pmt3 (SPBC365.06)


Gene Standard Namepmt3 Characterisation Statuspublished
Systematic IDSPBC365.06 Feature Typeprotein coding
Synonymssmt3, ubl2 Name Descriptionswitching of mating type
ProductSUMO Product Size117aa, 12.93 kDa
Genomic Location Chromosome II, 2506815-2510260 (3446nt); CDS:2506894-2507390 (497nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding887
Annotation ExtensionEvidenceWith/FromReference
protein tag5
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
chromatin silencing at centromere58
Annotation ExtensionEvidenceWith/FromReference
chromatin silencing at silent mating-type cassette36
Annotation ExtensionEvidenceWith/FromReference
mitotic sister chromatid segregation187
Annotation ExtensionEvidenceWith/FromReference
negative regulation of histone H3-K14 acetylation2
Annotation ExtensionEvidenceWith/FromReference
negative regulation of histone H3-K4 methylation5
Annotation ExtensionEvidenceWith/FromReference
negative regulation of histone H3-K9 methylation1
Annotation ExtensionEvidenceWith/FromReference
protein sumoylation10
Annotation ExtensionEvidenceWith/FromReference
telomere maintenance45
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
linear element6
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body220
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
sensitive to cisplatinpmt3Δ106
sensitive to hydroxyureapmt3Δ594
viable vegetative cell populationpmt3Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclepmt3Δ894
abolished protein binding147
affecting cdc48 and pmt3SUMO-AA
affecting pmt3 and npl4SUMO-AA
cutpmt3Δ128
cut during cellular response to hydroxyureapmt3Δ44
decreased replication fork arrest at mating-type locuspmt3Δ5
decreased site-specific DNA replication termination at RTS1 barrierpmt3Δ2
elongated telomerespmt3Δ176
elongated vegetative cellpmt3Δ802
normal protein binding91
affecting ufd1 and pmt3SUMO-AA
normal protein level during vegetative growth91
affecting mei2pmt3Δ
viable elongated vegetative cellpmt3Δ325

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
normal vegetative cell population growthSUMO-K14R,K30R (K14R, K30R), slx8-29, ufd1Δ-Ct (336-342)
SUMO-K14R,K30R (K14R, K30R), slx8-29, ufd1-AAA (P337A, I338A, I340A)
slx8-29, SUMO-K14R,K30R (K14R, K30R)

Cell Phenotype

Term NameGenotypes
decreased cellular HMW SUMO conjugate levelpmt3+, nup132Δ
decreased protein processing
affecting pmt3nup132Δ, pmt3+
affecting pmt3slx8-29, pmt3+, nup132Δ
decreased replication fork arrest at mating-type locuspmt3Δ, rtf2Δ
decreased site-specific DNA replication termination at RTS1 barrierpmt3Δ, rtf2Δ
increased cellular HMW SUMO conjugate levelslx8-29, pmt3+, nup132Δ
pmt3+, slx8-29
increased level of large-Y replication intermediatespmt3Δ, rtf2Δ
increased level of sumoylated protein in cellpmt3+, slx8-29
slx8-29, pmt3+, nup132Δ
affecting pli1nup132Δ, SUMO-D18R (D18R)
affecting pli1nup132Δ, SUMO-K14R,K30R (K14R, K30R)
normal protein level during vegetative growth
affecting pli1nup132Δ, SUMO-K14R,K30R (K14R, K30R)
affecting pli1nup132Δ, SUMO-D18R (D18R)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in nup132 nucleoporin Nup132
FYPO affected by mutation in pmt3 SUMO
FYPO affected by mutation in slx1 structure-specific endonuclease catalytic subunit Slx1
FYPO affected by mutation in slx8 SUMO-targeted ubiquitin-protein ligase E3 Slx8
FYPO affected by mutation in ufd1 Hrd1 ubiquitin ligase complex subunit Ufd1 (predicted)
FYPO localization affected by mutation in hus5 SUMO conjugating enzyme E2 Hus5
FYPO localization affected by mutation in pli1 SUMO E3 ligase Pli1
FYPO localization affected by mutation in rec10 meiotic recombination protein Rec10
GO substrate of ulp1 SUMO deconjugating enzyme Ulp1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2506815..2507022, 2507117..2507266, 2507316..2510125, 2510164..2510260
Intron2507023..2507116, 2507267..2507315, 2510126..2510163
mRNA2506815..2510260
5' UTR2506815..2506893PMID:21511999
CDS2506894..2507022, 2507117..2507266, 2507316..2507390
3' UTR2507391..2510125, 2510164..2510260PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11976 Pfam IPR022617 Rad60/SUMO-like domain 36 106 2
SM00213 SMART IPR000626 Ubiquitin domain 36 107 13
PS50053 Prosite Profiles IPR000626 Ubiquitin domain 34 111 18
PTHR10562 HMMPANTHER 26 112 2
3.10.20.90 Gene3D Rho guanine nucleotide exchange factor Cdc24/Scd1 27 111 28
