rec14 (SPBC32F12.02)


Gene Standard Namerec14 Characterisation Statuspublished
Systematic IDSPBC32F12.02 Feature Typeprotein coding
Synonyms Name Description
ProductSki complex subunit Rec14 Product Size302aa, 32.94 kDa
Genomic Location Chromosome II, 2786858-2788406 (1549nt); CDS:2786978-2787939 (962nt)

Ensembl Gene Location
GO Biological Process
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
Ski complex3
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased sporulation frequencyrec14Δ101
slow vegetative cell population growthrec14-120340
viable vegetative cell populationrec14Δ3831

Cell Phenotype

Term NameGenotypesCount
abnormal linear element morphologyrec14Δ6
decreased meiotic recombinationrec14-12048
normal mitotic recombinationrec14-1207
viable vegetative cell with normal cell morphologyrec14Δ3099
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2786858..2787266, 2787320..2788406
Intron2787267..2787319
5' UTR2786858..2786977PMID:21511999
3' UTR2787940..2788406PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08662 Pfam IPR013979 Translation initiation factor, beta propellor-like domain 167 286 5
SM00320 SMART IPR001680 WD40 repeat 260 297 112
SM00320 SMART IPR001680 WD40 repeat 92 131 112
SM00320 SMART IPR001680 WD40 repeat 218 257 112
SM00320 SMART IPR001680 WD40 repeat 176 215 112
SM00320 SMART IPR001680 WD40 repeat 134 173 112
SM00320 SMART IPR001680 WD40 repeat 3 42 112
SM00320 SMART IPR001680 WD40 repeat 47 87 112
PS50294 Prosite Profiles IPR017986 WD40-repeat-containing domain 12 302 104
PS50082 Prosite Profiles IPR001680 WD40 repeat 225 266 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 183 224 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 99 131 100
PTHR22847 HMMPANTHER 29 294 13
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 13 298 145
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 29 298 135

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 109.06 Da
Charge -10.50
Codon Adaptation Index 0.42
Isoelectric point 4.80
Molecular weight 32.94 kDa
Number of residues 302
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0051321Northern assay evidencePMID:9258671

Quantitative Gene Expression

View graphical display of gene expression data for rec14 (SPBC32F12.02)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
16332during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14633during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14374during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14677during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14594during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4666.24during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2111.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.43during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
conserved in eukaryotes only2504
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPBC32F12.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC32F12.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC32F12.02 BioGRID Interaction Datasets
Expression Viewer SPBC32F12.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC32F12.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC32F12.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC32F12.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC32F12.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC32F12.02 Transcriptome Viewer (Bähler Lab)
GEO SPBC32F12.02 GEO profiles
PInt SPBC32F12.02 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC32F12.02 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC32F12.02 Fission yeast phenotypic data & analysis
Cyclebase SPBC32F12.02.1 Cell Cycle Data
SPD / RIKEN32/32B07Orfeome Localization Data
UniProtKB/SwissProtQ09150Meiotic recombination protein rec14
ModBaseQ09150Database of comparative protein structure models
STRINGQ09150Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596145Ski complex subunit Rec14
RefSeq mRNANM_001022064972h- Ski complex subunit Rec14 (rec14), mRNA
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveU39144ENA EMBL mapping
European Nucleotide ArchiveAAB71433ENA Protein Mapping
European Nucleotide ArchiveCAA19363ENA Protein Mapping
UniParcUPI00001334ADUniProt Archive

Literature for rec14

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016