tif512 (SPBC336.10c)


Gene Standard Nametif512 Characterisation Statusbiological_role_inferred
Systematic IDSPBC336.10c Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eIF5A (predicted) Product Size169aa, 18.56 kDa
Genomic Location Chromosome II, 2759294-2758645 (650nt); CDS:2759270-2758761 (510nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ribosome binding9
Annotation ExtensionEvidenceWith/FromReference
RNA binding646
Annotation ExtensionEvidenceWith/FromReference
translation elongation factor activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translational elongation194
Annotation ExtensionEvidenceWith/FromReference
translational frameshifting2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
cytosolic ribosome200
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopytif512ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopytif512ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopytif512ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
127592942758645

UTRs

Region Coordinates Reference
five_prime_UTR2759294..2759271PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01287 Pfam IPR020189 Translation elongation factor, IF5A C-terminal 97 164 2
PF00467 Pfam IPR005824 KOW 41 72 9
PS00302 Prosite Patterns IPR019769 Translation elongation factor, IF5A, hypusine site 62 69 2
PTHR11673 HMMPANTHER IPR001884 Translation elongation factor IF5A 14 168 2
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 102 167 48
2.30.30.30 Gene3D IPR014722 Ribosomal protein L2 domain 2 26 101 13
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 98 167 57
SSF50104 SuperFamily IPR008991 Translation protein SH3-like domain 27 105 16
PIRSF003025 PIRSF IPR001884 Translation elongation factor IF5A 13 168 2
TIGR00037 tigrfam IPR001884 Translation elongation factor IF5A 30 160 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.84 Da
Charge -8.50
Isoelectric point 4.72
Molecular weight 18.56 kDa
Number of residues 169
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS89PMID:182575171670
present during cellular response to thiabendazole
present during cellular response to thiabendazoleIDAS87PMID:18257517
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
18754during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
17570during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
17536during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
20159during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
19343during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
14616.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
110during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
9.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
far8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
ams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
SPCC1020.07haloacid dehalogenase-like hydrolase Positive GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
php3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Positive GeneticPMID:22681890
sts5RNB-like protein Positive GeneticPMID:22681890
atg6beclin family protein, involved in autophagy Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
pst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
rad9checkpoint clamp complex protein Rad9 Negative GeneticPMID:22681890
ash2Ash2-trithorax family protein Negative GeneticPMID:22681890
epe1Jmjc domain chromatin associated protein Epe1 Negative GeneticPMID:22681890
ppa2serine/threonine protein phosphatase Ppa2 Negative GeneticPMID:22681890
sif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
cwf23DNAJ domain protein Cwf23 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC336.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.10c BioGRID Interaction Datasets
Expression Viewer SPBC336.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC336.10c Cell Cycle Data
GEO SPBC336.10c GEO profiles
PInt SPBC336.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC336.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC336.10c Fission yeast phenotypic data & analysis
SPD / RIKEN31/31D10Orfeome Localization Data
UniProtKB/SwissProtQ9UST4Eukaryotic translation initiation factor 5A-2
ModBaseQ9UST4Database of comparative protein structure models
STRINGQ9UST4Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596130translation elongation factor eIF5A (predicted)
RefSeq mRNANM_001022048972h- translation elongation factor eIF5A (predicted) (tif512), mRNA
European Nucleotide ArchiveCAB58162.2ENA Protein Mapping
UniParcUPI000228F3A8UniProt Archive

Literature for tif512

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014