crb2 (SPBC342.05)


Gene Standard Namecrb2 Characterisation Statuspublished
Systematic IDSPBC342.05 Feature Typeprotein coding
Synonymsrhp9 Name Description
ProductDNA repair protein Rad9 homolog Crb2 Product Size778aa, 87.46 kDa
Genomic Location Chromosome II, 4210933-4213577 (2645nt); CDS:4211087-4213423 (2337nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding887
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic DNA replication checkpoint19
Annotation ExtensionEvidenceWith/FromReference
mitotic G2 DNA damage checkpoint7
Annotation ExtensionEvidenceWith/FromReference
negative regulation of protein kinase activity16
Annotation ExtensionEvidenceWith/FromReference
positive regulation of DNA repair9
Annotation ExtensionEvidenceWith/FromReference
regulation of double-strand break repair via homologous recombination7
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in mitotic DNA damage checkpoint8
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear chromatin365
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
site of double-strand break54
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
normal growth during cellular response to gamma radiationV188P (V188P)18
normal growth during cellular response to UVcrb2-BRCT(D754term) (1-534, 754-778)79
V188P (V188P)
crb2-BRCT(S666A) (aa 1-534 deleted, S666A)
crb2-8AQ (S18A, S89A, S145A, S170A, S305A, S353A, S512A, S772A)
normal growth on camptothecincrb2-8AQ (S18A, S89A, S145A, S170A, S305A, S353A, S512A, S772A)43
normal growth on glucose carbon sourcecrb2Δ294
normal growth on hydroxyureacrb2-8AQ (S18A, S89A, S145A, S170A, S305A, S353A, S512A, S772A)75
normal growth on methyl methanesulfonatecrb2-1 (C1789T)58
normal vegetative cell population growthT215A (T215A)813
T187A (T187A)
crb2Δ
T235A (T235A)
sensitive to 4-nitroquinoline N-oxidecrb2Δ159
sensitive to bleomycincrb2Δ92
sensitive to camptothecinF400A (F400A)257
crb2-2AQ (T73A, S80A)
crb2Δ
sensitive to hydroxyureacrb2-2AQ (T73A, S80A)594
crb2Δ
sensitive to ionizing radiation during vegetative growthcrb2-T215A (T215A)74
T215A,T235A (T215A, T235A)
crb2-2AQ (T73A, S80A)
T235A (T235A)
T215A (T215A)
T187A (T187A)
V188P,T215A,T235A (V188P, T215A, T235A)
crb2Δ
sensitive to methyl methanesulfonatecrb2-1 (C1789T)331
crb2Δ
sensitive to UV during vegetative growthcrb2-BRCT(Y651F) (aa 1-534 deleted, Y651F)161
T215A,T235A (T215A, T235A)
crb2-2AQ (T73A, S80A)
V188P,T215A,T235A (V188P, T215A, T235A)
crb2-BRCT(S772A) (aa 1-534 deleted, S772A)
crb2-BRCT (1-534)
T215A (T215A)
V184K (V184K)
Crb2ΔC (547-778)
T187A (T187A)
T235A (T235A)
crb2Δ
viable vegetative cell populationcrb2Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cycle regulation during cellular response to hydroxyureacrb2-1 (C1789T)15
crb2Δ
abnormal mitotic cell cycle regulation during cellular response to ionizing radiationcrb2-2AQ (T73A, S80A)13
crb2Δ
abnormal mitotic cell cycle regulation during cellular response to UVcrb2Δ23
abnormal mitotic DNA damage checkpoint during cellular response to UVCrb2ΔC (547-778)10
abolished protein binding147
affecting rad4 and crb2V184G (V184G)
affecting rad4 and crb2V184K (V184K)
affecting rad3 and crb2 (1-232, 608-778)
affecting rad3 and crb2Crb2ΔC1-607 (608-778)
affecting rad3 and crb2 (299-778)
affecting rad3 and crb2 (1-232)
abolished protein localization to double-strand break site8
affects localization of chk1crb2Δ
affects localization of chk1crb2-2AQ (T73A, S80A)
affects localization of chk1crb2(276-778) (1-275)
abolished protein phosphorylation during cellular response to ionizing radiation6
affecting chk1crb2Δ
complete but unequal mitotic sister chromatid segregationcrb2-2AQ (T73A, S80A)35
crb2Δ
cut during cellular response to ionizing radiationcrb2-2AQ (T73A, S80A)2
crb2Δ
