ucp8 (SPBC83.01)


Gene Standard Nameucp8 Characterisation Statuspublished
Systematic IDSPBC83.01 Feature Typeprotein coding
Synonyms Name Description
ProductUBA/EH/EF hand domain protein Ucp8 Product Size829aa, 91.12 kDa
Genomic Location Chromosome II, 1510190-1513963 (3774nt); CDS:1510581-1513363 (2783nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium ion binding48
Annotation ExtensionEvidenceWith/FromReference
polyubiquitin binding11
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cortical patch assembly22
Annotation ExtensionEvidenceWith/FromReference
endocytosis76
Annotation ExtensionEvidenceWith/FromReference
intracellular protein transport338
Annotation ExtensionEvidenceWith/FromReference
receptor-mediated endocytosis2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyucp8ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopyucp8ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyucp8ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
115101901510730
215108811511004
315110501513235
415133341513963

UTRs

Region Coordinates Reference
five_prime_UTR1510190..1510580PMID:21511999
three_prime_UTR1513364..1513963PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12763 Pfam 156 225 4
PF12763 Pfam 301 365 4
PF00627 Pfam IPR000449 Ubiquitin-associated domain/translation elongation factor EF-Ts, N-terminal 789 825 9
SM00027 SMART IPR000261 EPS15 homology (EH) 293 390 5
SM00027 SMART IPR000261 EPS15 homology (EH) 3 89 5
SM00027 SMART IPR000261 EPS15 homology (EH) 122 224 5
SM00165 SMART IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote 790 827 7
PS50031 Prosite Profiles IPR000261 EPS15 homology (EH) 10 99 5
PS50222 Prosite Profiles IPR002048 EF-hand domain 333 368 20
PS50030 Prosite Profiles IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote 788 828 9
PS50031 Prosite Profiles IPR000261 EPS15 homology (EH) 129 225 5
PS50031 Prosite Profiles IPR000261 EPS15 homology (EH) 300 386 5
PS50222 Prosite Profiles IPR002048 EF-hand domain 43 78 20
PTHR11216 HMMPANTHER 3 801 6
PTHR11216:SF46 HMMPANTHER 3 801 2
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 284 378 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 3 101 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 107 224 20
1.10.8.10 Gene3D 779 827 19
SSF47473 SuperFamily 294 375 21
SSF47473 SuperFamily 5 81 21
SSF47473 SuperFamily 119 223 21
SSF46934 SuperFamily IPR009060 UBA-like 767 828 13
Coil ncoils Rabaptin coiled-coil domain 604 625 968
Coil ncoils Rabaptin coiled-coil domain 636 670 968
Coil ncoils Rabaptin coiled-coil domain 688 709 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276
PBO:0000794UBA domain proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000794
PBO:0003116EH domainsTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0003116

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001080coiled_coil106

Protein Properties

Ave. residue weight 109.91 Da
Charge -41.00
Isoelectric point 4.43
Molecular weight 91.12 kDa
Number of residues 829
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS541PMID:217125471670
present during mitotic M phase
present during mitotic M phaseexperimental evidenceS266PMID:21712547
present during mitotic M phaseexperimental evidenceS259PMID:21712547
present during mitotic M phaseexperimental evidenceS557PMID:21712547
MOD:00047O-phospho-L-threonineexperimental evidenceT547PMID:21712547692
present during mitotic M phase
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
19999during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
20790during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
18805during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
19045during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6331.51during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
19527during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7714.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.6during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPBC83.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC83.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC83.01 BioGRID Interaction Datasets
Expression Viewer SPBC83.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC83.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC83.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC83.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC83.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC83.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBC83.01 Cell Cycle Data
GEO SPBC83.01 GEO profiles
PInt SPBC83.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC83.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC83.01 Fission yeast phenotypic data & analysis
SPD / RIKEN29/29F01Orfeome Localization Data
UniProtKB/SwissProtO94685UBA domain-containing protein 8
ModBaseO94685Database of comparative protein structure models
STRINGO94685Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595633UBA/EH/EF hand domain protein Ucp8
RefSeq mRNANM_001021527972h- UBA/EH/EF hand domain protein Ucp8 (ucp8), mRNA
European Nucleotide ArchiveCAB36863.2ENA Protein Mapping
UniParcUPI000228F3B6UniProt Archive

Literature for ucp8

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014