mik1 (SPBC660.14)


Gene Standard Namemik1 Characterisation Statuspublished
Systematic IDSPBC660.14 Feature Typeprotein coding
Synonyms Name DescriptionMitosis Inhibitory Kinase
Productmitotic inhibitor kinase Mik1 Product Size581aa, 65.93 kDa
Genomic Location Chromosome II, 221358-224117 (2760nt); CDS:221699-223512 (1814nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037747
GO:0004674protein serine/threonine kinase activityIDAPMID:1297355112
GO:0004713protein tyrosine kinase activity9
has substrate cdc2IDAPMID:7982971
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0010972negative regulation of G2/M transition of mitotic cell cycleIGIwee1PMID:170622342
GO:0018108peptidyl-tyrosine phosphorylationIDAPMID:798297111
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722315
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000957normal growth on methyl methanesulfonateCell growth assaymik1ΔNullPECO:0000005PMID:1529745720
FYPO:0001357normal vegetative cell population growthCell growth assaymik1ΔNullPMID:16615890641
FYPO:0000088sensitive to hydroxyurea514
expressivity FYPO_EXT:0000002Cell Growth Assaymik1ΔNullPECO:0000137, PECO:0000102PMID:23697806
expressivity FYPO_EXT:0000003Cell growth assaymik1ΔNullPECO:0000137, PECO:0000005PMID:19264558
Cell growth assaymik1ΔNullPMID:1549179
FYPO:0002060viable vegetative cell populationCell growth assaymik1ΔNullPMID:17062233759
Microscopymik1ΔNullPMID:20473289
Microscopymik1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001384abolished protein kinase activityEnzyme assay dataK320R (K320R)Not specifiedPMID:798297115
FYPO:0002054cut during cellular response to hydroxyurea41
penetrance FYPO_EXT:0000002Microscopymik1ΔNullPMID:23697806
FYPO:0003530normal S-phase DNA damage checkpointFlow cytometry datamik1ΔNullPECO:0000004PMID:152974573
FYPO:0001929normal cell cycle regulation during cellular response to hydroxyureaMicroscopymik1ΔNullPMID:170622310
FYPO:0001927normal cell cycle regulation during cellular response to ionizing radiationMicroscopymik1ΔNullPMID:154917912
FYPO:0001315normal vegetative cell morphologyMicroscopymik1ΔNullPMID:17062233573
FYPO:0001492viable elongated vegetative cellMicroscopymik1+ (wild type)OverexpressionPMID:1706223221
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopymik1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
GO regulated by cdc10 MBF transcription factor complex subunit Cdc10 PMID:19750219
GO regulated by rep1 MBF transcription factor activator Rep1 PMID:19750219
GO regulated by rep2 MBF transcription factor activator Rep2 PMID:19750219
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1221358222907
2222976224117

UTRs

Region Coordinates Reference
five_prime_UTR221358..221698PMID:21511999
three_prime_UTR223513..224117PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 291 556 109
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 289 558 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 413 425 97
PS50011 Prosite Profiles IPR000719 Protein kinase domain 289 561 110
PTHR11042 HMMPANTHER 20 580 5
PTHR11042:SF21 HMMPANTHER 20 580 2
1.10.510.10 Gene3D 402 558 112
3.30.200.20 Gene3D 284 401 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 286 557 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.48 Da
Charge -2.00
Isoelectric point 6.33
Molecular weight 65.93 kDa
Number of residues 581
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS132PMID:217125471670
present during mitotic M phase
present during mitotic M phaseexperimental evidenceS130PMID:21712547
present during mitotic M phaseexperimental evidenceS137PMID:21712547
experimental evidenceS177PMID:24763107
experimental evidenceS174PMID:24763107
MOD:00047O-phospho-L-threonineexperimental evidenceT129PMID:21712547692
present during mitotic M phase
present during mitotic M phaseexperimental evidenceT133PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0000084experimental evidencePMID:PMPB:0