cut during cellular response to methyl methanesulfonatecrb2-1 (C1789T)2
crb2Δ
cut during cellular response to UVCrb2ΔC1-607 (608-778)2
Crb2ΔC (547-778)
decreased negative regulation of protein kinase activity1
affecting activity of rad3crb2-BRCT(S666A) (aa 1-534 deleted, S666A)
decreased protein kinase activity56
affecting activity of rad3crb2-BRCT (1-534)
decreased protein kinase activity during cellular response to UV1
affecting substrate cdc25, affecting activity of chk1Crb2ΔC (547-778)
decreased protein localization to double-strand break site5
affects localization of chk1crb2-T73A (T73A)
affects localization of chk1crb2-S80A (S80A)
decreased protein phosphorylation during cellular response to camptothecin4
affecting rad9crb2Δ
decreased protein phosphorylation during cellular response to DNA damage3
affecting chk1crb2-2AQ (T73A, S80A)
decreased protein phosphorylation during cellular response to UV6
affecting chk1Crb2ΔC (547-778)
affecting chk1crb2Δ
decreased protein threonine phosphorylation during mitotic M phase2
affecting PR:000037316T215A (T215A)
affecting PR:000037316T235A (T235A)
elongated cell during cellular response to ionizing radiationcrb2-S80A (S80A)1
crb2-T73A (T73A)
increased cellular reactive oxygen species level during vegetative growthcrb2Δ28
increased DNA recombination during cellular response to ionizing radiationcrb2-T215A (T215A)2
increased double-strand break repair via nonhomologous end joiningcrb2Δ5
increased number of Rqh1 foci during cellular response to ionizing radiationcrb2-T215A (T215A)2
inviable elongated vegetative cell during cellular response to UVCrb2ΔC (547-778)2
Crb2ΔC1-607 (608-778)
loss of punctate nuclear protein localization during cellular response to ionizing radiation4
affects localization of crb2T215A (T215A)
affects localization of crb2T235A (T235A)
affects localization of crb2V184K (V184K)
affects localization of crb2T187A (T187A)
affects localization of chk1crb2-2AQ (T73A, S80A)
normal cell cycle regulation during cellular response to hydroxyureacrb2Δ20
crb2-1 (C1789T)
normal DNA contentcrb2Δ26
normal double-strand break repair via nonhomologous end joiningcrb2Δ11
normal mitotic cell cycle regulation during cellular response to UVcrb2-1 (C1789T)13
normal mitotic cell cycle regulation during cellular response to UV during mitotic G1 phasecrb2Δ3
normal protein binding91
affecting rad4 and crb2V188P (V188P)
affecting rad3 and crb2crb2-BRCT (1-534)
normal protein kinase activity23
affecting activity of rad3crb2-BRCT(S666A) (aa 1-534 deleted, S666A)
normal protein localization to double-strand break site12
affecting rad9crb2Δ
affecting crb2crb2-T73A (T73A)
affecting crb2crb2-2AQ (T73A, S80A)
affecting crb2crb2-S80A (S80A)
affecting chk1crb2(1-358)-LZ (del aa 359-778 with C-terminal heterologous leucine zipper)
normal protein localization to nucleus during vegetative growth83
affecting hus1crb2Δ
normal protein phosphorylation during cellular response to bleomycin13
affecting ctp1crb2Δ
normal protein phosphorylation during cellular response to hydroxyurea15
affecting mre11crb2Δ
normal protein phosphorylation during cellular response to methyl methanesulfonate8
affecting mre11crb2Δ
normal protein phosphorylation during vegetative growth96
affecting hus1crb2Δ
normal punctate nuclear localization during cellular response to ionizing radiation2
affecting crb2crb2-2AQ (T73A, S80A)
affecting crb21-358 (359-778)
affecting crb2S234A (S234A)
normal telomere lengthcrb2Δ35
premature mitotic G1 phase entry during cellular response to ionizing radiationcrb2-2AQ (T73A, S80A)2
T215A (T215A)
T187A (T187A)
T235A (T235A)
shortened telomerescrb2Δ44
viable vegetative cell with normal cell morphologycrb2Δ3102

