pek1 (SPBC543.07)


Gene Standard Namepek1 Characterisation Statuspublished
Systematic IDSPBC543.07 Feature Typeprotein coding
Synonymsmkk1, skh1 Name DescriptionPombe mEK 1
ProductMAP kinase kinase Pek1 Product Size363aa, 40.71 kDa
Genomic Location Chromosome II, 4312422-4314140 (1719nt); CDS:4312763-4313854 (1092nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding557
Annotation ExtensionEvidenceWith/FromReference
MAP kinase kinase activity3
Annotation ExtensionEvidenceWith/FromReference
protein binding887
Annotation ExtensionEvidenceWith/FromReference
protein tyrosine kinase activity9
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular sodium ion homeostasis4
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall organization or biogenesis96
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade27
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade involved in cell wall organization or biogenesis2
Annotation ExtensionEvidenceWith/FromReference
mitotic cytokinesis104
Annotation ExtensionEvidenceWith/FromReference
positive regulation of calcium-mediated signaling involved in cellular response to salt stress4
Annotation ExtensionEvidenceWith/FromReference
positive regulation of MAP kinase activity1
Annotation ExtensionEvidenceWith/FromReference
regulation of cell shape70
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
barrier septum25
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased bipolar indexpek1Δ27
decreased mating efficiencypek1Δ275
increased monopolar indexpek1Δ38
increased viability upon nitrogen starvationpek1Δ54
normal growth on cell wall-degrading enzymespek1DD (S234D, T238D)11
resistance to hydrogen peroxidepek1Δ75
resistance to tacrolimus during salt stresspek1Δ8
sensitive to amphotericin Bpek1Δ69
sensitive to beta-glucanasepek1Δ9
sensitive to caffeine during vegetative growthpek1Δ110
sensitive to camptothecinpek1Δ257
sensitive to cell wall-degrading enzymespek1Δ47
pek1A (T238A)
sensitive to hydroxyureapek1Δ594
sensitive to micafunginpek1Δ119
sensitive to salt stresspek1Δ99
sensitive to sodium butyratepek1Δ93
sensitive to trichostatin Apek1Δ44
sensitive to valproic acidpek1Δ155
viable vegetative cell populationpek1Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal cytokinesispek1Δ340
abolished protein phosphorylation during vegetative growth55
affecting pmk1pek1Δ
abolished protein threonine phosphorylation at Thr-Pro site2
affecting rnc1pek1A (T238A)
abolished protein tyrosine phosphorylation4
affecting pmk1pek1Δ
abolished RNA binding10
affecting pmp1 and rnc1pek1A (T238A)
decreased protein binding99
affecting pmk1 and pek1pek1DD (S234D, T238D)
decreased RNA catabolic process during vegetative growth10
affecting pmp1pek1DD (S234D, T238D)
decreased transcription from CDRE promoter in response to micafunginpek1Δ6
decreased transcription from CDRE promoter in response to salt stresspek1Δ6
elongated cell during cellular response to caffeinepek1Δ4
elongated vegetative cellpek1Δ802
increased cellular calcium levelpek1DD (S234D, T238D)8
increased protein binding18
affecting pmk1 and pek1pek1A (T238A)
increased protein phosphorylation during vegetative growth65
affecting pmk1pek1DD (S234D, T238D)
affecting atf1pek1DD (S234D, T238D)
increased protein threonine phosphorylation at Thr-Pro site1
affecting rnc1pek1DD (S234D, T238D)
increased protein tyrosine phosphorylation11
affecting pmk1pek1+
affecting pmk1pek1DD (S234D, T238D)
increased RNA binding1
affecting pmp1 and rnc1pek1DD (S234D, T238D)
increased RNA catabolic process during vegetative growth4
affecting pmp1pek1A (T238A)
increased transcription from CDRE promoter in response to calcium ionpek1DD (S234D, T238D)6
vegetative cell lysispek1Δ98
viable elongated multiseptate vegetative cellpek1Δ14
viable stubby vegetative cellpek1Δ75

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
normal growth on cell wall-degrading enzymesmkh1Δ, pek1DD (S234D, T238D)
sensitive to beta-glucanasepek1Δ, pmk1+
pek1DD (S234D, T238D), pmk1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in pek1 MAP kinase kinase Pek1
GO substrate of mkh1 MEK kinase (MEKK) Mkh1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4312422..4314140
mRNA4312422..4314140
5' UTR4312422..4312762PMID:21511999
CDS4312763..4313854
3' UTR4313855..4314140PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 83 343 108
SM00220 SMART IPR000719 Protein kinase domain 79 343 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 79 343 110
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 85 108 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 202 214 97
PTHR24361:SF351 HMMPANTHER 16 361 1
PTHR24361 HMMPANTHER 16 361 14
3.30.200.20 Gene3D Tyrosine-protein kinase, receptor class V, conserved site 82 146 111
1.10.510.10 Gene3D Aminoglycoside phosphotransferase 147 344 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 77 348 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.14 Da
Charge 7.00
Codon Adaptation Index 0.38
Isoelectric point 8.20
Molecular weight 40.71 kDa
Number of residues 363
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pek1 (SPBC543.07)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2962during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2769during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2983during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3727during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1360.69during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3209during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.76during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
Mkh1-Pek1-Spm1 MAP kinase PMID:105916343
