mik1 (SPBC660.14)

Gene Standard Namemik1 Characterisation Statuspublished
Systematic IDSPBC660.14 Feature Typeprotein coding
Synonyms Name DescriptionMitosis Inhibitory Kinase
Productmitotic inhibitor kinase Mik1 Product Size581aa, 65.93 kDa
Genomic Location Chromosome II, 221358-224117 (2760nt); CDS:221699-223512 (1814nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine kinase activity112
Annotation ExtensionEvidenceWith/FromReference
protein tyrosine kinase activity9
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
negative regulation of G2/M transition of mitotic cell cycle39
Annotation ExtensionEvidenceWith/FromReference
peptidyl-tyrosine phosphorylation11
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationnot recorded (wild type)1427
normal growth on methyl methanesulfonatemik1ΔNull25
normal vegetative cell population growthmik1ΔNull685
sensitive to hydroxyureamik1ΔNull518
expressivity FYPO_EXT:0000002mik1ΔNull
expressivity FYPO_EXT:0000003mik1ΔNull
sensitive to miconazolenot recorded (wild type)37
viable vegetative cell populationmik1ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
abolished protein kinase activityK320R (K320R)Not specified17
cut during cellular response to hydroxyurea43
penetrance FYPO_EXT:0000002mik1ΔNull
normal cell cycle regulation during cellular response to hydroxyureamik1ΔNull12
normal cell cycle regulation during cellular response to ionizing radiationmik1ΔNull15
normal S-phase DNA damage checkpointmik1ΔNull17
normal vegetative cell morphologymik1ΔNull3580
viable elongated vegetative cellmik1+ (wild type)Overexpression231
viable vegetative cell with normal cell morphology3092
penetrance FYPO_EXT:0000001mik1ΔNull
Target Of
GO regulated by cdc10 MBF transcription factor complex subunit Cdc10 PMID:19750219
GO regulated by rep1 MBF transcription factor activator Rep1 PMID:19750219
GO regulated by rep2 MBF transcription factor activator Rep2 PMID:19750219
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 291 556 109
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 289 558 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 413 425 97
PS50011 Prosite Profiles IPR000719 Protein kinase domain 289 561 110
PTHR11042 HMMPANTHER 14 581 5
PTHR11042:SF73 HMMPANTHER 14 581 2
1.10.510.10 Gene3D 402 558 112 Gene3D 284 401 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 286 557 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.48 Da
Charge -2.00
Isoelectric point 6.33
Molecular weight 65.93 kDa
Number of residues 581

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS137
S174, S177
present during mitotic M phaseS130
present during mitotic M phaseS132
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S130 PMID:21712547
present during mitotic M phase experimental evidence S132 PMID:21712547
present during mitotic M phase experimental evidence S137 PMID:21712547
experimental evidence S174 PMID:24763107
experimental evidence S177 PMID:24763107
O-phospho-L-threonine 693
present during mitotic M phaseT133
present during mitotic M phaseT129
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T129 PMID:21712547
present during mitotic M phase experimental evidence T133 PMID:21712547
Gene Expression

