SPBC428.15


Gene Standard NameUnassigned Characterisation Statusconserved unknown
Systematic IDSPBC428.15 Feature Typeprotein coding
Synonyms Name Description
ProductObg-like ATPase (predicted) Product Size409aa, 44.46 kDa
Genomic Location Chromosome II, 472084-473687 (1604nt); CDS:472210-473439 (1230nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding557
Annotation ExtensionEvidenceWith/FromReference
ATPase activity238
Annotation ExtensionEvidenceWith/FromReference
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationSPBC428.15Δ3850

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologySPBC428.15Δ3102
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons472084..473687
mRNA472084..473687
5' UTR472084..472209PMID:21511999
CDS472210..473439
3' UTR473440..473687PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08438 Pfam IPR013646 Uncharacterised GTP-binding protein, C-terminal 226 339 1
PF01926 Pfam IPR006073 GTP binding domain 7 124 12
PS51710 Prosite Profiles IPR031167 OBG-type guanine nucleotide-binding (G) domain 5 274 7
PTHR23305 HMMPANTHER 1 367 3
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 221 280 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 6 153 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 7 317 294
PR00326 PRINTS IPR006073 GTP binding domain 7 27 9
PR00326 PRINTS IPR006073 GTP binding domain 28 46 9
PR00326 PRINTS IPR006073 GTP binding domain 81 96 9
PR00326 PRINTS IPR006073 GTP binding domain 98 116 9

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000997GTP1/OBG familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000997

Protein Properties

Ave. residue weight 108.71 Da
Charge 3.50
Codon Adaptation Index 0.39
Isoelectric point 7.24
Molecular weight 44.46 kDa
Number of residues 409
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for SPBC428.15

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
18062during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14717during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
15099during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14997during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9018.52during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
16111during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2140.94during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.3during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.52during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC428.15 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withcfr1Chs five related protein Cfr1 Two-hybridPMID:26771498
binds DNA-binding domain construct withrix7AAA family ATPase Rix7 (predicted) Two-hybridPMID:26771498
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPBC428.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC428.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC428.15 BioGRID Interaction Datasets
Expression Viewer SPBC428.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC428.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC428.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC428.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC428.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC428.15 Transcriptome Viewer (Bähler Lab)
GEO SPBC428.15 GEO profiles
PInt SPBC428.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC428.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC428.15 Fission yeast phenotypic data & analysis
Cyclebase SPBC428.15.1 Cell Cycle Data
SPD / RIKEN19/19F01Orfeome Localization Data
UniProtKB/SwissProtO94362Uncharacterized GTP-binding protein C428.15
ModBaseO94362Database of comparative protein structure models
STRINGO94362Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595193Obg-like ATPase (predicted)
RefSeq mRNANM_001021100972h- Obg-like ATPase (predicted) (SPBC428.15), mRNA
European Nucleotide ArchiveCAA22290.1ENA Protein Mapping
UniParcUPI000006B77DUniProt Archive

Literature for SPBC428.15

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016