wis1 (SPBC409.07c)


Gene Standard Namewis1 Characterisation Statuspublished
Systematic IDSPBC409.07c Feature Typeprotein coding
Synonymssmf2, spc2 Name DescriptionWIn Suppressing
ProductMAP kinase kinase Wis1 Product Size605aa, 64.76 kDa
Genomic Location Chromosome II, 1146633-1143731 (2903nt); CDS:1145784-1143967 (1818nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0004708MAP kinase kinase activity3
has substrate sty1IDAPMID:9718372
has substrate sty1IDAPMID:7501024
GO:0004715non-membrane spanning protein tyrosine kinase activity1
has substrate sty1IDAPMID:7501024
GO:0005515protein bindingIPIsty1PMID:9585506831
GO:0004674protein serine/threonine kinase activityIEAIPR008271GO_REF:0000002112
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0071849G1 cell cycle arrest in response to nitrogen starvationIMPPMID:193667286
GO:0070314G1 to G0 transition9
during cellular response to nitrogen starvationIMPPMID:19366728
GO:0000165MAPK cascadeIDAPMID:971837222
GO:1990263MAPK cascade in response to starvationIMPPMID:75010242
GO:0000161MAPK cascade involved in osmosensory signaling pathwayIMPPMID:75010243
GO:0000187activation of MAPK activityIDAPMID:97183723
GO:0030996cell cycle arrest in response to nitrogen starvationIMPPMID:882458710
GO:0018108peptidyl-tyrosine phosphorylationIDAPMID:750102411
GO:0010971positive regulation of G2/M transition of mitotic cell cycleIMPPMID:750102410
GO:0036283positive regulation of transcription factor import into nucleus in response to oxidative stress2
regulates pap1IMPPMID:9585505
GO:0046777protein autophosphorylationIMPPMID:969338414
GO:0006468protein phosphorylationIMPPMID:9718372105
GO:0010389regulation of G2/M transition of mitotic cell cycleIMPPMID:175673663
GO:1900528regulation of cell shape involved in G1 to G0 transitionIMPPMID:193667282
GO:0043555regulation of translation in response to stressIMPPMID:785973810
GO:0051403stress-activated MAPK cascadeIMPPMID:97183728
IMPPMID:8649397
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1990315Mcs4 RR-MAPKKK complex3
except during cellular response to osmotic stressIDAPMID:24255738
except during cellular response to hydrogen peroxideIDAPMID:24255738
GO:0005737cytoplasmIDAPMID:95855064205
GO:0005829cytosolIDAPMID:168233722315
GO:0005634nucleus2740
during cellular response to stressIDAPMID:12181336
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003535decreased bipolar indexMicroscopywis1ΔNullPECO:0000004PMID:2050195427
Microscopywis1ΔNullPECO:0000005PMID:20501954
FYPO:0000082decreased cell population growth at high temperatureCell growth assayK349R (K349R)OverexpressionPECO:0000126, PECO:0000102PMID:9718372148
Cell growth assayAA (S469A, T473A)EndogenousPECO:0000201, PECO:0000102PMID:9718372
Cell growth assayAAA (S469A, S471A, T473A)EndogenousPECO:0000102, PECO:0000201PMID:9718372
FYPO:0000709decreased cell population growth during nitrogen starvationCell growth assaywis1-558 (G461D)PECO:0000095, PECO:0000005PMID:1936672835
Cell growth assaywis1-982 (F307S)PECO:0000095, PECO:0000005PMID:19366728
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaywis1ΔNullPECO:0000126, PECO:0000005, PECO:0000103PMID:8557102147
FYPO:0001128decreased septation indexMicroscopywis1ΔNullPECO:0000004PMID:2050195419
FYPO:0003532increased monopolar indexMicroscopywis1ΔNullPECO:0000005PMID:2050195435
Microscopywis1ΔNullPECO:0000004PMID:20501954
FYPO:0000245loss of viability in stationary phaseCell growth assaywis1ΔNullPECO:0000005, PECO:0000126PMID:175673641
Cell growth assaywis1ΔNullPECO:0000126, PECO:0000005PMID:8557102
FYPO:0000674normal cell population growth at high temperatureCell growth assayAAA (S469A, S471A, T473A)OverexpressionPECO:0000102, PECO:0000126PMID:971837237
FYPO:0001164normal growth on glucose carbon sourceCell growth assaywis1ΔNullPECO:0000137, PECO:0000005, PECO:0000102PMID:22912829176
FYPO:0001420normal vegetative cell population growth rateCell growth assaywis1ΔNullPECO:0000126, PECO:0000005, PECO:0000103PMID:855710251
