gal10 (SPBPB2B2.12c)


Gene Standard Namegal10 Characterisation Statuspublished
Systematic IDSPBPB2B2.12c Feature Typeprotein coding
Synonyms Name Description
ProductUDP-glucose 4-epimerase/aldose 1-epimerase Gal10 Product Size713aa, 80.67 kDa
Genomic Location Chromosome II, 4487569-4484023 (3547nt); CDS:4487418-4485277 (2142nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003978UDP-glucose 4-epimerase activity2
minorIDAPMID:19942659
GO:0004034aldose 1-epimerase activityIEA EC:5.1.3.3GO_REF:00000031
GO:0030246carbohydrate bindingIEAIPR011013GO_REF:000000215
GO:0050662coenzyme bindingIEAIPR001509GO_REF:000000291
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0033499galactose catabolic process via UDP-galactoseISSUniProtKB:P04397GO_REF:00000014
GO:0006012galactose metabolic processIMPPMID:210750507
GO:0042125protein galactosylationIMPPMID:1994265911
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopygal10ΔNullPECO:0000005, PECO:0000137PMID:236978063760
Microscopygal10ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopygal10ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
144875694484023

UTRs

Region Coordinates Reference
five_prime_UTR4487569..4487419PMID:21511999
three_prime_UTR4485276..4484023PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13950 Pfam IPR025308 UDP-glucose 4-epimerase C-terminal domain 287 348 2
PF01370 Pfam IPR001509 NAD-dependent epimerase/dehydratase 8 259 5
PF01263 Pfam IPR008183 Aldose 1-/Glucose-6-phosphate 1-epimerase 372 688 1
PS00545 Prosite Patterns IPR018052 Aldose 1-epimerase, conserved site 528 537 1
PTHR10366:SF39 HMMPANTHER IPR005886 UDP-glucose 4-epimerase GalE 1 360 2
PTHR10366 HMMPANTHER 1 360 7
3.90.25.10 Gene3D 276 349 3
3.90.25.10 Gene3D 188 240 3
2.70.98.10 Gene3D IPR014718 Glycoside hydrolase-type carbohydrate-binding, subgroup 371 691 1
3.40.50.720 Gene3D IPR016040 NAD(P)-binding domain 3 187 99
SSF74650 SuperFamily IPR011013 Galactose mutarotase-like domain 365 689 6
SSF51735 SuperFamily 6 346 88
TIGR01179 tigrfam IPR005886 UDP-glucose 4-epimerase GalE 8 344 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.14 Da
Charge 2.00
Isoelectric point 6.72
Molecular weight 80.67 kDa
Number of residues 713
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS706PMID:217125471663
present during mitotic M phase
present during mitotic M phaseS704PMID:21712547
S561PMID:24763107
S539PMID:24763107
S706PMID:24763107
present during mitotic M phaseS709PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased in presence of purvalanol A expression microarray evidencePMID:22840777

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
23816during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
20597during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
20561during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
21545during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
125.8during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
22364during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.14during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.14during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Genome Organisation
DescriptionQualifierReferenceCount
divergently orientated to functionally related gene10
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPBPB2B2.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPBPB2B2.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPBPB2B2.12c BioGRID Interaction Datasets
Expression Viewer SPBPB2B2.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPBPB2B2.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPBPB2B2.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPBPB2B2.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPBPB2B2.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPBPB2B2.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPBPB2B2.12c Cell Cycle Data
GEO SPBPB2B2.12c GEO profiles
PInt SPBPB2B2.12c Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz5.1.3.3Integrated relational Enzyme database
Rhea5.1.3.3Annotated reactions database
IntEnz5.1.3.2Integrated relational Enzyme database
Rhea5.1.3.2Annotated reactions database
SPD / RIKEN49/49D07Orfeome Localization Data
EntrezGene2541411UDP-glucose 4-epimerase
WikiGene2541411UDP-glucose 4-epimerase
UniProtKB/SwissProtQ9HDU3Bifunctional protein gal10
ModBaseQ9HDU3Database of comparative protein structure models
STRINGQ9HDU3Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_596857nucleotide-sugar 4,6-dehydratase (predicted)
RefSeq PeptideNP_596858UDP-glucose 4-epimerase
RefSeq mRNANM_001023880972h- nucleotide-sugar 4,6-dehydratase (predicted) (SPBPB2B2.11), mRNA
RefSeq mRNANM_001023881972h- UDP-glucose 4-epimerase (SPBPB2B2.12c), mRNA
European Nucleotide ArchiveCAC21414ENA Protein Mapping
European Nucleotide ArchiveCAC21414.1ENA Protein Mapping
UniParcUPI000012B0C8UniProt Archive

Literature for gal10

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014