rec14 (SPBC32F12.02)


Gene Standard Namerec14 Characterisation Statuspublished
Systematic IDSPBC32F12.02 Feature Typeprotein coding
Synonyms Name Description
ProductSki complex subunit Rec14 Product Size302aa, 32.94 kDa
Genomic Location Chromosome II, 2786858-2788406 (1549nt); CDS:2786978-2787939 (962nt)

Ensembl Gene Location
GO Biological Process
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
Ski complex3
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased sporulation frequencyrec14Δ103
slow vegetative cell population growthrec14-120357
viable vegetative cell populationrec14Δ3840

Cell Phenotype

Term NameGenotypesCount
abnormal linear element morphologyrec14Δ6
decreased meiotic recombinationrec14-12048
normal mitotic recombinationrec14-1207
viable vegetative cell with normal cell morphologyrec14Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2786858..2787266, 2787320..2788406
Intron2787267..2787319
mRNA2786858..2788406
5' UTR2786858..2786977PMID:21511999
CDS2786978..2787266, 2787320..2787939
3' UTR2787940..2788406PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08662 Pfam IPR013979 Translation initiation factor, beta propellor-like domain 167 286 5
SM00320 SMART IPR001680 WD40 repeat 218 257 112
SM00320 SMART IPR001680 WD40 repeat 134 173 112
SM00320 SMART IPR001680 WD40 repeat 176 215 112
SM00320 SMART IPR001680 WD40 repeat 92 131 112
SM00320 SMART IPR001680 WD40 repeat 47 87 112
SM00320 SMART IPR001680 WD40 repeat 260 297 112
SM00320 SMART IPR001680 WD40 repeat 3 42 112
PS50082 Prosite Profiles IPR001680 WD40 repeat 183 224 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 225 266 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 99 131 100
PS50294 Prosite Profiles IPR017986 WD40-repeat-containing domain 12 302 104
PTHR22847 HMMPANTHER 29 294 13
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 13 298 145
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 29 298 135

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 109.06 Da
Charge -10.50
Codon Adaptation Index 0.42
Isoelectric point 4.80
Molecular weight 32.94 kDa
Number of residues 302
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0051321Northern assay evidencePMID:9258671