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
14964during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.72during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.056during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
mrf1mitochondrial peptide chain release factor (predicted) Negative GeneticPMID:22681890
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
abc2glutathione S-conjugate-exporting ATPase Abc2 Positive GeneticPMID:22681890
scs7sphingosine hydroxylase Scs7 Negative GeneticPMID:22681890
SPAC29A4.02ctranslation elongation factor EF-1 gamma subunit Phenotypic SuppressionPMID:14985109
cig2G1/S-specific B-type cyclin Cig2 Synthetic RescuePMID:8455610
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
ucp12ATP-dependent RNA helicase Ucp12 (predicted) Positive GeneticPMID:22681890
SPAC1805.14Schizosaccharomyces specific protein Negative GeneticPMID:22681890
sck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
cdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Dosage LethalityPMID:8515818
atp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
mpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
cwf16splicing factor Cwf16 Negative GeneticPMID:22681890
rps170140S ribosomal protein S17 (predicted) Negative GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
tor1phosphatidylinositol kinase Tor1 Negative GeneticPMID:22681890
SPAC212.02Schizosaccharomyces pombe specific protein Negative GeneticPMID:22681890
wee1M phase inhibitor protein kinase Wee1 Synthetic LethalityPMID:11809834
Synthetic Growth DefectPMID:11029045
Phenotypic EnhancementPMID:10947840
Synthetic LethalityPMID:1706223
Dosage Rescue
Synthetic LethalityPMID:9398669
yap18ENTH/VHS domain protein (predicted) Positive GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
pam17TIM23 translocase complex-associated motor subunit Pam17 (predicted) Positive GeneticPMID:22681890
vps13achorein homolog (predicted) Positive GeneticPMID:22681890
pfl4cell surface glycoprotein, adhesion molecule (predicted) Positive GeneticPMID:22681890
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:10637286
Synthetic RescuePMID:24861625
atg6beclin family protein, involved in autophagy Negative GeneticPMID:22681890
far10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
hrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
rox3mediator complex subunit Med19/Rox3 Negative GeneticPMID:22681890
cdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic SuppressionPMID:10637286
Synthetic Growth DefectPMID:22806395
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:22681890
SPCC1840.09NAD dependent epimerase/dehydratase family protein Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
Negative GeneticPMID:22681890
srw1CDK inhibitor Srw1 Dosage RescuePMID:9398669
SPCC584.01csulfite reductase NADPH flavoprotein subunit (predicted) Positive GeneticPMID:22681890
cph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
ppr2mitochondrial PPR repeat protein Ppr2 Negative GeneticPMID:22681890
erg28Erg28 protein (predicted) Negative GeneticPMID:22681890
csn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
res2MBF transcription factor complex subunit Res2 Synthetic RescuePMID:11029045
SPBC365.16conserved protein Negative GeneticPMID:22681890
hmt2sulfide-quinone oxidoreductase Negative GeneticPMID:22681890
rpl210260S ribosomal protein L21 (predicted) Negative GeneticPMID:22681890
cdc10MBF transcription factor complex subunit Cdc10 Phenotypic SuppressionPMID:11513869
SPAC17H9.08mitochondrial coenzyme A transporter (predicted) Negative GeneticPMID:22681890
pof3F-box protein Pof3 Phenotypic EnhancementPMID:11809834
tpp1trehalose-6-phosphate phosphatase Tpp1 Negative GeneticPMID:22681890
ran1serine/threonine protein kinase Ran1 Phenotypic SuppressionPMID:10521402
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hsp90Hsp90 chaperone Affinity Capture-WesternPMID:11298745
wos2p23 homolog, predicted co-chaperone Wos2 Affinity Capture-WesternPMID:11298745
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:7982971
ubi4ubiquitin Affinity Capture-WesternPMID:10637286
External References
Database Identifier Description
NBRP SPBC660.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC660.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC660.14 BioGRID Interaction Datasets
Expression Viewer SPBC660.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC660.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC660.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC660.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC660.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC660.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC660.14 Cell Cycle Data
GEO SPBC660.14 GEO profiles
PInt SPBC660.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC660.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC660.14 Fission yeast phenotypic data & analysis
SPD / RIKEN36/36F03Orfeome Localization Data
UniProtKB/SwissProtP30290Mitosis inhibitor protein kinase mik1
ModBaseP30290Database of comparative protein structure models
STRINGP30290Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595093mitotic inhibitor kinase Mik1
RefSeq mRNANM_001021000972h- mitotic inhibitor kinase Mik1 (mik1), mRNA
European Nucleotide ArchiveAAA91278.1ENA Protein Mapping
European Nucleotide ArchiveCAA22534.1ENA Protein Mapping
UniParcUPI000012F108UniProt Archive

Literature for mik1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014