ntp1 (SPBC660.07)


Gene Standard Namentp1 Characterisation Statuspublished
Systematic IDSPBC660.07 Feature Typeprotein coding
Synonyms Name Description
Productalpha,alpha-trehalase Ntp1 Product Size735aa, 84.60 kDa
Genomic Location Chromosome II, 206580-209641 (3062nt); CDS:207078-209285 (2208nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004555alpha,alpha-trehalase activityIMPPMID:159656431
during cellular response to glucose stimulusIMPPMID:9495778
IDAPMID:12153582
during cellular response to heatIMPPMID:9495778
IMPPMID:10223994
GO:0005509calcium ion binding52
residue 99|108IDAPMID:12943532
GO:0005515protein bindingIPItpp1PMID:12383265861
IPIntp1PMID:12153582
IPItps1PMID:12383265
IPItpp1PMID:12153582
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030437ascospore formationIMPPMID:1109428987
GO:0005993trehalose catabolic processIMPPMID:102239941
IMPPMID:9729425
during cellular response to oxidative stressIMPPMID:9974219
GO:1903134trehalose catabolic process involved in cellular response to stressIMPPMID:159656431
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001454normal during cellular response to freezingCell growth assayntp1ΔNullPECO:0000125, PECO:0000126, PECO:0000102PMID:102239942
FYPO:0000960normal growth on ethanolCell growth assayntp1ΔNullPECO:0000126, PECO:0000102PMID:102239942
FYPO:0001420normal vegetative cell population growth rateCell growth assayntp1ΔNullPECO:0000126PMID:949577838
Cell growth assayntp1ΔNullPECO:0000137PMID:9495778
FYPO:0001090resistance to heat shock during vegetative growthCell growth assayntp1ΔNullPECO:0000168PMID:94957786
Cell growth assayntp1ΔNullPECO:0000103, PECO:0000126, PECO:0000117PMID:10223994
FYPO:0000852resistance to salt stressCell growth assayntp1ΔNullPECO:0000102, PECO:0000166, PECO:0000137PMID:94957785
Cell growth assayntp1ΔNullPECO:0000103, PECO:0000137, PECO:0000166PMID:9495778
FYPO:0001452resistance to water deprivationCell growth assayntp1ΔNullPECO:0000125, PECO:0000126, PECO:0000102PMID:102239942
FYPO:0002684sensitive to phlorizin during spore germinationCell growth assayntp1ΔNullPMID:110942891
FYPO:0002060viable vegetative cell populationMicroscopyntp1ΔNullPMID:204732893760
Microscopyntp1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003463abolished alpha,alpha-trehalase activityEnzyme assay dataS51A (S51A)Not specifiedPMID:159656431
Enzyme assay dataS6A (S6A)Not specifiedPMID:15965643
Enzyme assay dataS71A (S71A)Not specifiedPMID:15965643
FYPO:0001482alpha,alpha-trehalase activity increase abolished during cellular response to heatEnzyme assay datantp1ΔNullPMID:94957782
Enzyme assay dataS71D (S71D)Not specifiedPMID:15965643
Enzyme assay dataS51A (S51A)Not specifiedPMID:15965643
Enzyme assay dataS6A (S6A)Not specifiedPMID:15965643
Enzyme assay dataS71A (S71A)Not specifiedPMID:15965643
FYPO:0001481alpha,alpha-trehalase activity increase abolished during cellular response to nutrientEnzyme assay datantp1ΔNullPMID:94957783
FYPO:0003458alpha,alpha-trehalase activity increase abolished during cellular response to salt stressEnzyme assay dataS51A (S51A)Not specifiedPECO:0000166PMID:159656432
Enzyme assay dataS71D (S71D)Not specifiedPECO:0000166PMID:15965643
Enzyme assay dataS71A (S71A)Not specifiedPECO:0000166PMID:15965643
Enzyme assay dataS6A (S6A)Not specifiedPECO:0000166PMID:15965643
FYPO:0001116decreased RNA level during cellular response to hydrogen peroxide11
affecting ntp1Transcript expression level evidenceCRE-like promoter mutant (TGACGTAG mutated to TGACcTAG)Not specifiedPMID:12855726
FYPO:0002304decreased RNA level during cellular response to salt stress8
affecting ntp1Transcript expression level evidenceCRE-like promoter mutant (TGACGTAG mutated to TGACcTAG)Not specifiedPECO:0000166PMID:12855726
FYPO:0003464decreased alpha,alpha-trehalase activity during cellular response to heatEnzyme assay dataS41A (S41A)Not specifiedPMID:159656431
FYPO:0003465decreased alpha,alpha-trehalase activity during cellular response to salt stressEnzyme assay dataS41A (S41A)Not specifiedPECO:0000166PMID:159656431
