tif512 (SPBC336.10c)


Gene Standard Nametif512 Characterisation Statusbiological role inferred
Systematic IDSPBC336.10c Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eIF5A (predicted) Product Size169aa, 18.56 kDa
Genomic Location Chromosome II, 2759294-2758645 (650nt); CDS:2759270-2758761 (510nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ribosome binding10
Annotation ExtensionEvidenceWith/FromReference
RNA binding580
Annotation ExtensionEvidenceWith/FromReference
translation elongation factor activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translational elongation194
Annotation ExtensionEvidenceWith/FromReference
translational frameshifting2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
cytosolic ribosome199
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationtif512Δ3831

Cell Phenotype

Term NameGenotypesCount
increased protein acetylation20
affecting tif512tif512+
viable vegetative cell with normal cell morphologytif512Δ3099
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in hst2 Sirtuin family histone deacetylase Hst2
FYPO affected by mutation in tif512 translation elongation factor eIF5A (predicted)
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2759294..2758645
5' UTR2759294..2759271PMID:21511999
3' UTR2758760..2758645AU011603
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01287 Pfam IPR020189 Translation elongation factor, IF5A C-terminal 97 164 2
PS00302 Prosite Patterns IPR019769 Translation elongation factor, IF5A, hypusine site 62 69 2
PTHR11673 HMMPANTHER IPR001884 Translation elongation factor IF5A 14 169 2
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 102 167 48
2.30.30.30 Gene3D IPR014722 Ribosomal protein L2 domain 2 26 101 13
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 98 167 57
SSF50104 SuperFamily IPR008991 Translation protein SH3-like domain 27 105 16
PIRSF003025 PIRSF IPR001884 Translation elongation factor IF5A 13 168 2
TIGR00037 tigrfam IPR001884 Translation elongation factor IF5A 30 160 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.84 Da
Charge -8.50
Codon Adaptation Index 0.71
Isoelectric point 4.72
Molecular weight 18.56 kDa
Number of residues 169
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1675
present during cellular response to thiabendazoleS87
present during cellular response to thiabendazoleS89
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S87 PMID:18257517
present during cellular response to thiabendazole IDA S89 PMID:18257517
hypusine 1
Annotation ExtensionEvidenceResidueReference
ISS PB_REF:0000001
acetylated residue 14
Annotation ExtensionEvidenceResidueReference
IDA PMID:18292091
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for tif512 (SPBC336.10c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
18754during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
17570during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
17536during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
20159during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
19343during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14616.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
110during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
9.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999433
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
conserved in archaea238
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPBC336.10c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withtas3RITS complex subunit 3 Positive GeneticPMID:22681890
positive genetic interaction withago1argonaute Positive GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC336.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.10c BioGRID Interaction Datasets
Expression Viewer SPBC336.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.10c Transcriptome Viewer (Bähler Lab)
GEO SPBC336.10c GEO profiles
PInt SPBC336.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC336.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC336.10c Fission yeast phenotypic data & analysis
Cyclebase SPBC336.10c.1 Cell Cycle Data
SPD / RIKEN31/31D10Orfeome Localization Data
UniProtKB/SwissProtQ9UST4Eukaryotic translation initiation factor 5A-2
ModBaseQ9UST4Database of comparative protein structure models
STRINGQ9UST4Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596130translation elongation factor eIF5A (predicted)
RefSeq mRNANM_001022048972h- translation elongation factor eIF5A (predicted) (tif512), mRNA
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveCAB58162ENA Protein Mapping
UniParcUPI000228F3A8UniProt Archive

Literature for tif512

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016