alp4 (SPBC365.15)


Gene Standard Namealp4 Characterisation Statuspublished
Systematic IDSPBC365.15 Feature Typeprotein coding
Synonyms Name Description
Productgamma tubulin complex Spc97/GCP2 subunit Alp4 Product Size784aa, 90.16 kDa
Genomic Location Chromosome II, 2523969-2527134 (3166nt); CDS:2524061-2526415 (2355nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPImto2PMID:19001497861
IPImto1PMID:19001497
IPImsd1PMID:17486116
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031122cytoplasmic microtubule organizationIMPPMID:1108015612
GO:0071963establishment or maintenance of cell polarity regulating cell shapeIMPPMID:965816937
during mitotic interphaseIMPPMID:16611237
GO:0033566gamma-tubulin complex localizationIMPPMID:166112376
GO:0034631microtubule anchoring at spindle pole bodyIMPPMID:174861162
GO:0007020microtubule nucleationICGO:0000930GO_REF:000000112
GO:0000281mitotic cytokinesisIMPPMID:1195283368
GO:0007094mitotic spindle assembly checkpointIMPPMID:1108015627
GO:0030473nuclear migration along microtubuleIMPPMID:1661123716
GO:0090307spindle assembly involved in mitosisIMPPMID:1108015617
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372295
GO:0005829cytosolIDAPMID:168233722316
GO:0000923equatorial microtubule organizing centerIDAPMID:1108015611
GO:0000930gamma-tubulin complexTASPMID:110801568
GO:0044732mitotic spindle pole bodyIDAPMID:16823372214
IDAPMID:11080156
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyalp4ΔNullPECO:0000005, PECO:0000137PMID:236978061315
Microscopyalp4ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003165cutnot recorded (unrecorded)PMID:1108015680
FYPO:0000741increased cellular pigment accumulationMicroscopyalp4ΔNullPECO:0000005, PECO:0000137PMID:2369780623
FYPO:0002113inviable curved vegetative cell31
penetrance FYPO_EXT:0000003Microscopyalp4ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000055long microtubulesnot recorded (unrecorded)PECO:0000004PMID:110801563
FYPO:0000276monopolar mitotic spindlenot recorded (unrecorded)PECO:0000004PMID:110801565
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in alp6 gamma tubulin complex Spc98/GCP3 subunit Alp6 PMID:23885124
FYPO affected by mutation in mzt1 mitotic spindle organizing protein Mzt1 PMID:23885124
GO localized by mcp6 horsetail movement protein Hrs1/Mcp6 PMID:16111942
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
125239692527134

UTRs

Region Coordinates Reference
five_prime_UTR2523969..2524060PMID:21511999
three_prime_UTR2526416..2527134PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04130 Pfam IPR007259 Gamma-tubulin complex component protein 74 693 4
PTHR19302:SF13 HMMPANTHER 60 782 1
PTHR19302 HMMPANTHER IPR007259 Gamma-tubulin complex component protein 60 782 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 115.00 Da
Charge -12.00
Isoelectric point 5.45
Molecular weight 90.16 kDa
Number of residues 784
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS6PMID:217125471663
present during mitotic M phase
present during mitotic M phaseS224PMID:21712547
present during mitotic M phaseS18PMID:21712547
present during mitotic M phaseS240PMID:21712547
present during mitotic M phaseS4PMID:21712547
present during mitotic M phaseS14PMID:21712547
present during cellular response to thiabendazoleIDAS39PMID:18257517
present during mitotic M phaseS7PMID:21712547
S7PMID:24763107
S6PMID:24763107
S4PMID:24763107
present during mitotic M phaseS39PMID:21712547
present during mitotic M phaseS35PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171915
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
31.67during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
921during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.58during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
mad2spindle checkpoint protein Mad2 Phenotypic EnhancementPMID:11080156
Synthetic Growth Defect
alp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Dosage RescuePMID:11080156
alp16gamma tubulin complex subunit Alp16 Dosage RescuePMID:12134075
Synthetic Lethality
gtb1gamma-tubulin Gtb1 Synthetic RescuePMID:15280226
cut7kinesin-like protein Cut7 Synthetic RescuePMID:18418055
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hrs1mitochondrial and cytoplasmic histidine-tRNA ligase Hrs1 (predicted) Two-hybridPMID:22425159
gtb1gamma-tubulin Gtb1 Affinity Capture-WesternPMID:12134075
Affinity Capture-WesternPMID:11080156
Affinity Capture-MSPMID:15004232
Two-hybridPMID:16855399
pcp1pericentrin Pcp1 Affinity Capture-WesternPMID:15004232
gfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:21444751
Affinity Capture-WesternPMID:15004232
Affinity Capture-MS
Affinity Capture-WesternPMID:17021256
pkl1kinesin-like protein Pkl1 Affinity Capture-WesternPMID:23388459
mzt1mitotic spindle organizing protein Mzt1 Affinity Capture-WesternPMID:23885124
mod21gamma tubulin complex subunit Mod21 Affinity Capture-WesternPMID:21444751
Affinity Capture-WesternPMID:17021256
mad2spindle checkpoint protein Mad2 Affinity Capture-WesternPMID:16855399
Two-hybrid
alp16gamma tubulin complex subunit Alp16 Affinity Capture-WesternPMID:21444751
Affinity Capture-MSPMID:15004232
Affinity Capture-WesternPMID:17021256
alp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Affinity Capture-MSPMID:15004232
Two-hybridPMID:24006493
Two-hybridPMID:16855399
Co-fractionationPMID:23886939
mto1MT organizer Mto1 Affinity Capture-WesternPMID:15659644
Affinity Capture-WesternPMID:15120067
Affinity Capture-WesternPMID:15004232
Affinity Capture-MS
External References
Database Identifier Description
NBRP SPBC365.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC365.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC365.15 BioGRID Interaction Datasets
Expression Viewer SPBC365.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC365.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC365.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC365.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC365.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC365.15 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC365.15 Cell Cycle Data
GEO SPBC365.15 GEO profiles
PInt SPBC365.15 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2540392gamma tubulin complex Spc97/GCP2 subunit Alp4
WikiGene2540392gamma tubulin complex Spc97/GCP2 subunit Alp4
SPD / RIKEN38/38C08Orfeome Localization Data
UniProtKB/SwissProtQ9Y705Spindle pole body component alp4
ModBaseQ9Y705Database of comparative protein structure models
STRINGQ9Y705Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596044gamma tubulin complex Spc97/GCP2 subunit Alp4
RefSeq mRNANM_001021954972h- gamma tubulin complex Spc97/GCP2 subunit Alp4 (alp4), mRNA
European Nucleotide ArchiveBAA77269ENA Protein Mapping
European Nucleotide ArchiveBAA77269.1ENA Protein Mapping
European Nucleotide ArchiveCAB44767ENA Protein Mapping
European Nucleotide ArchiveCAB44767.1ENA Protein Mapping
UniParcUPI00001258B7UniProt Archive

Literature for alp4

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014