alp4 (SPBC365.15)


Gene Standard Namealp4 Characterisation Statuspublished
Systematic IDSPBC365.15 Feature Typeprotein coding
Synonyms Name Description
Productgamma tubulin complex Spc97/GCP2 subunit Alp4 Product Size784aa, 90.16 kDa
Genomic Location Chromosome II, 2523969-2527134 (3166nt); CDS:2524061-2526415 (2355nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding859
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic microtubule organization10
Annotation ExtensionEvidenceWith/FromReference
establishment or maintenance of cell polarity regulating cell shape56
Annotation ExtensionEvidenceWith/FromReference
gamma-tubulin complex localization6
Annotation ExtensionEvidenceWith/FromReference
microtubule anchoring at spindle pole body2
Annotation ExtensionEvidenceWith/FromReference
microtubule nucleation11
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle assembly16
Annotation ExtensionEvidenceWith/FromReference
nuclear migration along microtubule15
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
equatorial microtubule organizing center11
Annotation ExtensionEvidenceWith/FromReference
gamma-tubulin complex8
Annotation ExtensionEvidenceWith/FromReference
interphase microtubule organizing center7
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body216
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationalp4+ (wild type)Overexpression1438
alp4ΔNull
sensitive to thiabendazolealp4-1891Not specified219