Quantitative Gene Expression

View graphical display of gene expression data for rec14 (SPBC32F12.02)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
16332during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14633during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14374during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14677during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14594during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4666.24during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2111.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.43during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC32F12.02 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withrec12meiotic recombination endonuclease Rec12 Two-hybridPMID:22841486
binds DNA-binding domain construct withmde2Mde2 protein Two-hybridPMID:20364342
forms complex withmde2Mde2 protein Reconstituted ComplexPMID:22841486
binds activation domain construct withmde2Mde2 protein Two-hybridPMID:22841486
binds activation domain construct withski3Ski complex TPR repeat subunit Ski3 (predicted) Two-hybridPMID:26771498
affinity captured bymde2Mde2 protein Affinity Capture-WesternPMID:22841486
affinity captured byrec12meiotic recombination endonuclease Rec12 Affinity Capture-WesternPMID:22841486
affinity capturesrec12meiotic recombination endonuclease Rec12 Affinity Capture-WesternPMID:22841486
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC32F12.02 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withcid1terminal uridylyltransferase Cid1 Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withmet8siroheme synthase Met8 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withngg1SAGA complex subunit Ngg1 Negative GeneticPMID:18818364
negative genetic interaction withalg5dolichyl-phosphate beta-glucosyltransferase Alg5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvms1Cdc48p-Npl4p-Vms1p AAA ATPase complex subunit involved in ER associated ubiquitin-dependent protein catabolic process Vms1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withyox1MBF complex corepressor Yox1 Negative GeneticPMID:22681890
negative genetic interaction withSPCC16C4.106-phosphogluconolactonase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtpp1trehalose-6-phosphate phosphatase Tpp1 Negative GeneticPMID:22681890
negative genetic interaction withsum2translation initiation inhibitor (predicted) Negative GeneticPMID:18818364
negative genetic interaction withsat1Golgi membrane exchange factor subunit Sat1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withhcr1translation initiation factor eIF3j (p35) Negative GeneticPMID:22681890
negative genetic interaction withmaf1repressor of RNA polymerase III Maf1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC15C4.06cubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withapt1adenine phosphoribosyltransferase (APRT) (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
negative genetic interaction withbtf3nascent polypeptide-associated complex beta subunit Negative GeneticPMID:22681890
negative genetic interaction withcgs2cAMP-specific phosphodiesterase Cgs2 Negative GeneticPMID:22681890
negative genetic interaction witharf6ADP-ribosylation factor, Arf family Arf6 Negative GeneticPMID:22681890
negative genetic interaction withubp3ubiquitin C-terminal hydrolase Ubp3 Negative GeneticPMID:18818364
negative genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:18818364
negative genetic interaction withzip2vacuolar membrane zinc transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withatp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpds5mitotic and meiotic cohesin-associated protein Pds5 Negative GeneticPMID:18818364
negative genetic interaction withpng2ING family homolog Png2 Negative GeneticPMID:18818364
negative genetic interaction withmms19Dos2 silencing complex subunit Mms19 Negative GeneticPMID:18818364
negative genetic interaction withlub1WD repeat protein Lub1 Negative GeneticPMID:18818364
negative genetic interaction witheca39branched chain amino acid aminotransferase Eca39 Negative GeneticPMID:22681890
negative genetic interaction withrpl240260S ribosomal protein L24 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Negative GeneticPMID:22681890
negative genetic interaction withpsl1cyclin pho85 family Psl1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withfrp1ferric-chelate reductase Frp1 Negative GeneticPMID:22681890
negative genetic interaction withclg1cyclin Clg1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcbp1CENP-B homolog Negative GeneticPMID:18818364
negative genetic interaction withalg9mannosyltransferase complex subunit Alg9 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withalg8glucosyltransferase Alg8 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhop1linear element associated protein Hop1 Negative GeneticPMID:18818364
negative genetic interaction withash2Ash2-trithorax family protein Negative GeneticPMID:18818364
negative genetic interaction withmug129Schizosaccharomyces specific protein Mug129 Negative GeneticPMID:22681890
negative genetic interaction withsdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:18818364
negative genetic interaction withrpl280160S ribosomal protein L27/L28 Negative GeneticPMID:22681890
negative genetic interaction withSPBC3B8.04csodium ion transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction witherg5C-22 sterol desaturase Erg5 Negative GeneticPMID:18818364
negative genetic interaction withubc6ubiquitin conjugating enzyme E2 Ubc6 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPBC16C6.03cribosome assembly protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpl170260S ribosomal protein L17 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpl430260S ribosomal protein L37a (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcia1histone chaperone Cia1 Negative GeneticPMID:18818364
negative genetic interaction withdom34peloto ortholog (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmed6mediator complex subunit Med6 Negative GeneticPMID:18818364
negative genetic interaction withcoq11ubiquinone biosynthesis protein Coq11 Negative GeneticPMID:22681890
negative genetic interaction withfus1formin Fus1 Negative GeneticPMID:22681890
negative genetic interaction withtrx2mitochondrial thioredoxin Trx2 Negative GeneticPMID:22681890
negative genetic interaction withmug81ATP-dependent RNA helicase Slh1, human ASCC3 ortholog (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPCC18.17cproteasome assembly chaperone (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhrd1synviolin family ubiquitin-protein ligase E3, Hrd1 Negative GeneticPMID:18818364
negative genetic interaction withrpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:18818364
negative genetic interaction withrps170240S ribosomal protein S17 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpuf1pumilio family RNA-binding protein Puf1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withryh1GTPase Ryh1 Negative GeneticPMID:18818364
positive genetic interaction withrds1conserved fungal protein Positive GeneticPMID:22681890
positive genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Positive GeneticPMID:18818364
positive genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Positive GeneticPMID:18818364
positive genetic interaction withftp105Ubp5 interacting protein Ftp105 Positive GeneticPMID:18818364
positive genetic interaction withapm3AP-3 adaptor complex subunit Apm3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPAC1399.04curacil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPAC9.02cpolyamine N-acetyltransferase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:18818364
positive genetic interaction withscs7sphingosine hydroxylase Scs7 Positive GeneticPMID:18818364
positive genetic interaction withmei4meiotic forkhead transcription factor Mei4 Positive GeneticPMID:18818364
positive genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Positive GeneticPMID:22681890
positive genetic interaction withwhi2phosphatase activator Whi2 Positive GeneticPMID:22681890
positive genetic interaction withelp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Positive GeneticPMID:22681890
phenotype enhanced bysgo1meiotic inner centromere protein, shugoshin, Sgo1 Phenotypic EnhancementPMID:21423721
enhances phenotype ofpst2Clr6 histone deacetylase complex subunit Pst2 Phenotypic EnhancementPMID:19547744
External References
Database Identifier Description
NBRP SPBC32F12.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC32F12.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC32F12.02 BioGRID Interaction Datasets
Expression Viewer SPBC32F12.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC32F12.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC32F12.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC32F12.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC32F12.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC32F12.02 Transcriptome Viewer (Bähler Lab)
GEO SPBC32F12.02 GEO profiles
PInt SPBC32F12.02 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC32F12.02 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC32F12.02 Fission yeast phenotypic data & analysis
Cyclebase SPBC32F12.02.1 Cell Cycle Data
SPD / RIKEN32/32B07Orfeome Localization Data
UniProtKB/SwissProtQ09150Meiotic recombination protein rec14
ModBaseQ09150Database of comparative protein structure models
STRINGQ09150Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596145Ski complex subunit Rec14
RefSeq mRNANM_001022064972h- Ski complex subunit Rec14 (rec14), mRNA

Literature for rec14

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016