ntp1 (SPBC660.07)


Gene Standard Namentp1 Characterisation Statuspublished
Systematic IDSPBC660.07 Feature Typeprotein coding
Synonyms Name Description
Productalpha,alpha-trehalase Ntp1 Product Size735aa, 84.60 kDa
Genomic Location Chromosome II, 206580-209641 (3062nt); CDS:207078-209285 (2208nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
normal during cellular response to freezingntp1ΔNull2
normal growth on ethanolntp1ΔNull2
normal mating efficiencyntp1ΔNull24
normal vegetative cell population growth ratentp1ΔNull63
resistance to heat shock during vegetative growthntp1ΔNull7
resistance to salt stressntp1ΔNull7
resistance to water deprivationntp1ΔNull2
sensitive to phlorizin during spore germinationntp1ΔNull1
viable vegetative cell populationntp1ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
abolished alpha,alpha-trehalase activityS51A (S51A)Not specified1
S6A (S6A)Not specified
S71A (S71A)Not specified
alpha,alpha-trehalase activity increase abolished during cellular response to heatntp1ΔNull2
S51A (S51A)Not specified
S6A (S6A)Not specified
S71A (S71A)Not specified
S71D (S71D)Not specified
alpha,alpha-trehalase activity increase abolished during cellular response to nutrientntp1ΔNull3
alpha,alpha-trehalase activity increase abolished during cellular response to salt stressS51A (S51A)Not specified2
S6A (S6A)Not specified
S71A (S71A)Not specified
S71D (S71D)Not specified
decreased alpha,alpha-trehalase activity during cellular response to heatS41A (S41A)Not specified1
decreased alpha,alpha-trehalase activity during cellular response to salt stressS41A (S41A)Not specified1
decreased RNA level during cellular response to hydrogen peroxide20
affecting ntp1CRE-like promoter mutant (TGACGTAG mutated to TGACcTAG)Not specified
decreased RNA level during cellular response to salt stress12
affecting ntp1CRE-like promoter mutant (TGACGTAG mutated to TGACcTAG)Not specified
decreased spore germinationntp1ΔNull28
increased cellular trehalose level during cellular response to osmotic stressntp1ΔNull1
S41A (S41A)Not specified
S51A (S51A)Not specified
S6A (S6A)Not specified
S71A (S71A)Not specified
S71D (S71D)Not specified
increased cellular trehalose level during heat shockntp1ΔNull3
S71A (S71A)Not specified
S71D (S71D)Not specified
increased cellular trehalose level during vegetative growthntp1ΔNull3
S51A (S51A)Not specified
S6A (S6A)Not specified
S71A (S71A)Not specified
S71D (S71D)Not specified
increased cellular trehalose level in sporentp1ΔNull1
normal alpha,alpha-trehalase activityI104A (I104A)Not specified6
S41A (S41A)Not specified
normal alpha,alpha-trehalase activity increase during cellular response to heat stressI104A (I104A)Not specified3
normal alpha,alpha-trehalase activity increase during cellular response to salt stressI104A (I104A)Not specified4
normal cellular response to heat shockntp1ΔNull3
normal cellular trehalose levelI104A (I104A)Not specified4
S41A (S41A)Not specified
normal cellular trehalose level during cellular response to heatI104A (I104A)Not specified1
S6A (S6A)Not specified
normal cellular trehalose level during cellular response to salt stressI104A (I104A)Not specified3
normal protein binding56
affecting tps1 and ntp1S41A (S41A)Not specified
affecting tps1 and ntp1S51A (S51A)Not specified
affecting tps1 and ntp1S6A (S6A)Not specified
affecting tps1 and ntp1S71A (S71A)Not specified
affecting tps1 and ntp1S71D (S71D)Not specified
normal spore germinationntp1ΔNull17
normal vegetative cell morphologyntp1ΔNull3585
viable vegetative cell with normal cell morphologyntp1ΔNull3093
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1 PMID:12855726
FYPO affected by mutation in mcs4 response regulator Mcs4 PMID:9974219
FYPO affected by mutation in ntp1 alpha,alpha-trehalase Ntp1 PMID:12855726
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3 PMID:12855726
FYPO affected by mutation in pcr1 transcription factor Pcr1 PMID:12855726
FYPO affected by mutation in sty1 MAP kinase Sty1 PMID:12855726
FYPO affected by mutation in sty1 MAP kinase Sty1 PMID:9974219
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:9974219
FYPO affected by mutation in wis4 MAP kinase kinase kinase Wis4 PMID:9974219
GO regulated by atf1 transcription factor, Atf-CREB family Atf1 PMID:15164362
GO regulated by pcr1 transcription factor Pcr1 PMID:15164362
