alp4 (SPBC365.15)


Gene Standard Namealp4 Characterisation Statuspublished
Systematic IDSPBC365.15 Feature Typeprotein coding
Synonyms Name Description
Productgamma tubulin complex Spc97/GCP2 subunit Alp4 Product Size784aa, 90.16 kDa
Genomic Location Chromosome II, 2523969-2527134 (3166nt); CDS:2524061-2526415 (2355nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding887
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
astral microtubule anchoring at mitotic spindle pole body1
Annotation ExtensionEvidenceWith/FromReference
cytoplasmic microtubule organization17
Annotation ExtensionEvidenceWith/FromReference
establishment or maintenance of cell polarity regulating cell shape55
Annotation ExtensionEvidenceWith/FromReference
microtubule nucleation11
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle assembly18
Annotation ExtensionEvidenceWith/FromReference
nuclear migration by microtubule mediated pushing forces1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site317
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
equatorial microtubule organizing center11
Annotation ExtensionEvidenceWith/FromReference
gamma-tubulin complex8
Annotation ExtensionEvidenceWith/FromReference
interphase microtubule organizing center7
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body220
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationalp4+ (wild type)1455
alp4Δ
sensitive to thiabendazolealp4-1891329

Cell Phenotype

Term NameGenotypesCount
abnormal cell cycle arrest in mitotic M phasealp4-189132
NLS-Alp4C (nuclear)
abnormal exit from mitosisalp4-18913
abnormal gamma-tubulin complex localization to mitotic spindle pole body following normal mitosisalp4-18911
abnormal microtubule cytoskeleton organization during vegetative growthalp4C-OP (1-565)295
abnormal mitotic sister chromatid segregationalp4+ (wild type)281
alp4-C(566-784) (1-565)
alp4-1891
NLS-Alp4C (nuclear)
abnormal mitotic spindle assemblyNLS-Alp4C (nuclear)19
abnormal mitotic spindle elongationNLS-Alp4C (nuclear)25
abolished mitotic cell cycle spindle assembly checkpointalp4-18919
astral microtubules present in decreased numbersalp4-18911
bent vegetative cellalp4-22511
cell cycle arrest in mitotic G2 phasealp4-189139
NLS-Alp4C (nuclear)
curved vegetative cellalp4+ (wild type)120
alp4-N(1-566) (556-784)
alp4-C(566-784) (1-565)
cutalp4-225128
alp4-1891
cytoplasmic microtubules nucleated from equatorial microtubule organizing center present in decreased numbersalp4-18911
decreased asymmetric protein localization to old or new mitotic spindle pole bodyalp4-189112
decreased asymmetric protein localization to old or new mitotic spindle pole body during anaphase, with protein symmetrically localized to both spindle pole bodies11
affects localization of sid1alp4-1891
affects localization of cdc7alp4-1891
decreased gamma-tubulin complex localization to mitotic spindle pole bodyNLS-Alp4C (nuclear)1
decreased protein localization to cell tip28
affects localization of bud6alp4-C(566-784) (1-565)
affects localization of bud6NES-Alp4C (cytoplasmic)
decreased protein localization to equatorial microtubule organizing center during vegetative growth1
affects localization of gtb1alp4-C(566-784) (1-565)
decreased protein localization to mitotic spindle pole body22
affects localization of gtb1alp4-C(566-784) (1-565)
decreased rate of microtubule depolymerization behind moving spindle pole bodyalp4C-OP (1-565)1
elongated monoseptate vegetative cellalp4-N(1-566) (556-784)7
alp4+ (wild type)
alp4-C(566-784) (1-565)
elongated septated vegetative cellNLS-Alp4C (nuclear)108
elongated vegetative cellalp4-1891802
elongated vegetative cell with elongated oscillating nucleusalp4C-OP (1-565)2
NES-Alp4C (cytoplasmic)
alp4-1891
alp4-N(1-566) (556-784)
alp4+ (wild type)
elongated vegetative cell with oscillating nucleusalp4-18912
increased cellular pigment accumulationalp4Δ31
increased duration of protein localization to mitotic spindle pole body8
affects localization of cdc13alp4-1891
increased protein localization to cell tip10
affects localization of tea1alp4-C(566-784) (1-565)
affects localization of tea1NES-Alp4C (cytoplasmic)
increased protein localization to non-growing cell tip3
affects localization of tip1alp4C-OP (1-565)
increased rate of microtubule depolymerization ahead of moving spindle pole bodyalp4C-OP (1-565)1
inviable curved vegetative cellalp4-189138
alp4Δ
inviable elongated aseptate cellalp4-N(1-566) (556-784)59
alp4+ (wild type)
alp4-C(566-784) (1-565)
inviable elongated mononucleate vegetative cell with mislocalized nucleusalp4-C(566-784) (1-565)2
inviable vegetative cellalp4Δ1-238 (1-238)1358
alp4-1891
long curved cytoplasmic microtubulesalp4-C(566-784) (1-565)6
alp4-1891
long cytoplasmic microtubulesalp4+ (wild type)26
mitotic spindle assembly checkpoint overridealp4-18912
monopolar mitotic spindlealp4-189110
multiseptate vegetative cellalp4-1891105
multiseptate vegetative cell, septa groupedalp4-189110
normal centromere clustering at nuclear peripheryalp4C-OP (1-565)7
normal mitotic cell cycle spindle assembly checkpointalp4-18915
normal mitotic spindle assemblyalp4-C(566-784) (1-565)2
normal protein localization to cell tip24
affecting bud6NLS-Alp4C (nuclear)
affecting tea1NLS-Alp4C (nuclear)
affecting tea1alp4-1891
normal telomere localization during mitotic interphasealp4C-OP (1-565)1
normal vegetative cell growthNES-Alp4C (cytoplasmic)11
normal vegetative cell lengthNES-Alp4C (cytoplasmic)10
nuclear envelope protrusion present during mitosisalp4Δ1-238 (1-238)8
short cytoplasmic microtubulesalp4-189120
short cytoplasmic microtubules present in increased numbersalp4-18912
spindle pole body detached from cytoplasmic microtubulesalp4-18912

