ppb1 (SPBP4H10.04)


Gene Standard Nameppb1 Characterisation Statuspublished
Systematic IDSPBP4H10.04 Feature Typeprotein coding
SynonymsSPBC1346.01c Name Description
Productcalcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Product Size554aa, 63.71 kDa
Genomic Location Chromosome II, 2873842-2876109 (2268nt); CDS:2873997-2875859 (1863nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calmodulin binding13
Annotation ExtensionEvidenceWith/FromReference
metal ion binding750
Annotation ExtensionEvidenceWith/FromReference
phosphoprotein phosphatase activity35
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular chloride ion homeostasis2
Annotation ExtensionEvidenceWith/FromReference
cellular response to salt stress14
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall organization or biogenesis99
Annotation ExtensionEvidenceWith/FromReference
negative regulation of calcium ion transport into cytosol1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
calcineurin complex2
Annotation ExtensionEvidenceWith/FromReference
cell division site313
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyppb1Δ272
decreased vegetative cell population growthppb1Δ652
ppb1::ura4+
normal growth on clotrimazolePpb1ΔC13
ppb1+ (wild type)
normal growth on cyclosporin Appb1::ura4+1
normal growth on glucose carbon sourceppb1Δ273
normal growth on micafunginPpb1ΔC5
ppb1+ (wild type)
normal growth on terbinafineppb1+ (wild type)41
normal vegetative cell population growthppb1::ura4+833
ppb1Δ
sensitive to BE49385Appb1Δ2
sensitive to calciumppb1Δ22
sensitive to micafunginppb1Δ119
sensitive to salt stressppb1Δ98
sensitive to tamoxifenPpb1ΔC58
ppb1+ (wild type)
sensitive to terbinafinePpb1ΔC103
sensitive to thiabendazoleppb1Δ264
sensitive to valproic acidppb1Δ155
viable vegetative cell populationppb1Δ3837

Cell Phenotype

Term NameGenotypesCount
abnormal nuclear morphology during vegetative growthppb1+ (wild type)75
abnormal spindle pole body localizationppb1+ (wild type)3
branched, elongated cell with branch forming adjacent to septumppb1::ura4+1
branched, elongated, multiseptate cellppb1Δ13
decreased protein localization to cell division site27
affects localization of spn1ppb1Δ
affects localization of spn2ppb1Δ
affects localization of spn3ppb1Δ
decreased rate of cytokinesisppb1::ura4+10
elongated multiseptate vegetative cell, with three or more nuclei and single septa between nucleippb1::ura4+17
elongated vegetative cellppb1::ura4+791
increased cytosolic calcium levelppb1Δ6
multiseptate vegetative cellppb1Δ97
sterileppb1::ura4+61
viable curved elongated vegetative cellppb1+ (wild type)20
viable vegetative cell, abnormal cell shape, normal cell sizeppb1Δ215

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
slow vegetative cell population growthppb1Δ, prz1+ (wild type)
viable vegetative cell populationppb1Δ, par2Δ

Cell Phenotype

Term NameGenotypes
increased transcription during cellular response to calcium ion
affecting SO:0001865ppb1Δ, prz1+ (wild type)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in rho1 Rho family GTPase Rho1
GO regulated by cnb1 calcineurin regulatory subunit (calcineurin B)
GO regulated by sep1 forkhead transcription factor Sep1
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2873842..2874135, 2874180..2874386, 2874541..2876109
Intron2874136..2874179, 2874387..2874540
5' UTR2873842..2873996PMID:21511999
3' UTR2875860..2876109PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00149 Pfam IPR004843 Calcineurin-like phosphoesterase domain, apaH type 113 313 20
SM00156 SMART IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 85 376 10
PS00125 Prosite Patterns IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 176 181 10
PTHR11668 HMMPANTHER 427 491 11
PTHR11668 HMMPANTHER 12 410 11
PTHR11668:SF118 HMMPANTHER 427 491 1
PTHR11668:SF118 HMMPANTHER 12 410 1
3.60.21.10 Gene3D IPR029052 Metallo-dependent phosphatase-like 65 398 23
SSF56300 SuperFamily IPR029052 Metallo-dependent phosphatase-like 42 529 22
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 175 199 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 142 169 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 238 265 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 209 235 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 330 346 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 302 322 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 113 140 10