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased vegetative cell population growthcrb2Δ, csn1Δ
loss of viability at high temperaturecdc6-ts3 (G3191A), crb2Δ, set1::Kan
normal growth during cellular response to UVcrb2+, rad3+
normal growth on glucose carbon sourceS128A (S128A), crb2Δ, S129A (S129A)
crb2Δ, mdb1Δ
mdb1Δ, S129A (S129A), crb2Δ, S128A (S128A)
normal septation indexcnd2-1, crb2Δ
sensitive to camptothecinF400A (F400A), mdb1Δ
crb2Δ, csn1Δ
crb2-K619M (K619M), brc1Δ
crb2Δ, mdb1Δ
sensitive to hydroxyureaswd2Δ, crb2Δ
crb2Δ, spf1Δ
hta1-S129A (S129A), crb2Δ, hta2-S128A (S128A)
crb2Δ, set1::Kan
crb2Δ, csn1Δ
sensitive to ionizing radiation during vegetative growthrqh1Δ, crb2-T215A (T215A)
crb2-T215A (T215A), top3+
rqh1Δ, crb2Δ
crb2-T215A (T215A), cdc13-245 (E255R)
sensitive to methyl methanesulfonatecrb2Δ, csn1Δ
sensitive to UV during vegetative growthcrb2Δ, cut2-EA2 (D127A, 129E)
crb2Δ, csn1Δ
rqh1Δ, crb2Δ

Cell Phenotype

Term NameGenotypes
abnormal mitotic cell cycle regulation during cellular response to UVCrb2ΔC (547-778), cdc20-ts
swi7-ts, Crb2ΔC (547-778)
Crb2ΔC (547-778), cdc6-ts
abnormal mitotic sister chromatid segregationcnd2-1, crb2Δ
cutswi7-ts, Crb2ΔC (547-778)
Crb2ΔC (547-778), cdc20-ts
Crb2ΔC (547-778), cdc6-ts
decreased number of Rad51 foci during cellular response to ionizing radiationcrb2-T215A (T215A), rad50Δ
decreased protein localization to double-strand break site
affects localization of exo1crb2-F400A (F400A), wdr70Δ, spd1Δ
elongated telomerescrb2Δ, taz1Δ
inviable after spore germination, multiple cell divisionsrad52Δ, crb2Δ
normal DNA recombination frequency during cellular response to ionizing radiationrqh1Δ, crb2-T215A (T215A)
normal double-strand break repair via nonhomologous end joiningcrb2Δ, rad17Δ
normal mitotic cell cyclecnd2-1, crb2Δ
normal mitotic cell cycle regulation during cellular response to UVcrb2+, rad3+
normal mitotic G1/S phase transitioncnd2-1, crb2Δ
normal telomere lengthtel1Δ, crb2Δ
crb2Δ, mre11Δ
shortened telomerescrb2Δ, pku70Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in crb2 DNA repair protein Rad9 homolog Crb2
FYPO affected by mutation in dcr1 dicer
FYPO affected by mutation in hta1 histone H2A alpha
FYPO affected by mutation in hta2 histone H2A beta
FYPO affected by mutation in pdp1 PWWP domain protein Pdp1
FYPO affected by mutation in rad3 ATR checkpoint kinase Rad3
FYPO affected by mutation in rad4 BRCT domain protein Rad4
FYPO affected by mutation in set9 histone lysine H3-K20 methyltransferase Set9
FYPO localization affected by mutation in crb2 DNA repair protein Rad9 homolog Crb2
FYPO localization affected by mutation in pdp1 PWWP domain protein Pdp1
FYPO localization affected by mutation in rad4 BRCT domain protein Rad4
FYPO localization affected by mutation in spd1 ribonucleotide reductase (RNR) inhibitor
FYPO localization affected by mutation in wdr70 DDB1-CUL4-associated factor (DCAF), Wdr70
GO localized by set9 histone lysine H3-K20 methyltransferase Set9
GO substrate of cdc2 cyclin-dependent protein kinase Cdk1/Cdc2
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4210933..4213577
mRNA4210933..4213577
5' UTR4210933..4211086PMID:21511999
CDS4211087..4213423
3' UTR4213424..4213577PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00533 Pfam IPR001357 BRCT domain 536 640 5
SM00292 SMART IPR001357 BRCT domain 537 643 10
PS50172 Prosite Profiles IPR001357 BRCT domain 535 653 13
PS50172 Prosite Profiles IPR001357 BRCT domain 759 778 13
PTHR15321 HMMPANTHER 473 776 1
PTHR15321:SF3 HMMPANTHER 473 776 1
3.40.50.10190 Gene3D IPR001357 BRCT domain 536 654 13
SSF52113 SuperFamily IPR001357 BRCT domain 534 669 12

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000443BRCT domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000443
