atg16 (SPBC405.05)

Gene Standard Nameatg16 Characterisation Statuspublished
Systematic IDSPBC405.05 Feature Typeprotein coding
Synonyms Name Description
Productautophagy associated protein Atg16 Product Size143aa, 16.89 kDa
Genomic Location Chromosome II, 3148510-3149656 (1147nt); CDS:3148752-3149183 (432nt)

Ensembl Gene Location
GO Biological Process

GO Slim Terms


Term NameCount
C-terminal protein lipidation3
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
meiotic cell cycle234
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
pre-autophagosomal structure23
Annotation ExtensionEvidenceWith/FromReference
pre-autophagosomal structure membrane19
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyatg16Δ276
viable vegetative cell populationatg16Δ3862

Cell Phenotype

Term NameGenotypesCount
abnormal meiosisatg16Δ134
abolished autophagyatg16Δ22
decreased protein localization to pre-autophagosomal structure12
affects localization of atg8atg16Δ
viable vegetative cell with normal cell morphologyatg16Δ3103
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR3148510..3148751SPD255
3' UTR3149184..3149656SPD255
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
Coil ncoils Predicted coiled-coil protein (DUF2205) 112 139 1049
Coil ncoils Predicted coiled-coil protein (DUF2205) 70 104 1049

View domain organization at Pfam

Protein Properties

Ave. residue weight 118.13 Da
Charge -4.50
Codon Adaptation Index 0.34
Isoelectric point 4.79
Molecular weight 16.89 kDa
Number of residues 143

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2293
present during mitotic M phaseS51
present during mitotic M phaseS57
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S51 PMID:21712547
present during mitotic M phase experimental evidence S57 PMID:21712547
IDA S48 PMID:25720772
O-phospho-L-threonineT46 1087
Annotation ExtensionEvidenceResidueReference
IDA T46 PMID:25720772
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for atg16 (SPBC405.05)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
0.49during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
conserved in fungi PMID:239507354608
conserved in eukaryotes4516

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC405.05 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byimp1importin alpha Affinity Capture-MSPMID:25963819
affinity capturesatg5autophagy associated protein Atg5 Affinity Capture-WesternPMID:23950735
binds activation domain construct withatg16autophagy associated protein Atg16 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC405.05 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withvps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
negative genetic interaction withpng2ING family histone acetyltransferase complex PHD-type zinc finger subunit Png2 Negative GeneticPMID:22681890
negative genetic interaction withckb2CK2 family regulatory subunit Ckb2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withnup40nucleoporin Nup40 Negative GeneticPMID:22681890
positive genetic interaction withswd3WD repeat protein Swd3 Positive GeneticPMID:22681890
positive genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
positive genetic interaction withrem1meiosis-specific cyclin Rem1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC405.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC405.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC405.05 BioGRID Interaction Datasets
Expression Viewer SPBC405.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC405.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC405.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC405.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC405.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC405.05 Transcriptome Viewer (Bähler Lab)
GEO SPBC405.05 GEO profiles
PInt SPBC405.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC405.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC405.05 Fission yeast phenotypic data & analysis
Cyclebase SPBC405.05.1 Cell Cycle Data
SPD / RIKEN03/03F02Orfeome Localization Data
UniProtKB/SwissProtO94656Autophagy protein 16
ModBaseO94656Database of comparative protein structure models
STRINGO94656Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596308sequence orphan
RefSeq mRNANM_001022230972h- sequence orphan (SPBC405.05), mRNA
European Nucleotide ArchiveD89255ENA EMBL mapping
European Nucleotide ArchiveCAB38604.1ENA Protein Mapping
UniParcUPI000006C86AUniProt Archive

Literature for atg16

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017