psf2 (SPBC725.13c)


Gene Standard Namepsf2 Characterisation Statuspublished
Systematic IDSPBC725.13c Feature Typeprotein coding
Synonymsbsh3, dre13 Name DescriptionDefect in REplication
ProductGINS complex subunit Psf2 Product Size183aa, 21.34 kDa
Genomic Location Chromosome II, 1229239-1228148 (1092nt); CDS:1229027-1228476 (552nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006270DNA replication initiationTASPMID:1910942931
GO:0034613cellular protein localization556
localizes bir1IMPGO_REF:0000001
GO:0045132meiotic chromosome segregationIMPPMID:1546642158
GO:0033260nuclear cell cycle DNA replicationIMPPMID:1546642125
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000811GINS complexISOSGD:S000003608GO_REF:00000244
GO:0005829cytosolIDAPMID:168233722317
GO:0043596nuclear replication forkICGO:0000811GO_REF:000000163
GO:0005634nucleusIMPPMID:154664212730
IDAPMID:16199877
IDAPMID:16823372
IDAPMID:19109429
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopypsf2ΔNullPMID:204732891309
no_namePMID:16199877
Microscopypsf2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopypsf2ΔNullPECO:0000004, PECO:0000137PMID:23697806564
FYPO:0000625abnormal premeiotic DNA replicationFlow cytometry datapsf2-HBD (steroid hormone-binding domain fusion)Not specifiedPECO:0000218PMID:191094294
FYPO:0001490inviable elongated vegetative cellMicroscopypsf2ΔNullPECO:0000005, PECO:0000137PMID:23697806368
penetrance FYPO_EXT:0000003
FYPO:0001511inviable vegetative cell, abnormal cell shape, normal cell sizeMicroscopypsf2ΔNullPECO:0000137, PECO:0000005PMID:23697806198
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
FYPO:0000012mitotic G2/M transition delayFlow cytometry datapsf2-HBD (steroid hormone-binding domain fusion)Not specifiedPECO:0000218PMID:1910942913
FYPO:0000783protein mislocalized to cytoplasmMicroscopypsf2-HBD (steroid hormone-binding domain fusion)Not specifiedPECO:0000218PMID:1910942913
Microscopypsf2-HBD (steroid hormone-binding domain fusion)Not specifiedPECO:0000218PMID:19109429
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
112292391228148

UTRs

Region Start End Reference
three_prime_UTR12284751228148PMID:21511999
five_prime_UTR12292391229028PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05916 Pfam IPR021151 GINS complex, subunit Sld5 53 159 4
PTHR12772 HMMPANTHER IPR007257 GINS complex, subunit Psf2 1 181 1
SSF160059 SuperFamily 11 71 3
SSF158573 SuperFamily 72 176 4
Low complexity (SEG) seg 114 125
PIRSF028998 PIRSF IPR016906 GINS complex, subunit Psf2, subgroup 1 183 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.62 Da
Charge -13.50
Isoelectric point 4.30
Molecular weight 21.34 kDa
Number of residues 183
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
716.23during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.72during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
2.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
bir1survivin, Bir1 Dosage RescuePMID:16199877
chk1Chk1 protein kinase Synthetic Growth DefectPMID:15466421
psf3GINS complex subunit Psf3 Dosage RescuePMID:16990792
swi3replication fork protection complex subunit Swi3 Synthetic Growth DefectPMID:20176980
rad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:15466421
swi1replication fork protection complex subunit Swi1 Synthetic LethalityPMID:20176980
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:22433840
rad4BRCT domain protein Rad4 Affinity Capture-WesternPMID:22718908
mcm6MCM complex subunit Mcm6 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:22433840
cdc23MCM-associated protein Mcm10 Two-hybridPMID:22433840
psf3GINS complex subunit Psf3 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:16990792
mcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:22433840
sld5GINS complex subunit Sld5 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:16990792
Two-hybridPMID:23695164
psf1GINS complex subunit Psf1 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:16990792
sld3DNA replication pre-initiation complex subunit Sld3 Two-hybridPMID:21593208
External References
Database Identifier Description
NBRP SPBC725.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC725.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC725.13c BioGRID Interaction Datasets
Expression Viewer SPBC725.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC725.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC725.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC725.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC725.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC725.13c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC725.13c Cell Cycle Data
GEO SPBC725.13c GEO profiles
PInt SPBC725.13c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2541194GINS complex subunit Psf2
EntrezGene2541194GINS complex subunit Psf2
SPD / RIKEN05/05F08Orfeome Localization Data
UniProtKB/SwissProtO94329DNA replication complex GINS protein psf2
ModBaseO94329Database of comparative protein structure models
StringO94329Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595493GINS complex subunit Psf2
RefSeq mRNANM_001021404972h- GINS complex subunit Psf2 (psf2), mRNA
European Nucleotide ArchiveCAA22185ENA Protein Mapping
UniParcUPI000006A3B6UniProt Archive

Literature for psf2

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014