pmr1 (SPBC31E1.02c)


Gene Standard Namepmr1 Characterisation Statuspublished
Systematic IDSPBC31E1.02c Feature Typeprotein coding
Synonyms Name Description
ProductP-type ATPase, calcium transporting Pmr1 Product Size899aa, 98.36 kDa
Genomic Location Chromosome II, 243731-239838 (3894nt); CDS:243069-240370 (2700nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
calcium ion binding39
Annotation ExtensionEvidenceWith/FromReference
calcium-transporting ATPase activity5
Annotation ExtensionEvidenceWith/FromReference
manganese ion binding9
Annotation ExtensionEvidenceWith/FromReference
manganese-transporting ATPase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
calcium ion transmembrane transport15
Annotation ExtensionEvidenceWith/FromReference
Golgi calcium ion export2
Annotation ExtensionEvidenceWith/FromReference
manganese ion transmembrane transport4
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum595
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum membrane201
Annotation ExtensionEvidenceWith/FromReference
integral component of endoplasmic reticulum membrane68
Annotation ExtensionEvidenceWith/FromReference
plasma membrane254
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at high temperaturecps5-138 (400->opal)164
normal growth on glucose carbon sourcepmr1Δ263
normal growth on sorbitolcps5-138 (400->opal)20
normal vegetative cell population growth ratepmr1Δ79
sensitive to 5-fluorouracilpmr1Δ273
sensitive to cadmiumpmr1Δ249
sensitive to calcium starvationcps5-138 (400->opal)3
pmr1Δ
sensitive to cell wall-degrading enzymespmr1Δ46
cps5-138 (400->opal)
sensitive to cyclosporin Acps5-138 (400->opal)7
sensitive to hydrogen peroxidepmr1Δ153
sensitive to hydroxyureapmr1Δ547
sensitive to low osmolaritycps5-138 (400->opal)2
sensitive to salt stresspmr1Δ98
sensitive to UV during vegetative growthpmr1Δ148
viable vegetative cell populationpmr1Δ3831
pmr1+

Cell Phenotype

Term NameGenotypesCount
abnormal cell separation after cytokinesis resulting in septated cellcps5-138 (400->opal)20
decreased 1,3-beta-D-glucan synthase activitypmr1Δ6
cps5-138 (400->opal)
decreased cell wall galactomannan levelpmr1Δ5
cps5-138 (400->opal)
decreased protein glycosylationcps5-138 (400->opal)8
pmr1Δ
dispersed actin cortical patch localizationcps5-138 (400->opal)32
galactomannan absent from cell wallcps5-138 (400->opal)1
pmr1Δ
increased 1,3-beta-D-glucan synthase activitypmr1Δ5
cps5-138 (400->opal)
increased cell wall alpha-glucan level during cellular response to calcium starvationcps5-138 (400->opal)1
pmr1Δ
increased septum thicknesscps5-138 (400->opal)26
lemon-shaped cellcps5-138 (400->opal)12
normal DNA contentpmr1Δ23
viable vegetative cell with normal cell morphologypmr1+3099
viable vegetative cell, abnormal cell shape, normal cell sizepmr1Δ215

