rec14 (SPBC32F12.02)


Gene Standard Namerec14 Characterisation Statuspublished
Systematic IDSPBC32F12.02 Feature Typeprotein coding
Synonyms Name Description
ProductSki complex subunit Rec14 Product Size302aa, 32.94 kDa
Genomic Location Chromosome II, 2786858-2788406 (1549nt); CDS:2786978-2787939 (962nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006402mRNA catabolic processISOSGD:S000003181GO_REF:000002469
GO:0007131reciprocal meiotic recombinationIMPPMID:925867143
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0055087Ski complexISOSGD:S000003181GO_REF:00000243
GO:0005829cytosolIDAPMID:168233722316
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001234slow vegetative cell population growthMicroscopyrec14-120Not specifiedPMID:925867154
FYPO:0002060viable vegetative cell populationMicroscopyrec14ΔNullPMID:204732893760
Microscopyrec14ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000485decreased meiotic recombination10
expressivity FYPO_EXT:0000001Otherrec14-120Not specifiedPMID:9258671
FYPO:0000584decreased sporulationCell growth assayrec14ΔNullPMID:925867142
FYPO:0000503normal mitotic recombinationOtherrec14-120Not specifiedPMID:92586712
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopyrec14ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
127868582787266
227873202788406

UTRs

Region Coordinates Reference
five_prime_UTR2786858..2786977PMID:21511999
three_prime_UTR2787940..2788406PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00400 Pfam IPR001680 WD40 repeat 179 215 104
PF00400 Pfam IPR001680 WD40 repeat 102 131 104
PF00400 Pfam IPR001680 WD40 repeat 221 257 104
SM00320 SMART IPR001680 WD40 repeat 218 257 121
SM00320 SMART IPR001680 WD40 repeat 92 131 121
SM00320 SMART IPR001680 WD40 repeat 47 87 121
SM00320 SMART IPR001680 WD40 repeat 134 173 121
SM00320 SMART IPR001680 WD40 repeat 176 215 121
SM00320 SMART IPR001680 WD40 repeat 3 42 121
SM00320 SMART IPR001680 WD40 repeat 260 297 121
PS50294 Prosite Profiles IPR017986 WD40-repeat-containing domain 12 302 104
PS50082 Prosite Profiles IPR001680 WD40 repeat 183 224 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 99 131 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 225 266 100
PTHR22841 HMMPANTHER 1 299 3
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 13 298 143
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 29 298 135

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 109.06 Da
Charge -10.50
Isoelectric point 4.80
Molecular weight 32.94 kDa
Number of residues 302
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0051321Northern assay evidencePMID:9258671

