pic1 (SPBC336.15)


Gene Standard Namepic1 Characterisation Statuspublished
Systematic IDSPBC336.15 Feature Typeprotein coding
SynonymsSPBC685.01 Name Description
Productkinetochore protein, INCENP ortholog Pic1 Product Size1018aa, 114.48 kDa
Genomic Location Chromosome II, 2769046-2772538 (3493nt); CDS:2769271-2772370 (3100nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding863
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic cytokinesis106
Annotation ExtensionEvidenceWith/FromReference
mitotic sister chromatid segregation184
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
chromosome passenger complex4
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome inner kinetochore10
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle269
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationpic1Δ1453
resistance to hydrogen peroxidepic1Δ75
sensitive to cadmiumpic1Δ249
sensitive to caffeine during vegetative growthpic1Δ110
sensitive to hydrogen peroxidepic1Δ154

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclepic1Δ858
inviable after spore germination with elongated germ tubepic1Δ164
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2769046..2769428, 2769472..2772538
Intron2769429..2769471
5' UTR2769046..2769270PMID:21511999
3' UTR2772371..2772538PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03941 Pfam IPR005635 Inner centromere protein, ARK-binding domain 921 979 1
PTHR13142 HMMPANTHER 875 1018 1
PTHR13142:SF1 HMMPANTHER 875 1018 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.45 Da
Charge 44.50
Codon Adaptation Index 0.39
Isoelectric point 10.22
Molecular weight 114.48 kDa
Number of residues 1018
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS475
present during mitotic M phaseS943
present during mitotic M phaseS295
present during mitotic M phaseS644
present during mitotic M phaseS947
present during mitotic M phaseS525
present during mitotic M phaseS327
present during mitotic M phaseS361
present during mitotic M phaseS326
present during mitotic M phaseS134
present during mitotic M phaseS885
present during mitotic M phaseS305
present during mitotic M phaseS296
S326, S327, S525, S943, S947
level fluctuates during mitotic cell cycleS361
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S475 PMID:21712547
present during mitotic M phase experimental evidence S943 PMID:21712547
present during mitotic M phase experimental evidence S295 PMID:21712547
present during mitotic M phase experimental evidence S644 PMID:21712547
present during mitotic M phase experimental evidence S947 PMID:21712547
present during mitotic M phase experimental evidence S525 PMID:21712547
present during mitotic M phase experimental evidence S327 PMID:21712547
present during mitotic M phase experimental evidence S361 PMID:21712547
present during mitotic M phase experimental evidence S326 PMID:21712547
present during mitotic M phase experimental evidence S134 PMID:21712547
present during mitotic M phase experimental evidence S885 PMID:21712547
present during mitotic M phase experimental evidence S305 PMID:21712547
present during mitotic M phase experimental evidence S296 PMID:21712547
experimental evidence S947 PMID:24763107
experimental evidence S943 PMID:24763107
experimental evidence S326 PMID:24763107
experimental evidence S525 PMID:24763107
level fluctuates during mitotic cell cycle experimental evidence S361 PMID:24763107
experimental evidence S327 PMID:24763107
O-phospho-L-threonine 700
present during mitotic M phaseT366
present during mitotic M phaseT357
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T366 PMID:21712547
present during mitotic M phase experimental evidence T357 PMID:21712547
ubiquitinylated lysineK159 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K159 PMID:26412298
O-phosphorylated residueS75, S156, S171, S361, S497, S947, T61, T71 1756
Annotation ExtensionEvidenceResidueReference
IDA S497 PMID:23297348
IDA S171 PMID:23297348
IDA S156 PMID:23297348
IDA S947 PMID:23297348
IDA S75 PMID:23297348
IDA T71 PMID:23297348
IDA S361 PMID:23297348
IDA T61 PMID:23297348
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pic1 (SPBC336.15)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
521.54during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
337during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.89during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure may be incorrect15
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC336.15 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
binds DNA-binding domain construct withark1aurora-B kinase Ark1 Two-hybridPMID:11950927
affinity capturesbir1survivin, Bir1 Affinity Capture-WesternPMID:17301288
affinity capturesark1aurora-B kinase Ark1 Affinity Capture-WesternPMID:17301288
modified bylkh1dual specificity protein kinase Lkh1 Biochemical ActivityPMID:26167880
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC336.15 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofark1aurora-B kinase Ark1 Dosage RescuePMID:11950927
synthetic lethal withmde4microtubule-site clamp monopolin complex subunit Mde4 Synthetic LethalityPMID:22264609
synthetic lethal withswi6HP1 family chromodomain protein Swi6 Synthetic LethalityPMID:22264609
synthetic growth defect withswi6HP1 family chromodomain protein Swi6 Synthetic Growth DefectPMID:17322402
overexpression rescuesbir1survivin, Bir1 Dosage RescuePMID:16199877
External References
Database Identifier Description
NBRP SPBC336.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.15 BioGRID Interaction Datasets
Expression Viewer SPBC336.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.15 Transcriptome Viewer (Bähler Lab)
GEO SPBC336.15 GEO profiles
PInt SPBC336.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC336.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC336.15 Fission yeast phenotypic data & analysis
Cyclebase SPBC336.15.1 Cell Cycle Data
SPD / RIKEN47/47G12Orfeome Localization Data
UniProtKB/SwissProtQ1MTN3Inner centromere protein-related protein pic1
ModBaseQ1MTN3Database of comparative protein structure models
STRINGQ1MTN3Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596135INCENP, Pic1
RefSeq mRNANM_001022053972h- INCENP, Pic1 (pic1), mRNA

Literature for pic1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016