ppb1 (SPBP4H10.04)

Gene Standard Nameppb1 ChromosomeII
Systematic IDSPBP4H10.04 Gene Start2873842
SynonymsSPBC1346.01c Gene End2876109
Productcalcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Gene Length2268
Feature Typeprotein coding CDS Start2873997
Name Description CDS End2875859
Characterisation Statuspublished CDS Length1863

Ensembl Gene Location
  1. lid2
  2. SPNCRNA.1557
  3. SPBP19A11.07c
  4. oxa102
  5. ppb1
  6. spe2
  7. cut14
  8. SPBP4H10.07
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000091sensitive to thiabendazolecompetitive growth assay evidenceppb1delta (deletion)deletionPMID:20537132
FYPO:0000118multiseptateMicroscopyppb1delta (deletion)deletionPMID:9427748
FYPO:0000269sensitive to microtubule depolymerizing drugscompetitive growth assay evidenceppb1delta (deletion)deletionPMID:20537132
FYPO:0000271sensitive to salt stressCell growth assayppb1delta (deletion)deletionPECO:0000032PMID:9427748
Cell growth assayppb1delta (deletion)deletionPECO:0000038, PECO:0000005, PECO:0000102PMID:21811607
Cell growth assayppb1delta (deletion)deletionPECO:0000092, PECO:0000005, PECO:0000102PMID:21811607
Cell growth assayppb1delta (deletion)deletionPECO:0000031, PECO:0000005, PECO:0000102PMID:21811607
FYPO:0001198increased cellular calcium levelSubstance quantificationppb1delta (deletion)deletionPMID:21811607
FYPO:0001378increased cellular calcium level during cellular response to salt stressSubstance quantificationppb1delta (deletion)deletionPECO:0000092PMID:21811607
FYPO:0001379resistance to tacrolimusSubstance quantificationppb1delta (deletion)deletionPECO:0000092PMID:21811607
FYPO:0002060viable vegetative cell populationMicroscopyppb1delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0004723calcium-dependent protein serine/threonine phosphatase activityIMPPMID:16928959
GO:0005516calmodulin bindingIEAUniProtKB:KW-0112GO_REF:0000037
GO:0016791phosphatase activity
requires_regulator cnb1IDAPMID:15657058
has_direct_input prz1IDAPMID:15657058
GO:0046872metal ion bindingIEAUniProtKB:KW-0479GO_REF:0000037
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0006470protein dephosphorylationICGO:0004721GO_REF:0000001
GO:0010523negative regulation of calcium ion transport into cytosolIMPPMID:21811607
GO:0019722calcium-mediated signalingIMPPMID:16928959
GO:0030644cellular chloride ion homeostasisIMPPMID:9427748
GO:0031137regulation of conjugation with cellular fusionIMPPMID:7983142
GO:0032465regulation of cytokinesisIGIits8PMID:10950958
requiredIMPPMID:7983142
GO:0051480cytosolic calcium ion homeostasisIMPPMID:21811607
GO:0070631spindle pole body localizationIMPPMID:1668885
GO:0071472cellular response to salt stressIMPPMID:21811607
IMPPMID:9427748
GO:0071852fungal-type cell wall organization or biogenesisIGIits8PMID:1095095
GO:2000769regulation of establishment or maintenance of cell polarity regulating cell shapeIMPPMID:7983142
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005829cytosolIDAPMID:16823372
GO:0005955calcineurin complexISOSGD:S000004521GO_REF:0000024
GO:0032153cell division siteIDAPMID:16823372
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
128738422874135
228741802874386
328745412876109

References

Region Start End Reference
three_prime_UTR28758602876109PMID:21511999
five_prime_UTR28738422873996PMID:21511999
three_prime_UTR28758602876109PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00149 IPR004843 Pfam Metallophosphoesterase domain 113 313 20
SM00156 IPR006186 SMART Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 85 376 10
PS00125 IPR006186 Prosite Patterns Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 176 181 10
G3DSA:3.60.21.10 Gene3D 65 398 22
SSF56300 SuperFamily 42 529 22
PR00114 IPR006186 PRINTS Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 209 235 10
PR00114 IPR006186 PRINTS Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 142 169 10
PR00114 IPR006186 PRINTS Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 330 346 10
PR00114 IPR006186 PRINTS Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 302 322 10
PR00114 IPR006186 PRINTS Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 175 199 10
PR00114 IPR006186 PRINTS Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 238 265 10
PR00114 IPR006186 PRINTS Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 113 140 10
PTHR11668 hmmpanther 7 515 10
PTHR11668:SF15 hmmpanther 7 515 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0004243PPP3/PP2B/calcineurin subfamilyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0004243

