pmr1 (SPBC31E1.02c)


Gene Standard Namepmr1 Characterisation Statuspublished
Systematic IDSPBC31E1.02c Feature Typeprotein coding
Synonymscps5, pgak2 Name Description
ProductP-type ATPase, calcium transporting Pmr1 Product Size899aa, 98.36 kDa
Genomic Location Chromosome II, 243731-239838 (3894nt); CDS:243069-240370 (2700nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0005509calcium ion bindingISOSGD:S000003135GO_REF:000002452
GO:0005388calcium-transporting ATPase activityISOSGD:S000003135GO_REF:00000246
GO:0030145manganese ion bindingICGO:0015410GO_REF:00000019
GO:0015410manganese-transporting ATPase activityTASPMID:147237091
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0070588calcium ion transmembrane transportISOSGD:S000003135GO_REF:000002412
IGIpdt1PMID:14723709
GO:0071421manganese ion transmembrane transportIMPPMID:147237094
GO:0050850positive regulation of calcium-mediated signalingIMPPMID:169289596
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0005789endoplasmic reticulum membraneIDAPMID:15470240206
GO:0030176integral component of endoplasmic reticulum membraneICGO:0005388GO_REF:000000167
GO:0005886plasma membraneIDAPMID:15470240242
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001164normal growth on glucose carbon sourceCell growth assaypmr1ΔNullPECO:0000102, PECO:0000137PMID:15470240145
FYPO:0000961normal growth on sorbitolCell growth assaycps5-138 (400->opal)Not specifiedPECO:0000004PMID:1547024010
FYPO:0001420normal vegetative cell population growth rateCell growth assaypmr1ΔNullPECO:0000005, PECO:0000080PMID:1916898838
FYPO:0000268sensitive to UV during vegetative growthCell growth assaypmr1ΔNullPECO:0000137, PECO:0000005PMID:23173672117
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000002cell growth assaypmr1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0001201sensitive to calcium starvationCell growth assaycps5-138 (400->opal)Not specifiedPECO:0000126PMID:154702403
Cell growth assaypmr1ΔNullPECO:0000150, PECO:0000126PMID:15470240
FYPO:0001190sensitive to cell wall-degrading enzymesCell growth assaypmr1ΔNullPECO:0000005, PECO:0000137PMID:1547024039
Cell growth assaycps5-138 (400->opal)Not specifiedPECO:0000137, PECO:0000005PMID:15470240
FYPO:0000105sensitive to cyclosporin AMicroscopycps5-138 (400->opal)Not specifiedPECO:0000137PMID:154702402
FYPO:0000082sensitive to heatCell growth assaycps5-138 (400->opal)Not specifiedPECO:0000126, PECO:0000004PMID:15470240138
FYPO:0000087sensitive to hydrogen peroxide121
expressivity FYPO_EXT:0000003cell growth assaypmr1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000088sensitive to hydroxyureaCell growth assaypmr1ΔNullPECO:0000137, PECO:0000005PMID:23173672506
FYPO:0002077sensitive to low osmolarityCell growth assaycps5-138 (400->opal)Not specifiedPECO:0000004PMID:154702402
FYPO:0000271sensitive to salt stress82
expressivity FYPO_EXT:0000003cell growth assaypmr1ΔNullPECO:0000207, PECO:0000137, PECO:0000102, PECO:0000005PMID:18684775
FYPO:0002060viable vegetative cell populationCell growth assaypmr1+ (wild type)OverexpressionPMID:154702403760
Microscopypmr1ΔNullPMID:20473289
Microscopypmr1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001972abnormal cytokinetic cell separation resulting in septated cellFlow cytometry datacps5-138 (400->opal)Not specifiedPECO:0000126, PECO:0000150PMID:154702404
FYPO:0002159decreased 1,3-beta-D-glucan synthase activityEnzyme assay datacps5-138 (400->opal)Not specifiedPECO:0000137, PECO:0000005PMID:154702404
Enzyme assay datapmr1ΔNullPECO:0000005, PECO:0000137, PECO:0000025PMID:15470240
Enzyme assay datacps5-138 (400->opal)Not specifiedPECO:0000025, PECO:0000137, PECO:0000004PMID:15470240
Enzyme assay datapmr1ΔNullPECO:0000137, PECO:0000004, PECO:0000025PMID:15470240
Enzyme assay datapmr1ΔNullPECO:0000005, PECO:0000137PMID:15470240
Enzyme assay datacps5-138 (400->opal)Not specifiedPECO:0000137, PECO:0000025, PECO:0000005PMID:15470240
FYPO:0002265decreased cell wall galactomannan levelSubstance quantification evidencecps5-138 (400->opal)Not specifiedPECO:0000126PMID:154702403
Substance quantification evidencepmr1ΔNullPECO:0000126PMID:15470240
FYPO:0001911decreased protein glycosylationGel electrophoresis evidencecps5-138 (400->opal)Not specifiedPMID:154702407
Gel electrophoresis evidencepmr1ΔNullPMID:15470240
FYPO:0002021dispersed actin cortical patch localizationMicroscopycps5-138 (400->opal)Not specifiedPECO:0000150, PECO:0000126PMID:1547024019
expressivity FYPO_EXT:0000003Microscopycps5-138 (400->opal)Not specifiedPECO:0000137, PECO:0000005PMID:15470240
Microscopycps5-138 (400->opal)Not specifiedPECO:0000137, PECO:0000004PMID:15470240
FYPO:0003133galactomannan absent from cell wallSubstance quantification evidencepmr1ΔNullPECO:0000126, PECO:0000150PMID:154702401
Substance quantification evidencecps5-138 (400->opal)Not specifiedPECO:0000150, PECO:0000126PMID:15470240
FYPO:0001968increased 1,3-beta-D-glucan synthase activityEnzyme assay datacps5-138 (400->opal)Not specifiedPECO:0000004, PECO:0000137PMID:154702405
Enzyme assay datapmr1ΔNullPECO:0000004, PECO:0000137PMID:15470240
FYPO:0003134increased cell wall alpha-glucan level during cellular response to calcium starvationSubstance quantification evidencepmr1ΔNullPECO:0000126, PECO:0000150PMID:154702401
Substance quantification evidencecps5-138 (400->opal)Not specifiedPECO:0000150, PECO:0000126PMID:15470240
FYPO:0001406increased septum thicknessMicroscopycps5-138 (400->opal)Not specifiedPECO:0000004, PECO:0000137PMID:1547024012
Microscopycps5-138 (400->opal)Not specifiedPECO:0000126, PECO:0000005PMID:15470240
Microscopycps5-138 (400->opal)Not specifiedPECO:0000137, PECO:0000004PMID:15470240
FYPO:0000224lemon-shaped cellMicroscopycps5-138 (400->opal)Not specifiedPECO:0000137, PECO:0000005PMID:154702409
Microscopycps5-138 (400->opal)Not specifiedPECO:0000126, PECO:0000005PMID:15470240
FYPO:0001383normal DNA contentFlow cytometry datapmr1ΔNullPECO:0000137, PECO:0000005PMID:2317367219
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopypmr1+ (wild type)OverexpressionPMID:154702403086
FYPO:0001510viable vegetative cell, abnormal cell shape, normal cell size215
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopypmr1ΔNullPECO:0000137, PECO:0000005PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1243731239838

