dis2 (SPBC776.02c)


Gene Standard Namedis2 Characterisation Statuspublished
Systematic IDSPBC776.02c Feature Typeprotein coding
Synonymsbws1, sds1 Name Descriptiondefective in sister chromatid disjoining
Productserine/threonine protein phosphatase PP1 subfamily, Dis2 Product Size327aa, 37.64 kDa
Genomic Location Chromosome II, 3175376-3173165 (2212nt); CDS:3175098-3173719 (1380nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO:0004721phosphoprotein phosphatase activity38
has substrate chk1IDAPMID:14765108
GO:0004722protein serine/threonine phosphatase activityIDAPMID:217002915
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000226microtubule cytoskeleton organizationIGIcdc13PMID:3428262105
GO:0044773mitotic DNA damage checkpointIGIchk1PMID:1476510843
GO:0000070mitotic sister chromatid segregationIMPPMID:3409871131
GO:2000784positive regulation of establishment of cell polarity regulating cell shapeEXPPMID:245544325
GO:1903068positive regulation of protein localization to cell tip2
regulates pom1EXPPMID:24554432
regulates gef1EXPPMID:24554432
GO:0006470protein dephosphorylationIDAPMID:1476510834
GO:0006364rRNA processingISOSGD:S000000935GO_REF:0000024193
GO:0060629regulation of homologous chromosome segregationIMPPMID:25442981
GO:0007346regulation of mitotic cell cycleIGIcdc2PMID:7957097280
GO:0007165signal transductionNASGO_REF:0000051306
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:168233724204
GO:0005829cytosolIDAPMID:168233722319
GO:0005847mRNA cleavage and polyadenylation specificity factor complexIDAPMID:1461782215
GO:0005634nucleusIDAPMID:168233722737
IDAPMID:2544298
GO:0000164protein phosphatase type 1 complexISOSGD:S000000935PMID:25442984
IPItea4PMID:24554432
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationCell growth assaydis2-11Not specifiedPECO:0000006, PECO:0000201, PECO:0000102PMID:34098711337
FYPO:0001164normal growth on glucose carbon sourceCell growth assaydis2-11Not specifiedPECO:0000102, PECO:0000004PMID:12376568165
FYPO:0000964normal growth on thiabendazoleCell growth assaydis2-11Not specifiedPMID:34098718
FYPO:0001357normal vegetative cell population growthCell growth assaydis2+ (wild type)OverexpressionPECO:0000005PMID:2170029618
Cell growth assaydis2+ (wild type)OverexpressionPECO:0000004PMID:2170029
FYPO:0000097sensitive to caffeineCell growth assaydis2-11EndogenousPMID:254429899
Cell growth assaydis2-11Not specifiedPECO:0000201, PECO:0000004, PECO:0000227PMID:3409871
FYPO:0000080sensitive to coldCell growth assaydis2cs (R245Q)Not specifiedPMID:217002942
Cell growth assaydis2-11Not specifiedPECO:0000102PMID:12376568
FYPO:0002060viable vegetative cell populationMicroscopydis2ΔNullPMID:204732893751
Microscopydis2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000141abnormal mitotic sister chromatid segregationMicroscopydis2-11Not specifiedPECO:0000006PMID:3409871141
FYPO:0000671abnormal rDNA separationMicroscopydis2-11Not specifiedPECO:0000006PMID:34098713
FYPO:0001270complete but unequal mitotic sister chromatid separationMicroscopydis2-11EndogenousPECO:0000004PMID:25442987
FYPO:0001757decreased protein phosphatase activity4
expressivity FYPO_EXT:0000002Enzyme assay datadis2cs (R245Q)Not specifiedPECO:0000004PMID:2170029
expressivity FYPO_EXT:0000001Enzyme assay datadis2::ura4+ (disruption)Not specifiedPMID:2170029
expressivity FYPO_EXT:0000001Enzyme assay datadis2cs (R245Q)Not specifiedPECO:0000005PMID:2170029
FYPO:0001840increased minichromosome loss during vegetative growthMicroscopydis2-11Not specifiedPECO:0000004, PECO:0000227PMID:340987132
FYPO:0001862increased minichromosome loss upon segregation during meiotic cell cyclePlasmid maintenance assay evidencedis2-11EndogenousPECO:0000004PMID:25442981
FYPO:0001758increased protein phosphatase activityEnzyme assay datadis2+ (wild type)OverexpressionPMID:21700292
FYPO:0001761normal mitotic G1/S phase transitionFlow cytometry datadis2-11Not specifiedPECO:0000006PMID:19442663
FYPO:0000124viable cellCell growth assaydis2Δ::ura4+NullPMID:25442923606
FYPO:0001491viable vegetative cellCell growth assaydis2-11Not specifiedPECO:0000004PMID:25442983599
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopydis2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in prp4 serine/threonine protein kinase Prp4 PMID:10545451
FYPO affected by mutation in sds22 protein phosphatase regulatory subunit Sds22 PMID:1846086
FYPO affected by mutation in tea4 tip elongation aberrant protein Tea4 PMID:24554432
GO substrate of cdc2 cyclin-dependent protein kinase Cdk1/Cdc2 PMID:7957097
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
131753763175047
231746503173165
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00149 Pfam IPR004843 Phosphoesterase domain 58 249 20
SM00156 SMART IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 29 299 10
PS00125 Prosite Patterns IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 120 125 10
PTHR11668 HMMPANTHER 6 300 11
PTHR11668:SF97 HMMPANTHER 6 300 1
3.60.21.10 Gene3D 9 306 23
SSF56300 SuperFamily Metallo-dependent phosphatase-like 19 312 22
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 153 179 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 239 259 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 261 277 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 57 84 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 182 209 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 86 113 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 119 143 10

