mik1 (SPBC660.14)


Gene Standard Namemik1 Characterisation Statuspublished
Systematic IDSPBC660.14 Feature Typeprotein coding
Synonyms Name DescriptionMitosis Inhibitory Kinase
Productmitotic inhibitor kinase Mik1 Product Size581aa, 65.93 kDa
Genomic Location Chromosome II, 221358-224117 (2760nt); CDS:221699-223512 (1814nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
metal ion binding745
Annotation ExtensionEvidenceWith/FromReference
protein tyrosine kinase activity9
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
negative regulation of G2/M transition of mitotic cell cycle41
Annotation ExtensionEvidenceWith/FromReference
peptidyl-tyrosine phosphorylation11
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2695
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationmik1+ (wild type)Overexpression1435
normal growth on methyl methanesulfonatemik1ΔNull28
normal vegetative cell population growthmik1ΔNull724
sensitive to hydroxyureamik1ΔNull534
sensitive to miconazolemik1+ (wild type)Overexpression37
viable vegetative cell populationmik1ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
abolished protein kinase activity19
affecting mik1K320R (K320R)Not specified
cut during cellular response to hydroxyureamik1ΔNull44
normal cell cycle regulation during cellular response to hydroxyureamik1ΔNull12
normal cell cycle regulation during cellular response to ionizing radiationmik1ΔNull16
normal S-phase DNA damage checkpointmik1ΔNull17
viable elongated vegetative cellmik1+ (wild type)Overexpression289
viable vegetative cell with normal cell morphologymik1ΔNull3093
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in mik1 mitotic inhibitor kinase Mik1 PMID:7982971
GO regulated by cdc10 MBF transcription factor complex subunit Cdc10 PMID:19750219
GO regulated by rep1 MBF transcription factor activator Rep1 PMID:19750219
GO regulated by rep2 MBF transcription factor activator Rep2 PMID:19750219
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons221358..222907, 222976..224117
mRNA221358..224117
5' UTR221358..221698PMID:21511999
CDS221699..222907, 222976..223512
3' UTR223513..224117PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 291 556 109
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 289 558 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 413 425 97
PS50011 Prosite Profiles IPR000719 Protein kinase domain 289 561 110
PTHR11042 HMMPANTHER 14 581 5
PTHR11042:SF73 HMMPANTHER 14 581 2
3.30.200.20 Gene3D 284 401 111
1.10.510.10 Gene3D 402 558 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 286 557 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.48 Da
Charge -2.00
Isoelectric point 6.33
Molecular weight 65.93 kDa
Number of residues 581
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS137
S174, S177
present during mitotic M phaseS132
present during mitotic M phaseS130
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S130 PMID:21712547
present during mitotic M phase experimental evidence S132 PMID:21712547
present during mitotic M phase experimental evidence S137 PMID:21712547
experimental evidence S174 PMID:24763107
experimental evidence S177 PMID:24763107
O-phospho-L-threonine 698
present during mitotic M phaseT133
present during mitotic M phaseT129
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T129 PMID:21712547
present during mitotic M phase experimental evidence T133 PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0000084experimental evidencePMID:PMPB:0

