ght2 (SPBC4B4.08)


Gene Standard Nameght2 Characterisation Statuspublished
Systematic IDSPBC4B4.08 Feature Typeprotein coding
Synonyms Name Description
Producthexose transmembrane transporter Ght2 Product Size531aa, 58.85 kDa
Genomic Location Chromosome II, 3427180-3429424 (2245nt); CDS:3427572-3429167 (1596nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
GO Cellular Component
Term NameCount
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
integral component of plasma membrane49
Annotation ExtensionEvidenceWith/FromReference
plasma membrane253
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
normal cell population growth during glucose starvationght2Δ8
normal growth on glucose carbon sourceght2Δ294
viable vegetative cell populationght2Δ3850

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyght2Δ3102

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growth on glucose carbon sourceght8Δ, ght1Δ, ght2Δ, ght5Δ
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3427180..3429424
mRNA3427180..3429424
5' UTR3427180..3427571AF017180
CDS3427572..3429167
3' UTR3429168..3429424AF017180
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00083 Pfam IPR005828 Major facilitator, sugar transporter-like 15 471 15
TMhelix TMHMM 308 330 950
TMhelix TMHMM 372 394 950
TMhelix TMHMM 62 84 950
TMhelix TMHMM 91 110 950
TMhelix TMHMM 271 293 950
TMhelix TMHMM 335 357 950
TMhelix TMHMM 9 31 950
TMhelix TMHMM 407 429 950
TMhelix TMHMM 149 171 950
TMhelix TMHMM 181 203 950
TMhelix TMHMM 114 136 950
TMhelix TMHMM 433 455 950
PS00216 Prosite Patterns IPR005829 Sugar transporter, conserved site 322 339 15
PS50850 Prosite Profiles IPR020846 Major facilitator superfamily domain 13 460 55
PTHR23500:SF131 HMMPANTHER 9 529 8
PTHR23500 HMMPANTHER 9 529 10
1.20.1250.20 Gene3D Major facilitator superfamily domain 53 214 72
1.20.1250.20 Gene3D Major facilitator superfamily domain 267 466 72
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 249 469 76
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 17 218 76
SignalP-noTM signalp 1 25 207
PR00171 PRINTS IPR003663 Sugar/inositol transporter 21 31 10
PR00171 PRINTS IPR003663 Sugar/inositol transporter 396 408 10
PR00171 PRINTS IPR003663 Sugar/inositol transporter 280 290 10
PR00171 PRINTS IPR003663 Sugar/inositol transporter 373 394 10
PR00171 PRINTS IPR003663 Sugar/inositol transporter 118 137 10
TIGR00879 TIGRFAM IPR003663 Sugar/inositol transporter 9 467 10

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.82 Da
Charge 8.50
Codon Adaptation Index 0.68
Isoelectric point 8.55
Molecular weight 58.85 kDa
Number of residues 531
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS507
S507, S515, S519, S520, S524, S525
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S507 PMID:21712547
IDA S525 PMID:25720772
IDA S507 PMID:25720772
experimental evidence S524 PMID:24763107
experimental evidence S515 PMID:24763107
experimental evidence S519 PMID:24763107
IDA S524 PMID:25720772
experimental evidence S507 PMID:24763107
IDA S520 PMID:25720772
IDA S515 PMID:25720772
O-phospho-L-threonineT517 1085
Annotation ExtensionEvidenceResidueReference
IDA T517 PMID:25720772
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
O-phosphorylated residueS507,S515, S515,S519, S515,S519,S524, S515,S519,S525, S515,S519,Y523, S515,S520, S515,Y523, S519,S524, S519,S525, S519,Y523, S520,S524, S520,S525, T517,S519, T517,S519,Y523, T517,S520, Y523,S525 2457
Annotation ExtensionEvidenceResidueReference
IDA S507,S515 PMID:25720772
IDA S520,S524 PMID:25720772
IDA T517,S519 PMID:25720772
IDA S515,S519,S525 PMID:25720772
IDA S515,S519,Y523 PMID:25720772
IDA S519,S525 PMID:25720772
IDA S515,Y523 PMID:25720772
IDA S515,S519,S524 PMID:25720772
IDA S515,S520 PMID:25720772
IDA S515,S519 PMID:25720772
IDA S520,S525 PMID:25720772
IDA T517,S519,Y523 PMID:25720772
IDA S519,S524 PMID:25720772
IDA S519,Y523 PMID:25720772
IDA T517,S520 PMID:25720772
IDA Y523,S525 PMID:25720772
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein leveldecreased during GO:0042149Western blot evidencePMID:25411338
present during GO:0072690Western blot evidencePMID:25411338
RNA leveldecreased during GO:0042149quantitative PCRPMID:25411338

Quantitative Gene Expression

View graphical display of gene expression data for ght2 (SPBC4B4.08)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
20186during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
19517during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
20847during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
20914during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
19792during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
10312.95during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2313.77during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC4B4.08 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withuge1UDP-glucose 4-epimerase Uge1 Synthetic Growth DefectPMID:23254763
External References
Database Identifier Description
NBRP SPBC4B4.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC4B4.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC4B4.08 BioGRID Interaction Datasets
Expression Viewer SPBC4B4.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC4B4.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC4B4.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC4B4.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC4B4.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC4B4.08 Transcriptome Viewer (Bähler Lab)
GEO SPBC4B4.08 GEO profiles
PInt SPBC4B4.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC4B4.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC4B4.08 Fission yeast phenotypic data & analysis
Cyclebase SPBC4B4.08.1 Cell Cycle Data
SPD / RIKEN40/40F03Orfeome Localization Data
UniProtKB/SwissProtO74969High-affinity glucose transporter ght2
ModBaseO74969Database of comparative protein structure models
STRINGO74969Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596425hexose transporter Ght2
RefSeq mRNANM_001022344972h- hexose transporter Ght2 (ght2), mRNA
European Nucleotide ArchiveAF017180ENA EMBL mapping
European Nucleotide ArchiveCAA19288.1ENA Protein Mapping
UniParcUPI000012B41FUniProt Archive

Literature for ght2

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016