nup120 (SPBC3B9.16c)


Gene Standard Namenup120 Characterisation Statuspublished
Systematic IDSPBC3B9.16c Feature Typeprotein coding
Synonyms Name Description
Productnucleoporin Nup120 Product Size1136aa, 129.78 kDa
Genomic Location Chromosome II, 4018932-4015226 (3707nt); CDS:4018767-4015357 (3411nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding868
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
poly(A)+ mRNA export from nucleus17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear envelope129
Annotation ExtensionEvidenceWith/FromReference
nuclear periphery45
Annotation ExtensionEvidenceWith/FromReference
nuclear pore51
nuclear pore outer ring7
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
abolished cell population growth at high temperaturenup120Δ34
decreased cell population growth at low temperaturenup120Δ87
decreased cell population growth on glucose carbon sourcenup120Δ319
inviable vegetative cell population1451
nup120Δ
sensitive to hydroxyureanup120Δ580
sensitive to thiabendazolenup120Δ326
viable spore populationnup120Δ46

Cell Phenotype

Term NameGenotypesCount
abnormal sporulation resulting in formation of ascus with fewer than four sporesnup120Δ76
abolished protein binding126
affecting nup37 and nup120nup120PD (D417A, E420A)
decreased protein binding89
affecting nup37 and nup120 (479-488)
dispersed actin cortical patch localizationnup120Δ32
increased RNA level during vegetative growth256
affecting ssm4nup120Δ
affecting spo5nup120Δ
affecting rec8nup120Δ
affecting mei4nup120Δ
inviable after spore germination, multiple cell divisions, abnormal morphologynup120Δ205
inviable after spore germination, multiple cell divisions, normal septum morphologynup120Δ4
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in nup120 nucleoporin Nup120
FYPO affected by mutation in nup37 WD repeat protein, nucleoporin Nup37
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4018932..4015226
mRNA4018932..4015226
5' UTR4018932..4018768PMID:21511999
CDS4018767..4015357
3' UTR4015356..4015226PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11715 Pfam 24 551 2
PTHR21286 HMMPANTHER 1 1102 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.25 Da
Charge -41.00
Codon Adaptation Index 0.41
Isoelectric point 4.63
Molecular weight 129.78 kDa
Number of residues 1136
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for nup120 (SPBC3B9.16c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8230during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8332during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
7190during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
10501during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1376.79during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
8210during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only618
conserved in fungi4606
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC3B9.16c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withely5nuclear pore protein Ely5 Reconstituted ComplexPMID:22955883
forms complex withnup37WD repeat protein, nucleoporin Nup37 Reconstituted ComplexPMID:23019579
affinity capturesely5nuclear pore protein Ely5 Affinity Capture-WesternPMID:22955883
affinity captured bynup85nucleoporin Nup85 Affinity Capture-WesternPMID:15226438
affinity captured byseh1Seh1-associated complex subunit Seh1 Affinity Capture-WesternPMID:15226438
binds DNA-binding domain construct witheso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Two-hybridPMID:26771498
binds DNA-binding domain construct withnup37WD repeat protein, nucleoporin Nup37 Two-hybridPMID:26771498
co-locates (via crystalisation or NMR) withnup37WD repeat protein, nucleoporin Nup37 Co-crystal or NMR structurePMID:22955883
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC3B9.16c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withred5human ZC3H3 homolog Synthetic Growth DefectPMID:23658229
External References
Database Identifier Description
NBRP SPBC3B9.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC3B9.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC3B9.16c BioGRID Interaction Datasets
Expression Viewer SPBC3B9.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC3B9.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC3B9.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC3B9.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC3B9.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC3B9.16c Transcriptome Viewer (Bähler Lab)
GEO SPBC3B9.16c GEO profiles
PInt SPBC3B9.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC3B9.16c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC3B9.16c Fission yeast phenotypic data & analysis
Cyclebase SPBC3B9.16c.1 Cell Cycle Data
SPD / RIKEN30/30E02Orfeome Localization Data
UniProtKB/SwissProtO43044Nucleoporin nup120
ModBaseO43044Database of comparative protein structure models
STRINGO43044Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596674nucleoporin Nup120
RefSeq mRNANM_001022596972h- nucleoporin Nup120 (nup120), mRNA

Literature for nup120

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016