Cell Phenotype

Term NameAlleleExpressionCount
abnormal exit from mitosisalp4-1891Not specified2
abnormal gamma-tubulin complex localization to mitotic spindle pole body following normal mitosisalp4-1891Not specified1
abolished mitotic cell cycle spindle assembly checkpointalp4-1891Not specified3
cutalp4-1891Not specified96
decreased asymmetric protein localization to old or new mitotic spindle pole bodyalp4-1891Not specified12
decreased asymmetric protein localization to old or new mitotic spindle pole body during anaphase, with protein symmetrically localized to both spindle pole bodies11
affecting sid1alp4-1891Not specified
affecting cdc7alp4-1891Not specified
increased cellular pigment accumulationalp4ΔNull23
increased duration of protein localization to mitotic spindle pole body4
affecting cdc13alp4-1891Not specified
inviable curved vegetative cellalp4-1891Not specified36
alp4ΔNull
inviable vegetative cellalp4-1891Not specified1258
alp41238 (1-238)Endogenous
long curved cytoplasmic microtubulesalp4-1891Not specified3
long cytoplasmic microtubulesalp4+ (wild type)Overexpression22
mitotic spindle assembly checkpoint overridealp4-1891Not specified2
monopolar mitotic spindlealp4-1891Not specified6
multiseptate vegetative cellalp4-1891Not specified81
multiseptate vegetative cell, septa groupedalp4-1891Not specified4
normal mitotic cell cycle spindle assembly checkpointalp4-1891Not specified4
normal protein localization to cell tip22
affecting tea1alp4-1891Not specified
nuclear envelope protrusion present during mitosisalp41238 (1-238)Endogenous5
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in alp6 gamma tubulin complex Spc98/GCP3 subunit Alp6
FYPO affected by mutation in cut11 spindle pole body docking protein Cut11
FYPO affected by mutation in mzt1 mitotic spindle organizing protein Mzt1
GO localized by mcp6 horsetail movement protein Hrs1/Mcp6
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2523969..2527134
mRNA2523969..2527134
5' UTR2523969..2524060PMID:21511999
CDS2524061..2526415
3' UTR2526416..2527134PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04130 Pfam IPR007259 Gamma-tubulin complex component protein 74 693 4
PTHR19302:SF13 HMMPANTHER 60 782 1
PTHR19302 HMMPANTHER IPR007259 Gamma-tubulin complex component protein 60 782 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 115.00 Da
Charge -12.00
Isoelectric point 5.45
Molecular weight 90.16 kDa
Number of residues 784
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS6
S4, S6, S7
present during mitotic M phase, cellular response to thiabendazoleS39
present during mitotic M phaseS14
present during mitotic M phaseS7
present during mitotic M phaseS240
present during mitotic M phaseS4
present during mitotic M phaseS224
present during mitotic M phaseS35
present during mitotic M phaseS18
Annotation ExtensionEvidenceResidueReference
experimental evidence S4 PMID:24763107
present during mitotic M phase experimental evidence S4 PMID:21712547
experimental evidence S6 PMID:24763107
present during mitotic M phase experimental evidence S6 PMID:21712547
experimental evidence S7 PMID:24763107
present during mitotic M phase experimental evidence S7 PMID:21712547
present during mitotic M phase experimental evidence S14 PMID:21712547
present during mitotic M phase experimental evidence S18 PMID:21712547
present during mitotic M phase experimental evidence S35 PMID:21712547
present during mitotic M phase experimental evidence S39 PMID:21712547
present during cellular response to thiabendazole IDA S39 PMID:18257517
present during mitotic M phase experimental evidence S224 PMID:21712547
present during mitotic M phase experimental evidence S240 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
921during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
31.67during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.58during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byalp16gamma tubulin complex subunit Alp16 Affinity Capture-WesternPMID:21444751
affinity capturesalp16gamma tubulin complex subunit Alp16 Affinity Capture-MSPMID:15004232
affinity capturesalp16gamma tubulin complex subunit Alp16 Affinity Capture-WesternPMID:17021256
affinity captured byalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Affinity Capture-MSPMID:15004232
binds DNA-binding domain construct withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Two-hybridPMID:24006493
binds activation domain construct withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Two-hybridPMID:16855399
binds DNA-binding domain construct withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Two-hybridPMID:16855399
co-fractionates withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Co-fractionationPMID:23886939
affinity captured bygfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:21444751
affinity capturesgfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:15004232
affinity capturesgfh1gamma tubulin complex subunit Gfh1 Affinity Capture-MSPMID:15004232
affinity captured bygfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:15004232
affinity capturesgfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:17021256
affinity capturesgtb1gamma-tubulin Gtb1 Affinity Capture-WesternPMID:12134075
affinity capturesgtb1gamma-tubulin Gtb1 Affinity Capture-WesternPMID:11080156
affinity capturesgtb1gamma-tubulin Gtb1 Affinity Capture-MSPMID:15004232
binds activation domain construct withgtb1gamma-tubulin Gtb1 Two-hybridPMID:16855399
binds DNA-binding domain construct withhrs1mitochondrial and cytoplasmic histidine-tRNA ligase Hrs1 (predicted) Two-hybridPMID:22425159
affinity capturesmad2mitotic spindle checkpoint protein Mad2 Affinity Capture-WesternPMID:16855399
binds activation domain construct withmad2mitotic spindle checkpoint protein Mad2 Two-hybridPMID:16855399
affinity captured bymod21gamma tubulin complex subunit Mod21 Affinity Capture-WesternPMID:21444751
affinity capturesmod21gamma tubulin complex subunit Mod21 Affinity Capture-WesternPMID:17021256
affinity captured bymto1MT organizer Mto1 Affinity Capture-WesternPMID:15659644
affinity captured bymto1MT organizer Mto1 Affinity Capture-WesternPMID:15120067
affinity capturesmto1MT organizer Mto1 Affinity Capture-WesternPMID:15120067
affinity capturesmto1MT organizer Mto1 Affinity Capture-WesternPMID:15004232
affinity captured bymto1MT organizer Mto1 Affinity Capture-WesternPMID:15004232
affinity capturesmto1MT organizer Mto1 Affinity Capture-MSPMID:15004232
affinity captured bymzt1mitotic spindle organizing protein Mzt1 Affinity Capture-WesternPMID:23885124
affinity capturespcp1pericentrin Pcp1 Affinity Capture-WesternPMID:15004232
affinity captured bypkl1kinesin-like protein Pkl1 Affinity Capture-WesternPMID:23388459
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withalp16gamma tubulin complex subunit Alp16 Synthetic LethalityPMID:12134075
rescued by overexpression ofalp16gamma tubulin complex subunit Alp16 Dosage RescuePMID:12134075
overexpression rescuesalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Dosage RescuePMID:11080156
synthetically rescuescut7kinesin-like protein Cut7 Synthetic RescuePMID:18418055
synthetically rescued bygtb1gamma-tubulin Gtb1 Synthetic RescuePMID:15280226
phenotype enhanced bymad2mitotic spindle checkpoint protein Mad2 Phenotypic EnhancementPMID:11080156
synthetic growth defect withmad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:11080156
External References
Database Identifier Description
NBRP SPBC365.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC365.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC365.15 BioGRID Interaction Datasets
Expression Viewer SPBC365.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC365.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC365.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC365.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC365.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC365.15 Transcriptome Viewer (Bähler Lab)
GEO SPBC365.15 GEO profiles
PInt SPBC365.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC365.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC365.15 Fission yeast phenotypic data & analysis
Cyclebase SPBC365.15.1 Cell Cycle Data
SPD / RIKEN38/38C08Orfeome Localization Data
UniProtKB/SwissProtQ9Y705Spindle pole body component alp4
ModBaseQ9Y705Database of comparative protein structure models
STRINGQ9Y705Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596044gamma tubulin complex Spc97/GCP2 subunit Alp4
RefSeq mRNANM_001021954972h- gamma tubulin complex Spc97/GCP2 subunit Alp4 (alp4), mRNA
European Nucleotide ArchiveBAA77269.1ENA Protein Mapping
European Nucleotide ArchiveCAB44767.1ENA Protein Mapping
UniParcUPI00001258B7UniProt Archive

Literature for alp4

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015