pic1 (SPBC336.15)


Gene Standard Namepic1 Characterisation Statuspublished
Systematic IDSPBC336.15 Feature Typeprotein coding
SynonymsSPBC685.01 Name Description
ProductINCENP, Pic1 Product Size1018aa, 114.48 kDa
Genomic Location Chromosome II, 2769046-2772538 (3493nt); CDS:2769271-2772370 (3100nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding848
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic cytokinesis134
Annotation ExtensionEvidenceWith/FromReference
mitotic sister chromatid segregation194
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
chromosome passenger complex4
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome inner kinetochore11
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle258
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationpic1ΔNull1427
resistance to hydrogen peroxide72
expressivity FYPO_EXT:0000001pic1ΔNull
sensitive to cadmium246
expressivity FYPO_EXT:0000002pic1ΔNull
sensitive to caffeine108
expressivity FYPO_EXT:0000001pic1ΔNull
sensitive to hydrogen peroxide137
expressivity FYPO_EXT:0000003pic1ΔNull

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclepic1ΔNull794
inviable after spore germination with elongated germ tube163
penetrance FYPO_EXT:0000001pic1ΔNull
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
127690462769428
227694722772538

UTRs

Region Coordinates Reference
five_prime_UTR2769046..2769270PMID:21511999
three_prime_UTR2772371..2772538PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03941 Pfam IPR005635 Inner centromere protein, ARK-binding domain 921 980 1
PTHR13142:SF1 HMMPANTHER 1 1018 1
PTHR13142 HMMPANTHER 1 1018 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.45 Da
Charge 44.50
Isoelectric point 10.22
Molecular weight 114.48 kDa
Number of residues 1018
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS644
present during mitotic M phaseS134
level fluctuates during mitotic cell cycleS361
present during mitotic M phaseS885
present during mitotic M phaseS361
present during mitotic M phaseS943
present during mitotic M phaseS295
present during mitotic M phaseS296
present during mitotic M phaseS327
present during mitotic M phaseS305
present during mitotic M phaseS947
S326, S327, S525, S943, S947
present during mitotic M phaseS525
present during mitotic M phaseS326
present during mitotic M phaseS475
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S134 PMID:21712547
present during mitotic M phase experimental evidence S295 PMID:21712547
present during mitotic M phase experimental evidence S296 PMID:21712547
present during mitotic M phase experimental evidence S305 PMID:21712547
experimental evidence S326 PMID:24763107
present during mitotic M phase experimental evidence S326 PMID:21712547
experimental evidence S327 PMID:24763107
present during mitotic M phase experimental evidence S327 PMID:21712547
present during mitotic M phase experimental evidence S361 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S361 PMID:24763107
present during mitotic M phase experimental evidence S475 PMID:21712547
experimental evidence S525 PMID:24763107
present during mitotic M phase experimental evidence S525 PMID:21712547
present during mitotic M phase experimental evidence S644 PMID:21712547
present during mitotic M phase experimental evidence S885 PMID:21712547
experimental evidence S943 PMID:24763107
present during mitotic M phase experimental evidence S943 PMID:21712547
experimental evidence S947 PMID:24763107
present during mitotic M phase experimental evidence S947 PMID:21712547
O-phospho-L-threonine 693
present during mitotic M phaseT366
present during mitotic M phaseT357
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T357 PMID:21712547
present during mitotic M phase experimental evidence T366 PMID:21712547
O-phosphorylated residueS75, S156, S171, S361, S497, S947, T61, T71 1751
Annotation ExtensionEvidenceResidueReference
IDA S75 PMID:23297348
IDA S156 PMID:23297348
IDA S171 PMID:23297348
IDA S361 PMID:23297348
IDA S497 PMID:23297348
IDA S947 PMID:23297348
IDA T61 PMID:23297348
IDA T71 PMID:23297348
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
337during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
521.54during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.89during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure may be incorrect15
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
ark1aurora-B kinase Ark1 Affinity Capture-WesternPMID:17301288
Two-hybridPMID:11950927
bir1survivin, Bir1 Affinity Capture-WesternPMID:17301288
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
swi6HP1 family chromodomain protein Swi6 Synthetic Growth DefectPMID:17322402
Synthetic LethalityPMID:22264609
mde4monopolin-like complex subunit Mde4 Synthetic LethalityPMID:22264609
bir1survivin, Bir1 Dosage RescuePMID:16199877
ark1aurora-B kinase Ark1 Dosage RescuePMID:11950927
External References
Database Identifier Description
NBRP SPBC336.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.15 BioGRID Interaction Datasets
Expression Viewer SPBC336.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.15 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC336.15 Cell Cycle Data
GEO SPBC336.15 GEO profiles
PInt SPBC336.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC336.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC336.15 Fission yeast phenotypic data & analysis
SPD / RIKEN47/47G12Orfeome Localization Data
UniProtKB/SwissProtQ1MTN3Inner centromere protein-related protein pic1
ModBaseQ1MTN3Database of comparative protein structure models
STRINGQ1MTN3Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596135INCENP, Pic1
RefSeq mRNANM_001022053972h- INCENP, Pic1 (pic1), mRNA
European Nucleotide ArchiveCAB58167.1ENA Protein Mapping
UniParcUPI000006C889UniProt Archive

Literature for pic1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015