pek1 (SPBC543.07)

Gene Standard Namepek1 Characterisation Statuspublished
Systematic IDSPBC543.07 Feature Typeprotein coding
Synonymsmkk1, skh1 Name DescriptionPombe mEK 1
ProductMAP kinase kinase Pek1 Product Size363aa, 40.71 kDa
Genomic Location Chromosome II, 4312422-4314140 (1719nt); CDS:4312763-4313854 (1092nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
MAP kinase kinase activity3
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine kinase activity112
Annotation ExtensionEvidenceWith/FromReference
protein tyrosine kinase activity9
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
activation of MAPK activity3
Annotation ExtensionEvidenceWith/FromReference
cellular sodium ion homeostasis5
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall organization or biogenesis120
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade25
Annotation ExtensionEvidenceWith/FromReference
mitotic cytokinesis134
Annotation ExtensionEvidenceWith/FromReference
peptidyl-tyrosine phosphorylation11
Annotation ExtensionEvidenceWith/FromReference
positive regulation of calcium ion transport into cytosol3
Annotation ExtensionEvidenceWith/FromReference
positive regulation of calcium-mediated signaling6
Annotation ExtensionEvidenceWith/FromReference
regulation of cell shape77
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell septum27
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased bipolar indexpek1ΔNull27
increased monopolar indexpek1ΔNull35
normal growth on cell wall-degrading enzymespek1DD (S234D, T238D)Not specified8
resistance to hydrogen peroxide72
expressivity FYPO_EXT:0000001pek1ΔNull
sensitive to amphotericin B69
expressivity FYPO_EXT:0000001pek1ΔNull
sensitive to caffeine108
expressivity FYPO_EXT:0000001pek1ΔNull
sensitive to camptothecinpek1ΔNull214
expressivity FYPO_EXT:0000003pek1ΔNull
sensitive to cell wall-degrading enzymespek1ΔNull42
pek1A (T238A)Not specified
sensitive to hydroxyurea518
expressivity FYPO_EXT:0000003pek1ΔNull
sensitive to micafungin116
expressivity FYPO_EXT:0000001pek1ΔNull
sensitive to sodium butyrate93
expressivity FYPO_EXT:0000001pek1ΔNull
sensitive to trichostatin A42
expressivity FYPO_EXT:0000003pek1ΔNull
sensitive to valproic acid155
expressivity FYPO_EXT:0000001pek1ΔNull
viable vegetative cell populationpek1ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
abnormal cytokinesispek1ΔNull182
abolished protein threonine phosphorylation at Thr-Pro site2
affecting rnc1pek1A (T238A)Not specified
abolished protein tyrosine phosphorylation4
affecting pmk1pek1ΔNull
abolished RNA binding10
affecting pmp1 and rnc1pek1A (T238A)Not specified
decreased cellular calcium levelpek1ΔNull6
decreased protein binding51
affecting pmk1 and pek1pek1DD (S234D, T238D)Not specified
decreased RNA catabolic process during vegetative growth10
affecting pmp1pek1DD (S234D, T238D)Not specified
elongated vegetative cell703
penetrance FYPO_EXT:0000003pek1ΔNull
increased cellular calcium level during cellular response to salt stresspek1DD (S234D, T238D)Not specified5
increased protein binding11
affecting pmk1 and pek1pek1A (T238A)Not specified
increased protein threonine phosphorylation at Thr-Pro site1
affecting rnc1pek1DD (S234D, T238D)Not specified
increased protein tyrosine phosphorylation10
affecting pmk1pek1+ (wild type)Overexpression
affecting pmk1pek1DD (S234D, T238D)Overexpression
increased RNA binding1
affecting pmp1 and rnc1pek1DD (S234D, T238D)Not specified
increased RNA catabolic process during vegetative growth4
affecting pmp1pek1A (T238A)Not specified
vegetative cell lysispek1ΔNull82
viable elongated multiseptate vegetative cellpek1ΔNull8
viable stubby vegetative cellpek1ΔNull70
Target Of
FYPO affected by mutation in pek1 MAP kinase kinase Pek1 PMID:10365961
GO substrate of mkh1 MEK kinase (MEKK) Mkh1 PMID:10365961
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 83 343 109
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 79 343 107
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 85 108 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 202 214 97
PS50011 Prosite Profiles IPR000719 Protein kinase domain 79 343 110
PTHR24360 HMMPANTHER 62 361 3
PTHR24360:SF45 HMMPANTHER 62 361 1
1.10.510.10 Gene3D 147 344 112 Gene3D 82 146 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 77 348 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.14 Da
Charge 7.00
Isoelectric point 8.20
Molecular weight 40.71 kDa
Number of residues 363
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2962during GO:0000080PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
