nup120 (SPBC3B9.16c)


Gene Standard Namenup120 Characterisation Statuspublished
Systematic IDSPBC3B9.16c Feature Typeprotein coding
Synonyms Name Description
Productnucleoporin Nup120 Product Size1136aa, 129.78 kDa
Genomic Location Chromosome II, 4018932-4015226 (3707nt); CDS:4018767-4015357 (3411nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding851
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitotic sister chromatid segregation200
Annotation ExtensionEvidenceWith/FromReference
poly(A)+ mRNA export from nucleus17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear envelope136
Annotation ExtensionEvidenceWith/FromReference
nuclear periphery41
Annotation ExtensionEvidenceWith/FromReference
nuclear pore53
Annotation ExtensionEvidenceWith/FromReference
nuclear pore outer ring7
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at low temperaturenup120ΔNull11
decreased cell population growth on glucose carbon sourcenup120ΔNull154
inviable vegetative cell populationnup120ΔNull1428
unnamed (unrecorded)
viable spore populationnup120ΔNull27

Cell Phenotype

Term NameAlleleExpressionCount
abolished protein binding78
affecting nup37 and nup120nup120PD (D417A, E420A)Endogenous
decreased protein binding52
affecting nup37 and nup120unnamed (479-488)Endogenous
dispersed actin cortical patch localizationnup120ΔNull26
increased RNA level during vegetative growth173
affecting ssm4nup120ΔNull
affecting spo5nup120ΔNull
affecting rec8nup120ΔNull
affecting mei4nup120ΔNull
inviable after spore germination, multiple cell divisions, abnormal morphologynup120ΔNull192
inviable after spore germination, multiple cell divisions, normal septum morphologynup120ΔNull4
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in nup120 nucleoporin Nup120 PMID:22955883
FYPO affected by mutation in nup37 WD repeat protein, nucleoporin Nup37 PMID:22955883
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
140189324015226

UTRs

Region Coordinates Reference
five_prime_UTR4018932..4018768PMID:21511999
three_prime_UTR4015356..4015226PMID:21511999
exon4018767..4015357
mRNA4018932..4015226
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11715 Pfam IPR021717 Nucleoporin Nup120/160 24 551 1
PTHR21286 HMMPANTHER 1 1114 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.25 Da
Charge -41.00
Isoelectric point 4.63
Molecular weight 129.78 kDa
Number of residues 1136
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8230during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
8332during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7190during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
10501during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
8210during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1376.79during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only619
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withely5nuclear pore protein Ely5 Reconstituted ComplexPMID:22955883
affinity capturesely5nuclear pore protein Ely5 Affinity Capture-WesternPMID:22955883
nup37WD repeat protein, nucleoporin Nup37 Co-crystal or NMR structurePMID:22955883
forms complex withnup37WD repeat protein, nucleoporin Nup37 Reconstituted ComplexPMID:22955883
forms complex withnup37WD repeat protein, nucleoporin Nup37 Reconstituted ComplexPMID:22955883
forms complex withnup37WD repeat protein, nucleoporin Nup37 Reconstituted ComplexPMID:23019579
nup37WD repeat protein, nucleoporin Nup37 Co-crystal or NMR structurePMID:23019579
affinity captured bynup85nucleoporin Nup85 Affinity Capture-WesternPMID:15226438
affinity captured byseh1Seh1-associated complex subunity Seh1 Affinity Capture-WesternPMID:15226438
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withred5human ZC3H3 homolog Synthetic Growth DefectPMID:23658229
External References
Database Identifier Description
NBRP SPBC3B9.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC3B9.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC3B9.16c BioGRID Interaction Datasets
Expression Viewer SPBC3B9.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC3B9.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC3B9.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC3B9.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC3B9.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC3B9.16c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC3B9.16c Cell Cycle Data
GEO SPBC3B9.16c GEO profiles
PInt SPBC3B9.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC3B9.16c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC3B9.16c Fission yeast phenotypic data & analysis
SPD / RIKEN30/30E02Orfeome Localization Data
UniProtKB/SwissProtO43044Nucleoporin nup120
ModBaseO43044Database of comparative protein structure models
STRINGO43044Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596674nucleoporin Nup120
RefSeq mRNANM_001022596972h- nucleoporin Nup120 (nup120), mRNA
ePDB4FHMThe European PDB
PDB4FHMPDB
PDBsum4FHMPDBsum
ePDB4FHNThe European PDB
PDB4FHNPDB
PDBsum4FHNPDBsum
ePDB4GQ2The European PDB
PDB4GQ2PDB
PDBsum4GQ2PDBsum
European Nucleotide ArchiveCAA17796.1ENA Protein Mapping
UniParcUPI000006B172UniProt Archive

Literature for nup120

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015