pek1 (SPBC543.07)


Gene Standard Namepek1 Characterisation Statuspublished
Systematic IDSPBC543.07 Feature Typeprotein coding
Synonymsmkk1, skh1 Name DescriptionPombe mEK 1
ProductMAP kinase kinase Pek1 Product Size363aa, 40.71 kDa
Genomic Location Chromosome II, 4312422-4314140 (1719nt); CDS:4312763-4313854 (1092nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
MAP kinase kinase activity3
Annotation ExtensionEvidenceWith/FromReference
protein tyrosine kinase activity9
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
activation of MAPK activity3
Annotation ExtensionEvidenceWith/FromReference
cellular sodium ion homeostasis4
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall organization or biogenesis116
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade26
Annotation ExtensionEvidenceWith/FromReference
mitotic cytokinesis110
Annotation ExtensionEvidenceWith/FromReference
peptidyl-tyrosine phosphorylation12
Annotation ExtensionEvidenceWith/FromReference
positive regulation of calcium-mediated signaling involved in cellular response to salt stress4
Annotation ExtensionEvidenceWith/FromReference
regulation of cell shape73
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell septum25
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased bipolar indexpek1ΔNull27
increased monopolar indexpek1ΔNull35
increased viability upon nitrogen starvationpek1ΔNull54
normal growth on cell wall-degrading enzymespek1DD (S234D, T238D)Not specified8
resistance to hydrogen peroxidepek1ΔNull73
resistance to tacrolimus during salt stresspek1ΔNull5
sensitive to amphotericin Bpek1ΔNull69
sensitive to caffeine during vegetative growthpek1ΔNull106
sensitive to camptothecinpek1ΔNull225
sensitive to cell wall-degrading enzymespek1ΔNull44
pek1A (T238A)Not specified
sensitive to hydroxyureapek1ΔNull534
sensitive to micafunginpek1ΔNull116
sensitive to sodium butyratepek1ΔNull93
sensitive to trichostatin Apek1ΔNull43
sensitive to valproic acidpek1ΔNull155
viable vegetative cell populationpek1ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
abnormal cytokinesispek1ΔNull221
abolished protein threonine phosphorylation at Thr-Pro site2
affecting rnc1pek1A (T238A)Not specified
abolished protein tyrosine phosphorylation4
affecting pmk1pek1ΔNull
abolished RNA binding10
affecting pmp1 and rnc1pek1A (T238A)Not specified
decreased protein binding66
affecting pmk1 and pek1pek1DD (S234D, T238D)Not specified
decreased RNA catabolic process during vegetative growth10
affecting pmp1pek1DD (S234D, T238D)Not specified
decreased transcription from CDRE promoter in response to micafunginpek1ΔNull6
decreased transcription from CDRE promoter in response to salt stresspek1ΔNull6
elongated vegetative cellpek1ΔNull174
increased cellular calcium levelpek1DD (S234D, T238D)Overexpression8
increased protein binding12
affecting pmk1 and pek1pek1A (T238A)Not specified
increased protein threonine phosphorylation at Thr-Pro site1
affecting rnc1pek1DD (S234D, T238D)Not specified
increased protein tyrosine phosphorylation11
affecting pmk1pek1+ (wild type)Overexpression
affecting pmk1pek1DD (S234D, T238D)Overexpression
increased RNA binding1
affecting pmp1 and rnc1pek1DD (S234D, T238D)Not specified
increased RNA catabolic process during vegetative growth4
affecting pmp1pek1A (T238A)Not specified
increased transcription from CDRE promoter in response to calcium ionpek1DD (S234D, T238D)Overexpression6
vegetative cell lysispek1ΔNull83
viable elongated multiseptate vegetative cellpek1ΔNull11
viable stubby vegetative cellpek1ΔNull72
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in pek1 MAP kinase kinase Pek1
GO substrate of mkh1 MEK kinase (MEKK) Mkh1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4312422..4314140
mRNA4312422..4314140
5' UTR4312422..4312762PMID:21511999
CDS4312763..4313854
3' UTR4313855..4314140PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 83 343 109
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 79 343 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 79 343 110
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 202 214 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 85 108 73
PTHR24360 HMMPANTHER 62 361 3
PTHR24360:SF45 HMMPANTHER 62 361 1
3.30.200.20 Gene3D 82 146 111
1.10.510.10 Gene3D 147 344 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 77 348 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.14 Da
Charge 7.00
Isoelectric point 8.20
Molecular weight 40.71 kDa
Number of residues 363
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2962during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2769during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2983during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3727during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1360.69during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3209during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.76during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
Mkh1-Pek1-Spm1 MAP kinase PMID:105916343
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withhsp16heat shock protein Hsp16 Two-hybridPMID:23695164
binds DNA-binding domain construct withmkh1MEK kinase (MEKK) Mkh1 Two-hybridPMID:10591634
binds DNA-binding domain construct withmkh1MEK kinase (MEKK) Mkh1 Two-hybridPMID:23695164
modifiespmk1MAP kinase Pmk1 Biochemical ActivityPMID:10591634
affinity captured bypmk1MAP kinase Pmk1 Affinity Capture-WesternPMID:16291757
affinity capturespmk1MAP kinase Pmk1 Affinity Capture-WesternPMID:10365961