View domain organization at Pfam

Protein Properties

Ave. residue weight 115.10 Da
Charge -4.50
Isoelectric point 5.44
Molecular weight 37.64 kDa
Number of residues 327
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueNAST316PMID:79570971922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
25473during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
18066during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
35145during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
36382.83during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
13953.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae DIS2partial PMID:2544298
Subunit Composition
DescriptionQualifierReferenceCount
oligomeric PMID:21700294
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
htb1histone H2B Htb1 Synthetic Growth DefectPMID:16688222
hip1hira protein, histone chaperone Hip1 Synthetic Growth DefectPMID:21965289
dis1microtubule-associated protein Dis1 Synthetic LethalityPMID:1944266
rad2414-3-3 protein Rad24 Synthetic Growth DefectPMID:21965289
rsc1RSC complex subunit Rsc1 Synthetic Growth DefectPMID:21965289
spc7kinetochore protein blinkin, Spc7 Synthetic LethalityPMID:22521786
SPBC25D12.06ATP-dependent RNA helicase (predicted) Synthetic Growth DefectPMID:21965289
sds21serine/threonine protein phosphatase PP1 subfamily, Sds21 Synthetic LethalityPMID:2170029
Synthetic LethalityPMID:2544298
sds22protein phosphatase regulatory subunit Sds22 Phenotypic SuppressionPMID:1846086
vps32ESCRT III complex subunit Vps32 Synthetic Growth DefectPMID:21965289
saf1splicing associated factor Saf1 Synthetic Growth DefectPMID:21965289
cwf21complexed with Cdc5 protein Cwf21 Synthetic Growth DefectPMID:21965289
cdc7serine/threonine protein kinase Cdc7 Dosage LethalityPMID:24532715
Phenotypic Suppression
wee1M phase inhibitor protein kinase Wee1 Dosage RescuePMID:1944266
clp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic EnhancementPMID:21965289
dis33'-5' exoribonuclease subunit Dis3 (predicted) Synthetic LethalityPMID:1944266
bst1GPI inositol deacylase Bst1 (predicted) Synthetic Growth DefectPMID:21965289
ctf18RFC-like complex subunit Ctf18 Synthetic Growth DefectPMID:21965289
nse5Smc5-6 complex non-SMC subunit Nse5 Synthetic Growth DefectPMID:21965289
SPBC557.02cconserved fungal protein Synthetic Growth DefectPMID:21965289
SPBC336.13cmitochondrial inner membrane peptidase complex catalytic subunit 2 (predicted) Synthetic Growth DefectPMID:21965289
rhp18Rad18 homolog Rhp18 Synthetic Growth DefectPMID:14765108
nut2mediator complex subunit Med10 Synthetic Growth DefectPMID:21965289
plo1Polo kinase Plo1 Synthetic Growth DefectPMID:22375062
cho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Synthetic Growth DefectPMID:21965289
mis3rRNA processing protein Mis3 Synthetic RescuePMID:10947840
cdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic RescuePMID:23333317
Dosage RescuePMID:1944266
uge1UDP-glucose 4-epimerase Uge1 Synthetic Growth DefectPMID:21965289
ubr1N-end-recognizing protein Ubr1 Synthetic Growth DefectPMID:21965289
SPBC691.04mitochondrial ATP-dependent RNA helicase Mss116 (predicted) Synthetic Growth DefectPMID:21965289
smd3Sm snRNP core protein Smd3 Synthetic Growth DefectPMID:21965289
cut8tethering factor for nuclear proteasome Cut8 Synthetic LethalityPMID:11084332
sft1SNARE Sft1 (predicted) Synthetic Growth DefectPMID:21965289
arb2argonaute binding protein 2 Synthetic Growth DefectPMID:21965289
SPCC1494.08cconserved fungal protein Synthetic Growth DefectPMID:21965289
cuf1nutritional copper sensing transcription factor Cuf1 Synthetic Growth DefectPMID:21965289
cut12spindle pole body protein Cut12 Phenotypic SuppressionPMID:2245912
ppt1para-hydroxybenzoate--polyprenyltransferase Ppt1 Synthetic Growth DefectPMID:21965289
rpm1mitochondrial 3'-5' exonuclease for RNA 3' ss-tail Synthetic Growth DefectPMID:21965289
cox6cytochrome c oxidase subunit VI (predicted) Synthetic Growth DefectPMID:21965289
