psf2 (SPBC725.13c)


Gene Standard Namepsf2 Characterisation Statuspublished
Systematic IDSPBC725.13c Feature Typeprotein coding
Synonymsbsh3, dre13 Name DescriptionDefect in REplication
ProductGINS complex subunit Psf2 Product Size183aa, 21.34 kDa
Genomic Location Chromosome II, 1229239-1228148 (1092nt); CDS:1229027-1228476 (552nt)

Ensembl Gene Location
GO Biological Process

GO Slim Terms

DNA replication


Term NameCount
mitotic DNA replication73
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication initiation34
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationpsf2ΔNull1435
unnamed (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclepsf2ΔNull846
abnormal premeiotic DNA replicationpsf2-HBD (steroid hormone-binding domain fusion)Not specified21
inviable elongated vegetative cellpsf2ΔNull408
inviable vegetative cell, abnormal cell shape, normal cell sizepsf2ΔNull199
mitotic G2/M transition delaypsf2-HBD (steroid hormone-binding domain fusion)Not specified31
protein mislocalized to cytoplasmpsf2-HBD (steroid hormone-binding domain fusion)Not specified36
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1229239..1228148
mRNA1229239..1228148
5' UTR1229239..1229028PMID:21511999
CDS1229027..1228476
3' UTR1228475..1228148PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05916 Pfam IPR021151 GINS complex 53 159 4
PTHR12772 HMMPANTHER IPR007257 GINS complex, subunit Psf2 1 183 1
SSF160059 SuperFamily 11 71 3
SSF158573 SuperFamily 72 176 4
PIRSF028998 PIRSF IPR016906 GINS complex, subunit Psf2, subgroup 1 183 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.62 Da
Charge -13.50
Isoelectric point 4.30
Molecular weight 21.34 kDa
Number of residues 183
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
716.23during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.72during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withcdc23MCM-associated protein Mcm10 Two-hybridPMID:22433840
affinity capturescdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:22718908
affinity capturescdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:22433840
affinity capturesmcm4MCM complex subunit Mcm4/Cdc21 Affinity Capture-WesternPMID:22433840
affinity capturesmcm6MCM complex subunit Mcm6 Affinity Capture-WesternPMID:22718908
affinity capturesmcm6MCM complex subunit Mcm6 Affinity Capture-WesternPMID:22433840
affinity capturespsf1GINS complex subunit Psf1 Affinity Capture-WesternPMID:22718908
affinity capturespsf1GINS complex subunit Psf1 Affinity Capture-WesternPMID:16990792
affinity capturespsf3GINS complex subunit Psf3 Affinity Capture-WesternPMID:22718908
affinity capturespsf3GINS complex subunit Psf3 Affinity Capture-WesternPMID:16990792
affinity capturesrad4BRCT domain protein Rad4 Affinity Capture-WesternPMID:22718908
binds DNA-binding domain construct withsld3DNA replication pre-initiation complex subunit Sld3 Two-hybridPMID:21593208
affinity capturessld5GINS complex subunit Sld5 Affinity Capture-WesternPMID:22718908
affinity capturessld5GINS complex subunit Sld5 Affinity Capture-WesternPMID:16990792
binds activation domain construct withsld5GINS complex subunit Sld5 Two-hybridPMID:23695164
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuesbir1survivin, Bir1 Dosage RescuePMID:16199877
synthetic growth defect withchk1Chk1 protein kinase Synthetic Growth DefectPMID:15466421
overexpression rescuespsf3GINS complex subunit Psf3 Dosage RescuePMID:16990792
synthetic lethal withrad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:15466421
synthetic lethal withswi1replication fork protection complex subunit Swi1 Synthetic LethalityPMID:20176980
synthetic growth defect withswi3replication fork protection complex subunit Swi3 Synthetic Growth DefectPMID:20176980
External References
Database Identifier Description
NBRP SPBC725.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC725.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC725.13c BioGRID Interaction Datasets
Expression Viewer SPBC725.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC725.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC725.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC725.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC725.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC725.13c Transcriptome Viewer (Bähler Lab)
GEO SPBC725.13c GEO profiles
PInt SPBC725.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC725.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC725.13c Fission yeast phenotypic data & analysis
Cyclebase SPBC725.13c.1 Cell Cycle Data
SPD / RIKEN05/05F08Orfeome Localization Data
UniProtKB/SwissProtO94329DNA replication complex GINS protein psf2
ModBaseO94329Database of comparative protein structure models
STRINGO94329Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595493GINS complex subunit Psf2
RefSeq mRNANM_001021404972h- GINS complex subunit Psf2 (psf2), mRNA
European Nucleotide ArchiveCAA22185.1ENA Protein Mapping
UniParcUPI000006A3B6UniProt Archive

Literature for psf2

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015