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withabo2ATPase with bromodomain protein (predicted) Positive GeneticPMID:22681890
negative genetic interaction withada1adenosine deaminase Ada1 Negative GeneticPMID:22681890
positive genetic interaction withadh4alcohol dehydrogenase Adh4 Positive GeneticPMID:22681890
positive genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
negative genetic interaction withapt1adenine phosphoribosyltransferase (APRT) (predicted) Negative GeneticPMID:22681890
positive genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Positive GeneticPMID:22681890
positive genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Positive GeneticPMID:18818364
positive genetic interaction withbun107WD repeat protein, human WDR48 family Bun107 Positive GeneticPMID:22681890
negative genetic interaction withcaf1CCR4-Not complex CAF1 family ribonuclease subunit Caf1 Negative GeneticPMID:18818364
rescuescch1calcium ion channel Cch1 Phenotypic SuppressionPMID:21811607
rescued bycch1calcium ion channel Cch1 Phenotypic SuppressionPMID:16928959
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:18818364
positive genetic interaction withcdm1DNA polymerase delta subunit Cdm1 Positive GeneticPMID:22681890
positive genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:18818364
positive genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
positive genetic interaction withcsn3COP9/signalosome complex subunit Csn3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcwf14G10 protein Positive GeneticPMID:22681890
positive genetic interaction withdad1DASH complex subunit Dad1 Positive GeneticPMID:22681890
positive genetic interaction withdbr1RNA lariat debranching enzyme Dbr1 Positive GeneticPMID:22681890
positive genetic interaction withdcr1dicer Positive GeneticPMID:18818364
negative genetic interaction withelp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withemc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
positive genetic interaction witherd1Erd1 homolog (predicted) Positive GeneticPMID:22681890
negative genetic interaction witherg28Erg28 protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction witherg5C-22 sterol desaturase Erg5 Negative GeneticPMID:22681890
negative genetic interaction withftp105Ubp5 interacting protein Ftp105 Negative GeneticPMID:22681890
positive genetic interaction withgmh4alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withgmh5alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:18818364
negative genetic interaction withgpa2heterotrimeric G protein alpha-2 subunit Gpa2 Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withidp1isocitrate dehydrogenase Idp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withint6eIF3e subunit Int6 Negative GeneticPMID:22681890
synthetically rescueskin1microtubule affinity-regulating kinase Kin1 Synthetic RescuePMID:23294323
positive genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
positive genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Positive GeneticPMID:18818364
overexpression rescuesmkh1MEK kinase (MEKK) Mkh1 Dosage RescuePMID:10591634
synthetic growth defect withmkh1MEK kinase (MEKK) Mkh1 Synthetic Growth DefectPMID:10365961
positive genetic interaction withmod21gamma tubulin complex subunit Mod21 Positive GeneticPMID:22681890
negative genetic interaction withmoe1translation initiation factor eIF3d Moe1 Negative GeneticPMID:22681890
negative genetic interaction withmpc1mitochondrial pyruvate transmembrane transporter subunit Mpc1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmpc2mitochondrial pyruvate transmembrane transporter Mpc2 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withmph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
negative genetic interaction withmso1endocytic docking protein Mso1 Negative GeneticPMID:22681890
negative genetic interaction withmsy1MS calcium ion channel protein Msy1 Negative GeneticPMID:22681890
negative genetic interaction withmug122PX/PXA domain protein Negative GeneticPMID:22681890
positive genetic interaction withmug20Schizosaccharomyces specific protein Mug20 Positive GeneticPMID:22681890
negative genetic interaction withmug80cyclin Clg1 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withmug89CDC50 domain protein, implicated in signal transduction (predicted) Positive GeneticPMID:22681890
negative genetic interaction withnmt14-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase Nmt1 Negative GeneticPMID:22681890
negative genetic interaction withnot2CCR4-Not complex subunit Not2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withpar1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
synthetically rescuespck2protein kinase C (PKC)-like Pck2 Synthetic RescuePMID:17005909
negative genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
rescued by overexpression ofpmk1MAP kinase Pmk1 Dosage RescuePMID:10591634
rescued bypmk1MAP kinase Pmk1 Phenotypic SuppressionPMID:16928959
overexpression rescuespmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:12931193
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:18818364
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
overexpression rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:12931193
synthetically rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:17005909
synthetically rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:10365961
synthetic growth defect withppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic Growth DefectPMID:10365961
overexpression rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:10365961