SSF54236 SuperFamily IPR029071 Ubiquitin-related domain 23 111 29

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001447ubiquitin family proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001447

Protein Properties

Ave. residue weight 110.55 Da
Charge -5.00
Codon Adaptation Index 0.50
Isoelectric point 4.59
Molecular weight 12.93 kDa
Number of residues 117
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS10
present during mitotic M phaseS25
present during mitotic M phaseS4
present during mitotic M phaseS15
present during mitotic M phaseS2
S2, S4, S10, S25
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S10 PMID:21712547
present during mitotic M phase experimental evidence S25 PMID:21712547
present during mitotic M phase experimental evidence S4 PMID:21712547
present during mitotic M phase experimental evidence S15 PMID:21712547
present during mitotic M phase experimental evidence S2 PMID:21712547
experimental evidence S2 PMID:24763107
experimental evidence S4 PMID:24763107
experimental evidence S10 PMID:24763107
IDA S25 PMID:25720772
O-phospho-L-threonine 1085
present during mitotic M phaseT20
present during mitotic M phaseT18
present during mitotic M phaseT24
T24
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T20 PMID:21712547
present during mitotic M phase experimental evidence T18 PMID:21712547
present during mitotic M phase experimental evidence T24 PMID:21712547
IDA T24 PMID:25720772
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
sumoylated lysine 174
Annotation ExtensionEvidenceResidueReference
IDA PMID:26404184
O-phosphorylated residueT18,S25, T18,T24 2457
Annotation ExtensionEvidenceResidueReference
IDA T18,T24 PMID:25720772
IDA T18,S25 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pmt3 (SPBC365.06)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
55597during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
66840during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
59282during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
58397during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
29295.21during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
56319during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
27901.42during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC365.06 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
modified byulp1SUMO deconjugating enzyme Ulp1 Biochemical ActivityPMID:24818994
modified byulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Biochemical ActivityPMID:24818994
affinity capturestif471translation initiation factor eIF4G Affinity Capture-WesternPMID:24818994
affinity capturestpz1Tppl homolog Tpz1 Affinity Capture-WesternPMID:24925530
affinity capturesucp6UBA domain protein Ucp6 Affinity Capture-MSPMID:22730331
affinity capturesSPCC16C4.106-phosphogluconolactonase (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPBP4H10.12conserved protein Affinity Capture-MSPMID:22730331
affinity capturesarc2ARP2/3 actin-organizing complex subunit Arc34 Affinity Capture-MSPMID:22730331
affinity capturestif6translation initiation factor-like ribosome biogenesis protein Affinity Capture-MSPMID:22730331
affinity capturesSPAC694.02DEAD/DEAH box helicase Affinity Capture-MSPMID:22730331
affinity capturesSPAC17H9.13cglutamate 5-kinase (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPAC5H10.03phosphoglycerate mutase family Affinity Capture-MSPMID:22730331
affinity capturesspp42U5 snRNP complex subunit Spp42 Affinity Capture-MSPMID:22730331
affinity capturesspt16FACT complex subunit Spt16 Affinity Capture-MSPMID:22730331
affinity capturestdp1tyrosyl-DNA phosphodiesterase Tdp1 Affinity Capture-MSPMID:22730331
affinity capturesrrp2ATP-dependent DNA helicase, ubiquitin-protein ligase E3 (predicted) Affinity Capture-MSPMID:22730331
affinity captureshtr12serine protease (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPCC1442.04cmeiotic recombination protein (predicted) Affinity Capture-MSPMID:22730331
affinity capturesnpl4Hrd1p ubiquitin ligase complex Npl4 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPBC2F12.05csterol binding ankyrin repeat protein (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPBC1778.07methyltransferase N2227 family Affinity Capture-MSPMID:22730331
affinity capturessts5RNB-like protein Affinity Capture-MSPMID:22730331
affinity capturesmtr1microtubule regulator Mtr1 Affinity Capture-MSPMID:22730331
