isp4 (SPBC29B5.02c)

Gene Standard Nameisp4 Characterisation Statuspublished
Systematic IDSPBC29B5.02c Feature Typeprotein coding
Synonyms Name Description
ProductOPT oligopeptide transporter family Isp4 Product Size785aa, 89.19 kDa
Genomic Location Chromosome II, 1553467-1550293 (3175nt); CDS:1552791-1550434 (2358nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1901584tetrapeptide transmembrane transporter activityIMPPMID:222269461
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000747conjugation with cellular fusionIMPPMID:795489365
GO:0031142induction of conjugation upon nitrogen starvationIEPPMID:795489311
GO:1901583tetrapeptide transmembrane transportIMPPMID:222269461
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005794Golgi apparatus355
during cellular response to nitrogen levelsIDAPMID:22226946
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371016
GO:0005886plasma membrane243
during cellular response to nitrogen starvationIDAPMID:22226946
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001577abolished cell population growth on tetrapeptide nitrogen sourceCell growth assayisp4ΔNullPMID:222269462
FYPO:0002060viable vegetative cell populationMicroscopyisp4ΔNullPMID:204732893755
Microscopyisp4ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopyisp4ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Start End Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03169 Pfam IPR004813 Oligopeptide transporter, OPT superfamily 89 740 3
TMhelix TMHMM 536 558 959
TMhelix TMHMM 338 360 959
TMhelix TMHMM 611 630 959
TMhelix TMHMM 196 218 959
TMhelix TMHMM 685 707 959
TMhelix TMHMM 491 513 959
TMhelix TMHMM 411 433 959
TMhelix TMHMM 222 244 959
TMhelix TMHMM 643 662 959
TMhelix TMHMM 264 286 959
TMhelix TMHMM 462 484 959
TMhelix TMHMM 579 596 959
TMhelix TMHMM 720 742 959
TMhelix TMHMM 92 114 959
PTHR22601 HMMPANTHER 9 785 3
Low complexity (SEG) seg 37 51
TIGR00728 tigrfam IPR004813 Oligopeptide transporter, OPT superfamily 73 743 3
TIGR00727 tigrfam IPR004648 Tetrapeptide transporter, OPT1/isp4 67 748 3

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 113.62 Da
Charge -3.50
Isoelectric point 5.99
Molecular weight 89.19 kDa
Number of residues 785
Gene Expression

Qualitative Gene Expression

RNA leveldecreasedexpression microarray evidencePMID:12529438
during GO:0034605
decreasedexpression microarray evidencePMID:12529438
during GO:0071470
in presence of methyl methanesulfonate
expression microarray evidencePMID:12529438
in presence of purvalanol A
expression microarray evidencePMID:22840777
in presence of cadmium sulfate
expression microarray evidencePMID:12529438
decreasedexpression microarray evidencePMID:12529438
during GO:0034599

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
26065.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
15during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
conserved in fungi4564
conserved in eukaryotes4482

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
kin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Positive GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
ptr2PTR family peptide transporter Ptr2 Synthetic Growth DefectPMID:22226946
mug81ATP-dependent RNA helicase Slh1 (predicted) Negative GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:22681890
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC29B5.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC29B5.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC29B5.02c BioGRID Interaction Datasets
Expression Viewer SPBC29B5.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC29B5.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC29B5.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC29B5.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC29B5.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC29B5.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC29B5.02c Cell Cycle Data
GEO SPBC29B5.02c GEO profiles
PInt SPBC29B5.02c Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN33/33G09Orfeome Localization Data
WikiGene2540364OPT oligopeptide transporter family
EntrezGene2540364OPT oligopeptide transporter family
UniProtKB/SwissProtP40900Sexual differentiation process protein isp4
ModBaseP40900Database of comparative protein structure models
StringP40900Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595653OPT oligopeptide transporter family
RefSeq mRNANM_001021547972h- OPT oligopeptide transporter family (isp4), mRNA
European Nucleotide ArchiveBAA03147ENA Protein Mapping
European Nucleotide ArchiveBAA12193ENA Protein Mapping
European Nucleotide ArchiveBAA87199ENA Protein Mapping
European Nucleotide ArchiveCAC05511ENA Protein Mapping
UniParcUPI000012D907UniProt Archive

Literature for isp4

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014