2769during GO:0000084PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
2983during GO:0000085PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
3727during GO:0000087PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
1360.69during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
3209during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.5during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
0.76during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
Mkh1-Pek1-Spm1 MAP kinase PMID:105916343
Species Distribution
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
pmk1MAP kinase Pmk1 Biochemical ActivityPMID:10591634
Affinity Capture-WesternPMID:16291757
Affinity Capture-WesternPMID:10365961
mkh1MEK kinase (MEKK) Mkh1 Two-hybridPMID:10591634
hsp16heat shock protein Hsp16 Two-hybridPMID:23695164
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
SPCC550.03cSki complex RNA helicase Ski2 (predicted) Negative GeneticPMID:22681890
rpp20160S acidic ribosomal protein A2 Negative GeneticPMID:22681890
mph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
ada1adenosine deaminase Ada1 Negative GeneticPMID:22681890
rpl430260S ribosomal protein L37a (predicted) Negative GeneticPMID:22681890
mga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Positive GeneticPMID:18818364
mpc1mitochondrial pyruvate transmembrane transporter subunit Mpc1 (predicted) Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
elp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
cdm1DNA polymerase delta subunit Cdm1 Positive GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
mpc2mitochondrial pyruvate transmembrane transporter Mpc2 (predicted) Negative GeneticPMID:22681890
ubp4ubiquitin C-terminal hydrolase Ubp4 Negative GeneticPMID:22681890
SPBPB7E8.01Schizosaccharomyces specific protein, predicted GPI anchor Negative GeneticPMID:22681890
tma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Synthetic RescuePMID:23294323
Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:18818364
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
gmh4alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:22681890
idp1isocitrate dehydrogenase Idp1 (predicted) Negative GeneticPMID:22681890
SPAC750.08cNAD-dependent malic enzyme (predicted), partial Negative GeneticPMID:22681890
emc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
swi6HP1 family chromodomain protein Swi6 Positive GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
ams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
gpa2heterotrimeric G protein alpha-2 subunit Gpa2 Negative GeneticPMID:22681890
pth4mitochondrial translation release factor Negative GeneticPMID:22681890
caf1CCR4-Not complex CAF1 family ribonuclease subunit Caf1 Negative GeneticPMID:18818364
mso1endocytic docking protein Mso1 Negative GeneticPMID:22681890
csn3COP9/signalosome complex subunit Csn3 (predicted) Positive GeneticPMID:22681890
zds1zds family protein phosphatase type A regulator Zds1 (predicted) Negative GeneticPMID:18818364
ulp1SUMO deconjugating enzyme Ulp1 Negative GeneticPMID:22681890
yip5Rab GTPase binding Yip5 (predicted) Positive GeneticPMID:22681890
raf2Rik1-associated factor Raf2 Positive GeneticPMID:22681890
moe1translation initiation factor eIF3d Moe1 Negative GeneticPMID:22681890
gmh5alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:18818364
SPBC839.02arrestin Aly1 related, implicated in endocytosis Negative GeneticPMID:22681890
mug80cyclin Clg1 (predicted) Negative GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
adh4alcohol dehydrogenase Adh4 Positive GeneticPMID:22681890
psy2protein phosphatase PP4 complex subunit Psy2 (predicted) Negative GeneticPMID:22681890
int6eIF3e subunit Int6 Negative GeneticPMID:22681890
pck2protein kinase C (PKC)-like Pck2 Synthetic RescuePMID:17005909
dbr1RNA lariat debranching enzyme Dbr1 Positive GeneticPMID:22681890
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:12931193
Synthetic RescuePMID:17005909
Synthetic Growth DefectPMID:10365961
Synthetic Rescue
Dosage Rescue
srb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
SPCC622.03cdubious Negative GeneticPMID:22681890
ppm1leucine carboxyl methyltransferase Ppm1 (predicted) Positive GeneticPMID:22681890
mkh1MEK kinase (MEKK) Mkh1 Dosage RescuePMID:10591634
Synthetic Growth DefectPMID:10365961
SPAC19A8.11crecombination protein Irc6 (predicted) Negative GeneticPMID:22681890
rnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Dosage RescuePMID:12931193
SPAC23G3.05cregulator of G-protein signaling (RGS) domain (predicted) Positive GeneticPMID:22681890
