ypt7 (SPBC405.04c)


Gene Standard Nameypt7 Characterisation Statuspublished
Systematic IDSPBC405.04c Feature Typeprotein coding
Synonyms Name Description
ProductGTPase Ypt7 Product Size205aa, 22.99 kDa
Genomic Location Chromosome II, 3148452-3147096 (1357nt); CDS:3148230-3147478 (753nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding112
Annotation ExtensionEvidenceWith/FromReference
GTPase activity62
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
Golgi to vacuole transport18
Annotation ExtensionEvidenceWith/FromReference
intracellular protein transport342
Annotation ExtensionEvidenceWith/FromReference
positive regulation of vacuole fusion, non-autophagic2
Annotation ExtensionEvidenceWith/FromReference
regulation of endocytosis14
Annotation ExtensionEvidenceWith/FromReference
small GTPase mediated signal transduction152
Annotation ExtensionEvidenceWith/FromReference
vacuolar acidification16
Annotation ExtensionEvidenceWith/FromReference
vacuole fusion, non-autophagic21
Annotation ExtensionEvidenceWith/FromReference
vesicle fusion with vacuole1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
vacuolar membrane109
Annotation ExtensionEvidenceWith/FromReference
vacuole185
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to 5-fluorouracilypt7ΔNull271
viable vegetative cell populationypt7ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
abnormal prospore membrane formationypt7ΔNull27
abnormal sporulation resulting in formation of ascus containing anucleate sporesypt7ΔNull6
abnormal sporulation resulting in formation of ascus with fewer than four sporesypt7ΔNull60
decreased spore germinationypt7ΔNull31
small sporesypt7ΔNull7
small vacuoles during sporulationypt7ΔNull1
small vacuoles present in greater numbers during vegetative growthunnamed (unrecorded)19
viable vegetative cell with normal cell morphologyypt7ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3148452..3148150, 3148076..3147753, 3147690..3147096
mRNA3148452..3147096
5' UTR3148452..3148231PMID:21511999
CDS3148230..3148150, 3148076..3147753, 3147690..3147478
3' UTR3147477..3147096PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00071 Pfam IPR001806 Small GTPase superfamily 10 175 18
SM00173 SMART IPR020849 Small GTPase superfamily, Ras type 6 178 19
SM00175 SMART IPR003579 Small GTPase superfamily, Rab type 9 178 22
SM00174 SMART IPR003578 Small GTPase superfamily, Rho type 11 178 19
SM00176 SMART IPR002041 Ran GTPase 14 200 13
PS51419 Prosite Profiles 4 205 9
PTHR24073 HMMPANTHER 1 205 9
PTHR24073:SF57 HMMPANTHER 1 205 2
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 3 185 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 4 180 294
PR00449 PRINTS IPR001806 Small GTPase superfamily 50 72 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 116 129 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 153 175 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 32 48 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 9 30 19
TIGR00231 tigrfam IPR005225 Small GTP-binding protein domain 9 172 35

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.17 Da
Charge -6.00
Isoelectric point 4.72
Molecular weight 22.99 kDa
Number of residues 205
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
62432during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
63646during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
58951during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
67228during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
59964during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4715.94during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
14433.77during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byvps26retromer complex subunit Vps26 Affinity Capture-WesternPMID:21062894
affinity captured byvps41HOPS complex subunit Vps41 (predicted) Affinity Capture-WesternPMID:21062894
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescuesbtn1battenin CLN3 family protein Phenotypic SuppressionPMID:16291725
External References
Database Identifier Description
NBRP SPBC405.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC405.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC405.04c BioGRID Interaction Datasets
Expression Viewer SPBC405.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC405.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC405.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC405.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC405.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC405.04c Transcriptome Viewer (Bähler Lab)
GEO SPBC405.04c GEO profiles
PInt SPBC405.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC405.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC405.04c Fission yeast phenotypic data & analysis
Cyclebase SPBC405.04c.1 Cell Cycle Data
SPD / RIKEN31/31H05Orfeome Localization Data
UniProtKB/SwissProtO94655GTP-binding protein ypt7
ModBaseO94655Database of comparative protein structure models
STRINGO94655Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596307GTPase Ypt7
RefSeq mRNANM_001022229972h- GTPase Ypt7 (ypt7), mRNA
European Nucleotide ArchiveCAB38603.1ENA Protein Mapping
UniParcUPI000013BE48UniProt Archive

Literature for ypt7

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015