affinity capturesSPAPB17E12.14c6-phosphofructo-2-kinase/fructose-2,6-bisphosphate 2-phosphatase (predicted) Affinity Capture-MSPMID:22730331
affinity capturesppe1serine/threonine protein phosphatase Ppe1 Affinity Capture-MSPMID:22730331
affinity captureseis1eisosome assembly protein eis1 Affinity Capture-MSPMID:22730331
affinity capturesSPAC12G12.07cconserved fungal protein Affinity Capture-MSPMID:22730331
affinity capturestma20RNA-binding protein Tma20 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesfbp1fructose-1,6-bisphosphatase Fbp1 Affinity Capture-MSPMID:22730331
affinity capturesprs1cytoplasmic proline-tRNA ligase Prs1 (predicted) Affinity Capture-MSPMID:22730331
affinity captureslrs1cytoplasmic leucine-tRNA ligase Lrs1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesrki1ribose 5-phosphate isomerase Rki1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesenp2rRNA processing protein Enp2 (predicted) Affinity Capture-MSPMID:22730331
affinity capturescms1U3-containing 90S preribosome complex subunit Cms1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesrex4exoribonuclease Rex4 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesegd2nascent polypeptide-associated complex alpha subunit Egd2 Affinity Capture-MSPMID:22730331
affinity capturesdrs1cytoplasmic aspartate-tRNA ligase Drs1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPBC713.07cvacuolar polyphosphatase (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPAC16C9.01ccarbohydrate kinase (predicted) Affinity Capture-MSPMID:22730331
affinity captureshhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:22730331
affinity capturesada1adenosine deaminase Ada1 Affinity Capture-MSPMID:22730331
affinity capturesrad13DNA repair nuclease Rad13 Affinity Capture-MSPMID:22730331
affinity capturespho4thiamine-repressible acid phosphatase Pho4 Affinity Capture-MSPMID:22730331
affinity capturesfum1fumarate hydratase (predicted) Affinity Capture-MSPMID:22730331
affinity captureslea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesisn1IMP 5'-nucleotidase Isn1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturessla1La protein homolog Affinity Capture-MSPMID:22730331
affinity capturesrpl370360S ribosomal protein L37 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesqrs1cytoplasmic glutaminyl-tRNA ligase Qrs1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesshm1serine hydroxymethyltransferase Shm1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturespda1pyruvate dehydrogenase e1 component alpha subunit Pda1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesgcs2glutamate-cysteine ligase regulatory subunit Gcs2 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPAC24B11.07coxidoreductase (predicted) Affinity Capture-MSPMID:22730331
affinity captureslys4homocitrate synthase Affinity Capture-MSPMID:22730331
affinity capturesSPAC6G10.09alpha glucosidase I Gls1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPAC144.05ATP-dependent DNA helicase/ ubiquitin-protein ligase E3 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesrpl230160S ribosomal protein L23 Affinity Capture-MSPMID:22730331
affinity capturesSPBC12C2.04NAD binding dehydrogenase family protein Affinity Capture-MSPMID:22730331
affinity capturespfl4cell surface glycoprotein, adhesion molecule (predicted) Affinity Capture-MSPMID:22730331
affinity capturesmpd1thioredoxin family protein Mpd1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesnup124nucleoporin Nup124 Affinity Capture-MSPMID:22730331
affinity capturesSPAC15A10.07Schizosaccharomyces specific protein Affinity Capture-MSPMID:22730331
affinity captureshsp78mitochondrial heatshock protein Hsp78 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesdnt1nucleolar protein Dnt1 Affinity Capture-MSPMID:22730331
affinity capturesSPAC3F10.16cGTP binding protein, HSR1-related (predicted) Affinity Capture-MSPMID:22730331
affinity capturesprs5ribose-phosphate pyrophosphokinase Prs5 (predicted) Affinity Capture-MSPMID:22730331
affinity capturestif33translation initiation factor eIF3c Affinity Capture-MSPMID:22730331
affinity capturesrpl4260S ribosomal protein L36/L42 Affinity Capture-MSPMID:22730331
affinity capturesosm1fumarate reductase Osm1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturespaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22730331