PBO:0001140tudor domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001140

Protein Properties

Ave. residue weight 112.42 Da
Charge 11.50
Codon Adaptation Index 0.39
Isoelectric point 8.26
Molecular weight 87.46 kDa
Number of residues 778
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS89
present during mitotic M phaseS43
present during mitotic M phaseS47
present during mitotic M phaseS88
present during mitotic M phaseS93
S80, S89, S93, S225, S253
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S89 PMID:21712547
present during mitotic M phase experimental evidence S43 PMID:21712547
present during mitotic M phase experimental evidence S47 PMID:21712547
present during mitotic M phase experimental evidence S88 PMID:21712547
present during mitotic M phase experimental evidence S93 PMID:21712547
IDA S225 PMID:25720772
IDA S80 PMID:22792081
IDA S253 PMID:25720772
experimental evidence S93 PMID:24763107
experimental evidence S89 PMID:24763107
O-phospho-L-threonineT73, T187, T215, T235 1085
Annotation ExtensionEvidenceResidueReference
IDA T73 PMID:22792081
IDA T215 PMID:24074952
IDA T187 PMID:24074952
IDA T235 PMID:24074952
O-phosphorylated residueS88,S93, S89,S93, S108,S111, T675,S677 2457
Annotation ExtensionEvidenceResidueReference
IDA S88,S93 PMID:25720772
IDA S108,S111 PMID:25720772
IDA T675,S677 PMID:25720772
IDA S89,S93 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for crb2 (SPBC342.05)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.65during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer148
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC342.05 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds to peptidechk1Chk1 protein kinase Protein-peptidePMID:22792081
affinity capturesrad4BRCT domain protein Rad4 Affinity Capture-WesternPMID:14739927
affinity capturescrb2DNA repair protein Rad9 homolog Crb2 Affinity Capture-WesternPMID:15229228
affinity captureschk1Chk1 protein kinase Affinity Capture-WesternPMID:22792081
affinity capturesrad9checkpoint clamp complex protein Rad9 Affinity Capture-WesternPMID:24663817
affinity captured byhta1histone H2A alpha Affinity Capture-WesternPMID:20679488
affinity captured byhta2histone H2A beta Affinity Capture-WesternPMID:20679488
affinity captured bychk1Chk1 protein kinase Affinity Capture-WesternPMID:14739927
affinity captured byrad3ATR checkpoint kinase Rad3 Affinity Capture-WesternPMID:14739927
forms complex withrad4BRCT domain protein Rad4 Reconstituted ComplexPMID:24663817
forms complex withhhf1histone H4 h4.1 Reconstituted ComplexPMID:18826944
forms complex withchk1Chk1 protein kinase Reconstituted ComplexPMID:22792081
forms complex withcrb2DNA repair protein Rad9 homolog Crb2 Reconstituted ComplexPMID:18676809
modified bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:24074952
modified byset9histone lysine H3-K20 methyltransferase Set9 Biochemical ActivityPMID:15550243
binds activation domain construct withptc1protein phosphatase 2C Ptc1 Two-hybridPMID:23695164
binds activation domain construct withcdc13G2/M B-type cyclin Cdc13 Two-hybridPMID:23695164
binds activation domain construct withcrb2DNA repair protein Rad9 homolog Crb2 Two-hybridPMID:15229228
binds DNA-binding domain construct withrad4BRCT domain protein Rad4 Two-hybridPMID:16778077
binds DNA-binding domain construct withchk1Chk1 protein kinase Two-hybridPMID:15229228
binds DNA-binding domain construct withsad1spindle pole body SUN domain protein Sad1 Two-hybridPMID:14655046
co-locates (via crystalisation or NMR) withrad4BRCT domain protein Rad4 Co-crystal or NMR structurePMID:24074952
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC342.05 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescueswdr70DDB1-CUL4-associated factor (DCAF), Wdr70 Phenotypic SuppressionPMID:27098497
