hsp16 (SPBC3E7.02c)


Gene Standard Namehsp16 Characterisation Statuspublished
Systematic IDSPBC3E7.02c Feature Typeprotein coding
Synonyms Name Description
Productheat shock protein Hsp16 Product Size143aa, 15.97 kDa
Genomic Location Chromosome II, 2660992-2660139 (854nt); CDS:2660921-2660490 (432nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
identical protein binding3
Annotation ExtensionEvidenceWith/FromReference
protein binding872
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding42
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to misfolded protein11
Annotation ExtensionEvidenceWith/FromReference
cellular response to unfolded protein14
Annotation ExtensionEvidenceWith/FromReference
chaperone-mediated protein folding10
Annotation ExtensionEvidenceWith/FromReference
mRNA export from nucleus in response to heat stress2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4212
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2691
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
loss of viability upon nitrogen starvationhsp16Δ53
sensitive to hydroxyureahsp16Δ580
viable vegetative cell populationhsp16Δ3844

Cell Phenotype

Term NameGenotypesCount
decreased protein aggregate fusionhsp16Δ1
viable vegetative cell with normal cell morphologyhsp16Δ3100
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in chp1 chromodomain protein Chp1
FYPO affected by mutation in clr4 histone H3 lysine methyltransferase Clr4
FYPO affected by mutation in dcr1 dicer
FYPO affected by mutation in hip1 hira protein, histone chaperone Hip1
FYPO affected by mutation in hip3 HIRA interacting protein Hip3
FYPO affected by mutation in rik1 silencing protein Rik1
FYPO affected by mutation in rpb2 RNA polymerase II complex subunit Rpb2
FYPO affected by mutation in slm9 hira protein Slm9
FYPO affected by mutation in swi6 HP1 family chromodomain protein Swi6
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2660992..2660139
mRNA2660992..2660139
5' UTR2660992..2660922SPHSP16
CDS2660921..2660490
3' UTR2660489..2660139SPHSP16
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00011 Pfam IPR002068 Alpha crystallin/Hsp20 domain 40 142 2
PS01031 Prosite Profiles IPR002068 Alpha crystallin/Hsp20 domain 40 130 2
PTHR11527 HMMPANTHER IPR031107 Small heat shock protein HSP20 5 142 2
PTHR11527:SF42 HMMPANTHER 5 142 1
2.60.40.790 Gene3D IPR008978 HSP20-like chaperone 4 143 6
SSF49764 SuperFamily IPR008978 HSP20-like chaperone 6 138 6

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.66 Da
Charge -1.00
Codon Adaptation Index 0.61
Isoelectric point 5.80
Molecular weight 15.97 kDa
Number of residues 143
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during cellular response to thiabendazoleS99
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S99 PMID:18257517
phosphorylated residue 1932
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0034605expression microarray evidencePMID:12529438
increased during GO:0034599expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for hsp16 (SPBC3E7.02c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
428030during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
373751during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
377672during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
622767during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8800.96during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
438602during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14144.36during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
11during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
neurological disorders PMID:23293578100
Charcot-Marie-Tooth disease PMID:232935789
Subunit Composition
DescriptionQualifierReferenceCount
dimeric PMID:232734291
hexadecameric PMID:232734291
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
chaperone12
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in eukaryotes4516
conserved in bacteria1002
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC3E7.02c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byimp1importin alpha Affinity Capture-MSPMID:25963819
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
binds activation domain construct withmug190C2 domain protein involved in ER membrane tethering Two-hybridPMID:11452028
binds activation domain construct withtup12transcriptional corepressor Tup12 Two-hybridPMID:11452028
binds activation domain construct withhsp16heat shock protein Hsp16 Two-hybridPMID:11452028
binds activation domain construct withpek1MAP kinase kinase Pek1 Two-hybridPMID:23695164
binds activation domain construct witheft201translation elongation factor 2 (EF-2) Eft2,A Two-hybridPMID:11452028
binds activation domain construct withrhp18Rad18 homolog ubiquitin protein ligase E3, Rhp18 Two-hybridPMID:11452028
binds activation domain construct withpfl5cell surface glycoprotein (predicted) Two-hybridPMID:11452028
binds activation domain construct withtef3translation elongation factor eEF3 Two-hybridPMID:11452028
binds activation domain construct withrfp1SUMO-targeted ubiquitin-protein ligase subunit Rfp1 Two-hybridPMID:11452028
binds activation domain construct withpmt3SUMO Two-hybridPMID:11452028
binds DNA-binding domain construct withhgh1conserved eukaryotic protein Hgh1 Two-hybridPMID:26771498
co-locates (via crystalisation or NMR) withhsp16heat shock protein Hsp16 Co-crystal or NMR structurePMID:23273429
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC3E7.02c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withcdc18MCM loader Negative GeneticPMID:22681890
negative genetic interaction withdad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Positive GeneticPMID:22681890
positive genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC3E7.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC3E7.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC3E7.02c BioGRID Interaction Datasets
Expression Viewer SPBC3E7.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC3E7.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC3E7.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC3E7.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC3E7.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC3E7.02c Transcriptome Viewer (Bähler Lab)
GEO SPBC3E7.02c GEO profiles
PInt SPBC3E7.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC3E7.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC3E7.02c Fission yeast phenotypic data & analysis
Cyclebase SPBC3E7.02c.1 Cell Cycle Data
SPD / RIKEN31/31D08Orfeome Localization Data
UniProtKB/SwissProtO14368Heat shock protein 16
ModBaseO14368Database of comparative protein structure models
STRINGO14368Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596091heat shock protein Hsp16
RefSeq mRNANM_001022007972h- heat shock protein Hsp16 (hsp16), mRNA
European Nucleotide ArchiveAJ003817ENA EMBL mapping
ePDB3W1ZThe European PDB
PDB3W1ZPDB
PDBsum3W1ZPDBsum
European Nucleotide ArchiveBAA31521.1ENA Protein Mapping
European Nucleotide ArchiveCAA19006.1ENA Protein Mapping
UniParcUPI000012CBFDUniProt Archive

Literature for hsp16

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016