative genetic interaction withmug81ATP-dependent RNA helicase Slh1, human ASCC3 ortholog (predicted) Negative GeneticPMID:22681890
synthetic growth defect withptr2PTR family peptide transmembrane transporter Ptr2 Synthetic Growth DefectPMID:22226946
negative genetic interaction withsdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:22681890
positive genetic interaction withssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Positive GeneticPMID:22681890
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC29B5.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC29B5.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC29B5.02c BioGRID Interaction Datasets
Expression Viewer SPBC29B5.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC29B5.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC29B5.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC29B5.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC29B5.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC29B5.02c Transcriptome Viewer (Bähler Lab)
GEO SPBC29B5.02c GEO profiles
PInt SPBC29B5.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC29B5.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC29B5.02c Fission yeast phenotypic data & analysis
Cyclebase SPBC29B5.02c.1 Cell Cycle Data
SPD / RIKEN33/33G09Orfeome Localization Data
UniProtKB/SwissProtP40900Sexual differentiation process protein isp4
ModBaseP40900Database of comparative protein structure models
STRINGP40900Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595653OPT oligopeptide transporter family Isp4
RefSeq mRNANM_001021547972h- OPT oligopeptide transporter family Isp4 (isp4), mRNA
European Nucleotide ArchiveD14061ENA EMBL mapping
European Nucleotide ArchiveBAA12193.1ENA Protein Mapping
European Nucleotide ArchiveBAA87199.1ENA Protein Mapping
European Nucleotide ArchiveCAC05511.1ENA Protein Mapping
UniParcUPI000012D907UniProt Archive

Literature for isp4

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015