crb2 (SPBC342.05)


Gene Standard Namecrb2 Characterisation Statuspublished
Systematic IDSPBC342.05 Feature Typeprotein coding
Synonymsrhp9 Name Description
ProductDNA repair protein Rad9 homolog, Rhp9 Product Size778aa, 87.46 kDa
Genomic Location Chromosome II, 4210933-4213577 (2645nt); CDS:4211087-4213423 (2337nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPIrad4PMID:24074952865
IPIcrb2PMID:24074952
IPIchk1PMID:9407031
IPIrad4PMID:9407031
active form PR:000037503IPIchk1PMID:22792081
IPIrad3PMID:14739927
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0007095mitotic G2 DNA damage checkpointIMPPMID:915331312
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000790nuclear chromatinISMPFAM:PF00533GO_REF:0000001278
GO:0005634nucleusIDAPMID:168233722737
IDAPMID:24074952
GO:0035861site of double-strand breakIDAPMID:2362848146
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000969normal growth during cellular response to UVCell growth assaycrb2-8AQ (S18A, S89A, S145A, S170A, S305A, S353A, S512A, S772A)EndogenousPECO:0000005, PECO:0000102, PECO:0000137PMID:2279208126
Cell growth assayV188P (V188P)Not specifiedPECO:0000102PMID:24074952
FYPO:0002169normal growth during cellular response to gamma radiationCell growth assayV188P (V188P)Not specifiedPECO:0000102PMID:240749527
FYPO:0001690normal growth on camptothecinCell growth assaycrb2-8AQ (S18A, S89A, S145A, S170A, S305A, S353A, S512A, S772A)EndogenousPECO:0000102, PECO:0000137, PECO:0000005PMID:2279208115
FYPO:0000963normal growth on hydroxyureaCell growth assaycrb2-8AQ (S18A, S89A, S145A, S170A, S305A, S353A, S512A, S772A)EndogenousPECO:0000005, PECO:0000102, PECO:0000137PMID:2279208121
FYPO:0001357normal vegetative cell population growthCell growth assayT187A (T187A)Not specifiedPECO:0000137, PECO:0000102, PECO:0000005PMID:24074952618
Cell growth assayT215A (T215A)Not specifiedPECO:0000137, PECO:0000102, PECO:0000005PMID:24074952
Cell growth assaycrb2ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:24074952
Cell growth assayT235A (T235A)Not specifiedPECO:0000137, PECO:0000102, PECO:0000005PMID:24074952
FYPO:0001098sensitive to 4-nitroquinoline N-oxide159
expressivity FYPO_EXT:0000002Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0002550sensitive to UVCell growth assaycrb2-2AQ (T73A, S80A)EndogenousPECO:0000102, PECO:0000137PMID:22792081121
FYPO:0000268sensitive to UV during vegetative growthCell growth assayT187A (T187A)Not specifiedPECO:0000137, PECO:0000102, PECO:0000005PMID:24074952120
Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000102PMID:24806815
competitive growth assay evidencecrb2ΔNullPMID:20537132
Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000005PMID:23173672
Cell growth assayV184K (V184K)Not specifiedPECO:0000102PMID:24074952
Cell growth assayT215A (T215A)Not specifiedPECO:0000137, PECO:0000102, PECO:0000005PMID:24074952
Cell growth assayV188P,T215A,T235A (V188P, T215A, T235A)Not specifiedPECO:0000102PMID:24074952
Cell growth assayT215A,T235A (T215A, T235A)Not specifiedPECO:0000102PMID:24074952
Cell growth assayT235A (T235A)Not specifiedPECO:0000137, PECO:0000102, PECO:0000005PMID:24074952
Cell growth assaycrb2ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:24074952
FYPO:0000095sensitive to bleomycinCell growth assaycrb2ΔNullPECO:0000137, PECO:0000005PMID:2317367247
FYPO:0000085sensitive to camptothecinCell growth assaycrb2-2AQ (T73A, S80A)EndogenousPECO:0000102, PECO:0000137PMID:22792081211
Cell growth assaycrb2ΔNullPECO:0000102PMID:24074952
Cell growth assayF400A (F400A)Not specifiedPECO:0000137, PECO:0000102PMID:24806815
Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000102PMID:24806815
expressivity FYPO_EXT:0000003Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0000088sensitive to hydroxyurea513