Cell growth assayAA (S469A, T473A)EndogenousPECO:0000005, PECO:0000102PMID:9718372
Cell growth assayAAA (S469A, S471A, T473A)EndogenousPECO:0000005, PECO:0000102PMID:9718372
Cell growth assaywis1ΔNullPECO:0000126PMID:1756736
FYPO:0000073resistance to caffeineCell growth assaywis1DDPECO:0000137PMID:1967230621
FYPO:0002634resistance to cobalt56
expressivity FYPO_EXT:0000002Cell growth assaywis1ΔNullPECO:0000201, PECO:0000005PMID:22806344
FYPO:0001103resistance to hydrogen peroxideCell growth assaywis1DDPECO:0000137PMID:1967230667
FYPO:0000268sensitive to UV during vegetative growthCell growth assaywis1ΔNullPECO:0000126, PECO:0000005PMID:8557102123
FYPO:0002806sensitive to arsenateCell growth assaywis1ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:229128292
FYPO:0000095sensitive to bleomycinCell growth assaywis1ΔNullPECO:0000126, PECO:0000005PMID:855710248
FYPO:0000097sensitive to caffeine105
expressivity FYPO_EXT:0000001Cell growth assaywis1ΔNullPECO:0000137PMID:19672306
FYPO:0001408sensitive to heat shockCell growth assaywis1ΔNullPECO:0000137PMID:86493976
Cell growth assaywis1ΔNullPECO:0000126, PECO:0000005PMID:8557102
FYPO:0000081sensitive to high osmolarityCell growth assaywis1ΔNullPECO:0000166, PECO:0000005, PECO:0000126PMID:855710211
Cell growth assayAAA (S469A, S471A, T473A)EndogenousPECO:0000005, PECO:0000102, PECO:0000201PMID:9718372
Cell growth assayAA (S469A, T473A)EndogenousPECO:0000005, PECO:0000102, PECO:0000201PMID:9718372
FYPO:0000087sensitive to hydrogen peroxide131
expressivity FYPO_EXT:0000001Cell growth assaywis1ΔNullPECO:0000137PMID:19672306
Cell growth assaywis1ΔNullPECO:0000137PMID:8649397
FYPO:0000267sensitive to ionizing radiationCell growth assaywis1ΔNullPECO:0000126, PECO:0000005PMID:855710246
FYPO:0002811sensitive to non-ionic osmotic stressCell growth assaywis1ΔNullPECO:0000102, PECO:0000126, PECO:0000025PMID:74985418
FYPO:0000271sensitive to salt stressCell growth assaywis1ΔNullPECO:0000126, PECO:0000005, PECO:0000166PMID:855710284
Cell growth assaywis1ΔNullPECO:0000102, PECO:0000005, PECO:0000137, PECO:0000207PMID:9135083
Cell growth assaywis1ΔNullPECO:0000102, PECO:0000126, PECO:0000166PMID:7498541
Cell growth assaywis1ΔNullPECO:0000102, PECO:0000207, PECO:0000201PMID:10526174
FYPO:0000112sensitive to sorbitolCell growth assaywis1ΔNullPECO:0000102, PECO:0000201PMID:105261747
Cell growth assayAA (S469A, T473A)EndogenousPECO:0000201, PECO:0000102, PECO:0000005PMID:9718372
Cell growth assayAAA (S469A, S471A, T473A)EndogenousPECO:0000201, PECO:0000005, PECO:0000102PMID:9718372
FYPO:0000086sensitive to tacrolimus81
expressivity FYPO_EXT:0000002Cell growth assaywis1ΔNullPECO:0000200, PECO:0000005, PECO:0000142PMID:21850271
FYPO:0002060viable vegetative cell populationMicroscopywis1ΔNullPMID:158211393759
Microscopywis1ΔNullPMID:20473289
Microscopywis1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001996RNA absent from cell during nitrogen starvation12
affecting ste11Transcript expression level evidencewis1ΔNullPECO:0000127, PECO:0000104PMID:9671458
FYPO:0001001abnormal cell cycle arrest in mitotic G2 phase during nitrogen starvationFlow cytometry datawis1-558 (G461D)PECO:0000005, PECO:0000090PMID:193667289
Flow cytometry datawis1-982 (F307S)PECO:0000005, PECO:0000090PMID:19366728
FYPO:0000710abnormal cell cycle arrest in response to nitrogen starvation20
penetrance FYPO_EXT:0000001Flow cytometry datawis1ΔNullPMID:9135083
FYPO:0001282abnormal chromatin organization during nitrogen starvationMicroscopywis1-982 (F307S)Not specifiedPMID:193667283
FYPO:0003625abnormal microtubule cytoskeleton morphology during mitotic interphase186
penetrance FYPO_EXT:0000002experimental phenotypic evidencewis1ΔNullPECO:0000005, PECO:0000103, PECO:0000137DOI:10.1016/j.devcel.2014.09.005
FYPO:0000059abnormal mitotic cell cycleMicroscopywis1ΔNullPECO:0000005, PECO:0000137PMID:23697806686
