ntp1 (SPBC660.07)


Gene Standard Namentp1 Characterisation Statuspublished
Systematic IDSPBC660.07 Feature Typeprotein coding
Synonyms Name Description
Productalpha,alpha-trehalase Ntp1 Product Size735aa, 84.60 kDa
Genomic Location Chromosome II, 206580-209641 (3062nt); CDS:207078-209285 (2208nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004555alpha,alpha-trehalase activityIDAPMID:121535821
during cellular response to glucose stimulusIMPPMID:9495778
during cellular response to heatIMPPMID:9495778
IMPPMID:10223994
GO:0005509calcium ion binding52
residue 99|108IDAPMID:12943532
GO:0005515protein bindingIPItpp1PMID:12383265887
IPIntp1PMID:12153582
IPItps1PMID:12383265
IPItpp1PMID:12153582
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030437ascospore formationIMPPMID:1109428999
GO:0005993trehalose catabolic process1
during cellular response to oxidative stressIMPPMID:9974219
GO:0090442trehalose catabolism in response to heat stressIMPPMID:102239941
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722317
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001454normal cell population growth during cellular response to freezingCell growth assayntp1ΔNullPECO:0000125, PECO:0000126, PECO:0000102PMID:102239942
FYPO:0000960normal cell population growth on ethanolCell growth assayntp1ΔNullPECO:0000126, PECO:0000102PMID:102239942
FYPO:0001420normal vegetative cell population growth rateCell growth assayntp1ΔNullPECO:0000137PMID:949577829
Cell growth assayntp1ΔNullPECO:0000126PMID:9495778
FYPO:0001090resistance to heat shock during vegetative growthCell growth assayntp1ΔNullPECO:0000168PMID:94957784
Cell growth assayntp1ΔNullPECO:0000103, PECO:0000126, PECO:0000117PMID:10223994
FYPO:0000852resistance to salt stressCell growth assayntp1ΔNullPECO:0000103, PECO:0000137, PECO:0000166PMID:94957785
Cell growth assayntp1ΔNullPECO:0000102, PECO:0000166, PECO:0000137PMID:9495778
FYPO:0001452resistance to water deprivationCell growth assayntp1ΔNullPECO:0000125, PECO:0000126, PECO:0000102PMID:102239942
FYPO:0002684sensitive to phlorizin during spore germinationCell growth assayntp1ΔNullPMID:110942891
FYPO:0002060viable vegetative cell populationMicroscopyntp1ΔNullPMID:204732893755
Microscopyntp1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001482alpha,alpha-trehalase activity increase abolished during cellular response to heatEnzyme assay datantp1ΔNullPMID:94957782
FYPO:0001481alpha,alpha-trehalase activity increase abolished during cellular response to nutrientEnzyme assay datantp1ΔNullPMID:94957782
FYPO:0000581decreased spore germinationCell growth assayntp1ΔNullPECO:0000103PMID:1109428912
FYPO:0001502increased cellular trehalose level during cellular response to osmotic stressEnzyme assay datantp1ΔNullPECO:0000137, PECO:0000166PMID:94957781
FYPO:0001445increased cellular trehalose level during heat shockEnzyme assay datantp1ΔNullPECO:0000125, PECO:0000126, PECO:0000117PMID:102239943
Enzyme assay datantp1ΔNullPMID:9495778
FYPO:0001444increased cellular trehalose level during vegetative growthEnzyme assay datantp1ΔNullPECO:0000126, PECO:0000125PMID:102239943
Enzyme assay datantp1ΔNullPECO:0000137PMID:9495778
FYPO:0002686increased cellular trehalose level in sporeCell growth assayntp1ΔNullPMID:110942891
FYPO:0001091normal cellular response to heat shockCell growth assayntp1ΔNullPECO:0000004PMID:94957782
FYPO:0001147normal mating efficiencyCell growth assayntp1ΔNullPMID:949577812
FYPO:0000579normal spore germinationCell growth assayntp1ΔNullPMID:94957787
FYPO:0001315normal vegetative cell morphologyMicroscopyntp1ΔNullPMID:949577842
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopyntp1ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Target Of
RelationshipGeneProductReference
regulated by atf1 transcription factor, Atf-CREB family Atf1 PMID:15164362
regulated by pcr1 transcription factor Pcr1 PMID:15164362
regulated by sty1 MAP kinase Sty1 PMID:15164362
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1206580209641

