pic1 (SPBC336.15)


Gene Standard Namepic1 Characterisation Statuspublished
Systematic IDSPBC336.15 Feature Typeprotein coding
SynonymsSPBC685.01 Name Description
ProductINCENP, Pic1 Product Size1018aa, 114.48 kDa
Genomic Location Chromosome II, 2769046-2772538 (3493nt); CDS:2769271-2772370 (3100nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding859
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic cytokinesis110
Annotation ExtensionEvidenceWith/FromReference
mitotic sister chromatid segregation184
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
chromosome passenger complex4
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome inner kinetochore10
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle259
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationpic1ΔNull1438
resistance to hydrogen peroxidepic1ΔNull73
sensitive to cadmiumpic1ΔNull246
sensitive to caffeine during vegetative growthpic1ΔNull106
sensitive to hydrogen peroxidepic1ΔNull139

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclepic1ΔNull834
inviable after spore germination with elongated germ tubepic1ΔNull164
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2769046..2769428, 2769472..2772538
mRNA2769046..2772538
5' UTR2769046..2769270PMID:21511999
CDS2769271..2769428, 2769472..2772370
3' UTR2772371..2772538PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03941 Pfam IPR005635 Inner centromere protein, ARK-binding domain 921 980 1
PTHR13142 HMMPANTHER 1 1018 1
PTHR13142:SF1 HMMPANTHER 1 1018 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.45 Da
Charge 44.50
Isoelectric point 10.22
Molecular weight 114.48 kDa
Number of residues 1018
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS295
S326, S327, S525, S943, S947
present during mitotic M phaseS885
present during mitotic M phaseS134
present during mitotic M phaseS947
present during mitotic M phaseS943
present during mitotic M phaseS326
present during mitotic M phaseS296
present during mitotic M phaseS361
present during mitotic M phaseS525
present during mitotic M phaseS327
present during mitotic M phaseS475
level fluctuates during mitotic cell cycleS361
present during mitotic M phaseS305
present during mitotic M phaseS644
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S134 PMID:21712547
present during mitotic M phase experimental evidence S295 PMID:21712547
present during mitotic M phase experimental evidence S296 PMID:21712547
present during mitotic M phase experimental evidence S305 PMID:21712547
experimental evidence S326 PMID:24763107
present during mitotic M phase experimental evidence S326 PMID:21712547
experimental evidence S327 PMID:24763107
present during mitotic M phase experimental evidence S327 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S361 PMID:24763107
present during mitotic M phase experimental evidence S361 PMID:21712547
present during mitotic M phase experimental evidence S475 PMID:21712547
experimental evidence S525 PMID:24763107
present during mitotic M phase experimental evidence S525 PMID:21712547
present during mitotic M phase experimental evidence S644 PMID:21712547
present during mitotic M phase experimental evidence S885 PMID:21712547
experimental evidence S943 PMID:24763107
present during mitotic M phase experimental evidence S943 PMID:21712547
experimental evidence S947 PMID:24763107
present during mitotic M phase experimental evidence S947 PMID:21712547
O-phospho-L-threonine 698
present during mitotic M phaseT366
present during mitotic M phaseT357
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T357 PMID:21712547
present during mitotic M phase experimental evidence T366 PMID:21712547
O-phosphorylated residueS75, S156, S171, S361, S497, S947, T61, T71 1754
Annotation ExtensionEvidenceResidueReference
IDA S75 PMID:23297348
IDA S156 PMID:23297348
IDA S171 PMID:23297348
IDA S361 PMID:23297348
IDA S497 PMID:23297348
IDA S947 PMID:23297348
IDA T61 PMID:23297348
IDA T71 PMID:23297348
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
521.54during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
337during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.89during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure may be incorrect15
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesark1aurora-B kinase Ark1 Affinity Capture-WesternPMID:17301288
binds DNA-binding domain construct withark1aurora-B kinase Ark1 Two-hybridPMID:11950927
affinity capturesbir1survivin, Bir1 Affinity Capture-WesternPMID:17301288
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofark1aurora-B kinase Ark1 Dosage RescuePMID:11950927
overexpression rescuesbir1survivin, Bir1 Dosage RescuePMID:16199877
synthetic lethal withmde4microtubule-site clamp monopolin complex subunit Mde4 Synthetic LethalityPMID:22264609
synthetic growth defect withswi6HP1 family chromodomain protein Swi6 Synthetic Growth DefectPMID:17322402
synthetic lethal withswi6HP1 family chromodomain protein Swi6 Synthetic LethalityPMID:22264609
External References
Database Identifier Description
NBRP SPBC336.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC336.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC336.15 BioGRID Interaction Datasets
Expression Viewer SPBC336.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC336.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC336.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC336.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC336.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC336.15 Transcriptome Viewer (Bähler Lab)
GEO SPBC336.15 GEO profiles
PInt SPBC336.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC336.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC336.15 Fission yeast phenotypic data & analysis
Cyclebase SPBC336.15.1 Cell Cycle Data
SPD / RIKEN47/47G12Orfeome Localization Data
UniProtKB/SwissProtQ1MTN3Inner centromere protein-related protein pic1
ModBaseQ1MTN3Database of comparative protein structure models
STRINGQ1MTN3Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596135INCENP, Pic1
RefSeq mRNANM_001022053972h- INCENP, Pic1 (pic1), mRNA
European Nucleotide ArchiveCAB58167.1ENA Protein Mapping
UniParcUPI000006C889UniProt Archive

Literature for pic1

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015