myo51 (SPBC2D10.14c)


Gene Standard Namemyo51 Characterisation Statuspublished
Systematic IDSPBC2D10.14c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type V Product Size1471aa, 167.66 kDa
Genomic Location Chromosome II, 2994929-2990178 (4752nt); CDS:2994814-2990305 (4510nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
calmodulin binding13
Annotation ExtensionEvidenceWith/FromReference
microfilament motor activity4
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin filament bundle organization14
Annotation ExtensionEvidenceWith/FromReference
formin-nucleated actin cable assembly2
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly28
Annotation ExtensionEvidenceWith/FromReference
protein localization to actin fusion focus2
Annotation ExtensionEvidenceWith/FromReference
regulation of actin filament organization involved in mitotic actomyosin contractile ring assembly1
Annotation ExtensionEvidenceWith/FromReference
regulation of formin-nucleated actin cable assembly1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin fusion focus13
Annotation ExtensionEvidenceWith/FromReference
actomyosin contractile ring39
Annotation ExtensionEvidenceWith/FromReference
cell division site313
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
formin-nucleated actin cable2
Annotation ExtensionEvidenceWith/FromReference
myosin complex8
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationmyo51Δ3837

Cell Phenotype

Term NameGenotypesCount
abnormal actin cable morphology during mitosismyo51Δ1
abnormal medial cortical node migrationmyo51Δ1
abnormal protein localization to shmoo tip2
affects localization of fus1myo51Δ
abolished protein binding124
affecting myo51 and rng91-900 (901-1471)
abolished protein localization to actin cable1
affects localization of rng8myo51Δ
affects localization of rng9myo51Δ
cell lysis during cytokinesismyo51Δ10
decreased medial cortical node movement during contractile ring assemblymyo51Δ1
decreased rate of actin cable retrograde transportmyo51Δ2
decreased rate of actomyosin contractile ring assemblymyo51Δ5
753-1471 (1-752)
elongated vegetative cellmyo51+ (wild type)791
multiseptate vegetative cellmyo51Δ97
narrow cell fusion site following conjugation with cellular fusionmyo51Δ1
normal actin cable morphologymyo51Δ4
normal actin cortical patch localizationmyo51Δ18
normal protein binding73
affecting myo51 and rng91-1294 (1293-1471)
normal protein localization to actomyosin contractile ring15
affecting rng8myo51Δ
affecting rng9myo51Δ
normal protein localization to cell tip24
affecting myo52myo51Δ
affecting for3myo51Δ
normal rate of actin cable retrograde transportmyo51-ΔTail (C-terminal truncation)1
normal rate of actomyosin contractile ring contractionmyo51Δ3
normal vegetative cell shapemyo51Δ31
viable vegetative cell with normal cell morphologymyo51Δ3100

FYPO Multi-allele Phenotypes

Cell Phenotype

Term NameGenotypes
abnormal endoplasmic reticulum localizationmyo52Δ, scs22Δ, for3Δ, scs2Δ, myo51Δ
abnormal protein localization to shmoo tip
affects localization of fus1myo52Δ, myo51Δ
abolished cell fusion during conjugationmyo52Δ, myo51Δ
stubby cellmyo52Δ, scs22Δ, for3Δ, scs2Δ, myo51Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in myo51 myosin type V
FYPO localization affected by mutation in for3 formin For3
FYPO localization affected by mutation in rng8 contractile ring myosin V regulator Rng8
FYPO localization affected by mutation in rng9 contractile ring myosin V regulator Rng9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2994929..2994786, 2994730..2990810, 2990770..2990178
Intron2994785..2994731, 2990809..2990771
5' UTR2994929..2994815PMID:21511999
3' UTR2990304..2990178PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 801 820 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 850 869 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 754 773 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 777 795 3
PF00063 Pfam IPR001609 Myosin head, motor domain 69 737 5
PF01843 Pfam IPR002710 Dilute domain 1299 1396 3
SM00242 SMART IPR001609 Myosin head, motor domain 59 750 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 870 892 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 799 821 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 751 773 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 847 869 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 774 796 3
PS51456 Prosite Profiles IPR001609 Myosin head, motor domain 65 749 5
PS51126 Prosite Profiles IPR002710 Dilute domain 1171 1417 3
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 753 781 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 848 877 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 777 804 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 800 825 5
PTHR13140:SF414 HMMPANTHER 1004 1031 1
PTHR13140:SF414 HMMPANTHER 12 943 1
PTHR13140 HMMPANTHER 1004 1031 8
PTHR13140 HMMPANTHER 12 943 8
1.20.5.190 Gene3D IQ motif, EF-hand binding site 777 818 2
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 150 180 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 797 844 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 40 800 294
Coil ncoils Predicted coiled-coil protein (DUF2205) 967 987 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 1459 1471 1057
PR00193 PRINTS IPR001609 Myosin head, motor domain 152 177 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 201 228 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 95 114 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 434 462 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 487 515 5

