wis1 (SPBC409.07c)


Gene Standard Namewis1 Characterisation Statuspublished
Systematic IDSPBC409.07c Feature Typeprotein coding
Synonymssmf2, spc2 Name DescriptionWIn Suppressing
ProductMAP kinase kinase Wis1 Product Size605aa, 64.76 kDa
Genomic Location Chromosome II, 1146633-1143731 (2903nt); CDS:1145784-1143967 (1818nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
MAP kinase kinase activity3
Annotation ExtensionEvidenceWith/FromReference
non-membrane spanning protein tyrosine kinase activity1
Annotation ExtensionEvidenceWith/FromReference
protein binding848
protein serine/threonine kinase activity112
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
activation of MAPK activity3
Annotation ExtensionEvidenceWith/FromReference
G1 cell cycle arrest in response to nitrogen starvation7
Annotation ExtensionEvidenceWith/FromReference
G1 to G0 transition9
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade25
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade in response to starvation2
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade involved in osmosensory signaling pathway3
Annotation ExtensionEvidenceWith/FromReference
mitotic cell cycle arrest in response to nitrogen starvation7
Annotation ExtensionEvidenceWith/FromReference
peptidyl-tyrosine phosphorylation11
Annotation ExtensionEvidenceWith/FromReference
positive regulation of G2/M transition of mitotic cell cycle12
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription factor import into nucleus in response to oxidative stress2
Annotation ExtensionEvidenceWith/FromReference
protein autophosphorylation16
Annotation ExtensionEvidenceWith/FromReference
protein phosphorylation109
Annotation ExtensionEvidenceWith/FromReference
regulation of cell shape involved in G1 to G0 transition2
Annotation ExtensionEvidenceWith/FromReference
regulation of G2/M transition of mitotic cell cycle62
Annotation ExtensionEvidenceWith/FromReference
regulation of translation in response to stress10
Annotation ExtensionEvidenceWith/FromReference
stress-activated MAPK cascade9
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
abolished cell population growth on glycerol carbon sourcewis1ΔNull65
decreased bipolar indexwis1ΔNull27
decreased cell population growth at high temperatureAA (S469A, T473A)Endogenous151
AAA (S469A, S471A, T473A)Endogenous
K349R (K349R)Overexpression
decreased cell population growth during nitrogen starvationwis1-558 (G461D)35
wis1-982 (F307S)
decreased cell population growth on glucose carbon sourcewis1ΔNull154
decreased conjugation frequencywis1ΔNull39
decreased mating efficiencyAA (S469A, T473A)Endogenous247
AAA (S469A, S471A, T473A)Endogenous
wis1-DD (S469D, T473D)Not specified
wis1ΔNull
expressivity FYPO_EXT:0000001wis1ΔNull
decreased septation indexwis1ΔNull20
increased monopolar indexwis1ΔNull35
inviable vegetative cell populationnot recorded (wild type)1427
wis1+ (wild type)Overexpression
loss of viability at high temperaturewis1ΔNull35
loss of viability in stationary phasewis1ΔNull52
normal cell population growth at high temperatureAAA (S469A, S471A, T473A)Overexpression40
normal growth on glucose carbon sourcewis1ΔNull189
normal vegetative cell population growth rateAA (S469A, T473A)Endogenous57
AAA (S469A, S471A, T473A)Endogenous
wis1ΔNull
resistance to caffeinewis1DD26
resistance to cobalt56
expressivity FYPO_EXT:0000002wis1ΔNull
resistance to hydrogen peroxidewis1DD72
sensitive to arsenatewis1ΔNull2
sensitive to bleomycinwis1ΔNull50
sensitive to caffeine108
expressivity FYPO_EXT:0000001wis1ΔNull
sensitive to heat shockwis1ΔNull6
sensitive to high osmolarityAA (S469A, T473A)Endogenous11
AAA (S469A, S471A, T473A)Endogenous
wis1ΔNull
sensitive to hydrogen peroxidewis1ΔNull137
expressivity FYPO_EXT:0000001wis1ΔNull