affinity capturesSPACUNK4.16calpha,alpha-trehalose-phosphate synthase (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPBP8B7.31acid phosphatase (predicted) Affinity Capture-MSPMID:22730331
affinity capturestps1alpha,alpha-trehalose-phosphate synthase [UDP-forming] Affinity Capture-MSPMID:22730331
affinity capturestxl1thioredoxin-like I protein Txl1 Affinity Capture-MSPMID:22730331
affinity capturesppp16serine-type peptidase activity Affinity Capture-MSPMID:22730331
affinity capturesSPAC1805.16cpurine nucleoside phosphorylase (predicted) Affinity Capture-MSPMID:22730331
affinity capturespir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:22730331
affinity capturestif32translation initiation factor eIF3a Affinity Capture-MSPMID:22730331
affinity capturesure7urease accessory protein UreG Affinity Capture-MSPMID:22730331
affinity capturesarc5ARP2/3 actin-organizing complex subunit Arc5 Affinity Capture-MSPMID:22730331
affinity capturesilv1acetolactate synthase catalytic subunit Affinity Capture-MSPMID:22730331
affinity capturesvtc4vacuolar transporter chaperone (VTC) complex subunit (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPBC1105.01rRNA processing protein Rrp12-like (predicted) Affinity Capture-MSPMID:22730331
affinity capturesatp12mitochondrial F1-ATPase chaperone Atp12 (predicted) Affinity Capture-MSPMID:22730331
affinity captureskap95karyopherin Kap95 Affinity Capture-MSPMID:22730331
affinity capturesgdi1GDP dissociation inhibitor Gdi1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturessds22protein phosphatase regulatory subunit Sds22 Affinity Capture-MSPMID:22730331
affinity capturesSPAC23A1.14cuncharacterised trans-sulfuration enzyme (predicted) Affinity Capture-MSPMID:22730331
affinity capturesthi4bifunctional thiamine-phosphate dipyrophosphorylase/hydroxyethylthiazole kinase Affinity Capture-MSPMID:22730331
affinity capturesadh8alcohol dehydrogenase (predicted) Affinity Capture-MSPMID:22730331
affinity capturesrps190140S ribosomal protein S19 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesfab11-phosphatidylinositol-3-phosphate 5-kinase Fab1 Affinity Capture-MSPMID:22730331
affinity capturesSPAC29E6.06ccytoplasmic cysteine-tRNA ligase Crs1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesarp3Arp2/3 protein complex, actin-like protein subunit Arp3 Affinity Capture-MSPMID:22730331
affinity capturesseb1Nrd1 comples RNA-binding subunit Seb1 Affinity Capture-MSPMID:22730331
affinity capturesSPBP23A10.11ccircularly permuted 1,3-beta-glucanase (predicted) Affinity Capture-MSPMID:22730331
affinity capturesdpe1dipeptidyl peptidase (predicted) Affinity Capture-MSPMID:22730331
affinity capturesmmf1mitochondrial matrix protein, YjgF family protein Mmf1 Affinity Capture-MSPMID:22730331
affinity capturesufd1Hrd1 ubiquitin ligase complex subunit Ufd1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturessds23PP2A-type phosphatase inhibitor Sds23/Moc1 Affinity Capture-MSPMID:22730331
affinity capturesSPAC25G10.01RNA-binding protein involved in histone acetylation Affinity Capture-MSPMID:22730331
affinity capturesbag101BAG family molecular chaperone regulator Bag101 (predicted) Affinity Capture-MSPMID:22730331
affinity capturestrp3anthranilate synthase component I (predicted) Affinity Capture-MSPMID:22730331
affinity capturesprp43ATP-dependent RNA helicase Prp43 Affinity Capture-MSPMID:22730331
affinity capturesSPCC622.15cSchizosaccharomyces specific protein Affinity Capture-MSPMID:22730331
affinity capturesusp105U1 snRNP-associated protein Usp105 Affinity Capture-MSPMID:22730331
affinity capturesarf1ADP-ribosylation factor, Arf family Arf1 Affinity Capture-MSPMID:22730331
affinity capturesnep1NEDD8 protease Nep1 Affinity Capture-MSPMID:22730331
affinity capturescdc37Hsp90 co-chaperone Cdc37 Affinity Capture-MSPMID:22730331
affinity capturesvip1RNA-binding protein Vip1 Affinity Capture-MSPMID:22730331
affinity capturesusp102U1 snRNP-associated protein Usp102 Affinity Capture-MSPMID:22730331
affinity capturesrps80140S ribosomal protein S8 (predicted) Affinity Capture-MSPMID:22730331
affinity captureshrs1mitochondrial and cytoplasmic histidine-tRNA ligase Hrs1 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesoca2serine/threonine protein kinase Oca2 Affinity Capture-MSPMID:22730331
affinity capturesnuc1DNA-directed RNA polymerase I complex large subunit Nuc1 Affinity Capture-MSPMID:22730331
