atg16 (SPBC405.05)


Gene Standard Nameatg16 Characterisation Statuspublished
Systematic IDSPBC405.05 Feature Typeprotein coding
Synonyms Name Description
Productautophagy protein Atg16 Product Size143aa, 16.89 kDa
Genomic Location Chromosome II, 3148510-3149656 (1147nt); CDS:3148752-3149183 (432nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
C-terminal protein lipidation3
Annotation ExtensionEvidenceWith/FromReference
CVT pathway20
Annotation ExtensionEvidenceWith/FromReference
macroautophagy27
Annotation ExtensionEvidenceWith/FromReference
meiotic cell cycle240
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
pre-autophagosomal structure23
Annotation ExtensionEvidenceWith/FromReference
pre-autophagosomal structure membrane19
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyatg16Δ269
viable vegetative cell populationatg16Δ3831

Cell Phenotype

Term NameGenotypesCount
abnormal meiosisatg16Δ121
abolished autophagyatg16Δ22
decreased protein localization to pre-autophagosomal structure12
affecting atg8atg16Δ
viable vegetative cell with normal cell morphologyatg16Δ3099
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3148510..3149656
5' UTR3148510..3148751SPD255
3' UTR3149184..3149656SPD255
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
Coil ncoils Predicted coiled-coil protein (DUF2205) 112 139 1050
Coil ncoils Predicted coiled-coil protein (DUF2205) 70 104 1050

View domain organization at Pfam

Protein Properties

Ave. residue weight 118.13 Da
Charge -4.50
Codon Adaptation Index 0.34
Isoelectric point 4.79
Molecular weight 16.89 kDa
Number of residues 143
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1675
present during mitotic M phaseS57
present during mitotic M phaseS51
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S51 PMID:21712547
present during mitotic M phase experimental evidence S57 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for atg16 (SPBC405.05)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.49during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi PMID:239507354604
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPBC405.05 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withtim13TIM22 inner membrane protein import complex subunit Tim13 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
negative genetic interaction withppk32serine/threonine protein kinase Ppk32 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withfin1serine/threonine protein kinase, NIMA related Fin1 Negative GeneticPMID:22681890
negative genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withSPAC19B12.11czinc finger protein, human ZNF593 ortholog Negative GeneticPMID:22681890
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withhul5HECT-type ubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withplb1phospholipase B homolog Plb1 Negative GeneticPMID:22681890
negative genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withdad1DASH complex subunit Dad1 Positive GeneticPMID:22681890
positive genetic interaction withswd3WD repeat protein Swd3 Positive GeneticPMID:22681890
positive genetic interaction withrcd1RNA-binding protein, CCR4-NOT complex subunit Rcd1 Positive GeneticPMID:22681890
positive genetic interaction withSPBC1861.07elongin C (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
positive genetic interaction withrga3Rho-type GTPase activating protein Rga3 Positive GeneticPMID:22681890
positive genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
positive genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
positive genetic interaction withSPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC405.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC405.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC405.05 BioGRID Interaction Datasets
Expression Viewer SPBC405.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC405.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC405.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC405.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC405.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC405.05 Transcriptome Viewer (Bähler Lab)
GEO SPBC405.05 GEO profiles
PInt SPBC405.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC405.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC405.05 Fission yeast phenotypic data & analysis
Cyclebase SPBC405.05.1 Cell Cycle Data
SPD / RIKEN03/03F02Orfeome Localization Data
UniProtKB/SwissProtO94656Autophagy protein 16
ModBaseO94656Database of comparative protein structure models
STRINGO94656Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596308sequence orphan
RefSeq mRNANM_001022230972h- sequence orphan (SPBC405.05), mRNA
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveD89255ENA EMBL mapping
European Nucleotide ArchiveBAA13916ENA Protein Mapping
European Nucleotide ArchiveCAB38604ENA Protein Mapping
UniParcUPI000006C86AUniProt Archive

Literature for atg16

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016