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
normal growth on calcium ioncta5Δ, pmc1Δ, pmr1+
normal vegetative cell population growth ratecta5Δ, pmr1Δ
slow vegetative cell population growthSPCC1672.11cΔ, pmr1Δ
Target Of
OntologyRelationshipGeneProduct
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons243731..239838
5' UTR243731..243070PMID:21511999
3' UTR240369..239838PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00122 Pfam IPR008250 P-type ATPase, A domain 87 318 12
PF00689 Pfam IPR006068 Cation-transporting P-type ATPase, C-terminal 710 880 3
PF00690 Pfam IPR004014 Cation-transporting P-type ATPase, N-terminal 7 75 6
PF00702 Pfam 323 639 6
SM00831 SMART IPR004014 Cation-transporting P-type ATPase, N-terminal 4 80 5
TMhelix TMHMM 78 100 960
TMhelix TMHMM 248 265 960
TMhelix TMHMM 280 302 960
TMhelix TMHMM 686 708 960
TMhelix TMHMM 757 779 960
TMhelix TMHMM 826 845 960
TMhelix TMHMM 855 874 960
PS00154 Prosite Patterns IPR018303 P-type ATPase, phosphorylation site 329 335 14
PTHR24093 HMMPANTHER 2 889 9
PTHR24093:SF217 HMMPANTHER IPR006413 P-type ATPase, subfamily IIA, PMR1-type 2 889 1
1.20.1110.10 Gene3D IPR023298 P-type ATPase, transmembrane domain 59 115 5
1.20.1110.10 Gene3D IPR023298 P-type ATPase, transmembrane domain 158 507 5
1.20.1110.10 Gene3D IPR023298 P-type ATPase, transmembrane domain 594 884 5
0049471 SuperFamily 117 223 14
0049473 SuperFamily 6 118 14
0049473 SuperFamily 228 321 14
0049473 SuperFamily 675 884 14
SSF56784 SuperFamily IPR023214 HAD-like domain 325 345 41
SSF56784 SuperFamily IPR023214 HAD-like domain 476 728 41
SSF81660 SuperFamily IPR023299 P-type ATPase, cytoplasmic domain N 333 528 11
PR00119 PRINTS IPR001757 P-type ATPase 165 179 14
PR00119 PRINTS IPR001757 P-type ATPase 327 341 14
PR00119 PRINTS IPR001757 P-type ATPase 516 527 14
PR00119 PRINTS IPR001757 P-type ATPase 538 548 14
PR00119 PRINTS IPR001757 P-type ATPase 625 644 14
PR00119 PRINTS IPR001757 P-type ATPase 649 661 14
PR00120 PRINTS IPR001757 P-type ATPase 486 504 4
PR00120 PRINTS IPR001757 P-type ATPase 597 613 4
PR00120 PRINTS IPR001757 P-type ATPase 625 641 4
PR00120 PRINTS IPR001757 P-type ATPase 657 682 4
TIGR01494 tigrfam IPR001757 P-type ATPase 87 210 14
TIGR01494 tigrfam IPR001757 P-type ATPase 222 348 14
TIGR01494 tigrfam IPR001757 P-type ATPase 596 710 14
TIGR01522 tigrfam IPR006413 P-type ATPase, subfamily IIA, PMR1-type 2 885 1