positive genetic interaction withppm1leucine carboxyl methyltransferase, involved in regulation of autophagy Ppm1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withppr5mitochondrial PPR repeat protein Ppr5 Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withpsy2protein phosphatase PP4 complex subunit Psy2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpth4mitochondrial translation release factor Negative GeneticPMID:22681890
positive genetic interaction withraf2Rik1-associated factor Raf2 Positive GeneticPMID:22681890
synthetically rescuesrho2Rho family GTPase Rho2 Synthetic RescuePMID:17005909
overexpression rescuesrnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Dosage RescuePMID:12931193
negative genetic interaction withrok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpl430260S ribosomal protein L37a (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpp20160S acidic ribosomal protein A2 Negative GeneticPMID:22681890
positive genetic interaction withrud3Golgi matrix protein Rud3 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withscs7sphingosine hydroxylase Scs7 Negative GeneticPMID:18818364
negative genetic interaction withski2Ski complex RNA helicase Ski2 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withsnz1pyridoxine biosynthesis protein Positive GeneticPMID:22681890
positive genetic interaction withspa1ornithine decarboxylase antizyme with +1 programmed ribosomal frameshift Spa1 Positive GeneticPMID:22681890
positive genetic interaction withspa2cell polarity protein Spa2 Positive GeneticPMID:22681890
positive genetic interaction withSPAC144.05ATP-dependent DNA helicase/ ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPAC17H9.08mitochondrial coenzyme A transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC19A8.11cconserved eukaryotic protein Negative GeneticPMID:22681890
positive genetic interaction withSPAC23G3.05cregulator of G-protein signaling (RGS) domain (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPAC4A8.14ribose-phosphate pyrophosphokinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC694.02DEAD/DEAH box helicase Negative GeneticPMID:22681890
negative genetic interaction withSPAC750.08cNAD-dependent malic enzyme (predicted), partial Negative GeneticPMID:22681890
positive genetic interaction withSPAC9G1.07Schizosaccharomyces specific protein Positive GeneticPMID:22681890
negative genetic interaction withSPBC1348.03S. pombe specific 5Tm protein family Negative GeneticPMID:22681890
negative genetic interaction withSPBC365.16conserved protein Negative GeneticPMID:22681890
negative genetic interaction withSPBC725.01aspartate aminotransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC839.02arrestin Aly1 related, implicated in endocytosis Negative GeneticPMID:22681890
negative genetic interaction withSPBPB7E8.01Schizosaccharomyces specific protein, predicted GPI anchor Negative GeneticPMID:22681890
negative genetic interaction withSPCC285.05purine nucleoside transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC622.03cdubious Negative GeneticPMID:22681890
positive genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
positive genetic interaction withswd2Set1C complex subunit Swd2.1 Positive GeneticPMID:18818364
positive genetic interaction withswi6HP1 family chromodomain protein Swi6 Positive GeneticPMID:22681890
negative genetic interaction withtcg1single-stranded telomeric binding protein Tgc1 Negative GeneticPMID:22681890
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withtma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtsc1hamartin Negative GeneticPMID:22681890
negative genetic interaction withubp3ubiquitin C-terminal hydrolase Ubp3 Negative GeneticPMID:18818364
negative genetic interaction withubp4ubiquitin C-terminal hydrolase Ubp4 Negative GeneticPMID:22681890
negative genetic interaction withulp1SUMO deconjugating enzyme Ulp1 Negative GeneticPMID:22681890
rescued byyam8stretch-activated calcium ion channel Yam8 Phenotypic SuppressionPMID:16928959
positive genetic interaction withyip5Rab GTPase binding Yip5 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
negative genetic interaction withzds1zds family protein phosphatase type A regulator Zds1 (predicted) Negative GeneticPMID:18818364
External References
Database Identifier Description
NBRP SPBC543.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC543.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC543.07 BioGRID Interaction Datasets
Expression Viewer SPBC543.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC543.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC543.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC543.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC543.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC543.07 Transcriptome Viewer (Bähler Lab)
GEO SPBC543.07 GEO profiles
PInt SPBC543.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC543.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC543.07 Fission yeast phenotypic data & analysis
Cyclebase SPBC543.07.1 Cell Cycle Data
SPD / RIKEN32/32E04Orfeome Localization Data
UniProtKB/SwissProtQ9Y884MAP kinase kinase skh1/pek1
ModBaseQ9Y884Database of comparative protein structure models
STRINGQ9Y884Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596795MAP kinase kinase Pek1
RefSeq mRNANM_001023815972h- MAP kinase kinase Pek1 (pek1), mRNA
European Nucleotide ArchiveAAD41399.1ENA Protein Mapping
European Nucleotide ArchiveBAA82312.1ENA Protein Mapping
European Nucleotide ArchiveCAC05249.1ENA Protein Mapping
UniParcUPI00001359D9UniProt Archive

Literature for pek1

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015