myo51 (SPBC2D10.14c)


Gene Standard Namemyo51 Characterisation Statuspublished
Systematic IDSPBC2D10.14c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type V Product Size1471aa, 167.66 kDa
Genomic Location Chromosome II, 2994929-2990178 (4752nt); CDS:2994814-2990305 (4510nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
calmodulin binding13
Annotation ExtensionEvidenceWith/FromReference
microfilament motor activity4
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin filament bundle organization14
Annotation ExtensionEvidenceWith/FromReference
formin-nucleated actin cable assembly2
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly27
Annotation ExtensionEvidenceWith/FromReference
regulation of actin filament organization involved in mitotic actomyosin contractile ring assembly1
Annotation ExtensionEvidenceWith/FromReference
regulation of formin-nucleated actin cable assembly1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring38
Annotation ExtensionEvidenceWith/FromReference
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
formin-nucleated actin cable2
Annotation ExtensionEvidenceWith/FromReference
myosin complex8
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationmyo51ΔNull3809
unnamed (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
abnormal actin cable morphology during mitosismyo51ΔNull1
abnormal medial cortical node migrationmyo51ΔNull1
abolished protein binding95
affecting myo51 and rng91-900 (901-1471)Not specified
abolished protein localization to actin cable1
affecting rng8myo51ΔNull
affecting rng9myo51ΔNull
cell lysis during cytokinesismyo51ΔNull8
decreased medial cortical node movement during contractile ring assemblymyo51ΔNull1
decreased rate of actin cable retrograde transportmyo51ΔNull2
decreased rate of actomyosin contractile ring assembly753-1471 (1-752)Not specified2
myo51ΔNull
elongated vegetative cellmyo51+ (wild type)Overexpression174
multiseptate vegetative cellmyo51ΔNull81
normal actin cable morphologymyo51ΔNull4
normal actin cortical patch localizationmyo51ΔNull18
normal protein binding56
affecting myo51 and rng91-1294 (1293-1471)Not specified
normal protein localization to actomyosin contractile ring14
affecting rng8myo51ΔNull
affecting rng9myo51ΔNull
normal protein localization to cell tip22
affecting myo52myo51ΔNull
affecting for3myo51ΔNull
normal rate of actin cable retrograde transportmyo51-ΔTail (C-terminal truncation)Not specified1
normal vegetative cell shapemyo51ΔNull24
viable vegetative cell with normal cell morphologymyo51ΔNull3094
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in for3 formin For3
FYPO affected by mutation in myo51 myosin type V
FYPO affected by mutation in rng8 contractile ring myosin V regulator Rng8
FYPO affected by mutation in rng9 contractile ring myosin V regulator Rng9
GO regulated by rng8 contractile ring myosin V regulator Rng8
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2994929..2994786, 2994730..2990810, 2990770..2990178
mRNA2994929..2990178
5' UTR2994929..2994815PMID:21511999
CDS2994814..2994786, 2994730..2990810, 2990770..2990305
3' UTR2990304..2990178PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 801 820 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 777 795 3
PF01843 Pfam IPR018444 Dil domain 1298 1399 3
PF00063 Pfam IPR001609 Myosin head, motor domain 69 737 5
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 850 869 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 754 773 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 870 892 3
SM00242 SMART IPR001609 Myosin head, motor domain 59 750 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 751 773 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 799 821 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 847 869 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 774 796 3
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 848 877 5
PS51456 Prosite Profiles IPR001609 Myosin head, motor domain 65 749 5
PS51126 Prosite Profiles IPR002710 Dilute 1171 1417 3
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 753 781 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 800 825 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 777 804 5
PTHR13140:SF289 HMMPANTHER 8 1119 1
PTHR13140 HMMPANTHER 8 1119 6
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 150 180 288
1.20.5.190 Gene3D 777 818 2
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 40 800 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 797 844 294
Coil ncoils Rabaptin coiled-coil domain 967 988 968
PR00193 PRINTS IPR001609 Myosin head, motor domain 434 462 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 487 515 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 152 177 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 201 228 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 95 114 5