UTRs

Region Start End Reference
three_prime_UTR209286209641PMID:21511999
five_prime_UTR206580207077PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07492 Pfam IPR011120 Neutral trehalase Ca2+ binding 89 118 1
PF01204 Pfam IPR001661 Glycoside hydrolase, family 37 146 699 1
PS00927 Prosite Patterns IPR018232 Glycoside hydrolase, family 37 280 293 1
PS00928 Prosite Patterns IPR018232 Glycoside hydrolase, family 37 601 610 1
PTHR23403 HMMPANTHER IPR001661 Glycoside hydrolase, family 37 51 724 1
PTHR23403:SF1 HMMPANTHER 51 724 1
SSF48208 SuperFamily IPR008928 173 695 9
Low complexity (SEG) seg 512 520
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 445 462 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 390 407 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 682 695 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 322 340 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 469 486 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 521 537 1
PR00744 PRINTS IPR001661 Glycoside hydrolase, family 37 594 610 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 115.11 Da
Charge -5.00
Isoelectric point 6.03
Molecular weight 84.60 kDa
Number of residues 735
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueIDAPMID:99742191192
required_for alpha,alpha-trehalase activity, added_during cellular response to oxidative stress
added_during cellular response to oxidative stress, required_for alpha,alpha-trehalase activityIDAPMID:9974219
PMID:19547744
PMID:15965654
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreasedexpression microarray evidencePMID:12529438
during GO:0071470
increasedNorthern assay evidencePMID:9495778
during GO:0071472
increasedNorthern assay evidencePMID:9974219
during GO:0034599
presentNorthern assay evidencePMID:9495778
during GO:0072690
increasedNorthern assay evidencePMID:9495778
during GO:0034605
increasedNorthern assay evidencePMID:9729425
during GO:0071472
increasedexpression microarray evidencePMID:12529438
during GO:0034599
increased,
in presence of methyl methanesulfonate
expression microarray evidencePMID:12529438
increasedexpression microarray evidencePMID:12529438
during GO:0034605
increased,
in presence of cadmium sulfate
expression microarray evidencePMID:12529438
presentNorthern assay evidencePMID:9974219
during GO:0072690
increasedNorthern assay evidencePMID:9729425
during GO:0034605

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
12509.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
5621.36during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
4.4during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
homodimeric PMID:1215358217
heterodimeric PMID:121535825
trimeric PMID:121535823
Misc
DescriptionQualifierReferenceCount
promoter contains CRE element PMID:151643622
target of SAPK pathway PMID:151643624
promoter contains HSE element PMID:151643621
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3394
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
vps35retromer complex subunit Vps35 Positive GeneticPMID:22681890
atf21transcription factor, Atf-CREB family Atf21 Positive GeneticPMID:22681890
SPAC56F8.02AMP binding enzyme (predicted) Negative GeneticPMID:22681890
SPAC4A8.10lipase (predicted) Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
SPAC212.02sequence orphan Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
rhn1RNA polymerase II transcription termination factor homolog Positive GeneticPMID:22681890
SPBC11B10.08WW domain containing conserved fungal protein Negative GeneticPMID:22681890
prp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
SPBC25B2.08sequence orphan Negative GeneticPMID:22681890
alp16gamma tubulin complex subunit Alp16 Negative GeneticPMID:22681890
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
dad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
sec74guanyl-nucleotide exchange factor Sec74 (predicted) Negative GeneticPMID:22681890
pop2F-box/WD repeat protein Pop2 Positive GeneticPMID:22681890
sgf29SAGA complex subunit Sgf29 Negative GeneticPMID:22681890
rpt419S proteasome regulatory subunit Rpt4 (predicted) Positive GeneticPMID:22681890
sts5RNB-like protein Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
vrp1verprolin Positive GeneticPMID:22681890
bob1prefoldin subunit 5 (predicted) Positive GeneticPMID:22681890
spp27RNA polymerase I upstream activation factor complex subunit Spp27 Positive GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
csn71COP9/signalosome complex subunit 7a (predicted) Positive GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
SPBPB2B2.17cS. pombe specific 5Tm protein family Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tps1alpha,alpha-trehalose-phosphate synthase Affinity Capture-WesternPMID:12153582
Co-fractionation
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
tpp1trehalose-6-phosphate phosphatase Tpp1 Co-fractionationPMID:12153582
Affinity Capture-Western
External References
Database Identifier Description
NBRP SPBC660.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC660.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC660.07 BioGRID Interaction Datasets
Expression Viewer SPBC660.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC660.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC660.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC660.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC660.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC660.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC660.07 Cell Cycle Data
GEO SPBC660.07 GEO profiles
PInt SPBC660.07 Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz3.2.1.28Integrated relational Enzyme database
Rhea3.2.1.28Annotated reactions database
EntrezGene2541115alpha,alpha-trehalase Ntp1
WikiGene2541115alpha,alpha-trehalase Ntp1
SPD / RIKEN50/50A10Orfeome Localization Data
UniProtKB/SwissProtO42893Neutral trehalase
ModBaseO42893Database of comparative protein structure models
StringO42893Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595086alpha,alpha-trehalase Ntp1
RefSeq mRNANM_001020993972h- alpha,alpha-trehalase Ntp1 (ntp1), mRNA
European Nucleotide ArchiveBAA13934ENA Protein Mapping
European Nucleotide ArchiveBAA87141ENA Protein Mapping
European Nucleotide ArchiveCAA11904ENA Protein Mapping
European Nucleotide ArchiveCAA22527ENA Protein Mapping
UniParcUPI000013734DUniProt Archive

Literature for ntp1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014