isp4 (SPBC29B5.02c)

Gene Standard Nameisp4 Characterisation Statuspublished
Systematic IDSPBC29B5.02c Feature Typeprotein coding
Synonyms Name Description
ProductOPT oligopeptide transporter family Isp4 Product Size785aa, 89.19 kDa
Genomic Location Chromosome II, 1553467-1550293 (3175nt); CDS:1552791-1550434 (2358nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
tetrapeptide transmembrane transporter activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
tetrapeptide transmembrane transport1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001577abolished cell population growth on tetrapeptide nitrogen sourceCell growth assayisp4ΔNullPMID:222269462
Cell growth assayisp4ΔNullPMID:9643541
FYPO:0002060viable vegetative cell populationCell growth assayisp4ΔNullPMID:96435413759
Cell growth assayisp4ΔNullPECO:0000201PMID:7954893
Microscopyisp4ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyisp4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
FYPO affected by mutation in ubr11 UBR ubiquitin-protein ligase E3 Ubr11 PMID:22226946
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03169 Pfam IPR004813 Oligopeptide transporter, OPT superfamily 89 740 3
TMhelix TMHMM 720 742 959
TMhelix TMHMM 92 114 959
TMhelix TMHMM 264 286 959
TMhelix TMHMM 462 484 959
TMhelix TMHMM 579 596 959
TMhelix TMHMM 491 513 959
TMhelix TMHMM 411 433 959
TMhelix TMHMM 222 244 959
TMhelix TMHMM 643 662 959
TMhelix TMHMM 611 630 959
TMhelix TMHMM 196 218 959
TMhelix TMHMM 685 707 959
TMhelix TMHMM 536 558 959
TMhelix TMHMM 338 360 959
PTHR22601 HMMPANTHER 9 785 3
TIGR00728 tigrfam IPR004813 Oligopeptide transporter, OPT superfamily 73 743 3
TIGR00727 tigrfam IPR004648 Tetrapeptide transporter, OPT1/isp4 67 748 3

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 113.62 Da
Charge -3.50
Isoelectric point 5.99
Molecular weight 89.19 kDa
Number of residues 785

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS35PMID:217125471670
present during mitotic M phase
present during mitotic M phaseexperimental evidenceS8PMID:21712547
present during mitotic M phaseexperimental evidenceS40PMID:21712547
present during mitotic M phaseexperimental evidenceS19PMID:21712547
present during mitotic M phaseexperimental evidenceS26PMID:21712547
present during mitotic M phaseexperimental evidenceS30PMID:21712547
present during mitotic M phaseexperimental evidenceS37PMID:21712547
present during mitotic M phaseexperimental evidenceS42PMID:21712547
present during mitotic M phaseexperimental evidenceS17PMID:21712547
present during mitotic M phaseexperimental evidenceS4PMID:21712547
experimental evidenceS8PMID:24763107
MOD:00048O4'-phospho-L-tyrosineexperimental evidenceY32PMID:2171254785
present during mitotic M phase
Gene Expression

Qualitative Gene Expression

RNA leveldecreased during GO:0034605expression microarray evidencePMID:12529438
decreased during GO:0071470expression microarray evidencePMID:12529438
decreased during GO:0034599expression microarray evidencePMID:12529438
decreased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased during GO:0006279Northern assay evidencePMID:7954893
absent during GO:0072690Northern assay evidencePMID:7954893
decreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased in presence of purvalanol A expression microarray evidencePMID:22840777

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3433during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
3422during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
3473during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
3032during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
3104during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
26065.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
15during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
conserved in fungi4600
conserved in eukaryotes4514

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
kin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Positive GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
ptr2PTR family peptide transporter Ptr2 Synthetic Growth DefectPMID:22226946
mug81ATP-dependent RNA helicase Slh1 (predicted) Negative GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:22681890
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC29B5.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC29B5.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC29B5.02c BioGRID Interaction Datasets
Expression Viewer SPBC29B5.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC29B5.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC29B5.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC29B5.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC29B5.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC29B5.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC29B5.02c Cell Cycle Data
GEO SPBC29B5.02c GEO profiles
PInt SPBC29B5.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC29B5.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC29B5.02c Fission yeast phenotypic data & analysis
SPD / RIKEN33/33G09Orfeome Localization Data
UniProtKB/SwissProtP40900Sexual differentiation process protein isp4
ModBaseP40900Database of comparative protein structure models
STRINGP40900Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595653OPT oligopeptide transporter family Isp4
RefSeq mRNANM_001021547972h- OPT oligopeptide transporter family Isp4 (isp4), mRNA
European Nucleotide ArchiveD14061ENA EMBL mapping
European Nucleotide ArchiveBAA12193.1ENA Protein Mapping
European Nucleotide ArchiveBAA87199.1ENA Protein Mapping
European Nucleotide ArchiveCAC05511.1ENA Protein Mapping
UniParcUPI000012D907UniProt Archive

Literature for isp4

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014