psf2 (SPBC725.13c)


Gene Standard Namepsf2 Characterisation Statuspublished
Systematic IDSPBC725.13c Feature Typeprotein coding
Synonymsbsh3, dre13 Name DescriptionDefect in REplication
ProductGINS complex subunit Psf2 Product Size183aa, 21.34 kDa
Genomic Location Chromosome II, 1229239-1228148 (1092nt); CDS:1229027-1228476 (552nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
meiotic chromosome segregation30
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication70
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication initiation33
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
GINS complex4
Annotation ExtensionEvidenceWith/FromReference
nuclear replication fork63
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationnot recorded (unrecorded)1427
psf2ΔNull

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclepsf2ΔNull794
abnormal premeiotic DNA replicationpsf2-HBD (steroid hormone-binding domain fusion)Not specified21
inviable elongated vegetative cell405
penetrance FYPO_EXT:0000003psf2ΔNull
inviable vegetative cell, abnormal cell shape, normal cell size199
penetrance FYPO_EXT:0000001, expressivity FYPO_EXT:0000003psf2ΔNull
mitotic G2/M transition delaypsf2-HBD (steroid hormone-binding domain fusion)Not specified29
protein mislocalized to cytoplasmpsf2-HBD (steroid hormone-binding domain fusion)Not specified34
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
112292391228148

UTRs

Region Coordinates Reference
five_prime_UTR1229239..1229028PMID:21511999
three_prime_UTR1228475..1228148PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05916 Pfam IPR021151 GINS complex 53 159 4
PTHR12772 HMMPANTHER IPR007257 GINS complex, subunit Psf2 1 183 1
SSF160059 SuperFamily 11 71 3
SSF158573 SuperFamily 72 176 4
PIRSF028998 PIRSF IPR016906 GINS complex, subunit Psf2, subgroup 1 183 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.62 Da
Charge -13.50
Isoelectric point 4.30
Molecular weight 21.34 kDa
Number of residues 183
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
716.23during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.72during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
sld5GINS complex subunit Sld5 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:16990792
Two-hybridPMID:23695164
rad4BRCT domain protein Rad4 Affinity Capture-WesternPMID:22718908
mcm6MCM complex subunit Mcm6 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:22433840
cdc23MCM-associated protein Mcm10 Two-hybridPMID:22433840
psf3GINS complex subunit Psf3 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:16990792
mcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:22433840
cdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:22433840
psf1GINS complex subunit Psf1 Affinity Capture-WesternPMID:22718908
Affinity Capture-WesternPMID:16990792
sld3DNA replication pre-initiation complex subunit Sld3 Two-hybridPMID:21593208
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
bir1survivin, Bir1 Dosage RescuePMID:16199877
chk1Chk1 protein kinase Synthetic Growth DefectPMID:15466421
psf3GINS complex subunit Psf3 Dosage RescuePMID:16990792
swi3replication fork protection complex subunit Swi3 Synthetic Growth DefectPMID:20176980
rad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:15466421
swi1replication fork protection complex subunit Swi1 Synthetic LethalityPMID:20176980
External References
Database Identifier Description
NBRP SPBC725.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC725.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC725.13c BioGRID Interaction Datasets
Expression Viewer SPBC725.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC725.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC725.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC725.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC725.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC725.13c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC725.13c Cell Cycle Data
GEO SPBC725.13c GEO profiles
PInt SPBC725.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC725.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC725.13c Fission yeast phenotypic data & analysis
SPD / RIKEN05/05F08Orfeome Localization Data
UniProtKB/SwissProtO94329DNA replication complex GINS protein psf2
ModBaseO94329Database of comparative protein structure models
STRINGO94329Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595493GINS complex subunit Psf2
RefSeq mRNANM_001021404972h- GINS complex subunit Psf2 (psf2), mRNA
European Nucleotide ArchiveCAA22185.1ENA Protein Mapping
UniParcUPI000006A3B6UniProt Archive

Literature for psf2

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015