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 109.41 Da
Charge -3.50
Codon Adaptation Index 0.44
Isoelectric point 6.04
Molecular weight 98.36 kDa
Number of residues 899
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS892 1675
present during mitotic M phase, cellular response to thiabendazoleS892
Annotation ExtensionEvidenceResidueReference
experimental evidence S892 PMID:24763107
present during cellular response to thiabendazole IDA S892 PMID:18257517
present during mitotic M phase experimental evidence S892 PMID:21712547
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK343 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K343 PMID:26412298
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pmr1 (SPBC31E1.02c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
12667during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
12676during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
10921during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
12143during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
12141during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3414.34during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3765.91during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
Hailey-Hailey disease1
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
conserved in eukaryotes only2504
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPBC31E1.02c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
enhances phenotype ofbgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:15470240
overexpression rescuespmc1vacuolar calcium transporting P-type ATPase P2 type, Pmc1 Dosage RescuePMID:15470240
overexpression rescuescta5Ca2+/Mn2+ transporting P-type ATPase P5 type Cta5 Dosage RescuePMID:19168988
overexpression rescuesmed14mediator complex subunit Med14 Dosage RescuePMID:19168988
synthetic lethal withpmc1vacuolar calcium transporting P-type ATPase P2 type, Pmc1 Synthetic LethalityPMID:15470240
rescued by overexpression ofcta5Ca2+/Mn2+ transporting P-type ATPase P5 type Cta5 Dosage RescuePMID:19168988
rescued by overexpression ofpdt1Nramp family manganese ion transmembrane transporter Dosage RescuePMID:14723709
negative genetic interaction withmyp2myosin II heavy chain Myo3 Negative GeneticPMID:22681890
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
negative genetic interaction withprp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
negative genetic interaction withste20Rictor homolog, Ste20 Negative GeneticPMID:22681890
negative genetic interaction withpck1protein kinase C (PKC)-like Pck1 Negative GeneticPMID:22681890
negative genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
negative genetic interaction withppa2serine/threonine protein phosphatase Ppa2 Negative GeneticPMID:22681890
negative genetic interaction withSPAC732.02cfructose-2,6-bisphosphate 2-phosphatase activity (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction witheif21translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Negative GeneticPMID:22681890
negative genetic interaction withset1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
negative genetic interaction withpuf1pumilio family RNA-binding protein Puf1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
negative genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
negative genetic interaction withspa2cell polarity protein Spa2 Negative GeneticPMID:22681890
negative genetic interaction withint6eIF3e subunit Int6 Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC23A1.02cphosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withefc25Ras1 guanyl-nucleotide exchange factor Efc25 Negative GeneticPMID:22681890
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
negative genetic interaction withusp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
synthetic growth defect withryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
synthetic growth defect withalg6glucosyltransferase Alg6 Synthetic Growth DefectPMID:18931302
synthetic growth defect withnse5Smc5-6 complex non-SMC subunit Nse5 Synthetic Growth DefectPMID:18931302
synthetic growth defect withSPCC1672.11cP-type ATPase P5 type (predicted) Synthetic Growth DefectPMID:19168988
synthetic growth defect withyam8stretch-activated calcium ion channel Yam8 Synthetic Growth DefectPMID:18931302
synthetic growth defect withgls2glucosidase II alpha subunit Gls2 Synthetic Growth DefectPMID:18931302
synthetic growth defect withvps17retromer complex subunit Vps17 Synthetic Growth DefectPMID:18931302
synthetic growth defect withSPAC24H6.13DUF221 family protein implicated in Golgi to plasma membrane transport (predicted) Synthetic Growth DefectPMID:18931302
synthetic growth defect withcta5Ca2+/Mn2+ transporting P-type ATPase P5 type Cta5 Synthetic Growth DefectPMID:19168988
positive genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withelp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Positive GeneticPMID:22681890
phenotype enhanced bypdt1Nramp family manganese ion transmembrane transporter Phenotypic EnhancementPMID:14723709
External References
Database Identifier Description
NBRP SPBC31E1.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC31E1.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC31E1.02c BioGRID Interaction Datasets
Expression Viewer SPBC31E1.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC31E1.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC31E1.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC31E1.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC31E1.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC31E1.02c Transcriptome Viewer (Bähler Lab)
GEO SPBC31E1.02c GEO profiles
PInt SPBC31E1.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC31E1.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC31E1.02c Fission yeast phenotypic data & analysis
Cyclebase SPBC31E1.02c.1 Cell Cycle Data
IntEnz3.6.3.8Integrated relational Enzyme database
Rhea3.6.3.8Annotated reactions database
SPD / RIKEN33/33H08Orfeome Localization Data
UniProtKB/SwissProtO59868Calcium-transporting ATPase 1
ModBaseO59868Database of comparative protein structure models
STRINGO59868Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595098P-type ATPase, calcium transporting Pmr1
RefSeq mRNANM_001021005972h- P-type ATPase, calcium transporting Pmr1 (pmr1), mRNA
European Nucleotide ArchiveAC004698ENA EMBL mapping
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveAAC16669ENA Protein Mapping
European Nucleotide ArchiveCAB39136ENA Protein Mapping
UniParcUPI000006C86CUniProt Archive

Literature for pmr1

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016