rescueshhp1serine/threonine protein kinase Hhp1 Phenotypic SuppressionPMID:24861625
rescuescdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic SuppressionPMID:9407031
rescuescdc6DNA polymerase delta catalytic subunit Cdc6 Phenotypic SuppressionPMID:9407031
rescuespol1DNA polymerase alpha catalytic subunit Phenotypic SuppressionPMID:9407031
rescueschk1Chk1 protein kinase Phenotypic SuppressionPMID:12081644
synthetic growth defect withrqh1RecQ type DNA helicase Rqh1 Synthetic Growth DefectPMID:10373582
synthetic growth defect withbrc1BRCT domain protein Brc1 Synthetic Growth DefectPMID:26368543
synthetic growth defect withcsn1COP9/signalosome complex subunit Csn1 Synthetic Growth DefectPMID:25795664
synthetic growth defect withrad4BRCT domain protein Rad4 Synthetic Growth DefectPMID:24074952
synthetic growth defect withhta1histone H2A alpha Synthetic Growth DefectPMID:24806815
synthetic growth defect withexo1exonuclease I Exo1 Synthetic Growth DefectPMID:18931302
synthetic growth defect withtlg2SNARE Tlg2 (predicted) Synthetic Growth DefectPMID:18931302
synthetic growth defect withcdc2cyclin-dependent protein kinase Cdk1/Cdc2 Synthetic Growth DefectPMID:24861625
synthetic growth defect withhsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic Growth DefectPMID:20176980
synthetic growth defect withhta2histone H2A beta Synthetic Growth DefectPMID:20679485
synthetic growth defect withctp1CtIP-related endonuclease Synthetic Growth DefectPMID:21098122
synthetic growth defect withpof3F-box protein Pof3 Synthetic Growth DefectPMID:18931302
synthetic growth defect withcut2securin, sister chromatid separation inhibitor Synthetic Growth DefectPMID:15329725
synthetic growth defect withset9histone lysine H3-K20 methyltransferase Set9 Synthetic Growth DefectPMID:15550243
synthetic growth defect withrad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Synthetic Growth DefectPMID:12839619
synthetic growth defect withswi10DNA repair endonuclease non-catalytic subunit Swi10 Synthetic Growth DefectPMID:18931302
synthetic growth defect withwee1M phase inhibitor protein kinase Wee1 Synthetic Growth DefectPMID:20679488
synthetic growth defect withrhp41DNA repair protein Rhp41 Synthetic Growth DefectPMID:18931302
synthetic growth defect withswi3replication fork protection complex subunit Swi3 Synthetic Growth DefectPMID:18931302
synthetic growth defect withmus81Holliday junction resolvase subunit Mus81 Synthetic Growth DefectPMID:12589755
synthetic growth defect withcut1separase/separin Synthetic Growth DefectPMID:9153313
synthetic growth defect withrad57RecA family ATPase Rad57/Rhp57 Synthetic Growth DefectPMID:18931302
synthetic growth defect withrad51RecA family recombinase Rad51/Rhp51 Synthetic Growth DefectPMID:12839619
synthetic growth defect withswi1replication fork protection complex subunit Swi1 Synthetic Growth DefectPMID:14560029
synthetic growth defect withrad2FEN-1 endonuclease Rad2 Synthetic Growth DefectPMID:18931302
synthetic growth defect withmrc1mediator of replication checkpoint 1 Synthetic Growth DefectPMID:18931302
synthetic growth defect withmdb1BRCT domain protein Mdb1 Synthetic Growth DefectPMID:24806815
synthetic growth defect withrad13DNA repair nuclease Rad13 Synthetic Growth DefectPMID:9153313
synthetic growth defect withddb1damaged DNA binding protein Ddb1 Synthetic Growth DefectPMID:18931302
synthetic growth defect withrad3ATR checkpoint kinase Rad3 Synthetic Growth DefectPMID:21098122
synthetic growth defect withcut14condensin complex SMC subunit Smc2 Synthetic Growth DefectPMID:22645654
synthetic growth defect withrhp14XP-A family homolog Rhp14 Synthetic Growth DefectPMID:18931302
synthetic growth defect withsrs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:18931302