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
14964during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.72during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.056during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
modifiescdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:7982971
affinity captureshsp90Hsp90 chaperone Affinity Capture-WesternPMID:11298745
affinity captured byubi4ubiquitin Affinity Capture-WesternPMID:10637286
affinity captureswos2p23 homolog, predicted co-chaperone Wos2 Affinity Capture-WesternPMID:11298745
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withabc2glutathione S-conjugate-exporting ATPase Abc2 Positive GeneticPMID:22681890
negative genetic interaction withatg6beclin family protein, involved in autophagy Negative GeneticPMID:22681890
negative genetic interaction withatp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
rescuescdc10MBF transcription factor complex subunit Cdc10 Phenotypic SuppressionPMID:11513869
rescuescdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:10637286
synthetically rescuescdc2cyclin-dependent protein kinase Cdk1/Cdc2 Synthetic RescuePMID:24861625
rescuescdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic SuppressionPMID:10637286
synthetic growth defect withcdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic Growth DefectPMID:22806395
inviable in presence of overexpressedcdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Dosage LethalityPMID:8515818
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
synthetically rescued bycig2G1/S-specific B-type cyclin Cig2 Synthetic RescuePMID:8455610
negative genetic interaction withcoq11ubiquinone biosynthesis protein Coq11 Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
negative genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
negative genetic interaction withcwf16splicing factor Cwf16 Negative GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction witherg28Erg28 protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withfar10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
negative genetic interaction withhmt2sulfide-quinone oxidoreductase Negative GeneticPMID:22681890
negative genetic interaction withhrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
synthetic growth defect withmcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
negative genetic interaction withmcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:22681890
negative genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
negative genetic interaction withmrf1mitochondrial peptide chain release factor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:22681890
positive genetic interaction withpam17TIM23 translocase complex-associated motor subunit Pam17 (predicted) Positive GeneticPMID:22681890
rescuespat1serine/threonine protein kinase Ran1/Pat1 Phenotypic SuppressionPMID:10521402
positive genetic interaction withpfl4cell surface glycoprotein, adhesion molecule (predicted) Positive GeneticPMID:22681890
enhances phenotype ofpof3F-box protein Pof3 Phenotypic EnhancementPMID:11809834
negative genetic interaction withppr2mitochondrial PPR repeat protein Ppr2 Negative GeneticPMID:22681890
negative genetic interaction withprp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
synthetically rescued byres2MBF transcription factor complex subunit Res2 Synthetic RescuePMID:11029045
negative genetic interaction withrox3mediator complex subunit Med19/Rox3 Negative GeneticPMID:22681890
negative genetic interaction withrpl210260S ribosomal protein L21 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrps170140S ribosomal protein S17 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
negative genetic interaction withscs7sphingosine hydroxylase Scs7 Negative GeneticPMID:22681890
negative genetic interaction withset1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC17H9.08mitochondrial coenzyme A transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC1805.14Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withSPAC212.02Schizosaccharomyces pombe specific protein Negative GeneticPMID:22681890
rescued bySPAC29A4.02ctranslation elongation factor EF-1 gamma subunit Phenotypic SuppressionPMID:14985109
negative genetic interaction withSPBC365.16conserved protein Negative GeneticPMID:22681890
positive genetic interaction withSPCC584.01csulfite reductase NADPH flavoprotein subunit (predicted) Positive GeneticPMID:22681890
negative genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
rescued by overexpression ofsrw1fizzy-related CDK inhibitor Srw1 Dosage RescuePMID:9398669
negative genetic interaction withtor1phosphatidylinositol kinase Tor1 Negative GeneticPMID:22681890
negative genetic interaction withtpp1trehalose-6-phosphate phosphatase Tpp1 Negative GeneticPMID:22681890
positive genetic interaction withucp12ATP-dependent RNA helicase Ucp12 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withvps1301chorein homolog Vps13a (predicted) Positive GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
synthetic lethal withwee1M phase inhibitor protein kinase Wee1 Synthetic LethalityPMID:11809834
synthetic growth defect withwee1M phase inhibitor protein kinase Wee1 Synthetic Growth DefectPMID:11029045
enhances phenotype ofwee1M phase inhibitor protein kinase Wee1 Phenotypic EnhancementPMID:10947840
overexpression rescueswee1M phase inhibitor protein kinase Wee1 Dosage RescuePMID:1706223
synthetic lethal withwee1M phase inhibitor protein kinase Wee1 Synthetic LethalityPMID:1706223
synthetic lethal withwee1M phase inhibitor protein kinase Wee1 Synthetic LethalityPMID:9398669
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
positive genetic interaction withyap18ENTH/VHS domain protein (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPBC660.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC660.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC660.14 BioGRID Interaction Datasets
Expression Viewer SPBC660.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC660.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC660.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC660.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC660.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC660.14 Transcriptome Viewer (Bähler Lab)
GEO SPBC660.14 GEO profiles
PInt SPBC660.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC660.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC660.14 Fission yeast phenotypic data & analysis
Cyclebase SPBC660.14.1 Cell Cycle Data
SPD / RIKEN36/36F03Orfeome Localization Data
UniProtKB/SwissProtP30290Mitosis inhibitor protein kinase mik1
ModBaseP30290Database of comparative protein structure models
STRINGP30290Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595093mitotic inhibitor kinase Mik1
RefSeq mRNANM_001021000972h- mitotic inhibitor kinase Mik1 (mik1), mRNA
European Nucleotide ArchiveAAA91278.1ENA Protein Mapping
European Nucleotide ArchiveCAA22534.1ENA Protein Mapping
UniParcUPI000012F108UniProt Archive

Literature for mik1

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015