pek1 (SPBC543.07)


Gene Standard Namepek1 Characterisation Statuspublished
Systematic IDSPBC543.07 Feature Typeprotein coding
Synonymsmkk1, skh1 Name DescriptionPombe mEK 1
ProductMAP kinase kinase Pek1 Product Size363aa, 40.71 kDa
Genomic Location Chromosome II, 4312422-4314140 (1719nt); CDS:4312763-4313854 (1092nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0004708MAP kinase kinase activity3
has substrate pmk1IDAPMID:10365961
GO:0004674protein serine/threonine kinase activityIEAIPR008271GO_REF:0000002112
GO:0004713protein tyrosine kinase activity9
has substrate pmk1IDAPMID:10365961
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000165MAPK cascadeIDAPMID:1036596112
GO:0000187activation of MAPK activityIDAPMID:103659613
GO:0006883cellular sodium ion homeostasis5
different_pathwayNASGO_REF:0000001
GO:0071852fungal-type cell wall organization or biogenesisNASGO_REF:0000001100
GO:0000281mitotic cytokinesisNASGO_REF:000000182
GO:0018108peptidyl-tyrosine phosphorylationIDAPMID:103659618
GO:0010524positive regulation of calcium ion transport into cytosolIMPPMID:218116073
GO:0050850positive regulation of calcium-mediated signalingIMPPMID:169289596
GO:0008360regulation of cell shapeNASGO_REF:000000162
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030428cell septum27
during mitotic cytokinesisIDAPMID:16291757
GO:0005737cytoplasmIDAPMID:162917574204
GO:0005829cytosolIDAPMID:168233722319
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003535decreased bipolar indexMicroscopypek1ΔNullPECO:0000004PMID:2050195427
Microscopypek1ΔNullPMID:20501954
FYPO:0003532increased monopolar indexMicroscopypek1ΔNullPECO:0000005PMID:2050195435
Microscopypek1ΔNullPECO:0000004PMID:20501954
FYPO:0001192normal growth on cell wall-degrading enzymesCell growth assayPek1DD (S234D, T238D)Not specifiedPMID:103659618
FYPO:0001103resistance to hydrogen peroxide63
expressivity FYPO_EXT:0000001Cell growth assaypek1ΔNullPECO:0000137PMID:19672306
FYPO:0002642sensitive to amphotericin B69
expressivity FYPO_EXT:0000001Cell growth assaypek1ΔNullPECO:0000137, PECO:0000005PMID:23738021
FYPO:0000097sensitive to caffeine99
expressivity FYPO_EXT:0000001Cell growth assaypek1ΔNullPECO:0000137PMID:19672306
FYPO:0000085sensitive to camptothecincompetitive growth assay evidencepek1ΔNullPMID:20537132211
expressivity FYPO_EXT:0000003Cell growth assaypek1ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0001190sensitive to cell wall-degrading enzymesCell growth assaypek1ΔNullPMID:1036596141
Cell growth assayPek1A (T238A)Not specifiedPMID:10365961
FYPO:0000088sensitive to hydroxyurea513
expressivity FYPO_EXT:0000003Cell Growth Assaypek1ΔNullPECO:0000137, PECO:0000102PMID:23697806
FYPO:0002641sensitive to micafungin116
expressivity FYPO_EXT:0000001Cell growth assaypek1ΔNullPECO:0000137, PECO:0000005PMID:23738021
FYPO:0002617sensitive to sodium butyrate93
expressivity FYPO_EXT:0000001Cell growth assaypek1ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937
FYPO:0002546sensitive to trichostatin A39
expressivity FYPO_EXT:0000003Cell growth assaypek1ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937
FYPO:0000115sensitive to valproic acid155
expressivity FYPO_EXT:0000001Cell growth assaypek1ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937
FYPO:0002060viable vegetative cell populationCell growth assaypek1ΔNullPMID:103659613751
Microscopypek1ΔNullPMID:20473289
Microscopypek1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopypek1ΔNullPECO:0000004, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000032abnormal cytokinesisMicroscopypek1ΔNullPMID:1036596191
FYPO:0002288abolished protein tyrosine phosphorylation4
affecting pmk1Western blot assaypek1ΔNullPMID:10365961
FYPO:0001197decreased cellular calcium levelSubstance quantificationpek1ΔNullPECO:0000160, PECO:0000092PMID:218116076
FYPO:0001645decreased protein binding35
affecting pmk1 and pek1Co-immunoprecipitation experimentPek1DD (S234D, T238D)Not specifiedPMID:10365961
FYPO:0001122elongated vegetative cell663
penetrance FYPO_EXT:0000003Microscopypek1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001378increased cellular calcium level during cellular response to salt stressSubstance quantification evidencePek1DD (S234D, T238D)Not specifiedPMID:218116075
FYPO:0001571increased protein binding11
affecting pmk1 and pek1Co-immunoprecipitation experimentPek1A (T238A)Not specifiedPMID:10365961
FYPO:0001180increased protein tyrosine phosphorylation8
affecting pmk1Western blot assaypek1+ (wild type)OverexpressionPMID:10365961
affecting pmk1Western blot assayPek1DD (S234D, T238D)OverexpressionPMID:10365961
FYPO:0000647vegetative cell lysisCell growth assaypek1ΔNullPECO:0000137, PECO:0000005PMID:2373802179
FYPO:0001496viable elongated multiseptate vegetative cellMicroscopypek1ΔNullPMID:103659617
FYPO:0002106viable stubby vegetative cellMicroscopypek1ΔNullPECO:0000005, PECO:0000137PMID:2369780653
Microscopypek1ΔNullPECO:0000126PMID:10365961
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in pek1 MAP kinase kinase Pek1 PMID:10365961
