wis1 (SPBC409.07c)

Gene Standard Namewis1 ChromosomeII
Systematic IDSPBC409.07c Gene Start1146633
Synonymssmf2, spc2 Gene End1143731
ProductMAP kinase kinase Wis1 Gene Length2903
Feature Typeprotein coding CDS Start1145784
Name Description CDS End1143967
Characterisation Statuspublished CDS Length1818

Ensembl Gene Location
  1. SPBC1709.19c
  2. SPNCRNA.1414
  3. mis12
  4. swi5
  5. skp1
  6. uch2
  7. wis1
  8. SPNCRNA.1415
  9. SPBC409.08
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000245loss of viability in stationary phaseCell growth assaywis1delta (deletion)deletionNot specifiedPECO:0000005, PECO:0000126PMID:1756736
FYPO:0000428abolished entry into G0Cell growth assaywis1-558 (G461D)amino_acid_mutationPECO:0000090, PECO:0000005PMID:19366728
Cell growth assaywis1-982 (F307S)amino_acid_mutationPECO:0000090, PECO:0000005PMID:19366728
FYPO:0000648small viable vegetative cellMicroscopywis1+ (wild type)wild_typeOverexpressionPMID:1756736
FYPO:0000709decreased cell population growth during nitrogen starvationCell growth assaywis1-982 (F307S)amino_acid_mutationPECO:0000005, PECO:0000095PMID:19366728
Cell growth assaywis1-558 (G461D)amino_acid_mutationPECO:0000005, PECO:0000095PMID:19366728
FYPO:0000836increased protein levelWestern blot assaywis1-982 (F307S)amino_acid_mutationPECO:0000005, PECO:0000090PMID:19366728
assayed_using cdc13
FYPO:0000998elongated cell during nitrogen starvationMicroscopywis1-982 (F307S)amino_acid_mutationPECO:0000090, PECO:0000005PMID:19366728
Microscopywis1-558 (G461D)amino_acid_mutationPECO:0000090, PECO:0000005PMID:19366728
FYPO:0000999enlarged nucleus during nitrogen starvationMicroscopywis1-982 (F307S)amino_acid_mutationEndogenousPMID:19366728
Microscopywis1-558 (G461D)amino_acid_mutationPECO:0000090, PECO:0000005PMID:19366728
FYPO:0001001abnormal cell cycle arrest in mitotic G2 phase during nitrogen starvationFlow cytometry datawis1-982 (F307S)amino_acid_mutationPECO:0000090, PECO:0000005PMID:19366728
Flow cytometry datawis1-558 (G461D)amino_acid_mutationPECO:0000090, PECO:0000005PMID:19366728
FYPO:0001228nuclear protein accumulation during cellular response to hydrogen peroxideMicroscopywis1+ (wild type)wild_typeOverexpressionPMID:9585505
assayed_using pap1
FYPO:0001266normal protein phosphorylation during cellular response to salt stressWestern blot assaywis1delta (deletion)deletionPMID:9135147
assayed_using pmk1
FYPO:0001282abnormal chromatin organization during nitrogen starvationMicroscopywis1-982 (F307S)amino_acid_mutationNot specifiedPMID:19366728
FYPO:0001420normal vegetative cell population growth rateCell growth assaywis1delta (deletion)deletionNot specifiedPECO:0000126PMID:1756736
FYPO:0001995normal cell population growth on polymyxin BCell growth assaywis1+ (wild type)wild_typeOverexpressionPMID:1756736
FYPO:0002060viable vegetative cell populationMicroscopywis1delta (deletion)deletionPMID:20473289
wis1delta (deletion)deletionPMID:15821139
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0004674protein serine/threonine kinase activityIEAUniProtKB:KW-0723GO_REF:0000037
GO:0004708MAP kinase kinase activity
has_direct_input sty1TASPMID:8649397
has_direct_input win1NASGO_REF:0000001
GO:0005515protein bindingIPIsty1PMID:9585506
GO:0005524ATP bindingISMPFAM:PF00069GO_REF:0000001
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0006468protein phosphorylationIGIsty1PMID:8886983
GO:0010389regulation of G2/M transition of mitotic cell cycleIMPPMID:1756736
GO:0036283positive regulation of transcription factor import into nucleus in response to oxidative stress
has_regulation_target pap1IMPPMID:9585505
GO:0043555regulation of translation in response to stressIMPPMID:7859738
GO:0046685response to arsenic-containing substanceIMPPMID:16087744
GO:0051403stress-activated MAPK cascadeTASPMID:15870269
GO:0051519activation of bipolar cell growthIMPPMID:20501954
GO:0070301cellular response to hydrogen peroxideIMPPMID:16087744
GO:0070314G1 to G0 transition
happens_during cellular response to nitrogen starvationIMPPMID:19366728
GO:0071470cellular response to osmotic stressTASPMID:7859738
GO:0071849G1 cell cycle arrest in response to nitrogen starvationIMPPMID:19366728
GO:1900528regulation of cell shape involved in G1 to G0 transitionIMPPMID:19366728
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005634nucleus
exists_during cellular response to stressIDAPMID:12181336
GO:0005737cytoplasmIDAPMID:9585506
GO:0005829cytosolIDAPMID:16823372
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
111466331143731