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0004243PPP3/PP2B/calcineurin subfamilyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0004243

Protein Properties

Ave. residue weight 115.01 Da
Charge -6.50
Codon Adaptation Index 0.45
Isoelectric point 5.85
Molecular weight 63.71 kDa
Number of residues 554
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS539
present during mitotic M phaseS534
present during mitotic M phaseS533
S498, S533, S534
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S539 PMID:21712547
present during mitotic M phase experimental evidence S534 PMID:21712547
present during mitotic M phase experimental evidence S533 PMID:21712547
experimental evidence S534 PMID:24763107
experimental evidence S533 PMID:24763107
experimental evidence S498 PMID:24763107
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0010458Northern assay evidencePMID:12411492
increased during GO:0000087experimental evidencePMID:7634333

Quantitative Gene Expression

View graphical display of gene expression data for ppb1 (SPBP4H10.04)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8611during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
7406during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8621during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9293during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3351.59during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
8795during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3163.19during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.4during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
promoter contains PCB PMID:124114928
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPBP4H10.04 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bytip1CLIP170 family protein Tip1 Affinity Capture-WesternPMID:21336311
affinity captured bycds1replication checkpoint kinase Cds1 Affinity Capture-WesternPMID:21336311
affinity captured bycnb1calcineurin regulatory subunit (calcineurin B) Affinity Capture-WesternPMID:15657058
affinity captured bytea2kinesin-like protein Tea2 Affinity Capture-WesternPMID:21336311
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPBP4H10.04 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetically rescued bymkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:17005909
synthetically rescued bypmk1MAP kinase Pmk1 Synthetic RescuePMID:10365961
synthetically rescued bypck2protein kinase C (PKC)-like Pck2 Synthetic RescuePMID:17005909
synthetically rescued byprz1calcineurin responsive transcription factor Prz1 Synthetic RescuePMID:15657058
synthetically rescued bymyp2myosin II heavy chain Myo3 Synthetic RescuePMID:12136004
synthetically rescued bycpp1protein farnesyltransferase beta subunit Cpp1 Synthetic RescuePMID:17005909
synthetically rescued bypek1MAP kinase kinase Pek1 Synthetic RescuePMID:17005909
synthetic lethal withcdc7serine/threonine protein kinase Cdc7 Synthetic LethalityPMID:12637524
synthetic lethal withits31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:10950958
synthetic lethal withbgs11,3-beta-glucan synthase catalytic subunit Bgs1 Synthetic LethalityPMID:12637524
synthetic lethal withpmp1dual-specificity MAP kinase phosphatase Pmp1 Synthetic LethalityPMID:9427748
synthetic lethal witherg4C-24(28) sterol reductase Erg4 Synthetic LethalityPMID:7983142
synthetic lethal withapm1AP-1 adaptor complex mu subunit Apm1 Synthetic LethalityPMID:15047861
synthetic lethal withimp2contractile ring protein Imp2 Synthetic LethalityPMID:19139265
synthetic lethal withypt3GTPase Ypt3 Synthetic LethalityPMID:12181359
synthetic lethal withits8pig-N (predicted) Synthetic LethalityPMID:12637524
synthetic lethal withsts5RNB-like protein Synthetic LethalityPMID:7983142
synthetic lethal withmyp2myosin II heavy chain Myo3 Synthetic LethalityPMID:12136004
synthetic lethal withkin1microtubule affinity-regulating kinase Kin1 Synthetic LethalityPMID:23294323
synthetic lethal withgdi1GDP dissociation inhibitor Gdi1 (predicted) Synthetic LethalityPMID:16980382
synthetic lethal withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
synthetic lethal withrho1Rho family GTPase Rho1 Synthetic LethalityPMID:23934882
