mge1 (SPBC3B9.19)


Gene Standard Namemge1 Characterisation Statusbiological role inferred
Systematic IDSPBC3B9.19 Feature Typeprotein coding
Synonyms Name Description
Productmitochondrial GrpE domain chaperone protein (predicted) Product Size223aa, 25.33 kDa
Genomic Location Chromosome II, 4022474-4023772 (1299nt); CDS:4022547-4023218 (672nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
adenyl-nucleotide exchange factor activity5
Annotation ExtensionEvidenceWith/FromReference
chaperone binding8
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding43
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
protein folding102
Annotation ExtensionEvidenceWith/FromReference
protein import into mitochondrial matrix20
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
presequence translocase-associated import motor6
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationmge1Δ1444

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination, without cell divisionmge1Δ492
inviable swollen sporemge1Δ3
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4022474..4023772
mRNA4022474..4023772
5' UTR4022474..4022546PMID:21511999
CDS4022547..4023218
3' UTR4023219..4023772PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01025 Pfam IPR000740 GrpE nucleotide exchange factor 52 220 1
PS01071 Prosite Patterns IPR000740 GrpE nucleotide exchange factor 175 218 1
PTHR21237 HMMPANTHER IPR000740 GrpE nucleotide exchange factor 6 223 1
PTHR21237:SF23 HMMPANTHER 6 223 1
1dkgA02 Gene3D IPR009012 GrpE nucleotide exchange factor, head 164 222 1
1dkgA01 Gene3D IPR013805 GrpE nucleotide exchange factor, coiled-coil 56 162 1
0053405 SuperFamily 59 164 1
0036470 SuperFamily IPR009012 GrpE nucleotide exchange factor, head 166 222 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 46 101 1048
MF_01151 hamap IPR000740 GrpE nucleotide exchange factor 23 220 1
PR00773 PRINTS IPR000740 GrpE nucleotide exchange factor 82 98 1
PR00773 PRINTS IPR000740 GrpE nucleotide exchange factor 110 125 1
PR00773 PRINTS IPR000740 GrpE nucleotide exchange factor 199 218 1
PR00773 PRINTS IPR000740 GrpE nucleotide exchange factor 172 187 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.61 Da
Charge 2.00
Isoelectric point 8.39
Molecular weight 25.33 kDa
Number of residues 223
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
40246during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
40400during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
31161during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
37536during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
31942during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
11366.36during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
12954.12during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3421
conserved in vertebrates3396
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPBC3B9.19 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
External References
Database Identifier Description
NBRP SPBC3B9.19 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC3B9.19 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC3B9.19 BioGRID Interaction Datasets
Expression Viewer SPBC3B9.19 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC3B9.19 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC3B9.19 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC3B9.19 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC3B9.19 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC3B9.19 Transcriptome Viewer (Bähler Lab)
GEO SPBC3B9.19 GEO profiles
PInt SPBC3B9.19 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC3B9.19 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC3B9.19 Fission yeast phenotypic data & analysis
Cyclebase SPBC3B9.19.1 Cell Cycle Data
SPD / RIKEN07/07G03Orfeome Localization Data
UniProtKB/SwissProtO43047GrpE protein homolog, mitochondrial
ModBaseO43047Database of comparative protein structure models
STRINGO43047Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596677mitochondrial GrpE domain chaperone protein (predicted)
RefSeq mRNANM_001022599972h- mitochondrial GrpE domain chaperone protein (predicted) (mge1), mRNA
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveCAA17799ENA Protein Mapping
European Nucleotide ArchiveCAA17799.1ENA Protein Mapping
UniParcUPI000012BB38UniProt Archive

Literature for mge1

Search: Europe PMC or PubMed

Release Version: PomBase:29_56 - 12 Nov 2015