pic1 (SPBC336.15)


Gene Standard Namepic1 Characterisation Statuspublished
Systematic IDSPBC336.15 Feature Typeprotein coding
SynonymsSPBC685.01 Name Description
ProductINCENP, Pic1 Product Size1018aa, 114.48 kDa
Genomic Location Chromosome II, 2769046-2772538 (3493nt); CDS:2769271-2772370 (3100nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPIark1PMID:11950927861
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000281mitotic cytokinesisIGIark1PMID:1195092768
GO:0000070mitotic sister chromatid segregationIGIbir1PMID:16199877130
IMPbir1PMID:16199877
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032133chromosome passenger complexNASGO_REF:00000014
GO:0000941condensed nuclear chromosome inner kinetochoreTASPMID:1195092711
GO:0005634nucleusIDAPMID:168233722740
GO:0005819spindleIDAPMID:16823372275
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopypic1ΔNullPECO:0000005, PECO:0000137PMID:236978061315
Microscopypic1ΔNullPMID:20473289
FYPO:0001103resistance to hydrogen peroxide60
expressivity FYPO_EXT:0000001Cell growth assaypic1ΔNullPECO:0000137PMID:19672306
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000002cell growth assaypic1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000097sensitive to caffeine98
expressivity FYPO_EXT:0000001Cell growth assaypic1ΔNullPECO:0000137PMID:19672306
FYPO:0000087sensitive to hydrogen peroxide121
expressivity FYPO_EXT:0000003cell growth assaypic1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopypic1ΔNullPECO:0000005, PECO:0000137PMID:23697806624
FYPO:0000314inviable after spore germination with elongated germ tube162
penetrance FYPO_EXT:0000001Microscopypic1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
127690462769428
227694722772538

UTRs

Region Coordinates Reference
five_prime_UTR2769046..2769270PMID:21511999
three_prime_UTR2772371..2772538PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03941 Pfam IPR005635 Inner centromere protein, ARK-binding domain 921 980 1
PTHR13142:SF1 HMMPANTHER 1 1018 1
PTHR13142 HMMPANTHER 1 1018 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.45 Da
Charge 44.50
Isoelectric point 10.22
Molecular weight 114.48 kDa
Number of residues 1018
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS361PMID:247631071663
level fluctuates during mitotic cell cycle
present during mitotic M phaseS475PMID:21712547
present during mitotic M phaseS327PMID:21712547
present during mitotic M phaseS525PMID:21712547
S327PMID:24763107
S326PMID:24763107
present during mitotic M phaseS644PMID:21712547
S525PMID:24763107
present during mitotic M phaseS305PMID:21712547
present during mitotic M phaseS134PMID:21712547
present during mitotic M phaseS947PMID:21712547
present during mitotic M phaseS295PMID:21712547
present during mitotic M phaseS943PMID:21712547
present during mitotic M phaseS296PMID:21712547
S947PMID:24763107
present during mitotic M phaseS326PMID:21712547
S943PMID:24763107
present during mitotic M phaseS885PMID:21712547
present during mitotic M phaseS361PMID:21712547
MOD:00047O-phospho-L-threonineT366PMID:21712547682
present during mitotic M phase
present during mitotic M phaseT357PMID:21712547
MOD:01455O-phosphorylated residueIDAS361PMID:232973481742
IDAS171PMID:23297348
IDAS156PMID:23297348
IDAS75PMID:23297348
IDAT71PMID:23297348
IDAT61PMID:23297348
IDAS497PMID:23297348
IDAS947PMID:23297348
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
337during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
521.54during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.2during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.89during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure may be incorrect15
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
swi6HP1 family chromodomain protein Swi6 Synthetic Growth DefectPMID:17322402
Synthetic LethalityPMID:22264609
mde4monopolin-like complex subunit Mde4 Synthetic LethalityPMID:22264609
bir1survivin, Bir1 Dosage RescuePMID:16199877
ark1aurora-B kinase Ark1 Dosage RescuePMID:11950927
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
ark1aurora-B kinase Ark1 Affinity Capture-WesternPMID:17301288
Two-hybridPMID:11950927
bir1survivin, Bir1 Affinity Capture-WesternPMID:17301288
External References
Database Identifier Description
NBRP SPBC336.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.15 BioGRID Interaction Datasets
Expression Viewer SPBC336.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.15 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC336.15 Cell Cycle Data
GEO SPBC336.15 GEO profiles
PInt SPBC336.15 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2540250INCENP, Pic1
WikiGene2540250INCENP, Pic1
SPD / RIKEN47/47G12Orfeome Localization Data
UniProtKB/SwissProtQ1MTN3Inner centromere protein-related protein pic1
ModBaseQ1MTN3Database of comparative protein structure models
STRINGQ1MTN3Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596135INCENP, Pic1
RefSeq mRNANM_001022053972h- INCENP, Pic1 (pic1), mRNA
European Nucleotide ArchiveCAB58167ENA Protein Mapping
European Nucleotide ArchiveCAB58167.1ENA Protein Mapping
UniParcUPI000006C889UniProt Archive

Literature for pic1

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014