trr1 (SPBC3F6.03)


Gene Standard Nametrr1 Characterisation Statuspublished
Systematic IDSPBC3F6.03 Feature Typeprotein coding
Synonymscaf4, trxR Name Description
Productthioredoxin reductase Trr1 Product Size322aa, 34.62 kDa
Genomic Location Chromosome II, 4189144-4191448 (2305nt); CDS:4189967-4191234 (1268nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding887
Annotation ExtensionEvidenceWith/FromReference
thioredoxin-disulfide reductase activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

detoxification


Term NameCount
cellular oxidant detoxification10
Annotation ExtensionEvidenceWith/FromReference
removal of superoxide radicals10
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
mitochondrion752
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth on glucose carbon sourcetrr1Δ380
decreased sporulation frequencycaf4-83103
normal anaerobic cell population growthtrr1Δ8
resistance to aluminiumtrr1+3
resistance to brefeldin Acaf4-8319
resistance to caffeinecaf4-8326
trr1Δ
resistance to hydrogen peroxidecaf4-8375
resistance to ionizing radiation during vegetative growthcaf4-832
resistance to mercurytrr1+7
sensitive to high pHtrr1Δ6
caf4-83
sensitive to hydrogen peroxidetrr1Δ155
sensitive to UV during vegetative growthcaf4-83161
trr1Δ
slow vegetative cell population growthcaf4-83367
viable vegetative cell populationtrr1Δ3850

Cell Phenotype

Term NameGenotypesCount
decreased protein level during cellular response to menadione4
affecting trr1pYUTR40 (-186--499)
decreased protein level during vegetative growth153
affecting trr1pYUTR30 (-499--891)
increased protein binding18
affecting prr1 and pap1trr1Δ
increased protein localization to nucleus during vegetative growth23
affects localization of pap1trr1Δ
increased protein oxidation during vegetative growth4
affecting pap1trr1Δ
affecting txl1trr1Δ
increased RNA level during vegetative growth342
affecting obr1trr1Δ
affecting srx1trr1Δ
affecting caf5trr1Δ
affecting SPCC663.08ctrr1Δ
affecting ctt1trr1Δ
increased thioredoxin-disulfide reductase activitytrr1+1
viable vegetative cell with normal cell morphologytrr1Δ3102

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
resistance to caffeinetrr1Δ, bfr1Δ
sensitive to caffeine during vegetative growthbfr1Δ, trr1Δ, caf5Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in hba1 Ran GTPase binding protein Hba1
FYPO affected by mutation in int6 eIF3e subunit Int6
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3
FYPO affected by mutation in prr1 transcription factor Prr1
FYPO affected by mutation in sty1 MAP kinase Sty1
FYPO affected by mutation in trr1 thioredoxin reductase Trr1
FYPO affected by mutation in trx1 cytosolic thioredoxin Trx1
FYPO affected by mutation in wis1 MAP kinase kinase Wis1
GO regulated by pap1 transcription factor Pap1/Caf3
GO regulated by sty1 MAP kinase Sty1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4189144..4189967, 4190267..4191448
Intron4189968..4190266
mRNA4189144..4191448
5' UTR4189144..4189966PMID:21511999
CDS4189967..4189967, 4190267..4191234
3' UTR4191235..4191448PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07992 Pfam IPR023753 FAD/NAD(P)-binding domain 5 303 8
PS00573 Prosite Patterns IPR008255 Pyridine nucleotide-disulphide oxidoreductase, class-II, active site 142 164 1
PTHR22912:SF167 HMMPANTHER 1 317 1
PTHR22912 HMMPANTHER 1 317 6
3.50.50.60 Gene3D IPR023753 FAD/NAD(P)-binding domain 250 319 29
3.50.50.60 Gene3D IPR023753 FAD/NAD(P)-binding domain 2 126 29
3.50.50.60 Gene3D IPR023753 FAD/NAD(P)-binding domain 127 249 29
SSF51905 SuperFamily IPR023753 FAD/NAD(P)-binding domain 1 315 26
PR00368 PRINTS 156 174 7
PR00368 PRINTS 268 290 7
PR00368 PRINTS 241 257 7
PR00368 PRINTS 112 130 7
PR00368 PRINTS 6 25 7
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 135 147 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 113 121 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 278 296 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 64 74 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 204 220 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 5 27 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 243 264 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 152 176 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 43 58 1
TIGR01292 TIGRFAM IPR005982 Thioredoxin reductase 5 315 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.51 Da
Charge -7.00
Codon Adaptation Index 0.69
Isoelectric point 4.96
Molecular weight 34.62 kDa
Number of residues 322
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during cellular response to thiabendazoleS192
S165, S192, S279
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S192 PMID:18257517
IDA S165 PMID:25720772
IDA S192 PMID:25720772
IDA S279 PMID:25720772
O-phospho-L-threonine 1085
present during mitotic M phaseT321
T278, T321
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T321 PMID:21712547
IDA T278 PMID:25720772
IDA T321 PMID:25720772
experimental evidence T321 PMID:24763107
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0071500reporter gene assay evidencePMID:22279963
increased during GO:0070301reporter gene assay evidencePMID:15135546
increased during GO:0036245reporter gene assay evidencePMID:15135546
increased during GO:0071288reporter gene assay evidencePMID:15135546
increased during GO:0071288reporter gene assay evidencePMID:16554715
increased during GO:0072715reporter gene assay evidencePMID:15135546
increased during GO:0036245reporter gene assay evidencePMID:16554715
increased during GO:0071275reporter gene assay evidencePMID:15135546
RNA levelpresent during GO:0072690Northern assay evidencePMID:10348908
increased during GO:0070301Northern assay evidencePMID:10348908
increased during GO:0034599expression microarray evidencePMID:12529438
increased during GO:0070301Northern assay evidencePMID:18758731
increased during GO:0070301Northern assay evidencePMID:9585505
present during GO:0072690Northern assay evidencePMID:18235227
increased during GO:0097238Northern assay evidencePMID:16141205
increased during GO:0036245Northern assay evidencePMID:15135546
increased during GO:0070301Northern assay evidencePMID:23115244
increased during GO:0070301Northern assay evidencePMID:18235227
present during GO:0072690Northern assay evidencePMID:9585505

