sfc3 (SPBC336.07)


Gene Standard Namesfc3 Characterisation Statuspublished
Systematic IDSPBC336.07 Feature Typeprotein coding
Synonyms Name Description
Producttranscription factor TFIIIC complex B box binding subunit Sfc3 Product Size1339aa, 153.64 kDa
Genomic Location Chromosome II, 2750876-2755161 (4286nt); CDS:2750915-2754934 (4020nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
chromatin insulator sequence binding4
Annotation ExtensionEvidenceWith/FromReference
core RNA polymerase III binding transcription factor activity7
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
transcription initiation from RNA polymerase III promoter11
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear envelope136
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
transcription factor TFIIIC complex6
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationsfc3ΔNull1428

Cell Phenotype

Term NameAlleleExpressionCount
decreased protein localization to chromatin at tRNA genes3
affecting sfc6sfc3-1Not specified
affecting rpc25sfc3-1Not specified
inviable vegetative cell, abnormal cell shape, normal cell sizesfc3ΔNull199
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
127508762755161

UTRs

Region Coordinates Reference
five_prime_UTR2750876..2750914PMID:21511999
three_prime_UTR2754935..2755161PMID:21511999
mRNA2750876..2755161
exon2750915..2754934
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04182 Pfam IPR007309 B-block binding subunit of TFIIIC 120 187 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0002147AT hook proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002147

Protein Properties

Ave. residue weight 114.74 Da
Charge 19.50
Isoelectric point 8.22
Molecular weight 153.64 kDa
Number of residues 1339
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS498
present during mitotic M phaseS501
S595
present during mitotic M phaseS494
present during mitotic M phaseS502
present during mitotic M phaseS596
level fluctuates during mitotic cell cycleS498
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S494 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S498 PMID:24763107
present during mitotic M phase experimental evidence S498 PMID:21712547
present during mitotic M phase experimental evidence S501 PMID:21712547
present during mitotic M phase experimental evidence S502 PMID:21712547
experimental evidence S595 PMID:24763107
present during mitotic M phase experimental evidence S596 PMID:21712547
O-phosphorylated residueS502 1751
Annotation ExtensionEvidenceResidueReference
IDA S502 PMID:23297348
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1937during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2035during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1393during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2091during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1718during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity capturessfc1transcription factor TFIIIC complex A box associated subunit Sfc1 Affinity Capture-WesternPMID:10906331
affinity capturessfc4transcription factor TFIIIC complex subunit Sfc4 Affinity Capture-WesternPMID:10906331
affinity capturessfc6transcription factor TFIIIC complex subunit Sfc6 Affinity Capture-WesternPMID:10906331
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
enhances phenotype ofcut3condensin complex subunit Cut3 Phenotypic EnhancementPMID:19910488
synthetically rescuescut3condensin complex subunit Cut3 Synthetic RescuePMID:21633354
synthetic growth defect withswd22WD repeat protein (predicted) Synthetic Growth DefectPMID:24945319
External References
Database Identifier Description
NBRP SPBC336.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.07 BioGRID Interaction Datasets
Expression Viewer SPBC336.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC336.07 Cell Cycle Data
GEO SPBC336.07 GEO profiles
PInt SPBC336.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC336.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC336.07 Fission yeast phenotypic data & analysis
SPD / RIKEN48/48F10Orfeome Localization Data
UniProtKB/SwissProtQ9UST7Transcription factor tau subunit sfc3
ModBaseQ9UST7Database of comparative protein structure models
STRINGQ9UST7Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596127transcription factor TFIIIC complex B box binding subunit Sfc3
RefSeq mRNANM_001022045972h- transcription factor TFIIIC complex B box binding subunit Sfc3 (sfc3), mRNA
European Nucleotide ArchiveCAB58159.1ENA Protein Mapping
UniParcUPI0000069A03UniProt Archive

Literature for sfc3

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015