FYPO:0000581decreased spore germinationCell growth assayntp1ΔNullPECO:0000103PMID:1109428914
FYPO:0001502increased cellular trehalose level during cellular response to osmotic stressSubstance quantification evidenceS51A (S51A)Not specifiedPECO:0000166PMID:159656431
Substance quantification evidencentp1ΔNullPECO:0000166PMID:15965643
Substance quantification evidenceS41A (S41A)Not specifiedPECO:0000166PMID:15965643
Substance quantification evidenceS71D (S71D)Not specifiedPECO:0000166PMID:15965643
Enzyme assay datantp1ΔNullPECO:0000137, PECO:0000166PMID:9495778
Substance quantification evidenceS6A (S6A)Not specifiedPECO:0000166PMID:15965643
Substance quantification evidenceS71A (S71A)Not specifiedPECO:0000166PMID:15965643
FYPO:0001445increased cellular trehalose level during heat shockSubstance quantification evidenceS71D (S71D)Not specifiedPMID:159656433
Substance quantification evidenceS71A (S71A)Not specifiedPMID:15965643
Enzyme assay datantp1ΔNullPMID:9495778
Enzyme assay datantp1ΔNullPECO:0000125, PECO:0000126, PECO:0000117PMID:10223994
Substance quantification evidencentp1ΔNullPMID:15965643
FYPO:0001444increased cellular trehalose level during vegetative growthSubstance quantification evidencentp1ΔNullPMID:159656433
Substance quantification evidenceS51A (S51A)Not specifiedPMID:15965643
Substance quantification evidenceS71A (S71A)Not specifiedPMID:15965643
Substance quantification evidenceS6A (S6A)Not specifiedPMID:15965643
Enzyme assay datantp1ΔNullPECO:0000137PMID:9495778
Substance quantification evidencentp1ΔNullPMID:9729425
Substance quantification evidenceS71D (S71D)Not specifiedPMID:15965643
Enzyme assay datantp1ΔNullPECO:0000126, PECO:0000125PMID:10223994
FYPO:0002686increased cellular trehalose level in sporeCell growth assayntp1ΔNullPMID:110942891
FYPO:0001480normal alpha,alpha-trehalase activityEnzyme assay dataI104A (I104A)Not specifiedPMID:159656436
Enzyme assay dataS41A (S41A)Not specifiedPMID:15965643
FYPO:0003265normal alpha,alpha-trehalase activity increase during cellular response to heat stressEnzyme assay dataI104A (I104A)Not specifiedPMID:159656433
FYPO:0001682normal alpha,alpha-trehalase activity increase during cellular response to salt stressEnzyme assay dataI104A (I104A)Not specifiedPECO:0000166PMID:159656434
FYPO:0001091normal cellular response to heat shockCell growth assayntp1ΔNullPECO:0000004PMID:94957782
FYPO:0001449normal cellular trehalose levelSubstance quantification evidenceS41A (S41A)Not specifiedPMID:159656434
Substance quantification evidenceI104A (I104A)Not specifiedPMID:15965643
FYPO:0003466normal cellular trehalose level during cellular response to heatSubstance quantification evidenceS6A (S6A)Not specifiedPMID:159656431
Substance quantification evidenceI104A (I104A)Not specifiedPMID:15965643
FYPO:0003461normal cellular trehalose level during cellular response to salt stressSubstance quantification evidenceI104A (I104A)Not specifiedPECO:0000166PMID:159656433
FYPO:0001147normal mating efficiencyCell growth assayntp1ΔNullPMID:949577816
FYPO:0000703normal protein binding30
affecting tps1
affecting ntp1
Co-immunoprecipitation experimentS6A (S6A)Not specifiedPMID:15965643
affecting tps1
affecting ntp1
Co-immunoprecipitation experimentS51A (S51A)Not specifiedPMID:15965643
affecting tps1
affecting ntp1
Co-immunoprecipitation experimentS71A (S71A)Not specifiedPMID:15965643
affecting tps1
affecting ntp1
Co-immunoprecipitation experimentS41A (S41A)Not specifiedPMID:15965643
affecting tps1
affecting ntp1
Co-immunoprecipitation experimentS71D (S71D)Not specifiedPMID:15965643
FYPO:0000579normal spore germinationCell growth assayntp1ΔNullPMID:94957788
FYPO:0001315normal vegetative cell morphologyMicroscopyntp1ΔNullPMID:949577870
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopyntp1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1 PMID:12855726
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1 PMID:9974219
FYPO affected by mutation in mcs4 response regulator Mcs4 PMID:9974219
FYPO affected by mutation in ntp1 alpha,alpha-trehalase Ntp1 PMID:15965643