expressivity FYPO_EXT:0000003Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000005PMID:19264558
Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000102PMID:24806815
Cell growth assaycrb2ΔNullPECO:0000102PMID:24074952
Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000005PMID:23173672
expressivity FYPO_EXT:0000002Cell Growth Assaycrb2ΔNullPECO:0000137, PECO:0000102PMID:23697806
Cell growth assaycrb2-2AQ (T73A, S80A)EndogenousPECO:0000102, PECO:0000137PMID:22792081
FYPO:0000267sensitive to ionizing radiationCell growth assayT215A (T215A)Not specifiedPECO:0000137, PECO:0000102, PECO:0000005PMID:2407495244
Cell growth assaycrb2ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:24074952
Cell growth assaycrb2-2AQ (T73A, S80A)EndogenousPECO:0000102, PECO:0000137PMID:22792081
Cell growth assayV188P,T215A,T235A (V188P, T215A, T235A)Not specifiedPECO:0000102PMID:24074952
Cell growth assayT215A,T235A (T215A, T235A)Not specifiedPECO:0000102PMID:24074952
Cell growth assayT235A (T235A)Not specifiedPECO:0000137, PECO:0000102, PECO:0000005PMID:24074952
Cell growth assayT187A (T187A)Not specifiedPECO:0000137, PECO:0000102, PECO:0000005PMID:24074952
Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000102PMID:24806815
expressivity FYPO_EXT:0000003Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0000089sensitive to methyl methanesulfonate214
expressivity FYPO_EXT:0000003Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000005PMID:19264558
Cell growth assaycrb2ΔNullPECO:0000102PMID:24074952
Cell growth assaycrb2ΔNullPECO:0000137, PECO:0000005PMID:23173672
FYPO:0002060viable vegetative cell populationMicroscopycrb2ΔNullPECO:0000005, PECO:0000137PMID:236978063751
Microscopynot recorded (unrecorded)PMID:9153313
Microscopycrb2ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003489abnormal cell cycle regulation during cellular response to ionizing radiationMicroscopycrb2ΔNullPECO:0000004PMID:227920816
Microscopycrb2-2AQ (T73A, S80A)EndogenousPECO:0000004PMID:22792081
FYPO:0000705abolished protein binding56
affecting rad4 and crb2OtherV184K (V184K)Not specifiedPECO:0000140PMID:24074952
affecting rad4 and crb2OtherV184G (V184G)Not specifiedPECO:0000140PMID:24074952
FYPO:0003486abolished protein localization to double-strand break site4
affecting chk1Microscopycrb2-2AQ (T73A, S80A)EndogenousPECO:0000126, PECO:0000005PMID:22792081
affecting chk1Microscopycrb2ΔNullPECO:0000005, PECO:0000126PMID:22792081
affecting chk1Microscopycrb2(276-778) (1-275)EndogenousPECO:0000126, PECO:0000005PMID:22792081
FYPO:0003130advanced mitotic G1 phase entry during cellular response to ionizing radiationMicroscopycrb2-2AQ (T73A, S80A)EndogenousPMID:227920812
MicroscopyT187A (T187A)Not specifiedPMID:24074952
MicroscopyT235A (T235A)Not specifiedPMID:24074952
MicroscopyT215A (T215A)Not specifiedPMID:24074952
FYPO:0001270complete but unequal mitotic sister chromatid separationMicroscopycrb2ΔNullPECO:0000004PMID:227920817
Microscopycrb2-2AQ (T73A, S80A)EndogenousPECO:0000004PMID:22792081
FYPO:0003487cut during cellular response to ionizing radiation1
penetrance FYPO_EXT:0000002Microscopycrb2ΔNullPECO:0000004PMID:22792081
penetrance FYPO_EXT:0000002Microscopycrb2-2AQ (T73A, S80A)EndogenousPECO:0000004PMID:22792081
FYPO:0002474decreased protein localization to double-strand break site4
affecting chk1Microscopycrb2-S80A (S80A)EndogenousPECO:0000126, PECO:0000005PMID:22792081
affecting chk1Microscopycrb2-T73A (T73A)EndogenousPECO:0000126, PECO:0000005PMID:22792081
FYPO:0002897decreased protein phosphorylation during cellular response to DNA damage3
affecting chk1Western blot assaycrb2-2AQ (T73A, S80A)EndogenousPMID:22792081
FYPO:0002647decreased protein threonine phosphorylation during mitotic M phase2
affecting PR:000037316Western blot assayT215A (T215A)Not specifiedPMID:24074952
affecting PR:000037316Western blot assayT235A (T235A)Not specifiedPMID:24074952