sensitive to ionizing radiationwis1ΔNull58
sensitive to miconazolenot recorded (wild type)37
sensitive to non-ionic osmotic stresswis1ΔNull12
sensitive to osmotic stresswis1AA (S469A, T473A)Endogenous97
sensitive to salt stresswis1ΔNull86
sensitive to sorbitolAA (S469A, T473A)Endogenous11
AAA (S469A, S471A, T473A)Endogenous
wis1ΔNull
sensitive to tacrolimus81
expressivity FYPO_EXT:0000002wis1ΔNull
sensitive to UV during vegetative growthwis1ΔNull133
viable vegetative cell populationwis1-DD (S469D, T473D)Endogenous3781
wis1ΔNull

Cell Phenotype

Term NameAlleleExpressionCount
abnormal cell cycle arrest in mitotic G2 phase during nitrogen starvationwis1-558 (G461D)9
wis1-982 (F307S)
abnormal chromatin organization during nitrogen starvationwis1-982 (F307S)Not specified3
abnormal microtubule cytoskeleton morphology during mitotic interphase186
penetrance FYPO_EXT:0000002wis1ΔNull
abnormal mitotic cell cyclewis1ΔNull794
abnormal mitotic cell cycle arrest in response to nitrogen starvation23
penetrance FYPO_EXT:0000001wis1ΔNull
abolished entry into G0wis1-558 (G461D)5
wis1-982 (F307S)
abolished protein autophosphorylation during vegetative growth7
affecting wis1K349R (K349R)Not specified
abolished protein kinase activity17
affecting wis1K349R (K349R)Not specified
affecting sty1wis1ΔNull
abolished protein localization to nucleus during cellular response to salt stress4
affecting srk1wis1-DD (S469D, T473D)Not specified
affecting sty1wis1ΔNull
abolished protein phosphorylation during cellular response to arsenic-containing substance1
affecting sty1wis1ΔNull
abolished protein phosphorylation during vegetative growth30
affecting sty1K349R (K349R)Not specified
abolished protein tyrosine phosphorylation4
affecting sty1wis1ΔNull
abolished protein tyrosine phosphorylation during cellular response to heat1
affecting sty1AAA (S469A, S471A, T473A)Endogenous
affecting sty1wis1ΔNull
affecting sty1wis1AA (S469A, T473A)Endogenous
abolished protein tyrosine phosphorylation during cellular response to hydrogen peroxide1
affecting sty1wis1ΔNull
affecting sty1wis1AA (S469A, T473A)Endogenous
abolished protein tyrosine phosphorylation during cellular response to osmotic stress1
affecting sty1AAA (S469A, S471A, T473A)Endogenous
affecting sty1wis1ΔNull
affecting sty1wis1AA (S469A, T473A)Endogenous
alpha,alpha-trehalase activity increase abolished during cellular response to hydrogen peroxidewis1ΔNull4
decreased cell cycle arrest in mitotic G1 phase in response to nitrogen starvationwis1ΔNull6
decreased cellular glycerol level during cellular response to salt stresswis1ΔNull2
decreased glutathione disulfide oxidoreductase activitywis1ΔNull4
decreased protein level during cellular response to glucose starvation3
affecting fbp1wis1ΔNull
decreased protein level during cellular response to nitrogen starvation15
affecting mei2wis1ΔNull
decreased protein level during vegetative growth80
affecting trr1wis1ΔNull
decreased protein phosphorylation58
affecting wis1AA (S469A, T473A)Not specified
affecting wis1AAA (S469A, S471A, T473A)Not specified
decreased protein phosphorylation during salt stress7
affecting srk1wis1-DD (S469D, T473D)Not specified
decreased protein phosphorylation during vegetative growth54
affecting atf1wis1ΔNull
decreased protein tyrosine phosphorylation during cellular response to heat1
affecting sty1AAA (S469A, S471A, T473A)Overexpression
decreased protein tyrosine phosphorylation during cellular response to hydrogen peroxide1
affecting sty1AAA (S469A, S471A, T473A)Overexpression
decreased RNA level during cellular response to glucose stimulus4
affecting mae2wis1 mutantNot specified
decreased RNA level during cellular response to heat7
affecting pyp2AAA (S469A, S471A, T473A)Overexpression
affecting gpd1AAA (S469A, S471A, T473A)Overexpression
