nup120 (SPBC3B9.16c)


Gene Standard Namenup120 Characterisation Statuspublished
Systematic IDSPBC3B9.16c Feature Typeprotein coding
Synonyms Name Description
Productnucleoporin Nup120 Product Size1136aa, 129.78 kDa
Genomic Location Chromosome II, 4018932-4015226 (3707nt); CDS:4018767-4015357 (3411nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPInup85PMID:15226438865
IPIely5PMID:22955883
IPInup37PMID:22955883
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000070mitotic sister chromatid segregationIMPPMID:15226438131
GO:0031081nuclear pore distributionIMPPMID:152264382
GO:0016973poly(A)+ mRNA export from nucleusIMPPMID:1522643817
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016020membraneIMPPMID:152264381450
GO:0005635nuclear envelopeIDAPMID:16823372137
GO:0031965nuclear membraneIDAPMID:1511643251
GO:0005643nuclear poreIDAPMID:1522643854
during mitotic cell cycleIDAPMID:20970342
during meiotic cell cycleIDAPMID:20970342
GO:0031080nuclear pore outer ringIDAPMID:152264387
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002175decreased cell population growth at low temperatureCell growth assaynup120ΔNullPECO:0000102, PECO:0000137PMID:236582295
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaynup120ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:23658229119
FYPO:0002061inviable vegetative cell populationCell growth assaynup120ΔNullPECO:0000004, PECO:0000102, PECO:0000137PMID:236582291337
Microscopynup120ΔNullPMID:20473289
Microscopynot recorded (unrecorded)PMID:15226438

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000705abolished protein binding56
affecting nup37 and nup120Othernup120PD (D417A, E420A)EndogenousPMID:22955883
FYPO:0001645decreased protein binding35
affecting nup37 and nup120Othernot recorded (479-488)EndogenousPMID:22955883
FYPO:0002021dispersed actin cortical patch localizationMicroscopynup120ΔNullPECO:0000005, PECO:0000137PMID:2369780621
FYPO:0000825increased RNA level during vegetative growth122
affecting mei4Transcript expression level evidencenup120ΔNullPMID:23658229
affecting rec8Transcript expression level evidencenup120ΔNullPMID:23658229
affecting ssm4Transcript expression level evidencenup120ΔNullPMID:23658229
affecting spo5Transcript expression level evidencenup120ΔNullPMID:23658229
FYPO:0002148inviable after spore germination, multiple cell divisions, abnormal morphology187
expressivity FYPO_EXT:0000003Microscopynup120ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in nup120 nucleoporin Nup120 PMID:22955883
FYPO affected by mutation in nup37 WD repeat protein, nucleoporin Nup37 PMID:22955883
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
140189324015226
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11715 Pfam IPR021717 Nucleoporin Nup120/160 24 551 1
PTHR21286 HMMPANTHER 8 1097 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.25 Da
Charge -41.00
Isoelectric point 4.63
Molecular weight 129.78 kDa
Number of residues 1136
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8230during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
8332during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7190during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
10501during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
8210during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1376.79during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4599
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
red5human ZC3H3 homolog Synthetic Growth DefectPMID:23658229
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
nup85nucleoporin Nup85 Affinity Capture-WesternPMID:15226438
ely5nuclear pore protein Ely5 Affinity Capture-WesternPMID:22955883
Reconstituted Complex
nup37WD repeat protein, nucleoporin Nup37 Co-crystal or NMR structurePMID:22955883
Reconstituted Complex
Reconstituted ComplexPMID:23019579
Co-crystal or NMR structure
seh1Seh1-associated complex subunity Seh1 Affinity Capture-WesternPMID:15226438
External References
Database Identifier Description
NBRP SPBC3B9.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC3B9.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC3B9.16c BioGRID Interaction Datasets
Expression Viewer SPBC3B9.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC3B9.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC3B9.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC3B9.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC3B9.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC3B9.16c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC3B9.16c Cell Cycle Data
GEO SPBC3B9.16c GEO profiles
PInt SPBC3B9.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC3B9.16c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN30/30E02Orfeome Localization Data
UniProtKB/SwissProtO43044Nucleoporin nup120
ModBaseO43044Database of comparative protein structure models
STRINGO43044Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596674nucleoporin Nup120
RefSeq mRNANM_001022596972h- nucleoporin Nup120 (nup120), mRNA
ePDB4FHMThe European PDB
PDB4FHMPDB
PDBsum4FHMPDBsum
ePDB4FHNThe European PDB
PDB4FHNPDB
PDBsum4FHNPDBsum
ePDB4GQ2The European PDB
PDB4GQ2PDB
PDBsum4GQ2PDBsum
European Nucleotide ArchiveCAA17796.1ENA Protein Mapping
UniParcUPI000006B172UniProt Archive

Literature for nup120

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014