myo51 (SPBC2D10.14c)


Gene Standard Namemyo51 Characterisation Statuspublished
Systematic IDSPBC2D10.14c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type V Product Size1471aa, 167.66 kDa
Genomic Location Chromosome II, 2994929-2990178 (4752nt); CDS:2994814-2990305 (4510nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0003779actin bindingIEAUniProtKB-KW:KW-0009GO_REF:000003738
GO:0005516calmodulin bindingIEAUniProtKB-KW:KW-0112GO_REF:000003710
GO:0000146microfilament motor activityIDAPMID:247987354
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0061572actin filament bundle organizationIMPPMID:2305173414
GO:0000915actomyosin contractile ring assemblyIMPPMID:1111269126
GO:0070649formin-nucleated actin cable assemblyIMPPMID:230517342
GO:1903117regulation of actin filament organization involved in cytokinetic actomyosin contractile ring assemblyIMPPMID:247987351
GO:0090337regulation of formin-nucleated actin cable assemblyIMPPMID:247987351
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005826actomyosin contractile ringIDAPMID:2479873531
during mitotic interphaseIDAPMID:11112691
GO:0032153cell division siteIDAPMID:23051734295
GO:0005829cytosolIDAPMID:168233722316
GO:0070648formin-nucleated actin cableIDAPMID:230517342
IDAPMID:24798735
GO:0016459myosin complexIEAUniProtKB-KW:KW-0518GO_REF:00000378
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationnot recorded (unrecorded)PMID:111126913760
Microscopymyo51ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopymyo51ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002032abnormal actin cable morphology during mitosisMicroscopymyo51ΔNullPMID:247987351
FYPO:0003444abnormal medial cortical node migrationMicroscopymyo51ΔNullPMID:247987351
FYPO:0000705abolished protein binding51
affecting rng9
affecting myo51
Co-immunoprecipitation experiment1-900 (901-1471)Not specifiedPMID:24798735
FYPO:0003442abolished protein localization to actin cable1
affecting rng8Microscopymyo51ΔNullPMID:24798735
affecting rng9Microscopymyo51ΔNullPMID:24798735
FYPO:0003440cell lysis during cytokinesis3
penetrance FYPO_EXT:0000003Microscopymyo51ΔNullPMID:24798735
FYPO:0003443decreased medial cortical node movement during contractile ring assemblyMicroscopymyo51ΔNullPMID:247987351
FYPO:0002441decreased rate of actin cable retrograde transportMicroscopymyo51ΔNullPMID:230517342
FYPO:0003339decreased rate of actomyosin contractile ring assemblyMicroscopy753-1471 (1-752)Not specifiedPMID:247987352
Microscopymyo51ΔNullPMID:24798735
FYPO:0001122elongated vegetative cellnot recorded (overexpression)PMID:11112691649
FYPO:0000118multiseptate91
penetrance FYPO_EXT:0000003Microscopymyo51ΔNullPMID:24798735
FYPO:0002487normal actin cable morphologyMicroscopymyo51ΔNullPMID:230517343
FYPO:0001294normal actin cortical patch localizationMicroscopymyo51ΔNullPMID:230517349
FYPO:0000703normal protein binding30
affecting rng9
affecting myo51
Co-immunoprecipitation experiment1-1294 (1293-1471)Not specifiedPMID:24798735
FYPO:0002559normal protein localization to actomyosin contractile ring14
affecting rng8Microscopymyo51ΔNullPMID:24798735
affecting rng9Microscopymyo51ΔNullPMID:24798735
FYPO:0001587normal protein localization to cell tip14
affecting myo52Microscopymyo51ΔNullPMID:23051734
affecting for3Microscopymyo51ΔNullPMID:23051734
FYPO:0002443normal rate of actin cable retrograde transportMicroscopymyo51-ΔTail (C-terminal truncation)Not specifiedPMID:230517341
FYPO:0001125normal vegetative cell shapeMicroscopymyo51ΔNullPMID:2305173421
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopymyo51ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in for3 formin For3 PMID:23051734
FYPO affected by mutation in myo51 myosin type V PMID:24798735
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
129949292994786
229947302990810
329907702990178

