myo51 (SPBC2D10.14c)


Gene Standard Namemyo51 Characterisation Statuspublished
Systematic IDSPBC2D10.14c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type V Product Size1471aa, 167.66 kDa
Genomic Location Chromosome II, 2994929-2990178 (4752nt); CDS:2994814-2990305 (4510nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
calmodulin binding10
Annotation ExtensionEvidenceWith/FromReference
microfilament motor activity4
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin filament bundle organization14
Annotation ExtensionEvidenceWith/FromReference
formin-nucleated actin cable assembly2
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly28
Annotation ExtensionEvidenceWith/FromReference
regulation of actin filament organization involved in mitotic actomyosin contractile ring assembly1
Annotation ExtensionEvidenceWith/FromReference
regulation of formin-nucleated actin cable assembly1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring32
Annotation ExtensionEvidenceWith/FromReference
cell division site299
Annotation ExtensionEvidenceWith/FromReference
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
formin-nucleated actin cable2
Annotation ExtensionEvidenceWith/FromReference
myosin complex8
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationmyo51ΔNull3781
not recorded (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
abnormal actin cable morphology during mitosismyo51ΔNull1
abnormal medial cortical node migrationmyo51ΔNull1
abolished protein binding68
affecting myo51 and rng91-900 (901-1471)Not specified
abolished protein localization to actin cable1
affecting rng8myo51ΔNull
affecting rng9myo51ΔNull
cell lysis during cytokinesis7
penetrance FYPO_EXT:0000003myo51ΔNull
decreased medial cortical node movement during contractile ring assemblymyo51ΔNull1
decreased rate of actin cable retrograde transportmyo51ΔNull2
decreased rate of actomyosin contractile ring assembly753-1471 (1-752)Not specified2
myo51ΔNull
elongated vegetative cellnot recorded (overexpression)703
multiseptate114
penetrance FYPO_EXT:0000003myo51ΔNull
normal actin cable morphologymyo51ΔNull4
normal actin cortical patch localizationmyo51ΔNull10
normal protein binding49
affecting myo51 and rng91-1294 (1293-1471)Not specified
normal protein localization to actomyosin contractile ring14
affecting rng8myo51ΔNull
affecting rng9myo51ΔNull
normal protein localization to cell tip17
affecting myo52myo51ΔNull
affecting for3myo51ΔNull
normal rate of actin cable retrograde transportmyo51-ΔTail (C-terminal truncation)Not specified1
normal vegetative cell shapemyo51ΔNull22
viable vegetative cell with normal cell morphology3092
penetrance FYPO_EXT:0000001myo51ΔNull
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in for3 formin For3 PMID:23051734
FYPO affected by mutation in myo51 myosin type V PMID:24798735
FYPO affected by mutation in rng8 contractile ring myosin V regulator Rng8 PMID:24798735
FYPO affected by mutation in rng9 contractile ring myosin V regulator Rng9 PMID:24798735
GO regulated by rng8 contractile ring myosin V regulator Rng8 PMID:24798735
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
129949292994786
229947302990810
329907702990178

UTRs

Region Coordinates Reference
five_prime_UTR2994929..2994815PMID:21511999
three_prime_UTR2990304..2990178PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00063 Pfam IPR001609 Myosin head, motor domain 69 737 5
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 777 795 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 801 820 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 754 773 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 850 869 3
PF01843 Pfam IPR018444 Dil domain 1298 1399 3
SM00242 SMART IPR001609 Myosin head, motor domain 59 750 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 870 892 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 774 796 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 847 869 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 799 821 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 751 773 3
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 848 877 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 777 804 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 753 781 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 800 825 5
PS51456 Prosite Profiles IPR001609 Myosin head, motor domain 65 749 5
PS51126 Prosite Profiles IPR002710 Dilute 1171 1417 3
PTHR13140 HMMPANTHER 8 1119 6
PTHR13140:SF289 HMMPANTHER 8 1119 1
1.20.5.190 Gene3D 777 818 2
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 150 180 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 797 844 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 40 800 294
Coil ncoils Rabaptin coiled-coil domain 967 988 971
PR00193 PRINTS IPR001609 Myosin head, motor domain 95 114 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 201 228 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 152 177 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 487 515 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 434 462 5

