crb2 (SPBC342.05)

Gene Standard Namecrb2 ChromosomeII
Systematic IDSPBC342.05 Gene Start4210933
Synonymsrhp9 Gene End4213577
ProductDNA repair protein Rad9 homolog, Rhp9 Gene Length2645
Feature Typeprotein coding CDS Start4211087
Name Description CDS End4213423
Characterisation Statuspublished CDS Length2337

Ensembl Gene Location
  1. SPNCRNA.576
  2. qrs1
  3. gas4
  4. rpn1301
  5. SPBTRNAASN.04
  6. SPRRNA.38
  7. crb2
  8. rtt109
  9. SPNCRNA.1680
  10. rpn12
  11. SPBC16G5.02c
  12. SPBC16G5.03
  13. mrpl23
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000266sensitive to DNA damaging agentscompetitive growth assay evidencecrb2delta (deletion)deletionPMID:20537132
FYPO:0000268sensitive to UVcompetitive growth assay evidencecrb2delta (deletion)deletionPMID:20537132
FYPO:0001491viable vegetative cellno_name (unrecorded)unrecordedPMID:9153313
Microscopycrb2delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0005515protein bindingIPIrad3PMID:14739927
IPIchk1PMID:9407031
IPIrad4PMID:9407031
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000723telomere maintenanceIMPPMID:10373582
GO:0007095mitotic G2 DNA damage checkpointIMPPMID:9153313
GO:0008156negative regulation of DNA replicationIEAUniProtKB:KW-0236GO_REF:0000037
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0000790nuclear chromatinISMPFAM:PF00533GO_REF:0000001
GO:0005634nucleusIDAPMID:16823372
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
142109334213577

References

Region Start End Reference
three_prime_UTR42134244213577PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00533 IPR001357 Pfam BRCT domain 536 640 8
SM00292 IPR001357 SMART BRCT domain 537 643 12
PS50172 IPR001357 Prosite Profiles BRCT domain 535 653 13
PS50172 IPR001357 Prosite Profiles BRCT domain 759 778 13
G3DSA:3.40.50.10190 Gene3D 536 654 12
SSF52113 IPR001357 SuperFamily 534 669 12
PTHR15321 hmmpanther 1 777 1
PTHR15321:SF0 hmmpanther 1 777 1

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residue

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0000443BRCT domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000443
PBO:0001140tudor domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001140

