hsp16 (SPBC3E7.02c)

Gene Standard Namehsp16 Characterisation Statuspublished
Systematic IDSPBC3E7.02c Feature Typeprotein coding
Synonyms Name Description
Productheat shock protein Hsp16 Product Size143aa, 15.97 kDa
Genomic Location Chromosome II, 2660992-2660139 (854nt); CDS:2660921-2660490 (432nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
identical protein binding7
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding42
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to starvation115
Annotation ExtensionEvidenceWith/FromReference
cellular response to unfolded protein15
Annotation ExtensionEvidenceWith/FromReference
chaperone-mediated protein folding10
Annotation ExtensionEvidenceWith/FromReference
mRNA export from nucleus in response to heat stress3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000088sensitive to hydroxyureaCell growth assayhsp16ΔNullPECO:0000137, PECO:0000005PMID:23173672514
FYPO:0002060viable vegetative cell populationMicroscopyhsp16ΔNullPMID:204732893759
Microscopyhsp16ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyhsp16ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
FYPO affected by mutation in dcr1 dicer PMID:15947136
FYPO affected by mutation in rpb2 RNA polymerase II complex subunit Rpb2 PMID:15947136
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00011 Pfam IPR002068 Alpha crystallin/Hsp20 domain 40 142 2
PS01031 Prosite Profiles IPR002068 Alpha crystallin/Hsp20 domain 40 130 2
PTHR11527 HMMPANTHER 6 142 2 Gene3D 4 143 6
SSF49764 SuperFamily IPR008978 HSP20-like chaperone 6 138 6

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.66 Da
Charge -1.00
Isoelectric point 5.80
Molecular weight 15.97 kDa
Number of residues 143

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
present during cellular response to thiabendazole
MOD:00696phosphorylated residueNASPMID:182575171922
Gene Expression

Qualitative Gene Expression

RNA levelincreased during GO:0034605expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0034599expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
428030during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
373751during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
377672during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
622767during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
8800.96during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
438602during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
14144.36during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
11during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Disease Association
neurological disorders PMID:2329357898
Charcot-Marie-Tooth disease PMID:232935789
Subunit Composition
dimeric PMID:232734291
hexadecameric PMID:232734291
Miscellaneous Functional Group
Species Distribution
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
pmt3SUMO Two-hybridPMID:11452028
tef3translation elongation factor eEF3 Two-hybridPMID:11452028
rhp18Rad18 homolog ubiquitin protein ligase E3, Rhp18 Two-hybridPMID:11452028
tup12transcriptional corepressor Tup12 Two-hybridPMID:11452028
rfp1SUMO-targeted ubiquitin-protein ligase subunit Rfp1 Two-hybridPMID:11452028
mug190C2 domain protein Two-hybridPMID:11452028
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
hsp16heat shock protein Hsp16 Two-hybridPMID:11452028
Co-crystal or NMR structurePMID:23273429
pfl5cell surface glycoprotein (predicted) Two-hybridPMID:11452028
pek1MAP kinase kinase Pek1 Two-hybridPMID:23695164
eft201translation elongation factor 2 (EF-2) Eft2,A Two-hybridPMID:11452028
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Positive GeneticPMID:22681890
dad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
cdc18MCM loader Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC3E7.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC3E7.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC3E7.02c BioGRID Interaction Datasets
Expression Viewer SPBC3E7.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC3E7.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC3E7.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC3E7.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC3E7.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC3E7.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC3E7.02c Cell Cycle Data
GEO SPBC3E7.02c GEO profiles
PInt SPBC3E7.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC3E7.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC3E7.02c Fission yeast phenotypic data & analysis
SPD / RIKEN31/31D08Orfeome Localization Data
UniProtKB/SwissProtO14368Heat shock protein 16
ModBaseO14368Database of comparative protein structure models
STRINGO14368Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596091heat shock protein Hsp16
RefSeq mRNANM_001022007972h- heat shock protein Hsp16 (hsp16), mRNA
European Nucleotide ArchiveAJ003817ENA EMBL mapping
ePDB3W1ZThe European PDB
European Nucleotide ArchiveBAA31521.1ENA Protein Mapping
European Nucleotide ArchiveCAA19006.1ENA Protein Mapping
UniParcUPI000012CBFDUniProt Archive

Literature for hsp16

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014