lcf2 (SPBP4H10.11c)

Gene Standard Namelcf2 Characterisation Statuspublished
Systematic IDSPBP4H10.11c Feature Typeprotein coding
Synonyms Name Description
Productlong-chain-fatty-acid-CoA ligase Product Size689aa, 75.79 kDa
Genomic Location Chromosome II, 2894902-2892129 (2774nt); CDS:2894408-2892339 (2070nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
myristoyl-CoA ligase activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
long-chain fatty acid metabolic process5
Annotation ExtensionEvidenceWith/FromReference
N-terminal protein myristoylation2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
fungal-type vacuole membrane91
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
decreased cell density in stationary phaselcf2Δ4
increased viability in stationary phaselcf2Δ57
normal viability in stationary phaselcf2Δ24
viable vegetative cell populationlcf2Δ3815

Cell Phenotype

Term NameGenotypesCount
abnormal ascospore wall biogenesisSPBP4H10.11CΔ16
decreased myristoyl-CoA ligase activitylcf2Δ2
normal conjugation frequencySPBP4H10.11CΔ12
normal oleoyl-CoA ligase activitylcf2Δ1
normal palmitoyl-CoA ligase activitylcf2Δ1
spores sensitive to acetonelcf2Δ3
viable stubby vegetative celllcf2Δ72
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR2894902..2894409PMID:21511999
3' UTR2892338..2892129PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00501 Pfam IPR000873 AMP-dependent synthetase/ligase 93 546 9
PS00455 Prosite Patterns IPR020845 AMP-binding, conserved site 250 261 7
PTHR24096 HMMPANTHER 15 688 2
PTHR24096:SF153 HMMPANTHER 15 688 2 Gene3D AMP-dependent synthetase/ligase 462 534 7 Gene3D AMP-dependent synthetase/ligase 257 356 9 Gene3D AMP-dependent synthetase/ligase 399 455 9 Gene3D AMP-dependent synthetase/ligase 99 256 9 Gene3D AMP-dependent synthetase/ligase 42 64 9
SSF56801 SuperFamily 93 361 9
SSF56801 SuperFamily 626 686 9
SSF56801 SuperFamily 17 64 9
SSF56801 SuperFamily 402 597 9

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.00 Da
Charge 3.00
Codon Adpatation Index 0.53
Isoelectric point 6.91
Molecular weight 75.79 kDa
Number of residues 689
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
64886during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
67605during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
65680during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
63208during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
65269during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
16850.92during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
9396.81during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.2during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
1.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Disease Association
substrate myristic acid preferring PMID:180712491
Taxonomic Conservation
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3421
conserved in vertebrates3396
conserved in eukaryotes only2503

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
binds DNA-binding domain construct withskp1SCF ubiquitin ligase complex subunit Skp1 Two-hybridPMID:17016471
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
phenotype enhanced bylcf1long-chain-fatty-acid-CoA ligase Lcf1 Phenotypic EnhancementPMID:18071249
synthetic growth defect withlcf1long-chain-fatty-acid-CoA ligase Lcf1 Synthetic Growth DefectPMID:18071249
External References
Database Identifier Description
NBRP SPBP4H10.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBP4H10.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBP4H10.11c BioGRID Interaction Datasets
Expression Viewer SPBP4H10.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBP4H10.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBP4H10.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBP4H10.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBP4H10.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBP4H10.11c Transcriptome Viewer (Bähler Lab)
GEO SPBP4H10.11c GEO profiles
PInt SPBP4H10.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPBP4H10.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPBP4H10.11c Fission yeast phenotypic data & analysis
Cyclebase SPBP4H10.11c.1 Cell Cycle Data
SPD / RIKEN46/46E01Orfeome Localization Data
IntEnz6.2.1.3Integrated relational Enzyme database
Rhea6.2.1.3Annotated reactions database
UniProtKB/SwissProtQ9P7D7Long-chain-fatty-acid--CoA ligase 2
ModBaseQ9P7D7Database of comparative protein structure models
STRINGQ9P7D7Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596185long-chain-fatty-acid-CoA ligase
RefSeq mRNANM_001022104972h- long-chain-fatty-acid-CoA ligase (lcf2), mRNA
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveCAB83169ENA Protein Mapping
European Nucleotide ArchiveCAB83169.1ENA Protein Mapping
UniParcUPI000006AE1AUniProt Archive

Literature for lcf2

Search: Europe PMC or PubMed

Release Version: PomBase:28_55 - 09 Sep 2015