trr1 (SPBC3F6.03)


Gene Standard Nametrr1 Characterisation Statuspublished
Systematic IDSPBC3F6.03 Feature Typeprotein coding
Synonymscaf4, trxR Name Description
Productthioredoxin reductase Trr1 Product Size322aa, 34.62 kDa
Genomic Location Chromosome II, 4189144-4191448 (2305nt); CDS:4189967-4191234 (1268nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding854
Annotation ExtensionEvidenceWith/FromReference
thioredoxin-disulfide reductase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
removal of superoxide radicals8
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4208
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
mitochondrion761
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth on glucose carbon sourcetrr1ΔNull247
normal anaerobic cell population growthtrr1ΔNull8
resistance to aluminiumtrr1+ (wild type)Overexpression3
resistance to brefeldin Acaf4-83Not specified19
resistance to caffeinecaf4-83Not specified26
trr1ΔNull
resistance to hydrogen peroxidecaf4-83Not specified73
resistance to ionizing radiationcaf4-83Not specified2
resistance to mercurytrr1+ (wild type)Overexpression7
sensitive to high pHcaf4-83Not specified3
trr1ΔNull
sensitive to hydrogen peroxidetrr1ΔNull137
sensitive to UV during vegetative growthcaf4-83Not specified136
trr1ΔNull
slow vegetative cell population growthcaf4-83Not specified321
viable vegetative cell populationtrr1ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
decreased protein level during cellular response to menadione4
affecting trr1pYUTR40 (-186--499)Not specified
decreased protein level during vegetative growth91
affecting trr1pYUTR30 (-499--891)Not specified
decreased sporulationcaf4-83Not specified70
increased protein oxidation during vegetative growth4
affecting pap1trr1ΔNull
increased thioredoxin-disulfide reductase activitytrr1+ (wild type)Overexpression1
viable vegetative cell with normal cell morphologytrr1ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in int6 eIF3e subunit Int6 PMID:11071922
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:22279963
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:15135546
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:12100563
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:16141205
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:16554715
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:18758731
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:10348908
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:9585505
FYPO affected by mutation in prr1 transcription factor Prr1 PMID:10348908
FYPO affected by mutation in sty1 MAP kinase Sty1 PMID:15135546
FYPO affected by mutation in sty1 MAP kinase Sty1 PMID:9585505
FYPO affected by mutation in trr1 thioredoxin reductase Trr1 PMID:16554715
FYPO affected by mutation in trx1 cytosolic thioredoxin Trx1 PMID:18758731
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:15135546
GO regulated by pap1 transcription factor Pap1/Caf3 PMID:9585505
GO regulated by sty1 MAP kinase Sty1 PMID:9585505
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4189144..4189967, 4190267..4191448
mRNA4189144..4191448
5' UTR4189144..4189966PMID:21511999
CDS4189967..4189967, 4190267..4191234
3' UTR4191235..4191448PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00070 Pfam IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain 157 224 6
PF07992 Pfam IPR023753 Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain 5 291 9
PS00573 Prosite Patterns IPR008255 Pyridine nucleotide-disulphide oxidoreductase, class-II, active site 142 164 1
PTHR22912 HMMPANTHER 1 322 4
PTHR22912:SF48 HMMPANTHER 1 322 1
3.50.50.60 Gene3D 127 249 29
3.50.50.60 Gene3D 2 126 29
3.50.50.60 Gene3D 250 319 29
SSF51905 SuperFamily 1 315 26
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 112 130 7
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 156 174 7
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 268 290 7
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 241 257 7
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 135 147 1
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6 25 7
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 278 296 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 113 121 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 204 220 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 5 27 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 43 58 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 243 264 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 64 74 1
PR00469 PRINTS IPR000103 Pyridine nucleotide-disulphide oxidoreductase, class-II 152 176 1
TIGR01292 tigrfam IPR005982 Thioredoxin reductase 5 315 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.51 Da
Charge -7.00
Isoelectric point 4.96
Molecular weight 34.62 kDa
Number of residues 322
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during cellular response to thiabendazoleS192
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S192 PMID:18257517
O-phospho-L-threonineT321 698
present during mitotic M phaseT321
Annotation ExtensionEvidenceResidueReference
experimental evidence T321 PMID:24763107
present during mitotic M phase experimental evidence T321 PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0071288reporter gene assay evidencePMID:16554715
increased during GO:0036245reporter gene assay evidencePMID:15135546
increased during GO:0036245reporter gene assay evidencePMID:16554715
increased during GO:0072715reporter gene assay evidencePMID:15135546
increased during GO:0071275reporter gene assay evidencePMID:15135546
increased during GO:0071500reporter gene assay evidencePMID:22279963
increased during GO:0071288reporter gene assay evidencePMID:15135546
increased during GO:0070301reporter gene assay evidencePMID:15135546
RNA levelpresent during GO:0072690Northern assay evidencePMID:9585505
increased during GO:0070301Northern assay evidencePMID:9585505
increased during GO:0034599expression microarray evidencePMID:12529438
increased during GO:0097238Northern assay evidencePMID:16141205
increased during GO:0036245Northern assay evidencePMID:15135546
increased during GO:0070301Northern assay evidencePMID:10348908
present during GO:0072690Northern assay evidencePMID:10348908
increased during GO:0070301Northern assay evidencePMID:18758731
increased during GO:0070301Northern assay evidencePMID:23115244

