alp4 (SPBC365.15)


Gene Standard Namealp4 Characterisation Statuspublished
Systematic IDSPBC365.15 Feature Typeprotein coding
Synonyms Name Description
Productgamma tubulin complex Spc97/GCP2 subunit Alp4 Product Size784aa, 90.16 kDa
Genomic Location Chromosome II, 2523969-2527134 (3166nt); CDS:2524061-2526415 (2355nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding851
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic microtubule organization11
Annotation ExtensionEvidenceWith/FromReference
establishment or maintenance of cell polarity regulating cell shape57
Annotation ExtensionEvidenceWith/FromReference
gamma-tubulin complex localization6
Annotation ExtensionEvidenceWith/FromReference
microtubule anchoring at spindle pole body2
Annotation ExtensionEvidenceWith/FromReference
microtubule nucleation11
Annotation ExtensionEvidenceWith/FromReference
mitotic cytokinesis122
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle assembly17
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle assembly checkpoint13
Annotation ExtensionEvidenceWith/FromReference
nuclear migration along microtubule15
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site300
Annotation ExtensionEvidenceWith/FromReference
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
equatorial microtubule organizing center11
Annotation ExtensionEvidenceWith/FromReference
gamma-tubulin complex8
Annotation ExtensionEvidenceWith/FromReference
interphase microtubule organizing center7
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body216
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationalp4ΔNull1428
unnamed (wild type)

Cell Phenotype

Term NameAlleleExpressionCount
cutunnamed (unrecorded)94
increased cellular pigment accumulationalp4ΔNull23
inviable curved vegetative cellalp4ΔNull32
inviable vegetative cellalp41238 (1-238)Endogenous1271
long microtubulesunnamed (unrecorded)25
monopolar mitotic spindleunnamed (unrecorded)6
nuclear envelope protrusion present during mitosisalp41238 (1-238)Endogenous5
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in alp6 gamma tubulin complex Spc98/GCP3 subunit Alp6 PMID:23885124
FYPO affected by mutation in cut11 spindle pole body docking protein Cut11 PMID:25103238
FYPO affected by mutation in mzt1 mitotic spindle organizing protein Mzt1 PMID:23885124
GO localized by mcp6 horsetail movement protein Hrs1/Mcp6 PMID:16111942
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
125239692527134

UTRs

Region Coordinates Reference
five_prime_UTR2523969..2524060PMID:21511999
three_prime_UTR2526416..2527134PMID:21511999
mRNA2523969..2527134
exon2524061..2526415
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04130 Pfam IPR007259 Gamma-tubulin complex component protein 74 693 4