Protein Properties

Ave. residue weight 112.42 Da
Charge 11.50
Isoelectric point 8.26
Molecular weight 87.46 kDa
Number of residues 778
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
experimental evidencevegetative growth of a single-celled organismPECO:0000005,
PECO:0000014		population_widePMID:23101633
RNA levelexperimental evidencevegetative growth of a single-celled organismPECO:0000014,
PECO:0000005		population_wide1.6PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.65PMID:23101633
Disease Association
DescriptionQualifierReference
cancer
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
srs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
rad13DNA repair nuclease Rad13 Synthetic Growth DefectPMID:9153313
pol1DNA polymerase alpha catalytic subunit Phenotypic EnhancementPMID:14739927
Synthetic LethalityPMID:9928931
Phenotypic Enhancement
Phenotypic SuppressionPMID:9407031
prz1calcineurin responsive transcription factor Prz1 Positive GeneticPMID:22681890
rad2FEN-1 endonuclease Rad2 Synthetic Growth DefectPMID:9153313
Synthetic Growth DefectPMID:18931302
rad17RFC related checkpoint protein Rad17 Dosage RescuePMID:12839619
cdc24DNA replication protein Cdc24 Phenotypic EnhancementPMID:9928931
set1histone lysine methyltransferase Set1 Synthetic RescuePMID:12589755
SPBC725.04oxalyl-CoA decarboxylase (predicted) Positive GeneticPMID:22681890
mus81Holliday junction resolvase subunit Mus81 Synthetic Growth DefectPMID:12589755
hst4Sir2 family histone deacetylase Hst4 Synthetic LethalityPMID:18344406
mcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:18818364
cdc27DNA polymerase delta subunit Cdc27 Phenotypic EnhancementPMID:9928931
Synthetic Lethality
cdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:18667534
Phenotypic EnhancementPMID:14739927
Phenotypic EnhancementPMID:9928931
Phenotypic SuppressionPMID:9407031
ayr11-acyldihydroxyacetone phosphate reductase (predicted) Positive GeneticPMID:22681890
lsg1Lsk1 complex gamma subunit (predicted) Negative GeneticPMID:22681890
hta1histone H2A alpha Synthetic Growth DefectPMID:20679485
Synthetic Growth DefectPMID:16778077
Synthetic Rescue
Phenotypic EnhancementPMID:16314498
Phenotypic Suppression
ctp1CtIP-related endonuclease Phenotypic EnhancementPMID:21098122
Synthetic Growth Defect
SPAC186.09pyruvate decarboxylase (predicted) Negative GeneticPMID:22681890
wee1M phase inhibitor protein kinase Wee1 Synthetic Growth DefectPMID:20679488
erd1Erd1 homolog (predicted) Positive GeneticPMID:22681890
pof3F-box protein Pof3 Synthetic Growth DefectPMID:18931302
Synthetic LethalityPMID:11809834
rhp14XP-A family homolog Rhp14 Negative GeneticPMID:22681890
Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
fml2ATP-dependent 3' to 5' DNA helicase (predicted) Negative GeneticPMID:22681890
bdf1Swr1 complex bromodomain subunit Brf1 Synthetic RescuePMID:22095079
rad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
atg14autophagy specific phophatidylinositol 3-kinase complex subunit Atg14 (predicted) Negative GeneticPMID:22681890
SPAC1F12.04cconserved fungal protein Positive GeneticPMID:22681890
mrc1mediator of replication checkpoint 1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
rad4BRCT domain protein Rad4 Dosage RescuePMID:9407031
Synthetic Growth DefectPMID:16778077
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
ppk31serine/threonine protein kinase Ppk31 (predicted) Positive GeneticPMID:18818364
rhp7Rad7 homolog Rhp7 Negative GeneticPMID:18818364
SPAC12B10.09S-adenosylmethionine transporter (predicted) Positive GeneticPMID:22681890
pcn1PCNA Phenotypic EnhancementPMID:9928931
grx3monothiol glutaredoxin Grx3 Negative GeneticPMID:22681890
dcd1deoxycytidylate deaminase (predicted) Negative GeneticPMID:22681890
cdc10MBF transcription factor complex subunit Cdc10 Phenotypic EnhancementPMID:9928931
swd2Set1C complex subunit Swd2.1 Synthetic RescuePMID:12589755
csn2COP9/signalosome complex subunit Csn2 Negative GeneticPMID:22681890
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Synthetic Growth DefectPMID:12839619
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
cdc17ATP-dependent DNA ligase Cdc17 Phenotypic EnhancementPMID:9928931
tlg2SNARE Tlg2 (predicted) Synthetic Growth DefectPMID:18931302
rad57RecA family ATPase Rad57/Rhp57 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
SPAC31A2.12arrestin/PY protein 1 (predicted) Negative GeneticPMID:22681890
hrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
rsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
SPCC594.06cvacuolar SNARE Vam7 (predicted) Positive GeneticPMID:22681890
swi3replication fork protection complex subunit Swi3 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
cut1separase/separin Synthetic Growth DefectPMID:9153313
slx1structure-specific endonuclease catalytic subunit Positive GeneticPMID:22681890
SPBC1348.03S. pombe specific 5Tm protein family Negative GeneticPMID:22681890
SPBC3H7.05cmitochondrial Membrane Bound O-Acyl Transferase (MBOAT) family Positive GeneticPMID:22681890
orc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
rem1meiosis-specific cyclin Rem1 Negative GeneticPMID:22681890
swi10DNA repair endonuclease Swi10 Synthetic Growth DefectPMID:18931302
ams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Dosage RescuePMID:12839619
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
yps1aspartic protease, yapsin Yps1 Negative GeneticPMID:22681890
SPAC2H10.02c26S proteasome regulatory particle assembly protein (predicted) Positive GeneticPMID:22681890