arp8actin-like protein, Ino80 complex subunit Arp8 Synthetic Growth DefectPMID:21965289
ark1aurora-B kinase Ark1 Synthetic RescuePMID:21965289
Phenotypic SuppressionPMID:23438755
alp31tubulin specific chaperone cofactor A, Alp31 Synthetic Growth DefectPMID:21965289
cut9anaphase-promoting complex subunit Cut9 Synthetic LethalityPMID:9264466
nse6Smc5-6 complex non-SMC subunit Nse6 Synthetic Growth DefectPMID:21965289
rhp41DNA repair protein Rhp41 Synthetic Growth DefectPMID:21965289
prf1RNA polymerase II associated Paf1 complex (predicted) Synthetic Growth DefectPMID:21965289
rad51RecA family recombinase Rad51/Rhp51 Synthetic Growth DefectPMID:21965289
Synthetic Growth DefectPMID:14765108
rif1telomere length regulator protein Rif1 Phenotypic SuppressionPMID:24532715
nsk1Clp1-interacting, microtubule plus-end binding Nsk1 Phenotypic EnhancementPMID:21965289
Synthetic Growth Defect
Synthetic Growth DefectPMID:22065639
cho2phosphatidylethanolamine N-methyltransferase Cho2 Synthetic Growth DefectPMID:21965289
coq5C-methyltransferase (predicted) Synthetic Growth DefectPMID:21965289
alp14TOG ortholog Alp14 Synthetic Growth DefectPMID:21965289
rad13DNA repair nuclease Rad13 Synthetic Growth DefectPMID:14765108
atp10F1-F0 ATPase assembly protein (predicted) Synthetic Growth DefectPMID:21965289
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Synthetic Growth DefectPMID:21965289
pub1HECT-type ubiquitin-protein ligase E3 Pub1 Synthetic Growth DefectPMID:21965289
dam1DASH complex subunit Dam1 Synthetic Growth DefectPMID:22375062
ned1lipin Ned1 Synthetic Growth DefectPMID:12376568
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
SPAC824.04WD repeat protein (predicted) Affinity Capture-MSPMID:14617822
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:16920624
Biochemical ActivityPMID:7957097
cut12spindle pole body protein Cut12 Affinity Capture-WesternPMID:23333317
Two-hybrid
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
pom1DYRK family protein kinase Pom1 FRETPMID:21703453
rif1telomere length regulator protein Rif1 Affinity Capture-WesternPMID:24656819
Two-hybridPMID:24532715
tea4tip elongation aberrant protein Tea4 FRETPMID:21703453
Affinity Capture-Western
Co-purificationPMID:24554432
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
ysh1mRNA cleavage and polyadenylation specificity factor complex endoribonuclease subunit Ysh1 Affinity Capture-MSPMID:14617822
External References
Database Identifier Description
NBRP SPBC776.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC776.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC776.02c BioGRID Interaction Datasets
Expression Viewer SPBC776.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC776.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC776.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC776.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC776.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC776.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC776.02c Cell Cycle Data
GEO SPBC776.02c GEO profiles
PInt SPBC776.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC776.02c Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz3.1.3.16Integrated relational Enzyme database
Rhea3.1.3.16Annotated reactions database
SPD / RIKEN40/40E03Orfeome Localization Data
UniProtKB/SwissProtP13681Serine/threonine-protein phosphatase PP1-1
ModBaseP13681Database of comparative protein structure models
STRINGP13681Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596317serine/threonine protein phosphatase PP1
RefSeq mRNANM_001022239972h- serine/threonine protein phosphatase PP1 (dis2), mRNA
European Nucleotide ArchiveAAA74731.1ENA Protein Mapping
European Nucleotide ArchiveAAA89197.1ENA Protein Mapping
European Nucleotide ArchiveCAA22875.1ENA Protein Mapping
UniParcUPI0000131FABUniProt Archive

Literature for dis2

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014