synthetic lethal withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Synthetic LethalityPMID:11380623
overexpression rescuescdc7serine/threonine protein kinase Cdc7 Dosage RescuePMID:12354095
overexpression rescuessid2NDR kinase Sid2 Dosage RescuePMID:12354095
overexpression rescuescdc14SIN component Cdc14 Dosage RescuePMID:12354095
overexpression rescuesspg1GTPase Spg1 Dosage RescuePMID:12354095
overexpression rescuesmed14mediator complex subunit Med14 Dosage RescuePMID:23271606
overexpression rescuespmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:9427748
overexpression rescuescdc11SIN component scaffold protein, centriolin ortholog Cdc11 Dosage RescuePMID:12354095
overexpression rescuesmob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Dosage RescuePMID:12354095
overexpression rescueskin1microtubule affinity-regulating kinase Kin1 Dosage RescuePMID:23294323
negative genetic interaction witheso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
rescued by overexpression ofpmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:12931193
rescued by overexpression ofrnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Dosage RescuePMID:12931193
rescued by overexpression ofptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
rescued by overexpression ofptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
rescued by overexpression ofpek1MAP kinase kinase Pek1 Dosage RescuePMID:12931193
rescued by overexpression ofecm33cell wall protein Ecm33 Dosage RescuePMID:20032302
rescued by overexpression oftrp1322TRP-like ion channel (predicted) Dosage RescuePMID:21811607
synthetic growth defect withppa2serine/threonine protein phosphatase Ppa2 Synthetic Growth DefectPMID:7983142
synthetic growth defect withppe1serine/threonine protein phosphatase Ppe1 Synthetic Growth DefectPMID:7983142
synthetic growth defect withpek1MAP kinase kinase Pek1 Synthetic Growth DefectPMID:10365961
rescued bytip1CLIP170 family protein Tip1 Phenotypic SuppressionPMID:21336311
rescued bytea2kinesin-like protein Tea2 Phenotypic SuppressionPMID:21336311
rescued bycds1replication checkpoint kinase Cds1 Phenotypic SuppressionPMID:21336311
enhances phenotype ofcnb1calcineurin regulatory subunit (calcineurin B) Phenotypic EnhancementPMID:15657058
enhances phenotype ofkin1microtubule affinity-regulating kinase Kin1 Phenotypic EnhancementPMID:23294323
overexpression causes growth defect tocnb1calcineurin regulatory subunit (calcineurin B) Dosage Growth DefectPMID:15657058
synthetically rescuespmk1MAP kinase Pmk1 Synthetic RescuePMID:23738021
synthetically rescuesrho1Rho family GTPase Rho1 Synthetic RescuePMID:23934882
rescuescmk1calcium/calmodulin-dependent protein kinase Cmk1 Phenotypic SuppressionPMID:25081204
External References
Database Identifier Description
NBRP SPBP4H10.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBP4H10.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBP4H10.04 BioGRID Interaction Datasets
Expression Viewer SPBP4H10.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBP4H10.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBP4H10.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBP4H10.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBP4H10.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBP4H10.04 Transcriptome Viewer (Bähler Lab)
GEO SPBP4H10.04 GEO profiles
PInt SPBP4H10.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBP4H10.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBP4H10.04 Fission yeast phenotypic data & analysis
Cyclebase SPBP4H10.04.1 Cell Cycle Data
SPD / RIKEN33/33E08Orfeome Localization Data
IntEnz3.1.3.16Integrated relational Enzyme database
Rhea3.1.3.16Annotated reactions database
UniProtKB/SwissProtQ12705Serine/threonine-protein phosphatase 2B catalytic subunit
ModBaseQ12705Database of comparative protein structure models
STRINGQ12705Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596178calcium-dependent serine/threonine protein phosphatase calcineurin, catalytic subunit Ppb1
RefSeq mRNANM_001022097972h- calcium-dependent serine/threonine protein phosphatase calcineurin, catalytic subunit Ppb1 (ppb1), mRNA

Literature for ppb1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016