Qualitative Gene Expression

RNA levelincreased during GO:0000084experimental evidencePMID:PMPB:0

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
14964during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.72during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
0.056during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hsp90Hsp90 chaperone Affinity Capture-WesternPMID:11298745
wos2p23 homolog, predicted co-chaperone Wos2 Affinity Capture-WesternPMID:11298745
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:7982971
ubi4ubiquitin Affinity Capture-WesternPMID:10637286
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
mrf1mitochondrial peptide chain release factor (predicted) Negative GeneticPMID:22681890
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
abc2glutathione S-conjugate-exporting ATPase Abc2 Positive GeneticPMID:22681890
scs7sphingosine hydroxylase Scs7 Negative GeneticPMID:22681890
SPAC29A4.02ctranslation elongation factor EF-1 gamma subunit Phenotypic SuppressionPMID:14985109
cig2G1/S-specific B-type cyclin Cig2 Synthetic RescuePMID:8455610
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
ucp12ATP-dependent RNA helicase Ucp12 (predicted) Positive GeneticPMID:22681890
SPAC1805.14Schizosaccharomyces specific protein Negative GeneticPMID:22681890
sck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
cdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Dosage LethalityPMID:8515818
atp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
mpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
cwf16splicing factor Cwf16 Negative GeneticPMID:22681890
rps170140S ribosomal protein S17 (predicted) Negative GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
tor1phosphatidylinositol kinase Tor1 Negative GeneticPMID:22681890
SPAC212.02Schizosaccharomyces pombe specific protein Negative GeneticPMID:22681890
wee1M phase inhibitor protein kinase Wee1 Synthetic LethalityPMID:11809834
Synthetic Growth DefectPMID:11029045
Phenotypic EnhancementPMID:10947840
Synthetic LethalityPMID:1706223
Dosage Rescue
Synthetic LethalityPMID:9398669
yap18ENTH/VHS domain protein (predicted) Positive GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
pam17TIM23 translocase complex-associated motor subunit Pam17 (predicted) Positive GeneticPMID:22681890
vps13achorein homolog (predicted) Positive GeneticPMID:22681890
pfl4cell surface glycoprotein, adhesion molecule (predicted) Positive GeneticPMID:22681890
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:10637286
Synthetic RescuePMID:24861625
atg6beclin family protein, involved in autophagy Negative GeneticPMID:22681890
far10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
hrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
rox3mediator complex subunit Med19/Rox3 Negative GeneticPMID:22681890
cdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic SuppressionPMID:10637286
Synthetic Growth DefectPMID:22806395
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:22681890
SPCC1840.09NAD dependent epimerase/dehydratase family protein Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
Negative GeneticPMID:22681890
srw1fizzy-related CDK inhibitor Srw1 Dosage RescuePMID:9398669
SPCC584.01csulfite reductase NADPH flavoprotein subunit (predicted) Positive GeneticPMID:22681890
cph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
ppr2mitochondrial PPR repeat protein Ppr2 Negative GeneticPMID:22681890
erg28Erg28 protein (predicted) Negative GeneticPMID:22681890
csn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
res2MBF transcription factor complex subunit Res2 Synthetic RescuePMID:11029045
SPBC365.16conserved protein Negative GeneticPMID:22681890
hmt2sulfide-quinone oxidoreductase Negative GeneticPMID:22681890
rpl210260S ribosomal protein L21 (predicted) Negative GeneticPMID:22681890
cdc10MBF transcription factor complex subunit Cdc10 Phenotypic SuppressionPMID:11513869
SPAC17H9.08mitochondrial coenzyme A transmembrane transporter (predicted) Negative GeneticPMID:22681890
pof3F-box protein Pof3 Phenotypic EnhancementPMID:11809834
tpp1trehalose-6-phosphate phosphatase Tpp1 Negative GeneticPMID:22681890
ran1serine/threonine protein kinase Ran1 Phenotypic SuppressionPMID:10521402
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC660.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC660.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC660.14 BioGRID Interaction Datasets
Expression Viewer SPBC660.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC660.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC660.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC660.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC660.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC660.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC660.14 Cell Cycle Data
GEO SPBC660.14 GEO profiles
PInt SPBC660.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC660.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC660.14 Fission yeast phenotypic data & analysis
SPD / RIKEN36/36F03Orfeome Localization Data
UniProtKB/SwissProtP30290Mitosis inhibitor protein kinase mik1
ModBaseP30290Database of comparative protein structure models
STRINGP30290Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595093mitotic inhibitor kinase Mik1
RefSeq mRNANM_001021000972h- mitotic inhibitor kinase Mik1 (mik1), mRNA
European Nucleotide ArchiveAAA91278.1ENA Protein Mapping
European Nucleotide ArchiveCAA22534.1ENA Protein Mapping
UniParcUPI000012F108UniProt Archive

Literature for mik1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015