UTRs

Region Coordinates Reference
five_prime_UTR243731..243070PMID:21511999
three_prime_UTR240369..239838PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00702 Pfam IPR023214 HAD-like domain 323 639 15
PF00122 Pfam IPR008250 P-type ATPase, A domain 86 319 14
PF00689 Pfam IPR006068 Cation-transporting P-type ATPase, C-terminal 710 880 3
PF00690 Pfam IPR004014 Cation-transporting P-type ATPase, N-terminal 7 75 6
SM00831 SMART IPR004014 Cation-transporting P-type ATPase, N-terminal 4 80 5
TMhelix TMHMM 280 302 959
TMhelix TMHMM 855 874 959
TMhelix TMHMM 826 845 959
TMhelix TMHMM 248 265 959
TMhelix TMHMM 757 779 959
TMhelix TMHMM 78 100 959
TMhelix TMHMM 686 708 959
PS00154 Prosite Patterns IPR018303 P-type ATPase, phosphorylation site 329 335 14
PTHR24093:SF217 HMMPANTHER 1 892 1
PTHR24093 HMMPANTHER 1 892 9
1.20.1110.10 Gene3D IPR023298 P-type ATPase, transmembrane domain 594 884 5
1.20.1110.10 Gene3D IPR023298 P-type ATPase, transmembrane domain 59 115 5
1.20.1110.10 Gene3D IPR023298 P-type ATPase, transmembrane domain 158 507 5
0049473 SuperFamily 675 884 14
0049473 SuperFamily 228 321 14
0049473 SuperFamily 6 118 14
SSF56784 SuperFamily IPR023214 HAD-like domain 476 728 41
SSF56784 SuperFamily IPR023214 HAD-like domain 325 345 41
SSF81660 SuperFamily IPR023299 P-type ATPase, cytoplasmic domain N 333 528 11
0049471 SuperFamily 117 223 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 165 179 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 538 548 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 625 644 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 649 661 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 327 341 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 516 527 14
PR00120 PRINTS IPR001757 Cation-transporting P-type ATPase 597 613 4
PR00120 PRINTS IPR001757 Cation-transporting P-type ATPase 657 682 4
PR00120 PRINTS IPR001757 Cation-transporting P-type ATPase 625 641 4
PR00120 PRINTS IPR001757 Cation-transporting P-type ATPase 486 504 4
TIGR01494 tigrfam IPR001757 Cation-transporting P-type ATPase 87 210 14
TIGR01494 tigrfam IPR001757 Cation-transporting P-type ATPase 596 710 14
TIGR01494 tigrfam IPR001757 Cation-transporting P-type ATPase 222 348 14
TIGR01522 tigrfam IPR006413 Calcium-transporting P-type ATPase, subfamily IIA, PMR1-type 2 885 1