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
165920during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
147995during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
161013during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
161464during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
100481.1during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
152648during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
45302.81during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
12during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
binds activation domain construct withtrr1thioredoxin reductase Trr1 Two-hybridPMID:23695164
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescued bybfr1brefeldin A transmembrane transporter Bfr1 Phenotypic SuppressionPMID:19672306
rescued bycaf5spermine family transmembrane transporter Caf5 Phenotypic SuppressionPMID:19672306
rescuescdc42Rho family GTPase Cdc42 Phenotypic SuppressionPMID:10628977
synthetic lethal withctt1catalase Synthetic LethalityPMID:24521463
synthetic lethal withtpx1thioredoxin peroxidase Tpx1 Synthetic LethalityPMID:24521463
synthetically rescuestpx1thioredoxin peroxidase Tpx1 Synthetic RescuePMID:24521463
phenotype enhanced bytrx1cytosolic thioredoxin Trx1 Phenotypic EnhancementPMID:23121505
phenotype enhanced bytxl1thioredoxin-like I protein Txl1 Phenotypic EnhancementPMID:23121505
External References
Database Identifier Description
NBRP SPBC3F6.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC3F6.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC3F6.03 BioGRID Interaction Datasets
Expression Viewer SPBC3F6.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC3F6.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC3F6.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC3F6.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC3F6.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC3F6.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC3F6.03 Cell Cycle Data
GEO SPBC3F6.03 GEO profiles
PInt SPBC3F6.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC3F6.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC3F6.03 Fission yeast phenotypic data & analysis
IntEnz1.8.1.9Integrated relational Enzyme database
Rhea1.8.1.9Annotated reactions database
SPD / RIKEN41/41H04Orfeome Localization Data
UniProtKB/SwissProtQ92375Thioredoxin reductase
ModBaseQ92375Database of comparative protein structure models
STRINGQ92375Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018848thioredoxin reductase Trr1
RefSeq mRNANM_001022668972h- thioredoxin reductase Trr1 (trr1), mRNA
European Nucleotide ArchiveAAC49569.1ENA Protein Mapping
European Nucleotide ArchiveAAN01228.1ENA Protein Mapping
European Nucleotide ArchiveCAA17692.1ENA Protein Mapping
KEGG_Enzyme00240+1.8.1.9Pyrimidine metabolism
KEGG_Enzyme00450+1.8.1.9Selenocompound metabolism
UniParcUPI00001376D0UniProt Archive

Literature for trr1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015