affinity captureseif6translation initiation factor eIF3f Affinity Capture-MSPMID:22730331
affinity capturesdfr1dihydrofolate reductase/ serine hydrolase family fusion protein Dfr1 Affinity Capture-MSPMID:22730331
affinity capturescox4cytochrome c oxidase subunit IV (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPAC1002.12csuccinate-semialdehyde dehydrogenase [NAD(P)+] (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPCC1183.02glutathione S-transferase, translational elongation factor eEF1 (predicted) Affinity Capture-MSPMID:22730331
affinity captureshus5SUMO conjugating enzyme E2 Hus5 Affinity Capture-MSPMID:22730331
affinity capturesarc4ARP2/3 actin-organizing complex subunit Arc4 Affinity Capture-MSPMID:22730331
affinity capturesSPCC594.01DUF1769 family protein Affinity Capture-MSPMID:22730331
affinity capturesckb1CK2 family regulatory subunit Ckb1 Affinity Capture-MSPMID:22730331
affinity capturesSPAC13C5.04amidotransferase (predicted) Affinity Capture-MSPMID:22730331
affinity capturesrad31SUMO activating enzyme E1-type Rad31 Affinity Capture-MSPMID:22730331
affinity capturesrpp10160S acidic ribosomal protein A1 Affinity Capture-MSPMID:22730331
affinity capturesntp1alpha,alpha-trehalase Ntp1 Affinity Capture-MSPMID:22730331
affinity capturesrpl320260S ribosomal protein L32 (predicted) Affinity Capture-MSPMID:22730331
affinity captureshsp10mitochondrial heat shock protein Hsp10 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesuba2SUMO activating enzyme E1-type Uba2 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPBC4.03cCOPII-coated vesicle component Sfb3 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesSPCC1840.05cphosphoglucomutase (predicted) Affinity Capture-MSPMID:22730331
affinity capturesnda3tubulin beta Nda3 Affinity Capture-MSPMID:22730331
affinity capturesfps1geranyltranstransferase Fps1 Affinity Capture-MSPMID:22730331
affinity capturesmod5Tea1 anchoring protein Mod5 Affinity Capture-MSPMID:22730331
affinity capturesSPAC1142.02cTPR repeat protein, SGT2 family (predicted) Affinity Capture-MSPMID:22730331
affinity capturesrpl180260S ribosomal protein L18 (predicted) Affinity Capture-MSPMID:22730331
affinity capturesrpt319S proteasome regulatory subunit Rpt3 (predicted) Affinity Capture-MSPMID:22730331
affinity capturespob1Boi family protein Affinity Capture-MSPMID:22730331
affinity capturesgua2GMP synthase [glutamine-hydrolyzing] Gua2 (predicted) Affinity Capture-MSPMID:22730331
affinity capturestrp1anthranilate synthase component II, multifunctional enzyme Trp1 Affinity Capture-MSPMID:22730331
binds activation domain construct withrrp1ATP-dependent DNA helicase/ ubiquitin-protein ligase E3 (predicted) Two-hybridPMID:23828040
binds activation domain construct withrrp2ATP-dependent DNA helicase, ubiquitin-protein ligase E3 (predicted) Two-hybridPMID:23828040
binds activation domain construct withbrl1ubiquitin-protein ligase E3 Brl1 Two-hybridPMID:17762865
binds activation domain construct withbrl2ubiquitin-protein ligase E3 Brl2 Two-hybridPMID:17762865
binds activation domain construct withstn1telomere cap complex subunit Stn1 Two-hybridPMID:24711392
forms complex withhus5SUMO conjugating enzyme E2 Hus5 Reconstituted ComplexPMID:21444718
forms complex withrfp1SUMO-targeted ubiquitin-protein ligase subunit Rfp1 Reconstituted ComplexPMID:17762864
forms complex withslx1structure-specific endonuclease catalytic subunit Slx1 Reconstituted ComplexPMID:26787556
forms complex withufd1Hrd1 ubiquitin ligase complex subunit Ufd1 (predicted) Reconstituted ComplexPMID:22730331
binds DNA-binding domain construct withbrl2ubiquitin-protein ligase E3 Brl2 Two-hybridPMID:17762865
binds DNA-binding domain construct withrfp1SUMO-targeted ubiquitin-protein ligase subunit Rfp1 Two-hybridPMID:17762864
binds DNA-binding domain construct withhsp16heat shock protein Hsp16 Two-hybridPMID:11452028
binds DNA-binding domain construct withstn1telomere cap complex subunit Stn1 Two-hybridPMID:24925530
binds DNA-binding domain construct withufd1Hrd1 ubiquitin ligase complex subunit Ufd1 (predicted) Two-hybridPMID:22730331
affinity captured byufd1Hrd1 ubiquitin ligase complex subunit Ufd1 (predicted) Affinity Capture-WesternPMID:22730331
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC365.06 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetically rescuesslx8SUMO-targeted ubiquitin-protein ligase E3 Slx8 Synthetic RescuePMID:21444718
