sfc3 (SPBC336.07)


Gene Standard Namesfc3 Characterisation Statuspublished
Systematic IDSPBC336.07 Feature Typeprotein coding
Synonyms Name Description
Producttranscription factor TFIIIC complex B box binding subunit Sfc3 Product Size1339aa, 153.64 kDa
Genomic Location Chromosome II, 2750876-2755161 (4286nt); CDS:2750915-2754934 (4020nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
chromatin insulator sequence binding4
Annotation ExtensionEvidenceWith/FromReference
transcription factor activity, core RNA polymerase III binding8
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic chromosome arm chromatin condensation1
Annotation ExtensionEvidenceWith/FromReference
transcription initiation from RNA polymerase III promoter11
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear envelope129
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
transcription factor TFIIIC complex6
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationsfc3Δ1451

Cell Phenotype

Term NameGenotypesCount
decreased protein localization to chromatin at tRNA genes3
affects localization of sfc6sfc3-1
affects localization of rpc25sfc3-1
inviable vegetative cell, abnormal cell shape, normal cell sizesfc3Δ199

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
viable vegetative cell populationsfc3-1, cut3-477

Cell Phenotype

Term NameGenotypes
decreased mitotic chromosome condensationsfc3-1, cut3-477
decreased vegetative cell growthcsm1Δ, sfc3-1
normal vegetative cell growthcnp3C (fusion-cnd2), sfc3Δ
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2750876..2755161
mRNA2750876..2755161
5' UTR2750876..2750914PMID:21511999
CDS2750915..2754934
3' UTR2754935..2755161PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04182 Pfam IPR007309 B-block binding subunit of TFIIIC 120 187 1
PTHR15180 HMMPANTHER 1 323 1
PTHR15180 HMMPANTHER 344 612 1

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0002147AT hook proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002147

Protein Properties

Ave. residue weight 114.74 Da
Charge 19.50
Codon Adaptation Index 0.40
Isoelectric point 8.22
Molecular weight 153.64 kDa
Number of residues 1339
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS501
present during mitotic M phaseS596
present during mitotic M phaseS498
present during mitotic M phaseS494
present during mitotic M phaseS502
S595
level fluctuates during mitotic cell cycleS498
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S501 PMID:21712547
present during mitotic M phase experimental evidence S596 PMID:21712547
present during mitotic M phase experimental evidence S498 PMID:21712547
present during mitotic M phase experimental evidence S494 PMID:21712547
present during mitotic M phase experimental evidence S502 PMID:21712547
experimental evidence S595 PMID:24763107
level fluctuates during mitotic cell cycle experimental evidence S498 PMID:24763107
O-phosphorylated residueS502 1757
Annotation ExtensionEvidenceResidueReference
IDA S502 PMID:23297348
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for sfc3 (SPBC336.07)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1937during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2035during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1393during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2091during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1718during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC336.07 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturessfc6transcription factor TFIIIC complex subunit Sfc6 Affinity Capture-WesternPMID:10906331
affinity capturessfc4transcription factor TFIIIC complex subunit Sfc4 Affinity Capture-WesternPMID:10906331
affinity capturessfc1transcription factor TFIIIC complex A box associated subunit Sfc1 Affinity Capture-WesternPMID:10906331
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC336.07 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withswd22mRNA cleavage and polyadenylation specificity factor complex subunit, WD repeat protein Swd22 Synthetic Growth DefectPMID:24945319
enhances phenotype ofcut3condensin complex SMC subunit Smc4 Phenotypic EnhancementPMID:19910488
synthetically rescuescut3condensin complex SMC subunit Smc4 Synthetic RescuePMID:21633354
External References
Database Identifier Description
NBRP SPBC336.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.07 BioGRID Interaction Datasets
Expression Viewer SPBC336.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.07 Transcriptome Viewer (Bähler Lab)
GEO SPBC336.07 GEO profiles
PInt SPBC336.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC336.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC336.07 Fission yeast phenotypic data & analysis
Cyclebase SPBC336.07.1 Cell Cycle Data
SPD / RIKEN48/48F10Orfeome Localization Data
UniProtKB/SwissProtQ9UST7Transcription factor tau subunit sfc3
ModBaseQ9UST7Database of comparative protein structure models
STRINGQ9UST7Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596127transcription factor TFIIIC complex B box binding subunit Sfc3
RefSeq mRNANM_001022045972h- transcription factor TFIIIC complex B box binding subunit Sfc3 (sfc3), mRNA

Literature for sfc3

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016