GO substrate of mkh1 MEK kinase (MEKK) Mkh1 PMID:10365961
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
143124224314140
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 83 343 109
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 79 343 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 202 214 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 85 108 73
PS50011 Prosite Profiles IPR000719 Protein kinase domain 79 343 110
PTHR24360:SF16 HMMPANTHER 40 361 2
PTHR24360 HMMPANTHER 40 361 3
1.10.510.10 Gene3D 147 344 112
3.30.200.20 Gene3D 82 146 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 77 348 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.14 Da
Charge 7.00
Isoelectric point 8.20
Molecular weight 40.71 kDa
Number of residues 363
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2962during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2769during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2983during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3727during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3209during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1360.69during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.5during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.76during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
Mkh1-Pek1-Spm1 MAP kinase PMID:105916343
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
SPCC550.03cSki complex RNA helicase Ski2 (predicted) Negative GeneticPMID:22681890
rpp20160S acidic ribosomal protein A2 Negative GeneticPMID:22681890
mph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
ada1adenosine deaminase Ada1 Negative GeneticPMID:22681890
rpl430260S ribosomal protein L37a (predicted) Negative GeneticPMID:22681890
mga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Positive GeneticPMID:18818364
mpc1mitochondrial pyruvate transmembrane transporter subunit Mpc1 (predicted) Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
elp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
cdm1DNA polymerase delta subunit Cdm1 Positive GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
mpc2mitochondrial pyruvate transmembrane transporter Mpc2 (predicted) Negative GeneticPMID:22681890
ubp4ubiquitin C-terminal hydrolase Ubp4 Negative GeneticPMID:22681890
SPBPB7E8.01Schizosaccharomyces specific protein, predicted GPI anchor Negative GeneticPMID:22681890
tma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Synthetic RescuePMID:23294323
Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:18818364
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
gmh4alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:22681890
idp1isocitrate dehydrogenase Idp1 (predicted) Negative GeneticPMID:22681890
SPAC750.08cNAD-dependent malic enzyme (predicted), partial Negative GeneticPMID:22681890
emc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
swi6HP1 family chromodomain protein Swi6 Positive GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
ams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
gpa2heterotrimeric G protein alpha-2 subunit Gpa2 Negative GeneticPMID:22681890
mug82mitochondrial translation release factor (predicted) Negative GeneticPMID:22681890
caf1CCR4-Not complex CAF1 family ribonuclease subunit Caf1 Negative GeneticPMID:18818364
mso1endocytic docking protein Mso1 Negative GeneticPMID:22681890
csn3COP9/signalosome complex subunit Csn3 (predicted) Positive GeneticPMID:22681890
zds1zds family protein phosphatase type A regulator Zds1 (predicted) Negative GeneticPMID:18818364
ulp1SUMO deconjugating enzyme Ulp1 Negative GeneticPMID:22681890
SPAC227.06Rab GTPase binding (predicted) Positive GeneticPMID:22681890
raf2Rik1-associated factor Raf2 Positive GeneticPMID:22681890
moe1translation initiation factor eIF3d Moe1 Negative GeneticPMID:22681890
gmh5alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:18818364
SPBC839.02arrestin Aly1 related, implicated in endocytosis Negative GeneticPMID:22681890
mug80cyclin Clg1 (predicted) Negative GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
adh4alcohol dehydrogenase Adh4 Positive GeneticPMID:22681890
psy2protein phosphatase PP4 complex subunit Psy2 (predicted) Negative GeneticPMID:22681890
int6eIF3e subunit Int6 Negative GeneticPMID:22681890
pck2protein kinase C (PKC)-like Pck2 Synthetic RescuePMID:17005909
dbr1RNA lariat debranching enzyme Dbr1 Positive GeneticPMID:22681890
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:12931193
Synthetic RescuePMID:17005909
Synthetic RescuePMID:10365961
Dosage Rescue
Synthetic Growth Defect
srb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
SPCC622.03cdubious Negative GeneticPMID:22681890
ppm1leucine carboxyl methyltransferase Ppm1 (predicted) Positive GeneticPMID:22681890
mkh1MEK kinase (MEKK) Mkh1 Dosage RescuePMID:10591634
Synthetic Growth DefectPMID:10365961
SPAC19A8.11crecombination protein Irc6 (predicted) Negative GeneticPMID:22681890
rnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Dosage RescuePMID:12931193