FYPO affected by mutation in ntp1 alpha,alpha-trehalase Ntp1 PMID:12855726
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:12855726
FYPO affected by mutation in pcr1 transcription factor Pcr1 PMID:12855726
FYPO affected by mutation in sty1 MAP kinase Sty1 PMID:12855726
FYPO affected by mutation in sty1 MAP kinase Sty1 PMID:9974219
FYPO affected by mutation in tps1 alpha,alpha-trehalose-phosphate synthase [UDP-forming] PMID:9729425
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:9974219
FYPO affected by mutation in wis4 MAP kinase kinase kinase Wis4 PMID:9974219
GO regulated by atf1 transcription factor, Atf-CREB family Atf1 PMID:15164362
GO regulated by pcr1 transcription factor Pcr1 PMID:15164362
GO regulated by sty1 MAP kinase Sty1 PMID:15164362
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1206580209641

UTRs

Region Coordinates Reference
five_prime_UTR206580..207077PMID:21511999
three_prime_UTR209286..209641PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01204 Pfam IPR001661 Glycoside hydrolase, family 37 146 699 1
PF07492 Pfam IPR011120 Neutral trehalase Ca2+ binding 89 118 1
PS00927 Prosite Patterns IPR018232 Glycoside hydrolase, family 37, conserved site 280 293 1
PS00928 Prosite Patterns IPR018232 Glycoside hydrolase, family 37, conserved site 601 610 1
PTHR23403:SF6 HMMPANTHER 41 731 1
PTHR23403 HMMPANTHER IPR001661 Glycoside hydrolase, family 37 41 731 1
SSF48208 SuperFamily IPR008928 Six-hairpin glycosidase-like 173 695 9
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 594 610 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 521 537 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 469 486 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 322 340 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 682 695 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 390 407 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 445 462 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 115.11 Da
Charge -5.00
Isoelectric point 6.03
Molecular weight 84.60 kDa
Number of residues 735
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS731PMID:247631071663
present during mitotic M phaseS731PMID:21712547
S16PMID:24763107
present during mitotic M phaseS16PMID:21712547
MOD:00047O-phospho-L-threonineT25PMID:24763107682
MOD:00696phosphorylated residueIDAPMID:99742191915
added during cellular response to oxidative stress, required for alpha,alpha-trehalase activity
NASPMID:19547744
NASPMID:15965654
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0070301Northern assay evidencePMID:12855726
increased during GO:0071472Northern assay evidencePMID:12855726
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased during GO:0034605expression microarray evidencePMID:12529438
increased during GO:0034599expression microarray evidencePMID:12529438
increased during GO:0034605Northern assay evidencePMID:9729425
increased during GO:0071470expression microarray evidencePMID:12529438
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0071472Northern assay evidencePMID:9729425
present during GO:0072690Northern assay evidencePMID:9495778
increased during GO:0034605Northern assay evidencePMID:9495778
present during GO:0072690Northern assay evidencePMID:9974219
present during GO:0072690Northern assay evidencePMID:12855726
increased during GO:0071472Northern assay evidencePMID:9495778
increased during GO:0034599Northern assay evidencePMID:9974219

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
5621.36during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
12509.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
65762during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
67707during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
74016during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
66957during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
65384during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
4.4during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:1215358243
heteromeric(2) PMID:1215358239