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 113.98 Da
Charge 53.00
Codon Adaptation Index 0.40
Isoelectric point 9.29
Molecular weight 167.66 kDa
Number of residues 1471
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for myo51 (SPBC2D10.14c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1131during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
965during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1208during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
786during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1073during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1223.96during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
987.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.39during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC2D10.14c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesrng2IQGAP Affinity Capture-RNAPMID:19805578
affinity capturesrng8contractile ring myosin V regulator Rng8 Affinity Capture-WesternPMID:24798735
affinity capturesmyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity capturesrng9contractile ring myosin V regulator Rng9 Affinity Capture-WesternPMID:24798735
affinity capturesmyo52myosin type V Affinity Capture-RNAPMID:19805578
affinity capturesmyo1myosin type I Affinity Capture-RNAPMID:19805578
affinity capturesmyo51myosin type V Affinity Capture-WesternPMID:24798735
affinity captured byrng3UCS-domain protein Rng3 Affinity Capture-RNAPMID:19098712
affinity captured byrng9contractile ring myosin V regulator Rng9 Affinity Capture-WesternPMID:24798735
affinity captured byrng8contractile ring myosin V regulator Rng8 Affinity Capture-WesternPMID:24798735
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC2D10.14c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withSPBC30B4.06cmitochondrial GIDA family tRNA uridine 5-carboxymethylaminomethyl modification enzyme (predicted) Negative GeneticPMID:22681890
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
negative genetic interaction withbob1prefoldin subunit 5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withelp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmrc1mediator of replication checkpoint 1 Negative GeneticPMID:18818364
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
negative genetic interaction withcyp4cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
negative genetic interaction withcyk3cytokinesis protein Cyk3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
negative genetic interaction withppm1leucine carboxyl methyltransferase, involved in regulation of autophagy Ppm1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withlub1WD repeat protein Lub1 Negative GeneticPMID:18818364
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:18818364
negative genetic interaction withftp105Ubp5 interacting protein Ftp105 Negative GeneticPMID:18818364
synthetic growth defect withmyp2myosin II heavy chain Myo3 Synthetic Growth DefectPMID:24798735
synthetic growth defect withain1alpha-actinin Synthetic Growth DefectPMID:24798735
synthetic growth defect withrlc1myosin II regulatory light chain Rlc1 Synthetic Growth DefectPMID:24798735
synthetic growth defect withmyo2myosin II heavy chain Synthetic Growth DefectPMID:24798735
positive genetic interaction withdao1D-amino acid oxidase Positive GeneticPMID:22681890
positive genetic interaction withmid1medial ring protein Mid1 Positive GeneticPMID:22681890
positive genetic interaction withpit1serine/threonine protein kinase, meiotic Pit1 Positive GeneticPMID:22681890
positive genetic interaction withpyp1tyrosine phosphatase Pyp1 Positive GeneticPMID:22681890
phenotype enhanced bymal3EB1 family Mal3 Phenotypic EnhancementPMID:23051734
phenotype enhanced bymyo52myosin type V Phenotypic EnhancementPMID:23051734
enhances phenotype ofmyo2myosin II heavy chain Phenotypic EnhancementPMID:23185032
External References
Database Identifier Description
NBRP SPBC2D10.14c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC2D10.14c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC2D10.14c BioGRID Interaction Datasets
Expression Viewer SPBC2D10.14c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC2D10.14c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC2D10.14c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC2D10.14c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC2D10.14c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC2D10.14c Transcriptome Viewer (Bähler Lab)
GEO SPBC2D10.14c GEO profiles
PInt SPBC2D10.14c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC2D10.14c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC2D10.14c Fission yeast phenotypic data & analysis
Cyclebase SPBC2D10.14c.1 Cell Cycle Data
SPD / RIKEN30/30H10Orfeome Localization Data
UniProtKB/SwissProtO74805Myosin-51
ModBaseO74805Database of comparative protein structure models
STRINGO74805Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596233myosin type V
RefSeq mRNANM_001022153972h- myosin type V (myo51), mRNA

Literature for myo51

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016