View domain organization at Pfam

Protein Properties

Ave. residue weight 115.00 Da
Charge -12.00
Isoelectric point 5.45
Molecular weight 90.16 kDa
Number of residues 784
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS14
S4, S6, S7
present during mitotic M phase, cellular response to thiabendazoleS39
present during mitotic M phaseS7
present during mitotic M phaseS35
present during mitotic M phaseS6
present during mitotic M phaseS224
present during mitotic M phaseS18
present during mitotic M phaseS240
present during mitotic M phaseS4
Annotation ExtensionEvidenceResidueReference
experimental evidence S4 PMID:24763107
present during mitotic M phase experimental evidence S4 PMID:21712547
experimental evidence S6 PMID:24763107
present during mitotic M phase experimental evidence S6 PMID:21712547
experimental evidence S7 PMID:24763107
present during mitotic M phase experimental evidence S7 PMID:21712547
present during mitotic M phase experimental evidence S14 PMID:21712547
present during mitotic M phase experimental evidence S18 PMID:21712547
present during mitotic M phase experimental evidence S35 PMID:21712547
present during cellular response to thiabendazole IDA S39 PMID:18257517
present during mitotic M phase experimental evidence S39 PMID:21712547
present during mitotic M phase experimental evidence S224 PMID:21712547
present during mitotic M phase experimental evidence S240 PMID:21712547
phosphorylated residue 1923
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
921during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
31.67during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.58during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byalp16gamma tubulin complex subunit Alp16 Affinity Capture-WesternPMID:21444751
affinity capturesalp16gamma tubulin complex subunit Alp16 Affinity Capture-MSPMID:15004232
affinity capturesalp16gamma tubulin complex subunit Alp16 Affinity Capture-WesternPMID:17021256
affinity captured byalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Affinity Capture-MSPMID:15004232
binds DNA-binding domain construct withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Two-hybridPMID:24006493
binds activation domain construct withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Two-hybridPMID:16855399
binds DNA-binding domain construct withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Two-hybridPMID:16855399
co-fractionates withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Co-fractionationPMID:23886939
affinity captured bygfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:21444751
affinity captured bygfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:15004232
affinity capturesgfh1gamma tubulin complex subunit Gfh1 Affinity Capture-MSPMID:15004232
affinity capturesgfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:15004232
affinity capturesgfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:17021256
affinity capturesgtb1gamma-tubulin Gtb1 Affinity Capture-WesternPMID:12134075
affinity capturesgtb1gamma-tubulin Gtb1 Affinity Capture-WesternPMID:11080156
affinity capturesgtb1gamma-tubulin Gtb1 Affinity Capture-MSPMID:15004232
binds activation domain construct withgtb1gamma-tubulin Gtb1 Two-hybridPMID:16855399
binds DNA-binding domain construct withhrs1mitochondrial and cytoplasmic histidine-tRNA ligase Hrs1 (predicted) Two-hybridPMID:22425159
affinity capturesmad2mitotic spindle checkpoint protein Mad2 Affinity Capture-WesternPMID:16855399
binds activation domain construct withmad2mitotic spindle checkpoint protein Mad2 Two-hybridPMID:16855399
affinity captured bymod21gamma tubulin complex subunit Mod21 Affinity Capture-WesternPMID:21444751
affinity capturesmod21gamma tubulin complex subunit Mod21 Affinity Capture-WesternPMID:17021256
affinity captured bymto1MT organizer Mto1 Affinity Capture-WesternPMID:15659644
affinity capturesmto1MT organizer Mto1 Affinity Capture-WesternPMID:15120067
affinity captured bymto1MT organizer Mto1 Affinity Capture-WesternPMID:15120067
affinity captured bymto1MT organizer Mto1 Affinity Capture-WesternPMID:15004232
affinity capturesmto1MT organizer Mto1 Affinity Capture-WesternPMID:15004232
affinity capturesmto1MT organizer Mto1 Affinity Capture-MSPMID:15004232
affinity captured bymzt1mitotic spindle organizing protein Mzt1 Affinity Capture-WesternPMID:23885124
affinity capturespcp1pericentrin Pcp1 Affinity Capture-WesternPMID:15004232
affinity captured bypkl1kinesin-like protein Pkl1 Affinity Capture-WesternPMID:23388459
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofalp16gamma tubulin complex subunit Alp16 Dosage RescuePMID:12134075
synthetic lethal withalp16gamma tubulin complex subunit Alp16 Synthetic LethalityPMID:12134075
overexpression rescuesalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Dosage RescuePMID:11080156
synthetically rescuescut7kinesin-like protein Cut7 Synthetic RescuePMID:18418055
synthetically rescued bygtb1gamma-tubulin Gtb1 Synthetic RescuePMID:15280226
synthetic growth defect withmad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:11080156
phenotype enhanced bymad2mitotic spindle checkpoint protein Mad2 Phenotypic EnhancementPMID:11080156
External References
Database Identifier Description
NBRP SPBC365.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC365.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC365.15 BioGRID Interaction Datasets
Expression Viewer SPBC365.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC365.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC365.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC365.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC365.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC365.15 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC365.15 Cell Cycle Data
GEO SPBC365.15 GEO profiles
PInt SPBC365.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC365.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC365.15 Fission yeast phenotypic data & analysis
SPD / RIKEN38/38C08Orfeome Localization Data
UniProtKB/SwissProtQ9Y705Spindle pole body component alp4
ModBaseQ9Y705Database of comparative protein structure models
STRINGQ9Y705Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596044gamma tubulin complex Spc97/GCP2 subunit Alp4
RefSeq mRNANM_001021954972h- gamma tubulin complex Spc97/GCP2 subunit Alp4 (alp4), mRNA
European Nucleotide ArchiveBAA77269.1ENA Protein Mapping
European Nucleotide ArchiveCAB44767.1ENA Protein Mapping
UniParcUPI00001258B7UniProt Archive

Literature for alp4

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015