pre3 (SPBC4C3.10c)


Gene Standard Namepre3 Characterisation Statusbiological_role_inferred
Systematic IDSPBC4C3.10c Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit beta 1 Pre3 (predicted) Product Size226aa, 24.56 kDa
Genomic Location Chromosome II, 3117052-3118883 (1832nt); CDS:3117669-3118627 (959nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
endopeptidase activity65
Annotation ExtensionEvidenceWith/FromReference
threonine-type endopeptidase activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
positive regulation of mitotic metaphase/anaphase transition55
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
proteasome core complex, beta-subunit complex7
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationpre3ΔNull1428

Cell Phenotype

Term NameAlleleExpressionCount
inviable after spore germination with normal, unseptated germ tube morphologypre3ΔNull237
inviable sporepre3ΔNull468
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3117052..3117724, 3117768..3117850, 3117903..3118035, 3118219..3118883
mRNA3117052..3118883
5' UTR3117052..3117668PMID:21511999
CDS3117669..3117724, 3117768..3117850, 3117903..3118035, 3118219..3118627
3' UTR3118628..3118883PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 22 202 14
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 28 75 8
PS51476 Prosite Profiles IPR023333 Proteasome B-type subunit 24 203 7
PTHR11599 HMMPANTHER 1 221 14
PTHR11599:SF4 HMMPANTHER 1 221 1
3.60.20.10 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 25 211 19
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 19 210 22
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 32 47 2
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 189 200 2
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 153 164 2
PR00141 PRINTS IPR000243 Peptidase T1A, proteasome beta-subunit 164 175 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.69 Da
Charge 0.00
Isoelectric point 6.51
Molecular weight 24.56 kDa
Number of residues 226
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
104174during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
111043during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
92826during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
109255during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
47839.27during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
108695during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
20985.54during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3430
conserved in vertebrates3405
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
affinity captured bypre620S proteasome complex subunit alpha 4 Pre6 Affinity Capture-MSPMID:20133687
affinity captured byrpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
affinity captured byubp6ubiquitin C-terminal hydrolase Ubp6 Affinity Capture-MSPMID:20838651
affinity captured byuch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPBC4C3.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC4C3.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC4C3.10c BioGRID Interaction Datasets
Expression Viewer SPBC4C3.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC4C3.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC4C3.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC4C3.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC4C3.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC4C3.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC4C3.10c Cell Cycle Data
GEO SPBC4C3.10c GEO profiles
PInt SPBC4C3.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC4C3.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC4C3.10c Fission yeast phenotypic data & analysis
SPD / RIKEN13/13E12Orfeome Localization Data
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
UniProtKB/SwissProtO43063Probable proteasome subunit beta type-1
ModBaseO43063Database of comparative protein structure models
STRINGO43063Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59629520S proteasome component beta 1 Pre3 (predicted)
RefSeq mRNANM_001022216972h- 20S proteasome component beta 1 Pre3 (predicted) (pre3), mRNA
European Nucleotide ArchiveCAA16832.1ENA Protein Mapping
UniParcUPI00001325C5UniProt Archive

Literature for pre3

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015