wis1 (SPBC409.07c)


Gene Standard Namewis1 Characterisation Statuspublished
Systematic IDSPBC409.07c Feature Typeprotein coding
Synonymssmf2, spc2 Name Description
ProductMAP kinase kinase Wis1 Product Size605aa, 64.76 kDa
Genomic Location Chromosome II, 1146633-1143731 (2903nt); CDS:1145784-1143967 (1818nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0004708MAP kinase kinase activity3
has substrate win1NASGO_REF:0000001
has substrate sty1IDAPMID:7501024
GO:0004715non-membrane spanning protein tyrosine kinase activity2
has substrate sty1IDAPMID:7501024
GO:0005515protein bindingIPIsty1PMID:9585506887
GO:0004674protein serine/threonine kinase activityIEAUniProtKB-KW:KW-0723GO_REF:0000037112
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0071849G1 cell cycle arrest in response to nitrogen starvationIMPPMID:193667284
GO:0070314G1 to G0 transition9
during cellular response to nitrogen starvationIMPPMID:19366728
GO:1990263MAPK cascade in response to starvationIMPPMID:75010242
GO:0000161MAPK cascade involved in osmosensory signaling pathwayIMPPMID:75010243
GO:0000187activation of MAPK activityNASGO_REF:00000013
GO:0051519activation of bipolar cell growthIMPPMID:2050195442
GO:0070301cellular response to hydrogen peroxideIMPPMID:1608774414
GO:0018108peptidyl-tyrosine phosphorylationIDAPMID:75010243
GO:0010971positive regulation of G2/M transition of mitotic cell cycleIMPPMID:75010249
GO:0036283positive regulation of transcription factor import into nucleus in response to oxidative stress2
regulates pap1IMPPMID:9585505
GO:0046777protein autophosphorylationIMPPMID:96933842
GO:0006468protein phosphorylationIGIsty1PMID:8886983101
GO:0010389regulation of G2/M transition of mitotic cell cycleIMPPMID:175673667
GO:1900528regulation of cell shape involved in G1 to G0 transitionIMPPMID:193667282
GO:0043555regulation of translation in response to stressIMPPMID:785973810
GO:0046685response to arsenic-containing substanceIMPPMID:160877445
GO:0051403stress-activated MAPK cascadeIMPPMID:86493975
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:95855064198
GO:0005829cytosolIDAPMID:168233722317
GO:0005634nucleus2730
during cellular response to stressIDAPMID:12181336
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000709decreased cell population growth during nitrogen starvationCell growth assaywis1-558 (G461D)PECO:0000095, PECO:0000005PMID:1936672833
Cell growth assaywis1-982 (F307S)PECO:0000095, PECO:0000005PMID:19366728
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaywis1ΔNullPECO:0000126, PECO:0000005, PECO:0000103PMID:855710285
FYPO:0000245loss of viability in stationary phaseCell growth assaywis1ΔNullPECO:0000005, PECO:0000126PMID:175673618
Cell growth assaywis1ΔNullPECO:0000126, PECO:0000005PMID:8557102
FYPO:0001995normal cell population growth on polymyxin BCell growth assaywis1+ (wild type)OverexpressionPMID:17567361
FYPO:0001420normal vegetative cell population growth rateCell growth assaywis1ΔNullPECO:0000126, PECO:0000005, PECO:0000103PMID:855710229
Cell growth assaywis1ΔNullPECO:0000126PMID:1756736
FYPO:0000073resistance to caffeineCell growth assaywis1DDPECO:0000137PMID:1967230618
FYPO:0002634resistance to cobaltCell growth assaywis1ΔNullPECO:0000201, PECO:0000005PMID:2280634456
expressivity FYPO_EXT:0000002
FYPO:0001103resistance to hydrogen peroxideCell growth assaywis1DDPECO:0000137PMID:1967230659