GO regulated by sty1 MAP kinase Sty1 PMID:15164362
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons206580..209641
mRNA206580..209641
5' UTR206580..207077PMID:21511999
CDS207078..209285
3' UTR209286..209641PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01204 Pfam IPR001661 Glycoside hydrolase, family 37 146 699 1
PF07492 Pfam IPR011120 Neutral trehalase Ca2+ binding 89 118 1
PS00928 Prosite Patterns IPR018232 Glycoside hydrolase, family 37, conserved site 601 610 1
PS00927 Prosite Patterns IPR018232 Glycoside hydrolase, family 37, conserved site 280 293 1
PTHR23403 HMMPANTHER IPR001661 Glycoside hydrolase, family 37 41 731 1
PTHR23403:SF6 HMMPANTHER 41 731 1
SSF48208 SuperFamily IPR008928 Six-hairpin glycosidase-like 173 695 9
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 521 537 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 390 407 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 445 462 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 682 695 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 469 486 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 594 610 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 322 340 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 115.11 Da
Charge -5.00
Isoelectric point 6.03
Molecular weight 84.60 kDa
Number of residues 735
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS731
present during mitotic M phaseS16
S16, S731
Annotation ExtensionEvidenceResidueReference
experimental evidence S16 PMID:24763107
present during mitotic M phase experimental evidence S16 PMID:21712547
experimental evidence S731 PMID:24763107
present during mitotic M phase experimental evidence S731 PMID:21712547
O-phospho-L-threonineT25 698
Annotation ExtensionEvidenceResidueReference
experimental evidence T25 PMID:24763107
phosphorylated residue 1926
added during cellular response to oxidative stress, required for alpha,alpha-trehalase activity
Annotation ExtensionEvidenceResidueReference
NAS PMID:15965654
IDA PMID:19547744
added during cellular response to oxidative stress
required for alpha,alpha-trehalase activity
IDA PMID:9974219
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0070301Northern assay evidencePMID:12855726
increased during GO:0034599expression microarray evidencePMID:12529438
increased during GO:0034605Northern assay evidencePMID:9729425
increased during GO:0071470expression microarray evidencePMID:12529438
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
present during GO:0072690Northern assay evidencePMID:12855726
increased during GO:0071472Northern assay evidencePMID:12855726
increased during GO:0071472Northern assay evidencePMID:9729425
increased during GO:0034605expression microarray evidencePMID:12529438
increased during GO:0071472Northern assay evidencePMID:9495778
increased during GO:0034605Northern assay evidencePMID:9495778
present during GO:0072690Northern assay evidencePMID:9495778
increased during GO:0034599Northern assay evidencePMID:9974219
present during GO:0072690Northern assay evidencePMID:9974219

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
66957during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
65762during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
65384during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
74016during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5621.36during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
67707during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
12509.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.4during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:1215358249
heteromeric(2) PMID:1215358241
heteromeric(3) PMID:121535824
Misc
DescriptionQualifierReferenceCount
promoter contains CRE element PMID:151643622
target of SAPK pathway PMID:151643624
promoter contains HSE element PMID:151643621
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3430
conserved in vertebrates3405
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesntp1alpha,alpha-trehalase Ntp1 Affinity Capture-WesternPMID:12153582
affinity capturestpp1trehalose-6-phosphate phosphatase Tpp1 Affinity Capture-WesternPMID:12153582
co-fractionates withtpp1trehalose-6-phosphate phosphatase Tpp1 Co-fractionationPMID:12153582