References

Region Start End Reference
five_prime_UTR11466331145785PMID:21511999
five_prime_UTR11466331145785PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00069 IPR000719 Pfam Protein kinase, catalytic domain 322 579 109
SM00220 IPR002290 SMART Serine/threonine- / dual specificity protein kinase, catalytic domain 320 579 74
PS50011 IPR000719 Prosite Profiles Protein kinase, catalytic domain 320 579 110
PS00107 IPR017441 Prosite Patterns Protein kinase, ATP binding site 326 349 73
PS00108 IPR008271 Prosite Patterns Serine/threonine-protein kinase, active site 437 449 97
G3DSA:3.30.200.20 Gene3D 315 383 108
G3DSA:1.10.510.10 Gene3D 384 586 111
SSF56112 IPR011009 SuperFamily 318 585 127
PTHR24360 hmmpanther 54 599 2
PTHR24360:SF3 hmmpanther 54 599 1

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:18257517
PMID:19547744

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0012556WIn SuppressingPMID:1756736Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0012556

Protein Properties

Ave. residue weight 107.05 Da
Charge 13.50
Isoelectric point 9.40
Molecular weight 64.76 kDa
Number of residues 605
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide7210.96PMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide4753.27PMID:23101633
RNA levelNorthern assay evidencesingle-celled organism vegetative growth phasepresentPMID:1756736
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide7.1PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide2PMID:23101633
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
atf1transcription factor, Atf-CREB family Atf1 Phenotypic SuppressionPMID:15870269
Synthetic RescuePMID:8824587
ptc3protein phosphatase 2c homolog 3 Synthetic RescuePMID:7859738
ptc1protein phosphatase 2C Ptc1 Synthetic RescuePMID:7859738
cdc25M phase inducer phosphatase Cdc25 Synthetic LethalityPMID:9843572
Phenotypic SuppressionPMID:1756736
gpd1glycerol-3-phosphate dehydrogenase Gpd1 Dosage RescuePMID:7498541
mcs4response regulator Mcs4 Dosage RescuePMID:9136929
Synthetic Rescue
pyp1tyrosine phosphatase Pyp1 Phenotypic EnhancementPMID:9321395
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:1756736
lsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Phenotypic SuppressionPMID:22144909
ppa1minor serine/threonine protein phosphatase Ppa1 Phenotypic SuppressionPMID:1756736
ppa2serine/threonine protein phosphatase Ppa2 Phenotypic SuppressionPMID:1756736
ran1serine/threonine protein kinase Ran1 Synthetic RescuePMID:8832415
win1MAP kinase kinase kinase Win1 Synthetic RescuePMID:9321395
Dosage RescuePMID:9693384
Synthetic RescuePMID:9450957
Dosage Rescue
wis4MAP kinase kinase kinase Wis4 Synthetic RescuePMID:9321395
Dosage RescuePMID:9693384
Synthetic RescuePMID:9450957
sty1MAP kinase Sty1 Phenotypic SuppressionPMID:7501024
Synthetic RescuePMID:9450957
