orc4 (SPBP23A10.13)


Gene Standard Nameorc4 Characterisation Statuspublished
Systematic IDSPBP23A10.13 Feature Typeprotein coding
Synonymsorp4 Name Description
Productorigin recognition complex subunit Orc4 Product Size972aa, 108.61 kDa
Genomic Location Chromosome II, 2025363-2028676 (3314nt); CDS:2025543-2028461 (2919nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037557
GO:0016887ATPase activityNASGO_REF:0000051232
GO:0003688DNA replication origin bindingIDAPMID:100775668
IDAPMID:11689699
IDAPMID:11850415
IDAPMID:11717425
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1902975DNA replication initiation involved in mitotic DNA replicationICGO:0005664PMID:1053592829
GO:1902597positive regulation of DNA replication origin binding1
regulates cdc18IDAPMID:11850415
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031261DNA replication preinitiation complexICGO:0005664PMID:1053592821
GO:0005664nuclear origin of replication recognition complexIDAPMID:105359286
IDAPMID:11717425
IDAPMID:10535928
IDAPMID:11850415
IDAPMID:11689699
GO:0005656nuclear pre-replicative complexICGO:0005664PMID:1053592814
GO:0043596nuclear replication forkICGO:0005664PMID:1053592864
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationCell growth assayorc4ΔN (1-435)Not specifiedPMID:118504151331
Microscopyorc4Δ::ura4+NullPMID:10077566
Microscopyorc4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopyorc4ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000158DNA content increased during vegetative growth14
penetrance FYPO_EXT:0000002Flow cytometry dataorc4+ (wild type)OverexpressionPMID:10077566
penetrance FYPO_EXT:0000002Flow cytometry dataorc4ΔN (1-435)OverexpressionPMID:10077566
FYPO:0000059abnormal mitotic cell cycleMicroscopyorc4ΔNullPECO:0000005, PECO:0000137PMID:23697806638
FYPO:0000141abnormal mitotic sister chromatid segregationFlow cytometry datanot recorded (487-972)OverexpressionPMID:10077566140
FYPO:0000062abnormal nuclear morphology during vegetative growthMicroscopyorc4ΔN (1-435)OverexpressionPMID:1007756645
Microscopyorc4Δ::ura4+NullPMID:10077566
Microscopyorc4+ (wild type)OverexpressionPMID:10077566
FYPO:0001095abolished DNA replication origin bindingOthernot recorded (1-515)Not specifiedPMID:117174252
FYPO:0003165cutMicroscopyorc4Δ::ura4+NullPMID:1007756680
Microscopynot recorded (487-972)OverexpressionPMID:10077566
Microscopyorc4+ (wild type)OverexpressionPMID:10077566
Microscopyorc4ΔN (1-435)OverexpressionPMID:10077566
FYPO:0002281inviable after spore germination with abnormal germ tube morphologyMicroscopyorc4ΔNullPECO:0000004, PECO:0000137PMID:23697806165
FYPO:0000839inviable elongated mononucleate aseptate cellMicroscopyorc4Δ::ura4+NullPMID:1007756628
Microscopyorc4+ (wild type)OverexpressionPMID:10077566
FYPO:0001490inviable elongated vegetative cell385
penetrance FYPO_EXT:0000003Microscopyorc4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopyorc4Δ::ura4+NullPMID:10077566
FYPO:0002273inviable vegetative cell with abnormal cell morphologyMicroscopyorc4ΔNullPECO:0000005, PECO:0000137PMID:23697806737
FYPO:0002974normal origin recognition complex assemblyGel electrophoresis evidencenot recorded (1-515)Not specifiedPMID:117174251
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
120253632028676