affinity captured bytps1alpha,alpha-trehalose-phosphate synthase Affinity Capture-WesternPMID:12153582
co-fractionates withtps1alpha,alpha-trehalose-phosphate synthase Co-fractionationPMID:12153582
affinity capturestps1alpha,alpha-trehalose-phosphate synthase Affinity Capture-WesternPMID:15965643
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withalp16gamma tubulin complex subunit Alp16 Negative GeneticPMID:22681890
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
positive genetic interaction withatf21transcription factor, Atf-CREB family Atf21 Positive GeneticPMID:22681890
positive genetic interaction withbob1prefoldin subunit 5 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcsn71COP9/signalosome complex subunit 7a (predicted) Positive GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
positive genetic interaction withhus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
negative genetic interaction withngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
positive genetic interaction withpop2F-box/WD repeat protein Pop2 Positive GeneticPMID:22681890
positive genetic interaction withprp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
positive genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
positive genetic interaction withrhn1RNA polymerase II transcription termination factor homolog Positive GeneticPMID:22681890
negative genetic interaction withrog1acylglycerol lipase (predicted) Negative GeneticPMID:22681890
positive genetic interaction withrpt419S proteasome regulatory subunit Rpt4 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withsec74guanyl-nucleotide exchange factor Sec74 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsgf29SAGA complex subunit Sgf29 Negative GeneticPMID:22681890
negative genetic interaction withSPAC212.02Schizosaccharomyces pombe specific protein Negative GeneticPMID:22681890
negative genetic interaction withSPAC56F8.02AMP binding enzyme (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC11B10.08WW domain containing conserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withSPBC25B2.08Schizosaccharomyces pombe specific protein Negative GeneticPMID:22681890
negative genetic interaction withSPBPB2B2.17cS. pombe specific 5Tm protein family Negative GeneticPMID:22681890
negative genetic interaction withspc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
positive genetic interaction withspp27RNA polymerase I upstream activation factor complex subunit Spp27 Positive GeneticPMID:22681890
negative genetic interaction withsts5RNB-like protein Negative GeneticPMID:22681890
positive genetic interaction withvps35retromer complex subunit Vps35 Positive GeneticPMID:22681890
positive genetic interaction withvrp1verprolin Positive GeneticPMID:22681890
positive genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC660.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC660.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC660.07 BioGRID Interaction Datasets
Expression Viewer SPBC660.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC660.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC660.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC660.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC660.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC660.07 Transcriptome Viewer (Bähler Lab)
GEO SPBC660.07 GEO profiles
PInt SPBC660.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC660.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC660.07 Fission yeast phenotypic data & analysis
Cyclebase SPBC660.07.1 Cell Cycle Data
IntEnz3.2.1.28Integrated relational Enzyme database
Rhea3.2.1.28Annotated reactions database
SPD / RIKEN50/50A10Orfeome Localization Data
UniProtKB/SwissProtO42893Neutral trehalase
ModBaseO42893Database of comparative protein structure models
STRINGO42893Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595086alpha,alpha-trehalase Ntp1
RefSeq mRNANM_001020993972h- alpha,alpha-trehalase Ntp1 (ntp1), mRNA
European Nucleotide ArchiveD89273ENA EMBL mapping
European Nucleotide ArchiveBAA87141.1ENA Protein Mapping
European Nucleotide ArchiveCAA11904.1ENA Protein Mapping
European Nucleotide ArchiveCAA22527.1ENA Protein Mapping
UniParcUPI000013734DUniProt Archive

Literature for ntp1

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015