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 109.41 Da
Charge -3.50
Isoelectric point 6.04
Molecular weight 98.36 kDa
Number of residues 899
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS892PMID:247631071663
present during mitotic M phaseS892PMID:21712547
present during cellular response to thiabendazoleIDAS892PMID:18257517
MOD:00696phosphorylated residueNASPMID:182575171915
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
3414.34during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
12141during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
10921during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
12676during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
12667during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
12143during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3765.91during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
4.9during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
Hailey-Hailey disease1
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pmc1mediator complex subunit Pmc1 Dosage RescuePMID:19168988
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
vps17retromer complex subunit Vps17 Synthetic Growth DefectPMID:18931302
set1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
efc25Ras1 guanyl-nucleotide exchange factor Efc25 Negative GeneticPMID:22681890
SPAC23A1.02cphosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
SPAC732.02cfructose-2,6-bisphosphate 2-phosphatase activity (predicted) Negative GeneticPMID:22681890
gls2glucosidase II alpha subunit Gls2 Synthetic Growth DefectPMID:18931302
cta5Ca2+/Mn2+ transporting P-type ATPase P5 type Cta5 Synthetic Growth DefectPMID:19168988
Dosage Rescue
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
myp2myosin II heavy chain Myo3 Negative GeneticPMID:22681890
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
ste20Rictor homolog, Ste20 Negative GeneticPMID:22681890
SPBC4B4.04translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
pck1protein kinase C (PKC)-like Pck1 Negative GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
SPCC1672.11cP-type ATPase P5 type (predicted) Synthetic Growth DefectPMID:19168988
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
yam8calcium channel regulatory subunit Yam8 Synthetic Growth DefectPMID:18931302
ryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
gma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
int6eIF3e subunit Int6 Negative GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
nse5Smc5-6 complex non-SMC subunit Nse5 Synthetic Growth DefectPMID:18931302
usp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:15470240
pdt1Nramp family manganese ion transporter Phenotypic EnhancementPMID:14723709
Dosage Rescue
SPAC24H6.13DUF221 family protein implicated in Golgi to plasma membrane transport (predicted) Synthetic Growth DefectPMID:18931302
spa2GTPase activating protein Spa2 (predicted) Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
SPAPB2B4.04cvacuolar calcium transporting P-type ATPase P2 type, Pmc1 Synthetic LethalityPMID:15470240
Dosage Rescue
vps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
ubp2ubiquitin C-terminal hydrolase Ubp2 Positive GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
alg6glucosyltransferase Alg6 Synthetic Growth DefectPMID:18931302
ppa2serine/threonine protein phosphatase Ppa2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC31E1.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC31E1.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC31E1.02c BioGRID Interaction Datasets
Expression Viewer SPBC31E1.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC31E1.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC31E1.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC31E1.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC31E1.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC31E1.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC31E1.02c Cell Cycle Data
GEO SPBC31E1.02c GEO profiles
PInt SPBC31E1.02c Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz3.6.3.8Integrated relational Enzyme database
Rhea3.6.3.8Annotated reactions database
WikiGene2540383P-type ATPase, calcium transporting Pmr1
EntrezGene2540383P-type ATPase, calcium transporting Pmr1
SPD / RIKEN33/33H08Orfeome Localization Data
UniProtKB/SwissProtO59868Calcium-transporting ATPase 1
ModBaseO59868Database of comparative protein structure models
STRINGO59868Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595098P-type ATPase, calcium transporting Pmr1
RefSeq mRNANM_001021005972h- P-type ATPase, calcium transporting Pmr1 (pmr1), mRNA
European Nucleotide ArchiveAAC16669ENA Protein Mapping
European Nucleotide ArchiveAAC16669.1ENA Protein Mapping
European Nucleotide ArchiveCAB39136ENA Protein Mapping
European Nucleotide ArchiveCAB39136.1ENA Protein Mapping
UniParcUPI000006C86CUniProt Archive

Literature for pmr1

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014