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
16332during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
14633during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
14374during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
14677during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
2111.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
14594during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4666.24during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.43during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
1.5during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pnk1DNA kinase/phosphatase Pnk1 Positive GeneticPMID:18818364
cbp1CENP-B homolog Negative GeneticPMID:18818364
scs7sphingosine hydroxylase Scs7 Positive GeneticPMID:18818364
lub1WD repeat protein Lub1 Negative GeneticPMID:18818364
frp1ferric-chelate reductase Frp1 Negative GeneticPMID:22681890
btf3nascent polypeptide-associated complex beta subunit Negative GeneticPMID:22681890
vms1Cdc48p-Npl4p-Vms1p AAA ATPase complex subunit involved in ER associated ubiquitin-dependent protein catabolic process Vms1 (predicted) Negative GeneticPMID:22681890
eca39branched chain amino acid aminotransferase Eca39 Negative GeneticPMID:22681890
ubp3ubiquitin C-terminal hydrolase Ubp3 Negative GeneticPMID:18818364
cid1poly(A) polymerase Cid1 Negative GeneticPMID:22681890
png2ING family homolog Png2 Negative GeneticPMID:18818364
atp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
ftp105Ubp5 interacting protein Ftp105 Positive GeneticPMID:18818364
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:18818364
mug81ATP-dependent RNA helicase Slh1 (predicted) Negative GeneticPMID:18818364
SPCC18B5.06peloto ortholog (predicted) Negative GeneticPMID:22681890
arf6ADP-ribosylation factor, Arf family Arf6 Negative GeneticPMID:22681890
hop1linear element associated protein Hop1 Negative GeneticPMID:18818364
elp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:18818364
hrd1ubiquitin-protein ligase Hrd1, synviolin family Negative GeneticPMID:18818364
SPCC16C4.106-phosphogluconolactonase (predicted) Negative GeneticPMID:22681890
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
mms19Dos2 silencing complex subunit Mms19 Negative GeneticPMID:18818364
SPAC9.02cpolyamine N-acetyltransferase (predicted) Positive GeneticPMID:22681890
SPAC3A12.13ctranslation initiation factor eIF3j (p35) Negative GeneticPMID:22681890
SPAC1399.04curacil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
SPBC3B8.04cmembrane transporter (predicted) Negative GeneticPMID:22681890
rds1conserved fungal protein Positive GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:18818364
SPAC20H4.08phosphatase activator (predicted) Positive GeneticPMID:22681890
pds5cohesin-associated protein Pds5 Negative GeneticPMID:18818364
pst2Clr6 histone deacetylase complex subunit Pst2 Phenotypic EnhancementPMID:19547744
ash2Ash2-trithorax family protein Negative GeneticPMID:18818364
SPAC4D7.06csiroheme synthase (predicted) Negative GeneticPMID:22681890
alg9mannosyltransferase complex subunit Alg9 (predicted) Negative GeneticPMID:22681890
sat1Golgi membrane exchange factor subunit Sat1 (predicted) Negative GeneticPMID:18818364
pmc5mediator complex subunit Pmc5 Negative GeneticPMID:18818364
SPBC16C6.03cribosome assembly protein (predicted) Negative GeneticPMID:22681890
cgs2cAMP-specific phosphodiesterase Cgs2 Negative GeneticPMID:22681890
sgo1inner centromere protein, shugoshin, Sgo1 Phenotypic EnhancementPMID:21423721
erg5C-22 sterol desaturase Erg5 Negative GeneticPMID:18818364
mug129sequence orphan Negative GeneticPMID:22681890
rpl240260S ribosomal protein L24 (predicted) Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Positive GeneticPMID:22681890
mei4meiotic forkhead transcription factor Mei4 Positive GeneticPMID:18818364
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:18818364
rpl280160S ribosomal protein L27/L28 Negative GeneticPMID:22681890
SPCC18.17c26S proteasome non-ATPase regulatory subunit (predicted) Negative GeneticPMID:22681890
psl1cyclin pho85 family Psl1 (predicted) Negative GeneticPMID:22681890
rps170240S ribosomal protein S17 (predicted) Negative GeneticPMID:22681890
cia1histone chaperone Cia1 Negative GeneticPMID:18818364
SPBC15C4.06cubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:18818364
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:18818364
apt1adenine phosphoribosyltransferase (APRT) (predicted) Negative GeneticPMID:22681890
amk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:18818364
SPCC1840.09NAD dependent epimerase/dehydratase family protein Negative GeneticPMID:22681890
ulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Positive GeneticPMID:22681890
ubc6ubiquitin conjugating enzyme Ubc6 (predicted) Negative GeneticPMID:18818364
alg5dolichyl-phosphate beta-glucosyltransferase Alg5 (predicted) Negative GeneticPMID:22681890
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Positive GeneticPMID:18818364
ssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:18818364
mug80cyclin Clg1 (predicted) Negative GeneticPMID:22681890
rpl430260S ribosomal protein L37a (predicted) Negative GeneticPMID:22681890
rpl170260S ribosomal protein L17 (predicted) Negative GeneticPMID:22681890
yox1MBF complex corepressor Yox1 Negative GeneticPMID:22681890
tpp1trehalose-6-phosphate phosphatase Tpp1 Negative GeneticPMID:22681890
trx2mitochondrial thioredoxin Trx2 Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
zip2vacuolar membrane zinc transporter (predicted) Negative GeneticPMID:22681890
maf1repressor of RNA polymerase III Maf1 Negative GeneticPMID:22681890
ryh1GTPase Ryh1 Negative GeneticPMID:18818364
fus1formin Fus1 Negative GeneticPMID:22681890
rnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Negative GeneticPMID:22681890
apm3AP-3 adaptor complex subunit Apm3 (predicted) Positive GeneticPMID:22681890
alg8glucosyltransferase Alg8 (predicted) Negative GeneticPMID:22681890
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:18818364
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
mde2Mde2 protein Two-hybridPMID:22841486
Affinity Capture-Western
Reconstituted Complex
Two-hybridPMID:20364342
rec12endonuclease Rec12 Two-hybridPMID:22841486
Affinity Capture-Western
Two-hybridPMID:20364342
External References
Database Identifier Description
NBRP SPBC32F12.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC32F12.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC32F12.02 BioGRID Interaction Datasets
Expression Viewer SPBC32F12.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC32F12.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC32F12.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC32F12.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC32F12.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC32F12.02 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC32F12.02 Cell Cycle Data
GEO SPBC32F12.02 GEO profiles
PInt SPBC32F12.02 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2540221Ski complex subunit Rec14
WikiGene2540221Ski complex subunit Rec14
SPD / RIKEN32/32B07Orfeome Localization Data
UniProtKB/SwissProtQ09150Meiotic recombination protein rec14
ModBaseQ09150Database of comparative protein structure models
STRINGQ09150Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596145Ski complex subunit Rec14
RefSeq mRNANM_001022064972h- Ski complex subunit Rec14 (rec14), mRNA
European Nucleotide ArchiveAAB71433ENA Protein Mapping
European Nucleotide ArchiveAAB71433.1ENA Protein Mapping
European Nucleotide ArchiveCAA19363ENA Protein Mapping
European Nucleotide ArchiveCAA19363.1ENA Protein Mapping
UniParcUPI00001334ADUniProt Archive

Literature for rec14

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014