SPAC23G3.05cregulator of G-protein signaling (RGS) domain (predicted) Positive GeneticPMID:22681890
ase1antiparallel microtubule cross-linking factor Ase1 Positive GeneticPMID:18818364
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
snz1pyridoxine biosynthesis protein Positive GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:18818364
Positive GeneticPMID:22681890
ftp105Ubp5 interacting protein Ftp105 Negative GeneticPMID:22681890
tsc1hamartin Negative GeneticPMID:22681890
cwf14G10 protein Positive GeneticPMID:22681890
rok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:22681890
SPCC285.05purine nucleoside transmembrane transporter (predicted) Negative GeneticPMID:22681890
SPAC9G1.07Schizosaccharomyces specific protein Positive GeneticPMID:22681890
mug89CDC50 domain protein, implicated in signal transduction (predicted) Positive GeneticPMID:22681890
not2CCR4-Not complex subunit Not2 (predicted) Negative GeneticPMID:18818364
ppr5mitochondrial PPR repeat protein Ppr5 Negative GeneticPMID:22681890
mug20Schizosaccharomyces specific protein Positive GeneticPMID:22681890
erg5C-22 sterol desaturase Erg5 Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
mod21gamma tubulin complex subunit Mod21 Positive GeneticPMID:22681890
erg28Erg28 protein (predicted) Negative GeneticPMID:22681890
SPBC365.16conserved protein Negative GeneticPMID:22681890
abo2ATPase with bromodomain protein (predicted) Positive GeneticPMID:22681890
SPAC17H9.08mitochondrial coenzyme A transporter (predicted) Negative GeneticPMID:22681890
rud3Golgi matrix protein Rud3 (predicted) Positive GeneticPMID:22681890
swd2Set1C complex subunit Swd2.1 Positive GeneticPMID:18818364
scs7sphingosine hydroxylase Scs7 Negative GeneticPMID:18818364
dad1DASH complex subunit Dad1 Positive GeneticPMID:22681890
pmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:12931193
ubp3ubiquitin C-terminal hydrolase Ubp3 Negative GeneticPMID:18818364
nmt14-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase Nmt1 Negative GeneticPMID:22681890
dcr1dicer Positive GeneticPMID:18818364
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
tcg1single-stranded telomeric binding protein Tgc1 Negative GeneticPMID:22681890
SPAC4A8.14ribose-phosphate pyrophosphokinase (predicted) Negative GeneticPMID:22681890
erd1Erd1 homolog (predicted) Positive GeneticPMID:22681890
rho2Rho family GTPase Rho2 Synthetic RescuePMID:17005909
SPBC725.01aspartate aminotransferase (predicted) Negative GeneticPMID:22681890
pmk1MAP kinase Pmk1 Dosage RescuePMID:10591634
msy1MS calcium ion channel protein Msy1 Negative GeneticPMID:22681890
SPAC144.05ATP-dependent DNA helicase/ ubiquitin ligase E3 (predicted) Positive GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
mug122PX/PXA domain protein Negative GeneticPMID:22681890
arp8actin-like protein, Ino80 complex subunit Arp8 Positive GeneticPMID:22681890
SPAC694.02DEAD/DEAH box helicase Negative GeneticPMID:22681890
apt1adenine phosphoribosyltransferase (APRT) (predicted) Negative GeneticPMID:22681890
SPBC1348.03S. pombe specific 5Tm protein family Negative GeneticPMID:22681890
spa2GTPase activating protein Spa2 (predicted) Positive GeneticPMID:22681890
bun107WD repeat protein, human WDR48 family Bun107 Positive GeneticPMID:22681890
spa1ornithine decarboxylase antizyme with +1 programmed ribosomal frameshift Spa1 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
pmk1MAP kinase Pmk1 Biochemical ActivityPMID:10591634
Affinity Capture-WesternPMID:16291757
Affinity Capture-WesternPMID:10365961
mkh1MEK kinase (MEKK) Mkh1 Two-hybridPMID:10591634
Two-hybridPMID:23695164
hsp16heat shock protein Hsp16 Two-hybridPMID:23695164
External References
Database Identifier Description
NBRP SPBC543.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC543.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC543.07 BioGRID Interaction Datasets
Expression Viewer SPBC543.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC543.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC543.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC543.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC543.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC543.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC543.07 Cell Cycle Data
GEO SPBC543.07 GEO profiles
PInt SPBC543.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC543.07 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN32/32E04Orfeome Localization Data
UniProtKB/SwissProtQ9Y884MAP kinase kinase skh1/pek1
ModBaseQ9Y884Database of comparative protein structure models
STRINGQ9Y884Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596795MAP kinase kinase Pek1
RefSeq mRNANM_001023815972h- MAP kinase kinase Pek1 (pek1), mRNA
European Nucleotide ArchiveAAD41399.1ENA Protein Mapping
European Nucleotide ArchiveBAA82312.1ENA Protein Mapping
European Nucleotide ArchiveCAC05249.1ENA Protein Mapping
UniParcUPI00001359D9UniProt Archive

Literature for pek1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014