isp4 (SPBC29B5.02c)


Gene Standard Nameisp4 Characterisation Statuspublished
Systematic IDSPBC29B5.02c Feature Typeprotein coding
Synonyms Name Description
ProductOPT oligopeptide transporter family Isp4 Product Size785aa, 89.19 kDa
Genomic Location Chromosome II, 1553467-1550293 (3175nt); CDS:1552791-1550434 (2358nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1901584tetrapeptide transmembrane transporter activityIMPPMID:222269461
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1901583tetrapeptide transmembrane transportIMPPMID:222269461
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005794Golgi apparatus355
during cellular response to nitrogen levelsIDAPMID:22226946
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371017
GO:0005886plasma membrane242
during cellular response to nitrogen starvationIDAPMID:22226946
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001577abolished cell population growth on tetrapeptide nitrogen sourceCell growth assayisp4ΔNullPMID:222269462
FYPO:0002060viable vegetative cell populationMicroscopyisp4ΔNullPMID:204732893760
Cell growth assayisp4ΔNullPECO:0000201PMID:7954893
Microscopyisp4ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopyisp4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in ubr11 UBR ubiquitin ligase Ubr11 PMID:22226946
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
115534671550293

UTRs

Region Coordinates Reference
five_prime_UTR1553467..1552792PMID:18488015
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03169 Pfam IPR004813 Oligopeptide transporter, OPT superfamily 89 740 3
TMhelix TMHMM 720 742 959
TMhelix TMHMM 92 114 959
TMhelix TMHMM 264 286 959
TMhelix TMHMM 462 484 959
TMhelix TMHMM 579 596 959
TMhelix TMHMM 491 513 959
TMhelix TMHMM 222 244 959
TMhelix TMHMM 411 433 959
TMhelix TMHMM 643 662 959
TMhelix TMHMM 611 630 959
TMhelix TMHMM 196 218 959
TMhelix TMHMM 685 707 959
TMhelix TMHMM 536 558 959
TMhelix TMHMM 338 360 959
PTHR22601:SF7 HMMPANTHER 7 785 1
PTHR22601 HMMPANTHER 7 785 3
TIGR00728 tigrfam IPR004813 Oligopeptide transporter, OPT superfamily 73 743 3
TIGR00727 tigrfam IPR004648 Tetrapeptide transporter, OPT1/isp4 67 748 3

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 113.62 Da
Charge -3.50
Isoelectric point 5.99
Molecular weight 89.19 kDa
Number of residues 785
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS19PMID:217125471663
present during mitotic M phase
present during mitotic M phaseS26PMID:21712547
present during mitotic M phaseS30PMID:21712547
present during mitotic M phaseS37PMID:21712547
present during mitotic M phaseS4PMID:21712547
present during mitotic M phaseS35PMID:21712547
present during mitotic M phaseS17PMID:21712547
present during mitotic M phaseS42PMID:21712547
S8PMID:24763107
present during mitotic M phaseS8PMID:21712547
present during mitotic M phaseS40PMID:21712547
MOD:00048O4'-phospho-L-tyrosineY32PMID:2171254781
present during mitotic M phase
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0006279Northern assay evidencePMID:7954893
absent during GO:0072690Northern assay evidencePMID:7954893
increased in presence of purvalanol A expression microarray evidencePMID:22840777
decreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
decreased during GO:0071470expression microarray evidencePMID:12529438
decreased during GO:0034605expression microarray evidencePMID:12529438
decreased in presence of cadmium sulfate expression microarray evidencePMID:12529438
decreased during GO:0034599expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
26065.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
3104during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
NDduring GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
3433during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3422during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3473during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3032during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
15during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
kin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Positive GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
ptr2PTR family peptide transporter Ptr2 Synthetic Growth DefectPMID:22226946
mug81ATP-dependent RNA helicase Slh1 (predicted) Negative GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:22681890
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC29B5.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC29B5.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC29B5.02c BioGRID Interaction Datasets
Expression Viewer SPBC29B5.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC29B5.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC29B5.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC29B5.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC29B5.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC29B5.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC29B5.02c Cell Cycle Data
GEO SPBC29B5.02c GEO profiles
PInt SPBC29B5.02c Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN33/33G09Orfeome Localization Data
WikiGene2540364OPT oligopeptide transporter family
EntrezGene2540364OPT oligopeptide transporter family
UniProtKB/SwissProtP40900Sexual differentiation process protein isp4
ModBaseP40900Database of comparative protein structure models
STRINGP40900Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595653OPT oligopeptide transporter family
RefSeq mRNANM_001021547972h- OPT oligopeptide transporter family (isp4), mRNA
European Nucleotide ArchiveD14061ENA EMBL mapping
European Nucleotide ArchiveBAA03147ENA Protein Mapping
European Nucleotide ArchiveBAA12193ENA Protein Mapping
European Nucleotide ArchiveBAA12193.1ENA Protein Mapping
European Nucleotide ArchiveBAA87199ENA Protein Mapping
European Nucleotide ArchiveBAA87199.1ENA Protein Mapping
European Nucleotide ArchiveCAC05511ENA Protein Mapping
European Nucleotide ArchiveCAC05511.1ENA Protein Mapping
UniParcUPI000012D907UniProt Archive

Literature for isp4

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014