affecting ctt1AAA (S469A, S471A, T473A)Overexpression
affecting pyp2K349R (K349R)Overexpression
affecting gpd1K349R (K349R)Overexpression
affecting ctt1K349R (K349R)Overexpression
affecting SPAC1486.01wis1ΔNull
decreased RNA level during cellular response to hydrogen peroxide20
affecting pyp2AAA (S469A, S471A, T473A)Overexpression
affecting gpd1AAA (S469A, S471A, T473A)Overexpression
affecting ctt1AAA (S469A, S471A, T473A)Overexpression
affecting pyp2K349R (K349R)Not specified
affecting gpd1K349R (K349R)Not specified
affecting ctt1K349R (K349R)Not specified
affecting sod1wis1ΔNull
affecting ntp1wis1ΔNull
decreased RNA level during cellular response to hypoxia5
affecting mae2wis1 mutantNot specified
decreased RNA level during cellular response to menadione4
affecting SPAC1486.01wis1ΔNull
decreased RNA level during cellular response to non-ionic osmotic stress3
affecting cta3wis1ΔNull
decreased RNA level during cellular response to salt stress12
affecting SPAC1486.01wis1ΔNull
affecting gpd1wis1ΔNull
affecting cta3wis1ΔNull
decreased RNA level during cellular response to UV10
affecting uvi15wis1ΔNull
decreased RNA level during nitrogen starvation34
affecting ste11wis1ΔNull
decreased RNA level during vegetative growth135
affecting gpd1wis1ΔNull
elongated cellwis1-558 (G461D)712
elongated cell during nitrogen starvationwis1-558 (G461D)18
wis1-982 (F307S)
enlarged nucleus during nitrogen starvationwis1-558 (G461D)2
wis1-982 (F307S)Endogenous
increased nuclear protein level during cellular response to hydrogen peroxide2
affecting pap1wis1+ (wild type)Overexpression
increased protein level87
affecting cdc13wis1-982 (F307S)
increased protein phosphorylation during vegetative growth31
affecting sty1wis1-DD (S469D, T473D)Endogenous
increased protein tyrosine phosphorylation during cellular response to heat1
affecting sty1wis1-DD (S469D, T473D)Endogenous
increased RNA level during vegetative growth171
affecting pyp2wis1+ (wild type)Overexpression
increased RNA level in stationary phase3
affecting sod1wis1ΔNull
normal protein kinase activity12
affecting wis1AAA (S469A, S471A, T473A)Not specified
normal protein level during vegetative growth47
affecting wis1AA (S469A, T473A)Endogenous
affecting wis1AAA (S469A, S471A, T473A)Endogenous
normal protein phosphorylation during cellular response to salt stress5
affecting pmk1wis1ΔNull
normal RNA level during cellular response to menadione4
affecting sod1wis1ΔNull
RNA absent from cell during nitrogen starvation13
affecting ste11wis1ΔNull
sterilewis1ΔNull54
swollen vegetative cellwis1+ (wild type)Overexpression49
vegetative cell lysiswis1+ (wild type)Overexpression82
viable elongated vegetative cellAA (S469A, T473A)Endogenous231
AAA (S469A, S471A, T473A)Endogenous
K349R (K349R)Overexpression
wis1ΔNull
penetrance FYPO_EXT:0000001, expressivity FYPO_EXT:0000001wis1ΔNull
wis1AA (S469A, T473A)Endogenous
viable small vegetative cellwis1+ (wild type)Overexpression38
wis1-DD (S469D, T473D)Endogenous
viable vegetative cell with abnormal cell shape472
penetrance FYPO_EXT:0000002wis1ΔNull
viable vegetative cell with normal cell morphologyAA (S469A, T473A)Endogenous3092
AAA (S469A, S471A, T473A)Endogenous
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in mcs4 response regulator Mcs4 PMID:23389634
FYPO affected by mutation in win1 MAP kinase kinase kinase Win1 PMID:9693384
FYPO affected by mutation in win1 MAP kinase kinase kinase Win1 PMID:23389634
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:9693384
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:9718372
GO regulated by win1 MAP kinase kinase kinase Win1 PMID:9693384
GO substrate of win1 MAP kinase kinase kinase Win1 PMID:9693384
GO substrate of wis4 MAP kinase kinase kinase Wis4 PMID:9718372
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
111466331143731