FYPO:0000268sensitive to UV during vegetative growthCell growth assaywis1ΔNullPECO:0000126, PECO:0000005PMID:855710282
FYPO:0000095sensitive to bleomycinCell growth assaywis1ΔNullPECO:0000126, PECO:0000005PMID:85571029
FYPO:0000097sensitive to caffeineCell growth assaywis1ΔNullPECO:0000137PMID:1967230695
expressivity FYPO_EXT:0000001
FYPO:0001408sensitive to heat shockCell growth assaywis1ΔNullPECO:0000137PMID:86493974
Cell growth assaywis1ΔNullPECO:0000126, PECO:0000005PMID:8557102
FYPO:0000081sensitive to high osmolarityCell growth assaywis1ΔNullPECO:0000166, PECO:0000005, PECO:0000126PMID:85571026
FYPO:0000087sensitive to hydrogen peroxideCell growth assaywis1ΔNullPECO:0000137PMID:19672306112
expressivity FYPO_EXT:0000001
Cell growth assaywis1ΔNullPECO:0000137PMID:8649397
FYPO:0000267sensitive to ionizing radiationCell growth assaywis1ΔNullPECO:0000126, PECO:0000005PMID:855710241
FYPO:0002811sensitive to non-ionic osmotic stressCell growth assaywis1ΔNullPECO:0000102, PECO:0000126, PECO:0000025PMID:74985416
FYPO:0000271sensitive to salt stressCell growth assaywis1ΔNullPECO:0000126, PECO:0000005, PECO:0000166PMID:855710273
Cell growth assaywis1ΔNullPECO:0000102, PECO:0000126, PECO:0000166PMID:7498541
FYPO:0000086sensitive to tacrolimusCell growth assaywis1ΔNullPECO:0000200, PECO:0000005, PECO:0000142PMID:2185027179
expressivity FYPO_EXT:0000002
FYPO:0002060viable vegetative cell populationMicroscopywis1ΔNullPECO:0000005, PECO:0000137PMID:236978063755
wis1ΔNullPMID:15821139
Microscopywis1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001996RNA absent from cell during nitrogen starvationTranscript expression level evidencewis1ΔNullPECO:0000127, PECO:0000104PMID:96714584
affecting ste11
FYPO:0001001abnormal cell cycle arrest in mitotic G2 phase during nitrogen starvationFlow cytometry datawis1-982 (F307S)PECO:0000005, PECO:0000090PMID:193667288
Flow cytometry datawis1-558 (G461D)PECO:0000005, PECO:0000090PMID:19366728
FYPO:0001282abnormal chromatin organization during nitrogen starvationMicroscopywis1-982 (F307S)Not specifiedPMID:193667283
FYPO:0000059abnormal mitotic cell cycleMicroscopywis1ΔNullPECO:0000005, PECO:0000137PMID:23697806564
FYPO:0000428abolished entry into G0Cell growth assaywis1-558 (G461D)PECO:0000005, PECO:0000090PMID:193667285
Cell growth assaywis1-982 (F307S)PECO:0000005, PECO:0000090PMID:19366728
FYPO:0003020abolished protein autophosphorylation during vegetative growthWestern blot assayK349R (K349R)Not specifiedPMID:96933841
affecting wis1
FYPO:0001384abolished protein kinase activityEnzyme assay datawis1ΔNullPMID:75010249
affecting sty1
FYPO:0002033abolished protein phosphorylation during vegetative growthWestern blot assayK349R (K349R)Not specifiedPMID:96933848
affecting sty1
FYPO:0002288abolished protein tyrosine phosphorylationWestern blot assaywis1ΔNullPMID:96933842
affecting sty1
affecting sty1Western blot assaywis1ΔNullPMID:7501024
FYPO:0002283abolished protein tyrosine phosphorylation during cellular response to heatWestern blot assaywis1ΔNullPMID:86493971
affecting sty1
FYPO:0002284abolished protein tyrosine phosphorylation during cellular response to hydrogen peroxideWestern blot assaywis1ΔNullPMID:86493971
affecting sty1
FYPO:0002663alpha,alpha-trehalase activity increase abolished during cellular response to hydrogen peroxideEnzyme assay datawis1ΔNullPMID:99742194
