pre3 (SPBC4C3.10c)


Gene Standard Namepre3 Characterisation Statusbiological role inferred
Systematic IDSPBC4C3.10c Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit beta 1 Pre3 (predicted) Product Size226aa, 24.56 kDa
Genomic Location Chromosome II, 3117052-3118883 (1832nt); CDS:3117669-3118627 (959nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
endopeptidase activity66
Annotation ExtensionEvidenceWith/FromReference
threonine-type endopeptidase activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
proteasome core complex, beta-subunit complex7
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationpre3Δ1455

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination with normal, unseptated germ tube morphologypre3Δ237
inviable sporepre3Δ476
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3117052..3117724, 3117768..3117850, 3117903..3118035, 3118219..3118883
Intron3117725..3117767, 3117851..3117902, 3118036..3118218
mRNA3117052..3118883
5' UTR3117052..3117668PMID:21511999
CDS3117669..3117724, 3117768..3117850, 3117903..3118035, 3118219..3118627
3' UTR3118628..3118883PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 22 202 14
PS51476 Prosite Profiles IPR023333 Proteasome B-type subunit 24 203 7
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 28 75 8
PTHR11599 HMMPANTHER 20 216 14
PTHR11599:SF4 HMMPANTHER 20 216 1
3.60.20.10 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 25 211 19
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 19 210 22
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 189 200 2
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 164 175 2
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 153 164 2
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 32 47 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.69 Da
Charge 0.00
Codon Adaptation Index 0.51
Isoelectric point 6.51
Molecular weight 24.56 kDa
Number of residues 226
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS70 2289
Annotation ExtensionEvidenceResidueReference
IDA S70 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pre3 (SPBC4C3.10c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
104174during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
111043during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
92826during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
109255during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
47839.27during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
108695during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
20985.54during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.3during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in bacteria1005
conserved in metazoa3427
conserved in vertebrates3402
conserved in archaea242
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC4C3.10c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypre620S proteasome complex subunit alpha 4 Pre6 Affinity Capture-MSPMID:20133687
affinity captured byuch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
affinity captured byubp6ubiquitin C-terminal hydrolase Ubp6 Affinity Capture-MSPMID:20838651
affinity captured byrpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPBC4C3.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC4C3.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC4C3.10c BioGRID Interaction Datasets
Expression Viewer SPBC4C3.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC4C3.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC4C3.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC4C3.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC4C3.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC4C3.10c Transcriptome Viewer (Bähler Lab)
GEO SPBC4C3.10c GEO profiles
PInt SPBC4C3.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC4C3.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC4C3.10c Fission yeast phenotypic data & analysis
Cyclebase SPBC4C3.10c.1 Cell Cycle Data
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
SPD / RIKEN13/13E12Orfeome Localization Data
UniProtKB/SwissProtO43063Probable proteasome subunit beta type-1
ModBaseO43063Database of comparative protein structure models
STRINGO43063Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59629520S proteasome component beta 1 Pre3 (predicted)
RefSeq mRNANM_001022216972h- 20S proteasome component beta 1 Pre3 (predicted) (pre3), mRNA
European Nucleotide ArchiveCAA16832.1ENA Protein Mapping
UniParcUPI00001325C5UniProt Archive

Literature for pre3

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016