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC543.07 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withcnd2condensin complex non-SMC subunit Cnd2 Two-hybridPMID:26771498
binds DNA-binding domain construct withmkh1MEK kinase (MEKK) Mkh1 Two-hybridPMID:23695164
binds DNA-binding domain construct withhsp16heat shock protein Hsp16 Two-hybridPMID:23695164
affinity captured bypmk1MAP kinase Pmk1 Affinity Capture-WesternPMID:26432170
affinity capturespmk1MAP kinase Pmk1 Affinity Capture-WesternPMID:10365961
modifiespmk1MAP kinase Pmk1 Biochemical ActivityPMID:10591634
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBC543.07 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withapt1adenine phosphoribosyltransferase (APRT) (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withnot2CCR4-Not complex subunit Not2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withclg1cyclin Clg1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtsc1hamartin Negative GeneticPMID:22681890
negative genetic interaction withzds1zds family protein phosphatase type A regulator Zds1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction witherg5C-22 sterol desaturase Erg5 Negative GeneticPMID:22681890
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
negative genetic interaction withelp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC365.16conserved protein Negative GeneticPMID:22681890
negative genetic interaction withrpl430260S ribosomal protein L37a (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC750.08cNAD-dependent malic enzyme (predicted), partial Negative GeneticPMID:22681890
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
negative genetic interaction witherg28Erg28 protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpsy2protein phosphatase PP4 complex subunit Psy2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPCC285.05purine nucleoside transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtcg1single-stranded telomeric binding protein Tgc1 Negative GeneticPMID:22681890
negative genetic interaction withftp105Ubp5 interacting protein Ftp105 Negative GeneticPMID:22681890
negative genetic interaction withnmt14-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase Nmt1 Negative GeneticPMID:22681890
negative genetic interaction withmaa1mitochondrial aspartate aminotransferase Maa1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC622.03cSchizosaccharomyces pombe specific protein Negative GeneticPMID:22681890
negative genetic interaction withrok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC19A8.11cconserved eukaryotic protein Negative GeneticPMID:22681890
negative genetic interaction withscs7sphingosine hydroxylase Scs7 Negative GeneticPMID:18818364
negative genetic interaction withmoe1translation initiation factor eIF3d Moe1 Negative GeneticPMID:22681890
negative genetic interaction withint6eIF3e subunit Int6 Negative GeneticPMID:22681890
negative genetic interaction withrpp20160S acidic ribosomal protein A2 Negative GeneticPMID:22681890
negative genetic interaction withgpa2heterotrimeric G protein alpha-2 subunit Gpa2 Negative GeneticPMID:22681890
negative genetic interaction withemc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
negative genetic interaction withada1adenosine deaminase Ada1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC839.02arrestin Aly1 related, implicated in endocytosis Negative GeneticPMID:22681890
negative genetic interaction withSPAC694.02DEAD/DEAH box helicase Negative GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Negative GeneticPMID:22681890
negative genetic interaction withppr5mitochondrial PPR repeat protein Ppr5 Negative GeneticPMID:22681890
negative genetic interaction withubp4ubiquitin C-terminal hydrolase Ubp4 Negative GeneticPMID:22681890
negative genetic interaction withmpc1mitochondrial pyruvate transmembrane transporter subunit Mpc1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcaf1CCR4-Not complex CAF1 family ribonuclease subunit Caf1 Negative GeneticPMID:18818364
negative genetic interaction withubp3ubiquitin C-terminal hydrolase Ubp3 Negative GeneticPMID:18818364
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withidp1isocitrate dehydrogenase Idp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
negative genetic interaction withski2Ski complex RNA helicase Ski2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmpc2mitochondrial pyruvate transmembrane transporter Mpc2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC1348.03S. pombe specific 5Tm protein family Negative GeneticPMID:22681890
negative genetic interaction withtma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmug122PX/PXA domain protein Negative GeneticPMID:22681890
negative genetic interaction withSPBPB7E8.01Schizosaccharomyces specific protein, predicted GPI anchor Negative GeneticPMID:22681890
negative genetic interaction withSPAC17H9.08mitochondrial coenzyme A transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withulp1SUMO deconjugating enzyme Ulp1 Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withmso1exocytic docking protein Mso1 Negative GeneticPMID:22681890
negative genetic interaction withmsy1MS calcium ion channel protein Msy1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC4A8.14ribose-phosphate pyrophosphokinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpth4mitochondrial translation release factor Negative GeneticPMID:22681890
positive genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Positive GeneticPMID:22681890
positive genetic interaction withsnz1pyridoxine biosynthesis protein Positive GeneticPMID:22681890
positive genetic interaction withdcr1dicer Positive GeneticPMID:18818364
positive genetic interaction withmph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
