ppb1 (SPBP4H10.04)


Gene Standard Nameppb1 Characterisation Statuspublished
Systematic IDSPBP4H10.04 Feature Typeprotein coding
SynonymsSPBC1346.01c Name Description
Productcalcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Product Size554aa, 63.71 kDa
Genomic Location Chromosome II, 2873842-2876109 (2268nt); CDS:2873997-2875859 (1863nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004723calcium-dependent protein serine/threonine phosphatase activityIMPPMID:169289591
GO:0005516calmodulin bindingIEAUniProtKB-KW:KW-0112GO_REF:000003710
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO:0016791phosphatase activity72
has substrate prz1IDAPMID:15657058
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0019722calcium-mediated signalingIMPPMID:169289596
GO:0030644cellular chloride ion homeostasisIMPPMID:94277482
GO:0071472cellular response to salt stressIMPPMID:2181160719
IMPPMID:9427748
GO:0051480cytosolic calcium ion homeostasisIMPPMID:218116075
GO:0071852fungal-type cell wall organization or biogenesisIGIits8PMID:1095095100
GO:0010523negative regulation of calcium ion transport into cytosolIMPPMID:218116072
GO:0006470protein dephosphorylationICGO:0004721GO_REF:000000134
GO:0031137regulation of conjugation with cellular fusionIMPPMID:798314258
GO:2000769regulation of establishment or maintenance of cell polarity regulating cell shapeIMPPMID:798314226
GO:1902412regulation of mitotic cytokinesisIGIits8PMID:1095095833
requiredIMPPMID:7983142
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005955calcineurin complexISOSGD:S000004521GO_REF:00000242
GO:0032153cell division siteIDAPMID:23956092298
IDAPMID:16823372
GO:0005737cytoplasm4204
during interphaseIDAPMID:23956092
GO:0005829cytosolIDAPMID:168233722319
IMPPMID:21811607
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000046decreased cell population growthCell growth assayppb1ΔNullPECO:0000137, PECO:0000038PMID:15657058382
FYPO:0000961normal growth on sorbitolCell growth assayppb1ΔNullPECO:0000005, PECO:0000102PMID:2181160713
FYPO:0001470normal growth on tacrolimusCell growth assayppb1ΔNullPECO:0000005, PECO:0000102PMID:218116075
FYPO:0001472normal growth on tacrolimus during osmotic stressCell growth assayppb1ΔNullPECO:0000005, PECO:0000025, PECO:0000102PMID:218116073
FYPO:0001471normal growth on tacrolimus during salt stressCell growth assayppb1ΔNullPECO:0000005, PECO:0000102PMID:218116071
FYPO:0001357normal vegetative cell population growthCell growth assayppb1ΔNullPMID:9427748618
Cell growth assayppb1ΔNullPECO:0000137PMID:15657058
FYPO:0001379resistance to tacrolimusSubstance quantificationppb1ΔNullPECO:0000092PMID:218116078
FYPO:0002641sensitive to micafunginCell growth assayppb1ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:17287531116
FYPO:0000271sensitive to salt stressCell growth assayppb1ΔNullPECO:0000031, PECO:0000005, PECO:0000102PMID:2181160782
Cell growth assayppb1ΔNullPECO:0000038, PECO:0000005, PECO:0000102PMID:21811607
Cell growth assayppb1ΔNullPECO:0000092, PECO:0000005, PECO:0000102PMID:21811607
Cell growth assayppb1ΔNullPECO:0000201, PECO:0000214, PECO:0000102PMID:10365961
Cell growth assayppb1ΔNullPECO:0000032PMID:9427748
FYPO:0000091sensitive to thiabendazolecompetitive growth assay evidenceppb1ΔNullPMID:20537132175
FYPO:0000115sensitive to valproic acidCell growth assayppb1ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:17287531155
FYPO:0002060viable vegetative cell populationMicroscopyppb1ΔNullPMID:204732893751
Microscopyppb1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000015branched vegetative cellMicroscopynot recorded (unrecorded)PMID:798314254
FYPO:0000134branched, elongated, multiseptate cellMicroscopyppb1ΔNullPMID:1565705810
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidenceppb1ΔNullPECO:0000015PMID:23950735238
FYPO:0002869decreased protein localization to cell division site3
affecting spn1Microscopyppb1ΔNullPMID:23956092
affecting spn3Microscopyppb1ΔNullPMID:23956092
affecting spn2Microscopyppb1ΔNullPMID:23956092
FYPO:0001198increased cellular calcium levelSubstance quantificationppb1ΔNullPMID:218116079
FYPO:0001378increased cellular calcium level during cellular response to salt stressSubstance quantificationppb1ΔNullPECO:0000092PMID:218116075
FYPO:0000118multiseptateMicroscopyppb1ΔNullPMID:9427748101
Microscopyppb1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001510viable vegetative cell, abnormal cell shape, normal cell size215
expressivity FYPO_EXT:0000003Microscopyppb1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in rho1 Rho family GTPase Rho1 PMID:23934882
GO regulated by cnb1 calcineurin regulatory subunit (calcineurin B) PMID:15657058
GO regulated by sep1 forkhead transcription factor Sep1 PMID:18057023
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
128738422874135
228741802874386
328745412876109
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00149 Pfam IPR004843 Phosphoesterase domain 113 313 20
SM00156 SMART IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 85 376 10
PS00125 Prosite Patterns IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 176 181 10
PTHR11668 HMMPANTHER 13 464 11
PTHR11668:SF118 HMMPANTHER 13 464 1
3.60.21.10 Gene3D 65 398 23
SSF56300 SuperFamily Metallo-dependent phosphatase-like 42 529 22
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 113 140 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 238 265 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 175 199 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 209 235 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 302 322 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 142 169 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 330 346 10