FYPO:0003488elongated cell during cellular response to ionizing radiationMicroscopycrb2-T73A (T73A)EndogenousPECO:0000005PMID:227920811
Microscopycrb2-S80A (S80A)EndogenousPECO:0000005PMID:22792081
FYPO:0003584increased double-strand break repair via nonhomologous end joiningOthercrb2ΔNullPMID:103735823
FYPO:0003129loss of punctate nuclear protein localization during cellular response to ionizing radiation3
affecting chk1Microscopycrb2-2AQ (T73A, S80A)EndogenousPMID:22792081
affecting crb2MicroscopyT215A (T215A)Not specifiedPMID:24074952
affecting crb2MicroscopyV184K (V184K)Not specifiedPMID:24074952
affecting crb2MicroscopyT187A (T187A)Not specifiedPMID:24074952
affecting crb2MicroscopyT235A (T235A)Not specifiedPMID:24074952
FYPO:0001383normal DNA contentFlow cytometry datacrb2ΔNullPECO:0000137, PECO:0000005PMID:2317367219
FYPO:0000703normal protein binding35
affecting rad4 and crb2OtherV188P (V188P)Not specifiedPECO:0000140PMID:24074952
FYPO:0002554normal protein localization to site of double-strand break2
affecting crb2Microscopycrb2-2AQ (T73A, S80A)EndogenousPECO:0000126, PECO:0000005PMID:22792081
affecting crb2Microscopycrb2-T73A (T73A)EndogenousPECO:0000005, PECO:0000126PMID:22792081
affecting crb2Microscopycrb2-S80A (S80A)EndogenousPECO:0000126, PECO:0000005PMID:22792081
affecting chk1Microscopycrb2(1358)-LZ (del aa 359-778 with C-terminal heterologous leucine zipper)EndogenousPECO:0000126, PECO:0000005PMID:22792081
FYPO:0003132normal punctate nuclear localization during cellular response to ionizing radiation2
affecting crb2MicroscopyS234A (S234A)Not specifiedPMID:24074952
affecting crb2Microscopy1-358 (359-778)Not specifiedPMID:24074952
affecting crb2Microscopycrb2-2AQ (T73A, S80A)EndogenousPMID:22792081
FYPO:0002239shortened telomeresGel electrophoresis evidencecrb2ΔNullPMID:1037358229
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopycrb2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in crb2 DNA repair protein Rad9 homolog, Rhp9 PMID:24074952
FYPO affected by mutation in crb2 DNA repair protein Rad9 homolog, Rhp9 PMID:22792081
FYPO affected by mutation in dbl1 Schizosaccharomyces specific protein, double strand break localizing Dbl1 PMID:23628481
FYPO affected by mutation in rad4 BRCT domain protein Rad4 PMID:24074952
GO localized by set9 histone lysine H3-K20 methyltransferase Set9 PMID:15550243
GO substrate of cdc2 cyclin-dependent protein kinase Cdk1/Cdc2 PMID:24074952
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
142109334213577
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00533 Pfam IPR001357 BRCT domain 536 640 8
SM00292 SMART IPR001357 BRCT domain 537 643 10
PS50172 Prosite Profiles IPR001357 BRCT domain 535 653 13
PS50172 Prosite Profiles IPR001357 BRCT domain 759 778 13
PTHR15321 HMMPANTHER 8 777 1
3.40.50.10190 Gene3D IPR001357 BRCT domain 536 654 13
SSF52113 SuperFamily IPR001357 BRCT domain 534 669 12

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000443BRCT domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000443
PBO:0001140tudor domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001140

Protein Properties

Ave. residue weight 112.42 Da
Charge 11.50
Isoelectric point 8.26
Molecular weight 87.46 kDa
Number of residues 778
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS80PMID:227920811670
present during mitotic M phaseexperimental evidenceS89PMID:21712547
present during mitotic M phaseexperimental evidenceS88PMID:21712547
present during mitotic M phaseexperimental evidenceS93PMID:21712547
experimental evidenceS93PMID:24763107
experimental evidenceS89PMID:24763107
present during mitotic M phaseexperimental evidenceS47PMID:21712547
present during mitotic M phaseexperimental evidenceS43PMID:21712547
MOD:00047O-phospho-L-threonineIDAT235PMID:24074952690
IDAT73PMID:22792081
