ppb1 (SPBP4H10.04)


Gene Standard Nameppb1 Characterisation Statuspublished
Systematic IDSPBP4H10.04 Feature Typeprotein coding
SynonymsSPBC1346.01c Name Description
Productcalcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Product Size554aa, 63.71 kDa
Genomic Location Chromosome II, 2873842-2876109 (2268nt); CDS:2873997-2875859 (1863nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium-dependent protein serine/threonine phosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
calmodulin binding10
Annotation ExtensionEvidenceWith/FromReference
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
phosphatase activity70
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
calcium-mediated signaling12
Annotation ExtensionEvidenceWith/FromReference
cellular chloride ion homeostasis2
Annotation ExtensionEvidenceWith/FromReference
cellular response to salt stress17
Annotation ExtensionEvidenceWith/FromReference
cytosolic calcium ion homeostasis7
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall organization or biogenesis120
Annotation ExtensionEvidenceWith/FromReference
negative regulation of calcium ion transport into cytosol2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
calcineurin complex2
Annotation ExtensionEvidenceWith/FromReference
cell division site299
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4206
Annotation ExtensionEvidenceWith/FromReference
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growthppb1ΔNull431
decreased mating efficiencyppb1ΔNull247
decreased vegetative cell population growthppb1::ura4+ (disruption)Null400
normal growth on cyclosporin Appb1::ura4+ (disruption)Null1
normal growth on glucose carbon sourceppb1ΔNull189
normal growth on sorbitolppb1ΔNull17
normal growth on tacrolimusppb1ΔNull5
normal growth on tacrolimus during osmotic stressppb1ΔNull3
normal growth on tacrolimus during salt stressppb1ΔNull1
normal vegetative cell population growthppb1::ura4+ (disruption)Null685
ppb1ΔNull
resistance to tacrolimusppb1ΔNull8
sensitive to micafunginppb1ΔNull116
sensitive to salt stressppb1ΔNull86
sensitive to thiabendazoleppb1ΔNull185
sensitive to valproic acidppb1ΔNull155
viable vegetative cell populationppb1ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
abnormal nuclear morphology during vegetative growthppb1+ (wild type)Overexpression55
abnormal spindle pole body localizationppb1+ (wild type)Overexpression2
branched, elongated cell with branch forming adjacent to septum1
penetrance FYPO_EXT:0000003, expressivity FYPO_EXT:0000002ppb1::ura4+ (disruption)Null
branched, elongated, multiseptate cellppb1ΔNull11
decreased protein localization to cell division site16
affecting spn1ppb1ΔNull
affecting spn2ppb1ΔNull
affecting spn3ppb1ΔNull
decreased rate of cytokinesisppb1::ura4+ (disruption)Null12
elongated multinucleate multiseptate vegetative cell, septa between nuclei15
penetrance FYPO_EXT:0000002ppb1::ura4+ (disruption)Null
penetrance FYPO_EXT:0000003ppb1::ura4+ (disruption)Null
elongated vegetative cell703
penetrance FYPO_EXT:0000001, expressivity FYPO_EXT:0000002ppb1::ura4+ (disruption)Null
increased cellular calcium levelppb1ΔNull9
increased cellular calcium level during cellular response to salt stressppb1ΔNull5
multiseptateppb1ΔNull114
sterileppb1::ura4+ (disruption)Null54
viable curved elongated vegetative cell15
expressivity FYPO_EXT:0000003ppb1+ (wild type)Overexpression
viable vegetative cell, abnormal cell shape, normal cell size215
expressivity FYPO_EXT:0000003ppb1ΔNull
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in rho1 Rho family GTPase Rho1 PMID:23934882
GO regulated by cnb1 calcineurin regulatory subunit (calcineurin B) PMID:15657058
GO regulated by sep1 forkhead transcription factor Sep1 PMID:18057023
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
128738422874135
228741802874386
328745412876109

UTRs

Region Coordinates Reference
five_prime_UTR2873842..2873996PMID:21511999
three_prime_UTR2875860..2876109PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00149 Pfam IPR004843 Calcineurin-like phosphoesterase domain, apaH type 113 313 20
SM00156 SMART IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 85 376 10
PS00125 Prosite Patterns IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 176 181 10
PTHR11668 HMMPANTHER 39 545 11
PTHR11668:SF118 HMMPANTHER 39 545 1
3.60.21.10 Gene3D IPR029052 Metallo-dependent phosphatase-like 65 398 23
SSF56300 SuperFamily IPR029052 Metallo-dependent phosphatase-like 42 529 22
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 142 169 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 302 322 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 175 199 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 238 265 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 330 346 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 113 140 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 209 235 10

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0004243PPP3/PP2B/calcineurin subfamilyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0004243

Protein Properties

Ave. residue weight 115.01 Da
Charge -6.50
Isoelectric point 5.85
Molecular weight 63.71 kDa
Number of residues 554
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS539
present during mitotic M phaseS533
present during mitotic M phaseS534
S498, S533, S534
Annotation ExtensionEvidenceResidueReference
experimental evidence S498 PMID:24763107
experimental evidence S533 PMID:24763107
present during mitotic M phase experimental evidence S533 PMID:21712547
experimental evidence S534 PMID:24763107
present during mitotic M phase experimental evidence S534 PMID:21712547
present during mitotic M phase experimental evidence S539 PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0000087experimental evidencePMID:7634333