synthetic lethal withrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Synthetic LethalityPMID:10373582
synthetic lethal withhst4Sirtuin family histone deacetylase Hst4 Synthetic LethalityPMID:18344406
synthetic lethal withcdc27DNA polymerase delta subunit Cdc27 Synthetic LethalityPMID:9928931
synthetic lethal withcdc6DNA polymerase delta catalytic subunit Cdc6 Synthetic LethalityPMID:12589755
synthetic lethal withpol1DNA polymerase alpha catalytic subunit Synthetic LethalityPMID:9928931
synthetic lethal withpof3F-box protein Pof3 Synthetic LethalityPMID:11809834
rescued by overexpression ofchk1Chk1 protein kinase Dosage RescuePMID:9407031
rescued by overexpression oftop3DNA topoisomerase III Dosage RescuePMID:12023299
rescued by overexpression ofrad51RecA family recombinase Rad51/Rhp51 Dosage RescuePMID:12839619
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withhac12-hydroxyacyl-CoA lyase Hac1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
positive genetic interaction withppk31serine/threonine protein kinase Ppk31 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withpet801mitochondrial S-adenosylmethionine transmembrane transporter (predicted) Positive GeneticPMID:22681890
positive genetic interaction withayr11-acyldihydroxyacetone phosphate reductase (predicted) Positive GeneticPMID:22681890
positive genetic interaction witherd1Erd1 homolog (predicted) Positive GeneticPMID:22681890
positive genetic interaction withvsl1vacuolar SNARE Vsl1/Vam7 Positive GeneticPMID:22681890
positive genetic interaction withask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
positive genetic interaction withSPBC3H7.05cmitochondrial Membrane Bound O-Acyl Transferase (MBOAT) family Positive GeneticPMID:22681890
positive genetic interaction withsum2translation initiation inhibitor (predicted) Positive GeneticPMID:22681890
positive genetic interaction withstm1G-protein coupled receptor Stm1 Positive GeneticPMID:22681890
positive genetic interaction withSPAC227.17cconserved eukaryotic protein, DUF3128, human c22orf39 ortholog Positive GeneticPMID:22681890
positive genetic interaction withSPAC1F12.04cconserved fungal protein Positive GeneticPMID:22681890
positive genetic interaction withprz1calcineurin responsive transcription factor Prz1 Positive GeneticPMID:22681890
positive genetic interaction withslx1structure-specific endonuclease catalytic subunit Slx1 Positive GeneticPMID:22681890
positive genetic interaction withsdu1PPPDE peptidase family (predicted) Positive GeneticPMID:22681890
positive genetic interaction withhip4histone promoter control protein Hip4 Positive GeneticPMID:22681890
positive genetic interaction withspo15mitotic and mieotic spindle pole body protein Spo15 Positive GeneticPMID:22681890
positive genetic interaction withnas226S proteasome regulatory particle assembly protein Nas2 (predicted) Positive GeneticPMID:22681890
synthetically rescued byhta2histone H2A beta Synthetic RescuePMID:24806815
synthetically rescued bybdf2BET family double bromodomain protein Bdf2 Synthetic RescuePMID:22095079
synthetically rescued byhta1histone H2A alpha Synthetic RescuePMID:24806815
synthetically rescued byspp1DNA primase catalytic subunit Spp1 Synthetic RescuePMID:12589755
synthetically rescued bybdf1Swr1 complex bromodomain subunit Bdf1 Synthetic RescuePMID:22095079
synthetically rescued bychk1Chk1 protein kinase Synthetic RescuePMID:15229228
synthetically rescued byrqh1RecQ type DNA helicase Rqh1 Synthetic RescuePMID:12023299
synthetically rescued bymdb1BRCT domain protein Mdb1 Synthetic RescuePMID:24806815
synthetically rescued byset1histone lysine methyltransferase Set1 Synthetic RescuePMID:12589755