ase1antiparallel microtubule cross-linking factor Ase1 Positive GeneticPMID:18818364
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
snz1pyridoxine biosynthesis protein Positive GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:18818364
Positive GeneticPMID:22681890
ftp105Ubp5 interacting protein Ftp105 Negative GeneticPMID:22681890
tsc1hamartin Negative GeneticPMID:22681890
cwf14G10 protein Positive GeneticPMID:22681890
rok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:22681890
SPCC285.05purine nucleoside transmembrane transporter (predicted) Negative GeneticPMID:22681890
SPAC9G1.07Schizosaccharomyces specific protein Positive GeneticPMID:22681890
mug89CDC50 domain protein, implicated in signal transduction (predicted) Positive GeneticPMID:22681890
not2CCR4-Not complex subunit Not2 (predicted) Negative GeneticPMID:18818364
ppr5mitochondrial PPR repeat protein Ppr5 Negative GeneticPMID:22681890
mug20Schizosaccharomyces specific protein Mug20 Positive GeneticPMID:22681890
erg5C-22 sterol desaturase Erg5 Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
mod21gamma tubulin complex subunit Mod21 Positive GeneticPMID:22681890
erg28Erg28 protein (predicted) Negative GeneticPMID:22681890
SPBC365.16conserved protein Negative GeneticPMID:22681890
abo2ATPase with bromodomain protein (predicted) Positive GeneticPMID:22681890
SPAC17H9.08mitochondrial coenzyme A transmembrane transporter (predicted) Negative GeneticPMID:22681890
rud3Golgi matrix protein Rud3 (predicted) Positive GeneticPMID:22681890
swd2Set1C complex subunit Swd2.1 Positive GeneticPMID:18818364
scs7sphingosine hydroxylase Scs7 Negative GeneticPMID:18818364
dad1DASH complex subunit Dad1 Positive GeneticPMID:22681890
pmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:12931193
ubp3ubiquitin C-terminal hydrolase Ubp3 Negative GeneticPMID:18818364
nmt14-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase Nmt1 Negative GeneticPMID:22681890
dcr1dicer Positive GeneticPMID:18818364
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
tcg1single-stranded telomeric binding protein Tgc1 Negative GeneticPMID:22681890
SPAC4A8.14ribose-phosphate pyrophosphokinase (predicted) Negative GeneticPMID:22681890
erd1Erd1 homolog (predicted) Positive GeneticPMID:22681890
rho2Rho family GTPase Rho2 Synthetic RescuePMID:17005909
SPBC725.01aspartate aminotransferase (predicted) Negative GeneticPMID:22681890
pmk1MAP kinase Pmk1 Dosage RescuePMID:10591634
msy1MS calcium ion channel protein Msy1 Negative GeneticPMID:22681890
SPAC144.05ATP-dependent DNA helicase/ ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
mug122PX/PXA domain protein Negative GeneticPMID:22681890
arp8actin-like protein, Ino80 complex subunit Arp8 Positive GeneticPMID:22681890
SPAC694.02DEAD/DEAH box helicase Negative GeneticPMID:22681890
apt1adenine phosphoribosyltransferase (APRT) (predicted) Negative GeneticPMID:22681890
SPBC1348.03S. pombe specific 5Tm protein family Negative GeneticPMID:22681890
spa2GTPase activating protein Spa2 (predicted) Positive GeneticPMID:22681890
bun107WD repeat protein, human WDR48 family Bun107 Positive GeneticPMID:22681890
spa1ornithine decarboxylase antizyme with +1 programmed ribosomal frameshift Spa1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC543.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC543.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC543.07 BioGRID Interaction Datasets
Expression Viewer SPBC543.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC543.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC543.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC543.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC543.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC543.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC543.07 Cell Cycle Data
GEO SPBC543.07 GEO profiles
PInt SPBC543.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC543.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC543.07 Fission yeast phenotypic data & analysis
SPD / RIKEN32/32E04Orfeome Localization Data
UniProtKB/SwissProtQ9Y884MAP kinase kinase skh1/pek1
ModBaseQ9Y884Database of comparative protein structure models
STRINGQ9Y884Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596795MAP kinase kinase Pek1
RefSeq mRNANM_001023815972h- MAP kinase kinase Pek1 (pek1), mRNA
European Nucleotide ArchiveAAD41399.1ENA Protein Mapping
European Nucleotide ArchiveBAA82312.1ENA Protein Mapping
European Nucleotide ArchiveCAC05249.1ENA Protein Mapping
UniParcUPI00001359D9UniProt Archive

Literature for pek1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015