gti1gluconate transporter inducer Gti1 Phenotypic SuppressionPMID:9372449
tup11transcriptional corepressor Tup11 Synthetic Growth DefectPMID:11238405
Phenotypic Suppression
ptc4protein phosphatase 2C Ptc4 Phenotypic EnhancementPMID:22139357
srk1MAPK-activated protein kinase Srk1 Synthetic RescuePMID:12080074
cyr1adenylate cyclase Synthetic RescuePMID:15189983
gpd2glycerol-3-phosphate dehydrogenase Gpd2 Dosage RescuePMID:7498541
tup12transcriptional corepressor Tup12 Phenotypic SuppressionPMID:11238405
Synthetic Growth Defect
sts5RNB-like protein Synthetic RescuePMID:8886983
wee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:1756736
pck2protein kinase C (PKC)-like Pck2 Phenotypic SuppressionPMID:9199286
mkh1MEK kinase (MEKK) Mkh1 Phenotypic SuppressionPMID:9199286
Phenotypic Enhancement
cmk2MAPK-activated protein kinase Cmk2 Synthetic RescuePMID:11886858
isp6vacuolar serine protease Isp6 Phenotypic EnhancementPMID:16550352
leu13-isopropylmalate dehydrogenase Leu1 Phenotypic SuppressionPMID:15466417
cut1separase/separin Synthetic RescuePMID:16861909
cdc37Hsp90 co-chaperone Cdc37 Synthetic RescuePMID:12861001
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
win1MAP kinase kinase kinase Win1 Biochemical ActivityPMID:9693384
sty1MAP kinase Sty1 Biochemical ActivityPMID:10398679
Biochemical ActivityPMID:9718372
Biochemical ActivityPMID:7501024
Affinity Capture-WesternPMID:12181336
wis4MAP kinase kinase kinase Wis4 Biochemical ActivityPMID:9321395
Biochemical ActivityPMID:9718372
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External References
Database Identifier Description
NBRP SPBC409.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC409.07c Retrieval of eukaryotic orthologs
BioGrid SPBC409.07c BioGRID Interaction Datasets
Bähler Lab SPBC409.07c Cell Cycle Expression Profile
Bähler Lab SPBC409.07c Meiosis/Sporulation Expression Profies
Bähler Lab SPBC409.07c Pheromone response/mating expression profiles
Bähler Lab SPBC409.07c Environmental stress expression profiles
Bähler Lab SPBC409.07c Bähler Lab Transcriptome Viewer
Cyclebase SPBC409.07c Cell Cycle Data
PInt SPBC409.07c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPBC409.07c Entrez Gene
WikiGene2541055MAP kinase kinase Wis1
Uniprot_genenameWIS1
EntrezGene2541055MAP kinase kinase Wis1
SPD / RIKEN33/33D12Orfeome Localization Data
UniProtKB/SwissProtP33886Protein kinase wis1
ModBaseP33886Database of comparative protein structure models
Pfam Protein DomainsP33886Pfam Domain Arrangement
RefSeq PeptideNP_595457MAP kinase kinase Wis1
RefSeq mRNANM_001021367972h- MAP kinase kinase Wis1 (wis1), mRNA
European Nucleotide ArchiveCAA44499ENA Protein Mapping
European Nucleotide ArchiveCAB52609ENA Protein Mapping
UniParcUPI0000138F12UniProt Archive

Literature for wis1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013