tif512 (SPBC336.10c)


Gene Standard Nametif512 Characterisation Statusbiological_role_inferred
Systematic IDSPBC336.10c Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eIF5A (predicted) Product Size169aa, 18.56 kDa
Genomic Location Chromosome II, 2759294-2758645 (650nt); CDS:2759270-2758761 (510nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ribosome binding9
Annotation ExtensionEvidenceWith/FromReference
RNA binding569
Annotation ExtensionEvidenceWith/FromReference
translation elongation factor activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translational elongation193
Annotation ExtensionEvidenceWith/FromReference
translational frameshifting2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
cytosolic ribosome199
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationtif512ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologytif512ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2759294..2758645
mRNA2759294..2758645
5' UTR2759294..2759271PMID:21511999
CDS2759270..2758761
3' UTR2758760..2758645AU011603
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01287 Pfam IPR020189 Translation elongation factor, IF5A C-terminal 97 164 2
PF00467 Pfam IPR005824 KOW 41 72 9
PS00302 Prosite Patterns IPR019769 Translation elongation factor, IF5A, hypusine site 62 69 2
PTHR11673 HMMPANTHER IPR001884 Translation elongation factor IF5A 15 169 2
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 102 167 48
2.30.30.30 Gene3D IPR014722 Ribosomal protein L2 domain 2 26 101 13
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 98 167 57
SSF50104 SuperFamily IPR008991 Translation protein SH3-like domain 27 105 16
PIRSF003025 PIRSF IPR001884 Translation elongation factor IF5A 13 168 2
TIGR00037 tigrfam IPR001884 Translation elongation factor IF5A 30 160 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.84 Da
Charge -8.50
Isoelectric point 4.72
Molecular weight 18.56 kDa
Number of residues 169
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during cellular response to thiabendazoleS89
present during cellular response to thiabendazoleS87
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S87 PMID:18257517
present during cellular response to thiabendazole IDA S89 PMID:18257517
hypusine 1
Annotation ExtensionEvidenceResidueReference
ISS PMID:
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
18754during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
17570during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
17536during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
20159during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
19343during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
14616.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
110during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
9.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured byfar8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
negative genetic interaction withash2Ash2-trithorax family protein Negative GeneticPMID:22681890
positive genetic interaction withask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
positive genetic interaction withatg6beclin family protein, involved in autophagy Positive GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcwf23DNAJ domain protein Cwf23 Positive GeneticPMID:22681890
negative genetic interaction withepe1Jmjc domain chromatin associated protein Epe1 Negative GeneticPMID:22681890
positive genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
negative genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
negative genetic interaction withphp3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction withppa2serine/threonine protein phosphatase Ppa2 Negative GeneticPMID:22681890
positive genetic interaction withprz1calcineurin responsive transcription factor Prz1 Positive GeneticPMID:22681890
negative genetic interaction withpst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
negative genetic interaction withrad9checkpoint clamp complex protein Rad9 Negative GeneticPMID:22681890
negative genetic interaction withsif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
positive genetic interaction withSPCC1020.07haloacid dehalogenase-like hydrolase Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC336.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.10c BioGRID Interaction Datasets
Expression Viewer SPBC336.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC336.10c Cell Cycle Data
GEO SPBC336.10c GEO profiles
PInt SPBC336.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC336.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC336.10c Fission yeast phenotypic data & analysis
SPD / RIKEN31/31D10Orfeome Localization Data
UniProtKB/SwissProtQ9UST4Eukaryotic translation initiation factor 5A-2
ModBaseQ9UST4Database of comparative protein structure models
STRINGQ9UST4Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596130translation elongation factor eIF5A (predicted)
RefSeq mRNANM_001022048972h- translation elongation factor eIF5A (predicted) (tif512), mRNA
European Nucleotide ArchiveCAB58162.2ENA Protein Mapping
UniParcUPI000228F3A8UniProt Archive

Literature for tif512

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015