synthetically rescuesulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Synthetic RescuePMID:21444718
positive genetic interaction withvps35retromer complex subunit Vps35 Positive GeneticPMID:22681890
positive genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
synthetically rescued bytop1DNA topoisomerase I Synthetic RescuePMID:21444718
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
negative genetic interaction withbrc1BRCT domain protein Brc1 Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction withdad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
negative genetic interaction withelp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrad57RecA family ATPase Rad57/Rhp57 Negative GeneticPMID:22681890
negative genetic interaction withdam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
negative genetic interaction withnup132nucleoporin Nup132 Negative GeneticPMID:22681890
negative genetic interaction withrsc4RSC complex subunit Rsc4 Negative GeneticPMID:22681890
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
negative genetic interaction withcwf21complexed with Cdc5 protein Cwf21 Negative GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction withrad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withhrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspc34DASH complex subunit Spc34 Negative GeneticPMID:22681890
negative genetic interaction withdad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
negative genetic interaction withklp6kinesin-8 family plus-end microtubule motor Klp6 Negative GeneticPMID:22681890
negative genetic interaction withrad51RecA family recombinase Rad51/Rhp51 Negative GeneticPMID:22681890
negative genetic interaction withnrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
negative genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
negative genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcsn2COP9/signalosome complex subunit Csn2 Negative GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
negative genetic interaction withspc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
negative genetic interaction withpho8vacuolar membrane alkaline phosphatase (predicted) Negative GeneticPMID:22681890
synthetic growth defect withnse2Smc5-6 complex non-SMC subunit SUMO ligase subunit Nse2 Synthetic Growth DefectPMID:21444718
synthetic growth defect withrad60DNA repair protein Rad60 Synthetic Growth DefectPMID:21444718
synthetic growth defect withsrs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:18931302
synthetic growth defect withrhp14XP-A family homolog Rhp14 Synthetic Growth DefectPMID:18931302
synthetic growth defect withslm9hira protein Slm9 Synthetic Growth DefectPMID:18931302
rescued byulp1SUMO deconjugating enzyme Ulp1 Phenotypic SuppressionPMID:24818994
synthetic lethal withrad51RecA family recombinase Rad51/Rhp51 Synthetic LethalityPMID:21444718
External References
Database Identifier Description
NBRP SPBC365.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC365.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC365.06 BioGRID Interaction Datasets
Expression Viewer SPBC365.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC365.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC365.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC365.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC365.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC365.06 Transcriptome Viewer (Bähler Lab)
GEO SPBC365.06 GEO profiles
PInt SPBC365.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC365.06 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC365.06 Fission yeast phenotypic data & analysis
Cyclebase SPBC365.06.1 Cell Cycle Data
SPD / RIKEN31/31E04Orfeome Localization Data
UniProtKB/SwissProtO13351Ubiquitin-like protein pmt3/smt3
ModBaseO13351Database of comparative protein structure models
STRINGO13351Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596035ubiquitin-like protein
RefSeq mRNANM_001021945972h- ubiquitin-like protein (pmt3), mRNA
European Nucleotide ArchiveAF019235ENA EMBL mapping
European Nucleotide ArchiveBAA32595.1ENA Protein Mapping
European Nucleotide ArchiveCAB44758.1ENA Protein Mapping
UniParcUPI0000135AEAUniProt Archive

Literature for pmt3

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Release Version: PomBase:30_61 - 12 Sep 2016