fml1ATP-dependent 3' to 5' DNA helicase, FANCM ortholog Fml1 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
SPAC227.17cconserved protein Positive GeneticPMID:22681890
set9histone lysine methyltransferase Set9 Synthetic Growth DefectPMID:15550243
Synthetic Growth DefectPMID:16778077
stm1G-protein coupled receptor Stm1 Positive GeneticPMID:22681890
apc10anaphase-promoting complex subunit Apc10 Negative GeneticPMID:22681890
cut14condensin complex subunit Cut14 Synthetic Growth DefectPMID:22645654
cut2securin, sister chromatid separation inhibitor Synthetic Growth DefectPMID:15329725
chk1Chk1 protein kinase Synthetic RescuePMID:15229228
Dosage RescuePMID:9407031
Synthetic RescuePMID:16816416
Phenotypic SuppressionPMID:12081644
top3DNA topoisomerase III Dosage RescuePMID:12023299
SPBC16H5.12cconserved fungal protein Negative GeneticPMID:22681890
mre11Mre11 nuclease Negative GeneticPMID:18818364
hta2histone H2A beta Synthetic Growth DefectPMID:20679485
Synthetic RescuePMID:16778077
Synthetic Growth Defect
Phenotypic EnhancementPMID:16314498
Phenotypic Suppression
sum2G2/M transition checkpoint protein Sum2 Positive GeneticPMID:22681890
ptp4phosphatidate cytidylyltransferase Ptp4 (predicted) Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
mug67PPPDE peptidase family (predicted) Positive GeneticPMID:22681890
nrc1bromodomain protein (predicted) Synthetic RescuePMID:22095079
rad1checkpoint clamp complex protein Rad1 Dosage RescuePMID:12839619
rhp23Rad23 homolog Rhp23 Negative GeneticPMID:22681890
SPAC212.04cS. pombe specific DUF999 family protein 1 Negative GeneticPMID:22681890
rad9checkpoint clamp complex protein Rad9 Dosage RescuePMID:12839619
rad3ATR checkpoint kinase Rad3 Synthetic Growth DefectPMID:21098122
Phenotypic Enhancement
rhp41DNA repair protein Rhp41 Synthetic Growth DefectPMID:18931302
spp1DNA primase catalytic subunit Spp1 Synthetic RescuePMID:12589755
SPCC1020.05phosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
rad51RecA family recombinase Rad51/Rhp51 Synthetic Growth DefectPMID:12839619
Dosage Rescue
Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
rps40140S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
swi1replication fork protection complex subunit Swi1 Synthetic Growth DefectPMID:14560029
ddb1damaged DNA binding protein Ddb1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
taz1human TRF ortholog Taz1 Phenotypic SuppressionPMID:12196391
spo15sporulation protein Spo15 Positive GeneticPMID:22681890
pku70Ku domain protein Pku70 Phenotypic SuppressionPMID:12196391
emc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic Growth DefectPMID:20176980
rqh1RecQ type DNA helicase Rqh1 Synthetic RescuePMID:12023299
Synthetic Growth DefectPMID:12589755
cdc6DNA polymerase delta catalytic subunit Cdc6 Phenotypic EnhancementPMID:14739927
Phenotypic SuppressionPMID:9407031
Synthetic LethalityPMID:12589755
SPAC750.08cNAD-dependent malic enzyme (predicted), partial Negative GeneticPMID:22681890
exo1exonuclease I Exo1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
cdc18MCM loader Phenotypic EnhancementPMID:9928931
hip4histone promoter control protein Hip4 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rad4BRCT domain protein Rad4 Two-hybridPMID:15229228
Reconstituted ComplexPMID:9407031
Two-hybrid
Co-localizationPMID:16778077
Two-hybrid
set9histone lysine methyltransferase Set9 Biochemical ActivityPMID:15550243
rad22DNA recombination protein Rad22 (previously Rad52) Co-localizationPMID:12917337
hta1histone H2A alpha Affinity Capture-WesternPMID:20679488
chk1Chk1 protein kinase Affinity Capture-WesternPMID:14739927
Reconstituted ComplexPMID:22792081
Affinity Capture-Western
Two-hybridPMID:15229228
Two-hybridPMID:9407031
Affinity Capture-WesternPMID:23422000
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:10488332
rad3ATR checkpoint kinase Rad3 Affinity Capture-WesternPMID:14739927
sad1spindle pole body protein Sad1 Two-hybridPMID:14655046
hhf2histone H4 h4.2 Co-localizationPMID:16778077
hta2histone H2A beta Affinity Capture-WesternPMID:20679488
hhf1histone H4 h4.1 Reconstituted ComplexPMID:18826944
Co-localizationPMID:16778077
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External References
Database Identifier Description
NBRP SPBC342.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC342.05 Retrieval of eukaryotic orthologs
BioGrid SPBC342.05 BioGRID Interaction Datasets
Bähler Lab SPBC342.05 Cell Cycle Expression Profile
Bähler Lab SPBC342.05 Meiosis/Sporulation Expression Profies
Bähler Lab SPBC342.05 Pheromone response/mating expression profiles
Bähler Lab SPBC342.05 Environmental stress expression profiles
Bähler Lab SPBC342.05 Bähler Lab Transcriptome Viewer
Cyclebase SPBC342.05 Cell Cycle Data
PInt SPBC342.05 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPBC342.05 Entrez Gene
SPD / RIKEN33/33G08Orfeome Localization Data
EntrezGene2540992DNA repair protein
Uniprot_genenameRHP9
WikiGene2540992DNA repair protein
UniProtKB/SwissProtP87074DNA repair protein rhp9
ModBaseP87074Database of comparative protein structure models
Pfam Protein DomainsP87074Pfam Domain Arrangement
RefSeq PeptideNP_596748DNA repair protein
RefSeq mRNANM_001023768972h- DNA repair protein (crb2), mRNA
European Nucleotide ArchiveBAA13093ENA Protein Mapping
European Nucleotide ArchiveCAA70582ENA Protein Mapping
European Nucleotide ArchiveCAB46775ENA Protein Mapping
SPD / RIKEN33/33G08Orfeome Localization Data

Literature for crb2
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Search: UK PMC or PubMed

Release Version: PomBase:18.34 - 04 Apr 2013