SPBC582.10c


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPBC582.10c Feature Typeprotein coding
Synonyms Name Description
ProductATP-dependent DNA helicase Rhp16b (predicted) Product Size830aa, 93.95 kDa
Genomic Location Chromosome II, 439017-436090 (2928nt); CDS:438777-436233 (2545nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
DNA binding379
Annotation ExtensionEvidenceWith/FromReference
DNA-dependent ATPase activity65
Annotation ExtensionEvidenceWith/FromReference
helicase activity94
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

DNA repair


Term NameCount
nucleotide-excision repair28
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleotide-excision repair factor 4 complex1
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationSPBC582.10cΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologySPBC582.10cΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons439017..437989, 437936..436090
mRNA439017..436090
5' UTR439017..438778PMID:21511999
CDS438777..437989, 437936..436233
3' UTR436232..436090PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00271 Pfam IPR001650 Helicase, C-terminal 689 767 68
PF00176 Pfam IPR000330 SNF2-related 236 554 20
SM00487 SMART IPR014001 Helicase superfamily 1/2, ATP-binding domain 229 445 69
SM00490 SMART IPR001650 Helicase, C-terminal 684 767 68
PS51192 Prosite Profiles IPR014001 Helicase superfamily 1/2, ATP-binding domain 249 433 69
PS51194 Prosite Profiles IPR001650 Helicase, C-terminal 662 816 69
PTHR10799 HMMPANTHER 1 829 20
PTHR10799:SF232 HMMPANTHER 1 829 2
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 646 799 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 230 432 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 210 472 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 639 827 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 491 573 294
Coil ncoils Rabaptin coiled-coil domain 780 801 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174
PBO:0000175helicase C-terminal domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000175

Protein Properties

Ave. residue weight 113.19 Da
Charge 40.00
Isoelectric point 9.91
Molecular weight 93.95 kDa
Number of residues 830
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS812
present during cellular response to thiabendazoleS712
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S712 PMID:18257517
present during mitotic M phase experimental evidence S812 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1280during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1129during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1233during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1442during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1279during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.73during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.23during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withalg12dolichyl pyrophosphate Man7GlcNAc2 alpha-1,6-mannosyltransferase Alg12 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withalm1medial ring protein Alm1 Negative GeneticPMID:22681890
positive genetic interaction withcgs2cAMP-specific phosphodiesterase Cgs2 Positive GeneticPMID:22681890
positive genetic interaction withcia1histone chaperone Cia1 Positive GeneticPMID:18818364
positive genetic interaction withcid12poly(A) polymerase Cid12 Positive GeneticPMID:18818364
positive genetic interaction withdep1Sds3-like family protein Dep1 Positive GeneticPMID:22681890
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
positive genetic interaction withgar2nucleolar protein required for rRNA processing Positive GeneticPMID:22681890
positive genetic interaction withhmt2sulfide-quinone oxidoreductase Positive GeneticPMID:22681890
positive genetic interaction withmal3EB1 family Mal3 Positive GeneticPMID:22681890
positive genetic interaction withmeu10GPI anchored cell surface protein involved in ascospore wall assembly Meu10 Positive GeneticPMID:22681890
negative genetic interaction withSPAP8A3.07cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:18818364
positive genetic interaction withSPBC31F10.02acyl-CoA hydrolase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPBC725.10mitochondrial transport protein, tspO homolog (predicted) Positive GeneticPMID:22681890
negative genetic interaction withspp27RNA polymerase I upstream activation factor complex subunit Spp27 Negative GeneticPMID:22681890
positive genetic interaction withste20Rictor homolog, Ste20 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC582.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC582.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC582.10c BioGRID Interaction Datasets
Expression Viewer SPBC582.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC582.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC582.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC582.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC582.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC582.10c Transcriptome Viewer (Bähler Lab)
GEO SPBC582.10c GEO profiles
PInt SPBC582.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC582.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC582.10c Fission yeast phenotypic data & analysis
Cyclebase SPBC582.10c.1 Cell Cycle Data
SPD / RIKEN29/29C11Orfeome Localization Data
UniProtKB/SwissProtQ10332Uncharacterized ATP-dependent helicase C582.10c
ModBaseQ10332Database of comparative protein structure models
STRINGQ10332Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595178ATP-dependent DNA helicase Rhp16b (predicted)
RefSeq mRNANM_001021086972h- ATP-dependent DNA helicase Rhp16b (predicted) (SPBC582.10c), mRNA
European Nucleotide ArchiveBAA87269.1ENA Protein Mapping
European Nucleotide ArchiveCAB46673.1ENA Protein Mapping
UniParcUPI000013A3B6UniProt Archive

Literature for SPBC582.10c

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015