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8611during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7406during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
8621during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
9293during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3351.59during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
8795during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3163.19during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.4during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
tip1CLIP170 family protein Tip1 Affinity Capture-WesternPMID:21336311
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
cds1replication checkpoint kinase Cds1 Affinity Capture-WesternPMID:21336311
tea2kinesin-like protein Tea2 Affinity Capture-WesternPMID:21336311
cnb1calcineurin regulatory subunit (calcineurin B) Affinity Capture-WesternPMID:15657058
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
pmc1mediator complex subunit Pmc1 Dosage RescuePMID:23271606
cmk1calcium/calmodulin-dependent protein kinase Cmk1 Phenotypic SuppressionPMID:25081204
sts1C-24(28) sterol reductase Sts1 Synthetic LethalityPMID:7983142
pmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:9427748
Synthetic Lethality
Dosage RescuePMID:17881729
Dosage RescuePMID:12931193
Dosage RescuePMID:10365961
Dosage RescuePMID:15657058
Dosage RescuePMID:12637524
ppe1serine/threonine protein phosphatase Ppe1 Synthetic Growth DefectPMID:7983142
imp2contractile ring protein Imp2 Synthetic LethalityPMID:19139265
cdc14SIN component Cdc14 Dosage RescuePMID:12354095
rho1Rho family GTPase Rho1 Dosage RescuePMID:23934882
Synthetic Lethality
pek1MAP kinase kinase Pek1 Dosage RescuePMID:12931193
Synthetic RescuePMID:17005909
Dosage RescuePMID:10365961
Synthetic Growth Defect
Synthetic Rescue
cdc7serine/threonine protein kinase Cdc7 Dosage RescuePMID:12354095
Synthetic LethalityPMID:12637524
cdc11SIN component scaffold protein, centriolin ortholog Cdc11 Dosage RescuePMID:12354095
gdi1GDP dissociation inhibitor Gdi1 (predicted) Synthetic LethalityPMID:16980382
ecm33cell wall protein Ecm33 Dosage RescuePMID:20032302
pmk1MAP kinase Pmk1 Synthetic RescuePMID:23738021
Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
Synthetic RescuePMID:10365961
tea2kinesin-like protein Tea2 Phenotypic SuppressionPMID:21336311
eso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
ptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
spg1GTPase Spg1 Dosage RescuePMID:12354095
cpp1protein farnesyltransferase beta subunit Cpp1 Synthetic RescuePMID:17005909
ptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Dosage RescuePMID:12354095
its8pig-N (predicted) Synthetic LethalityPMID:12637524
its31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:10950958
Synthetic LethalityPMID:12637524
cds1replication checkpoint kinase Cds1 Phenotypic SuppressionPMID:21336311
sts5RNB-like protein Synthetic LethalityPMID:7983142
Synthetic LethalityPMID:8886983
pck2protein kinase C (PKC)-like Pck2 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
ypt3GTPase Ypt3 Synthetic LethalityPMID:12181359
Synthetic LethalityPMID:12637524
cnb1calcineurin regulatory subunit (calcineurin B) Phenotypic EnhancementPMID:15657058
Dosage Growth Defect
apm1AP-1 adaptor complex mu subunit Apm1 Synthetic LethalityPMID:15047861
rnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Dosage RescuePMID:12931193
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
myp2myosin II heavy chain Myo3 Synthetic LethalityPMID:12136004
Synthetic Rescue
sid2NDR kinase Sid2 Dosage RescuePMID:12354095
prz1calcineurin responsive transcription factor Prz1 Synthetic RescuePMID:15657058
mkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
Synthetic RescuePMID:10365961
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Synthetic LethalityPMID:12637524
tip1CLIP170 family protein Tip1 Phenotypic SuppressionPMID:21336311
ppa2serine/threonine protein phosphatase Ppa2 Synthetic Growth DefectPMID:7983142
kin1microtubule affinity-regulating kinase Kin1 Dosage RescuePMID:23294323
Synthetic Lethality
Phenotypic Enhancement
External References
Database Identifier Description
NBRP SPBP4H10.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBP4H10.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBP4H10.04 BioGRID Interaction Datasets
Expression Viewer SPBP4H10.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBP4H10.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBP4H10.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBP4H10.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBP4H10.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPBP4H10.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPBP4H10.04 Cell Cycle Data
GEO SPBP4H10.04 GEO profiles
PInt SPBP4H10.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBP4H10.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBP4H10.04 Fission yeast phenotypic data & analysis
IntEnz3.1.3.16Integrated relational Enzyme database
Rhea3.1.3.16Annotated reactions database
SPD / RIKEN33/33E08Orfeome Localization Data
UniProtKB/SwissProtQ12705Serine/threonine-protein phosphatase 2B catalytic subunit
ModBaseQ12705Database of comparative protein structure models
STRINGQ12705Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596178calcium-dependent serine/threonine protein phosphatase calcineurin, catalytic subunit Ppb1
RefSeq mRNANM_001022097972h- calcium-dependent serine/threonine protein phosphatase calcineurin, catalytic subunit Ppb1 (ppb1), mRNA
European Nucleotide ArchiveBAA06081.1ENA Protein Mapping
European Nucleotide ArchiveCAB83162.1ENA Protein Mapping
UniParcUPI0000130FD2UniProt Archive

Literature for ppb1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015