FYPO:0000428abolished entry into G0Cell growth assaywis1-982 (F307S)PECO:0000005, PECO:0000090PMID:193667285
Cell growth assaywis1-558 (G461D)PECO:0000005, PECO:0000090PMID:19366728
FYPO:0003020abolished protein autophosphorylation during vegetative growth5
affecting wis1Western blot assayK349R (K349R)Not specifiedPMID:9693384
FYPO:0001384abolished protein kinase activity15
affecting sty1Enzyme assay datawis1ΔNullPMID:7501024
affecting wis1Western blot assayK349R (K349R)Not specifiedPECO:0000140PMID:9718372
FYPO:0003477abolished protein phosphorylation during cellular response to arsenic-containing substance1
affecting sty1Western blot assaywis1ΔNullPECO:0000258PMID:22912829
FYPO:0002033abolished protein phosphorylation during vegetative growth23
affecting sty1Western blot assayK349R (K349R)Not specifiedPMID:9693384
FYPO:0002288abolished protein tyrosine phosphorylation4
affecting sty1Western blot assaywis1ΔNullPMID:7501024
affecting sty1Western blot assaywis1ΔNullPMID:9693384
FYPO:0002283abolished protein tyrosine phosphorylation during cellular response to heat1
affecting sty1Western blot assayAAA (S469A, S471A, T473A)EndogenousPMID:9718372
affecting sty1Western blot assaywis1ΔNullPMID:8649397
FYPO:0002284abolished protein tyrosine phosphorylation during cellular response to hydrogen peroxide1
affecting sty1Western blot assaywis1ΔNullPMID:8649397
FYPO:0002289abolished protein tyrosine phosphorylation during cellular response to osmotic stress1
affecting sty1Western blot assayAAA (S469A, S471A, T473A)EndogenousPMID:9718372
FYPO:0002663alpha,alpha-trehalase activity increase abolished during cellular response to hydrogen peroxideEnzyme assay datawis1ΔNullPMID:99742194
FYPO:0003034decreased RNA level during cellular response to UV3
affecting uvi15Transcript expression level evidencewis1ΔNullPMID:10954610
FYPO:0002623decreased RNA level during cellular response to glucose stimulus4
affecting mae2Transcript expression level evidencewis1 mutantNot specifiedPMID:11453251
FYPO:0002287decreased RNA level during cellular response to heat4
affecting gpd1Transcript expression level evidenceK349R (K349R)OverexpressionPMID:9718372
affecting ctt1Transcript expression level evidenceK349R (K349R)OverexpressionPMID:9718372
affecting pyp2Transcript expression level evidenceAAA (S469A, S471A, T473A)OverexpressionPMID:9718372
affecting gpd1Transcript expression level evidenceAAA (S469A, S471A, T473A)OverexpressionPMID:9718372
affecting pyp2Transcript expression level evidenceK349R (K349R)OverexpressionPMID:9718372
affecting ctt1Transcript expression level evidenceAAA (S469A, S471A, T473A)OverexpressionPMID:9718372
FYPO:0001116decreased RNA level during cellular response to hydrogen peroxide13
affecting ctt1Transcript expression level evidenceK349R (K349R)Not specifiedPMID:9718372
affecting gpd1Transcript expression level evidenceAAA (S469A, S471A, T473A)OverexpressionPMID:9718372
affecting pyp2Transcript expression level evidenceAAA (S469A, S471A, T473A)OverexpressionPMID:9718372
affecting pyp2Transcript expression level evidenceK349R (K349R)Not specifiedPMID:9718372
affecting gpd1Transcript expression level evidenceK349R (K349R)Not specifiedPMID:9718372
affecting ctt1Transcript expression level evidenceAAA (S469A, S471A, T473A)OverexpressionPMID:9718372
affecting ntp1Transcript expression level evidencewis1ΔNullPMID:9974219
FYPO:0001259decreased RNA level during cellular response to hypoxia5
affecting mae2Transcript expression level evidencewis1 mutantNot specifiedPMID:11453251
FYPO:0002305decreased RNA level during cellular response to non-ionic osmotic stress3
affecting cta3Transcript expression level evidencewis1ΔNullPECO:0000025, PECO:0000201PMID:10428498
FYPO:0002304decreased RNA level during cellular response to salt stress8
affecting cta3Transcript expression level evidencewis1ΔNullPECO:0000166, PECO:0000201PMID:10428498
affecting gpd1Transcript expression level evidencewis1ΔNullPECO:0000201, PECO:0000166PMID:7498541
