spo9 (SPBC36.06c)


Gene Standard Namespo9 Characterisation Statuspublished
Systematic IDSPBC36.06c Feature Typeprotein coding
Synonyms Name Description
Productfarnesyl pyrophosphate synthetase Product Size351aa, 40.95 kDa
Genomic Location Chromosome II, 853592-851808 (1785nt); CDS:853237-852025 (1213nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
dimethylallyltranstransferase activity2
Annotation ExtensionEvidenceWith/FromReference
farnesyltranstransferase activity3
Annotation ExtensionEvidenceWith/FromReference
geranyltranstransferase activity2
Annotation ExtensionEvidenceWith/FromReference
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
protein farnesyltransferase activity4
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ascospore formation75
Annotation ExtensionEvidenceWith/FromReference
carotenoid biosynthetic process1
Annotation ExtensionEvidenceWith/FromReference
farnesyl diphosphate biosynthetic process4
Annotation ExtensionEvidenceWith/FromReference
geranyl diphosphate biosynthetic process2
Annotation ExtensionEvidenceWith/FromReference
geranylgeranyl diphosphate biosynthetic process1
Annotation ExtensionEvidenceWith/FromReference
protein prenylation7
Annotation ExtensionEvidenceWith/FromReference
protein targeting to plasma membrane7
Annotation ExtensionEvidenceWith/FromReference
steroid metabolic process47
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000082decreased cell population growth at high temperatureCell growth assayspo9ΔNullPECO:0000201, PECO:0000102PMID:23145048148
FYPO:0001164normal growth on glucose carbon sourceCell growth assayspo9ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:23145048176
FYPO:0002640sensitive to clotrimazole105
expressivity FYPO_EXT:0000002Cell growth assayspo9ΔNullPECO:0000137, PECO:0000005PMID:22252817
FYPO:0002641sensitive to micafunginCell growth assayspo9ΔNullPECO:0000137, PECO:0000005PMID:22252817116
FYPO:0000110sensitive to pravastatinCell growth assayspo9ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:231450482
FYPO:0002328sensitive to terbinafine101
expressivity FYPO_EXT:0000001Cell growth assayspo9ΔNullPECO:0000137, PECO:0000005PMID:22252817
Cell growth assayspo9ΔNullPECO:0000005, PECO:0000201PMID:23145048
FYPO:0000091sensitive to thiabendazolecompetitive growth assay evidencespo9ΔNullPMID:20537132176
FYPO:0002060viable vegetative cell populationMicroscopyspo9ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopyspo9ΔNullPMID:20473289
Microscopynot recorded (unrecorded)PMID:17596513

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002314GGPP absent from cellSubstance quantification evidencespo9ΔNullPMID:231450481
FYPO:0002318increased cellular ergosterol levelSubstance quantification evidencespo9ΔNullPMID:231450484
FYPO:0002317increased cellular squalene levelSubstance quantification evidencespo9ΔNullPMID:231450481
FYPO:0001801inviable cell at high temperatureCell growth assayspo9ΔNullPECO:0000201, PECO:0000102PMID:2314504823
FYPO:0002315normal cellular FPP levelSubstance quantification evidencespo9ΔNullPMID:231450481
FYPO:0001491viable vegetative cellCell growth assayspo9ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:231450483607
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyspo9ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1853592853103
2853053852916
3852847852647
4852606851808

UTRs

Region Coordinates Reference
five_prime_UTR853592..853238PMID:21511999
three_prime_UTR852024..851808PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00348 Pfam IPR000092 Polyprenyl synthetase 49 311 3
PS00444 Prosite Patterns IPR000092 Polyprenyl synthetase 231 243 3
PS00723 Prosite Patterns IPR000092 Polyprenyl synthetase 97 111 3
PTHR11525 HMMPANTHER 12 351 2
PTHR11525:SF0 HMMPANTHER 12 351 2
1.10.600.10 Gene3D IPR008949 Terpenoid synthase 13 350 5
SSF48576 SuperFamily IPR008949 Terpenoid synthase 13 350 5

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.66 Da
Charge -5.50
Isoelectric point 5.07
Molecular weight 40.95 kDa
Number of residues 351
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1118during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
956during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
475.63during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
783during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.25during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
fps1geranyltranstransferase Fps1 Affinity Capture-WesternPMID:17596513
Two-hybrid
spo9farnesyl pyrophosphate synthetase Two-hybridPMID:17596513
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
xap5xap-5-like protein Negative GeneticPMID:24957674
fps1geranyltranstransferase Fps1 Dosage RescuePMID:17596513
tsc2tuberin Dosage RescuePMID:16624901
cpp1protein farnesyltransferase beta subunit Cpp1 Dosage RescuePMID:16624901
External References
Database Identifier Description
NBRP SPBC36.06c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC36.06c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC36.06c BioGRID Interaction Datasets
Expression Viewer SPBC36.06c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC36.06c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC36.06c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC36.06c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC36.06c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC36.06c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC36.06c Cell Cycle Data
GEO SPBC36.06c GEO profiles
PInt SPBC36.06c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC36.06c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC36.06c Fission yeast phenotypic data & analysis
IntEnz2.5.1.1Integrated relational Enzyme database
Rhea2.5.1.1Annotated reactions database
SPD / RIKEN22/22B05Orfeome Localization Data
UniProtKB/SwissProtO59703Geranylgeranyl pyrophosphate synthase
ModBaseO59703Database of comparative protein structure models
STRINGO59703Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595334farnesyl pyrophosphate synthetase
RefSeq mRNANM_001021242972h- farnesyl pyrophosphate synthetase (spo9), mRNA
European Nucleotide ArchiveCAA19054.1ENA Protein Mapping
UniParcUPI000006AA57UniProt Archive

Literature for spo9

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014