sfc3 (SPBC336.07)


Gene Standard Namesfc3 Characterisation Statuspublished
Systematic IDSPBC336.07 Feature Typeprotein coding
Synonyms Name Description
Producttranscription factor TFIIIC complex B box binding subunit Sfc3 Product Size1339aa, 153.64 kDa
Genomic Location Chromosome II, 2750876-2755161 (4286nt); CDS:2750915-2754934 (4020nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0043035chromatin insulator sequence binding4
contributes_toIDAPMID:16751097
GO:0000995core RNA polymerase III binding transcription factor activity7
contributes_toIDAPMID:10906331
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006384transcription initiation from RNA polymerase III promoterIDAPMID:1090633111
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005635nuclear envelopeIDAPMID:16823372137
GO:0005634nucleusIDAPMID:168233722740
GO:0000127transcription factor TFIIIC complexIDAPMID:109063316
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopysfc3ΔNullPMID:204732891338
Microscopysfc3ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001511inviable vegetative cell, abnormal cell shape, normal cell size199
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopysfc3ΔNullPECO:0000137, PECO:0000005PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
127508762755161

UTRs

Region Coordinates Reference
five_prime_UTR2750876..2750914PMID:21511999
three_prime_UTR2754935..2755161PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04182 Pfam IPR007309 B-block binding subunit of TFIIIC 120 187 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0002147AT hook proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002147

Protein Properties

Ave. residue weight 114.74 Da
Charge 19.50
Isoelectric point 8.22
Molecular weight 153.64 kDa
Number of residues 1339
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS502PMID:217125471670
present during mitotic M phase
experimental evidenceS595PMID:24763107
level fluctuates during mitotic cell cycleexperimental evidenceS498PMID:24763107
present during mitotic M phaseexperimental evidenceS501PMID:21712547
present during mitotic M phaseexperimental evidenceS498PMID:21712547
present during mitotic M phaseexperimental evidenceS596PMID:21712547
present during mitotic M phaseexperimental evidenceS494PMID:21712547
MOD:01455O-phosphorylated residueIDAS502PMID:232973481749
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1937during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2035during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1393during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2091during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1718during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
SPAC824.04WD repeat protein (predicted) Synthetic Growth DefectPMID:24945319
cut3condensin complex subunit Cut3 Phenotypic EnhancementPMID:19910488
Synthetic RescuePMID:21633354
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
sfc6transcription factor TFIIIC complex subunit Sfc6 Affinity Capture-WesternPMID:10906331
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
sfc4transcription factor TFIIIC complex subunit Sfc4 Affinity Capture-WesternPMID:10906331
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
sfc1transcription factor TFIIIC complex A box associated subunit Sfc1 Affinity Capture-WesternPMID:10906331
External References
Database Identifier Description
NBRP SPBC336.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.07 BioGRID Interaction Datasets
Expression Viewer SPBC336.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC336.07 Cell Cycle Data
GEO SPBC336.07 GEO profiles
PInt SPBC336.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC336.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC336.07 Fission yeast phenotypic data & analysis
SPD / RIKEN48/48F10Orfeome Localization Data
UniProtKB/SwissProtQ9UST7Transcription factor tau subunit sfc3
ModBaseQ9UST7Database of comparative protein structure models
STRINGQ9UST7Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596127transcription factor TFIIIC complex B box binding subunit Sfc3
RefSeq mRNANM_001022045972h- transcription factor TFIIIC complex B box binding subunit Sfc3 (sfc3), mRNA
European Nucleotide ArchiveCAB58159.1ENA Protein Mapping
UniParcUPI0000069A03UniProt Archive

Literature for sfc3

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014