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased vegetative cell population growthalp4-1891, cut7-22, gtb1-PL302
normal vegetative cell population growthcut7-22, alp4-1891

Cell Phenotype

Term NameGenotypes
elongated vegetative cell with elongated oscillating nucleusalp4C-OP (1-565), dhc1Δ, dlc1Δ
pkl1Δ, alp4C-OP (1-565), dhc1Δ
alp4C-OP (1-565), dhc1Δ, klp2Δ
alp4C-OP (1-565), klp2Δ
alp4C-OP (1-565), dhc1Δ
alp4C-OP (1-565), dlc1Δ
alp4C-OP (1-565), klp2Δ, pkl1Δ, dhc1Δ
pkl1Δ, alp4C-OP (1-565)
increased duration of protein localization to mitotic spindle pole body
affects localization of cdc13cdc25-22 (C532Y), alp4-C(566-784) (1-565)
inviable elongated mononucleate aseptate vegetative cell with cell cycle arrest in mitotic G2 phasecdc25-22 (C532Y), alp4-C(566-784) (1-565)
normal microtubule cytoskeleton organizationalp4Δ, tip1Δ
small vegetative cellwee1-50, alp4-C(566-784) (1-565)
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in alp6 gamma tubulin complex Spc98/GCP3 subunit Alp6
FYPO localization affected by mutation in cut11 spindle pole body docking protein Cut11
FYPO localization affected by mutation in mzt1 mitotic spindle organizing protein Mzt1
FYPO localization affected by mutation in tts1 tetra spanning protein 1, Tts1
GO localized by mcp6 horsetail movement protein Hrs1/Mcp6
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2523969..2527134
mRNA2523969..2527134
5' UTR2523969..2524060PMID:21511999
CDS2524061..2526415
3' UTR2526416..2527134PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04130 Pfam IPR007259 Gamma-tubulin complex component protein 74 693 4
PTHR19302:SF13 HMMPANTHER 242 526 1
PTHR19302:SF13 HMMPANTHER 560 782 1
PTHR19302:SF13 HMMPANTHER 60 207 1
PTHR19302 HMMPANTHER IPR007259 Gamma-tubulin complex component protein 60 207 3
PTHR19302 HMMPANTHER IPR007259 Gamma-tubulin complex component protein 560 782 3
PTHR19302 HMMPANTHER IPR007259 Gamma-tubulin complex component protein 242 526 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 115.00 Da
Charge -12.00
Codon Adaptation Index 0.39
Isoelectric point 5.45
Molecular weight 90.16 kDa
Number of residues 784
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS224
present during cellular response to thiabendazole, mitotic M phaseS39
present during mitotic M phaseS6
present during mitotic M phaseS4
present during mitotic M phaseS240
present during mitotic M phaseS18
present during mitotic M phaseS7
present during mitotic M phaseS35
present during mitotic M phaseS14
S4, S6, S7, S39
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S224 PMID:21712547
present during mitotic M phase experimental evidence S39 PMID:21712547
present during cellular response to thiabendazole IDA S39 PMID:18257517
present during mitotic M phase experimental evidence S6 PMID:21712547
present during mitotic M phase experimental evidence S4 PMID:21712547
present during mitotic M phase experimental evidence S240 PMID:21712547
present during mitotic M phase experimental evidence S18 PMID:21712547
present during mitotic M phase experimental evidence S7 PMID:21712547
present during mitotic M phase experimental evidence S35 PMID:21712547
present during mitotic M phase experimental evidence S14 PMID:21712547
experimental evidence S4 PMID:24763107
experimental evidence S7 PMID:24763107
experimental evidence S6 PMID:24763107
IDA S39 PMID:25720772
O-phosphorylated residueS14,S18 2457
Annotation ExtensionEvidenceResidueReference
IDA S14,S18 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for alp4 (SPBC365.15)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
31.67during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
921during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.58during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC365.15 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypkl1minus-end directed microtubule motor, kinesin Pkl1 Affinity Capture-WesternPMID:23388459
affinity captured bygfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:21444751
affinity captured bymto1MT organizer Mto1 Affinity Capture-WesternPMID:15004232
