alp4 (SPBC365.15)

Gene Standard Namealp4 ChromosomeII
Systematic IDSPBC365.15 Gene Start2523969
Synonyms Gene End2527134
Productgamma tubulin complex Spc97/GCP2 subunit Alp4 Gene Length3166
Feature Typeprotein coding CDS Start2524061
Name Description CDS End2526415
Characterisation Statuspublished CDS Length2355

Ensembl Gene Location
  1. ish1
  2. hba1
  3. uge1
  4. alp4
  5. SPNCRNA.1524
  6. SPNCRNA.399
  7. SPNCRNA.400
  8. SPBC365.16
  9. SPNCRNA.135
  10. ccr1
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000055long microtubulesno_name (unrecorded)unrecordedPECO:0000004PMID:11080156
FYPO:0000229cutno_name (unrecorded)unrecordedPMID:11080156
FYPO:0000276monopolar mitotic spindleno_name (unrecorded)unrecordedPECO:0000004PMID:11080156
FYPO:0002061inviable vegetative cell populationMicroscopyalp4delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0005515protein bindingIPImsd1PMID:17486116
IPImto1PMID:19001497
IPImto2PMID:19001497
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000910cytokinesisIMPPMID:11952833
GO:0007020microtubule nucleationICGO:0000930GO_REF:0000001
GO:0007094mitotic spindle assembly checkpointIMPPMID:11080156
GO:0010389regulation of G2/M transition of mitotic cell cycleIMPPMID:16611237
GO:0030473nuclear migration along microtubuleIMPPMID:16611237
GO:0031122cytoplasmic microtubule organizationIMPPMID:11080156
GO:0033566gamma-tubulin complex localizationIMPPMID:16611237
GO:0034631microtubule anchoring at spindle pole bodyIMPPMID:17486116
GO:0071963establishment or maintenance of cell polarity regulating cell shapeIMPPMID:9658169
happens_during interphase of mitotic cell cycleIMPPMID:16611237
GO:0090307spindle assembly involved in mitosisIMPPMID:11080156
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0000923equatorial microtubule organizing centerIDAPMID:11080156
GO:0000930gamma-tubulin complexTASPMID:11080156
GO:0005634nucleusIDAPMID:16823372
GO:0005816spindle pole bodyIDAPMID:11080156
GO:0005829cytosolIDAPMID:16823372
GO:0005874microtubuleIEAUniProtKB:KW-0493GO_REF:0000037
GO:0032153cell division siteIDAPMID:16823372
GO:0044732mitotic spindle pole bodyIDAPMID:16823372
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
125239692527134

References

Region Start End Reference
three_prime_UTR25264162527134PMID:21511999
three_prime_UTR25264162527134PMID:21511999
five_prime_UTR25239692524060PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF04130 IPR007259 Pfam Spc97/Spc98 74 693 4
PTHR19302:SF13 hmmpanther 3 782 1
PTHR19302 IPR007259 hmmpanther Spc97/Spc98 3 782 2

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:18257517

Protein Properties

Ave. residue weight 115.00 Da
Charge -12.00
Isoelectric point 5.45
Molecular weight 90.16 kDa
Number of residues 784
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencePECO:0000014,
PECO:0000005		population_wide31.67PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
RNA levelexperimental evidencePECO:0000014,
PECO:0000005		population_wide1.9PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.58PMID:23101633
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
alp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Dosage RescuePMID:11080156
gtb1gamma-tubulin Gtb1 Synthetic RescuePMID:15280226
mad2spindle checkpoint protein Mad2 Phenotypic EnhancementPMID:11080156
Synthetic Growth Defect
cut7kinesin-like protein Cut7 Synthetic RescuePMID:18418055
alp16gamma tubulin complex subunit Alp16 Dosage RescuePMID:12134075
Synthetic Lethality
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
alp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Co-localizationPMID:15004232
Affinity Capture-MS
Two-hybridPMID:16855399
mod21gamma tubulin complex subunit Mod21 Affinity Capture-WesternPMID:21444751
Affinity Capture-WesternPMID:17021256
gtb1gamma-tubulin Gtb1 Affinity Capture-MSPMID:15004232
Two-hybridPMID:16855399
Affinity Capture-WesternPMID:12134075
Affinity Capture-WesternPMID:11080156
kms1meiotic spindle pole body protein Kms1 Co-localizationPMID:23401002
mad2spindle checkpoint protein Mad2 Affinity Capture-WesternPMID:16855399
Two-hybrid
mto2MT organizer Mto2 Co-localizationPMID:15837798
pcp1pericentrin Pcp1 Affinity Capture-WesternPMID:15004232
alp16gamma tubulin complex subunit Alp16 Co-localizationPMID:15004232
Affinity Capture-MS
Affinity Capture-WesternPMID:21444751
Affinity Capture-WesternPMID:17021256
gfh1gamma tubulin complex subunit Gfh1 Co-localizationPMID:15004232
Affinity Capture-Western
Affinity Capture-MS
Affinity Capture-WesternPMID:21444751
Affinity Capture-WesternPMID:17021256
mto1MT organizer Mto1 Affinity Capture-WesternPMID:15004232
Co-localization
Affinity Capture-MS
Co-localizationPMID:23401002
Affinity Capture-WesternPMID:15120067
Affinity Capture-WesternPMID:15659644
hrs1mitochondrial and cytoplasmic histidine-tRNA ligase Hrs1 (predicted) Two-hybridPMID:22425159
sad1spindle pole body protein Sad1 Co-localizationPMID:23401002
pkl1kinesin-like protein Pkl1 Affinity Capture-WesternPMID:23388459
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External References
Database Identifier Description
NBRP SPBC365.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC365.15 Retrieval of eukaryotic orthologs
BioGrid SPBC365.15 BioGRID Interaction Datasets
Bähler Lab SPBC365.15 Cell Cycle Expression Profile
Bähler Lab SPBC365.15 Meiosis/Sporulation Expression Profies
Bähler Lab SPBC365.15 Pheromone response/mating expression profiles
Bähler Lab SPBC365.15 Environmental stress expression profiles
Bähler Lab SPBC365.15 Bähler Lab Transcriptome Viewer
Cyclebase SPBC365.15 Cell Cycle Data
PInt SPBC365.15 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPBC365.15 Entrez Gene
EntrezGene2540392gamma tubulin complex Spc97/GCP2 subunit Alp4
Uniprot_genenameALP4
WikiGene2540392gamma tubulin complex Spc97/GCP2 subunit Alp4
UniProtKB/SwissProtQ9Y705Spindle pole body component alp4
ModBaseQ9Y705Database of comparative protein structure models
Pfam Protein DomainsQ9Y705Pfam Domain Arrangement
RefSeq PeptideNP_596044gamma tubulin complex Spc97/GCP2 subunit Alp4
RefSeq mRNANM_001021954972h- gamma tubulin complex Spc97/GCP2 subunit Alp4 (alp4), mRNA
European Nucleotide ArchiveBAA77269ENA Protein Mapping
European Nucleotide ArchiveCAB44767ENA Protein Mapping
SPD / RIKEN38/38C08Orfeome Localization Data
UniParcUPI00001258B7UniProt Archive

Literature for alp4
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013