IDAT215PMID:24074952
IDAT187PMID:24074952
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.6during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.65during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer142
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
apc10anaphase-promoting complex subunit Apc10 Negative GeneticPMID:22681890
slx1structure-specific endonuclease catalytic subunit Positive GeneticPMID:22681890
cdc24DNA replication protein Cdc24 Phenotypic EnhancementPMID:9928931
taz1human TRF ortholog Taz1 Phenotypic SuppressionPMID:12196391
rad9checkpoint clamp complex protein Rad9 Dosage RescuePMID:12839619
pol1DNA polymerase alpha catalytic subunit Phenotypic EnhancementPMID:9928931
Synthetic Lethality
Phenotypic EnhancementPMID:14739927
Phenotypic SuppressionPMID:9407031
rem1meiosis-specific cyclin Rem1 Negative GeneticPMID:22681890
cdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:9928931
Phenotypic EnhancementPMID:14739927
Phenotypic EnhancementPMID:18667534
Phenotypic SuppressionPMID:9407031
SPAC1F12.04cconserved fungal protein Positive GeneticPMID:22681890
rad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
cdc18MCM loader Phenotypic EnhancementPMID:9928931
fml2ATP-dependent 3' to 5' DNA helicase (predicted) Negative GeneticPMID:22681890
swi10DNA repair endonuclease Swi10 Synthetic Growth DefectPMID:18931302
cdc27DNA polymerase delta subunit Cdc27 Synthetic LethalityPMID:9928931
Phenotypic Enhancement
rad57RecA family ATPase Rad57/Rhp57 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
mus81Holliday junction resolvase subunit Mus81 Synthetic Growth DefectPMID:12589755
rhp7Rad7 homolog Rhp7 Negative GeneticPMID:18818364
mcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:18818364
rad17RFC related checkpoint protein Rad17 Dosage RescuePMID:12839619
orc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
rqh1RecQ type DNA helicase Rqh1 Synthetic Growth DefectPMID:12589755
Synthetic RescuePMID:12023299
Synthetic Growth DefectPMID:10373582
emc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
rad51RecA family recombinase Rad51/Rhp51 Synthetic Growth DefectPMID:12839619
Dosage Rescue
Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Positive GeneticPMID:22681890
SPAC750.08cNAD-dependent malic enzyme (predicted), partial Negative GeneticPMID:22681890
pcn1PCNA Phenotypic EnhancementPMID:9928931
SPAC227.17cconserved protein Positive GeneticPMID:22681890
pof3F-box protein Pof3 Synthetic LethalityPMID:11809834
Synthetic Growth DefectPMID:18931302
prz1calcineurin responsive transcription factor Prz1 Positive GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Dosage RescuePMID:12839619
rsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
tlg2SNARE Tlg2 (predicted) Synthetic Growth DefectPMID:18931302
sum2G2/M transition checkpoint protein Sum2 Positive GeneticPMID:22681890
ams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
rad4BRCT domain protein Rad4 Synthetic Growth DefectPMID:16778077
Phenotypic EnhancementPMID:24074952
Dosage RescuePMID:9407031
ppk31serine/threonine protein kinase Ppk31 (predicted) Positive GeneticPMID:18818364
exo1exonuclease I Exo1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
pku70Ku domain protein Pku70 Phenotypic SuppressionPMID:12196391
cdc6DNA polymerase delta catalytic subunit Cdc6 Synthetic LethalityPMID:12589755
Phenotypic EnhancementPMID:14739927
Phenotypic SuppressionPMID:9407031
brc1BRCT domain protein Brc1 Negative GeneticPMID:20094029
SPAC186.09pyruvate decarboxylase (predicted) Negative GeneticPMID:22681890
fml1ATP-dependent 3' to 5' DNA helicase, FANCM ortholog Fml1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
SPCC594.06cvacuolar SNARE Vam7 (predicted) Positive GeneticPMID:22681890
ctp1CtIP-related endonuclease Phenotypic EnhancementPMID:21098122
Synthetic Growth Defect