UTRs

Region Coordinates Reference
five_prime_UTR1146633..1145785PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 322 579 109
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 320 579 107
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 326 349 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 437 449 97
PS50011 Prosite Profiles IPR000719 Protein kinase domain 320 579 110
PTHR24360:SF3 HMMPANTHER 139 605 1
PTHR24360 HMMPANTHER 139 605 3
3.30.200.20 Gene3D 315 383 111
1.10.510.10 Gene3D 384 586 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 318 585 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.05 Da
Charge 13.50
Isoelectric point 9.40
Molecular weight 64.76 kDa
Number of residues 605
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during cellular response to thiabendazole, mitotic M phaseS129
present during mitotic M phaseS197
added by wis4469
present during mitotic M phaseS201
S129
Annotation ExtensionEvidenceResidueReference
added by wis4 IDA 469 PMID:9718372
experimental evidence S129 PMID:24763107
present during cellular response to thiabendazole IDA S129 PMID:18257517
present during mitotic M phase experimental evidence S129 PMID:21712547
present during mitotic M phase experimental evidence S197 PMID:21712547
present during mitotic M phase experimental evidence S201 PMID:21712547
O-phospho-L-threonineT132 693
added by wis4473
present during mitotic M phaseT132
Annotation ExtensionEvidenceResidueReference
added by wis4 IDA 473 PMID:9718372
experimental evidence T132 PMID:24763107
present during mitotic M phase experimental evidence T132 PMID:21712547
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:1756736

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
10153during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
12460during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
12150during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
12134during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4753.27during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
12418during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7210.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.1during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
wis1MAP kinase kinase Wis1 Biochemical ActivityPMID:9718372
mpr1histidine-containing response regulator phosphotransferase Mpr1 Two-hybridPMID:23695164
sty1MAP kinase Sty1 Affinity Capture-WesternPMID:12181336
Biochemical ActivityPMID:7501024
Two-hybridPMID:23695164
Biochemical ActivityPMID:9718372
Biochemical ActivityPMID:10398679
Affinity Capture-WesternPMID:9585506
win1MAP kinase kinase kinase Win1 Affinity Capture-WesternPMID:24255738
Biochemical ActivityPMID:9693384
Affinity Capture-LuminescencePMID:23389634
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
wis4MAP kinase kinase kinase Wis4 Biochemical ActivityPMID:9718372
Biochemical ActivityPMID:9321395
Affinity Capture-LuminescencePMID:23389634
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
nrd1RNA-binding protein Nrd1 Phenotypic SuppressionPMID:9671458
tup11transcriptional corepressor Tup11 Synthetic Growth DefectPMID:11238405
Phenotypic Suppression
ptc1protein phosphatase 2C Ptc1 Synthetic RescuePMID:7859738
leu13-isopropylmalate dehydrogenase Leu1 Phenotypic SuppressionPMID:15466417
pyp1tyrosine phosphatase Pyp1 Phenotypic EnhancementPMID:9321395
sds23PP2A-type phosphatase inhibitor Sds23/Moc1 Phenotypic EnhancementPMID:23640764
Dosage Lethality
lsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Phenotypic SuppressionPMID:22144909
cmk2MAPK-activated protein kinase Cmk2 Synthetic RescuePMID:11886858
ptc4protein phosphatase 2C Ptc4 Phenotypic EnhancementPMID:22139357
cdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic SuppressionPMID:1756736
