rpl803 (SPBC839.04)


Gene Standard Namerpl803 Characterisation Statusbiological role inferred
Systematic IDSPBC839.04 Feature Typeprotein coding
Synonymsrpk5-b, rpkD4, rpl8-3, SPBC24E9.04 Name Description
Product60S ribosomal protein L8 (predicted) Product Size253aa, 27.10 kDa
Genomic Location Chromosome II, 601209-602186 (978nt); CDS:601281-602042 (762nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
rRNA binding57
Annotation ExtensionEvidenceWith/FromReference
structural constituent of ribosome257
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation470
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
cytosolic large ribosomal subunit83
Annotation ExtensionEvidenceWith/FromReference
nucleus2691
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
sensitive to bortezomibrpl803Δ255
viable vegetative cell populationrpl803Δ3844

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyrpl803Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons601209..602186
mRNA601209..602186
5' UTR601209..601280PMID:21511999
CDS601281..602042
3' UTR602043..602186PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03947 Pfam IPR022669 Ribosomal protein L2, C-terminal 97 221 4
PF00181 Pfam IPR022666 Ribosomal Proteins L2, RNA binding domain 15 89 4
PS00467 Prosite Patterns IPR022671 Ribosomal protein L2, conserved site 196 207 4
PTHR13691 HMMPANTHER IPR002171 Ribosomal protein L2 1 253 4
PTHR13691:SF16 HMMPANTHER 1 253 3
4.10.950.10 Gene3D IPR014726 Ribosomal protein L2, domain 3 169 243 4
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 1 85 48
2.30.30.30 Gene3D IPR014722 Ribosomal protein L2 domain 2 89 168 13
SSF50104 SuperFamily IPR008991 Translation protein SH3-like domain 98 244 16
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 2 96 57
PIRSF002158 PIRSF IPR002171 Ribosomal protein L2 1 251 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.12 Da
Charge 29.50
Codon Adaptation Index 0.73
Isoelectric point 11.45
Molecular weight 27.10 kDa
Number of residues 253
Modifications

Protein Modifications

Term NameResidueCount
ubiquitinylated lysineK144, K180 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K144 PMID:26412298
mass spectrometry evidence K180 PMID:26412298
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for rpl803 (SPBC839.04)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
52during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in eukaryotes4516
conserved in bacteria1002
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBC839.04 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withsec18secretory pathway protein Sec18 (predicted) Two-hybridPMID:26771498
binds DNA-binding domain construct withnap2nucleosome assembly protein Nap2 Two-hybridPMID:26771498
affinity captured bynak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
affinity captured bycid14poly(A) polymerase Cid14 Affinity Capture-MSPMID:20403971
affinity captured byair1zinc knuckle TRAMP complex subunit Air1 Affinity Capture-MSPMID:20403971
affinity captured byimp1importin alpha Affinity Capture-MSPMID:25963819
External References
Database Identifier Description
NBRP SPBC839.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBC839.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBC839.04 BioGRID Interaction Datasets
Expression Viewer SPBC839.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBC839.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBC839.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBC839.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBC839.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBC839.04 Transcriptome Viewer (Bähler Lab)
GEO SPBC839.04 GEO profiles
PInt SPBC839.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBC839.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBC839.04 Fission yeast phenotypic data & analysis
Cyclebase SPBC839.04.1 Cell Cycle Data
SPD / RIKEN09/09D05Orfeome Localization Data
UniProtKB/SwissProtP0CT7260S ribosomal protein L2-C
ModBaseP0CT72Database of comparative protein structure models
STRINGP0CT72Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59524460S ribosomal protein L2
RefSeq mRNANM_001021150972h- 60S ribosomal protein L2 (rpl803), mRNA
European Nucleotide ArchiveBAA87149.1ENA Protein Mapping
European Nucleotide ArchiveCAA34428.1ENA Protein Mapping
European Nucleotide ArchiveCAB46697.1ENA Protein Mapping
UniParcUPI0000133ED3UniProt Archive

Literature for rpl803

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016