UTRs

Region Coordinates Reference
five_prime_UTR2025363..2025542PMID:21511999
three_prime_UTR2028462..2028676PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 148 156 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 339 346 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 123 133 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 100 111 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 231 240 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 310 321 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 201 212 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 425 435 1
PF02178 Pfam IPR017956 AT hook, DNA-binding motif 364 374 1
PF14629 Pfam 754 951 1
PF13191 Pfam 552 716 2
SM00384 SMART IPR017956 AT hook, DNA-binding motif 123 135 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 201 213 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 231 243 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 339 351 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 100 112 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 310 322 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 364 376 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 148 160 1
SM00384 SMART IPR017956 AT hook, DNA-binding motif 425 437 1
SM00382 SMART IPR003593 AAA+ ATPase domain 574 747 70
PTHR12087 HMMPANTHER 174 972 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 553 741 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 674 779 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 552 636 294
PR00929 PRINTS IPR020478 AT hook-like 123 133 1
PR00929 PRINTS IPR020478 AT hook-like 199 210 1
PR00929 PRINTS IPR020478 AT hook-like 229 239 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539
PBO:0002147AT hook proteinPMID:10077566Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002147

Protein Properties

Ave. residue weight 111.74 Da
Charge 31.00
Isoelectric point 9.79
Molecular weight 108.61 kDa
Number of residues 972
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS503PMID:182575171669
present during cellular response to thiabendazole
S503PMID:24763107
present during mitotic M phaseS420PMID:21712547
present during mitotic M phaseS223PMID:21712547
present during mitotic M phaseS503PMID:21712547
present during mitotic M phaseS406PMID:21712547
present during mitotic M phaseS4PMID:21712547
S223PMID:24763107
present during mitotic M phaseS519PMID:21712547
present during mitotic M phaseS531PMID:21712547
MOD:00047O-phospho-L-threonineT535PMID:21712547689
present during mitotic M phase
present during mitotic M phaseT384PMID:21712547
present during mitotic M phaseT405PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171921
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
Orc4 determines sequence specificity of ORC binding to DNA replication origins PMID:116896991
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
orc5origin recognition complex subunit Orc5 Reconstituted ComplexPMID:11717425
Reconstituted ComplexPMID:18723846
Reconstituted ComplexPMID:10535928
Reconstituted ComplexPMID:11850415
orc1origin recognition complex subunit Orc1 Reconstituted ComplexPMID:11689699
Reconstituted ComplexPMID:11717425
Reconstituted ComplexPMID:12840006
Affinity Capture-WesternPMID:11683912
Reconstituted ComplexPMID:11850415
Reconstituted ComplexPMID:10535928
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
cdc18MCM loader Reconstituted ComplexPMID:11850415
orc3origin recognition complex subunit Orp3 Reconstituted ComplexPMID:11717425
Reconstituted ComplexPMID:10535928
Reconstituted ComplexPMID:11850415
orc2origin recognition complex subunit Orc2 Reconstituted ComplexPMID:11689699
Reconstituted ComplexPMID:11717425
Co-purification
Reconstituted ComplexPMID:12840006
Affinity Capture-WesternPMID:11683912
Reconstituted ComplexPMID:11850415
Reconstituted ComplexPMID:10535928
orc6origin recognition complex subunit Orc6 Reconstituted ComplexPMID:11717425
Reconstituted ComplexPMID:11689699
Reconstituted ComplexPMID:10535928
Reconstituted ComplexPMID:11850415
External References
Database Identifier Description
NBRP SPBP23A10.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBP23A10.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBP23A10.13 BioGRID Interaction Datasets
Expression Viewer SPBP23A10.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBP23A10.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBP23A10.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBP23A10.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBP23A10.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBP23A10.13 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBP23A10.13 Cell Cycle Data
GEO SPBP23A10.13 GEO profiles
PInt SPBP23A10.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBP23A10.13 Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2541298origin recognition complex subunit Orc4
EntrezGene2541298origin recognition complex subunit Orc4
SPD / RIKEN34/34A02Orfeome Localization Data
UniProtKB/SwissProtQ9Y794Origin recognition complex subunit 4
ModBaseQ9Y794Database of comparative protein structure models
STRINGQ9Y794Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_595825origin recognition complex subunit Orc4
RefSeq mRNANM_001021729972h- origin recognition complex subunit Orc4 (orc4), mRNA
European Nucleotide ArchiveAF125185ENA EMBL mapping
European Nucleotide ArchiveAAD21618ENA Protein Mapping
European Nucleotide ArchiveCAB66441ENA Protein Mapping
European Nucleotide ArchiveCAB66441.1ENA Protein Mapping
ReactomeREACT_205584
ReactomeREACT_209856
ReactomeREACT_222044
UniParcUPI0000130E60UniProt Archive

Literature for orc4

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014