SPBC428.15


Gene Standard NameUnassigned Characterisation Statusconserved_unknown
Systematic IDSPBC428.15 Feature Typeprotein coding
Synonyms Name Description
ProductObg-like ATPase (predicted) Product Size409aa, 44.46 kDa
Genomic Location Chromosome II, 472084-473687 (1604nt); CDS:472210-473439 (1230nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingISSUniProtKB:Q9NTK5GO_REF:0000024558
GO:0016887ATPase activityISSUniProtKB:Q9NTK5GO_REF:0000024232
GO:0005525GTP bindingIEAUniProtKB-KW:KW-0342GO_REF:0000037113
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000003442GO_REF:00000244205
GO:0005829cytosolIDAPMID:168233722315
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopySPBC428.15ΔNullPECO:0000005, PECO:0000137PMID:236978063759
MicroscopySPBC428.15ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001MicroscopySPBC428.15ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1472084473687

UTRs

Region Coordinates Reference
five_prime_UTR472084..472209PMID:21511999
three_prime_UTR473440..473687PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08438 Pfam IPR013646 Uncharacterised GTP-binding protein, C-terminal 226 339 1
PF01926 Pfam IPR006073 GTP binding domain 7 121 12
PTHR23305 HMMPANTHER 1 367 3
3.40.50.300 Gene3D 221 280 288
3.40.50.300 Gene3D 6 153 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 7 317 294
PR00326 PRINTS IPR006073 GTP binding domain 81 96 9
PR00326 PRINTS IPR006073 GTP binding domain 28 46 9
PR00326 PRINTS IPR006073 GTP binding domain 98 116 9
PR00326 PRINTS IPR006073 GTP binding domain 7 27 9

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000997GTP1/OBG familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000997

Protein Properties

Ave. residue weight 108.71 Da
Charge 3.50
Isoelectric point 7.24
Molecular weight 44.46 kDa
Number of residues 409
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
18062during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
14717during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
15099during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
14997during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
16111during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
9018.52during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2140.94during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.3during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.52during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPBC428.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC428.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC428.15 BioGRID Interaction Datasets
Expression Viewer SPBC428.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC428.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC428.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC428.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC428.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC428.15 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC428.15 Cell Cycle Data
GEO SPBC428.15 GEO profiles
PInt SPBC428.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC428.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC428.15 Fission yeast phenotypic data & analysis
SPD / RIKEN19/19F01Orfeome Localization Data
UniProtKB/SwissProtO94362Uncharacterized GTP-binding protein C428.15
ModBaseO94362Database of comparative protein structure models
STRINGO94362Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595193Obg-like ATPase (predicted)
RefSeq mRNANM_001021100972h- Obg-like ATPase (predicted) (SPBC428.15), mRNA
European Nucleotide ArchiveCAA22290.1ENA Protein Mapping
UniParcUPI000006B77DUniProt Archive

Literature for SPBC428.15

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014