orc4 (SPBP23A10.13)


Gene Standard Nameorc4 Characterisation Statuspublished
Systematic IDSPBP23A10.13 Feature Typeprotein coding
Synonymsorp4 Name Description
Productorigin recognition complex subunit Orc4 Product Size972aa, 108.61 kDa
Genomic Location Chromosome II, 2025363-2028676 (3314nt); CDS:2025543-2028461 (2919nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0016887ATPase activityNASGO_REF:0000051232
GO:0003688DNA replication origin bindingIDAPMID:117174258
IDAPMID:10077566
IDAPMID:12374757
IDAPMID:11689699
IDAPMID:11850415
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1902975DNA replication initiation involved in mitotic DNA replicationICGO:0005664PMID:1053592829
GO:1902597positive regulation of DNA replication origin binding1
regulates cdc18IDAPMID:11850415
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031261DNA replication preinitiation complexICGO:0005664PMID:1053592821
GO:0005664nuclear origin of replication recognition complexIDAPMID:105359286
IDAPMID:10535928
IDAPMID:11717425
IDAPMID:11689699
IDAPMID:11850415
GO:0005656nuclear pre-replicative complexICGO:0005664PMID:1053592814
GO:0043596nuclear replication forkICGO:0005664PMID:1053592864
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyorc4Δ::ura4+NullPMID:100775661337
Microscopyorc4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopyorc4ΔNullPMID:20473289
Cell growth assayorc4ΔN (1-435)Not specifiedPMID:11850415

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000158DNA content increased during vegetative growth15
penetrance FYPO_EXT:0000002Flow cytometry dataorc4+ (wild type)OverexpressionPMID:10077566
penetrance FYPO_EXT:0000002Flow cytometry dataorc4ΔN (1-435)OverexpressionPMID:10077566
FYPO:0000059abnormal mitotic cell cycleMicroscopyorc4ΔNullPECO:0000005, PECO:0000137PMID:23697806640
FYPO:0000141abnormal mitotic sister chromatid segregationFlow cytometry datanot recorded (487-972)OverexpressionPMID:10077566141
FYPO:0000062abnormal nuclear morphology during vegetative growthMicroscopyorc4+ (wild type)OverexpressionPMID:1007756646
Microscopyorc4ΔN (1-435)OverexpressionPMID:10077566
Microscopyorc4Δ::ura4+NullPMID:10077566
FYPO:0001095abolished DNA replication origin bindingOthernot recorded (1-515)Not specifiedPMID:117174253
FYPO:0003165cutMicroscopynot recorded (487-972)OverexpressionPMID:1007756680
Microscopyorc4ΔN (1-435)OverexpressionPMID:10077566
Microscopyorc4+ (wild type)OverexpressionPMID:10077566
Microscopyorc4Δ::ura4+NullPMID:10077566
FYPO:0002281inviable after spore germination with abnormal germ tube morphologyMicroscopyorc4ΔNullPECO:0000004, PECO:0000137PMID:23697806165
FYPO:0000839inviable elongated mononucleate aseptate cellMicroscopyorc4Δ::ura4+NullPMID:1007756630
Microscopyorc4+ (wild type)OverexpressionPMID:10077566
FYPO:0001490inviable elongated vegetative cell389
penetrance FYPO_EXT:0000003Microscopyorc4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopyorc4Δ::ura4+NullPMID:10077566
FYPO:0002273inviable vegetative cell with abnormal cell morphologyMicroscopyorc4ΔNullPECO:0000005, PECO:0000137PMID:23697806740
FYPO:0002974normal origin recognition complex assemblyGel electrophoresis evidencenot recorded (1-515)Not specifiedPMID:117174251
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
120253632028676
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 364 374 1
PF14629 Pfam 754 951 1
PF13191 Pfam 552 716 2
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 339 346 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 123 133 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 100 111 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 231 240 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 310 321 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 201 212 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 425 435 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 148 156 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 123 135 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 201 213 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 231 243 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 339 351 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 100 112 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 310 322 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 148 160 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 364 376 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 425 437 1
SM00382 SMART IPR003593 AAA+ ATPase domain 574 747 70
PTHR12087 HMMPANTHER 162 972 1
PTHR12087:SF0 HMMPANTHER 162 972 1
3.40.50.300 Gene3D 553 741 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 674 779 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 552 636 294
PR00929 PRINTS IPR020478 AT hook-like 123 133 1
PR00929 PRINTS IPR020478 AT hook-like 199 210 1
PR00929 PRINTS IPR020478 AT hook-like 229 239 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539
PBO:0002147AT hook proteinPMID:10077566Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002147