Quantitative Gene Expression

View graphical display of gene expression data for trr1 (SPBC3F6.03)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
165920during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
147995during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
161013during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
161464during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
152648during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
100481.1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
45302.81during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
12during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in bacteria1005
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC3F6.03 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withtrr1thioredoxin reductase Trr1 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC3F6.03 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetically rescuescaf5spermine family transmembrane transporter Caf5 Synthetic RescuePMID:19672306
synthetically rescuesbfr1brefeldin A transmembrane transporter Bfr1 Synthetic RescuePMID:19672306
synthetically rescuestpx1thioredoxin peroxidase Tpx1 Synthetic RescuePMID:24521463
rescuestpx1thioredoxin peroxidase Tpx1 Phenotypic SuppressionPMID:24268782
rescuescdc42Rho family GTPase Cdc42 Phenotypic SuppressionPMID:10628977
synthetic growth defect withcaf5spermine family transmembrane transporter Caf5 Synthetic Growth DefectPMID:19672306
phenotype enhanced bytxl1thioredoxin-like I protein Txl1 Phenotypic EnhancementPMID:23121505
phenotype enhanced bytrx1cytosolic thioredoxin Trx1 Phenotypic EnhancementPMID:23121505
synthetic lethal withtpx1thioredoxin peroxidase Tpx1 Synthetic LethalityPMID:24521463
synthetic lethal withctt1catalase Synthetic LethalityPMID:24521463
External References
Database Identifier Description
NBRP SPBC3F6.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC3F6.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC3F6.03 BioGRID Interaction Datasets
Expression Viewer SPBC3F6.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC3F6.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC3F6.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC3F6.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC3F6.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC3F6.03 Transcriptome Viewer (Bähler Lab)
GEO SPBC3F6.03 GEO profiles
PInt SPBC3F6.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC3F6.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC3F6.03 Fission yeast phenotypic data & analysis
Cyclebase SPBC3F6.03.1 Cell Cycle Data
IntEnz1.8.1.9Integrated relational Enzyme database
Rhea1.8.1.9Annotated reactions database
SPD / RIKEN41/41H04Orfeome Localization Data
UniProtKB/SwissProtQ92375Thioredoxin reductase
ModBaseQ92375Database of comparative protein structure models
STRINGQ92375Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018848thioredoxin reductase Trr1
European Nucleotide ArchiveAAC49569.1ENA Protein Mapping
European Nucleotide ArchiveAAN01228.1ENA Protein Mapping
European Nucleotide ArchiveCAA17692.1ENA Protein Mapping
KEGG00240+1.8.1.9Pyrimidine metabolism
KEGG00450+1.8.1.9Selenocompound metabolism
UniParcUPI00001376D0UniProt Archive

Literature for trr1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016