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0004243PPP3/PP2B/calcineurin subfamilyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0004243

Protein Properties

Ave. residue weight 115.01 Da
Charge -6.50
Isoelectric point 5.85
Molecular weight 63.71 kDa
Number of residues 554
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS534PMID:247631071670
S533PMID:24763107
S498PMID:24763107
present during mitotic M phaseS539PMID:21712547
present during mitotic M phaseS534PMID:21712547
present during mitotic M phaseS533PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0000087experimental evidencePMID:7634333

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8611during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7406during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
8621during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
9293during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3351.59during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
8795during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3163.19during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.4during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pmc1mediator complex subunit Pmc1 Dosage RescuePMID:23271606
sts1C-24(28) sterol reductase Sts1 Synthetic LethalityPMID:7983142
pmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:9427748
Synthetic Lethality
Dosage RescuePMID:17881729
Dosage RescuePMID:12931193
Dosage RescuePMID:10365961
Dosage RescuePMID:15657058
Dosage RescuePMID:12637524
ppe1serine/threonine protein phosphatase Ppe1 Synthetic Growth DefectPMID:7983142
imp2contractile ring protein Imp2 Synthetic LethalityPMID:19139265
cdc14SIN component Cdc14 Dosage RescuePMID:12354095
rho1Rho family GTPase Rho1 Synthetic LethalityPMID:23934882
Dosage Rescue
pek1MAP kinase kinase Pek1 Dosage RescuePMID:12931193
Synthetic RescuePMID:17005909
Synthetic Growth DefectPMID:10365961
Dosage Rescue
Synthetic Rescue
cdc7serine/threonine protein kinase Cdc7 Dosage RescuePMID:12354095
Synthetic LethalityPMID:12637524
cdc11SIN component scaffold protein, centriolin ortholog Cdc11 Dosage RescuePMID:12354095
gdi1GDP dissociation inhibitor Gdi1 (predicted) Synthetic LethalityPMID:16980382
ecm33cell wall protein Ecm33 Dosage RescuePMID:20032302
pmk1MAP kinase Pmk1 Synthetic RescuePMID:23738021
Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
Synthetic RescuePMID:10365961
tea2kinesin-like protein Tea2 Phenotypic SuppressionPMID:21336311
eso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
ptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
spg1GTPase Spg1 Dosage RescuePMID:12354095
cpp1protein farnesyltransferase beta subunit Cpp1 Synthetic RescuePMID:17005909
ptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Dosage RescuePMID:12354095
its8pig-N (predicted) Synthetic LethalityPMID:12637524
its31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:10950958
Synthetic LethalityPMID:12637524
cds1replication checkpoint kinase Cds1 Phenotypic SuppressionPMID:21336311
sts5RNB-like protein Synthetic LethalityPMID:7983142
Synthetic LethalityPMID:8886983
pck2protein kinase C (PKC)-like Pck2 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
ypt3GTPase Ypt3 Synthetic LethalityPMID:12181359
Synthetic LethalityPMID:12637524
cnb1calcineurin regulatory subunit (calcineurin B) Phenotypic EnhancementPMID:15657058
Dosage Growth Defect
apm1AP-1 adaptor complex mu subunit Apm1 Synthetic LethalityPMID:15047861
rnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Dosage RescuePMID:12931193
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
myp2myosin II heavy chain Myo3 Synthetic RescuePMID:12136004
Synthetic Lethality
sid2NDR kinase Sid2 Dosage RescuePMID:12354095
prz1calcineurin responsive transcription factor Prz1 Synthetic RescuePMID:15657058
mkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
Synthetic RescuePMID:10365961
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Synthetic LethalityPMID:12637524
tip1CLIP170 family protein Tip1 Phenotypic SuppressionPMID:21336311
ppa2serine/threonine protein phosphatase Ppa2 Synthetic Growth DefectPMID:7983142
kin1microtubule affinity-regulating kinase Kin1 Phenotypic EnhancementPMID:23294323
Dosage Rescue
Synthetic Lethality
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tip1CLIP170 family protein Tip1 Affinity Capture-WesternPMID:21336311
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
cds1replication checkpoint kinase Cds1 Affinity Capture-WesternPMID:21336311
tea2kinesin-like protein Tea2 Affinity Capture-WesternPMID:21336311
cnb1calcineurin regulatory subunit (calcineurin B) Affinity Capture-WesternPMID:15657058
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPBP4H10.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBP4H10.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBP4H10.04 BioGRID Interaction Datasets
Expression Viewer SPBP4H10.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBP4H10.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBP4H10.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBP4H10.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBP4H10.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBP4H10.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBP4H10.04 Cell Cycle Data
GEO SPBP4H10.04 GEO profiles
PInt SPBP4H10.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBP4H10.04 Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz3.1.3.16Integrated relational Enzyme database
Rhea3.1.3.16Annotated reactions database
SPD / RIKEN33/33E08Orfeome Localization Data
UniProtKB/SwissProtQ12705Serine/threonine-protein phosphatase 2B catalytic subunit
ModBaseQ12705Database of comparative protein structure models
STRINGQ12705Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596178calcium-dependent serine/threonine protein phosphatase calcineurin, catalytic subunit Ppb1
RefSeq mRNANM_001022097972h- calcium-dependent serine/threonine protein phosphatase calcineurin, catalytic subunit Ppb1 (ppb1), mRNA
European Nucleotide ArchiveBAA06081.1ENA Protein Mapping
European Nucleotide ArchiveCAB83162.1ENA Protein Mapping
UniParcUPI0000130FD2UniProt Archive

Literature for ppb1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014