affinity captured byalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Affinity Capture-MSPMID:15004232
affinity captured bymod21gamma tubulin complex subunit Mod21 Affinity Capture-WesternPMID:21444751
affinity captured byalp16gamma tubulin complex subunit Alp16 Affinity Capture-WesternPMID:21444751
affinity captured bymzt1mitotic spindle organizing protein Mzt1 Affinity Capture-WesternPMID:23885124
binds DNA-binding domain construct withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Two-hybridPMID:16855399
binds DNA-binding domain construct withhrs1mitochondrial and cytoplasmic histidine-tRNA ligase Hrs1 (predicted) Two-hybridPMID:22425159
binds DNA-binding domain construct withcaf4CCR4-Not complex subunit Caf4/Mdv1 (predicted) Two-hybridPMID:26771498
binds DNA-binding domain construct withmsd1microtubule-anchoring factor Msd1 Two-hybridPMID:25987607
affinity capturesmto1MT organizer Mto1 Affinity Capture-WesternPMID:15120067
affinity capturesalp16gamma tubulin complex subunit Alp16 Affinity Capture-WesternPMID:17021256
affinity capturesmod21gamma tubulin complex subunit Mod21 Affinity Capture-WesternPMID:17021256
affinity capturesgtb1gamma-tubulin Gtb1 Affinity Capture-MSPMID:15004232
affinity capturesgfh1gamma tubulin complex subunit Gfh1 Affinity Capture-MSPMID:15004232
affinity capturespcp1pericentrin Pcp1 Affinity Capture-WesternPMID:15004232
affinity capturesmad2mitotic spindle checkpoint protein Mad2 Affinity Capture-WesternPMID:16855399
binds activation domain construct withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Two-hybridPMID:16855399
binds activation domain construct withgtb1gamma-tubulin Gtb1 Two-hybridPMID:16855399
binds activation domain construct withmad2mitotic spindle checkpoint protein Mad2 Two-hybridPMID:16855399
co-fractionates withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Co-fractionationPMID:23886939
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC365.15 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofalp16gamma tubulin complex subunit Alp16 Dosage RescuePMID:12134075
synthetic lethal withalp16gamma tubulin complex subunit Alp16 Synthetic LethalityPMID:12134075
synthetically rescuescut7kinesin family plus-end microtubule motor Cut7 Synthetic RescuePMID:18418055
phenotype enhanced bymad2mitotic spindle checkpoint protein Mad2 Phenotypic EnhancementPMID:11080156
synthetically rescued bygtb1gamma-tubulin Gtb1 Synthetic RescuePMID:15280226
overexpression rescuesalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Dosage RescuePMID:11080156
synthetic growth defect withmad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:11080156
External References
Database Identifier Description
NBRP SPBC365.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC365.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC365.15 BioGRID Interaction Datasets
Expression Viewer SPBC365.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC365.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC365.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC365.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC365.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC365.15 Transcriptome Viewer (Bähler Lab)
GEO SPBC365.15 GEO profiles
PInt SPBC365.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC365.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC365.15 Fission yeast phenotypic data & analysis
Cyclebase SPBC365.15.1 Cell Cycle Data
SPD / RIKEN38/38C08Orfeome Localization Data
UniProtKB/SwissProtQ9Y705Spindle pole body component alp4
ModBaseQ9Y705Database of comparative protein structure models
STRINGQ9Y705Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596044gamma tubulin complex Spc97/GCP2 subunit Alp4
RefSeq mRNANM_001021954972h- gamma tubulin complex Spc97/GCP2 subunit Alp4 (alp4), mRNA
European Nucleotide ArchiveBAA77269.1ENA Protein Mapping
European Nucleotide ArchiveCAB44767.1ENA Protein Mapping
UniParcUPI00001258B7UniProt Archive

Literature for alp4

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016