cwf4 (SPBC31F10.11c)


Gene Standard Namecwf4 Characterisation Statuspublished
Systematic IDSPBC31F10.11c Feature Typeprotein coding
Synonymssyf3 Name DescriptionComplexed With cdc Five
Productcomplexed with Cdc5 protein Cwf4 Product Size674aa, 80.84 kDa
Genomic Location Chromosome II, 3773191-3770856 (2336nt); CDS:3773057-3771033 (2025nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
mRNA cis splicing, via spliceosome118
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Prp19 complex16
Annotation ExtensionEvidenceWith/FromReference
spliceosomal complex108
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationcwf4ΔNull1428

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclecwf4ΔNull833
inviable after spore germination with elongated germ tubecwf4ΔNull163
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in iss10 Mmi1-driven selective elimination factor Iss10 PMID:24713849
FYPO affected by mutation in mtl1 TRAMP complex ATP-dependent RNA helicase (predicted) PMID:24713849
FYPO affected by mutation in rmn1 RNA-binding protein PMID:24713849
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3773191..3770856
mRNA3773191..3770856
5' UTR3773191..3773058PMID:21511999
CDS3773057..3771033
3' UTR3771032..3770856PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02184 Pfam IPR003107 HAT (Half-A-TPR) repeat 519 547 1
PF02184 Pfam IPR003107 HAT (Half-A-TPR) repeat 185 216 1
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 333 367 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 482 516 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 50 82 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 415 446 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 218 253 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 255 289 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 448 480 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 377 413 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 185 216 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 84 116 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 299 331 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 518 549 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 152 183 8
SM00386 SMART IPR003107 HAT (Half-A-TPR) repeat 118 150 8
PS50293 Prosite Profiles IPR013026 Tetratricopeptide repeat-containing domain 241 274 27
PS50293 Prosite Profiles IPR013026 Tetratricopeptide repeat-containing domain 378 434 27
PS50293 Prosite Profiles IPR013026 Tetratricopeptide repeat-containing domain 70 171 27
PTHR11246:SF3 HMMPANTHER 2 671 1
PTHR11246 HMMPANTHER 2 671 3
1.25.40.10 Gene3D IPR011990 Tetratricopeptide-like helical domain 308 388 62
1.25.40.10 Gene3D IPR011990 Tetratricopeptide-like helical domain 252 275 62
1.25.40.10 Gene3D IPR011990 Tetratricopeptide-like helical domain 389 495 62
1.25.40.10 Gene3D IPR011990 Tetratricopeptide-like helical domain 73 202 62
SSF48452 SuperFamily 505 616 50
SSF48452 SuperFamily 60 501 50

View domain organization at Pfam

Protein Properties

Ave. residue weight 119.94 Da
Charge 4.00
Isoelectric point 7.12
Molecular weight 80.84 kDa
Number of residues 674
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3136during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3661during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2657during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3485during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3584.76during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3145during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1473.34during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.4during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.98during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withcdc11SIN component scaffold protein, centriolin ortholog Cdc11 Two-hybridPMID:23695164
co-purifies withcdc5splicing factor, Prp19 complex subunit Cdc5 Co-purificationPMID:24014766
affinity captured bycdc5splicing factor, Prp19 complex subunit Cdc5 Affinity Capture-MSPMID:15755920
affinity capturescdc5splicing factor, Prp19 complex subunit Cdc5 Affinity Capture-WesternPMID:10409726
affinity captured bycdc5splicing factor, Prp19 complex subunit Cdc5 Affinity Capture-MSPMID:10409726
affinity captured bycwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:24442611
affinity captured bycwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-WesternPMID:24442611
co-purifies withcwf10U5 snRNP GTPase subunit Cwf10 Co-purificationPMID:24014766
affinity captured bycwf14G10 protein Affinity Capture-MSPMID:24874881
affinity captured bycwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
affinity captured bydre4splicing associated factor Dre4 Affinity Capture-MSPMID:21386897
affinity captured byepe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
affinity captured bylea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:24442611
affinity captured bylea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-WesternPMID:24442611
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured bynab2poly(A) binding protein Nab2 (predicted) Affinity Capture-MSPMID:24081329
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured byprp17splicing factor Prp17 Affinity Capture-MSPMID:21386897
affinity captured byprp19ubiquitin-protein ligase E4 Prp19 Affinity Capture-MSPMID:21386897
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
affinity captured byred5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
affinity captured byrmn1RNA-binding protein Affinity Capture-MSPMID:24713849
affinity captured bysaf2splicing associated factor Saf2 Affinity Capture-MSPMID:21386897
affinity captured bysaf3splicing associated factor Saf3 Affinity Capture-MSPMID:21386897
affinity captured bysaf5splicing factor Saf5 Affinity Capture-MSPMID:25274039
affinity captured bysde2silencing defective protein Sde2 Affinity Capture-MSPMID:25274039
affinity captured bytls1splicing factor Tls1 Affinity Capture-MSPMID:25245948
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withmto1MT organizer Mto1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC31F10.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC31F10.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC31F10.11c BioGRID Interaction Datasets
Expression Viewer SPBC31F10.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC31F10.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC31F10.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC31F10.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC31F10.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC31F10.11c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC31F10.11c Cell Cycle Data
GEO SPBC31F10.11c GEO profiles
PInt SPBC31F10.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC31F10.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC31F10.11c Fission yeast phenotypic data & analysis
SPD / RIKEN46/46C09Orfeome Localization Data
UniProtKB/SwissProtP87312Pre-mRNA-splicing factor cwf4
ModBaseP87312Database of comparative protein structure models
STRINGP87312Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596573complexed with Cdc5 protein Cwf4
RefSeq mRNANM_001022494972h- complexed with Cdc5 protein Cwf4 (cwf4), mRNA
European Nucleotide ArchiveAAF67752.1ENA Protein Mapping
European Nucleotide ArchiveCAB10088.1ENA Protein Mapping
UniParcUPI0000128734UniProt Archive

Literature for cwf4

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015