synthetically rescued byswd2Set1C complex subunit Swd2.1 Synthetic RescuePMID:12589755
negative genetic interaction withfml1ATP-dependent 3' to 5' DNA helicase, FANCM ortholog Fml1 Negative GeneticPMID:22681890
negative genetic interaction withcsn2COP9/signalosome complex subunit Csn2 Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
negative genetic interaction withfml2ATP-dependent 3' to 5' DNA helicase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withexo1exonuclease I Exo1 Negative GeneticPMID:22681890
negative genetic interaction withmcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:18818364
negative genetic interaction withlsg1Lsk1 complex gamma subunit Lsg1 Negative GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction withrad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
negative genetic interaction withrsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
negative genetic interaction withngg1SAGA complex subunit Ngg1/Ada3 Negative GeneticPMID:22681890
negative genetic interaction withrad51RecA family recombinase Rad51/Rhp51 Negative GeneticPMID:18818364
negative genetic interaction withrhp7Rad7 homolog Rhp7 Negative GeneticPMID:18818364
negative genetic interaction withrad57RecA family ATPase Rad57/Rhp57 Negative GeneticPMID:22681890
negative genetic interaction withSPAC750.08cNAD-dependent malic enzyme (predicted), partial Negative GeneticPMID:22681890
negative genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
negative genetic interaction withgrx3monothiol glutaredoxin Grx3 Negative GeneticPMID:22681890
negative genetic interaction withsrs2ATP-dependent DNA helicase, UvrD subfamily Negative GeneticPMID:18818364
negative genetic interaction withrhp14XP-A family homolog Rhp14 Negative GeneticPMID:18818364
negative genetic interaction withdcd1deoxycytidylate deaminase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withapc10anaphase-promoting complex substrate recognition subunit Apc10 Negative GeneticPMID:22681890
negative genetic interaction withmre11Mre11 nuclease Negative GeneticPMID:18818364
negative genetic interaction withSPBC16H5.12cconserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withptp4phosphatidate cytidylyltransferase Ptp4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrhp23Rad23 homolog Rhp23 Negative GeneticPMID:22681890
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
negative genetic interaction withrem1meiosis-specific cyclin Rem1 Negative GeneticPMID:22681890
negative genetic interaction withemc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC212.04cS. pombe specific DUF999 family protein 1 Negative GeneticPMID:22681890
negative genetic interaction withhrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrps40140S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmrc1mediator of replication checkpoint 1 Negative GeneticPMID:18818364
negative genetic interaction withyps1aspartic protease, yapsin Yps1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC1348.03S. pombe specific 5Tm protein family Negative GeneticPMID:22681890
negative genetic interaction withpdc102pyruvate decarboxylase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps38phophatidylinositol 3-kinase complex subunit Vps38 Negative GeneticPMID:22681890
negative genetic interaction withSPCC1020.05phosphoprotein phosphatase involved in unfolded protein response (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withrod1arrestin/PY protein involved in ubiquitin-mediated endocytosis Rod1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withswi3replication fork protection complex subunit Swi3 Negative GeneticPMID:18818364
overexpression rescuesrad1checkpoint clamp complex protein Rad1 Dosage RescuePMID:12839619
overexpression rescuesrad4BRCT domain protein Rad4 Dosage RescuePMID:9407031
