ucp8 (SPBC83.01)


Gene Standard Nameucp8 Characterisation Statuspublished
Systematic IDSPBC83.01 Feature Typeprotein coding
Synonyms Name Description
ProductUBA/EH/EF hand domain protein Ucp8 Product Size829aa, 91.12 kDa
Genomic Location Chromosome II, 1510190-1513963 (3774nt); CDS:1510581-1513363 (2783nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium ion binding39
Annotation ExtensionEvidenceWith/FromReference
polyubiquitin binding11
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cortical patch assembly22
Annotation ExtensionEvidenceWith/FromReference
endocytosis77
Annotation ExtensionEvidenceWith/FromReference
intracellular protein transport316
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationucp8Δ3850

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyucp8Δ3102
Target Of
OntologyRelationshipGeneProduct
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1510190..1510730, 1510881..1511004, 1511050..1513235, 1513334..1513963
Intron1510731..1510880, 1511005..1511049, 1513236..1513333
mRNA1510190..1513963
5' UTR1510190..1510580PMID:21511999
CDS1510581..1510730, 1510881..1511004, 1511050..1513235, 1513334..1513363
3' UTR1513364..1513963PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12763 Pfam IPR000261 EH domain 301 365 4
PF12763 Pfam IPR000261 EH domain 156 225 4
PF00627 Pfam IPR015940 Ubiquitin-associated domain 789 825 8
SM00165 SMART IPR015940 Ubiquitin-associated domain 790 827 7
SM00027 SMART IPR000261 EH domain 122 224 5
SM00027 SMART IPR000261 EH domain 293 390 5
SM00027 SMART IPR000261 EH domain 3 89 5
PS50222 Prosite Profiles IPR002048 EF-hand domain 43 78 20
PS50030 Prosite Profiles IPR015940 Ubiquitin-associated domain 788 828 9
PS50222 Prosite Profiles IPR002048 EF-hand domain 333 368 20
PS50031 Prosite Profiles IPR000261 EH domain 300 386 5
PS50031 Prosite Profiles IPR000261 EH domain 10 99 5
PS50031 Prosite Profiles IPR000261 EH domain 129 225 5
PTHR11216 HMMPANTHER 1 261 6
PTHR11216 HMMPANTHER 280 390 6
PTHR11216 HMMPANTHER 791 800 6
PTHR11216:SF61 HMMPANTHER 1 261 2
PTHR11216:SF61 HMMPANTHER 280 390 2
PTHR11216:SF61 HMMPANTHER 791 800 2
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 3 101 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 107 224 20
1.10.8.10 Gene3D S-adenosyl-L-methionine-dependent methyltransferase 779 827 19
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 284 378 20
SSF47473 SuperFamily IPR011992 EF-hand domain pair 5 81 21
SSF47473 SuperFamily IPR011992 EF-hand domain pair 294 375 21
SSF47473 SuperFamily IPR011992 EF-hand domain pair 119 223 21
SSF46934 SuperFamily IPR009060 UBA-like 767 828 13
Coil ncoils Predicted coiled-coil protein (DUF2205) 688 708 1055
Coil ncoils Predicted coiled-coil protein (DUF2205) 636 669 1055
Coil ncoils Predicted coiled-coil protein (DUF2205) 604 624 1055

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276
PBO:0000794UBA domain proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000794
PBO:0003116EH domainsTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0003116

Term IDTerm NameReferenceCount
SO:0001080coiled_coil107
qualifier region

Protein Properties

Ave. residue weight 109.91 Da
Charge -41.00
Codon Adaptation Index 0.40
Isoelectric point 4.43
Molecular weight 91.12 kDa
Number of residues 829
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS259
present during mitotic M phaseS266
present during mitotic M phaseS541
present during mitotic M phaseS557
S111, S112, S113, S259, S260
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S259 PMID:21712547
present during mitotic M phase experimental evidence S266 PMID:21712547
present during mitotic M phase experimental evidence S541 PMID:21712547
present during mitotic M phase experimental evidence S557 PMID:21712547
IDA S111 PMID:25720772
IDA S112 PMID:25720772
IDA S260 PMID:25720772
IDA S259 PMID:25720772
IDA S113 PMID:25720772
O-phospho-L-threonine 1085
present during mitotic M phaseT547
T115
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T547 PMID:21712547
IDA T115 PMID:25720772
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK688 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K688 PMID:26412298
IDA PMID:26412298
O-phosphorylated residueS111,S112, S111,S113, S111,T115, S112,S113, S112,T115, S113,T115 2457
Annotation ExtensionEvidenceResidueReference
IDA S111,T115 PMID:25720772
IDA S111,S112 PMID:25720772
IDA S112,T115 PMID:25720772
IDA S113,T115 PMID:25720772
IDA S111,S113 PMID:25720772
IDA S112,S113 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ucp8 (SPBC83.01)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
19999during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
20790during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
18805during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
19045during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
19527during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6331.51during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
7714.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999435
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPBC83.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC83.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC83.01 BioGRID Interaction Datasets
Expression Viewer SPBC83.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC83.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC83.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC83.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC83.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC83.01 Transcriptome Viewer (Bähler Lab)
GEO SPBC83.01 GEO profiles
PInt SPBC83.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC83.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC83.01 Fission yeast phenotypic data & analysis
Cyclebase SPBC83.01.1 Cell Cycle Data
SPD / RIKEN29/29F01Orfeome Localization Data
UniProtKB/SwissProtO94685UBA domain-containing protein 8
ModBaseO94685Database of comparative protein structure models
STRINGO94685Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595633UBA/EH/EF hand domain protein Ucp8
RefSeq mRNANM_001021527972h- UBA/EH/EF hand domain protein Ucp8 (ucp8), mRNA
European Nucleotide ArchiveCAB36863.2ENA Protein Mapping
UniParcUPI000228F3B6UniProt Archive

Literature for ucp8

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016