UTRs

Region Coordinates Reference
five_prime_UTR2994929..2994815PMID:21511999
three_prime_UTR2990304..2990178PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00063 Pfam IPR001609 Myosin head, motor domain 69 737 5
PF01843 Pfam IPR018444 Dil domain 1298 1399 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 777 795 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 850 869 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 754 773 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 801 820 3
SM00242 SMART IPR001609 Myosin head, motor domain 59 750 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 847 869 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 799 821 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 751 773 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 870 892 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 774 796 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 800 825 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 753 781 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 777 804 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 848 877 5
PS51126 Prosite Profiles IPR002710 Dilute 1171 1417 3
PTHR13140:SF289 HMMPANTHER 8 1119 1
PTHR13140 HMMPANTHER 8 1119 6
1.20.5.190 Gene3D 777 818 2
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 40 800 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 797 844 294
Coil ncoils Rabaptin coiled-coil domain 967 988 968
PR00193 PRINTS IPR001609 Myosin head, motor domain 152 177 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 201 228 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 95 114 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 487 515 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 434 462 5

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 113.98 Da
Charge 53.00
Isoelectric point 9.29
Molecular weight 167.66 kDa
Number of residues 1471
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1073during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
965during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
987.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
1223.96during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1208during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1131during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
786during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.39during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
pnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:18818364
myo2myosin II heavy chain Synthetic LethalityPMID:24798735
Phenotypic Enhancement
Phenotypic EnhancementPMID:23185032
ase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
lub1WD repeat protein Lub1 Negative GeneticPMID:18818364
cyp4cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp4 (predicted) Negative GeneticPMID:22681890
pyp1tyrosine phosphatase Pyp1 Positive GeneticPMID:22681890
ftp105Ubp5 interacting protein Ftp105 Negative GeneticPMID:18818364
mid1medial ring protein Mid1 Positive GeneticPMID:22681890
myo52myosin type V Phenotypic EnhancementPMID:23051734
mal3EB1 family Mal3 Phenotypic EnhancementPMID:23051734
ric1Ypt/Rab-specific guanyl-nucleotide exchange factor (GEF) subunit Ric1 Synthetic LethalityPMID:24798735
rlc1myosin II regulatory light chain Rlc1 Synthetic Growth DefectPMID:24798735
rad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
dao1D-amino acid oxidase Positive GeneticPMID:22681890
cyk3cytokinesis protein Cyk3 (predicted) Negative GeneticPMID:22681890
pit1serine/threonine protein kinase, meiotic Pit1 Positive GeneticPMID:22681890
SPBC30B4.06cmitochondrial GIDA family tRNA uridine 5-carboxymethylaminomethyl modification enzyme (predicted) Negative GeneticPMID:22681890
bob1prefoldin subunit 5 (predicted) Negative GeneticPMID:22681890
myp2myosin II heavy chain Myo3 Synthetic Growth DefectPMID:24798735
mrc1mediator of replication checkpoint 1 Negative GeneticPMID:18818364
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
ppm1leucine carboxyl methyltransferase Ppm1 (predicted) Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:22681890
ain1alpha-actinin Phenotypic EnhancementPMID:24798735
Synthetic Growth Defect
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rng9contractile ring myosin V regulator Rng9 Affinity Capture-WesternPMID:24798735
myo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
rng8contractile ring myosin V regulator Rng8 Affinity Capture-WesternPMID:24798735
myp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
rng2IQGAP Affinity Capture-RNAPMID:19805578
myo1myosin type I Affinity Capture-RNAPMID:19805578
myo51myosin type V Affinity Capture-WesternPMID:24798735
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
myo52myosin type V Affinity Capture-RNAPMID:19805578
External References
Database Identifier Description
NBRP SPBC2D10.14c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC2D10.14c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC2D10.14c BioGRID Interaction Datasets
Expression Viewer SPBC2D10.14c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC2D10.14c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC2D10.14c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC2D10.14c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC2D10.14c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC2D10.14c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC2D10.14c Cell Cycle Data
GEO SPBC2D10.14c GEO profiles
PInt SPBC2D10.14c Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2540560myosin type V
WikiGene2540560myosin type V
SPD / RIKEN30/30H10Orfeome Localization Data
UniProtKB/SwissProtO74805Myosin-51
ModBaseO74805Database of comparative protein structure models
STRINGO74805Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596233myosin type V
RefSeq mRNANM_001022153972h- myosin type V (myo51), mRNA
European Nucleotide ArchiveCAA21172ENA Protein Mapping
European Nucleotide ArchiveCAA21172.1ENA Protein Mapping
UniParcUPI0000069F9DUniProt Archive

Literature for myo51

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014