FYPO:0003034decreased RNA level during cellular response to UVTranscript expression level evidencewis1ΔNullPMID:109546103
affecting uvi15
FYPO:0002623decreased RNA level during cellular response to glucose stimulusTranscript expression level evidencewis1 mutantNot specifiedPMID:114532514
affecting mae2
FYPO:0001116decreased RNA level during cellular response to hydrogen peroxideTranscript expression level evidencewis1ΔNullPMID:99742195
affecting ntp1
FYPO:0001259decreased RNA level during cellular response to hypoxiaTranscript expression level evidencewis1 mutantNot specifiedPMID:114532515
affecting mae2
FYPO:0002305decreased RNA level during cellular response to non-ionic osmotic stressTranscript expression level evidencewis1ΔNullPECO:0000025, PECO:0000201PMID:104284983
affecting cta3
FYPO:0002304decreased RNA level during cellular response to salt stressTranscript expression level evidencewis1ΔNullPECO:0000166, PECO:0000201PMID:104284983
affecting cta3
affecting gpd1Transcript expression level evidencewis1ΔNullPECO:0000201, PECO:0000166PMID:7498541
FYPO:0001117decreased RNA level during vegetative growthTranscript expression level evidencewis1ΔNullPECO:0000201PMID:749854148
affecting gpd1
FYPO:0002813decreased cellular glycerol level during cellular response to salt stressSubstance quantification evidencewis1ΔNullPECO:0000166PMID:74985412
FYPO:0000708decreased mating efficiencyCell growth assaywis1-DD (S469D,T473D)Not specifiedPMID:16550352222
Cell growth assaywis1ΔNullPMID:8557102
FYPO:0000017elongated cellMicroscopywis1-558 (G461D)PECO:0000004PMID:19366728408
FYPO:0000998elongated cell during nitrogen starvationMicroscopywis1-982 (F307S)PECO:0000005, PECO:0000090PMID:1936672814
Microscopywis1-558 (G461D)PECO:0000005, PECO:0000090PMID:19366728
FYPO:0000999enlarged nucleus during nitrogen starvationMicroscopywis1-982 (F307S)EndogenousPMID:193667282
Microscopywis1-558 (G461D)PECO:0000005, PECO:0000090PMID:19366728
FYPO:0000825increased RNA level during vegetative growthTranscript expression level evidencewis1+ (wild type)OverexpressionPMID:864939781
affecting pyp2
FYPO:0001228increased nuclear protein level during cellular response to hydrogen peroxideMicroscopywis1+ (wild type)OverexpressionPMID:95855052
affecting pap1
FYPO:0000836increased protein levelWestern blot assaywis1-982 (F307S)PECO:0000090, PECO:0000005PMID:1936672836
affecting cdc13
FYPO:0001266normal protein phosphorylation during cellular response to salt stressWestern blot assaywis1ΔNullPMID:91351473
affecting pmk1
FYPO:0000280sterileMicroscopywis1ΔNullPECO:0000127, PECO:0000104PMID:967145839
FYPO:0000646swollen vegetative cellMicroscopywis1+ (wild type)OverexpressionPMID:750102435
FYPO:0000647vegetative cell lysisMicroscopywis1+ (wild type)OverexpressionPMID:750102436
FYPO:0001492viable elongated vegetative cellMicroscopywis1ΔNullPECO:0000137, PECO:0000005PMID:23697806170
expressivity FYPO_EXT:0000001
penetrance FYPO_EXT:0000001
Cell growth assaywis1ΔNullPECO:0000005PMID:1756736
FYPO:0000648viable small vegetative cellMicroscopywis1+ (wild type)OverexpressionPMID:175673628
Target Of
RelationshipGeneProductReference
substrate of wis4 MAP kinase kinase kinase Wis4 PMID:GO_REF:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
111466331143731