FYPO:0001152decreased RNA level during nitrogen starvation28
affecting ste11Transcript expression level evidencewis1ΔNullPMID:9135083
affecting ste11Transcript expression level evidencewis1ΔNullPMID:8824587
FYPO:0001117decreased RNA level during vegetative growth111
affecting gpd1Transcript expression level evidencewis1ΔNullPECO:0000201PMID:7498541
FYPO:0000711decreased cell cycle arrest in mitotic G1 phase in response to nitrogen starvationFlow cytometry datawis1ΔNullPMID:88245876
FYPO:0002813decreased cellular glycerol level during cellular response to salt stressSubstance quantification evidencewis1ΔNullPECO:0000166PMID:74985412
FYPO:0000708decreased mating efficiencyMicroscopywis1ΔNullPMID:9718372240
Cell growth assaywis1-DD (S469D, T473D)Not specifiedPMID:16550352
Cell growth assaywis1ΔNullPMID:8557102
MicroscopyAA (S469A, T473A)EndogenousPMID:9718372
MicroscopyAAA (S469A, S471A, T473A)EndogenousPMID:9718372
FYPO:0002679decreased protein phosphorylation45
affecting wis1Western blot assayAA (S469A, T473A)Not specifiedPECO:0000140PMID:9718372
affecting wis1Western blot assayAAA (S469A, S471A, T473A)Not specifiedPECO:0000140PMID:9718372
FYPO:0001838decreased protein phosphorylation during vegetative growth43
affecting atf1Gel electrophoresis evidencewis1ΔNullPMID:9135083
FYPO:0003140decreased protein tyrosine phosphorylation during cellular response to heat1
affecting sty1Western blot assayAAA (S469A, S471A, T473A)OverexpressionPMID:9718372
FYPO:0003141decreased protein tyrosine phosphorylation during cellular response to hydrogen peroxide1
affecting sty1Western blot assayAAA (S469A, S471A, T473A)OverexpressionPMID:9718372
FYPO:0000017elongated cellMicroscopywis1-558 (G461D)PECO:0000004PMID:19366728696
FYPO:0000998elongated cell during nitrogen starvationMicroscopywis1-982 (F307S)PECO:0000005, PECO:0000090PMID:1936672818
Microscopywis1-558 (G461D)PECO:0000005, PECO:0000090PMID:19366728
FYPO:0000999enlarged nucleus during nitrogen starvationMicroscopywis1-982 (F307S)EndogenousPMID:193667282
Microscopywis1-558 (G461D)PECO:0000005, PECO:0000090PMID:19366728
FYPO:0000825increased RNA level during vegetative growth148
affecting pyp2Transcript expression level evidencewis1+ (wild type)OverexpressionPMID:8649397
FYPO:0001228increased nuclear protein level during cellular response to hydrogen peroxide2
affecting pap1Microscopywis1+ (wild type)OverexpressionPMID:9585505
FYPO:0000836increased protein level72
affecting cdc13Western blot assaywis1-982 (F307S)PECO:0000090, PECO:0000005PMID:19366728
FYPO:0003075normal protein kinase activity8
affecting wis1Enzyme assay dataAAA (S469A, S471A, T473A)Not specifiedPMID:9718372
FYPO:0000833normal protein level40
affecting wis1Western blot assayAAA (S469A, S471A, T473A)EndogenousPMID:9718372
affecting wis1Western blot assayAA (S469A, T473A)EndogenousPMID:9718372
FYPO:0001266normal protein phosphorylation during cellular response to salt stress4
affecting pmk1Western blot assaywis1ΔNullPMID:9135147
FYPO:0000280sterileMicroscopywis1ΔNullPECO:0000127, PECO:0000104PMID:967145850
FYPO:0000646swollen vegetative cellMicroscopywis1+ (wild type)OverexpressionPMID:750102446
FYPO:0000647vegetative cell lysisMicroscopywis1+ (wild type)OverexpressionPMID:750102480
FYPO:0001492viable elongated vegetative cellMicroscopywis1ΔNullPECO:0000201PMID:8943330221
MicroscopyAA (S469A, T473A)EndogenousPECO:0000126PMID:9718372
Cell growth assaywis1ΔNullPECO:0000005PMID:1756736
MicroscopyAAA (S469A, S471A, T473A)EndogenousPECO:0000126PMID:9718372
Microscopywis1ΔNullPECO:0000126PMID:9718372
expressivity FYPO_EXT:0000001
penetrance FYPO_EXT:0000001
Microscopywis1ΔNullPECO:0000137, PECO:0000005PMID:23697806
MicroscopyK349R (K349R)OverexpressionPMID:9718372
FYPO:0000648viable small vegetative cellMicroscopywis1+ (wild type)OverexpressionPMID:175673634
FYPO:0002197viable vegetative cell with abnormal cell shape467
penetrance FYPO_EXT:0000002experimental phenotypic evidencewis1ΔNullPECO:0000005, PECO:0000103, PECO:0000137DOI:10.1016/j.devcel.2014.09.005
