spo9 (SPBC36.06c)

Gene Standard Namespo9 Characterisation Statuspublished
Systematic IDSPBC36.06c Feature Typeprotein coding
Synonyms Name Description
Productfarnesyl pyrophosphate synthetase Product Size351aa, 40.95 kDa
Genomic Location Chromosome II, 853592-851808 (1785nt); CDS:853237-852025 (1213nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
farnesyltranstransferase activity4
Annotation ExtensionEvidenceWith/FromReference
geranyltranstransferase activity2
Annotation ExtensionEvidenceWith/FromReference
metal ion binding751
Annotation ExtensionEvidenceWith/FromReference
protein binding868
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at high temperaturespo9Δ172
inviable vegetative cell populationspo9Δ1451
normal growth on glucose carbon sourcespo9Δ282
sensitive to clotrimazolespo9Δ107
sensitive to micafunginspo9Δ116
sensitive to pravastatinspo9Δ2
sensitive to terbinafinespo9Δ103
sensitive to thiabendazolespo9Δ326
slow vegetative cell population growthspo9Δ357
viable vegetative cell populationspo9-B2613840

Cell Phenotype

Term NameGenotypesCount
abnormal prospore membrane formationspo9-B26130
abnormal sporulationspo9Δ173
decreased farnesyltranstransferase activityspo9Δ1
spo9-R109Q (R109Q)
decreased protein localization to plasma membrane, with protein mislocalized to cytoplasm, during vegetative growth1
affects localization of ypt7spo9Δ
GGPP absent from cellspo9Δ1
increased cellular ergosterol levelspo9Δ4
increased cellular squalene levelspo9Δ1
inviable cell at high temperaturespo9Δ20
normal cellular FPP levelspo9Δ1
normal meiosisspo9Δ149
normal protein localization to plasma membrane12
affecting rhb1spo9Δ
viable vegetative cellspo9Δ3651
viable vegetative cell with normal cell morphologyspo9Δ3100

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
viable vegetative cell populationfps1+, spo9Δ

Cell Phenotype

Term NameGenotypes
decreased farnesyltranstransferase activityspo9-R109Q (R109Q), fps1-R104Q (R104Q)
normal sporulationfps1+, spo9Δ
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons853592..853103, 853053..852916, 852847..852647, 852606..851808
Intron853102..853054, 852915..852848, 852646..852607
5' UTR853592..853238PMID:21511999
CDS853237..853103, 853053..852916, 852847..852647, 852606..852025
3' UTR852024..851808PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00348 Pfam IPR000092 Polyprenyl synthetase 48 307 4
PS00723 Prosite Patterns IPR000092 Polyprenyl synthetase 97 111 3
PS00444 Prosite Patterns IPR000092 Polyprenyl synthetase 231 243 3
PTHR11525 HMMPANTHER 11 351 2
1.10.600.10 Gene3D IPR008949 Isoprenoid synthase domain 13 350 5
SSF48576 SuperFamily IPR008949 Isoprenoid synthase domain 13 350 5

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.66 Da
Charge -5.50
Codon Adaptation Index 0.40
Isoelectric point 5.07
Molecular weight 40.95 kDa
Number of residues 351
Gene Expression

Qualitative Gene Expression

RNA leveldecreased during GO:0006995Northern assay evidencePMID:17596513
present during GO:0072690Northern assay evidencePMID:17596513

Quantitative Gene Expression

View graphical display of gene expression data for spo9 (SPBC36.06c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1118during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
956during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
475.63during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
783during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.25during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
functionally complemented by S. cerevisiae ERG20 PMID:17596513
functionally complements S. cerevisiae BTS1 PMID:17596513
functionally complemented by S. cerevisiae BTS1 PMID:17596513
Taxonomic Conservation
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC36.06c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withspo9farnesyl pyrophosphate synthetase Two-hybridPMID:17596513
binds activation domain construct withfps1geranyltranstransferase Fps1 Two-hybridPMID:17596513
binds DNA-binding domain construct withfps1geranyltranstransferase Fps1 Two-hybridPMID:26771498
affinity captured byfps1geranyltranstransferase Fps1 Affinity Capture-WesternPMID:17596513
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC36.06c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuestsc2tuberin Dosage RescuePMID:16624901
overexpression rescuescpp1protein farnesyltransferase beta subunit Cpp1 Dosage RescuePMID:16624901
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
rescued by overexpression offps1geranyltranstransferase Fps1 Dosage RescuePMID:17596513
External References
Database Identifier Description
NBRP SPBC36.06c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC36.06c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC36.06c BioGRID Interaction Datasets
Expression Viewer SPBC36.06c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC36.06c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC36.06c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC36.06c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC36.06c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC36.06c Transcriptome Viewer (Bähler Lab)
GEO SPBC36.06c GEO profiles
PInt SPBC36.06c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC36.06c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC36.06c Fission yeast phenotypic data & analysis
Cyclebase SPBC36.06c.1 Cell Cycle Data
IntEnz2.5.1.1Integrated relational Enzyme database
Rhea2.5.1.1Annotated reactions database
SPD / RIKEN22/22B05Orfeome Localization Data
UniProtKB/SwissProtO59703Geranylgeranyl pyrophosphate synthase
ModBaseO59703Database of comparative protein structure models
STRINGO59703Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595334farnesyl pyrophosphate synthetase
RefSeq mRNANM_001021242972h- farnesyl pyrophosphate synthetase (spo9), mRNA

Literature for spo9

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016