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 113.98 Da
Charge 53.00
Isoelectric point 9.29
Molecular weight 167.66 kDa
Number of residues 1471
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1131during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
965during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1208during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
786during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1073during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1223.96during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
987.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.39during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesmyo1myosin type I Affinity Capture-RNAPMID:19805578
affinity capturesmyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity capturesmyo51myosin type V Affinity Capture-WesternPMID:24798735
affinity capturesmyo52myosin type V Affinity Capture-RNAPMID:19805578
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity capturesrng2IQGAP Affinity Capture-RNAPMID:19805578
affinity captured byrng3UCS-domain protein Rng3 Affinity Capture-RNAPMID:19098712
affinity capturesrng8contractile ring myosin V regulator Rng8 Affinity Capture-WesternPMID:24798735
affinity captured byrng8contractile ring myosin V regulator Rng8 Affinity Capture-WesternPMID:24798735
affinity capturesrng9contractile ring myosin V regulator Rng9 Affinity Capture-WesternPMID:24798735
affinity captured byrng9contractile ring myosin V regulator Rng9 Affinity Capture-WesternPMID:24798735
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withain1alpha-actinin Synthetic Growth DefectPMID:24798735
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
negative genetic interaction withbob1prefoldin subunit 5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcyk3cytokinesis protein Cyk3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcyp4cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp4 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withdao1D-amino acid oxidase Positive GeneticPMID:22681890
negative genetic interaction withelp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withftp105Ubp5 interacting protein Ftp105 Negative GeneticPMID:18818364
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
negative genetic interaction withlub1WD repeat protein Lub1 Negative GeneticPMID:18818364
phenotype enhanced bymal3EB1 family Mal3 Phenotypic EnhancementPMID:23051734
positive genetic interaction withmid1medial ring protein Mid1 Positive GeneticPMID:22681890
negative genetic interaction withmrc1mediator of replication checkpoint 1 Negative GeneticPMID:18818364
synthetic growth defect withmyo2myosin II heavy chain Synthetic Growth DefectPMID:24798735
enhances phenotype ofmyo2myosin II heavy chain Phenotypic EnhancementPMID:23185032
phenotype enhanced bymyo52myosin type V Phenotypic EnhancementPMID:23051734
synthetic growth defect withmyp2myosin II heavy chain Myo3 Synthetic Growth DefectPMID:24798735
positive genetic interaction withpit1serine/threonine protein kinase, meiotic Pit1 Positive GeneticPMID:22681890
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:18818364
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:18818364
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
negative genetic interaction withppm1leucine carboxyl methyltransferase, involved in regulation of autophagy Ppm1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
positive genetic interaction withpyp1tyrosine phosphatase Pyp1 Positive GeneticPMID:22681890
negative genetic interaction withrad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
synthetic growth defect withrlc1myosin II regulatory light chain Rlc1 Synthetic Growth DefectPMID:24798735
negative genetic interaction withSPBC30B4.06cmitochondrial GIDA family tRNA uridine 5-carboxymethylaminomethyl modification enzyme (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC2D10.14c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC2D10.14c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC2D10.14c BioGRID Interaction Datasets
Expression Viewer SPBC2D10.14c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC2D10.14c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC2D10.14c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC2D10.14c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC2D10.14c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC2D10.14c Transcriptome Viewer (Bähler Lab)
GEO SPBC2D10.14c GEO profiles
PInt SPBC2D10.14c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC2D10.14c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC2D10.14c Fission yeast phenotypic data & analysis
Cyclebase SPBC2D10.14c.1 Cell Cycle Data
SPD / RIKEN30/30H10Orfeome Localization Data
UniProtKB/SwissProtO74805Myosin-51
ModBaseO74805Database of comparative protein structure models
STRINGO74805Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596233myosin type V
RefSeq mRNANM_001022153972h- myosin type V (myo51), mRNA
European Nucleotide ArchiveCAA21172.1ENA Protein Mapping
UniParcUPI0000069F9DUniProt Archive

Literature for myo51

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015