Protein Properties

Ave. residue weight 111.74 Da
Charge 31.00
Isoelectric point 9.79
Molecular weight 108.61 kDa
Number of residues 972
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS223PMID:247631071670
S503PMID:24763107
present during cellular response to thiabendazoleIDAS503PMID:18257517
present during mitotic M phaseS503PMID:21712547
present during mitotic M phaseS406PMID:21712547
present during mitotic M phaseS420PMID:21712547
present during mitotic M phaseS223PMID:21712547
present during mitotic M phaseS519PMID:21712547
present during mitotic M phaseS531PMID:21712547
present during mitotic M phaseS4PMID:21712547
MOD:00047O-phospho-L-threonineT384PMID:21712547690
present during mitotic M phase
present during mitotic M phaseT535PMID:21712547
present during mitotic M phaseT405PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
Orc4 determines sequence specificity of ORC binding to DNA replication origins PMID:116896991
Orc4 binds to the T-rich strand of replication origins throughout the cell cycle PMID:123747571
Orc4 binding footprint at replication origins is extended in G1 in the presence of Cdc1 and the MCM complex PMID:123747571
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
orc5origin recognition complex subunit Orc5 Reconstituted ComplexPMID:11717425
Reconstituted ComplexPMID:18723846
Reconstituted ComplexPMID:10535928
Reconstituted ComplexPMID:11850415
orc1origin recognition complex subunit Orc1 Reconstituted ComplexPMID:11689699
Reconstituted ComplexPMID:11717425
Reconstituted ComplexPMID:12840006
Affinity Capture-WesternPMID:11683912
Reconstituted ComplexPMID:11850415
Reconstituted ComplexPMID:10535928
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
cdc18MCM loader Reconstituted ComplexPMID:11850415
orc3origin recognition complex subunit Orp3 Reconstituted ComplexPMID:11717425
Reconstituted ComplexPMID:10535928
Reconstituted ComplexPMID:11850415
orc2origin recognition complex subunit Orc2 Reconstituted ComplexPMID:11689699
Reconstituted ComplexPMID:11717425
Co-purification
Reconstituted ComplexPMID:12840006
Affinity Capture-WesternPMID:11683912
Reconstituted ComplexPMID:11850415
Reconstituted ComplexPMID:10535928
orc6origin recognition complex subunit Orc6 Reconstituted ComplexPMID:11717425
Reconstituted ComplexPMID:11689699
Reconstituted ComplexPMID:10535928
Reconstituted ComplexPMID:11850415
External References
Database Identifier Description
NBRP SPBP23A10.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBP23A10.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBP23A10.13 BioGRID Interaction Datasets
Expression Viewer SPBP23A10.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBP23A10.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBP23A10.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBP23A10.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBP23A10.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBP23A10.13 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBP23A10.13 Cell Cycle Data
GEO SPBP23A10.13 GEO profiles
PInt SPBP23A10.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBP23A10.13 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN34/34A02Orfeome Localization Data
UniProtKB/SwissProtQ9Y794Origin recognition complex subunit 4
ModBaseQ9Y794Database of comparative protein structure models
STRINGQ9Y794Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595825origin recognition complex subunit Orc4
RefSeq mRNANM_001021729972h- origin recognition complex subunit Orc4 (orc4), mRNA
European Nucleotide ArchiveAF125185ENA EMBL mapping
European Nucleotide ArchiveCAB66441.1ENA Protein Mapping
MetaCycPWY-1042Glycolysis IV (plant cytosol)
MetaCycPWY-5484Glycolysis II (from fructose-6P)
MetaCycPWY-6901Glucose and xylose degradation
MetaCycPWY-7003Glycerol degradation to butanol
UniParcUPI0000130E60UniProt Archive
UniPathwayUPA00109Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5

Literature for orc4

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014