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopyAAA (S469A, S471A, T473A)EndogenousPMID:97183723089
MicroscopyAA (S469A, T473A)EndogenousPMID:9718372
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in mcs4 response regulator Mcs4 PMID:23389634
FYPO affected by mutation in win1 MAP kinase kinase kinase Win1 PMID:23389634
FYPO affected by mutation in win1 MAP kinase kinase kinase Win1 PMID:9693384
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:9693384
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:9718372
GO regulated by win1 MAP kinase kinase kinase Win1 PMID:9693384
GO substrate of win1 MAP kinase kinase kinase Win1 PMID:9693384
GO substrate of wis4 MAP kinase kinase kinase Wis4 PMID:9718372
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
111466331143731

UTRs

Region Coordinates Reference
five_prime_UTR1146633..1145785PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 322 579 109
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 320 579 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 320 579 110
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 437 449 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 326 349 73
PTHR24360:SF3 HMMPANTHER 78 600 1
PTHR24360 HMMPANTHER 78 600 3
3.30.200.20 Gene3D 315 383 111
1.10.510.10 Gene3D 384 586 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 318 585 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.05 Da
Charge 13.50
Isoelectric point 9.40
Molecular weight 64.76 kDa
Number of residues 605
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDA469PMID:97183721670
added by wis4
present during mitotic M phaseexperimental evidenceS129PMID:21712547
present during mitotic M phaseexperimental evidenceS197PMID:21712547
experimental evidenceS129PMID:24763107
present during cellular response to thiabendazoleIDAS129PMID:18257517
present during mitotic M phaseexperimental evidenceS201PMID:21712547
MOD:00047O-phospho-L-threonineIDA473PMID:9718372692
added by wis4
present during mitotic M phaseexperimental evidenceT132PMID:21712547
experimental evidenceT132PMID:24763107
MOD:00696phosphorylated residueNASPMID:195477441922
NASPMID:18257517
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:1756736

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
10153during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
12460during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
12150during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
12134during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4753.27during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
12418during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7210.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
nrd1RNA-binding protein Nrd1 Phenotypic SuppressionPMID:9671458
tup11transcriptional corepressor Tup11 Synthetic Growth DefectPMID:11238405
Phenotypic Suppression
ptc1protein phosphatase 2C Ptc1 Synthetic RescuePMID:7859738
leu13-isopropylmalate dehydrogenase Leu1 Phenotypic SuppressionPMID:15466417
pyp1tyrosine phosphatase Pyp1 Phenotypic EnhancementPMID:9321395
sds23PP2A-type phosphatase inhibitor Sds23/Moc1 Dosage LethalityPMID:23640764
Phenotypic Enhancement
lsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Phenotypic SuppressionPMID:22144909
cmk2MAPK-activated protein kinase Cmk2 Synthetic RescuePMID:11886858
ptc4protein phosphatase 2C Ptc4 Phenotypic EnhancementPMID:22139357
cdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic SuppressionPMID:1756736
Dosage Rescue
Synthetic LethalityPMID:9843572
Phenotypic SuppressionPMID:8557102
cut1separase/separin Synthetic RescuePMID:16861909
srk1MAPK-activated protein kinase Srk1 Synthetic RescuePMID:12080074
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:1756736
sty1MAP kinase Sty1 Synthetic RescuePMID:9450957
Phenotypic SuppressionPMID:7501024
Phenotypic SuppressionPMID:8649397