Dosage Rescue
Synthetic LethalityPMID:9843572
Synthetic LethalityPMID:9136929
Synthetic LethalityPMID:8824588
Dosage RescuePMID:8557102
cut1separase/separin Synthetic RescuePMID:16861909
rcd1RNA-binding protein, CCR4-NOT complex subunit Rcd1 Phenotypic EnhancementPMID:9447985
srk1MAPK-activated protein kinase Srk1 Synthetic RescuePMID:12080074
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:1756736
sty1MAP kinase Sty1 Synthetic RescuePMID:9450957
Synthetic RescuePMID:9136929
Phenotypic SuppressionPMID:7501024
Phenotypic SuppressionPMID:8649397
Dosage RescuePMID:8557102
wis4MAP kinase kinase kinase Wis4 Synthetic RescuePMID:9450957
Synthetic RescuePMID:9718372
Synthetic RescuePMID:9321395
Synthetic RescuePMID:9614178
Dosage RescuePMID:9693384
Phenotypic Suppression
tup12transcriptional corepressor Tup12 Phenotypic SuppressionPMID:11238405
Synthetic Growth Defect
ppa1minor serine/threonine protein phosphatase Ppa1 Phenotypic SuppressionPMID:1756736
ptc3protein phosphatase 2c homolog 3 Synthetic RescuePMID:7859738
wee1M phase inhibitor protein kinase Wee1 Dosage RescuePMID:1756736
Phenotypic Suppression
cyr1adenylate cyclase Synthetic RescuePMID:15189983
gti1gluconate transmembrane transporter inducer Gti1 Phenotypic SuppressionPMID:9372449
mcs4response regulator Mcs4 Synthetic RescuePMID:9136929
Dosage Rescue
atf1transcription factor, Atf-CREB family Atf1 Synthetic RescuePMID:8824587
Phenotypic SuppressionPMID:15870269
pka1cAMP-dependent protein kinase catalytic subunit Pka1 Synthetic Growth DefectPMID:23640764
gpd2glycerol-3-phosphate dehydrogenase Gpd2 Dosage RescuePMID:7498541
win1MAP kinase kinase kinase Win1 Dosage RescuePMID:1756736
Synthetic RescuePMID:9450957
Dosage Rescue
Synthetic RescuePMID:9321395
Phenotypic SuppressionPMID:9693384
Dosage Rescue
sts5RNB-like protein Synthetic RescuePMID:8886983
pck2protein kinase C (PKC)-like Pck2 Phenotypic SuppressionPMID:9199286
gpd1glycerol-3-phosphate dehydrogenase Gpd1 Dosage RescuePMID:7498541
cdc37Hsp90 co-chaperone Cdc37 Synthetic RescuePMID:12861001
isp6vacuolar serine protease Isp6 Phenotypic EnhancementPMID:16550352
ran1serine/threonine protein kinase Ran1 Synthetic RescuePMID:8832415
Synthetic RescuePMID:8824588
mkh1MEK kinase (MEKK) Mkh1 Phenotypic EnhancementPMID:9199286
Phenotypic Suppression
byr1MAP kinase kinase Byr1 Synthetic Growth DefectPMID:8557102
ppa2serine/threonine protein phosphatase Ppa2 Phenotypic SuppressionPMID:1756736
External References
Database Identifier Description
NBRP SPBC409.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC409.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC409.07c BioGRID Interaction Datasets
Expression Viewer SPBC409.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC409.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC409.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC409.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC409.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC409.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC409.07c Cell Cycle Data
GEO SPBC409.07c GEO profiles
PInt SPBC409.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC409.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC409.07c Fission yeast phenotypic data & analysis
SPD / RIKEN33/33D12Orfeome Localization Data
PUBMED9135147
UniProtKB/SwissProtP33886Protein kinase wis1
ModBaseP33886Database of comparative protein structure models
STRINGP33886Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595457MAP kinase kinase Wis1
RefSeq mRNANM_001021367972h- MAP kinase kinase Wis1 (wis1), mRNA
European Nucleotide ArchiveCAA44499.1ENA Protein Mapping
European Nucleotide ArchiveCAB52609.1ENA Protein Mapping
UniParcUPI0000138F12UniProt Archive

Literature for wis1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015