SPBC776.03


Gene Standard NameUnassigned Characterisation Statusbiological role inferred
Systematic IDSPBC776.03 Feature Typeprotein coding
Synonyms Name Description
Producthomoserine dehydrogenase (predicted) Product Size376aa, 40.04 kDa
Genomic Location Chromosome II, 3176669-3177967 (1299nt); CDS:3176694-3177893 (1200nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
homoserine dehydrogenase activity1
Annotation ExtensionEvidenceWith/FromReference
NADP binding19
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
homoserine biosynthetic process2
Annotation ExtensionEvidenceWith/FromReference
isoleucine biosynthetic process9
Annotation ExtensionEvidenceWith/FromReference
methionine biosynthetic process20
Annotation ExtensionEvidenceWith/FromReference
threonine biosynthetic process5
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationSPBC776.03Δ3844

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologySPBC776.03Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3176669..3176733, 3176803..3177967
Intron3176734..3176802
mRNA3176669..3177967
5' UTR3176669..3176693PMID:21511999
CDS3176694..3176733, 3176803..3177893
3' UTR3177894..3177967AU013567
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00742 Pfam IPR001342 Homoserine dehydrogenase, catalytic 158 362 1
PF03447 Pfam IPR005106 Aspartate/homoserine dehydrogenase, NAD-binding 14 149 1
TMhelix TMHMM 340 362 953
PS01042 Prosite Patterns IPR019811 Homoserine dehydrogenase, conserved site 209 231 1
PTHR21499:SF1 HMMPANTHER 8 367 1
PTHR21499 HMMPANTHER 8 367 2
3.40.50.720 Gene3D IPR016040 NAD(P)-binding domain 6 179 100
3.30.360.10 Gene3D Glucose-6-phosphate dehydrogenase 180 343 14
SSF51735 SuperFamily IPR016040 NAD(P)-binding domain 6 180 88
SSF55347 SuperFamily 158 349 12
PIRSF036497 PIRSF IPR022697 Homoserine dehydrogenase lacking ACT domain 5 370 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 106.48 Da
Charge -0.50
Codon Adaptation Index 0.62
Isoelectric point 6.38
Molecular weight 40.04 kDa
Number of residues 376
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during cellular response to thiabendazoleS201
present during mitotic M phaseS324
S324
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S201 PMID:18257517
present during mitotic M phase experimental evidence S324 PMID:21712547
experimental evidence S324 PMID:24763107
phosphorylated residue 1932
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK110, K124 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K110 PMID:26412298
mass spectrometry evidence K124 PMID:26412298
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for SPBC776.03

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
277627during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
284436during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
292345during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
288467during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
268996during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
98616.71during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
33343.92during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
23during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in eukaryotes4516
conserved in bacteria1002
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC776.03 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byfar8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPBC776.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC776.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC776.03 BioGRID Interaction Datasets
Expression Viewer SPBC776.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC776.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC776.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC776.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC776.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC776.03 Transcriptome Viewer (Bähler Lab)
GEO SPBC776.03 GEO profiles
PInt SPBC776.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC776.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC776.03 Fission yeast phenotypic data & analysis
Cyclebase SPBC776.03.1 Cell Cycle Data
IntEnz1.1.1.3Integrated relational Enzyme database
Rhea1.1.1.3Annotated reactions database
SPD / RIKEN19/19B11Orfeome Localization Data
UniProtKB/SwissProtO94671Probable homoserine dehydrogenase
ModBaseO94671Database of comparative protein structure models
STRINGO94671Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596318homoserine dehydrogenase (predicted)
RefSeq mRNANM_001022240972h- homoserine dehydrogenase (predicted) (SPBC776.03), mRNA
European Nucleotide ArchiveCAA22876.1ENA Protein Mapping
KEGG00260+1.1.1.3Glycine, serine and threonine metabolism
KEGG00270+1.1.1.3Cysteine and methionine metabolism
KEGG00300+1.1.1.3Lysine biosynthesis
UniParcUPI0000129356UniProt Archive
UniPathwayUPA00050Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5
UniPathwayUPA00051Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3

Literature for SPBC776.03

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016