positive genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
positive genetic interaction withdad1DASH complex subunit Dad1 Positive GeneticPMID:22681890
positive genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Positive GeneticPMID:18818364
positive genetic interaction withswi6HP1 family chromodomain protein Swi6 Positive GeneticPMID:22681890
positive genetic interaction withcsn3COP9/signalosome complex subunit Csn3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPAC9G1.07Schizosaccharomyces specific protein Positive GeneticPMID:22681890
positive genetic interaction withppm1leucine carboxyl methyltransferase, involved in regulation of autophagy Ppm1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
positive genetic interaction withcdm1DNA polymerase delta subunit Cdm1 Positive GeneticPMID:22681890
positive genetic interaction withswd2Set1C complex subunit Swd2.1 Positive GeneticPMID:18818364
positive genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Positive GeneticPMID:18818364
positive genetic interaction withcwf14G10 protein Positive GeneticPMID:22681890
positive genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:22681890
positive genetic interaction witherd1Erd1 homolog (predicted) Positive GeneticPMID:22681890
positive genetic interaction withmug20Schizosaccharomyces specific protein Mug20 Positive GeneticPMID:22681890
positive genetic interaction withadh4alcohol dehydrogenase Adh4 Positive GeneticPMID:22681890
positive genetic interaction withgmh4alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPAC23G3.05cregulator of G-protein signaling (RGS) domain (predicted) Positive GeneticPMID:22681890
positive genetic interaction withdbr1RNA lariat debranching enzyme Dbr1 Positive GeneticPMID:22681890
positive genetic interaction withabo2ATPase with bromodomain protein (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
positive genetic interaction withmod21gamma tubulin complex subunit Mod21 Positive GeneticPMID:22681890
positive genetic interaction withrud3Golgi matrix protein Rud3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withmug89CDC50 domain protein, implicated in signal transduction (predicted) Positive GeneticPMID:22681890
positive genetic interaction withbun107WD repeat protein, human WDR48 family Bun107 Positive GeneticPMID:22681890
positive genetic interaction withraf2Rik1-associated factor Raf2 Positive GeneticPMID:22681890
positive genetic interaction withSPAC144.05ATP-dependent DNA helicase/ ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withspa2cell polarity protein Spa2 Positive GeneticPMID:22681890
positive genetic interaction withspa1ornithine decarboxylase antizyme with +1 programmed ribosomal frameshift Spa1 Positive GeneticPMID:22681890
positive genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
positive genetic interaction withyip5Rab GTPase binding Yip5 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withgmh5alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:18818364
overexpression rescuespmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:12931193
overexpression rescuesrnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Dosage RescuePMID:12931193
overexpression rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:12931193
overexpression rescuesmkh1MEK kinase (MEKK) Mkh1 Dosage RescuePMID:10591634
synthetically rescuespck2protein kinase C (PKC)-like Pck2 Synthetic RescuePMID:17005909
synthetically rescuesrho2Rho family GTPase Rho2 Synthetic RescuePMID:17005909
synthetically rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic RescuePMID:17005909
synthetically rescueskin1microtubule affinity-regulating kinase Kin1 Synthetic RescuePMID:23294323
synthetic growth defect withmkh1MEK kinase (MEKK) Mkh1 Synthetic Growth DefectPMID:10365961
synthetic growth defect withppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic Growth DefectPMID:10365961
rescued by overexpression ofpmk1MAP kinase Pmk1 Dosage RescuePMID:10591634
rescued bypmk1MAP kinase Pmk1 Phenotypic SuppressionPMID:16928959
rescued byyam8stretch-activated calcium ion channel Yam8 Phenotypic SuppressionPMID:16928959
rescued bycch1calcium ion channel Cch1 Phenotypic SuppressionPMID:16928959
rescuescch1calcium ion channel Cch1 Phenotypic SuppressionPMID:21811607
External References
Database Identifier Description
NBRP SPBC543.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC543.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC543.07 BioGRID Interaction Datasets
Expression Viewer SPBC543.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC543.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC543.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC543.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC543.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC543.07 Transcriptome Viewer (Bähler Lab)
GEO SPBC543.07 GEO profiles
PInt SPBC543.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC543.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC543.07 Fission yeast phenotypic data & analysis
Cyclebase SPBC543.07.1 Cell Cycle Data
SPD / RIKEN32/32E04Orfeome Localization Data
UniProtKB/SwissProtQ9Y884MAP kinase kinase skh1/pek1
ModBaseQ9Y884Database of comparative protein structure models
STRINGQ9Y884Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596795MAP kinase kinase Pek1
RefSeq mRNANM_001023815972h- MAP kinase kinase Pek1 (pek1), mRNA
European Nucleotide ArchiveAAD41399.1ENA Protein Mapping
European Nucleotide ArchiveBAA82312.1ENA Protein Mapping
European Nucleotide ArchiveCAC05249.1ENA Protein Mapping
UniParcUPI00001359D9UniProt Archive

Literature for pek1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016