overexpression rescuesrad9checkpoint clamp complex protein Rad9 Dosage RescuePMID:12839619
overexpression rescuesrad17RFC related checkpoint protein Rad17 Dosage RescuePMID:12839619
overexpression rescueshus1checkpoint clamp complex protein Hus1 Dosage RescuePMID:12839619
phenotype enhanced bypcn1PCNA Phenotypic EnhancementPMID:9928931
phenotype enhanced byhta1histone H2A alpha Phenotypic EnhancementPMID:16314498
phenotype enhanced bypol1DNA polymerase alpha catalytic subunit Phenotypic EnhancementPMID:9928931
phenotype enhanced bycdc24DNA replication protein Cdc24 Phenotypic EnhancementPMID:9928931
phenotype enhanced bycdc18MCM loader Phenotypic EnhancementPMID:9928931
phenotype enhanced bycdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:9928931
phenotype enhanced bycdc10MBF transcription factor complex subunit Cdc10 Phenotypic EnhancementPMID:9928931
phenotype enhanced bycdc17ATP-dependent DNA replication ligase Cdc17 Phenotypic EnhancementPMID:9928931
phenotype enhanced byhta2histone H2A beta Phenotypic EnhancementPMID:16314498
phenotype enhanced bycdc27DNA polymerase delta subunit Cdc27 Phenotypic EnhancementPMID:9928931
synthetically rescueschk1Chk1 protein kinase Synthetic RescuePMID:16816416
enhances phenotype oforc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
enhances phenotype ofcdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:14739927
enhances phenotype ofctp1CtIP-related endonuclease Phenotypic EnhancementPMID:21098122
enhances phenotype ofcdc6DNA polymerase delta catalytic subunit Cdc6 Phenotypic EnhancementPMID:14739927
enhances phenotype ofpol1DNA polymerase alpha catalytic subunit Phenotypic EnhancementPMID:14739927
enhances phenotype ofrad3ATR checkpoint kinase Rad3 Phenotypic EnhancementPMID:21098122
rescued byhta2histone H2A beta Phenotypic SuppressionPMID:16314498
rescued bypku70Ku domain protein Pku70 Phenotypic SuppressionPMID:12196391
rescued byhta1histone H2A alpha Phenotypic SuppressionPMID:16314498
rescued bytaz1human TRF ortholog Taz1 Phenotypic SuppressionPMID:12196391
External References
Database Identifier Description
NBRP SPBC342.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC342.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC342.05 BioGRID Interaction Datasets
Expression Viewer SPBC342.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC342.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC342.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC342.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC342.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC342.05 Transcriptome Viewer (Bähler Lab)
GEO SPBC342.05 GEO profiles
PInt SPBC342.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC342.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC342.05 Fission yeast phenotypic data & analysis
Cyclebase SPBC342.05.1 Cell Cycle Data
SPD / RIKEN33/33G08Orfeome Localization Data
UniProtKB/SwissProtP87074DNA repair protein crb2
ModBaseP87074Database of comparative protein structure models
STRINGP87074Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596748DNA repair protein
RefSeq mRNANM_001023768972h- DNA repair protein (crb2), mRNA
ePDB2FHDThe European PDB
PDB2FHDPDB
PDBsum2FHDPDBsum
ePDB2VXBThe European PDB
PDB2VXBPDB
PDBsum2VXBPDBsum
ePDB2VXCThe European PDB
PDB2VXCPDB
PDBsum2VXCPDBsum
ePDB4BU0The European PDB
PDB4BU0PDB
PDBsum4BU0PDBsum
ePDB4BU1The European PDB
PDB4BU1PDB
PDBsum4BU1PDBsum
European Nucleotide ArchiveBAA13093.1ENA Protein Mapping
European Nucleotide ArchiveCAA70582.1ENA Protein Mapping
European Nucleotide ArchiveCAB46775.1ENA Protein Mapping
UniParcUPI00001338AAUniProt Archive

Literature for crb2

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016