trr1 (SPBC3F6.03)


Gene Standard Nametrr1 Characterisation Statuspublished
Systematic IDSPBC3F6.03 Feature Typeprotein coding
Synonymscaf4 Name Description
Productthioredoxin reductase Trr1 Product Size322aa, 34.62 kDa
Genomic Location Chromosome II, 4189144-4191448 (2305nt); CDS:4189967-4191234 (1268nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0050660flavin adenine dinucleotide bindingIEAIPR001327GO_REF:000000234
GO:0005515protein bindingIPItrx1PMID:17409354861
GO:0004791thioredoxin-disulfide reductase activityIDAPMID:174093541
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0019430removal of superoxide radicalsIEAIPR005982GO_REF:00000029
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISSUniProtKB:P29509GO_REF:00000014201
GO:0005829cytosolIDAPMID:168233722316
GO:0005739mitochondrionISSUniProtKB:P38816GO_REF:0000001758
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaytrr1ΔNullPECO:0000102, PECO:0000137PMID:2452146399
FYPO:0001753normal anaerobic cell population growthCell growth assaytrr1ΔNullPECO:0000102, PECO:0000137PMID:245214637
FYPO:0000067resistance to brefeldin ACell growth assaycaf4-83Not specifiedPMID:989491312
FYPO:0000073resistance to caffeineCell growth assaycaf4-83Not specifiedPECO:0000137, PECO:0000102PMID:921179019
Cell growth assaytrr1ΔNullPECO:0000102, PECO:0000137PMID:24521463
expressivity FYPO_EXT:0000001Cell growth assaytrr1ΔNullPECO:0000137PMID:19672306
FYPO:0001103resistance to hydrogen peroxideCell growth assaycaf4-83Not specifiedPMID:1475854160
FYPO:0002312resistance to ionizing radiationCell growth assaycaf4-83Not specifiedPMID:92117902
FYPO:0000268sensitive to UV during vegetative growthCell growth assaycaf4-83Not specifiedPECO:0000137PMID:9211790117
Cell growth assaytrr1ΔNullPECO:0000137, PECO:0000102PMID:9894913
FYPO:0001987sensitive to high pHCell growth assaytrr1ΔNullPECO:0000126PMID:98949133
Cell growth assaycaf4-83Not specifiedPECO:0000126PMID:9894913
Cell growth assaycaf4-83Not specifiedPECO:0000103, PECO:0000137PMID:9211790
FYPO:0000087sensitive to hydrogen peroxide121
expressivity FYPO_EXT:0000001Cell growth assaytrr1ΔNullPECO:0000137PMID:19672306
Cell growth assaytrr1ΔNullPECO:0000102, PECO:0000137PMID:24521463
FYPO:0001234slow vegetative cell population growthCell growth assaycaf4-83Not specifiedPECO:0000103, PECO:0000126PMID:921179054
Cell growth assaycaf4-83Not specifiedPECO:0000103, PECO:0000137PMID:9211790
FYPO:0002060viable vegetative cell populationCell growth assaytrr1ΔNullPMID:98949133760
Microscopytrr1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopytrr1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000584decreased sporulationCell growth assaycaf4-83Not specifiedPMID:921179042
FYPO:0000219increased protein oxidation4
affecting pap1Western blot assaytrr1ΔNullPMID:24521463
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopytrr1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:10348908
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:12100563
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:18758731
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:9585505
FYPO affected by mutation in prr1 transcription factor Prr1 PMID:10348908
FYPO affected by mutation in sty1 MAP kinase Sty1 PMID:9585505
FYPO affected by mutation in trx1 cytosolic thioredoxin Trx1 PMID:18758731
GO regulated by sty1 MAP kinase Sty1 PMID:9585505
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
141891444189967
241902674191448

UTRs

Region Coordinates Reference
five_prime_UTR4189144..4189966PMID:21511999
three_prime_UTR4191235..4191448PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07992 Pfam IPR023753 Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain 5 291 9
PF00070 Pfam IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain 157 224 6
PS00573 Prosite Patterns IPR008255 Pyridine nucleotide-disulphide oxidoreductase, class-II, active site 142 164 1
PTHR22912:SF48 HMMPANTHER 1 322 1
PTHR22912 HMMPANTHER 1 322 4
3.50.50.60 Gene3D 2 126 29
3.50.50.60 Gene3D 127 249 29
3.50.50.60 Gene3D 250 319 29
SSF51905 SuperFamily 1 315 26
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 156 174 7
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 112 130 7
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 64 74 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 135 147 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 278 296 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 113 121 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 152 176 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 204 220 1
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 241 257 7
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 268 290 7
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6 25 7
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 43 58 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 5 27 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 243 264 1
TIGR01292 tigrfam IPR005982 Thioredoxin reductase 5 315 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.51 Da
Charge -7.00
Isoelectric point 4.96
Molecular weight 34.62 kDa
Number of residues 322
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS192PMID:182575171663
present during cellular response to thiabendazole
MOD:00047O-phospho-L-threonineT321PMID:24763107682
present during mitotic M phaseT321PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171915
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0070301Northern assay evidencePMID:18758731
increased during GO:0070301Northern assay evidencePMID:23115244
increased during GO:0070301Northern assay evidencePMID:9585505
present during GO:0072690Northern assay evidencePMID:9585505
increased during GO:0034599expression microarray evidencePMID:12529438
increased during GO:0070301Northern assay evidencePMID:10348908
present during GO:0072690Northern assay evidencePMID:10348908

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
45302.81during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
100481.1during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
147995during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
152648during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
161013during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
161464during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
165920during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
12during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
txl1thioredoxin-like I protein Txl1 Phenotypic EnhancementPMID:23121505
trx1cytosolic thioredoxin Trx1 Phenotypic EnhancementPMID:23121505
cdc42Rho family GTPase Cdc42 Phenotypic SuppressionPMID:10628977
caf5spermine family transmembrane transporter Caf5 Phenotypic SuppressionPMID:19672306
bfr1brefeldin A efflux transporter Bfr1 Phenotypic SuppressionPMID:19672306
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
trr1thioredoxin reductase Trr1 Two-hybridPMID:23695164
External References
Database Identifier Description
NBRP SPBC3F6.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC3F6.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC3F6.03 BioGRID Interaction Datasets
Expression Viewer SPBC3F6.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC3F6.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC3F6.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC3F6.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC3F6.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC3F6.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC3F6.03 Cell Cycle Data
GEO SPBC3F6.03 GEO profiles
PInt SPBC3F6.03 Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz1.8.1.9Integrated relational Enzyme database
Rhea1.8.1.9Annotated reactions database
EntrezGene3361358thioredoxin reductase Trr1
WikiGene3361358thioredoxin reductase Trr1
SPD / RIKEN41/41H04Orfeome Localization Data
UniProtKB/SwissProtQ92375Thioredoxin reductase
ModBaseQ92375Database of comparative protein structure models
STRINGQ92375Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018848thioredoxin reductase Trr1
RefSeq mRNANM_001022668972h- thioredoxin reductase Trr1 (trr1), mRNA
European Nucleotide ArchiveAAC49569ENA Protein Mapping
European Nucleotide ArchiveAAC49569.1ENA Protein Mapping
European Nucleotide ArchiveAAN01228ENA Protein Mapping
European Nucleotide ArchiveAAN01228.1ENA Protein Mapping
European Nucleotide ArchiveCAA17692ENA Protein Mapping
European Nucleotide ArchiveCAA17692.1ENA Protein Mapping
UniParcUPI00001376D0UniProt Archive

Literature for trr1

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014