Dosage RescuePMID:8557102
wis4MAP kinase kinase kinase Wis4 Synthetic RescuePMID:9450957
Synthetic RescuePMID:9718372
Synthetic RescuePMID:9321395
Dosage RescuePMID:9693384
Phenotypic Suppression
tup12transcriptional corepressor Tup12 Synthetic Growth DefectPMID:11238405
Phenotypic Suppression
ppa1minor serine/threonine protein phosphatase Ppa1 Phenotypic SuppressionPMID:1756736
ptc3protein phosphatase 2c homolog 3 Synthetic RescuePMID:7859738
wee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:1756736
Dosage Rescue
cyr1adenylate cyclase Synthetic RescuePMID:15189983
gti1gluconate transporter inducer Gti1 Phenotypic SuppressionPMID:9372449
mcs4response regulator Mcs4 Synthetic RescuePMID:9136929
Dosage Rescue
atf1transcription factor, Atf-CREB family Atf1 Synthetic RescuePMID:8824587
Phenotypic SuppressionPMID:15870269
pka1cAMP-dependent protein kinase catalytic subunit Pka1 Synthetic Growth DefectPMID:23640764
gpd2glycerol-3-phosphate dehydrogenase Gpd2 Dosage RescuePMID:7498541
win1MAP kinase kinase kinase Win1 Dosage RescuePMID:1756736
Dosage RescuePMID:9450957
Synthetic Rescue
Synthetic RescuePMID:9321395
Phenotypic SuppressionPMID:9693384
Dosage Rescue
sts5RNB-like protein Synthetic RescuePMID:8886983
pck2protein kinase C (PKC)-like Pck2 Phenotypic SuppressionPMID:9199286
gpd1glycerol-3-phosphate dehydrogenase Gpd1 Dosage RescuePMID:7498541
cdc37Hsp90 co-chaperone Cdc37 Synthetic RescuePMID:12861001
isp6vacuolar serine protease Isp6 Phenotypic EnhancementPMID:16550352
ran1serine/threonine protein kinase Ran1 Synthetic RescuePMID:8832415
mkh1MEK kinase (MEKK) Mkh1 Phenotypic SuppressionPMID:9199286
Phenotypic Enhancement
byr1MAP kinase kinase Byr1 Synthetic Growth DefectPMID:8557102
ppa2serine/threonine protein phosphatase Ppa2 Phenotypic SuppressionPMID:1756736
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
wis1MAP kinase kinase Wis1 Biochemical ActivityPMID:9718372
mpr1histidine-containing response regulator phosphotransferase Mpr1 Two-hybridPMID:23695164
sty1MAP kinase Sty1 Affinity Capture-WesternPMID:12181336
Biochemical ActivityPMID:9718372
Biochemical ActivityPMID:7501024
Biochemical ActivityPMID:10398679
Two-hybridPMID:23695164
win1MAP kinase kinase kinase Win1 Affinity Capture-WesternPMID:24255738
Biochemical ActivityPMID:9693384
Affinity Capture-LuminescencePMID:23389634
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
wis4MAP kinase kinase kinase Wis4 Biochemical ActivityPMID:9718372
Biochemical ActivityPMID:9321395
Affinity Capture-LuminescencePMID:23389634
External References
Database Identifier Description
NBRP SPBC409.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC409.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC409.07c BioGRID Interaction Datasets
Expression Viewer SPBC409.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC409.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC409.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC409.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC409.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC409.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC409.07c Cell Cycle Data
GEO SPBC409.07c GEO profiles
PInt SPBC409.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC409.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC409.07c Fission yeast phenotypic data & analysis
SPD / RIKEN33/33D12Orfeome Localization Data
PUBMED9135147
UniProtKB/SwissProtP33886Protein kinase wis1
ModBaseP33886Database of comparative protein structure models
STRINGP33886Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595457MAP kinase kinase Wis1
RefSeq mRNANM_001021367972h- MAP kinase kinase Wis1 (wis1), mRNA
European Nucleotide ArchiveCAA44499.1ENA Protein Mapping
European Nucleotide ArchiveCAB52609.1ENA Protein Mapping
UniParcUPI0000138F12UniProt Archive

Literature for wis1

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Release Version: PomBase:23_47 - 27 Oct 2014