cut2securin, sister chromatid separation inhibitor Synthetic Growth DefectPMID:15329725
cut1separase/separin Synthetic Growth DefectPMID:9153313
rad52DNA recombination protein Rad52 (previously Rad22) Synthetic LethalityPMID:10373582
hta2histone H2A beta Synthetic Growth DefectPMID:20679485
Phenotypic SuppressionPMID:16314498
Phenotypic Enhancement
Synthetic RescuePMID:16778077
Synthetic Growth Defect
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Synthetic Growth DefectPMID:12839619
dcd1deoxycytidylate deaminase (predicted) Negative GeneticPMID:22681890
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic Growth DefectPMID:20176980
vps38phophatidylinositol 3-kinase complex subunit Vps38 Negative GeneticPMID:22681890
yps1aspartic protease, yapsin Yps1 Negative GeneticPMID:22681890
pet801mitochondrial S-adenosylmethionine transporter (predicted) Positive GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
bdf2BET family double bromodomain protein Bdf2 Synthetic RescuePMID:22095079
SPAC212.04cS. pombe specific DUF999 family protein 1 Negative GeneticPMID:22681890
hst4Sir2 family histone deacetylase Hst4 Synthetic LethalityPMID:18344406
mdb1BRCT domain protein Mdb1 Synthetic Growth DefectPMID:24806815
bdf1Swr1 complex bromodomain subunit Brf1 Synthetic RescuePMID:22095079
ayr11-acyldihydroxyacetone phosphate reductase (predicted) Positive GeneticPMID:22681890
hta1histone H2A alpha Synthetic Growth DefectPMID:20679485
Phenotypic SuppressionPMID:16314498
Phenotypic Enhancement
Synthetic RescuePMID:16778077
Synthetic Growth Defect
set1histone lysine methyltransferase Set1 Synthetic RescuePMID:12589755
top3DNA topoisomerase III Dosage RescuePMID:12023299
wee1M phase inhibitor protein kinase Wee1 Synthetic Growth DefectPMID:20679488
swi1replication fork protection complex subunit Swi1 Synthetic Growth DefectPMID:14560029
rad1checkpoint clamp complex protein Rad1 Dosage RescuePMID:12839619
chk1Chk1 protein kinase Phenotypic SuppressionPMID:12081644
Synthetic RescuePMID:16816416
Synthetic RescuePMID:15229228
Dosage RescuePMID:9407031
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
SPBC1348.03S. pombe specific 5Tm protein family Negative GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
lsg1Lsk1 complex gamma subunit (predicted) Negative GeneticPMID:22681890
srs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
SPBC3H7.05cmitochondrial Membrane Bound O-Acyl Transferase (MBOAT) family Positive GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
rhp41DNA repair protein Rhp41 Synthetic Growth DefectPMID:18931302
csn2COP9/signalosome complex subunit Csn2 Negative GeneticPMID:22681890
spp1DNA primase catalytic subunit Spp1 Synthetic RescuePMID:12589755
cdc10MBF transcription factor complex subunit Cdc10 Phenotypic EnhancementPMID:9928931
sdu1PPPDE peptidase family (predicted) Positive GeneticPMID:22681890
mrc1mediator of replication checkpoint 1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
rad13DNA repair nuclease Rad13 Synthetic Growth DefectPMID:9153313
grx3monothiol glutaredoxin Grx3 Negative GeneticPMID:22681890
swd2Set1C complex subunit Swd2.1 Synthetic RescuePMID:12589755
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
cdc17ATP-dependent DNA replication ligase Cdc17 Phenotypic EnhancementPMID:9928931
set9histone lysine H3-K20 methyltransferase Set9 Synthetic Growth DefectPMID:16778077
Synthetic Growth DefectPMID:15550243
SPBC16H5.12cconserved fungal protein Negative GeneticPMID:22681890
erd1Erd1 homolog (predicted) Positive GeneticPMID:22681890
SPBC725.04oxalyl-CoA decarboxylase (predicted) Positive GeneticPMID:22681890
cut14condensin complex subunit Cut14 Synthetic Growth DefectPMID:22645654
SPCC1020.05phosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
hrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