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 113.98 Da
Charge 53.00
Isoelectric point 9.29
Molecular weight 167.66 kDa
Number of residues 1471
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1131during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
965during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1208during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
786during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1223.96during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1073during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
987.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.39during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
rng9contractile ring myosin V regulator Rng9 Affinity Capture-WesternPMID:24798735
myo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
rng8contractile ring myosin V regulator Rng8 Affinity Capture-WesternPMID:24798735
myp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
rng2IQGAP Affinity Capture-RNAPMID:19805578
myo1myosin type I Affinity Capture-RNAPMID:19805578
myo51myosin type V Affinity Capture-WesternPMID:24798735
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
myo52myosin type V Affinity Capture-RNAPMID:19805578
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
ams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
pnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:18818364
myo2myosin II heavy chain Phenotypic EnhancementPMID:24798735
Synthetic Lethality
Phenotypic EnhancementPMID:23185032
ase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
lub1WD repeat protein Lub1 Negative GeneticPMID:18818364
cyp4cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp4 (predicted) Negative GeneticPMID:22681890
pyp1tyrosine phosphatase Pyp1 Positive GeneticPMID:22681890
ftp105Ubp5 interacting protein Ftp105 Negative GeneticPMID:18818364
mid1medial ring protein Mid1 Positive GeneticPMID:22681890
myo52myosin type V Phenotypic EnhancementPMID:23051734
mal3EB1 family Mal3 Phenotypic EnhancementPMID:23051734
ric1Ypt/Rab-specific guanyl-nucleotide exchange factor (GEF) subunit Ric1 Synthetic LethalityPMID:24798735
rlc1myosin II regulatory light chain Rlc1 Synthetic Growth DefectPMID:24798735
rad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
dao1D-amino acid oxidase Positive GeneticPMID:22681890
cyk3cytokinesis protein Cyk3 (predicted) Negative GeneticPMID:22681890
pit1serine/threonine protein kinase, meiotic Pit1 Positive GeneticPMID:22681890
SPBC30B4.06cmitochondrial GIDA family tRNA uridine 5-carboxymethylaminomethyl modification enzyme (predicted) Negative GeneticPMID:22681890
bob1prefoldin subunit 5 (predicted) Negative GeneticPMID:22681890
myp2myosin II heavy chain Myo3 Synthetic Growth DefectPMID:24798735
mrc1mediator of replication checkpoint 1 Negative GeneticPMID:18818364
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
ppm1leucine carboxyl methyltransferase Ppm1 (predicted) Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:22681890
ain1alpha-actinin Synthetic Growth DefectPMID:24798735
Phenotypic Enhancement
External References
Database Identifier Description
NBRP SPBC2D10.14c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC2D10.14c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC2D10.14c BioGRID Interaction Datasets
Expression Viewer SPBC2D10.14c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC2D10.14c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC2D10.14c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC2D10.14c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC2D10.14c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC2D10.14c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC2D10.14c Cell Cycle Data
GEO SPBC2D10.14c GEO profiles
PInt SPBC2D10.14c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC2D10.14c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC2D10.14c Fission yeast phenotypic data & analysis
SPD / RIKEN30/30H10Orfeome Localization Data
UniProtKB/SwissProtO74805Myosin-51
ModBaseO74805Database of comparative protein structure models
STRINGO74805Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596233myosin type V
RefSeq mRNANM_001022153972h- myosin type V (myo51), mRNA
European Nucleotide ArchiveCAA21172.1ENA Protein Mapping
UniParcUPI0000069F9DUniProt Archive

Literature for myo51

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015