pre3 (SPBC4C3.10c)


Gene Standard Namepre3 Characterisation Statusbiological_role_inferred
Systematic IDSPBC4C3.10c Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit beta 1 Pre3 (predicted) Product Size226aa, 24.56 kDa
Genomic Location Chromosome II, 3117052-3118883 (1832nt); CDS:3117669-3118627 (959nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004175endopeptidase activity66
contributes_toISOSGD:S000003538GO_REF:0000024
GO:0004298threonine-type endopeptidase activityIEAUniProtKB-KW:KW-0888GO_REF:000003715
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0043161proteasome-mediated ubiquitin-dependent protein catabolic processNASGO_REF:0000001110
GO:0007346regulation of mitotic cell cycleNASGO_REF:0000051281
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0005634nucleusIDAPMID:168233722740
GO:0019774proteasome core complex, beta-subunit complexISOSGD:S000003538GO_REF:00000247
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopypre3ΔNullPECO:0000005, PECO:0000137PMID:236978061331
Microscopypre3ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphologyMicroscopypre3ΔNullPECO:0000005, PECO:0000137PMID:23697806237
FYPO:0002151inviable spore459
penetrance FYPO_EXT:0000001Microscopypre3ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
131170523117724
231177683117850
331179033118035
431182193118883

UTRs

Region Coordinates Reference
five_prime_UTR3117052..3117668PMID:21511999
three_prime_UTR3118628..3118883PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 22 202 14
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 28 75 8
PS51476 Prosite Profiles IPR023333 Proteasome B-type subunit 24 203 7
PTHR11599:SF4 HMMPANTHER 1 221 1
PTHR11599 HMMPANTHER 1 221 14
3.60.20.10 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 25 211 19
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 19 210 22
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 164 175 2
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 189 200 2
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 153 164 2
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 32 47 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.69 Da
Charge 0.00
Isoelectric point 6.51
Molecular weight 24.56 kDa
Number of residues 226
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
104174during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
111043during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
92826during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
109255during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
108695during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
47839.27during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
20985.54during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
6.3during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
ubp6ubiquitin C-terminal hydrolase Ubp6 Affinity Capture-MSPMID:20838651
uch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
rpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPBC4C3.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC4C3.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC4C3.10c BioGRID Interaction Datasets
Expression Viewer SPBC4C3.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC4C3.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC4C3.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC4C3.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC4C3.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC4C3.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC4C3.10c Cell Cycle Data
GEO SPBC4C3.10c GEO profiles
PInt SPBC4C3.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC4C3.10c Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
EntrezGene254087820S proteasome component beta 1 Pre3 (predicted)
WikiGene254087820S proteasome component beta 1 Pre3 (predicted)
SPD / RIKEN13/13E12Orfeome Localization Data
UniProtKB/SwissProtO43063Probable proteasome subunit beta type-1
ModBaseO43063Database of comparative protein structure models
STRINGO43063Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59629520S proteasome component beta 1 Pre3 (predicted)
RefSeq mRNANM_001022216972h- 20S proteasome component beta 1 Pre3 (predicted) (pre3), mRNA
MEROPST01.010
European Nucleotide ArchiveCAA16832ENA Protein Mapping
European Nucleotide ArchiveCAA16832.1ENA Protein Mapping
ReactomeREACT_188524
ReactomeREACT_188566
ReactomeREACT_188568
ReactomeREACT_188587
ReactomeREACT_208775
ReactomeREACT_215140
ReactomeREACT_215320
ReactomeREACT_218991
ReactomeREACT_81558
UniParcUPI00001325C5UniProt Archive

Literature for pre3

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014