rad3ATR checkpoint kinase Rad3 Synthetic Growth DefectPMID:21098122
Phenotypic Enhancement
stm1G-protein coupled receptor Stm1 Positive GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
rhp23Rad23 homolog Rhp23 Negative GeneticPMID:22681890
spo15sporulation protein Spo15 Positive GeneticPMID:22681890
rhp14XP-A family homolog Rhp14 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
rad2FEN-1 endonuclease Rad2 Synthetic Growth DefectPMID:18931302
Synthetic Growth DefectPMID:9153313
mre11Mre11 nuclease Negative GeneticPMID:18818364
ptp4phosphatidate cytidylyltransferase Ptp4 (predicted) Negative GeneticPMID:22681890
SPAC2H10.02c26S proteasome regulatory particle assembly protein (predicted) Positive GeneticPMID:22681890
SPAC31A2.12arrestin/PY protein 1 (predicted) Negative GeneticPMID:22681890
swi3replication fork protection complex subunit Swi3 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
rps40140S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rad4BRCT domain protein Rad4 Co-crystal or NMR structurePMID:24074952
Affinity Capture-Western
Two-hybridPMID:9407031
Reconstituted Complex
Two-hybridPMID:16778077
Two-hybridPMID:15229228
Affinity Capture-WesternPMID:24663817
Reconstituted Complex
ptc1protein phosphatase 2C Ptc1 Two-hybridPMID:23695164
sad1spindle pole body protein Sad1 Two-hybridPMID:14655046
chk1Chk1 protein kinase Affinity Capture-WesternPMID:23422000
Two-hybridPMID:9407031
Two-hybridPMID:15229228
Affinity Capture-WesternPMID:14739927
Affinity Capture-WesternPMID:22792081
Reconstituted Complex
Protein-peptide
hta2histone H2A beta Affinity Capture-WesternPMID:20679488
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:24074952
Biochemical ActivityPMID:10488332
rad3ATR checkpoint kinase Rad3 Affinity Capture-WesternPMID:14739927
cdc13G2/M B-type cyclin Cdc13 Two-hybridPMID:23695164
hta1histone H2A alpha Affinity Capture-WesternPMID:20679488
rad9checkpoint clamp complex protein Rad9 Affinity Capture-WesternPMID:24663817
hhf1histone H4 h4.1 Reconstituted ComplexPMID:18826944
set9histone lysine H3-K20 methyltransferase Set9 Biochemical ActivityPMID:15550243
crb2DNA repair protein Rad9 homolog, Rhp9 Reconstituted ComplexPMID:18676809
Affinity Capture-WesternPMID:15229228
Two-hybrid
External References
Database Identifier Description
NBRP SPBC342.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC342.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC342.05 BioGRID Interaction Datasets
Expression Viewer SPBC342.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC342.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC342.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC342.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC342.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC342.05 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC342.05 Cell Cycle Data
GEO SPBC342.05 GEO profiles
PInt SPBC342.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC342.05 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN33/33G08Orfeome Localization Data
UniProtKB/SwissProtP87074DNA repair protein rhp9
ModBaseP87074Database of comparative protein structure models
STRINGP87074Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596748DNA repair protein
RefSeq mRNANM_001023768972h- DNA repair protein (crb2), mRNA
ePDB2FHDThe European PDB
PDB2FHDPDB
PDBsum2FHDPDBsum
ePDB2VXBThe European PDB
PDB2VXBPDB
PDBsum2VXBPDBsum
ePDB2VXCThe European PDB
PDB2VXCPDB
PDBsum2VXCPDBsum
ePDB4BU0The European PDB
PDB4BU0PDB
PDBsum4BU0PDBsum
ePDB4BU1The European PDB
PDB4BU1PDB
PDBsum4BU1PDBsum
European Nucleotide ArchiveBAA13093.1ENA Protein Mapping
European Nucleotide ArchiveCAA70582.1ENA Protein Mapping
European Nucleotide ArchiveCAB46775.1ENA Protein Mapping
UniParcUPI00001338AAUniProt Archive

Literature for crb2

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014