UTRs

Region Start End Reference
five_prime_UTR11466331145785PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 322 579 109
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 320 579 73
PS50011 Prosite Profiles IPR000719 Protein kinase domain 320 579 110
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 437 449 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 326 349 73
PTHR24360 HMMPANTHER 78 600 3
PTHR24360:SF3 HMMPANTHER 78 600 1
G3DSA:3.30.200.20 Gene3D 315 383 109
G3DSA:1.10.510.10 Gene3D 384 586 112
SSF56112 SuperFamily IPR011009 318 585 127
Low complexity (SEG) seg 31 73
Low complexity (SEG) seg 90 105
Low complexity (SEG) seg 128 140
Low complexity (SEG) seg 244 256

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0012556WIn SuppressingPMID:1756736Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0012556

Protein Properties

Ave. residue weight 107.05 Da
Charge 13.50
Isoelectric point 9.40
Molecular weight 64.76 kDa
Number of residues 605
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:182575171192
PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresentNorthern assay evidencePMID:1756736
during GO:0072690

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4753.27during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
7210.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
7.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
nrd1RNA-binding protein Nrd1 Phenotypic SuppressionPMID:9671458
tup11transcriptional corepressor Tup11 Phenotypic SuppressionPMID:11238405
Synthetic Growth Defect
ptc1protein phosphatase 2C Ptc1 Synthetic RescuePMID:7859738
leu13-isopropylmalate dehydrogenase Leu1 Phenotypic SuppressionPMID:15466417
pyp1tyrosine phosphatase Pyp1 Phenotypic EnhancementPMID:9321395
sds23PP2A-type phosphatase inhibitor Sds23/Moc1 Phenotypic EnhancementPMID:23640764
Dosage Lethality
lsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Phenotypic SuppressionPMID:22144909
cmk2MAPK-activated protein kinase Cmk2 Synthetic RescuePMID:11886858
ptc4protein phosphatase 2C Ptc4 Phenotypic EnhancementPMID:22139357
cdc25M phase inducer phosphatase Cdc25 Dosage RescuePMID:1756736
Phenotypic Suppression
Synthetic LethalityPMID:9843572
Phenotypic SuppressionPMID:8557102
cut1separase/separin Synthetic RescuePMID:16861909
srk1MAPK-activated protein kinase Srk1 Synthetic RescuePMID:12080074
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:1756736
sty1MAP kinase Sty1 Synthetic RescuePMID:9450957
Phenotypic SuppressionPMID:7501024
Phenotypic SuppressionPMID:8649397
Dosage RescuePMID:8557102
pmk1MAP kinase Pmk1 Phenotypic EnhancementPMID:9135147
wis4MAP kinase kinase kinase Wis4 Synthetic RescuePMID:9450957
Synthetic RescuePMID:9321395
Dosage RescuePMID:9693384
Phenotypic Suppression
tup12transcriptional corepressor Tup12 Synthetic Growth DefectPMID:11238405
Phenotypic Suppression
ppa1minor serine/threonine protein phosphatase Ppa1 Phenotypic SuppressionPMID:1756736
ptc3protein phosphatase 2c homolog 3 Synthetic RescuePMID:7859738
wee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:1756736
Dosage Rescue
cyr1adenylate cyclase Synthetic RescuePMID:15189983
gti1gluconate transporter inducer Gti1 Phenotypic SuppressionPMID:9372449
mcs4response regulator Mcs4 Dosage RescuePMID:9136929
Synthetic Rescue
atf1transcription factor, Atf-CREB family Atf1 Synthetic RescuePMID:8824587
Phenotypic SuppressionPMID:15870269
pka1cAMP-dependent protein kinase catalytic subunit Pka1 Synthetic Growth DefectPMID:23640764
gpd2glycerol-3-phosphate dehydrogenase Gpd2 Dosage RescuePMID:7498541
win1MAP kinase kinase kinase Win1 Dosage RescuePMID:1756736
Synthetic RescuePMID:9450957
Dosage Rescue
Synthetic RescuePMID:9321395
Dosage RescuePMID:9693384
Phenotypic Suppression
sts5RNB-like protein Synthetic RescuePMID:8886983
pck2protein kinase C (PKC)-like Pck2 Phenotypic SuppressionPMID:9199286
gpd1glycerol-3-phosphate dehydrogenase Gpd1 Dosage RescuePMID:7498541
cdc37Hsp90 co-chaperone Cdc37 Synthetic RescuePMID:12861001
isp6vacuolar serine protease Isp6 Phenotypic EnhancementPMID:16550352
ran1serine/threonine protein kinase Ran1 Synthetic RescuePMID:8832415
mkh1MEK kinase (MEKK) Mkh1 Phenotypic SuppressionPMID:9199286
Phenotypic Enhancement
byr1MAP kinase kinase Byr1 Synthetic Growth DefectPMID:8557102
ppa2serine/threonine protein phosphatase Ppa2 Phenotypic SuppressionPMID:1756736
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
mpr1histidine-containing response regulator phosphotransferase Mpr1 Two-hybridPMID:23695164
sty1MAP kinase Sty1 Affinity Capture-WesternPMID:12181336
Biochemical ActivityPMID:9718372
Biochemical ActivityPMID:7501024
Biochemical ActivityPMID:10398679
Two-hybridPMID:23695164
win1MAP kinase kinase kinase Win1 Biochemical ActivityPMID:9693384
Affinity Capture-LuminescencePMID:23389634
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
wis4MAP kinase kinase kinase Wis4 Biochemical ActivityPMID:9718372
Biochemical ActivityPMID:9321395
Affinity Capture-LuminescencePMID:23389634
External References
Database Identifier Description
NBRP SPBC409.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC409.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC409.07c BioGRID Interaction Datasets
Expression Viewer SPBC409.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC409.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC409.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC409.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC409.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC409.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC409.07c Cell Cycle Data
GEO SPBC409.07c GEO profiles
PInt SPBC409.07c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2541055MAP kinase kinase Wis1
EntrezGene2541055MAP kinase kinase Wis1
SPD / RIKEN33/33D12Orfeome Localization Data
UniProtKB/SwissProtP33886Protein kinase wis1
ModBaseP33886Database of comparative protein structure models
StringP33886Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595457MAP kinase kinase Wis1
RefSeq mRNANM_001021367972h- MAP kinase kinase Wis1 (wis1), mRNA
European Nucleotide ArchiveCAA44499ENA Protein Mapping
European Nucleotide ArchiveCAB52609ENA Protein Mapping
UniParcUPI0000138F12UniProt Archive

Literature for wis1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014