tif512 (SPBC336.10c)


Gene Standard Nametif512 Characterisation Statusbiological_role_inferred
Systematic IDSPBC336.10c Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eIF5A (predicted) Product Size169aa, 18.56 kDa
Genomic Location Chromosome II, 2759294-2758645 (650nt); CDS:2759270-2758761 (510nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003723RNA bindingISOSGD:S000000760GO_REF:0000024628
GO:0043022ribosome bindingISOSGD:S000000760GO_REF:00000249
GO:0003746translation elongation factor activityISOSGD:S000000760GO_REF:000002415
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002182cytoplasmic translational elongationNASGO_REF:0000051193
GO:0002184cytoplasmic translational terminationNASGO_REF:00000518
GO:0008612peptidyl-lysine modification to peptidyl-hypusineIEAIPR001884GO_REF:00000024
GO:0045901positive regulation of translational elongationISOSGD:S000000760GO_REF:00000242
GO:0045905positive regulation of translational terminationISOSGD:S000000760GO_REF:00000242
GO:0006452translational frameshiftingISOSGD:S000000760GO_REF:00000242
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0022626cytosolic ribosomeISOSGD:S000000760GO_REF:0000024199
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopytif512ΔNullPECO:0000005, PECO:0000137PMID:236978063730
Microscopytif512ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopytif512ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
127592942758645

UTRs

Region Coordinates Reference
five_prime_UTR2759294..2759271PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00467 Pfam IPR005824 KOW 41 72 9
PF01287 Pfam IPR020189 Translation elongation factor, IF5A C-terminal 97 164 2
PS00302 Prosite Patterns IPR019769 Translation elongation factor, IF5A, hypusine site 62 69 2
PTHR11673 HMMPANTHER IPR001884 Translation elongation factor IF5A 15 169 2
2.30.30.30 Gene3D IPR014722 Ribosomal protein L2 domain 2 26 101 13
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 102 167 48
SSF50104 SuperFamily IPR008991 Translation protein SH3-like domain 27 105 16
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 98 167 57
PIRSF003025 PIRSF IPR001884 Translation elongation factor IF5A 13 168 2
TIGR00037 tigrfam IPR001884 Translation elongation factor IF5A 30 160 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.84 Da
Charge -8.50
Isoelectric point 4.72
Molecular weight 18.56 kDa
Number of residues 169
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS89PMID:182575171669
present during cellular response to thiabendazole
present during cellular response to thiabendazoleIDAS87PMID:18257517
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
18754during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
17570during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
17536during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
20159during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
NDduring GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
19343during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
14616.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
110during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
9.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999433
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
SPCC1020.07haloacid dehalogenase-like hydrolase Positive GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
php3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Positive GeneticPMID:22681890
sts5RNB-like protein Positive GeneticPMID:22681890
atg6beclin family protein, involved in autophagy Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
pst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
rad9checkpoint clamp complex protein Rad9 Negative GeneticPMID:22681890
ash2Ash2-trithorax family protein Negative GeneticPMID:22681890
epe1Jmjc domain chromatin associated protein Epe1 Negative GeneticPMID:22681890
ppa2serine/threonine protein phosphatase Ppa2 Negative GeneticPMID:22681890
sif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
cwf23DNAJ domain protein Cwf23 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
far8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPBC336.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.10c BioGRID Interaction Datasets
Expression Viewer SPBC336.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC336.10c Cell Cycle Data
GEO SPBC336.10c GEO profiles
PInt SPBC336.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC336.10c Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2540232translation elongation factor eIF5A
EntrezGene2540232translation elongation factor eIF5A
SPD / RIKEN31/31D10Orfeome Localization Data
UniProtKB/SwissProtQ9UST4Eukaryotic translation initiation factor 5A-2
ModBaseQ9UST4Database of comparative protein structure models
STRINGQ9UST4Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596130translation elongation factor eIF5A
RefSeq mRNANM_001022048972h- translation elongation factor eIF5A (tif512), mRNA
European Nucleotide ArchiveCAB58162ENA Protein Mapping
European Nucleotide ArchiveCAB58162.2ENA Protein Mapping
UniParcUPI000228F3A8UniProt Archive

Literature for tif512

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014