heteromeric(3) PMID:121535824
Misc
DescriptionQualifierReferenceCount
promoter contains CRE element PMID:151643622
target of SAPK pathway PMID:151643624
promoter contains HSE element PMID:151643621
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
vps35retromer complex subunit Vps35 Positive GeneticPMID:22681890
atf21transcription factor, Atf-CREB family Atf21 Positive GeneticPMID:22681890
SPAC56F8.02AMP binding enzyme (predicted) Negative GeneticPMID:22681890
SPAC4A8.10lipase (predicted) Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
SPAC212.02sequence orphan Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
rhn1RNA polymerase II transcription termination factor homolog Positive GeneticPMID:22681890
SPBC11B10.08WW domain containing conserved fungal protein Negative GeneticPMID:22681890
prp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
SPBC25B2.08sequence orphan Negative GeneticPMID:22681890
alp16gamma tubulin complex subunit Alp16 Negative GeneticPMID:22681890
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
dad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
sec74guanyl-nucleotide exchange factor Sec74 (predicted) Negative GeneticPMID:22681890
pop2F-box/WD repeat protein Pop2 Positive GeneticPMID:22681890
sgf29SAGA complex subunit Sgf29 Negative GeneticPMID:22681890
rpt419S proteasome regulatory subunit Rpt4 (predicted) Positive GeneticPMID:22681890
sts5RNB-like protein Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
vrp1verprolin Positive GeneticPMID:22681890
bob1prefoldin subunit 5 (predicted) Positive GeneticPMID:22681890
spp27RNA polymerase I upstream activation factor complex subunit Spp27 Positive GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
csn71COP9/signalosome complex subunit 7a (predicted) Positive GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
SPBPB2B2.17cS. pombe specific 5Tm protein family Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tps1alpha,alpha-trehalose-phosphate synthase Co-fractionationPMID:12153582
Affinity Capture-Western
Affinity Capture-WesternPMID:15965643
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
tpp1trehalose-6-phosphate phosphatase Tpp1 Co-fractionationPMID:12153582
Affinity Capture-Western
ntp1alpha,alpha-trehalase Ntp1 Affinity Capture-WesternPMID:12153582
External References
Database Identifier Description
NBRP SPBC660.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC660.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC660.07 BioGRID Interaction Datasets
Expression Viewer SPBC660.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC660.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC660.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC660.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC660.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC660.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC660.07 Cell Cycle Data
GEO SPBC660.07 GEO profiles
PInt SPBC660.07 Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz3.2.1.28Integrated relational Enzyme database
Rhea3.2.1.28Annotated reactions database
EntrezGene2541115alpha,alpha-trehalase Ntp1
WikiGene2541115alpha,alpha-trehalase Ntp1
SPD / RIKEN50/50A10Orfeome Localization Data
UniProtKB/SwissProtO42893Neutral trehalase
ModBaseO42893Database of comparative protein structure models
STRINGO42893Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595086alpha,alpha-trehalase Ntp1
RefSeq mRNANM_001020993972h- alpha,alpha-trehalase Ntp1 (ntp1), mRNA
European Nucleotide ArchiveD89273ENA EMBL mapping
European Nucleotide ArchiveBAA13934ENA Protein Mapping
European Nucleotide ArchiveBAA87141ENA Protein Mapping
European Nucleotide ArchiveBAA87141.1ENA Protein Mapping
European Nucleotide ArchiveCAA11904ENA Protein Mapping
European Nucleotide ArchiveCAA11904.1ENA Protein Mapping
European Nucleotide ArchiveCAA22527ENA Protein Mapping
European Nucleotide ArchiveCAA22527.1ENA Protein Mapping
UniParcUPI000013734DUniProt Archive

Literature for ntp1

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014