orc4 (SPBP23A10.13)


Gene Standard Nameorc4 Characterisation Statuspublished
Systematic IDSPBP23A10.13 Feature Typeprotein coding
Synonymsorp4 Name Description
Productorigin recognition complex subunit Orc4 Product Size972aa, 108.61 kDa
Genomic Location Chromosome II, 2025363-2028676 (3314nt); CDS:2025543-2028461 (2919nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process

GO Slim Terms

DNA replication


Term NameCount
DNA topological change4
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication initiation31
Annotation ExtensionEvidenceWith/FromReference
positive regulation of DNA replication origin binding1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
DNA replication preinitiation complex18
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin358
Annotation ExtensionEvidenceWith/FromReference
nuclear origin of replication recognition complex6
nuclear pre-replicative complex14
Annotation ExtensionEvidenceWith/FromReference
nuclear replication fork61
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationorc4Δ1453
orc4ΔN (1-435)
orc4Δ::ura4+

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cycleorc4Δ858
abnormal mitotic sister chromatid segregation (487-972)268
abnormal nuclear morphology during vegetative growthorc4Δ::ura4+75
orc4+
orc4ΔN (1-435)
abolished DNA replication origin binding (1-515)3
cut (487-972)125
orc4ΔN (1-435)
orc4Δ::ura4+
orc4+
DNA content increased during vegetative growthorc4ΔN (1-435)22
orc4+
inviable after spore germination with abnormal germ tube morphologyorc4Δ166
inviable elongated mononucleate aseptate cellorc4+40
orc4Δ::ura4+
inviable elongated vegetative cellorc4Δ::ura4+416
orc4Δ
inviable vegetative cell with abnormal cell morphologyorc4Δ372
normal origin recognition complex assembly (1-515)1
Target Of
OntologyRelationshipGeneProduct
GO substrate of hsk1 Dbf4(Dfp1)-dependent protein kinase Hsk1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2025363..2028676
5' UTR2025363..2025542PMID:21511999
3' UTR2028462..2028676PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14629 Pfam IPR032705 Origin recognition complex subunit 4, C-terminal 754 949 1
PF13191 Pfam 552 716 3
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 123 134 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 201 212 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 148 156 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 310 321 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 425 435 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 364 374 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 231 241 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 339 346 1
SM00382 SMART IPR003593 AAA+ ATPase domain 574 747 70
SM00384 SMART IPR017956 AT hook, DNA-binding motif 310 322 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 123 135 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 148 160 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 339 351 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 100 112 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 231 243 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 364 376 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 425 437 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 201 213 1
PTHR12087 HMMPANTHER 106 368 1
PTHR12087 HMMPANTHER 464 972 1
PTHR12087:SF0 HMMPANTHER IPR016527 Origin recognition complex subunit 4 106 368 1
PTHR12087:SF0 HMMPANTHER IPR016527 Origin recognition complex subunit 4 464 972 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 553 741 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 552 636 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 674 779 294
PR00929 PRINTS IPR020478 AT hook-like 229 239 1
PR00929 PRINTS IPR020478 AT hook-like 123 133 1
PR00929 PRINTS IPR020478 AT hook-like 199 210 1

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539
PBO:0002147AT hook proteinPMID:10077566Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002147

Protein Properties

Ave. residue weight 111.74 Da
Charge 31.00
Codon Adaptation Index 0.34
Isoelectric point 9.79
Molecular weight 108.61 kDa
Number of residues 972
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS223
present during mitotic M phaseS531
present during mitotic M phaseS4
present during mitotic M phaseS519
present during mitotic M phaseS420
present during cellular response to thiabendazole, mitotic M phaseS503
present during mitotic M phaseS406
S223, S503
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S223 PMID:21712547
present during mitotic M phase experimental evidence S531 PMID:21712547
present during mitotic M phase experimental evidence S4 PMID:21712547
present during mitotic M phase experimental evidence S519 PMID:21712547
present during mitotic M phase experimental evidence S420 PMID:21712547
present during mitotic M phase experimental evidence S503 PMID:21712547
present during cellular response to thiabendazole IDA S503 PMID:18257517
present during mitotic M phase experimental evidence S406 PMID:21712547
experimental evidence S503 PMID:24763107
experimental evidence S223 PMID:24763107
O-phospho-L-threonine 700
present during mitotic M phaseT384
present during mitotic M phaseT535
present during mitotic M phaseT405
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T384 PMID:21712547
present during mitotic M phase experimental evidence T535 PMID:21712547
present during mitotic M phase experimental evidence T405 PMID:21712547
sumoylated lysine 173
Annotation ExtensionEvidenceResidueReference
IDA PMID:26404184
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelconstant during GO:0000278Western blot evidencePMID:10077566

Quantitative Gene Expression

View graphical display of gene expression data for orc4 (SPBP23A10.13)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
Orc4 determines sequence specificity of ORC binding to DNA replication origins PMID:116896991
Orc4 binds to the T-rich strand of replication origins throughout the cell cycle PMID:123747571
Orc4 binding footprint at replication origins is extended in G1 in the presence of Cdc1 and the MCM complex PMID:123747571
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBP23A10.13 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withorc3origin recognition complex subunit Orp3 Reconstituted ComplexPMID:11850415
forms complex withorc5origin recognition complex subunit Orc5 Reconstituted ComplexPMID:18723846
forms complex withorc2origin recognition complex subunit Orc2 Reconstituted ComplexPMID:10535928
forms complex withorc1origin recognition complex subunit Orc1 Reconstituted ComplexPMID:10535928
forms complex withorc6origin recognition complex subunit Orc6 Reconstituted ComplexPMID:10535928
forms complex withcdc18MCM loader Reconstituted ComplexPMID:11850415
affinity captured byorc1origin recognition complex subunit Orc1 Affinity Capture-WesternPMID:11683912
affinity captured byorc2origin recognition complex subunit Orc2 Affinity Capture-WesternPMID:11683912
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
co-purifies withorc2origin recognition complex subunit Orc2 Co-purificationPMID:11717425
External References
Database Identifier Description
NBRP SPBP23A10.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBP23A10.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBP23A10.13 BioGRID Interaction Datasets
Expression Viewer SPBP23A10.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBP23A10.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBP23A10.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBP23A10.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBP23A10.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBP23A10.13 Transcriptome Viewer (Bähler Lab)
GEO SPBP23A10.13 GEO profiles
PInt SPBP23A10.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBP23A10.13 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBP23A10.13 Fission yeast phenotypic data & analysis
Cyclebase SPBP23A10.13.1 Cell Cycle Data
SPD / RIKEN34/34A02Orfeome Localization Data
UniProtKB/SwissProtQ9Y794Origin recognition complex subunit 4
ModBaseQ9Y794Database of comparative protein structure models
STRINGQ9Y794Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595825origin recognition complex subunit Orc4
RefSeq mRNANM_001021729972h- origin recognition complex subunit Orc4 (orc4), mRNA

Literature for orc4

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016