hsp16 (SPBC3E7.02c)

Gene Standard Namehsp16 Characterisation Statuspublished
Systematic IDSPBC3E7.02c Feature Typeprotein coding
Synonyms Name Description
Productheat shock protein Hsp16 Product Size143aa, 15.97 kDa
Genomic Location Chromosome II, 2660992-2660139 (854nt); CDS:2660921-2660490 (432nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0042802identical protein bindingIPIhsp16PMID:2327342916
GO:0005515protein bindingIPIhsp16PMID:11452028861
GO:0051082unfolded protein bindingIDAPMID:1605543742
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0009267cellular response to starvationIEPPMID:11452028113
GO:0034620cellular response to unfolded proteinIDAPMID:1605543715
GO:0061077chaperone-mediated protein foldingNASGO_REF:000000111
GO:0031990mRNA export from nucleus in response to heat stressIMPPMID:158409443
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000088sensitive to hydroxyureaCell growth assayhsp16ΔNullPECO:0000137, PECO:0000005PMID:23173672511
FYPO:0002060viable vegetative cell populationMicroscopyhsp16ΔNullPMID:204732893730
Microscopyhsp16ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyhsp16ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
FYPO affected by mutation in dcr1 dicer PMID:15947136
FYPO affected by mutation in rpb2 RNA polymerase II complex subunit Rpb2 PMID:15947136
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00011 Pfam IPR002068 Alpha crystallin/Hsp20 domain 40 142 2
PS01031 Prosite Profiles IPR002068 Alpha crystallin/Hsp20 domain 40 130 2
PTHR11527 HMMPANTHER 5 143 2 Gene3D IPR008978 HSP20-like chaperone 4 143 6
SSF49764 SuperFamily IPR008978 HSP20-like chaperone 6 138 6

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.66 Da
Charge -1.00
Isoelectric point 5.80
Molecular weight 15.97 kDa
Number of residues 143

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
present during cellular response to thiabendazole
MOD:00696phosphorylated residueNASPMID:182575171921
Gene Expression

Qualitative Gene Expression

RNA levelincreased during GO:0034599expression microarray evidencePMID:12529438
increased during GO:0034605expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
428030during GO:0000080PECO:0000005,
mass spectrometry evidencePMID:24763107
373751during GO:0000084PECO:0000005,
mass spectrometry evidencePMID:24763107
377672during GO:0000085PECO:0000005,
mass spectrometry evidencePMID:24763107
622767during GO:0000087PECO:0000005,
mass spectrometry evidencePMID:24763107
8800.96during GO:0072690PECO:0000014,
experimental evidencePMID:23101633
438602during GO:0072690PECO:0000005,
mass spectrometry evidencePMID:24763107
14144.36during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
11during GO:0072690PECO:0000014,
experimental evidencePMID:23101633
7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
experimental evidencePMID:23101633
Subunit Composition
dimeric PMID:232734291
hexadecameric PMID:232734291
Miscellaneous Functional Group
Species Distribution
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Positive GeneticPMID:22681890
dad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
cdc18MCM loader Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
pmt3SUMO Two-hybridPMID:11452028
tef3translation elongation factor eEF3 Two-hybridPMID:11452028
rhp18Rad18 homolog Rhp18 Two-hybridPMID:11452028
tup12transcriptional corepressor Tup12 Two-hybridPMID:11452028
rfp1SUMO-targeted ubiquitin-protein ligase subunit Rfp1 Two-hybridPMID:11452028
mug190C2 domain protein Two-hybridPMID:11452028
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
hsp16heat shock protein Hsp16 Two-hybridPMID:11452028
Co-crystal or NMR structurePMID:23273429
pfl5cell surface glycoprotein (predicted) Two-hybridPMID:11452028
pek1MAP kinase kinase Pek1 Two-hybridPMID:23695164
eft201translation elongation factor 2 (EF-2) Eft2,A Two-hybridPMID:11452028
External References
Database Identifier Description
NBRP SPBC3E7.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC3E7.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC3E7.02c BioGRID Interaction Datasets
Expression Viewer SPBC3E7.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC3E7.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC3E7.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC3E7.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC3E7.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC3E7.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC3E7.02c Cell Cycle Data
GEO SPBC3E7.02c GEO profiles
PInt SPBC3E7.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC3E7.02c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN31/31D08Orfeome Localization Data
EntrezGene2540977heat shock protein Hsp16
WikiGene2540977heat shock protein Hsp16
UniProtKB/SwissProtO14368Heat shock protein 16
ModBaseO14368Database of comparative protein structure models
STRINGO14368Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596091heat shock protein Hsp16
RefSeq mRNANM_001022007972h- heat shock protein Hsp16 (hsp16), mRNA
European Nucleotide ArchiveAJ003817ENA EMBL mapping
ePDB3W1ZThe European PDB
European Nucleotide ArchiveBAA31521ENA Protein Mapping
European Nucleotide ArchiveBAA31521.1ENA Protein Mapping
European Nucleotide ArchiveCAA06031ENA Protein Mapping
European Nucleotide ArchiveCAA19006ENA Protein Mapping
European Nucleotide ArchiveCAA19006.1ENA Protein Mapping
UniParcUPI000012CBFDUniProt Archive

Literature for hsp16

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014