spo9 (SPBC36.06c)


Gene Standard Namespo9 Characterisation Statuspublished
Systematic IDSPBC36.06c Feature Typeprotein coding
Synonyms Name Description
Productfarnesyl pyrophosphate synthetase Product Size351aa, 40.95 kDa
Genomic Location Chromosome II, 853592-851808 (1785nt); CDS:853237-852025 (1213nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004161dimethylallyltranstransferase activityIEA EC:2.5.1.1GO_REF:00000032
GO:0004311farnesyltranstransferase activityIEA EC:2.5.1.29GO_REF:00000033
GO:0004337geranyltranstransferase activityIEA EC:2.5.1.10GO_REF:00000032
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO:0005515protein bindingIPIfps1PMID:17596513865
GO:0004660protein farnesyltransferase activityIMPPMID:175965134
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030437ascospore formationIMPPMID:1759651367
GO:0016117carotenoid biosynthetic processIEAUniProtKB-KW:KW-0125GO_REF:00000371
GO:0045337farnesyl diphosphate biosynthetic processIEA UniPathway:UPA00260GO_REF:00000414
GO:0033384geranyl diphosphate biosynthetic processIEA UniPathway:UPA00259GO_REF:00000412
GO:0033386geranylgeranyl diphosphate biosynthetic processIMPPMID:231450481
GO:0018342protein prenylationIMPPMID:175965137
GO:0072661protein targeting to plasma membraneICGO:0018342GO_REF:00000017
GO:0008202steroid metabolic processNASGO_REF:000005142
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722319
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000082decreased cell population growth at high temperatureCell growth assayspo9ΔNullPECO:0000201, PECO:0000102PMID:23145048144
FYPO:0001164normal growth on glucose carbon sourceCell growth assayspo9ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:23145048165
FYPO:0002640sensitive to clotrimazole105
expressivity FYPO_EXT:0000002Cell growth assayspo9ΔNullPECO:0000137, PECO:0000005PMID:22252817
FYPO:0002641sensitive to micafunginCell growth assayspo9ΔNullPECO:0000137, PECO:0000005PMID:22252817116
FYPO:0000110sensitive to pravastatinCell growth assayspo9ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:231450482
FYPO:0002328sensitive to terbinafine101
expressivity FYPO_EXT:0000001Cell growth assayspo9ΔNullPECO:0000137, PECO:0000005PMID:22252817
Cell growth assayspo9ΔNullPECO:0000005, PECO:0000201PMID:23145048
FYPO:0000091sensitive to thiabendazolecompetitive growth assay evidencespo9ΔNullPMID:20537132175
FYPO:0002060viable vegetative cell populationMicroscopyspo9ΔNullPECO:0000005, PECO:0000137PMID:236978063751
Microscopyspo9ΔNullPMID:20473289
Microscopynot recorded (unrecorded)PMID:17596513

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002314GGPP absent from cellSubstance quantification evidencespo9ΔNullPMID:231450481
FYPO:0002318increased cellular ergosterol levelSubstance quantification evidencespo9ΔNullPMID:231450484
FYPO:0002317increased cellular squalene levelSubstance quantification evidencespo9ΔNullPMID:231450481
FYPO:0001801inviable cell at high temperatureCell growth assayspo9ΔNullPECO:0000201, PECO:0000102PMID:2314504823
FYPO:0002315normal cellular FPP levelSubstance quantification evidencespo9ΔNullPMID:231450481
FYPO:0001491viable vegetative cellCell growth assayspo9ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:231450483599
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyspo9ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1853592853103
2853053852916
3852847852647
4852606851808
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00348 Pfam IPR000092 Polyprenyl synthetase 49 311 3
PS00723 Prosite Patterns IPR000092 Polyprenyl synthetase 97 111 3
PS00444 Prosite Patterns IPR000092 Polyprenyl synthetase 231 243 3
PTHR11525:SF0 HMMPANTHER 12 351 2
PTHR11525 HMMPANTHER 12 351 2
1.10.600.10 Gene3D IPR008949 Terpenoid synthase 13 350 5
SSF48576 SuperFamily IPR008949 Terpenoid synthase 13 350 5

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.66 Da
Charge -5.50
Isoelectric point 5.07
Molecular weight 40.95 kDa
Number of residues 351
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1118during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
956during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
783during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
475.63during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.25during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
xap5xap-5-like protein Negative GeneticPMID:24957674
fps1geranyltranstransferase Fps1 Dosage RescuePMID:17596513
tsc2tuberin Dosage RescuePMID:16624901
cpp1protein farnesyltransferase beta subunit Cpp1 Dosage RescuePMID:16624901
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
fps1geranyltranstransferase Fps1 Two-hybridPMID:17596513
Affinity Capture-Western
spo9farnesyl pyrophosphate synthetase Two-hybridPMID:17596513
External References
Database Identifier Description
NBRP SPBC36.06c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC36.06c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC36.06c BioGRID Interaction Datasets
Expression Viewer SPBC36.06c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC36.06c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC36.06c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC36.06c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC36.06c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC36.06c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC36.06c Cell Cycle Data
GEO SPBC36.06c GEO profiles
PInt SPBC36.06c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC36.06c Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz2.5.1.1Integrated relational Enzyme database
Rhea2.5.1.1Annotated reactions database
SPD / RIKEN22/22B05Orfeome Localization Data
UniProtKB/SwissProtO59703Geranylgeranyl pyrophosphate synthase
ModBaseO59703Database of comparative protein structure models
STRINGO59703Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595334farnesyl pyrophosphate synthetase
RefSeq mRNANM_001021242972h- farnesyl pyrophosphate synthetase (spo9), mRNA
European Nucleotide ArchiveCAA19054.1ENA Protein Mapping
UniParcUPI000006AA57UniProt Archive

Literature for spo9

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014