Protein Properties

Ave. residue weight 115.01 Da
Charge -6.50
Isoelectric point 5.85
Molecular weight 63.71 kDa
Number of residues 554
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
expressed during M-G1 phase PMID:7634333
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide3163.19PMID:23101633
experimental evidencePECO:0000014,
PECO:0000005		population_wide3351.59PMID:23101633
RNA levelexperimental evidencePECO:0000014,
PECO:0000005		population_wide2.4PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.8PMID:23101633
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
tea2kinesin-like protein Tea2 Phenotypic SuppressionPMID:21336311
prz1calcineurin responsive transcription factor Prz1 Synthetic RescuePMID:15657058
sts1C-24(28) sterol reductase Sts1 Synthetic LethalityPMID:7983142
imp2contractile ring protein Imp2 Synthetic LethalityPMID:19139265
spg1GTPase Spg1 Dosage RescuePMID:12354095
apm1AP-1 adaptor complex mu subunit Apm1 Synthetic LethalityPMID:15047861
sid2NDR kinase Sid2 Dosage RescuePMID:12354095
tip1CLIP170 family protein Tip1 Phenotypic SuppressionPMID:21336311
cnb1calcineurin regulatory subunit (calcineurin B) Phenotypic EnhancementPMID:15657058
Dosage Growth Defect
ppe1serine/threonine protein phosphatase Ppe1 Synthetic Growth DefectPMID:7983142
pek1MAP kinase kinase Pek1 Synthetic RescuePMID:17005909
Dosage RescuePMID:12931193
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Dosage RescuePMID:12354095
sts5RNB-like protein Synthetic LethalityPMID:7983142
Synthetic LethalityPMID:8886983
pmk1MAP kinase Pmk1 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
Synthetic RescuePMID:10365961
gdi1GDP dissociation inhibitor Gdi1 (predicted) Synthetic LethalityPMID:16980382
pck2protein kinase C (PKC)-like Pck2 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
cdc11SIN component scaffold protein, centriolin ortholog Cdc11 Dosage RescuePMID:12354095
ptc3protein phosphatase 2c homolog 3 Dosage RescuePMID:17881729
cdc7serine/threonine protein kinase Cdc7 Synthetic LethalityPMID:12637524
Dosage RescuePMID:12354095
ptc1protein phosphatase 2C Ptc1 Dosage RescuePMID:17881729
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
pmc1mediator complex subunit Pmc1 Dosage RescuePMID:23271606
ecm33cell wall protein Ecm33 Dosage RescuePMID:20032302
eso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
ppa2serine/threonine protein phosphatase Ppa2 Synthetic Growth DefectPMID:7983142
ypt3GTPase Ypt3 Synthetic LethalityPMID:12181359
Synthetic LethalityPMID:12637524
cds1replication checkpoint kinase Cds1 Phenotypic SuppressionPMID:21336311
pmp1dual-specificity MAP kinase phosphatase Pmp1 Dosage RescuePMID:9427748
Synthetic Lethality
Dosage RescuePMID:17881729
Dosage RescuePMID:12931193
Dosage RescuePMID:10365961
Dosage RescuePMID:12637524
Dosage RescuePMID:15657058
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Synthetic LethalityPMID:12637524
cdc14SIN component Cdc14 Dosage RescuePMID:12354095
myp2myosin II heavy chain Myo3 Synthetic RescuePMID:12136004
Synthetic Lethality
mkh1MEK kinase (MEKK) Mkh1 Synthetic RescuePMID:9427748
Synthetic RescuePMID:17005909
Synthetic RescuePMID:10365961
rnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Dosage RescuePMID:12931193
cpp1protein farnesyltransferase beta subunit Cpp1 Synthetic RescuePMID:17005909
its8pig-N (predicted) Synthetic LethalityPMID:12637524
kin1microtubule affinity-regulating kinase Kin1 Phenotypic EnhancementPMID:23294323
Dosage Rescue
Synthetic Lethality
its31-phosphatidylinositol-4-phosphate 5-kinase Its3 Synthetic LethalityPMID:12637524
Synthetic LethalityPMID:10950958
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tea2kinesin-like protein Tea2 Affinity Capture-WesternPMID:21336311
cds1replication checkpoint kinase Cds1 Affinity Capture-WesternPMID:21336311
tip1CLIP170 family protein Tip1 Affinity Capture-WesternPMID:21336311
cnb1calcineurin regulatory subunit (calcineurin B) Affinity Capture-WesternPMID:15657058
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External References
Database Identifier Description
NBRP SPBP4H10.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBP4H10.04 Retrieval of eukaryotic orthologs
BioGrid SPBP4H10.04 BioGRID Interaction Datasets
Bähler Lab SPBP4H10.04 Cell Cycle Expression Profile
Bähler Lab SPBP4H10.04 Meiosis/Sporulation Expression Profies
Bähler Lab SPBP4H10.04 Pheromone response/mating expression profiles
Bähler Lab SPBP4H10.04 Environmental stress expression profiles
Bähler Lab SPBP4H10.04 Bähler Lab Transcriptome Viewer
Cyclebase SPBP4H10.04 Cell Cycle Data
PInt SPBP4H10.04 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPBP4H10.04 Entrez Gene
IntEnz3.1.3.16
EntrezGene2540024serine/threonine protein phosphatase, calcineurin catalytic subunit Ppb1
Uniprot_genenamePPB1
WikiGene2540024serine/threonine protein phosphatase, calcineurin catalytic subunit Ppb1
UniProtKB/SwissProtQ12705Serine/threonine-protein phosphatase 2B catalytic subunit
ModBaseQ12705Database of comparative protein structure models
Pfam Protein DomainsQ12705Pfam Domain Arrangement
RefSeq PeptideNP_596178serine/threonine protein phosphatase, calcineurin catalytic subunit Ppb1
RefSeq mRNANM_001022097972h- serine/threonine protein phosphatase, calcineurin catalytic subunit Ppb1 (ppb1), mRNA
European Nucleotide ArchiveBAA06081ENA Protein Mapping
European Nucleotide ArchiveCAB83162ENA Protein Mapping
SPD / RIKEN33/33E08Orfeome Localization Data
UniParcUPI0000130FD2UniProt Archive

Literature for ppb1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013